The purpose of this study was to investigate the effects of extract mixture of C. wilfordii and P. umbrosa (IPLUS-CWPU) on bone growth in 4-week old young male SD rats. To confirm the effect of IPLUS-CWPU, we measured the length of bone growth plate, the ratio of proliferative zone to the length of growth plate and the expression level of insulin-like growth factor, IGF-1. The IPLUS-CWPU treatment shows a significant increase of tibial and femoral growth plate and the ratio of proliferative zone in growth plate. Especially, the length increased by 13.9% and 25.3% in the tibia and femur, respectively, in the high-dose group compared to the normal group. Moreover, the expression of IGF-1 gene in liver was upregulated in IPLUS-CWPU treated groups. These results indicated that IPLUS-CWPU administration could increase the proliferative zone of bone growth plate in early developmental stage by upregulation of IGF-1 gene.

본 연구에서는 건강기능성 식품원료로서 이용 빈도가 증가하고 있는 하수오, 백수오 및 이엽우피소에 대한 종 특이 프라이머를 개발하였다. 개발된 종 특이 프라이머는 하수오, 백수오 및 이엽우피소에 대해 원물뿐만 아니라 육안 확인이 어려운 가공식품 등을 대상으로 사용원료의 진위여부를 빠르고 정확하게 확인할 수 있었다. 따라서 본연구에서 개발한 종 특이 PCR방법은 기존의 일반 프라이머를 사용하는 방법이 가지고 있는 식품 적용 한계를 극복할 수 있을 것이라고 판단하였다.

This studies were conducted to develop cultivation methods on three Asclepiadaceae fami-lies(Cynanchum wilfordii Hemsley, C. auriculatum Royle ex Wight and Metaplexis japonica Makino) ofwhich products are conventionally used as medicinal materials by analyzing plant growth. The variationof leaf weight of Asclepiadaceae families showed culmination point at August 15, and the stem showedhighest growth on August 30. The root weight increased linearly during the growth. The days for sprout-ing was most shortened in the Cynanchum wilfordii as 18 days, and C. auriculatum, Metaplexis japonicafollowed after. The seed harvesting dates was Oct. 8 in the Cynanchum wilfordii, Oct. 23 in the Metaplexisjaponica, and Nov. 1 in the C. auriculatum, respectively.

야외 포장에서 십자무늬긴노린재의 연중 발생소장 및 월동, 식이 선호성 등을 조사하여 얻은 결과는 다음과 같다. 시기별 발생소장은 5월 상, 중순에 월동성충이 출현하여 6월 하순부터 8월 중순까지 1세대성충이 출현하고 9월 중순에서 10월 상순에 2세대성충이 출현하였다. 이 2세대성충이 토양 속이나 낙엽밑에서 월동하였다. 십자무늬긴노린재는 백열등(100W), 청광등(BL:20W), 흑광등(BLB:20W)의 유아등에는 모두 유인되지 않았다. 식이 선호성은 박주가리과의 박주가리와 백하수오(큰조롱)에 기주특이성을 보였다.

