This study was conducted to determine the optimal dipping time and concentration of gibberellin for improving the growth and quality of domestic cultivar 'Seolhyang' strawberry when using runner plants. Strawberry runner plants were collected on November 10th and soaked in GA3 concentrations of 50, 100, and 150 mg·L-1 for 30 and 60 minutes, respectively. After 75 days of planting, the growth results showed that in the 30-minute, 50 mg·L-1 treatment, the crown diameter was thicker and the T/R ratio was lower, indicating better plant vitality. Runner length increased with lower gibberellin concentrations, particularly promoting vegetative growth. Photosynthetic efficiency was more influenced by gibberellin concentration than dipping time, and using concentrations above a certain threshold acted as a stress factor for runner plants, leading to decreased photosynthetic efficiency. For enhancing seedling growth, soaking with 50 mg·L-1 of gibberellin for 30 minutes was found to be optimal. This study verified the effects of gibberellin treatment on strawberry runner plants to improve plant growth and quality, providing useful basic data for using gibberellin.

농업 재배지 토양으로부터 auxin 생성세균 KSD16, KSD33 그리고 KSD36를 분리하였다. 분리 균주 KSD16, KSD33 그리고 KSD36 는 16S rRNA 유전자 계통분석을 통해 Arthrobacter 속으로 분류되 었다. 이들 균주는 R2A배지에 0.1% L-tryptophan를 첨가한 배지에서 28℃, 48 시간 배양한 결과, auxin 의 일종인 indole-3-acetic acid (IAA)를 204.4 mg L-1 생성하는 것으로 확인되었다. IAA 생성세균의 녹 두종자 발아에 미치는 영향을 확인한 결과, Arthrobacter 속 균주 KSD16, KSD33 그리고 KSD36 는 대조 군에 비해 뿌리길이와 발근수가 증가하였다. Arthrobacter 속 균주의 녹두 성장촉진 효과를 확인한 결과, 녹두의 발아율이 대조군보다 73.4 % 증가하는 특징을 나타내었다.

This study was conducted to investigate the effects of a powder of steamed and freeze-dried mature silkworms (SMSP), Bombyx mori, on hair growth/loss in vivo in C3H mice. Topically applied SMSP for at least 10 days or longer showed potent hair growth promoting activity. SMSP-treated mice showed increase in hair weight significantly compared with control mice which were not treated with SMSP. Orally administered SMSP also showed hair growth promoting activity, which was lower than that when applied topically. In conclusion, SMSP was identified as a potential candidate for a novel hair-loss preventing and/or hair-growth promoting agent, which shortened the telogen phase to advance the following anagen phase in C3H mice in vivo when SMSP was applied topically for at least 10 days or longer.

An auxin-producing bacterium Yangsong-1 was isolated from a button mushroom bed in Chung cheongnam-do. The strain Yangsong-1 was classified as a novel strain of Arthrobacter enclensis based on a chemotaxonomic and phylogenetic analysis. The isolated A. enclensis Yangsong-1 was confirmed to produce indole-3-acetic acid (IAA), which is one of the auxin hormones. When the concentration of IAA was assessed by HPLC quantity analysis, the maximum concentration of IAA, 152.903 mg L-1, was detected from the culture broth incubated in R2A medium containing 0.2% L-tryptophan for 48 h at 35oC. A negative relationship between IAA production and pH was estimated to show that the increase in IAA caused pH acidification of the culture. The effect of the supplement on L-tryptophan, a known precursor of IAA production, appeared to be at maximal production at 0.2% concentration and was rather reduced at concentration above 0.4%. To investigate the growth-promoting effects on the crops, the culture broth of A. enclensis Yangsong-1 was placed in water cultures and seed pots of mung beans and lettuce. In consequence, the adventitious root induction and root growth of mung beans and lettuce were 1.5 and 1.9 times higher, respectively, than those of the control.

