collected from approximately 1100 purebred Duroc pigs between 2011 and 2017. The meat quality traits evaluated were: moisture content (MC), fat content (FC), water holding capacity (WHC), cooking loss (CL), and shear force (SF). Meat samples were evaluated for the following fatty acids: palmitic, stearic, oleic, linoleic, and linolenic acids. We evaluated the meat for the following sensory traits: color, flavor, tenderness, juiciness, and acceptability of the meat. Variance and covariance components were estimated using restricted maximum likelihood procedures on different animal models. The results showed that the estimates of heritability for MC, FC, WHC, CL, and SF were 0.17, 0.58, 0.34, 0.31, and 0.42, respectively. The heritability estimates for fatty acid composition were moderate to high (0.34 to 0.63). The estimates of genetic correlation were -0.60 and 0.46 between MC and FC, and MC and SF, respectively. Generally, phenotypic correlation between meat quality traits was low. Linoleic acid had moderate to high negative phenotypic and genotypic correlation with both palmitic and oleic acid. Comparison of meat quality traits and sensory traits revealed a positive correlation (0.208) between acceptability and WHC, while the correlation between acceptability and SF was negative (-0.207). The estimated genetic parameters among meat quality traits, sensory traits, and fatty acid composition in this study are expected to be used to improve pork to suit consumer preferences.

본 연구는 두록종 돼지의 복당 생시체중 형질(복당 생시체중 평균, 복당 생시체중 표준편차)과 산자수 형질(총 산자수, 생존 산자수, 사산두수)의 유전모수를 추정하고 이들 형질간의 상관관계와 개량방안을 알아보기 위해 실시하였다. 분석에는 국립축산과학원에서 2006~2016년 사이에 분만한 두록 모돈 1,081복의 자료를 이용하였다. 분석형질은 복당 생시체중 평균(ABW), 복당 생시체중 표준편차(BWSD), 총 산자수 (NB), 생존 산자수(NBA) 및 사산두수(NBD)이다. 유전모수 추정은 다형질 개체모형을 이용하였다. 각 형질에 대한 유전력은 복당 생시체중 평균(ABW), 복당 생시체중 표준편차(BWSD), 총 산자수(NB), 생존 산자수(NBA) 및 사산두수(NBD)에 대해 각각 0.32, 0.13, 0.11, 0.10, 및 0.06으로 추정되었다. 복당 생시체중 평균(ABW)과 복당 생시체중 표준편차(BWSD)는 0.75의 높은 정의 유전상관을 보인 반면 생존 산자수(NBA)와 복당 생시체중 평균(ABW), 복당 생시체중 표준편차(BWSD)간의 유전상관은 각각 –0.26, -0.40로 생존 산자수(NBA)가 증가함에 따라 복당 생시체중 평균과 복내 개체간 체중의 표준편차는 감소하는 것으로 나타났다. 생존 산자수(NBA)와 사산두수(NBD)간의 유전상관은 0.24로 생존 산자수(NBA)가 증가하면 사산두수(NBD)도 증가하는 유전적 관계를 나타내었다. 한편 복당 생시체중 평균(ABW)과 복당 생시체중 표준편차(BWSD), 사산두수(NBD), 총 산자수(NB)간의 유전상관은 각각 0.75, 0.81과 0.08로 추정되어 복당 생시체중 평균(ABW)이 증가하면 총 산자수(NB)의 큰 변화 없이 복당 생시체중 표준편차(BWSD), 사산두수(NBD)가 증가하는 것으로 나타났다. 본 결과로 미루어 볼 때 유전력이 높은 복당 생시체중 평균(ABW)은 직접선발을 통한 빠른 개량이 가능한 반면 복당 생시체중 표준편차(BWSD), 총 산자수(NB), 생존 산자수(NBA) 및 사산두수(NBD) 형질은 직접선발을 통한 개량 효과가 낮을 것으로 판단된다. 특히 자돈 생시체중의 균일도를 개량하고자 한다면 복당 생시체중 표준 편차(BWSD)에 대한 직접선발 방법과 더불어 복당 생시체중 평균(ABW)을 이용해 간접선발하는 방법 또한 이용 가능할 것으로 사료된다.