Background : This study provides Profitability Analysis information on Baeksoooh (Cynanchum wilfordii), One of the Crops excluded from the production cost survey. and it will contribute to increase the income of farmers. The purpose of this study was to survey and analyse the farm business performance of new economic crops cultivated in small area. Method and Results : In this study, we selected Baeksoooh, which is also called Baekhasuo, as a subject of investigation, based on the roots of C. wilfordii Hemsley. We visited 20 farmers who cultivated Baeksoooh in Jecheon, we surveyed the quantity, price of farmhouse receipts, cost of each branch, the status of agricultural machinery and facilities, and We analyzed income, operating expenses, and income per unit area. and also We set up a proper management model. It can be cultivated in all regions of Korea, but it is the main production area of Jecheon in Chungcheongbuk-do under the cool climate conditions. Most are being traded for medicinal uses. Looking at the profitability per 10a by Baeksooh's business performance, The result of this study are follows as. In the case of the top 20% farms, the gross quantity of the C. wilfordii is 332 ㎏ per 10a, the gross income was 11,418, managing cost was 2,259, income was 9,158 thousand won per 10a. In the average farms surveyed, the gross quantity of the C. wilfordii is 205 ㎏ per 10a, the gross income was 4,611, managing cost was 2,418, income was 2,193, production cost was 3,169 and net income was 1,445 thousand won per 10a. In the lower 20% farms cultivation, the gross quantity of the C. wilfordii is 46 ㎏ per 10a, the gross income was 89, managing cost was 1,848, income was – 1.759 thousand won per 10a. The professional farm management scale based on Baeksoooh's income of 20 million won is 43.4 a, it can produce 890 ㎏ with 1,176 hours per years, and the income is 9,522 thousand won Conclusion : Because the Baeksoooh being processed and circulated as health functional food have raised questions about their feasibility, Production and consumption declined sharply. but Consumption is expected to increase gradually and it is likely to grow, but prices are expected to fluctuate. Therefore, The bottom 20% farmers should benchmark the top 20% farms and cultivate the appropriate scale. and It is necessary to cultivate in consideration of supply and demand of manpower as well as proper size and cultivation.

Background : To develop a new cultivation technique which can save the labor and harvest cost in Cynanchum wilfordii. Stable production is possible for soil and container cultivation. Therefore, this test was conducted to evaluate the reuse effect that can reduce the cost of culture soil. Methods and Results : Container standards were used plume tube of diameter 30 ㎝ × height 50 ㎝. The blending ratios of reuse cultivation soil were 60% cocopeat, 10% peatmoss, 6% pearlite, 6% vermiculite, 5% zeolite, and 5% char. To this, 0, 10, 20, and 30% of composts containing 45% of fowl droppings and 5% of cattle dung were added to the culture soil and mixed. In each treatment C. wilfordii seedling were planted, and the overground growing and yield ability were examined. After harvesting, the functional components contents (cynanoneside B, wilfoside C1N, and p-hydroxyacetophenone) of C. wilfordii conventional cultivation and container cultivation (culture soil) were analyzed. As a result, the growth of overgrown crops showed better tendency as the compost content increased. However, the growth and yield of undergrowth increased by 182% (164.7 ㎏/10 a → 282 ㎏/10 a) compared to no addition at 20% mixing ratio. The content of functional ingredients according to cultivation method and skin was not different all in cynanoneside B, wilfoside C1N, and p-hydroxyacetophenone. Therefore, it was a larger tendency that the classification according to whether it was skined (existence and nonexistence) than the cultivation method. In the economic analysis, it was found that when the cultivation soil was reused by adding 20% compost, the profit of 680,000 won/10 a was increased. Conclusion : Soil and container cultivation was effective saving method for harvesting ease of C. wilfordi, harvest yield was increased by 66%. Also, container cultivation was effective the cost cutting of installation dismantlement and redres since no need to move the cultivation area. As the cultivation soil can be reused, it can be expected to maximize the utilization in container cultivation of deep rooting medicinal crops as well as Cynanchum wilfordii.

