본 연구는 사과 ‘홍로’의 생육 시기별 품질 변화를 분석하여 숙기를 진단하기 위한 최적 수확 지표를 선정하고, 기계 수확 이 가능한 사과 ‘홍로’의 품질 기준을 설정하고자 수행되었다. 전라북도 완주군에 위치한 국립원예특작과학원에서 재배된 7년생 사과 ‘홍로’ 품종을 대상으로 2021년부터 2023년까지 만개 후 58－59일(6월 상순)부터 수확 시기가 지난 만개 후 164－173일(9월 하순)까지 10일 간격으로 과실 품질을 조사 하였다. 또한 완주군, 화성시, 포천시에서 수집된 품질 데이터 를 활용하여 지역별 ‘홍로’ 품질 변화를 분석하였다. 과중은 생육이 진전됨에 따라 증가하다가 수확기 직전 250－320g 범 위에 도달했다. 경도와 산도는 생육이 진전되면서 감소했고, 가용성 고형물 함량은 증가하다가 12－14°Brix 에서 더 이상 증가하지 않는 경향을 나타냈다. 또한 당산비는 생육이 진전 될수록 증가하여 수확기에는 50 이상으로 증가했다. 색도 L* 값과 b*값은 증가하다가 수확기에는 급격하게 감소했으며, a* 값은 수확기에 급격히 증가하였다. 전분 함량은 생육 후반부 에 급격히 감소하여 수확기에는 20mg·g-1 이하로 낮아졌다. 전분 함량과 과실 특성들 간의 상관분석을 한 결과, 경도와 산도가 각각 0.86과 0.84로 높은 상관성을 나타냈다. 따라서 경 도와 산도를 최적 수확 지표로 선정하였으며, 수확 가능 최소 품질 범위는 경도 39.7±6.4N, 산도 0.255±0.052%, 가용성 고 형물 함량 13.6±1.3°Brix, 색도 a*값 14.7±12.8로 설정하였 다. 이 기준과 함께 회귀 계수가 높은 비파괴 조사항목인 종경, 횡경, 색도a*값을 활용한다면 기계 수확뿐만 아니라 재배자 에게도 유용한 정보를 제공하여 수확 시기를 최적화시킬 수 있을 것으로 기대한다.

This study evaluated the applicability of existing overseas-developed allometric equations for estimating the biomass of domestic apple trees (Malus domestica) in South Korea. To assess their suitability, predicted biomass values derived from these equations were compared with actual measurements obtained through destructive sampling. The results indicate that some overseas equations showed no significant difference when applied to domestic apple trees, suggesting their potential applicability to local conditions. However, it was also noted that certain equations may not fully capture the unique characteristics of domestic apple trees, such as the slender spindle form associated with modern high-density planting practices. Given these findings, the study highlights the need to develop new allometric equations that are specifically optimized for the growth characteristics and cultivation environments of domestic apple orchards. Such equations would enable more accurate biomass estimation, support agricultural sustainability, and provide reliable data for the national greenhouse gas inventory. This research is expected to serve as a foundation for future Tier 2-level studies on biomass and carbon sequestration in apple orchards while also underscoring the importance of expanding research to include various fruit tree species to develop customized biomass estimation models for enhanced agricultural management and environmental conservation.

본 연구는 국내육성 사과품종들 중 대구광역시 군위지역에 재배가 적합한 품종을 선발하고자 ‘골든볼’, ‘아리원’, ‘아리수’, ‘이지플’, ‘피크닉’, ‘컬러플’사과품종에 대하여 과실특성 및 저장성을 분석하였다. 각 품종에 대해 품종등록시 과실특성과 비교하였을 때, ‘골든볼’사과는 수확시 경도가 88.4 N으로 다른 품종들에 비하여 매우 높고, 당도와 산도가 각각 14.4 oBrix와 0.37%를 보였으며, 과피색의 황색도는 44.1로 황색계통의 특성이 충분히 발현되었다. 그리고 ‘피크닉’사과는 경도가 66.2 N, 당도 14.7 oBrix, 산도 0.33%를 보였고, 과피 양광면의 적색도는 29.9로 적색계통의 특성이 잘 나타났으나, 바탕면의 적색도는 2.5로 낮아 과실 전체의 착색이 부족하였다. 그리고 ‘아리원’, ‘아리수’, ‘이지플’, ‘컬러플’과실들은 품종등록시 과실특성 기준에 비하여 당도와 산도가 모두 낮은 결과를 보였다. 저온저장중 과실품질변화를 보면, 경도는 6품종 모두 수확시와 비슷한 수준을 유지하였다. 특히, ‘골든볼’ 사과는 저장 80일 후에도 경도가 79.7 N으로 높게 유지되고, 당도와 산도, 그리고 당산비는 수확 시와 비슷하게 유지되었다. 저온저장중 과피색 변화는 품종과 관계없이 명도는 유지되고 적색도와 황색도는 저장기간에 따라 다소 증가하는 경향을 나타내었다. 따라서 대구광역시 군위지역에서는 과실의 유전적 특성이 잘 발현되는 ‘골든볼’사과품종의 재배가 가장 적합할 것이라고 판단되었다.

