서리태는 검은콩의 한 종류로 활성산소를 제거하는 항산화 효과가 있다. 주요 유효 성분은 isoflvaone이며 대표적인 isoflavone은 genistein, daidzein 등으로 12종의 isoflavone이 존재하며 화학적 구조에 따라서 배당체(glycoside)와 비배당체(aglycone)로 구분된다. 팽화는 짧은 시간에 시료를 고온고압 처리함으로써 식품의 물리화학적 성질을 변화시키는 기술로 이 연구에 사용된 pressure-drop법은 팽화기 내외부의 압력 차이를 이용해 물질 내부의 수증기압에 의한 수증기의 외부 방출로 인해 부피 팽창을 유도하는 방법이다. 본 연구는 서리태의 유효성분인 isoflavone과 항산화능이 팽화에 따라 변화되는 현상을 확인하기 위하여 다양한 압력 조건 하에서 서리태의 팽화를 진행하고 이들의 isoflavone 및 항산화능의 변화를 연구하였다. 팽화압력 조건은 490, 588, 686, 784 kPa로 진행되었으며 팽화하지 않은 sample을 control로 비교하였다. Morphology를 비교한 결과 팽화 압력이 증가할수록 껍질이 벗겨지고 표면에 균열이 생기는 현상이 심화되었다. 또한 팽화로 인해 크기가 약간 증가한 것을 확인할 수 있었지만 팽화 압력에 따른 유의차는 보이지 않았다. 팽화 후 각 sample은 70% 에탄올로 추출 후 농축을 거쳐 증류수에 녹여 사용하였다. 추출 수율은 팽화 처리에 의해 유의적인 차이를 보이지 않았다. Isoflavone 함량을 비교한 결과 팽화한 시료의 경우 control sample에 존재하던 malonyl daidzin, malonyl glycitin, malonyl genistin이 사라졌다. Acetyl daidzin, acetyl genistin, Daidzin, genistin은 팽화 압력이 증가함에 따라 증가하다가 784kPa에서 급격히 감소하였다. Glycitin, daidzein의 경우 팽화 압력에 따라 일정한 경향을 보이지 않았으며 genistein은 팽화 압력에 따라 증가하는 경향을 나타내었다. Acetyl glycitin, glycitein은 팽화와 상관없이 모두 검출되지 않았다. 팽화한 서리태의 DPPH radical, ABTS radical 소거능과 total phenolic contents는 control에 비해 증가하였고 팽화 압력에 따라 대체로 증가하는 경향을 나타낸 반면, total flavonoid contents는 일관성 있는 경향을 보이지는 않았지만 모든 팽화 조건에서 control에 비해 증가한 결과를 나타내었다. 본 연구를 통하여 팽화 처리가 서리태의 유효 성분 증가 및 변환에 긍정적인 효과를 나타내었고, 항산화능 증가를 위한 유효한 가공 처리가 될 수 있으며, isoflavone 조성을 효과적으로 변환할 수 있는 적절한 가공 방법임을 확인할 수 있었다.

목적 : 본 연구는 단축감각프로파일2(Short Sensory Profile2; SSP2)을 정상아동과 발달장애아동을 대상 으로 신뢰도와 타당도를 구하고자 하였다. 연구방법 : SSP2를 6곳에 거주하고 있는 정상아동 100명과 장애아동 100명을 대상으로 신뢰도와 타당도 를 검증하였고 신뢰도에는 내적일치도와 검사-재검사 검증을 하였으며 타당도에는 내용타당도와 판별타 당도 수렴타당도 검증을 하였다. 결과 : SSP2의 감각처리영역의 내적일치도는 .89로 확인되었으며 감각처리와 관련된 행동반응영역은 .92 를 확인되었다. 검사-재검사 신뢰도의 감각처리영역은 .89에서 .90이었으며 감각처리와 관련된 행동반 응영역은 .80~.97로 유의한 상관관계를 나타내었다. 정상아동과 장애아동을 비교한 판별타당도에서도 통계적으로 유의미한 차이를 보였다. 마지막으로 SSP2와 SSP를 비교한 수렴타당도의 감각처리영역의 총 점수간의 계수는 .85이고 감각처리와 관련된 행동반응영역의 총 점수간의 상관계수는 .85로 높은 상 관관계를 보였다. 결론 : 본 연구는 SSP2의 신뢰도와 타당도를 확인하였으며 연구 결과 높은 수준을 보였으므로 SSP와 같 이 임상에서의 사용이 용이할 것으로 판단된다.

