This study explored the suitability of nurungji as a substitute for malt. Rice beers (R20, R40, R60) and nurungji beers (N20, N40, N60) were prepared by substituting 20%, 40%, and 60% malt with the rice and nurungji, respectively. All samples were fermented at 25oC for 5 days and stored at 2oC for 15 days to study quality characteristics. The pH value of wort solution was the highest at R60. The viable cell counts of all samples were not significantly different during fermentation and storage. As the amount of rice and nurungji increased, the L value in chromaticity increased, whereas the a and b values tended to decrease during fermentation and storage. The reducing sugar, soluble solid contents, and specific gravity of the wort solution were higher in nurungji beer than in rice beer. After fermentation and storage, the alcohol content of N40 was 5.83%, the highest among the prepared rice and nurungji beers. The total polyphenol, flavonoid contents, DPPH, and ABTS scavenging activity of beer decreased with increased rice and nurungji substitution ratios during fermentation and storage. Notably, nurungji beer showed higher antioxidant activity than rice beer during fermentation and storage.
This study investigated the inactivation effect of intense pulsed light (IPL) on various packaging films. The UV-C transmittance irradiance varied depending on the packaging materials, with the control group having an irradiance of 3.959 W/m2. For the thinnest layer of 30 mm, polypropylene (PP) had an irradiance of 3.258 W/m2, polyethylene (PE) had an irradiance of 3.193 W/ m2, and oriented polypropylene (OPP) had an irradiance of 3.200 W/m2. In contrast, polyethylene terephthalate (PET) exhibited a significantly lower irradiance of only 0.065 W/m2 for its thinnest film thickness of 100 mm. The light transmittance of the packaging materials was similar, with values of 91.3%, 89.7%, and 89.5% for PP, PE, and OPP, respectively. In contrast, PET exhibited a significantly lower light transmittance of 1.8% compared to the other packaging materials. These findings have practical implications for the food packaging industry. According to the packaging film material, the sterilization effects showed that the E. coli sterilization effect of PP was the highest, followed by PE and OPP, which were similarly effective. At the same time, PET exhibited the lowest sterilization effect. For PP film with a thickness of 30 mm, a 6.2 log reduction in the E. coli population was observed after 20 s of IPL treatment. Ultimately, inactivation was achieved after 60 seconds. Both PE and OPP films, which had a thickness of 30 mm, showed a 5.9 log reduction in E. coli after 30 s of IPL treatment, followed by complete inactivation after 60 s. The inactivation rate for PP, PE, and OPP films showed minimal variation regardless of thickness, although it gradually decreased with increasing thickness. For PET, achieving a 1 log reduction in E. coli required 180 s of IPL treatment at 100 mm thickness and 210 s at 120 mm thickness, indicating the influence of film thickness on inactivation rate. Even after 300 s of IPL treatment, the inactivation effect for PET remained around 1.5 log, the lowest among all packaging film materials.
Obesity, characterized by excessive fat accumulation, poses global health risks, including metabolic disorders like type 2 diabetes and cardiovascular diseases, with its prevalence steadily rising. This study proposes that rottlerin induces anti-obesity effects by enhancing non-shivering thermogenesis in beige adipocytes D16 via LRP6 inhibition. As a result, treatment of D16 cells with rottlerin up to 5 mM showed no cytotoxicity. Rottlerin significantly increased the expression of proteins involved in substrate oxidation, such as UCP1 and PGC1a, while decreasing the expression of C/EBPb associated with adipogenesis. Additionally, PRDM16, regulating brown adipocyte development, exhibited increased expression. The phosphorylation of LRP6, an indicator for Wnt signaling and nutrient-sensing pathway, is decreased by rottlerin. In conclusion, the study highlights the reduced phosphorylation of LRP6 as a pivotal mechanism by which rottlerin promotes the “beigeing” of D16 adipocytes, subsequently inducing non-shivering thermogenesis. This underscores rottlerin’s potential as a natural bioactive compound with anti-obesity effects.