Background: Typical difficulties encountered during in vitro fertilization (IVF) to produce embryos in pigs include poor pronucleus formation and poor-quality fertilized embryos because of high polysperm invasion. In this study, we evaluated the effects of supplementation with apple seed extract (ASE) and coculture systems on porcine in vitro-fertilized embryo culture. Methods: Slaughterhouse-derived ovaries were used to obtain cumulus-oocyte complexes (COCs). COCs were conventionally used to perform IVF. We examined the differences in apoptosis and metabolism during development following addition of ASE to normal culture and coculture systems. Matrix metalloproteinases (MMPs), cell development-related factors, and apoptotic proteins were compared in porcine embryos produced under different conditions. Results: The expression of genes related to insulin-like growth factor (IGF) signaling was increased in the coculture system. In the ASE group, early apoptosis and necrosis were reduced in fertilized embryos and the late survival rate increased. Supplementation of the coculture system with ASE led to increased expression of BCL-2 and decreased expression of Casp-3 in the cytoplasm, thereby lowering the apoptosis rate and inducing MMP expression. In addition, compared with the extract-supplemented group in normal culture, the activity of MMP-2 decreased in the coculture system supplemented with ASE, activity of MMP-9 increased, and the expression of dynactin p62 and BrdU in the cytoplasm was higher than that in the other groups. Conclusions: The coculture system increased the activity of the embryonic cytoplasm compared with the non-coculture system. Supplementation with ASE may induce cell activity and inhibit the expression of apoptotic factors.

To develop farm-made high effective vinegar, this study prepared apple vinegar using four kinds of acetic acid bacteria isolated from a natural fermentation liquid of ‘Cheongsoo’ grapes and analyzed vinegar samples fermented 93% apple juice and 7% alcohols at 30℃ for 20 days. To accomplish this, quality characteristics such as pH, total acidity, reducing sugar, organic acid, color, total polyphenol contents, and antioxidant activity contents were determined. The pH decreased while total acidity of all samples gradually increased during fermentation period. The vinegar with AP 21 strain tended to increase the total acidity quicker than other stains. Reduced sugar content remained high until the last fermentation day. Furthermore, reduced sugar contents of all vinegars increased as fermentation progressed by decomposing sucrose present in apples. When physiological activities were compared, apple vinegars fermented with AP 21 and 30 strains had higher total polyphenol and flavonoid contents than other samples. However, there was no significant difference in antioxidant activity between samples. These results indicate that strain 21 is the most suitable starter as acetic acid bacteria for producing farm-made vinegar.

This study was carried out to verify the effect of increasing the ambient temperature around apple trees by directly blowing warm-air under the slender spindle apple training system using an agricultural warm-air blower and ducts used in plastic house cultivation to develop late frost damage reduction technology during the blooming season. The temperature increase effect around apple trees owing to the operating warm-air blowing ducts was most evident at a height of 30 cm from the ground surface. At this height, the branch ducts made of Oxford fabric and Solartex showed a raised ambient temperature of about 2.6oC and 1.1oC, respectively. However, the temperature rising effect at a height of 130 cm and 230 cm from the ground surface owing to the operating warm-air blowing ducts was not distinct compared to that in the control. The effect of raising ambient temperature around apple trees through the operation of warm-air blowing ducts was found to be greater as the ambient temperature did not severely drop below the freezing temperature. Damage to the central flower in the apple inflorescence at the pink stage was significantly reduced in the warm-air blowing ducts made of Oxford fabric (42.8%) compared to that in the control (73.2%). However, the damage to other flowers except the central one was not significant compared to that in the control. The fruiting rate of the central and other flowers in the apple inflorescence depending on the operating warm-air blowing ducts was significantly increased compared to that in the control. The fruiting rates of central and other flowers depending on the ducts materials, such as Oxford fabric, and Solartex, and that of the control were 38.3%/82.7%, 31.4%/82.7%, and 0.5%/61.1%, respectively. In conclusion, in the case of open-field apple orchards, if the warm-air blowing duct is installed close to the laterals where frost damage mainly occurs in the slender- spindle dense cultivation system, the damage caused by late frost in the vicinity of blooming time can be reduced to some extent.

