Bovine rotavirus (BRV) is one of the common gastrointestinal diseases that can occur in calves, causing severe diarrhea. This study was conducted to investigate the effects of oral administration of single-domain antibodies, single variable domain of heavy chain of HCAb (VHH), on the prevention and treatment of rotavirus-induced diarrhea in calves. Thirty calves were divided into five experimental groups (negative control, positive control, group A, group B and group C). Except for the negative control group, the experimental groups were inoculated with BRV on the 4th day after birth. The VHH supplementation groups were fed with a substitute milk containing different concentrations of VHH (1%, 2.5% and 7.5%). Changes in body weight, clinical symptoms, serum antibodies, and virus detection in feces were observed for a total of 10 days in each experimental group. The results showed a higher survival rate in the VHH supplementation groups compared to the positive control group. Clinical symptoms caused by rotavirus were alleviated in the VHH supplementation groups. The detection rate of rotavirus in feces also decreased. Gross findings indicated that the severity of disease in the VHH supplementation groups was milder than that in the positive control group. Therefore, VHH could be proposed as a useful alternative for the prevention and treatment of rotavirus-induced diarrhea in calves.

Obesity, characterized by excessive fat accumulation, poses global health risks, including metabolic disorders like type 2 diabetes and cardiovascular diseases, with its prevalence steadily rising. This study proposes that rottlerin induces anti-obesity effects by enhancing non-shivering thermogenesis in beige adipocytes D16 via LRP6 inhibition. As a result, treatment of D16 cells with rottlerin up to 5 mM showed no cytotoxicity. Rottlerin significantly increased the expression of proteins involved in substrate oxidation, such as UCP1 and PGC1a, while decreasing the expression of C/EBPb associated with adipogenesis. Additionally, PRDM16, regulating brown adipocyte development, exhibited increased expression. The phosphorylation of LRP6, an indicator for Wnt signaling and nutrient-sensing pathway, is decreased by rottlerin. In conclusion, the study highlights the reduced phosphorylation of LRP6 as a pivotal mechanism by which rottlerin promotes the “beigeing” of D16 adipocytes, subsequently inducing non-shivering thermogenesis. This underscores rottlerin’s potential as a natural bioactive compound with anti-obesity effects.

In this study, the expression of genes related to fruit ripening was investigated using realtime PCR. The study aimed to determine the effective conditions of calcium compounds (Calcium citrate, calcium chloride, calcium nitrate, GH-Ca, OS-Ca) and chitosan treatment to extend the shelf-life in the ‘Kumhong’ nectarine and ‘Madoka’ peach fruits. In this study, in the ‘Kumhong’ and ‘Madoka’ fruits, the expression of genes related to cell wall degradation, pectin lyase (PL), polygalacturonase (PG), and pectin methylesterase (PME), was inhibited by calcium, chitosan, or a mixture (Chitosan+CaCl2) treatment. In ‘Madoka’ peach, although the expression of the PG gene was suppressed at pre-harvest Ca and chitosan treatment, the expression of the PL gene was induced at pre- and post-harvest Ca and chitosan treatment compared to the control. The spray of calcium, chitosan itself, or a mixture (Chitosan+ CaCl2) on the trees at the ripening stage and harvested fruits can extend the shelf-life by suppressing the expression of cell wall degrading enzymes genes (PL, PG, PME) in ‘Kumhong’ nectarine and ‘Madoka’ peach tree. These results provide valuable information for the development of technology for the extension of the shelf-life of peach and nectarine fruits.