Background : Cynanchum wilfordii is a perennial species of climbing vine belonging to the family Asclepiadaceae. Cynanchum wilfordii native to Korea has been cultivated and used for medicine with tuber root. Prevalent furrow size is 20 ㎝ in height for Cynanchum wilfordii cultivation in Korea. This experiment was carried out to find out yield and commercial characteristics according to furrow height in cultivation of Cynanchum wilfordii. Methods and Results : There were 4 furrow height of 10 ㎝, 20 ㎝, 30 ㎝, and 40 ㎝. Seedling transplanting was performed in early May with 40-day seedlings grown in pot from late March. Seedling establishment rates were similar as 96.7% - 99.7% in all treatments. Vine length, branch number, node number, stem diameter, leaf length, and leaf width were higher in furrow of 40 ㎝ and chlorophyll content was not significantly different according to furrow height. Main root number was more as 5.2 ea/plant in 20 ㎝ furrow and first lateral root number was more as 4.5 ea/plant but not significantly different in 20 ㎝ and 30 ㎝ furrow. Main root length was longer as 39.2 ㎝ and 37.0 ㎝ in 40 ㎝ and 30 ㎝ furrow height respectively. First lateral root length was longest as 25.0 ㎝ in 30 ㎝ furrow. Main root diameter was thicker as 24.9 ㎜ in 40cm furrow and thinner as 18.7 ㎜ in 10 ㎝ furrow. First lateral root diameter was thicker as 14.4 ㎜ in 20 ㎝ furrow and thinner as 11.2 ㎜ in 10 ㎝ furrow. Total yield and commercial yield in 40 ㎝ furrow were highest as 1,173 ㎏/10 a and 919 ㎏/10 a, 78.6% of total yield. Conclusion : It was considered that higher total yield and commercial yield in 40 ㎝ furrow was resulted from better aerial part growth including vine length, branch number, node number, stem diameter, leaf length, and leaf width and bigger main tuber root size.

Background : Cynanchum wilfordii is a perennial species of climbing vine belonging to the family Asclepiadaceae. Cynanchum wilfordii native to Korea has been cultivated and used for medicine with tuber root. Direct seeding and seedling transplanting and tuber root seed (below ∅10 ㎜) planting have been performed for cultivation. This experiment was carried out to investigate growth and yield characteristics of Cynanchum wilfordii according to planting method. Methods and Results : Direct-seeding and tuber root seed (TRS) planting were performed in early April and seedling transplanting with seedling grown for 40 days was performed in early may. TRS size was 2 – 3 ㎝ in length and 10 ㎜ and 20 ㎜ in diameter respectively. Seedling establishment rate was similar as 97.3% - 99.0% in all treatments. Vine length, branch number, node number and chlorophyll content were not significantly different in all treatments. Stem diameter, leaf length, and leaf width were higher in TRS planting. Main root number was more as 4.5 - 4.3 in seedling planting and TRS planting (∅20 ㎜) respectively but first lateral root number was not significantly different in all treatments. Root diameter was thicker as 23.3 ㎜ in TRS planting (∅20 ㎜). Total yield in TRS planting (∅20 ㎜) was higher as 1,041 ㎏/10 a, 25% increased compared to 830 ㎏/10 a of direct seeding and with higher commercial yield rate of 75.7%. Conclusion : It was considered that higher total yield and commercial yield in TRS planting (∅20 ㎜) was resulted from bigger size of stem diameter, leaf length, and leaf width and bigger main root size related to root yield and commercial value.

Background : This experiment was carried out to investigate the occurrence of disease and pest and the yield depending on cultivation methods in Cynanchum wilfordii. Methods and Results : There were damages by anthracnose, aphid, mite, thrips, Gastrophysa atrocyanea, Tropidothorax cruciger and Metcalfa pruinosa. In the case of anthracnose, the number of damaged leaf in the untreated control plot per counting area (67.5 ㎡) was 8.6 times higher than that of chemical treated plot. As a result of investigation of shoot damage and leaf juice damage by Tropidothorax cruciger, which made the most obvious damage symptom, showed the significantly lower degree of damage in the treatments of insecticides (abamectin, suloxac flour liquid wettable powder). In addition to the pest damage, the yield of roots was investigated. As a result, it was found that the yields were 3.8 ㎏, 2.6 ㎏, 1.7 ㎏ and 1.4 ㎏ per 8.5 ㎡ respectively in vine scheme cultivation with chemical control, vine scheme cultivation with non-chemical control, non-vine scheme cultivation with chemical control and non-vine scheme cultivation with non-chemical control. Conclusion : The yield per 1,000 ㎡ was 449 ㎏, which was 1.9 times higher than the national average of three years in vine scheme cultivation with chemical control.

