This study investigated the surface tension and foaming properties of the hot-water extracts of pumpkin leaf and chickpea, as well as the effects of the plant hot-water extracts on white pan bread baking. Propylene glycol alginate (PGA), a synthetic emulsifier widely used in bakery, was used as a control. Pumpkin leaf water extract showed lower surface tension and comparable foaming capacity, compared with chickpea water extract and PGA solution when total solid  0.15% (w/w). Chickpea water extract showed the highest foam stability when total solid  0.15% (w/w). The dough was found to have a weak gel structure, and its viscoelastic properties were not significantly influenced by adding 0.05% or 0.15% (w/w) (based on total solid content) plant water extracts or PGA. The specific volume of the bread increased, and the baking loss was reduced by adding the two plant water extracts of total solid 0.15% (w/w). The hardness and chewiness of the bread crumb were reduced to a level comparable to the crumb containing 0.05% (w/w) PGA. The results showed that the pumpkin leaf water extract could be an effective natural emulsifier with a high phenolic content for bakery products.

Protaetia brevitarsis seulensis larvae from industrial insects are traditionally recognized as functional health foods in South Korea. We evaluated the immuno-modulatory effects of feeding beneficial microorganism (Bacillus velezensis TJS119) to P. brevitarsis larvae as a dietary source. In this study, we investigated the immune-enhancing activities of P. brevitarsis larvae hot-water extract (PLW) and PLW after treatment with B. velezensis TJS119 (PLWB) using the RAW 264.7 macrophage cell line. We examined the effects of PLWB on cell proliferation, cytokine production, and nitric oxide production in RAW264.7 cells. PLWB showed no cytotoxicity at concentrations ranging from 7.8 to 1,000 μg/mL in RAW264.7 cells. Treatment with PLWB increased the production of nitric oxide and pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β)] at doses of 62.5 to 1,000 μ g/mL in RAW264.7 cells. As a result, PLWB exhibited a stronger immune-enhancing effect compared to PLW. In conclusion, the results of this study offer experimental evidence to support the potential utilization of PLWB as an immunity-enhancing nutraceutical ingredient.

최근 들어 반려동물을 키우는 가구 수가 증가하고 있다. 그로인해 진드기와 냄새에 대한 관심 또한 증가하고 있다. 본 연구는 제충국 추출물 (Tanacetum cinerariifolium), 수용성 피톤치드(water-soluble phytoncide)가 진드기 퇴치제로서 효과와 탈취 및 항균 효과가 있는지 알아보기 위하 여 연구를 진행하였다. 틱 클라이밍 테스트(Tick climbing test)에서 제충국 추출물(Tanacetum cinerariifolium) 23.33점, 수용성 피톤치드 22.00점 으로 높은 기피효과를 보였다. 복합추출물로서의 진드기 기피 효과를 확인하기 위해 위 실험에서 기피 효과가 확인된 제충국 추출물, 수용성 피톤치 드를 포함한 복합추출물(Sample이라 함.), 타사 제품(Control이라 함.)로 틱 클라이밍 테스트(Tick climbing test)를 진행하였다. 결과 Sample 13.00점, Control 26.67점으로 Sample이 높은 기피 효과를 나타내었다. 참진드기 실내 기피제 효력 시험법에서도 Sample이 88%, Control이 12%로 우수한 진드기 기피효과를 보인다는 것을 확인하였다. Sample을 가지고 탈취 시험을 진행한 결과 암모니아에서 98.3%, 트리메틸아민에서 99.5%의 탈취 효과를 보여주었으며, 항균시험에서 대장균, 황색포도상구균, 폐렴균 모두 99.9%의 항균 효과를 보여주었다. 본 연구결과를 종합해 볼 때 위 소재를 포함한 복합추출물은 추후 진드기 기피제와 탈취 및 항균에도 효과가 있는 소재로서의 활용 가치가 높다고 판단된다.