Dried apple jeonggwa, a traditional Korean dessert, was made using various apple varieties, such as Jonathan, Fuji, and Hongro. After making the dried apple jeonggwa, the physical and sensory properties of the dessert made using different apple varieties were compared. The apple varieties had no differences in moisture content, weight loss, or sugar content. Chromacity differences were observed depending on the varieties, but E was small. Hardness was highest in Jonathan, while resilience was highest in Fuji, but there was no significant difference in hardness. In the sensory evaluation, appearance, color, sweetness, hardness, chewiness, and overall acceptability were highest in Jonathan, while there was no substantial difference among the varieties in terms of flavor, sourness, or adhesiveness.

Golden apple snails(GAS) are native to warm regions such as Central and South America and Southeast Asia, and were first introduced as a high-protein food. GAS are omnivorous and have a habit of eating plants submerged in water, so they have been used for eco-friendly weed control in rice fields since 1992. When the GAS was first introduced, it was thought that it would be impossible to overwinter in Korea. However after 2000, overwintering individuals were founded and damage to rice occurred and the development of means to control GAS has been required. In this study, we tested the effectiveness of an eco-friendly pest control agent using Styrax japonicus that grow naturally in Korea. As a result of exposing GAS to S. japonicus fruit powder, a 100% molluscicidal effect was confirmed at 66.7ppm. To investigate the duration of effect, treatment was performed at the same concentration and molluscicidal effect of more than 90% was up to 3 days after treatment. The killing effect of each part of the S. japonicus was compared, and the seed extracts showed no killing effect at all concentrations, while the sarcocarp extracts showed a 100% killing effect up to 33.3ppm, and the fruit extracts showed a 100% killing effect up to 200ppm.

Paddy fields provide important habitats for biodiversity conservation within the agricultural ecosystem. Their importance is gradually increasing as their ecological value is better understood. Benthic macroinvertebrates dominate paddy fields. They play an essential role in maintaining the biodiversity of paddy ecosystems. This study aimed to analyze characteristics of benthic macroinvertebrate communities and main environmental factors affecting the distribution of golden apple snails (Pomacea canaliculata). Results showed that the diversity index (H ′) of the benthic macroinvertebrate community was the highest at the Sangju site (St. 12) but the lowest at the Sancheong site (St. 18). Total Dissolved Solids (TDS), salinity, and Electrical Conductivity (EC) values were the highest in Gimhae and Yeongam based on Canonical Correspondence Analysis (CCA). Numbers of P. canaliculata (m-2) were relatively low in Gunsan and Iksan where water temperatures were high. Therefore, changes in geographical characteristics and environmental factors might affect the distribution of P. canaliculata and characteristics of benthic macroinvertebrate communities. Results of this study can be used as primary data for biodiversity conservation and ecosystem service evaluation in agroecosystems.

Biomass carbon materials with high rate capacity have great potential to boost supercapacitors with cost effective, fast charging– discharging performance and high safety requirements, yet currently suffers from a lack of targeted preparation methods. Here we propose a facile FeCl3 assisted hydrothermal carbonization strategy to prepare ultra-high rate biomass carbon from apple residues (ARs). In the preparation process, ARs were first hydrothermally carbonized into a porous precursor which embedded by Fe species, and then synchronously graphitized and activated to form biocarbon with a large special surface area (2159.3 m2 g− 1) and high degree of graphitization. The material exhibited a considerable specific capacitance of 297.5 F g− 1 at 0.5 A g− 1 and outstanding capacitance retention of 85.7% at 10 A g− 1 in 6 M KOH, and moreover, achieved an energy density of 16.2 Wh kg− 1 with the power density of 350.3 W kg− 1. After 8000 cycles, an initial capacitance of 95.2% was maintained. Our findings provide a new idea for boosting the rate capacity of carbon-based electrode materials.