배경/목적: ROS는 악성종양의 성장 및 공격과 관련이 있다. UDCA는 담도암 세포에서 진행과 전이에 밀접한 EGFR-MAPK 신 호 경 로 와 EMT를 억 제 한 다 . 이 연 구 는 UDCA가 담도암세포에서 ROS 생성 및 그에 관련된 바이오마커에 어떠한 영향을 주는지 알아보기 위해 시행되었다. 방 법 : 인간 간외 담관암 세포주인 SNU-245세포를 배양하였다. 세포생존율은 MTT assays로, ROS는 세포 ROS assays kit로 측정하였다. Western blotting으로 다양한 표적 단백질의 발현 수준을 측정하였다. 특정 유전자의 억제를 위해 siRNA를 사용하였고, 특정 유전자의 과발현을 위해 shRNA를 사용하였다. 결과: UDCA는 담도암 세포에서 DCA에 의한 peroxide와 ROS가 생성되는 것을 억제하였으며, DCA로 발현이 증강된 STAT3, PRX2 및 SOD2를 억제하였고, IGF-1에 의해 발현이 증강된 NOX2 및 NOX4를 억제하였다. 또한, 담도암 세포에서 SiRNA를 이용한 STAT3 및 PRX2의 억제는 UDCA 처치와 상관없이 EGF에 의해 약화된 E-cadherin 발현을 복원하고 EGF에 의해 증가된 N-cadherin 발현을 억제하였는데, 이는 UDCA의 EMT 억제에 PRX2/STAT3가 상당한 역할을 하는 것을 의미한다. 덧붙여, UDCA는 담도암 세포에서 DCA에 의해 억제된 catalase의 발현을 복원하였다. 한편, ShRNA를 사용한 NOX4의 과발현의 유도는 UDCA의 항종양 효과를 상쇄하였다. 결론: UDCA는 담도암 세포에서 ROS 생성을 억제하고, ROS 제거를 향상시킴으로써, 결국 EMT와 관련된 STAT3 및 PRX2를 억제한다, 따라서, UDCA는 ROS 활성도 및 EMT의 억제를 통하여 담도암 세포의 성장 및 침습을 억제하는 데 기여한다.

To develop a functional probiotic that inhibits gingipain, a major virulence factor of Porphyromonas gingivlais (P. gingivalis), we screened over 30 probiotic strains for their ability to inhibit gingipian activity. We investigated the inhibition of expression of gingipain genes kgp, rgpA, and rgpB as well as gingipain activity, using freeze dried cell-free supernatants of Weisiella cibaria SPM402 (WC402) and Lactobacillus paracasei SMP412 (LP412), both of which demonstrated antibacterial activity against P. gingivalis. Thus, it was verified that kgp expression was reduced by approximately 0.71±0.02 folds and rgpB expression was reduced by approximately 0.71±0.14 folds at a concentration of WC402 10 mg/mL. Meanwhile, at the same concentration of 10 mg/mL of LP412, kgp expression was reduced by approximately 0.19±0.08 folds, rgpA expression was reduced by approximately 0.09±0.02 folds, and rgpB expression was reduced by approximately 0.24±0.03 folds. At a concentration of 10 mg/mL, Kgp activity was inhibited by approximately 78.65±3.58% (cell associated gingipain, CAG), 82.45±1.22% (cell-free gingipain, CFG) by WC402 and 80.71±2.11% (CAG), and 85.81±0.05% (CFG) by LP412 respectively. Rgp activity was also effectively inhibited by approximately 78.6±1.01% (CAG), 86.78±0.47% (CFG) and 82.93±1.26% (CAG), 88.81±0.36% (CFG) by WC402 and LP412 respectively. Based on these results, W. cibaria SPM402 and L. paracasei SPM412 can be regarded as functional probiotics with the ability to inhibit gingipain activity and exhibit antibacterial effects against P. gingivalis.

Cholesterol is prone to oxidation, which results in the formation of cholesterol oxidation products (COPs). This occurs because it is a monounsaturated lipid with a double bond on C-5 position. Cholesterol in foods is mostly non-enzymatically oxidized by reactive oxygen species (ROS)-mediated auto-oxidative reaction. The COPs are found in many common foods of animal-origin and are formed during their manufacture process. The formation of COPs is mainly related to the temperature and the heating time the food is processed, storage condition, light exposure and level of activator present such as free radical. The level of COPs in processed foods could reach up to 1-10 % of the total cholesterol depending on the foods. The most predominant COPs in foods including meat, eggs, dairy products as well as other foods of animal origin were 7-ketocholesterol, 7 α-hydroxycholesterol (7α-OH), 7β-hydroxycholesterol (7β-OH), 5,6α-epoxycholesterol (5,6α -EP), 5,6β-epoxycholesterol (5,6β-EP), 25-hydoxycholesterol (25-OH), 20-hydroxycholesterol (20-OH) and cholestanetriol (triol). They are mainly formed non-enzymatically by cholesterol autoxidation. The COPs are known to be potentially more hazardous to human health than pure cholesterol. The procedure to block cholesterol oxidation in foods should be similar to that of lipid oxidation inhibition since both cholesterol and lipid oxidation go through the same free radical mechanism. The formation of COPs in foods can be stopped by decreasing heating time and temperature, controlling storage condition as well as adding antioxidants into food products. This review aims to present, discuss and respond to articles and studies published on the topics of the formation and inhibition of COPs in foods and key factors that might affect cholesterol oxidation. This review may be used as a basic guide to control the formation of COPs in the food industry.