충청남도 부여군 석성면 양송이 재배 농가에서 양송이 수확 후 배지로부터 토양을 채취하여 auxin(IAA) 생성능이 뛰어난 세균 Jopap-1 균주를 분리하였다. TLC 및 HPLC 분석을 통해 분리균이 생성한 IAA 농도를 확인한 결과, 0.1% L-tryptophan를 함유한 pH 7.0의 R2A broth 배지에 35℃, 48시간 배양 시 최대 생성농도는 96.05 mg L−1이었다. 생리적 특성 및 계통학적특성 분석을 통해 분리균은 Gram 음성 간균인 Klebsiella michiganensis Jopap-1로 동정되었다. 배양조건별 IAA의 생산능 비교 시, IAA 농도의 증가가 배양액의 pH 산성화에 기인함으로서 IAA 생성량과 pH 변화에는 부의 상관성이 있는 것으로 관측되었다. IAA 생성을 위한 전구물질로 알려진 L-tryptophan의 첨가효과는 0.1% 첨가 시 균의 생육 및 IAA 생성량이 최대이었으며, 0.2% 이상 고농도 첨가 배지에서는 오히려 IAA의 생성이 저해되었다. 또한 분리균에 의한 식물 생육 촉진 효과를 조사하기 위하여 수경재배 및 pot 재배를 통한 녹두발근 생검법과 상추발근 생검법을 수행한 결과, K. michiganensis Jopap-1의 배양액 접종 시 녹두발근 생검법에서는 대조구에 비해 발근수와 뿌리길이에서 약 2.1배의 뿌리 신장 효과를 보였고, 상추발근 생검법에서는 대조구에 비해 뿌리길이와 무게에서 약 1.8배의 뿌리신장효과를 보였다.

An auxin-producing bacterial strain, designated 4-3, was isolated from waste button mushroom compost in Boryeong-si, Chungnam. The strain 4-3 was classified as a novel strain of Leucobacter tardus, based on chemotaxonomic and phylogenetic analyses. TLC and HPLC the isolated L. tardus strain 4-3 produced indole-3-acetic acid (IAA), the auxin. Maximum IAA productionof 94.3 mg L-1 was detected for bacteria cultured in R2A medium with 0.1% l-tryptophan, incubated for 24 h at 35oC. Negative correlationwas observed between IAA production and pH of the culture medium, indicating that the increase inIAA caused acidification ofthe medium. The effect of supplementation with varying concentrations of l-tryptophan, a known precursor of IAA, was also assessed. production was maximal at 0.1% l, but decreased at lconcentrations above 0.2%. To investigate the plant growth-promoting effects of the bacterium, L. tardus strain 4-3 culture broth was used to inoculate water cultures and seed pots of mung bean. We found thatadventitious root induction and root growth were 2.2-times higher in thethan in the non-inoculated plants.

An auxin-producing bacteria (A-1) was isolated from soils of Oyster mushroom farmhouse in Daejeon city, South Korea. The strain A-1 was classified as a novel strain of Ochrobactrum anthropi based on a chemotaxanomic and phylogenetic analyses. The isolate was confirmed to produce indole-3-acetic acid (IAA), one of auxin hormones, by TLC and HPLC analyses. The maximum concentration of IAA, 5.6 mg L-1 was detected from the culture broth of O. anthropi A-1 incubated for 24 h at 35oC in R2A broth containing 0.1% L-tryptophan. To investigate the growth-promoting effects to the crops, the culture broth of O. anthropi A-1 was inoculated to water cultures and seed pots of mung bean as well as lettuce. In consequence, the adventitious root induction and root growth of mung bean and lettuce were 2.7 and 1.4 times higher than those of the non-inoculated, respectively.

