Sanitary management practices from 8 packaged meals (Dosirak) manufacturing establishments were surveyd during production and distribution and their microbiological quality of dosirak items marketed in CVS were analyzed. Correlation coefficients were calculated to deternine significant relationships between sanitary management practices and microbiological quality of packaged meals. The results of self evaluation on sanitary management practices indicated 'doing practice well' in personnel and equipment sanitation management but time-temperature control management were practiced satisfactorily only about 55% as compared with the managerial standard guidelines. Significant relationships between the status of actual sanitary management practices and microbiological quality of Dosirak items were not found, but general status of microbiological quality of Dosirak items revealed positive relationship with the size of operational structure and the status of time-temperature control management practices.

This study was carried out to develop the computer-assisted Hazard Analysis and Critical Control Point (HACCP) program for a systematic approach to the identification, assessment and control of hazards for foodservice manager to assure the microbiological quality of food in hospital foodservice operations. Sanitation practices were surveyed and analyzed in the dietetic department of 4 hospitals. Among them, one 762-bed general hospital was selected as standard model to develop computer-assisted HACCP program. All data base files and processing programs were created by using Foxpro package for easy access of HACCP concept. HACCP program was developed based on the methods suggested by NACMCF, IAMFES and Bryan. This program consisted of two parts: the pre-stage for HACCP study and the implementation stage of the HACCP system. 1. Pre-stage for HACCP study includes the selection of menu item, the development of the HACCP recipe, the construction of a product flow diagram, and printing the HACCP recipe and a product flow diagram. A menu item for HACCP study can be selected from the menu item lists classified by cooking methods. HACCP recipe includes ingredients, their amount and cooking procedure. A flow diagram is constructed based on the HACCP recipe. The HACCP recipe and a product flow diagram are printed out. 2. Implementation of HACCP study includes the identification of microbiological hazards, the determination of critical control points, the establishment of control methods of each hazard, and the complementation of data base file. Potentially hazardous ingredients are determined and microbiological hazards are identified in each phase of the product flow. Critical control points (CCPs) are identified by applying CCP decision trees for ingredients and each process stage. After hazards and CCPs are identified, criteria, monitoring system, corrective action plan, record-keeping system and verification methods are established. When the HACCP study is complemented, HACCP study result forms are printed out. HACCP data base file can be either added, corrected or deleted.

This study researched microbial change of quality according to the various phases of product flow of cooked pea and rice, cold cucumber and seaweed soup, soybean sprouts japchae feeding urban type of a commissary school and a satellite school in Daejeon area, also it suggested the possibility that the central commissary foodservice system can be established and utilized more developmental to identify its food of variation of temperature and state of safety unitl 3 hours after cooking for the case of delay of distribution and holding because of the satellite school of geographical location and traffic problem. The critical Control Points identified for each category of menu items were: Boiled pea and rice: inadequate distribution, holding and storing before assembly; Cold cucumber and seaweed soup: pre-preparation and post-preparation after cooking; Soybean sprouts japchae: Pre-preparation, post-preparation and storing. As the result of observation of the variation of temperature and microbial safety according to the delay of distribution and holding for each food, all of them were relatively safe until 3 hours after cooking, but cold cucumber and seaweed soup being stored for 3 hours, the value of E. coli is 103 CFU/g. The variation of temperature was more extreme in soybean sprouts japchae than cooked pea and rice and cold cucumber and seaweed soup. It was proved that the stainless container was excellent and that adequate holding container should be used.

The main goal of this work was to investigate changes in microbiological quality and dominant bacterial communities present in minimally processed Platycodi radix (Doraji) under vacuum packaging during cold storage using matirix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry fingerprinting. Microbiological analysis of raw Platycodi radix throughout the manufacturing process highlighted the presence of high numbers of total viable cells both before and after storage. Further, the dominant bacterial communities changed during processing and storage, with greater richness detected in Enterobacteriaceae; specifically, Serratia fonticola and Rahnella aquatilis became abundant in Platycodi radix after processing. During storage at 5 and 15℃, the quality diminished and the relative abundance of Leuconostoc spp. increased; Leuconostoc pseudomesenteroides and Leuconostoc citreum were the main bacterial communities in decayed Platycodi radix at the end of storage. Microorganisms present in Platycodi radix products during processing and storage are potentially involved in deterioration. The identification of dominant bacteria can be helpful in the effective establishment of packaging and storage conditions to prolong the freshness of minimally processed Platycodi radix products.