본 연구는 퍼레니얼 라이그래스에서 생장조정제 trienexapacethyl과 prohexadione-calcium을 처리하였을 때 생육 시기별 잔디 품질과 생육 억제 효과를 조사하였다. 처리구는 대조구 (Control), TE 처리구(0.01 a.i. g·m-2·100mL-1) 및 PC 처리구 (0.01 a.i. g·m-2·100mL-1)으로 설정하였다. 생장조정제 처리 후 퍼레니얼 라이그래스의 엽색 지수 및 엽록소 함량은 대조 구와 통계적으로 유의적인 차이를 나타내지 않았다. TE와 PC 처리구에서 잔디의 초장은 각각 8.9－12.4%와 6.7－13.6% 씩 감소하였고, 잔디 예지물량은 44.4－45.8%와 40.6－ 40.9%씩 감소하였다. 생장조정제 처리 후 경과 일수와 초장 의 변화를 이용하여 약효 지속 기간을 조사하였을 때, TE와PC 처리구는 1차 조사(7월 29일－8월 26일)에서 각각 43.6일과 37.9일, 2차 조사(9월 30일－10월 28일)에서는 38.3일과 39.5일이었다. 상기 결과들을 종합할 때, TE와 PC의 처리는 퍼레니얼 라이그래스의 초장 및 생육을 억제하였고, 약효 지 속 기간은 약 40 ±3일 정도를 나타냈다. 또한 생장조정제 처리 시 계절별 잔디 생육과 품질에 영향을 미치지 않았으며, 약해 는 발생하지 않았다.

This study was carried out to determine the sprouting period of early and mid-season varieties, which includes ‘Atlantic’, ‘Chubaek’, and ‘Superior’, during the summer storage period in a semi-underground warehouse without cooling system. And also it was investigated the effect of chlorpropham [Propan-2-yl N-(3-chlorophenyl)carbamate, CIPC] treatment on the sprouting inhibition for the varieties. This study was conducted to figure out a sprout inhibitory effect when CIPC was applied to 1kg of the potato tubers at concentrations of 10 mg and 20 mg which are lower than the treatment concentrations of ca 30 mg prescribed by the positive list system (PLS). The internal temperature of the warehouse used in this experiment was lowered by 5°C or more than the outside temperature. The difference between the lowest and highest temperature during the experiment throughout the day was 5°C. It showed the effect of reducing to 1/2 of the difference in outdoor temperature. As for the sprouting of potatoes, the extremely early variety ‘Chubaek’ sprouts appeared at the 6th week of storage of control and it was the fastest sprouting potato among the control groups of the varieties. Sprouting began to appear in the Superior at the 6th week of storage, while the ‘Atlantic’ sprouted at the 8th week of storage. The appearance of sprouts was suppressed in all treatment groups of ‘Atlantic’ and ‘Superior’ varieties in CIPC treatments. Sprouts were observed in all treatment groups of ‘Chubaek’ after the 7th week, but the elongations of the sprouts in tubers were completely inhibited until the 8th week of storage. ‘Atlantic’ and ‘Superior’ seemed to have a sprouting inhibitory effect even with a low CIPC concentration of 10 mg·kg-1, with the exception of extremely early variety ‘Chubaek’ that breaks out of the dormancy quickly. Although weight loss occurred continuously during storage, it was minor loss of 0.7-1.6%. There was no consistent trend for changes of the loss in the varieties and CIPC treatments. Most common pathological disorder was the dry rot during the experiment, but only few were affected. The use of the tubers treated at 18°C and 90% RH for 10 days and the rack of refrigeration system which lead to lack of convection seemed to have suppressed the spread of pathogens.