큰느타리 (Pleurotus eryngii), 표고 (Lentinula edodes ), 노루궁뎅이 (Hericium erinaceus ) 버섯 수확 후 배지 (spent mushroom substrate, SMS) 물 추출액(SMSE)의 고추생장촉진과 고추역병균 억제효과를 조사하였다. 큰느타리버섯, 표고버섯 및 노루궁뎅이버섯 SMSE는 유묘생장시에 잎폭, 잎장, 초장, 절간, 절수 등에서 대조구에 비하여 모두 11~41%로 평균 20% 이상의 높은 생육효과가 있었으며 잎수에서는 거의 1.5배 높게 나타났다. 노루궁뎅이 SMSE혼합 PDA배지는 고추역병균사 성장을 거의 99-100% 억제하였다. 큰느타리, 표고버섯 및 노루궁뎅이 SMSE는 고추역병에 대하여 각각 25%, 65% 75%이상의 방제효과를 보였다.

The current study was conducted in order to investigate promotional effects of herbal extracts on hair growth in an animal model of mice. There were four experimental groups, including distilled water (DW) as a negative control (NC), 3% minoxidil (MXD) as a positive control (PC), 50% ethanol (EtOH) as a vehicle control (VC), and herbal extract (HE) as the experimental treatment (E). The HE was extracted with ethanol from plants, including Gardenia, Mentha arvensis, Rosemary, and Lavender. Six-week-old C57BL/6 male mice were shaved with an electric clipper and the test materials were topically treated with 0.2 ml per mouse daily for three weeks. Photographic evaluation of hair re-growth was performed weekly during a period of three weeks. The number of mast cells was counted on the dorsal skin section of mice. The enzymes, alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT), were determined using a biochemical autoanalyzer. No clinical signs were observed in any of the experimental groups. As a result of photometric analysis, topical application of HE to dorsal skin for two weeks resulted in significantly faster acceleration of hair regrowth, compared with that of the NC or VC group (P<0.05). The PC and E groups showed a significant decrease in mast cell population, compared to the NC group. Activities of ALP and γ-GT were significantly increased in the PC and E groups, compared to the NC or VC group (P<0.05). Taken together, these results suggest that the herbal extract may have hair-growth promoting activity equal to that of MXD.

This study was conducted in order to investigate the effect of black sesame oil on hair growth in a shaving animal model of C57BL/6 mice. Five-week-old male mice were acclimated for one week under 22±1 µl room temperature, 50±5% relative humidity, and 12 hours of a light/dark cycle before beginning the experiment. The animals were divided into three groups, including the normal group (saline, N), positive control group (3% MXD, PC), and experimental group (black sesame oil, E) and received topical application of 100 µl once per day, six days per week, for a period of three weeks. Hair regrowth was evaluated by gross and histological examination. In addition, immunohistochemical observation for SCF, the activities of enzymes, including ALP and γ-GT, and the expression quantity of growth factors, including IGF-1 in the skin of mice was performed or evaluated. Topical treatment with black sesame oil and 3% minoxidil for three weeks to dorsal skin resulted in more rapid acceleration of hair regrowth in the E and PC groups than in the N group. Development and elongation of hair follicles were promoted in the E and PC groups, compared with the N group. Serum ALP activity was significantly higher in the E group, compared with the N group within three weeks (p<0.05). Skin ALP activity was significantly higher in the PC group, compared with the N group within two weeks (p<0.05), and higher in the E and PC groups than in the N group within three weeks with no significant differences. Serum γ-GT activity was significantly higher in the PC group, compared with the N group within two weeks (p<0.01), and significantly higher in the E group, compared with the N group within three weeks (p<0.05). Skin γ-GT activity was significantly higher in the PC group, compared with the N group within two weeks (p<0.05), and significantly higher in the PC (p<0.01) and E (p<0.001) groups, compared with the N group within three weeks. IGF-1 expression in the skin was significantly higher in the PC and E groups, compared with the N group (p<0.01). SCF antigens were heavily stained in bulge, stem cells, and dermal papilla and middle stained in inner root sheath, outer root sheath, and epidermis in the E and PC groups. These results indicate that black sesame oil effectively stimulated hair growth in an animal model via several mechanisms and that it can be used practically for hair growth or prevention of hair loss in human beings.