Nypa fruticans Wurmb (NFW) contains a large amount of phenolic acid and flavonoids, and is popular as a superfood in Myanmar. NFW has various biological activities, such as anti-inflammatory, anti-oxidant, and neuroprotective properties; however, the anti-cancer effect of NFW have not been reported. In this study, we investigated the anticancer activity of water extracts of NFW (WeNFW) and the underlying mechanism in human FaDu hypopharyngeal squamous carcinoma cells. The WeNFW inhibited FaDu cell growth in a dose-dependent manner without affecting normal cells (L929), as determined by an MTT assay and Live and Dead assay. In addition, the concentrations of WeNFW without cytotoxicity (0.025, 0.05, and 0.1 mg/mL) inhibited wound healing and colony formation. Furthermore, WeNFW significantly induced apoptosis through the proteolytic cleavage of caspase-3 and -9, poly (ADP-ribose) polymerase, and downregulation of Bcl-2 and upregulation of Bax in FaDu cells, as determined by DAPI staining, FACS analysis, and western blot analysis. Taken together, these results suggest that WeNFW exhibits potent anti-cancer effects by suppressing the growth of oral cancer cells, wound healing and colony formation activity. Via mitrochondrial-dependent apoptotic pathways in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, WeNFW can provide a natural chemotherapeutic drug for oral cancer in humans.

Pumpkin sweet potato (Ipomoea batas L.) has been known as a traditional remedy and food source, not only in South Korea but worldwide. It is rich in fiber, potassium, vitamin C, and other minerals and vitamins, making it a nutritional food loved by many. showed that pumpkin sweet potato had antioxidant biological effects. The in vitro study showed that both splenocytes and cytokine production byactivated peritoneal macrophages increased when water extracts were supplemented at 100 and 250 μL/mL. Notably, the production of IL-1β, TNF-α, and IFN-γ by splenocytes was significantly increased at 100 μL/mL. The results suggest that supplementation with pumpkin sweet potato (Ipomoea batas L.) water extract may enhance immune function by stimulating splenocyte proliferation and improving cytokine production, activating macrophages in vitro.

The purpose of this study was to investigate the antioxidant activity and β-glucan content of extracts extracted by varying the temperature at 30, 55 and 80℃ after hot air drying or freeze drying of Pleurotus ostreatus and Pleurotus eryngii. For the analysis antioxidant activity of each mushroom, β-glucan, total phenol, flavonoid contents, and DPPH․ABTS+․Nitrite assay were measured. Also, the β-glucan content, total flavonoid content and ABTS+ scavenging activity increased with freeze drying rather than hot air drying, and increased with increasing extraction temperature in both mushrooms. However, the total phenol and nitrite scavenging activity increased with hot air drying rather than freeze drying, and decreased with increasing temperature in both mushrooms. DPPH scavenging activity was not significant in both mushrooms, but decreased with increasing extraction temperature. Pearson’s correlations between total flavonoid content and antioxidant activities were r=0.719~0.753 (p<0.01). As a result, the β-glucan content, total flavonoid content, and ABTS radical cation scavenging activity were highest during freeze drying and extraction at 80℃. And the highest total phenol content, DPPH radical scavenging activity and nitrite scavenging activity were obtained during hot air drying and extraction at 30℃.

The purpose of this study was to examine the physiochemical characteristics of hot water extract of Caragana sinica roots and verified its protective effect on MIA-induced osteoarthritis in rats. The contents of total polyphenol and total flavonoid used in this experiment were 0.40 mg/g and 0.22 mg/g respectively. We found that the weight of all experiment groups which ingested Caragana sinica extract for three weeks evenly increased in rats with MIA-induced osteoarthritis without toxicity in the liver or kidney. In the histopathological test through the Mankin score, the extent of damage of knee joint tissue in the experiment groups to which Caragana sinica extract was administered in 5 mL/kg or 10 mL/kg was significantly lower than that of the negative control groups statistically(p<0.05). As for the grade of osteoarthritis, the extent of tissue damage of the experiment groups to which Caragana sinica extract was administered in 5 mL/kg or 10 mL/kg was lower than that of the negative control groups as well.