The objective of this study is to evaluate the antioxidant components, elastase inhibition activities, and melanin synthesis rates of Korean traditional Actinidia (Actinidia arguta) fruits and leaves depending on the ethanol extraction concentrations. The total polyphenol content was the highest in the 50% ethanol extract of both fruits and leaves, with values of 634.1 mg GAE/100 g and 3,985.2 mg GAE/100 g, respectively. The total flavonoid content was the highest in the fruit 90% extract and leaf 50% extract at 191.9 mg/100 g and 2655.6 mg/100 g, respectively. The vitamin C content was the highest in the 50% extract of leaves at 2990.3 mg/100 g. Elastase inhibition was the highest at 56.9% in the leaf 50% extract at a concentration of 1,000 μg/mL. Melanin synthesis inhibition showed the highest melanin synthesis inhibitory effect among the extracts, as the leaf 50% extract showed an inhibitory rate of 65% or more. Therefore, the antioxidant components, elastase inhibition activities, and melanin synthesis inhibitory rate were better in leaves than in fruits. The leaf 50% extract was particularly the best among the extracts. Korean traditional Actinidia leaves can be considered as potential sources for new functional materials.

The germination process is critical for plant growth and development and it is largely affected by environmental stress, especially salinity. Recently, hydrogen sulfide (H2S) is well known to act as a signaling molecule in a defense mechanism against stress conditions but poorly understood regulating seed germination. In this study, the effects of NaHS (the H2S donor) pretreatment on various biochemical (hydrogen peroxide (H2O2) content and amylase and protease activity) and physiological properties (germination rate) during seed germination of oilseed rape (Brassica napus L. cv. Mosa) were examined under salt stress. The seed germination and seedling growth of oilseed rape were inhibited by NaCl treatment but it was alleviated by NaHS pretreatment. The NaCl treatment increased H2O2 content leading to oxidative stress, but NaHS pre-treatments maintained much lower levels of H2O2 in germinating seeds under salt stress. Amylase activity, a starch degradation enzyme, significantly increased over 2-fold in control, NaHS pretreatment, and NaHS pretreatment under NaCl during seed germination compared to NaCl treatment. Protease activity was highly induced in NaHS-pretreated seeds compared to NaCl treatment, accompanied by a decrease in protein content. These results indicate that NaHS pretreatment could improve seed germination under salt stress conditions by decreasing H2O2 accumulation and activating the degradation of protein and starch to support seedling growth.

비가열 닭고기 분쇄육의 제조 시 향신료의 첨가에 의한 세균의 증식 억제 효과에 대하여 연구하였다. 닭가슴살 분 쇄육의 일반성분은 수분 72.98±0.15%, 조단백질 23.37±0.46%, 조지방 1.00±0.03%, 조회분 1.90±0.03%였다. 향신료 첨가 에 의한 미생물 증식 억제 효과는 로즈마리 > 마늘 > 겨 자 순이었으며, 첨가량이 증가할수록 억제 효과가 커졌다. 닭고기 분쇄육의 일반세균 및 대장균 증식 억제를 나타내 는 향신료의 최적 첨가 농도는 로즈마리 2%, 마늘 4%, 겨 자 1.2%였다. 일반세균 및 대장균에 대한 증식 억제 효과 는 단독 및 혼합 첨가 시 저장기간 동안 기간별로 차이가 있었고, 저장 9일째 억제 효과는 MixA(97.4%) > 로즈마 리(96.9%) > MixB(96.3%) > 마늘(53.7%) > 겨자(33.3%) 순이었다. 닭고기 분쇄육에 살균마늘과 비살균마늘을 첨가 하여 비교했을 때 일반세균 수는 살균마늘 처리구가 비살 균마늘 처리구에 비해 초기 2.6-3.0 log CFU/g, 9일째 2.4- 3.2 log CFU/g 정도 낮았고, 저장기간이 경과할수록 그 수 가 감소하였다. 비살균마늘 처리구는 대조구보다 높은 일 반세균 수를 나타냈으며, 저장기간이 경과할수록 증가하는 경향을 나타냈다. 대장균 수의 경우 살균마늘 처리구가 비 살균마늘 처리구에 비해 저장 0일째 0.4-1.0 log CFU/g, 9 일째 0.5-1.5 log CFU/g 정도 낮았으며, 저장기간의 경과에 따른 변화는 일반세균 수와 비슷한 경향을 나타냈다. 결론 적으로 로즈마리, 마늘, 겨자의 다양한 혼합적용에 의해 닭 고기 분쇄육 제품의 미생물적 안전성이 향상되었다.