오이를 재배했던 비닐하우스 토양에 병원균을 전면 접종하고 1983년 7월 25일부터 8월 25일까지 포리에칠렌 필림을 멀칭하여 태양열소독 효과를 실험하였다. 멀칭처리 기간중 토양의 일중 최고온도는 깊이 5cm에서 , 15cm는 , 25cm는 에 달했다. 태양열처리 한지 32일 경과후 지표로부터 5cm이내의 토양에 있던 병균은 완전히 사멸되었으며 15cm 까지는 이상 소멸되었으나 25cm 토양은 무처리와 차이가 없었다. 그러나 태양열처리한 토양에서 분리한 균주는 무처리 토양의 균주보다 소형포자 생산량이 현저하게 감소되었다. 멀칭처리에 재배한 오이는 생육이 월등히 좋았는데 특히 5cm 이내의 토양에서 두드러지게 나타났으며 노지재배의 경우도 생장과 수량이 무처리에 비하여 현저히 좋았다. 병원균 F. oxysporum f. sp. cucumerinum은 오이 유균에 외관상 나타나는 병징 이외에도 전염원의 농도가 증가됨에 따라 오이 생육이 비례하여 억제됨을 나타내었다.

The purpose of this study was to estimate the growth promoting effects and improvement of antioxidant activity of the soybean sprouts treated with Chlorella sp. culture solution. The soybean sprout treated with 0.1% and 0.2% Chlorella sp. culture solution was significantly increased the length (more than 43.0%), the thickness (more than 0.5~0.7 mm), fresh weight (more than 2.9~3.7 g) compared to non-treated control in vitro. In organic soybean sprouts farm, the 0.2% chlorella culture solution applied to mass culture of soybean sprout and the fresh weight of soybean sprouts increased by more than 25% and the yield was very high as 598.33% compared to untreated control. In addition of sensory test, there is no fishy odor and better crunchy texture and nutty flavor for the treatment soybean sprouts compared to untreated soybean sprouts. Particularly, free-radical scavenging activity (DPPH) and superoxide dismutase activity (SOD) of the soybean sprouts were significantly increased more than 26.1% and 40.4%, respectively by treated with 0.1% and 0.2% Chlorella culture solution. Consequently, the treatment of chlorella culture solution to grow soybean sprouts is also promoting quality and antioxidant activity as well as promoting the growth of sprouts. Therefore, chlorella is considered to be worth as functional materials for high-quality sprouts grown.

Endophytes are microorganisms that live in the internal tissues of plants without harming the host plants. In this symbiotic relationship, the host plants provide nutrients and shelter to the endophytes, in turn, endophytes can promote the growth of host plants and act as a biological control agents against plant pathogens. Plant-microbe interactions like this are noted for natural methods for sustainable agriculture and environmental conservation. However, in spite of the infinite potential, there are only a few reports on the endophytes present in ginseng. In this study, we isolated and identified the endophytes from Panax ginseng seeds and evaluated the biological activities (IAA production ability, nitrogen fixation ability, phosphate solubilization capacity, siderophore production ability, and antifungal activities) of the endophyte isolates. Eight different endophytes were identified by 16S rRNA sequencing. Most of the endophytes have antibiotic and plant growth promoting (PGP) activities. Particularly, PgSEB5-37E have the highest antibiotic activity, both PgSEB5-37B and PgSEB5-37H have high PGP traits such as an abilities to produce IAA, solubilize phosphate and fix nitrogen. These results indicated that the endophytes from P. ginseng seeds may have applicable value to many industries. In order to use the isolated endophytes, quantitative analysis and field tests are needed to be performed.