One of the challenges facing precision manufacturers is the increasing feature complexity of tight tolerance parts. All engineering drawings must account for the size, form, orientation, and location of all features to ensure manufacturability, measurability, and design intent. Geometric controls per ASME Y14.5 are typically applied to specify dimensional tolerances on engineering drawings and define size, form, orientation, and location of features. Many engineering drawings lack the necessary geometric dimensioning and tolerancing to allow for timely and accurate inspection and verification. Plus-minus tolerancing is typically ambiguous and requires extra time by engineering, programming, machining, and inspection functions to debate and agree on a single conclusion. Complex geometry can result in long inspection and verification times and put even the most sophisticated measurement equipment and processes to the test. In addition, design, manufacturing and quality engineers are often frustrated by communication errors over these features. However, an approach called profile tolerancing offers optimal definition of design intent by explicitly defining uniform boundaries around the physical geometry. It is an efficient and effective method for measurement and quality control. There are several advantages for product designers who use position and profile tolerancing instead of linear dimensioning. When design intent is conveyed unambiguously, manufacturers don’t have to field multiple question from suppliers as they design and build a process for manufacturing and inspection. Profile tolerancing, when it is applied correctly, provides manufacturing and inspection functions with unambiguously defined tolerancing. Those data are manufacturable and measurable. Customers can see cost and lead time reductions with parts that consistently meet the design intent. Components can function properly-eliminating costly rework, redesign, and missed market opportunities. However a supplier that is poised to embrace profile tolerancing will no doubt run into resistance from those who would prefer the way things have always been done. It is not just internal naysayers, but also suppliers that might fight the change. In addition, the investment for suppliers can be steep in terms of training, equipment, and software.

In this work, the natural convection in an annulus between two concentric cylinders is studied numerically. The fluid flow between the cylinders is solved by the lattice Boltzmann method (LBM) while a separate finite difference method (FDM) is used to solve the heat transfer. No-slip and constant boundary conditions at curved boundaries of the cylinders are treated with a smoothed profile method (SPM). At first, the velocity and temperature profiles obtained from the present LBM-SPM and FDM-SPM are validated with the corresponding theoretical results. Later, natural convection simulations inside the annulus are performed using coupled scheme of LBM-FDM-SPM by varying Ra in the range Ra=1000, Ra=10000, Ra=50000, and Ra=100000. From the temperature and fluid flow patterns obtained at different Ra, it is found that the heat transfer is mainly dominated by conduction process when Ra is low and by convection process when Ra is high.

본 연구는 다양한 농업 환경에서 채집된 파리의 분비물에서 E. coli을 분리하고 분리된 E. coli의 병원성유전자 및 항생제내성을 조사하기 위하여 수행되였다. 파리는 과일농장(n = 19), 장류생산농장(n = 9), 생활쓰레기 야적장(n = 46), 축사(n = 66), 도축장(n = 38), 퇴비장(n = 10)에서 총 188 마리를 채집하여 토사물과 배설물로부터 E. coli을 분리 및 동정하였다. 그 결과, 채집된 파리의 63%(119/188)에서 E. coli이 검출되었으며 특히 도축장에서 채집된 파리에서 E. coli의 검출률이 89%(34/38)로 가장 높았다. 또한 분리된 E. coli을 대상으로 병원성 유전자 8종(ST, LT, VT1, VT2, aggR, bfpA, eaeA, ipaH)을 조사한 결과, 도축장에서 채집 된 파리에서 분리된 E. coli 중 91%(31/34)가 장독소를 생산할 수 있는 ST유전자를 보유하고 있었다. 분리된 E. coli 의 16%(31/188)가 1종 이상의 항생제에 내성을 보였다. 특 히, 항생제사용빈도가 높은 축사에서 채집된 파리의 E. coli 경우에는 59%(23/39)가 항생제 내성을 나타내었다. 분 리된 항생제 내성 E. coli 균주 중 10%(12/119)는 2종 이 상의 항생제에 내성을 보였고, 모두 축사 채집 파리에서 분리된 균주였으며, 이 중 2개 균주는 다재내성의 지표인 ESBL (Extended-spectrum beta-lactamase)에 양성을 나타내었다. ESBL 양성균주 중 1 균주는 7종의 항생제에 내성 을 보였이는 것으로 조사되었다. 본 연구의 결과, 축산환 경 서식 파리에서 분리된 E. coli은 병원성 유전자를 보유 하고 있을 가능성이 높을 뿐만 아니라 항생제에 내성을 나타낼 가능성도 높기 때문에 농식품을 생산하는 농장이나 식품공장은 가급적 축산환경으로부터 일정한 거리를 두거나 방충망 등의 차단조치가 필요할 것으로 판단된다.