본 연구에서는 고구마(Ipomoea batatas L.) 수확 후 버려지는 잔재물의 활용가치를 위해 이들로부터 추출한 발효 추출물과 열수 추출물의 생리활성과 세포독성을 분석 하였다. 추출물의 pH는 모두 산성을 나타내었고, 유기물 함량은 발효 추출물과 열수 추출물에서 각각 0.98%와 0.97%로 비슷하게 나타내었다. 다량원소 중 인산, 칼륨, 칼슘, 마그네슘 성분의 함량은 열수 추출물에서 발효 추출물보다 모두 높게 나왔고, 질소 함량만 두 추출물에서 동일하게 나왔다. 미량원소 함량은 아연을 제외하고 발효 추출물보다 열수 추출물에서 높게 나타내었다. 총 폴리페놀 함량은 열수 추출물에서 60.5±2.7 mg/g로서 발효 추출물의 총 폴리페놀 함량의 22.7±4.2 mg/g 보다 37.8 mg/g 높은 함량을 나타내었다(p<0.05). 총 플라보노이드 함량은 열수 추출물에서 50.7±2.7 mg/g로서 발효 추출물의 함량인 14.0±2.1 mg/g 보다 36.7 mg/g 높은 함량을 나타내어 총 폴리페놀 함량과 마찬가지로 열수 추출물이 발효 추출물보다 높은 함량을 나타내었다(p<0.05). DPPH와 ABTS radical 소거능력은 모두 열수 추출물에서 발효 추출물 보다 높은 항산화력을 보였다(p<0.05). MTT assay를 이용한 추출물의 세포독성 실험에서는 두 추출물의 모든 농도에서 세포독성이 미약한 것으로 확인되었다. 따라서 고구마 수확 후 잔재물의 추출물이 향후 각종 바이오 소재로 이용 시에도 큰 문제가 없을 것이라 판단된다.

Nelumbo nucifera Gaertn has been usedas a traditional remedy and food source in South Korea. It promotes gastrointestinal function and controls blood pressures. Nelumbo nucifera Gaertn water extracts supplement at 5, 10, 50, 100, 250, 500, 1,000 μg/mL after a 48 h pre-treatment with the mitogen (ConA or LPS) increased the mouse splenocytes proliferation. Water extract supplement also increased the cytokine production (IL-1β, TNF-α and IFN-γ), measured by a cytokine ELISA kit. For the result of in vitro study, the proliferation of splenocytes and cytokine production activated by peritoneal macrophages increased when water extracts were supplemented in the range of 50~500 μL/mL concentration. Specifically, the levels of the splenocytes proliferation, IL-1β, TNF-α and IFN-γ were the highest at 250 μL/mL concentration. This in vitro study suggestedthat supplementation with Nelumbo nucifera Gaertn water extracts may enhance the immune function by regulating the splenocyte proliferation and enhancing the cytokine production activating macrophage in vitro.

본 연구는 박하 추출물을 통해 독성검사 및 항산화, 항염증과 같은 생리활성을 평가하고자 하였다. 박하 추출은 열수 및 80% 에탄올로 추출하였으며, RAW 264.7 세포를 통해 MTT를 통해 세포 생존율을 평가하였다. 또한, LPS로 유도한 RAW 264.7 세포를 통해 활성산소(ROS), 산화질소(NO), 류코트리엔 B4(LTB4), 항염 또는 염증성 사이토카인(IL-1β, IL-6, TNF-α, IL-10) 등을 ELISA 및 Luminex로 측정하였다. 그 결과, 박하 추출물은 열수 및 80% 에탄올 추출물에서 세포독성이 없었으며, 80% 에탄올 추출물의 100 ㎍/㎖ 농도에서 활성산소의 생성을 억제하였다. 또한, NO, LTB4, 염증 또는 항염증성 사이토카인의 생성을 모든 추출물에서 농도 의존적 효능을 나타냈다. 즉, 박하 추출물은 세포 독성 없이 항산화 및 항염증 활성을 나타내는 생리학적 효능이 나타났다. 이와 같은 결과는 본 연구와 관련된 증상을 개선하기 위한 새로운 건강식품 및 치료제의 원료로 개발될 수 있다.

The Sasa quelpaertensis Nakai is a white band on the edge of the leaf, the branch is not split, the stem is small, and the shape of the node is different from that of the inland bamboo (Sasa borealis Makino). It is known as a plant that is highly likely to be used as a resource plant. Various efficacy studies such as antioxidant, anti-obesity, antibacterial and antidiabetic activities have been conducted and developed as functional cosmetics and beverage products. Unfortunately there is little research on the suitability of the product. In order to use S. quelpaertensis as a raw material for food or health functional food, S. quelpaertensis leaves were collected monthly for one year to prepared 70% ethanol extract(SQEP) and water extract(SQWP). After, the content of index component(p-coumaric acid) in SQEP and SQWP was analyzed by HPLC. For the study of the processing properties, the SQWP was prepared considering the content of the index component and extraction yield(%). Also, the stability of index component according to pH(2.5, 4.0, 6.0, 8.0, 10.0) condition and stability of index component according to temperature condition(25℃, 35℃, 40℃) were studied. Through this process ability(properties) study, we suggest that the SQWP may be used as a product material of health functional foods.