최근 들어 홍삼의 발모효과가 실험적으로 입증이 되었으나 임상적인 효능에 대해서는 아직 연구가 부족한 실정이다. 본 임상시험에서는 홍삼사포닌 Rg3 0.003%가 함유된 샴푸(Somang Co., Korea)의 사용에 따른 탈모방지 및 발모 탈모 및 발모효과를 인체적용시험을 통해 확인하고자 한다. 탈모로 진단된 42명의 환자가 참여하였으며, 홍삼사포닌 Rg3 0.003%가 함유된 헤어샴푸를 사용하는 군과 홍삼사포닌 Rg3 0.003%를 제외한 헤어샴푸를 사용하는 군으로 나누어 16주간 제품 사용 후 모발의 굵기, 밀도, 성장속도를 측정하였고, 탈모 개선 정도의 전문가 육안평가 및 피험자의 주관적 만족도로 평가하였다. 또한 제품사용의 피험자 주관적 기호도를 추가적으로 조사하였다. 시험 결과 시험제품 사용 후 16주에서 모발의 굵기, 모발의 밀도 및 모발 성장속도가 모두 통계적으로 유의성 있게 증가하였으며 전문가 육안평가와 피험자의 주관적 설문평가, 환자의 기호도 면에서도 시험제품이 더 우수한 결과를 보였다. 결론적으로 홍삼사포닌 Rg3가 함유된 샴푸는 피부에 자극 없이 탈모방지 및 양모개선 효과에 도움을 주는 제품으로 판단된다.

In this study, to develop hair growth agents using natural products which has excellent ability to promote hair growth effect and fewer side effect, animal experiment and clinical trials was performed to evaluate hair growth promotion effect of herbal product containing Rosa mutiflora roots extracts as a main component (RMHP). 4-week-old male C57BL/6 mice were removed the dorsal hair using thioglycolate, and applied 15% EtOH solution as a negative control, 5% minoxidil as a positive control and RMHP to dorsal skin. In the results of macroscopy and photo-interpretation, RMHP group recorded 100% (+++++) of hair growth was proved to significantly stimulate hair growth against 80% (++++) negative control group. 37 patients were treated with RMHP and evaluated the therapeutic effect at 16 weeks. Hair density was significantly increased at 16 weeks after applying RMHP (125.0±4.9 FU/cm2) compared to before treatment (104.3±4.7 FU/cm2, p 〈 0.05), and hair thickness were also significantly increased (0.066±0.003 mm) than before treatment (0.055±0.002 mm, p 〈 0.05). The result of clinical photo-interpretation using 7-point rating scale assessment, after 16 weeks clinical symptoms were evaluated to significantly improve with 1.23±0.05 (p 〈 0.05). Therefore, the results of this study were observed that RMHP have hair loss prevention effect and hair growth promotion effect to hair loss patients.

Endophytic Aspergillus Fumigatus sp. LH02, isolated from the roots of soybean and indentified through phylogenetic analysis of 18S rDNA sequence, was found stimulatory to the growth of Waito-C rice seedlings among 13 other endophytic strains screened. In growth hormone analysis, pure culture of fungal isolate produced three gibberellins viz GA4, GA9 and GA12. To know the role and effect on soybean in endophytic association (EA) and non-endophytic association (NEA) under salt stress (70 and 140mM), experiment was carried out at growth chamber using complete randomized block design. The results elaborated that the EA has significantly increased the shoot length, shoot and root fresh and dry weights, chlorophyll content, photosynthesis rate and leaf area of the plants with and without salt stress compared to control. Field emission scanning electron microscope analysis of root's surface showed that EA plants were extensively colnized by Aspergillus fumigatus LH02 under high salanity. In phytohormonal anlysis, ABA contents in EA and NEA were almost similar. Howere, when plants were exposed th salt stress, ABA levels were found significantly low in EA compared to NEA plant. In isoflavone content analysis, aglycones, malonyl, acetyl and glucosides isoflavones (total 11 compounds) were analyzed for each treatment through extensive HPLC andalysis. The quantities of isoflavone were found higher in EA plant's leaves with and without salt stress compared to NEA plant leaves. In conclusion, such endophytic association can be beneficial to explore ways to improve crop production under stressful conditions.