The bones of the human body support the structures of the body and provide protection for a person’s internal organs. Bone metabolic diseases are on the rise due to a significant increase in life expectancy over a short period of time. Therefore, we investigated the osteoblast differentiation promoting and osteoclastogenesis inhibitory activities of fermented Benincasa hispida cong. (HR1901-BS, HR1901-BSaf). We evaluated the alkaline phosphatase (ALP) activity of MC3T3-E1 mouse calvarial-derived osteoblasts. We also evaluated expression of ALP, osteocalcin (OCN), and runt-related transcription factor 2 (Runx2), which regulate osteoblast differentiation. To assess effects on osteoclast formation, tartrate-resistant acid phosphatase (TRAP) activity in RAW264.7 cells was analyzed. ALP activity increased by 121-136% and 140-156%, respectively in the presence of HR1901-BS and HR1901- BSaf. Expression of osteoblast differentiation factor also increased significantly. We also confirmed that HR1901-BS and HR1901-BSaf decreased TRAP activity in osteoclasts by 35-47% and 23-39%, respectively. Our results showed that fermented Benincasa hispida cong. (HR1901-BS, HR1901-BSaf) increase bone mineralization and osteoblast differentiation activity in MC3T3-E1 cells, and inhibit bone resorption activity in RAW264.7 cells. In conclusion, fermented Benincasa hispida cong. (HR1901-BS, HR1901-BSaf) can be used as an effective natural resource for preventing and treating bone-related diseases.

Lysophosphatidic acid (LPA) is a bioactive lipid messenger involved in the pathogenesis of chronic inflammation and various diseases. Recent studies have shown an association between periodontitis and neuroinflammatory diseases such as Alzheimer’s disease, stroke, and multiple sclerosis. However, the mechanistic relationship between periodontitis and neuroinflammatory diseases remains unclear. The current study found that lysophosphatidic acid receptors 1 (LPAR1) and 6 (LPAR6) exhibited increased expression in primary microglia and astrocytes. The primary astrocytes were then treated using medium conditioned to mimic periodontitis through addition of Porphyromonas gingivalis lipopolysaccharides, and an increased nitric oxide (NO) production was observed. Application of conditioned medium from human periodontal ligament stem cells with or without LPAR1 knockdown showed a decrease in the production of NO and expression of inducible nitric oxide synthase and interleukin 1 beta. These findings may contribute to our understanding of the mechanistic link between periodontitis and neuroinflammatory diseases.

Asthma is a chronic inflammatory disease characterized by recurring symptoms, airflow obstruction, and bronchial hyper-responsiveness. The onset of asthma for most patients begins early in life, and current asthma treatment with anti-inflammatory agents can have adverse effects, eventually leading to impaired quality of life. In the pathogenesis of asthma, macrophages and basophils play a vital role during progression. Macrophages not only induce inflammation by secreting inflammatory cytokines but also promote DNA damage and mucus production through nitric oxide (NO) production. Basophils enhance eosinophil recruitment and aggravate asthma through the FcεRIα receptor with high affinity for histamine and IgE. Therefore, in this study, we investigated whether the activation of macrophages and basophils is suppressed by the individual extracts of 28 natural products. RAW 264.7 cells (mouse macrophages) were treated with the natural products in LPS, and 4 natural product extracts resulted in decreased NO production. In β-hexosaminidase assay using RBL-2H3 cells (rat basophils), 19 natural product extracts decreased β-hexosaminidase production. In NO production and β-hexosaminidase assay using macrophages and basophils, 3 natural product extracts (Plantago asiatica, Centella asiatica, and Perilla frutescens var. japonica) significantly inhibited NO production and β-hexosaminidase release. Overall, we examined the inhibitory effects of 28 natural product extracts on macrophage and basophil activity, and the findings demonstrated the potential of natural product extracts for treating asthma and macrophage- and basophil-related diseases.

The consumption of Flammulina velutipes mushroom imported from Korea has been associated with the cases of listeriosis in the United States, Canada, and Australia. We investigated the effect of sanitizing the plastic wrapper (used in packaging F. velutipes) with slightly acidic electrolyzed water (SAEW) and ultraviolet C waterproof light-emitting diode (UVC-WLED) on reducing the Listeria monocytogenes. Further, the effect of UVC-LED on L. monocytogenes growth in F. velutipes at different storage temperatures (2, 4, and 10oC) was determined. The combined (SAEW+UVC-W-LED) treatment for 5–10 min reduced 99.9% of bacterial population from the contaminated plastic wrapper. In addition, the UVC-LED treatment for 3 min reduced the L. monocytogenes concentration in F. velutipes by 0.47 log CFU/g. Moreover, the growth of L. monocytogenes in the treated mushrooms was slower than that of the untreated (control) ones. L. monocytogenes concentration in F. velutipes increased over 3 log CFU/g at 2oC and 10oC for 60 and 10 days, respectively. The growth of L. monocytogenes at the bottom of mushrooms was faster than that at the top at both the temperatures. These results indicate that the combined SAEW+UVC-WLED treatment of plastic wrappers and the UVC-LED treatment of mushrooms can be used as potential hurdle technologies to control the risk of L. monocytogenes in mushrooms prior to packaging at farms.