This study was conducted to investigate the effect of chlorine dioxide fumigation as a substitute for sulfur fumigation which has been used as a method to prevent the quality change of persimmon during storage and distribution process. Dried persimmons were treated with chlorine dioxide gas concentration (0, 15 30, and 45 ppm) and time (0, 15, 30, and 45 min) and microbiological changes, texture properties and color of the treated samples were investigated during storage at room temperature. Total aerobic bacteria, yeast and mold numbers after chlorine dioxide gas fumigation were decreased when compared with the control group. The inhibitory effects of total aerobic bacteria, yeast, and mold were observed during storage. The texture properties and color value of dried persimmons were not affected by chlorine dioxide gas fumigation concentration and time. There was no difference in quality between chlorine dioxide gas fumigation treatment group and control group. These results suggested that chlorine dioxide gas fumigation treatment can be utilized as a processing technique to secure microbiological storage stability of dried persimmons.

고추장굴비의 지방산패를 최소화하기 위하여 신선굴비를 동결건조한 굴비포를 사용하여 고추장굴비를 제조하고 비열처리 살균방법으로 초고압과 감마선 처리하여 저장 중에 품질과 미생물변화를 분석함으로서 고추장굴비의 저장과 유통기간 연장 가능성을 검토하였다. 초고압처리(200, 400, 600 MPa) 및 감마선 조사(7, 10, 20, 30 kGy) 후 pH 변화는 유의적으로 차이가 없었으며 20일 동안 상온에서 저장 중에도 시료간의 유의적인 변화를 보이지

본 연구의 목적은 대구 경북지역 초등학교에 공급되는 전처리 채소와 가공식품의 유통단계를 분석하고, 유통과정에서의 미생물 변화와 학교급식에 이용되는 식재료의 미생물 품질을 평가하기 위하여 실시되었다. 유통 중 박피도라지에서 일반세균 CFU/g, 대장균군 CFU/g, E. coli < , S. aureus < CFU/g, B. cereus < CFU/g이 검출되었다. 전처리 양파에서는 일반세균 CFU/g, 대장균군 CFU/g이 검출되

마즙의 위생성 및 보존성 향상을 목적으로 0, 1, 3 및 5 kGy의 선량으로 감마선을 조사하고 에서 보관하면서 8일간 마즙의 미생물학적, 일반품질 및 관능평가를 실시하였다. 마즙의 호기성세균 및 대장균군은 각각 1.26 및 1.34 kGy의 값을 보였으며, 저장에 따라 3 및 5 kGy 조사구의 경우 검출한계 이하의 균수를 유지하였다. 효모 및 곰팡이의 D10값은 0.29 kGy로 일반호기성균 및 대장균군 보다 감마선에 높은 감수성을 보였으

명란젓갈은 한국 전통 발효 식품중 하나로, 본 연구에서는 명란젓갈의 유통기한 연장방안으로 감마선 조사를 적용하여 미생물학적, 관능적 품질 실험을 행하였다. 명란젓갈과 고춧가루, 생강, 마늘, 복합시즈닝 믹스 등의 부재료에 각각 0, 0.5, 1.0, 2.0 및 5.0 kGy의 선량으로 감마선 조사를 하여 에서 4주간 저장하며 관찰하였고, 이들의 수분활성도는 각각 0.89, 0.56, 0.98, 0.99 및 0.07 이었다. 명란젓갈의 초기 호기성

찰옥수수를 중 저온에서 장기간 저장할 수 있는 방법을 개발하기 위하여 찰옥수수를 증자하여 진공포장한 후 감마선 조사하여 여러 온도에서 저장하면서 미생물의 생육변화 및 이화학적 성질을 조사하였다. 증자 찰옥수수를 감마선 조사한 후 저장전에 총균수는 3 kGy 조사에서 모두 사멸하였고 곰팡이 및 효모는 34 CFU/g을 나타내었으며, 에서 7개월간 저장 후에 총균수 및 효모, 곰팡이는 대조구에서 를 나타내었으나 3 kGy 조사구에서는 총균수가 검출되

저장조건이 딸기 “미녀봉”의 저장 중 품질 및 미생물학적 변화에 미치는 영향을 연구하기 위하여 저장 중 중량감소율, 부패율, pH 및 적정산도, 총균수 및 곰팡이와 효모 수의 변화가 측정되었다. 딸기 시료는 LDPE로 포장하여 4와 2에서 각각 저장하였다. LDPE포장은 대조구에 비해 중량감소율 및 부패율을 감소시켰다. 또한 포장방법은 저장중 미생물학적 변화에 영향을 끼쳤는데 저장온도와 상관없이 대조구와 비교해서 효모와 곰팡이뿐만 아니라 총균수의