The release profiles of nisin from nisin-incorporating gel foods and the antimicrobial activities of the gels on the growth of Brochothrix thermosphacta in an aqueous system containing the gels have been investigated. A linear regression model was applied to determine the diffusion coefficient (D) for the diffusion of nisin in the gel. The antimicrobial activities of nisin released from 1, 2 and 3% (w/v) agar gels on the growth of B. thermosphacta in a broth medium with and without nisin were investigated. The D decreased from 1.2×10 -2 to 8.2×10 -3 and 6.4×10 -3 cm 2 /s as the agar concentration in the gel increased from 1 to 2 and 3% (w/v), demonstrating the diffusion rate in the gels can be controlled by the agar concentration in gel. The agar gel incorporating nisin inhibited the B. thermosphacta growth in the broth medium by prolonging the lag phase. The growth inhibition was enhanced by the addition of nisin in the medium. The results of this study exhibited that the gel food is a feasible nisin delivery system with a controlled release achieved by the adjustment of agar concentration in the system, demonstrating the potential of nisin-incorporating gel for preserving particulate-containing drinks.

As manufacturing industries become globalized, product design affects every area of organization. The design sets the goals for a number of different departments, so if it fails to effectively communicate these goals, the entire organization is less efficient. In addition, To communicate clearly, the design must represent a product that meets its technical specification.GD&T (Geometric Dimensioning and Tolerancing) is one of the most important factors, which has an effect on efficiency of manufacture system, in designing products. However, most of designers in different industries are prone to ignore the importance of GD&T.To analyse the importance of GD&T compliance with international standards for design drawing, a comparison analysis of the difference between two methods, composite profile control and multiple single segment profile control, is performed on three different cases and suggests how it used to be more suitable.Composite profile tolerance is specified by a dual feature control frame that has one profile symbol specified with two lines of tolerance information. Whereas a multiple single segment profile control is when two or more single segment profile callouts are used to define the location and/or orientation and/or size and/or form of a part feature. In this study, the following results will be provided : a clear definition and an obvious difference of the tolerance zone, datums and datums sequence and minimization of tolerances.On this study, composite profile tolerance and multiple single segment profile tolerance were discussed. Next steps of research will consist on reaching more accurate results for profile control. Further research will be focused on dealing with the remaining 14 symbols of GD&T.

This research was conducted to evaluate quality changes in traditional Doenjang and manufactured Doenjang during a storage period of 8 weeks. Low-salt Doenjang and commercial Doenjang were purchased from different manufacturers and proximate analysis as well as changes in isoflavone, polyphenol, flavonoid contents of the samples were investigated using a mass spectrophotometer. The salinity of traditional Doenjang, low salt Doenjang, and commercial Doenjang were 13.2±1.15, 7.17±2.74, 10.67±0.35%, respectively and the salt concentrations of the soybean pastes did not change during storage. After 8 weeks at 35℃, chromatic values of all the paste samples decreased somewhat, with traditional Doenjang exhibiting fewer changes as compared to manufactured Doenjang. Amino acid nitrogen, acidity, microbial population all tended to increase with time, although some samples showed fluctuations during the test period. Moreover, the total isoflavone contents of traditional Doenjang increased with storage time while that of manufactured Doenjang tended to decrease. The isoflavone aglycone was shown to be the highest in traditional Doenjang, while isoflavone glycoside was abundant in manufactured Doenjang. Total flavonoid contents showed similar trends regardless of samples; initial contents of total flavonoid was 0.6 mg/g which increased to more than twice to 1.4 mg/g at the end of storage period. Composition profile of Doenjang extracts was analyzed using UPLC-Q-ToF