ZAR1은 척추동물의 초기 배아 발달에 영향을 미치는 유전자로 알려져 있다. 본 연구는 두록 수퇘지 112두에서 ZAR1 유전자의 SNP을 발굴하고 ZAR1 유전자의 인트론 Single nucleotide polymorphism(SNP)과 정액의 운동학적 특성들과의 연관성을 규명하였다. 돼지의 정액 운동학적 특성을 분석하기 위해서 운동성(motility, MOT), 직선운동 속도(straight-line velocity, VSL), 곡선운동 속도(curvilinear velocity, VCL), 평균운동 속도(average path velocity, VAP), 직진성(linearity, LIN), 선형성(straightness, STR), 측두 이동거리(amplitude of lateral head displacement, ALH) 및 머리 진동수(beat cross frequency, BCF)를 측정 하였다. SNP을 탐색하기 위해서 돼지의 혈액에서 genomic DNA를 추출하여 PCR(polymerase chain reaction)을 통해 시퀀싱 분석을 하여 유전자형을 분석하였다. 그 결과 ZAR1의 non-coding 영역인 인트론에서 SNP 3개를 확인 했으며 각 각 인트론 2 영역에서 g.2435T>C, 인트론3 영역에서 g.2605G>A, g.4633A>C 변이를 보였다. g.2435T>C, g.2605G>A는 각 각 MOT(p<0.01)와 VSL(p< 0.05)에서 높은 연관성을 나타냈고 g.4633A>C는 MOT, VCL, VSL, VAP, ALH에서 높은 연관성을 나타냈다(p<0.01). 본 연구 에서는 ZAR1 유전자의 SNP이 돼지 정액의 운동학적 특성들에 영향을 미치는 것으로 나타났다. 이러한 결과는 ZAR1이 우수한 수퇘지들에서 번식능력이 높은 개체를 선발할 수 있는 후보유전자로 제시하며 다양한 돼지의 품종과 더 많은 두수를 확보하여 검증연구를 통해 우수한 능력의 수퇘지에서 활력이 좋은 개체를 선발하는 분자마커 연구의 기초자료로 사용이 가능하다.

Precise determination of insemination time is an important factor. However, the detection is intensive labor observing behaviors of sows. Previous researchers have tried to find indicators for easy detection of optimum time of insemination. The one of the indicators is change of temperature for estrous cycle. Therefore, this study was conducted to determined alteration of vulvar and rectal temperature in Duroc sows during estrus period for detection of optimum time of insemination. Experimental animals were twelve Duroc sows 2 parity for winter season. The sows were feeding 5 ml regumate (estrus synchronization agent) during 15 days, and then measured temperature of vulva and rectum when half day by thermal camera and thermometer. Also, conventional method was utilized as control. By the conventional method, estrus was identified at four days after ending supply of regumate. At the same time, temperature of the vulva and rectum was higher than other days. Average temperature in valva and rectum was 38.8 ℃ in estrous sows, wherease non-estrous sows were 38.7℃. This difference of temperature suggested feasibility as indicator inseminating optimum time. Henceforward, ummanned estrous detection system can be useful by economic improvement via reduction of labor in swine farm.

For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Estrogen receptors 2(ESR2) is involved in estrogen related apoptosis in cell cycle spermatogenesis, but their functions have not been confirmed in pig until now. Therefore, this study was conducted to analyze their association with sperm motility and kinematic characteristics. DNA samples from 105 Duroc pigs with records of semen motility and kinematic characteristics [Total motile spermatozoa (MOT), Curvilinear velocity(VCL), Straight-line velocity(VSL), the ratio between VSL and VCL(LIN), Amplitude of Lateral Head displacement(ALH)] were analyzed. A SNP in coding region of ESR2 g.35547A > G in exon 5 was associated with MOT (p < 0.05) in Duroc population. Therefore, we suggest that the porcine ESR2 gene may be used as a molecular marker for Duroc boar semen quality, although its functional effects were not defined yet. These results might shed new light on the roles of ESR2 in spermatogenesis as candidate gene for boar fertility, but still the lack of association across populations should be considered.

Cluster-of-differentiation antigen 9 (CD9) gene expressed in the male germ line stem cells is crucial for sperm–egg fusion, and was therefore selected as candidate gene for boar semen motility and kinematic characteristics. This study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 96 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT), Curvilinear velocity(VCL), Straight-line velocity(VSL), the ratio between VSL and VCL(LIN), Amplitude of Lateral Head displacement(ALH)] were used in present study. A single nucleotide polymorphism (g.358A>T) in intron 6 was associated with MOT, VCL, VAP and ALH in Duroc population (p < 0.05). Therefore, we suggest that the porcine CD9 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the CD9 in spermatogenesis within the reproductive tracts, and will shed light on CD9 as a candidate gene in the selection of good sperm quality boars.