치자 씨의 에틸 아세테이트, 증류수 및 70% 메탄올(v/v)의 3가지 용매를 사용한 추출물의 용매 별 플라보노이드 함량 및 항산화 활성, 금속 킬레이트 능력 측정을 통하여 치자의 기능성 식품 재료로서의 가치를 검토하기 위하여 본 실험을 수행하였다. 치자 씨의 탄닌 함량은 1.517±0.003 mg CE/g (mg of catechin equivalents) 건조 중량으로 나타났으며, 치자 씨 용매 별 추출 수율은 증류수 (36.61%), 70% 메탄올(30.10%), 에틸아세테이트(20.40%) 순으로 나타났다. 추출 용매 별 항산화 활성은 농도(0.2, 0.4, 0.6 mg/mL)가 증가할수록 유의적으로 증가하였으며(p<0.05), 양성대조구로 사용된 ascorbic acid, BHA, EDTA 보다 낮은 활성이 관찰되었다. 플라보노이드 함량(mg QE/g, mg of quercetin equivalents)은 70% 메탄올(0.799), 증류수(0.565), 에틸아세테이트(0.117) 순으로 관찰되었으며, 항산화 활성은 대부분의 분석에서 용매 별 플라보노이드 함량 순과 비슷한 양상으로 나타났지만 ABTS 라디칼 소거능과 SOD 유사활성은 증류수 추출물이 70% 메탄올 추출물의 항산화 활성 보다 높은 것으로 나타났다. 치자 씨의 각 용매별 수율과 항산화 활성을 비교하였을 때, 증류수와 70% 메탄올 용매를 사용하여 추출 이용하는 것이 바람직하다고 생각된다. 본 실험 결과 치자 씨는 플라보노이드 화합물을 다량 함유하고 있으며 그로 인한 높은 수준의 항산화 활성과 생리활성을 가지고 있어 기능성 식품 및 천연 항산화제로서의 가치가 매우 기대된다.

Caesalpinia sappan L. is an oriental medicinal plant distributed in the Asia Pacific region including India, Malaysia, and China. The dried heartwood of Caesalpinia sappan has been traditionally used as an analgesic and anti-inflammatory drug. In this study, the effects of extract methods of C. sappan on contents of total polyphenols and flavonoids, antioxidant activity, and cytotoxic activity were evaluated. As a result, hot water extract from C. sappan (CSWE) significantly exhibited contents of total polyphenols and flavonoids (22.6 mg GAE/g and 14.5 mg QE/g) higher than 70% ethanol extract (CSEE) (17.6 mg GAE/g and 13.2 mg QE/g). However, CSEE showed greater antioxidant activity than CSWE in both DPPH and ABTS. Also, the cytotoxicity of C. sappan against three kinds of cancer cell lines was higher in CSEE than in CSWE. These results show that ethanol extract is a better extract method than hot water method to maintain antioxidant and anti-cancer activities.

Anthriscus sylvestris (L.) Hoffm. is a perennial herb found widely distributed in various regions of Korea, Europe, and New Zealand. The root of A. sylvestris have been extensively used in the treatment for antitussive, antipyretic, cough remedy in Oriental medicine, but the physiologically active function of the leaf of A. sylvestris is as yet unknown. In this study, we investigated the anti-cancer activity and the mechanism of cell death of water extracts of leaf of Anthriscus sylvestris (WELAS), on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that WELAS treatment inhibited cell viability in a concentration- and time-dependent manner. In addition, the treatment of WELAS markedly induced apoptosis in FaDu cells, as determined by the viability assay, DAPI stain and FACS analysis. WELAS also increased the proteolytic cleavage of procaspase-3, -9 and PARP (poly(ADP-ribose) polymerase). In addition, exposure to WELAS decreased the expression of Bcl-2 (an anti-apoptotic factor), but increased the expression of Bax (a pro-apoptotic factor), suggesting that mitochondria-dependent apoptotic pathways are mediated in WELAS-induced apoptosis. Taken together, these results indicate that water extracts of leaf of A. sylvestris inhibits cell growth and induces apoptosis via the mitochondrial-dependent apoptotic pathway in FaDu human hypopharyngeal squamous carcinoma cells. Therefore, we propose that the water extracts of leaf of A. sylvestris is a novel chemotherapeutic drug, having growth inhibitory properties and induction of apoptosis in human oral cancer cells.