덕트에 설치되는 오리피스는 압력과 유량 제어용으로 많이 활용되고 있다. 선박의 거주 구역에 통풍 덕트는 환기와 배기를 위해 설치되며 및 선박의 설계 및 건조 후 검사는 공기 유속과 유량 그리고 소음 기준에 대해 수행된다. 이 연구에서는 선박의 선실 별 유량분배에 중요한 요소인 T-분지관을 대상 으로 오리피스를 적용한 경우 난류운동에너지 분포를 고찰하였다.

During tick infestation, the tick secretes bioactive substances that modify the host’s physiological and immunological reactions. The study of tick saliva is important to understand tick biology as tick saliva plays a special physiological role in pathogen transmission. The average salivary protein concentration was found to be 0.169 μg/μl/tick and saliva secretion decreased with increased time of tick detachment from the host. Saliva secretion volume increased to 3.56 μl in the group of ticks with a body weight between 301–350 mg as compared to higher and lower body weight groups. On-chip-electrophoresis results show 13 distinct bands ranging from 9.9 to 294 kDa. For salivary protein LC-MS/MS was performed. A total of 135 tick salivary proteins were identified of which 30 proteins were found exclusively in fully engorged nymph saliva, 74 in fully engorged adult females, and 31 were detected in both stages. Results of this may help researchers to identify tick proteins as potential candidates for further studies aimed to develop novel tick control strategies to affect both the ticks and the pathogens transmitted by them.

본 연구는 치유의 숲에서 다른 유형의 소리(무음, 자연, 음악, 아이들 노는 소리)가 방문객의 기분상태(Profile of Mood States: POMS)에 미치는 영향을 비교하였다. 자료는 2012년 10월 중 장성 ‘축령산편백치유의 숲’ 내에 위치한 ‘치유필드’를 이용하는 231명의 방문객으로부터 수집되었다. 각 피실험자는 각기 다른 유형의 소리에 노출되었으며 실험용 소리가 장착된 헤드셋을 착용한 상태에서 설문에 답변하였다. 실험결과, 기대했던 대로 ‘아이들 노는 소리’ 노출 그룹이 타 그룹에 비해 높은 부정적 기분상태를 나타냈다. 그러나 예측과 달리 자연소리와 음악소리는 피실험자의 POMS에 영향을 미치지 않았다. 이러한 경향은 총 기분장애 점수(Total Mood Disturbance Score: TMDS)뿐만 아니라 요인별(불안, 분노, 활력, 피로, 혼란, 우울) POMS에서도 동일하게 나타났다. 또한 혼잡지각(Perceived Crowding)수준 과 TMDS(r=0.568) 및 하위 요인별 기분상태(r=0.331-0.571)간에 정적 상관관계가 있었다. 연구의 결과에 대한 토의와 더불어 관리적 차원에서 연구결과에 대한 시사점을 제시하였다.

In this study, we determined the effect of the mulberry leaf powder on blood lipid metabolism and liver function improvement of mice fed a high fat diet. The mulberry leaf showed a moisture content of 10.74±0.56%, ash 9.67±0.56%, crude protein 25.24±1.02%, and crude fat 2.66±0.08%. In the group with high fat diet supplemented with mulberry leaf powder, serum low-density lipoprotein (LDL-cholesterol) and total cholesterol levels were significantly lower (p<0.05); also, serum phospholipid and aspartate amino transferase (AST) levels were significantly lower (p<0.05). Serum insulin and leptin levels were high in mice fed a high fat diet; however, addition of mulberry leaf powder 10% in the diet had no significant effect on blood insulin and leptin control. The results of the study suggested that various plant sterols in mulberry leaf improve lipid metabolism and liver function in ICR-mice.