For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Estrogen receptors 2(ESR2) is involved in estrogen related apoptosis in cell cycle spermatogenesis, but their functions have not been confirmed in pig until now. Therefore, this study was conducted to analyze their association with sperm motility and kinematic characteristics. DNA samples from 105 Duroc pigs with records of semen motility and kinematic characteristics [Total motile spermatozoa (MOT), Curvilinear velocity(VCL), Straight-line velocity(VSL), the ratio between VSL and VCL(LIN), Amplitude of Lateral Head displacement(ALH)] were analyzed. A SNP in coding region of ESR2 g.35547A > G in exon 5 was associated with MOT (p < 0.05) in Duroc population. Therefore, we suggest that the porcine ESR2 gene may be used as a molecular marker for Duroc boar semen quality, although its functional effects were not defined yet. These results might shed new light on the roles of ESR2 in spermatogenesis as candidate gene for boar fertility, but still the lack of association across populations should be considered.

Cluster-of-differentiation antigen 9 (CD9) gene expressed in the male germ line stem cells is crucial for sperm–egg fusion, and was therefore selected as a candidate gene to investigate Duroc boar semen motility and kinematic characteristics. This study was performed to investigatetheir association with semen motility and kinematic characteristics. DNA samples from 96 Duroc pigs with records of sperm motility and kinematic characteristics [Total motile spermatozoa (MOT, 82.27±5.58), Curvilinear velocity(VCL, 68.37±14.58), Straight-line velocity(VSL, 29.06±6.58), the ratio between VSL and VCL(LIN, 47.36±8.42), Amplitude of Lateral Head displacement(ALH, 2.88±0.70)] were used in present study. A single nucleotide polymorphism (g.358A>T) in intron 6 was associated with MOT, VCL, VAP and ALH in Duroc population (p<0.05). Therefore, we suggest that the porcine CD9 may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not clear yet. These results will improve the understanding of the functions of the CD9 in spermatogenesis within the reproductive tracts, and will shed light on CD9 as a candidate gene in the selection of good sperm quality boars.

사료이용 측면에서 유지 및 성장에 필요한 섭취량과 체내에 이용되지 않은 섭취량으로 구분할 수 있 으며, 체내에 이용되지 않은 섭취량을 잔류사료섭취량(Residual Feed Intake; RFI)이라 한다. 본 실험 은 국내 종돈의 RFI 유전모수를 추정하기 위해 2001년부터 2014년까지 태어난 Duroc종 8,696두의 검 정자료를 활용하였다. 일당증체량과 RFI의 육종가 상관관계는 -0.2로 음의 상관으로 조사되었는데 (P>0.01), RFI를 낮추면 일당증체량 개량에도 긍정적인 영향을 줄 수 있는 것으로 사료된다. 회귀추정 방식에 따른 RFI1(일당증체량)과 RFI2(일당증체량, 등지방두께)의 유전력은 각각 0.37, 0.45로 고도의 유전력을 나타내었다. 향후 국내에서도 개체단위 사료섭취량 측정으로 추정된 RFI를 개량에 활용한다면 농가 수익 개선에 많은 도움을 줄 수 있을 것으로 판단된다.