Plantago asiatica L., observed frequently in East Asia, is a known herb used in traditional medical remedies several studies report that P. asiatica L has anti-inflammatory and antioxidant effects. To determine the production of cytokines (IL-2, IFN-γ, and TNF-α) induced by lipopolysaccharide (LPS) and non-LPS-stimulated macrophages, an ELISA assay was conducted using cytokine kits. Mice splenocytes were cultured for 48 h with various concentrations of P. asiatica L. (5, 10, 50, 100, 250, 500, and 1,000 μg/mL) or with mitogens (ConA or LPS). P. asiatica L. increased the proliferation of mice splenocytes, especially under the condition of its concentration ranging from 250 to 1,000 μg/mL. In addition, Plantago asiatica L. notably induced cytokine production of (IL-2, IFN-γ, and TNF-α) at its concentration of 250~500 μg/mL. These results suggest that supplementation with P. asiatica L. water extracts may play a potential role in enhancing immune function by mediating splenocyte proliferation and cytokine production through its anti-inflammatory activit.

본 연구에서는 뱀딸기풀의 열수추출물과 에탄올추출물간의 항산화 활성 비교를 위해 25~500μg/mL의 뱀딸기풀의 농도를 이용하여 in vitro 실험으로 관찰하였다. DPPH radical 소거능은 열수추출물 (16.23~89.54%)과 에탄올추출물(6.85~89.41%)에서 농도 의존적으로 높은 활성을 나타내었으며, ABTS radical 소거능을 비교한 결과는 열수추출물에서 15.48~93.49%, 에탄올추출물에서는 7.42~92.81%로 농도 의존적으로 높은 소거 활성을 나타내었다. 이 두 결과를 미루어 열수추출물은 에탄올추출물에 비해 유의적 으로 더 높은 radical 소거능을 나타내는 것을 확인할 수 있었다. 환원력은 열수추출물에서 0.167~1.023, 에탄올 추출물에서 0.161~0.783의 활성을 나타내었다. NO radical 소거능은 열수추출물에서 16.24~ 67.93%, 에탄올추출물에서 12.46~70.78%의 활성을 나타내었으며 특히 75~200μg/mL 농도에서는 열수추 출물이 에탄올추출물에 비해 유의적으로 더 높은 활성을 나타내었다. 뱀딸기풀 추출물의 IC50값을 비교 한 결과 DPPH, ABTS radical 및 NO radical에서 각각 열수추출물에서 90.6μg/mL, 101.3μg/mL 및 147.0μg/mL으로 나타났고, 에탄올추출물의 경우에는 164.7μg/mL, 151.9μg/mL 및 235.6μg/mL으로 나타 났다. DPPH, ABTS radical 및 NO radical 모두에서 에탄올추출물이 더 높은 IC50값을 보였다. 총 페놀 함량은 500μg/mL, 1000μg/mL의 농도에서 열수추출물에서 각각 42.3mg/g, 91.6mg/g, 에탄올추출물에 서 39.6mg/g, 82.3mg/g의 함량을 나타내었다. 플라보노이드 함량에서는 500μg/mL, 1000μg/mL의 농 도에서 열수추출물에서 각각 26.3mg/g, 31.9mg/g, 에탄올추출물에서 22.3mg/g, 27.7mg/g의 함량으로 열수추출물이 유의적으로 더 높은 함량을 나타내었다. 이러한 결과로 미루어 뱀딸기풀의 용매에 따른 추 출물은 항산화의 효과를 나타내는 실험 모두에서 높은 활성을 나타내었으므로 뱀딸기풀은 효과적인 항산 화 효능을 가진 천연식품으로 이용되어 질 수 있으리라 기대되어진다.