Buckwheat leaves have the best antioxidant properties, including flavonoids, rutin, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability compared to common buckwheat and tartary buckwheat. The total dietary fiber content of the powder extracted by mixing buckwheat leaves and sea tangle was 30.5%. To manufacture bread containing mixed buckwheat flour, 10, 20, and 30% buckwheat flour was mixed in to produce buckwheat bread. The bread’s characteristics underwent significant changes with varying levels of buckwheat flour. As the buckwheat content increased, the size of the bread decreased, but its hardness, gumminess, and chewiness tended to increase. The sensory characteristics of the bread were not improved due to the addition of gluten in the case of bread mixed with 20% buckwheat flour. When the buckwheat flour content was mixed at 20%, and the mixed extract of buckwheat leaves and sea tangle were added at 0.4% and 1.0%, there was no significant difference in the appearance of the buckwheat bread. Still, regarding sensory properties, the preference was higher in the sample with 1.0% added buckwheat flour.
This study performed the pre-treatments of the manufacturing of the pork skin snack with wet-salting (3% and 6%; 3W and 6W) and dry-salting (0.3% and 0.6%; 0.3D and 0.6D). Regarding the manufacturing yield, the 0.3D and 6W were significantly higher than the other samples (p<0.05). The water contents of the Con and 0.6D were significantly lower than the other samples (p<0.05). The fat contents of the Con and 6W were evidently higher than the 3W, 0.3D, and 0.6D (p<0.05). The lightness of the wet-salting group was markedly higher than the Con (p<0.05). The redness and yellowness of the 0.6D were noticeably higher than the Con (p<0.05). The crispness of the 0.6D was considerably higher than the other samples (p<0.05). The appearance and sensory crispness of the 3W and 0.6D were notably lower than the other samples (p<0.05). The taste score of the 0.6D was much lower than the 6W and 0.3D (p<0.05). The overall acceptability of the Con, 6W, and 0.3D was appreciably higher than the other samples (p<0.05). Overall, this study showed that wet-salting with 6% (6W) and dry-salting with 0.3% (0.3D) can improve pork skin snack quality properties.
This research presented the procedural framework of developing and optimizing an artificial intelligence model for predicting the change of bread texture by different baking enhancers. Emphasis was placed on the impact of various baking enhancers on the Mixolab thermo-mechanical properties of wheat flour and consequent alterations in bread texture. The application of baking enhancers positively contributed to dough formation and stability, producing bread with a soft texture. However, a relatively low Pearson correlation coefficient was observed between a single Mixolab parameter and bread texture (r<0.59). To more accurately predict the texture of bread from the thermo-mechanical features of wheat flour with baking enhancers, five AI models (multiple linear regression, decision tree, stochastic gradient descent, random forest, and multilayer perceptron neural network) were applied, and their prediction performance was compared. The multilayer perceptron neural network model was further utilized to enhance the prediction of bread texture by mitigating overfitting risks. Finally, the hyperparameter tuning (activation function [Leaky ReLU], regularization [0.0001], and dropout [0.1]) led to enhanced model performance (R2 = 0.8109 and RMSE = 0.1096).
Broccoli and cabbage are known to have antioxidant, cholesterol-lowering, and anticancer effects due to their high bioactive component levels. This study applied 70% ethanol and hydrothermal extraction to separate bioactive components from broccoli and cabbage. 30oBrix extracts were freeze-dried and then diluted in various concentrations with distilled water for analysis. Total polyphenol contents, DPPH radical scavenging activity, ABTS radical scavenging activity, ferric ion reducing antioxidant power, and hydrogen peroxide scavenging activity were analyzed. The diverse antioxidant assays, except DPPH radical scavenging activity, showed that hydrothermal extracts had significantly higher activity than the 70% ethanol extracts (p<0.05). In this study, it suggested that hydrothermal extraction is relatively effective in producing broccoli and cabbage extracts. Moreover, hydrothermal extraction conditions could be low-cost and environmentally friendly.