The objective of this study was to estimate the effects of test seasons on backfat thickness and age at 90 kg body weight in Duroc pig populations. Data of a total of 40,228 Duroc pigs performance tested from 2005 to 2014 were used. The effects of sexes, years and seasons of the tests were significant (p<0.01) for the both traits. The least squares mean of the age at 90 kg body weight of the pigs tested in 2014 was significantly less than that of the pigs tested in the previous years. And the pigs tested in spring reached 90 kg body weight faster than the pigs tested in the other seasons. The least squares mean of backfat thickness of the pigs tested in autumn was thicker than that of the pigs tested in the other seasons. Pigs tested in spring had the thinnest backfat. There were seasonal variations in the least squares mean estimates of the age at 90 ㎏ both in male pigs (134.06 to 134.36 days), and in female pigs (139.47 to 139.65 days). Seasonal variations were also detected in least sqaures means of the backfat thicknesses in male pigs (11.31-11.34 ㎜) and in female pigs (13.05-13.07 ㎜). The simple and rank correlation coefficients between breeding values estimated using the trait values unadjusted for seasonal effects and those using the trait values pre-adjusted for seasonal effects trait values were all unity, for the both traits. These results indicate that the adjustment of the trait values with regards to seasonal variation had no effects on the estimates from genetic evaluations.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen Post-thawed in boar. Recently, polymorphism (g. 35756 T>C) of Estrogen Receptor 1 (ESR1) gene reported to be significant association with MOT. This study was conducted to evaluate the ESR1 gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.35756 T>C SNP of ESR1 was significantly associated with frozen semen motility and kinematic characteristics. The g.35756 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.001). The SNP was also significantly associated with ALH (P<0.05). Therefore, we suggest that the g. 35756 T>C polymorphism in the intron 1 region of the porcine ESR1 gene could potentially be applied in frozen semen programs to improve MOT trait, but only after validation in other populations.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.158T>C) of phospholipase C zeta (PLCz) gene reported to be significant association with MOT. This study was conducted to evaluate the PLCz gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.158 T>C SNP of PLCz was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.0001, p= 0.0002, p<0.0001 and p<0.0001, respectively). Therefore, we suggest that the intron region of the porcine PLCz, may be used as a molecular marker for Duroc boar post-thawed semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.358A>T) of cluster-of-differentiation antigen 9 (CD9) gene reported to be significant association with MOT. Also, CD9 gene was expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as candidate gene for boar semen. This study was conducted to evaluate the pig SNP (g.358A>T) of CD9 gene as a positional controlling for semen parameters of post-thawed boar semen. To results, the g.358A>T SNP of the CD9 gene was significantly associated with the traits such as MOT, curve linear velocity, straight line velocity, average path velocity and amplitude of lateral head displacement. Particularly, the g.358A>T SNP significantly has the highest association with MOT and animals with AA genotype (p<0.001). Therefore, we suggest that the g.358A>T in the intron 6 region of the porcine CD9 may be used as a molecular marker for Duroc boar Post-thawed semen quality, although its functional effect was not defined yet.

한국 재래돼지(KK) 69두 및 재래돼지 모돈과 듀록종 웅돈의 교배를 통하여 생산된 교잡종(KD) 88두 로부터 조사된 체중측정 기록에 대해 세 가지 비선형 성장곡선을 적용하여 KK(Korean Native Sow × Korean Native Boar) 및 KD(Korean Native Sow × Duroc Boar)의 성장모형을 추정하고, 추정된 성 장모형의 모수를 이용하여 KK와 KD에 대한 성장특성에 대한 기초자료를 제공하고자 실시하였다. 각각의 성장곡선 함수로 추정한 성장곡선은 다음과 같다. von Bertalanffy 모형의 경우 KK의 암컷, 수컷, KD의 암컷 및 수컷의 모형은 각각 , 및 로 추정되었다. Gompertz 모형의 경우 KK의 암컷, 수컷, KD의 암컷 및 수컷의 모형은 각각 ,, 로 추정되었다. Logistic 모형의 경우 KK의 암컷, 수컷, KD의 암컷 및 수컷의 모형은 각각 , , 및로 추정되었다. von Bertalanffy 모형의 경우 최대성장시기를 나타내는 변곡점()은 KK 암컷집단과 수컷집단에서 각각 4.40±0.12개월령 및 4.86±0.22개월령으로 추정되었고, KD 암컷 및 수컷집단에서는 각각 3.81±0.09개월령 및 4.00±0.08개월령으로 추정되었다. Gompertz 모형의 경우 는 KK암수 및 KD암수에 대해 각각 4.32±0.11개월령, 4.90±0.12개월령, 4.03±0.69개월령 및 4.11±0.06개월령으로 나타났다. Logistic 모형의 경우 는 KK암수 및 KD 암수에 대해 각각 4.18±0.07개월령, 4.41±0.05개 월령, 4.00±0.06개월령 및 4.03±0.04개월령으로 나타났다. 오차 평균 제곱합의 결과를 보면, KK의 성장은 Logistic, Gompertz 그리고 von Bertalanffy 모형 순으로 적합도가 좋은 것으로 판단되며, KD는 Gompertz, Logistic 그리고 von Bertalanffy 모형 순으로 적합도가 좋은 것으로 판단된다.