본 논문은 히스토그램과 그레이레벨 프로파일의 VS(valley size)를 이용한 문턱치 기반의 대두 선별알고리즘 을 제안한다. 대두의 변형은 크게 표면 변색 및 외형 변형으로 나눌 수 있다. 본 연구에서는 대두 개체를 건전립, 부 분변색립, 전면변색립, 표면변형립으로 구분하고 히스토그램 기반 문턱치와 VS기반 선별알고리즘을 이용하여 표면의 변색 및 변형에 따른 불량 대두와 건전립을 선별하였다. 제안한 방법은 대두와 같이 개체수가 많은 농작물의 실시간 선별을 목적으로 하여, 정수덧셈 연산기반의 선별 알고리즘으로 구현 되었다. 제안한 방법을 실제 선별에 적용한 결 과 선별의 정확도는 94.5%이며 기존의 문턱치 기반 대두 선별 알고리즘에 비교할 때 상대적으로 적은 연산량에서 동 등한 수준의 정확도를 나타내는 것을 확인하였다.

The present study investigated the effects of fermented potato protein (FPP, Lianol® ferity) during gestation and lactation on productivity of sows. A total of 50 crossbred sows (Landrace×Yorkshire) in their 3 to 5 parities were allotted to one of two treatments (n=25) including control and FPP groups. FPP tablets applied in sows in two stages. Stage one involved applying FPP daily from 3 days before farrowing to 2 days after for 5 consecutive days. The second stage also involved a 5 day period around weaning time from 3 days before to 2 days after. After farrowing, the amount of feed offered 3 times per day gradually increased from about 3.6 kg at farrowing to 8.4 kg at late lactation. During the first lactation FPP tended to increase backfat thickness (BFT) at weaning at the first (p=0.069) whereas FPP increased BFT (p<0.05) at weaning in the second lactation. There was no significant effect of FPP on body weight changes and daily feed intake of sows. Decreased weaning to estrus interval was associated with applying lianol tablet at the second lactation (p<0.05). Weight of born alive piglets, weaned piglets and total weight gain were greater in FPP group at the second lactation (p<0.05). Applying FPP tended (p=0.062) to increase insulin like growth factor-Ⅰ(IGF-I) at the weaning time in the first lactation. The effect of FPP on IGF-I was significant at the second lactation, revealing a higher concentration in blood at post farrowing and weaning time (p<0.05). This study shows the benefit of using FPP tablets in sows to increase blood IGF-I and both initial and final litter size to improve piglet weaning weights.

This study was conducted to determine the association between dietary calcium intake and biomarkers related to lipid and glucose metabolism and inflammation in Korean patients with type 2 diabetes. Seventy-five subjects (41 males, 34 females) were recruited from a group of patients who had visited the department of endocrine medicine. Data on anthropometric characteristics, clinical indices such as hemoglobin A1c and C-reactive protein (CRP), and dietary nutrient intakes were collected. Subjects were divided into three groups on the basis of their calcium intake [<EAR (below estimated average requirement), EAR-RNI (between EAR and recommended nutrient intake), >RNI (above RNI)]. Average calcium intake of <EAR, EAR-RNI, >RNI groups were 462.7±18.7, 649.7±12.8, and 895.7±21.7 mg, respectively. Energy intake was not different among groups but intakes of protein, total and saturated fatty acids were significantly higher in >RNI group than <EAR group. Analysis of covariance revealed that HDL cholesterol level was significantly higher in EAR-RNI group, as compared to <EAR group after adjustment with confounders such as age, sex, BMI and energy intake (p<0.05). Levels of CRP and homeostasis model assessment 2-insulin resistance (HOMA2-IR) were significantly lower in EAR-RNI group. Total cholesterol level was higher in EAR-RNI and >RNI groups, although within the normal range. Our results suggest that dietary calcium intake may influence the levels of HDL-cholesterol, CRP and HOMA2-IR and subsequently, help management/treatment of type 2 diabetes patients.