Background:The study aimed to obtain data on the effects of cultivation and soil reduction of green manure crop on the quantity and quality of organically cultivated Cynanchum wilfordii Hemsley.Methods and Results:The experiment comprised four treatments: control, hairy vetch, barley, and hairy vetch + barley (3 : 2). The plant height in the hairy vetch treatment (86.3㎝) was significantly different from that in the other treatments, whereas the stem diameter leaf area, and special product analysis division (SPAD) value did not differ across the treatments. The largest soil reduction of green manure crop was recorded in the barley treatment (440 ㎏/10 a), whereas the smallest was recorded in the single treatment with hairy vetch (80 ㎏/10 a). The hairy vetch + barley (60 : 40) treatment showed 63% more soil microorganisms than control. Radical scavenging activity estimation revealed that the total polyphenol content was highest (1,740 ㎎/㎏), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 92.6% in the barley treatment. The 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activation was highest in the control (51.1%), and the root yield was the highest in the barley treatment (310 ㎏/10 a).Conclusions:The root yield, total polyphenol content, and antioxidant activity of Cynanchum wilfordii (Maxim.) Hemsley increased in presence of the green manure crop barley.

Cynanchum wilfordii Radix (CWR), Arctium lappa L (ALL), and Dioscorea opposite (DO) have been known to improve blood lipid profile, blood pressure, and inflammation. To find the optimal combination ratio of CWR, ALL, and DO in terms of vascular health improvement, we compared the effects of various combinations on gene expression of Vascular cell adhesion protein 1 (VCAM-1) in human aortic smooth muscle cells (HASMC). VCAM-1 mediates endothelial leukocyte adhesion and is upregulated in atherosclerosis. Cells was stimulated by TNF-α (10 ng/㎖, 2h) and treated with various combinations for 24 h. A combination (CADM5, CWR:ALL:DO = 2:1:1) showed the strongest suppression of VCAM-1 so that CADM5 was chosen for further experiments. We performed cell viability test with CADM5 (0, 3.125, 12.5, 25, 50, and 100 ㎍/㎖) and no cytotoxicity was found. We also investigated the effect of CADM5 on protein expression of VCAM-1, ICAM-1, Nrf-2, and HO-1 using western blotting. We found that CADM5 diminished the expression of VCAM-1 and increased the expression of Nrf-2 and HO-1. Therefore, we concluded that CADM5 (CWR:ALL:DO = 2:1:1) effectively improves vascular health by regulating the expression of VCAM-1.

Background : Cynanchum wilfordii and Cynanchum auriculatum belong to the Asclepiadaceae family and appear morphologically similar. In order to discriminate them, it is needed to find the presence of sap and the leaf shapes: C. auriculatum has a blade ovate leaf comparing to C. wilfordii. However, in the herbal medicine market, they have been handled as cut and dried roots. Due to their similar morphology, it is limited to distinguish the roots of C. wilfordii and C. auriculatum. Recently in Korea, it has been a critical issue to misuse these two roots in the herbal market and food industry. Thus, it is required to establish a robust tool for the discrimination and quality control of them. Methods and Results : To separation and characterization of flavor compounds, C. wilfordii and C. auriculatum samples were analyzed by head space solid phase micro extraction (HSS) fiber coupled with gas chromatography-mass spectrometry using Rtx-5MS (30 m x 0.25 mm x 0.25 μm) column. As a result, We have identified compounds of a few hundred in aliphatic aldehydes and aliphatic alcohols, alkenes and acids, aromatic compounds, aromatic compounds containing nitrogen & sulfur, etc,. In particular, The aliphatic and aromatic compounds had been clearly separated on the second dimensional direction by using two-dimensional GC. Conclusion : The volatile flavor compounds of C. wilfordii and C. auriculatum could easily analyzed without pre-treatment with improved resolution and sensitivity using HSS-GCxGC-TOFMS. We have identified compounds of a few hundred in C. wilfordii and C. auriculatum sample. And It was more accurately qualitative confirmed with separation of GCxGC and TSD. We have confirmed the PCA and PLS-DA Plot that was classified depending on C. wilfordii and C. auriculatum through multivariate statistical analysis of the identified flavor compounds.