The objective of this study was to estimate genetic parameters on days to 90 kg body weight and ultrasound measured traits (back fat thickness, eye muscle area, retail cut percentage) and predict genetic progress when the weights for selection on the paternal traits of Duroc, which can be incorporated into sire line selection indexes. A simulation program, ZPLAN+, was used for the simulation. Genetic parameters of days to 90 kg body weight (D90KG), back fat thickness (BFT), eye muscle area (EMA) and retail cut percentage (RCP) used in the simulation were estimated from 27,972 heads of farm tested Duroc pigs from 2005 to July, 2012. Parameters of simulation used genetic parameters with parturition rates, ratios of test pigs and replacement rates that were dependent on selection protocols of simulated farms. Different weights of selection for traits of days to 90 kg and backfat thickness were compared. Estimates of heritability were 0.406, 0.355, 0.159 and 0.379 for D90KG, BFT, EMA and RCP, respectively. Genetic correlation of D90KG with BFT, EMA and RCP were –0.043, 0.049 and 0.021, respectively. Genetic correlation of BFT with EMA and RCP were –0.394 and –0.937. And genetic correlation between EMA with RCP were 0.524. Simulation result showed that index weight of 3:1 on D90KG and BFT was effective to shorten days to 90 kg without any significant changes in backfat thickness. Generally the genetic changes were greater as parturition rate and ratios of test pigs increased. Increase in replacement rate had a small effect on genetic changes in negative way. Thus, we recommend that index weight of 3:1 on days to 90 kg and backfat thickness could be utilized for sire line selection.

본 연구는 2004년부터 2011년까지 한국종축개량협회에서 듀록 품종에 대하여 수집된 40,657두의 검정기록 및 이들과 혈연관계가 있는 47,974의 가계혈통정보를 이용하여 산육형질에 대한 유전모수를 알아보고자 연구를 실시하였다. 듀록의 산육형질에 대한 유전모수 추정은 고정효과로써 성, 동기우 그룹, 산차 및 검정종료시 개체나이(공변이), 임의효과로써 개체의 상가적 유전효과 및 잔차효과를 포함한 혼합모형방정식을 구성하였으며, 분석은 리눅스 기반의 REMLf90 프로그램을 이용하여 다형질 분석을 실시하였다. 유전력은 90 kg 도달일령, 일당증체량, 등지방두께 및 등심단면적에서 각각 0.334, 0.340, 0.335 및 0.200로 추정되었다. 또한 형질간 유전상관의 결과를 살펴보면, 90 kg 도달일령과 일당증체량, 등지방두께 및 등심단면적에서의 유전상관이 각각 -0.992, 0.130, 0.358, 일당증체량과 등지방두께, 등심단면적에서의 유전상관은 각각 -0.142, -0.361, 등지방두께와 등심단면적간에는 -0.243로 나타났다. 90 kg 도달일령과 일당증체량간에 고도의 부의 상관을 보였으며, 90 kg 도달일령과 등지방두께, 등심단면적은 중저도의 정의 상관을 보였으며, 나머지 형질간에는 부의 상관을 보였다. 듀록 선발시 산육형질간 유전상관을 고려한 개량목표 설정이 필요할 것으로 사료된다.

본 연구는 후보 유전자의 경제형질에 미치는 염기 변이 효과를 검증하기 위해 국내에서 사육된 듀록 품종 96두와 한국 재래 돼지 86두를 활용하였다. 검증에 활용된 4개의 후보 유전자는 MC4R, PA-KAG3, FABP3 그리고 ESR 유전자였다. 각 후보 유전자들의 유전자형 분석 결과 두 집단 간에 유전적 특성의 차이가 분명히 나타나고 있음을 확인하였다. MC4R 유전자의 A 대립 유전자는 두 집단 모두에서 성장 형질과의 유의한 연관성이 검출되었고,

본 연구는 미니 돼지와 일반 돼지(Duroc)의 동결융해 후 정자의 수정 능력을 비교하여 동결 보존체계의 기틀을 확립하고자 하였다. 정액 제조는 수압법으로 정액 채취하여 1차 희석하였다. 동결은 LEY (1차: 11% -lactose+egg yolk, 2차: 1차 동결액+glycerol+OEP) 동결액을 이용하여 동결을 실시하여 동결 보존하였다. 동결 정액의 융해는 0.5 ml straw를 각각 20, 37 및 water bath에서 1분, 40 초 및