Background: Neck pain is a major health problem in developed countries and has a lifetime prevalence of 50%. Major problems include a reduced cervical range of motion, muscle stiffness, dysfunction, postural changes, and decrease in psychosocial level. Objects: This study aimed to investigate the effects of applying the upper trapezius inhibition dynamic taping to patients with chronic neck pain on their neck pain, functional level, cervical range of motion, psychosocial level, and neck posture. Methods: The study design was a randomized controlled trial. A total of 40 patients with neck pain participated in this study and were randomly assigned to a Dynamic Taping group (n = 20) or Sham Taping group (n = 20). In both groups, basic intervention cervical pain control therapy and shoulder stabilization exercise program were performed. In addition, dynamic taping and sham taping were applied to participants in the Dynamic Taping and Sham Taping groups to inhibit the trapezius muscle, respectively. All interventions were performed three times a week and a total of 12 times for 4 weeks, and the participants’ neck pain, functional impairment level, cervical range of motion, psychosocial level, and neck posture were measured and compared before and after the intervention. Results: Both groups showed significant differences in neck pain, functional level, cervical range of motion, psychosocial level, and neck postural before and after the intervention (p < 0.05). Moreover, there were significant differences between the two groups regarding the functional level and neck posture (p < 0.05). Conclusion: Inhibition dynamic taping of the upper trapezius muscle suppression is an effective method with clinical significance in reducing pain in individuals with chronic neck pain and improving the functional level, cervical range of motion, psychosocial level, and neck posture.

Scale and rust generation in water pipes is a common phenomenon when cast iron water pipes have been used for a long time. A physical water treatment device is known among various means for suppressing rust in a water pipe, and a zinc ionization device for putting zinc metal into a pipe and emitting the zinc cation into water is one of such devices. This research measured the amount of zinc ion generated, which is known to exhibit an effect of inhibiting rust and scale generation in a pipe, and examined the scale and rust inhibition effect of the ionization device installed for ground or building water supply. In the case of distilled water, the concentration of zinc ion increased by circulating water in the ionization device several times, and it was verified to be hundreds of μg/L, and in the case of discharging ground or tap water, it was verified to be tens of μg/L. In addition, a verification pipe was installed to confirm the change inside the pipe before and after installation of the zinc ionization device, and the internal condition of the pipe was observed 3 months to several years after installation. It was confirmed that the corrosion area of the surface of the pipe was no longer increased by installing a corrosion inhibitor, and if the pipe was already filled with corrosion products, the amount of corrosion products gradually decreased every year after installation. The phenomenon of fewer corrosion products could be interpreted as expanding the space in the pipe due to the corrosion product as Fe2O3 adhered to the inner surface of the pipe and turned into a smaller black Fe3O4. In addition, we found that scale such as CaCO3 together in the corrosion by-products gradually decreased with the attachment of the ionization device.

Periodontitis and periimplantitis are caused as a result of dental biofilm formation. This biofilm is composed of multiple species of pathogens. Therefore, controlling biofilm formation is critical for disease prevention. To inhibit biofilm formation, sugars can be used to interrupt lectin-involving interactions between bacteria or between bacteria and a host. In this study, we evaluated the effect of D-Arabinose on biofilm formation of putative periodontal pathogens as well as the quorum sensing activity and whole protein profiles of the pathogens. Crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy revealed that D-Arabinose inhibited biofilm formation of Porphyromonas gingivalis , Fusobacterium nucleatum , and Tannerella forsythia . D-Arabinose also significantly inhibited the activity of autoinducer 2 of F. nucleatum and the expression of representative bacterial virulence genes. Furthermore, D-Arabinose treatment altered the expression of some bacterial proteins. These results demonstrate that D-Arabinose can be used as an antibiofilm agent for the prevention of periodontal infections.