This study aimed to investigate the physicochemical characteristics and antioxidant activity of cereal bars containing various concentrations (0, 10, 20, or 30%) of apple pomace (AP). Highest vitamin B1 and C contents were observed in 30% AP-containing bars, but vitamin B2 and B3 contents were highest in the control (0.347 and 3.566 mg/100 g, respectively). Cutting strength decreased significantly in an AP concentration-dependent manner. Total polyphenol and flavonoid contents in 10% AP bars were 2.949±0.157 mg of GAE/g and 1.001±0.009 mg of CE/g, respectively. Antioxidant activities were assessed by measuring free radical scavenging activities, and 10% AP bars had the highest activities, as determined by ABTS (2,2'azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (α-α-diphenyl-β-picrylhydrazyl) assays (0.412±0.008 and 0.492±0.021 mg GAE/g, respectively). These results suggested that apple pomace should be regarded a potential nutritional and antioxidant source.

To confirm the applicability of crab apple, the composition and content of amino acids and minerals with ‘Fuji’ apple were measured. Apple and crab apple were extracted with water, 70% and 100% ethanol, respectively. The contents of polyphenols, flavonoids, and ascorbic acid, antioxidant activities of these extracts were measured. The amino acid composition of apples comprised a total of 17 amino acids. The total amino acid contents of apple and crab apple were 2,050.45 mg/kg and 900.05 mg/kg, respectively. For minerals, the total mineral content of apple and crab apple were 489.14 mg%, 529.77 mg%, respectively. The contents of polyphenols, flavonoids, and ascorbic acid of apple and crab apple extracts were highest in 70% ethanol extracts. The content of polyphenols, the crab apple extract, showed a generally higher content than the apple extract. The content of flavonoids, apple and crab apple extracts revealed no difference between extracts. The content of ascorbic acid, apple extract showed a generally higher content than the crab apple extract, but there was no significant difference between extracts. In the case of DPPH and ABTS radical scavenging abilities, 70% ethanol extract showed the highest antioxidant activity, and crab apple showed higher activity than apple extracts.

본 연구는 RGB, 초분광 센서를 이용하여 시기별 사과 잎의 엽록소와 질소 함량을 예측하여 사과 나무 잎의 질소 영양을 진단하기 위해 수행되었다. 분광 데이터는 사과나무 ‘홍로 /M.9’ 2년생을 대상으로 고해상도 RGB와 초분광 센서로 촬 영 후 영상처리를 통해 취득하였다. 식물체 데이터는 촬영이 끝난 직후 엽록소와 잎 질소 함량을 측정하였다. 엽록소 측정 기의 SPAD meter, RGB 센서의 개별 파장, 컬러 식생지수 및 초분광 센서의 214개의 파장과 식물체 데이터를 이용하여 회 귀분석을 실시하였다. 엽록소와 잎 질소 함량 데이터는 시기 와 상관없이 질소 시비량에 따라 통계적으로 유의한 차이가 나타났다. 잎은 시기가 지나면서 잎에 있던 영양분이 과실로 전이되어 색이 옅어졌으며 RGB센서의 경우 Red파장에서 시 기와 상관없이 통계적으로 유의한 차이가 나타났다. 초분광 센서의 경우 두 시기 모두 질소 시비 수준에 따라 가시광 영역 보다 비가시광 영역에서 차이가 크게 나타났다. 반사값를 이 용하여 식물체 특성의 예측 모델 결과 엽록소, 잎 질소함량 모 두 초분광 데이터를 이용한 부분최소제곱 회귀분석을 이용하 였을 때 성능이 가장 높게 나타났다(chlorophyll: 81% / 63%, leaf nitrogen content: 81% / 67%). 이러한 원인은 RGB 센서 에 비해 초분광 센서는 좁은 FWHM과 400－1,000nm의 넓 은 파장 범위를 가지고 있어 질소 결핍에 의한 스트레스로 인 해 작물의 분광학적 해석이 가능했을 것으로 판단된다. 추후 분광학적 특성을 이용하여 전 생육 시기의 수체 생리, 생태 모 델 개발 및 검증 그리고 병해충 진단 등 연구를 통해 고품질, 안 정적인 과실 생산 기술 개발에 기여될 것으로 사료된다.