노로바이러스는 전 세계적으로 산발적인 발병 관련 비세균성 위장염의 주요 원인 물질이다. 노로바이러스 검출을 위해 역전사 실시간 PCR (RT qPCR)이 그 민감도와 특이성으로 인해 주요 수단으로 빠르게 자리 잡았다. 그러나 RT qPCR 분석을 위해서는 정확한 바이러스 RNA 추출 방법이 필수적이다. TRIzol 시약은 생물학적 물질로부터 RNA의 추출에 이용되고 따라서 노로바이러스 RNA 추출에도 널리 사용된다. 이 연구에서는 인체 노로바이러스 유전체 그룹 I (GI) 및 유전자 그룹 II (GII)와 생쥐 노로바 이러스(GV) 중에서 GII로부터의 TRIzol 을 이용한 바이러스 RNA의 추출률이 바이러스 시료 용액의 pH에 의존했다는 내용이 다루어졌다. 실시간 PCR의 Ct값으로 비교 한 RNA 추출 수율은 산성 영역보다 알칼리성 pH에서 높았다. 이 연구 결과로 부터 TRIzol을 이용하여 GII RNA 를 추출하여 노로바이러스를 정량적으로 분석할 때 pH조 건이 대단히 중요하다는 것을 알 수 있었다.

The purpose of this study was to obtain data on the contribution of job characteristics (work, autonomy, reward, community, fairness, value) towards job burnout (emotional exhaustion, cynicism), job engagement (vigor, dedication, absorption) and turnover intention in school foodservice employees. Typically, 594 school foodservice employees in the Busan area participated in our survey. Workload (β=－0.512, p<0.001) had the highest negative influence on emotional exhaustion, whereas reward (β=－0.216, p<0.001) and community (β=－0.214, p<0.001) had the highest negative influence on cynicism. Community (β=0.305, p<0.001) and workload p<0.001) had the highest positive influence on vigor. Community (β=0.261, p<0.001) and (β=0.238, p<0.001) had the highest positive influence on dedication. (β=0.287, p<0.001) and community (β=0.224, p<0.001) had the highest positive influence on absorption. Workload (β=－0.195, p<0.001) and community (β=－0.182, p<0.001) had the highest negative influence on turnover intention. Overall, the results show that job characteristics are very important factors affecting foodservice employee’s burnout, engagement, and turnover intention. So should try to reduce the employees’ workload and increase the rewards for them along with activating communication among each other. Key words: school foodservice employee, job characteristic, burnout, engagement, turnover intention

This study investigated the effect of various freezing and thawing conditions on the quality characteristics of radish. White radish was blanched and cooled down to corresponding freezing temperatures of -20℃, -40℃ and -60℃ and, thereafter, the radish was finally frozen in a -20℃ refrigerator for 24 h. The frozen radish was thawed at 4℃, 25℃ and with running water, radio-frequency thawing machine. As quality parameters, pH, drip loss, hardness, color, and sensory evaluation were evaluated. No effect of freezing and thawing conditions on color, pH, and total bacterial count was observed for the samples. Samples that underwent immersion freezing (-60℃) had lower drip loss and higher hardness than samples in other freezing conditions. Sensory evaluation showed that color, hardness, and overall acceptability increased with a decrease in freezing temperature. These results indicate that the temperature of freezing affects the quality of radish.

L. monocytogenes는 Listeriosis를 일으키는 중요한 식중독 균으로 현재 국내 식품공전에서는 증균배양을 기초로 검출하며, 규격은 불검출로 관리하고 있다. 그러나 Listeria 종 간의 혼합오염시 증균 과정에서 경쟁생육이 존재하여 L. monocytogenes 위음성의 가능성이 있다고 보고되고 있다. 국내 식품공전은 L. monocytogenes 증균을 위한 1차 배지로 규정되어 있으나 LEB 배지에서의 Listeria 종 간의 생육 연구는 보고된 바 없다. 본 연구는 식품에서 주로 검 출되는 Listeria 속 4종(L. monocytogenes, L. innocua, L. ivanovii, L. seeligeri)을 LEB배지에 혼합배양하며 증균과 정에서 생육의 차이가 존재하는 것을 확인하였다. 특히, L. innocua에 의해 L. monocytogenes의 생육이 저해되며, L monocytogenes가 L. innocua보다 초기균수가 2.0 log CFU/ mL 이상 오염이 되어있어야지만 L. innocua보다 생육이 잘 되는 것을 확인하였다. Listeria 종 간의 혼합오염이 있 을 경우 현재 검출법으로는 L. monocytogenes의 검출이 어려울 수 있다고 판단된다. 따라서 L. monocytogenes 검출 율을 높이는 새로운 증균배지 개발의 필요성을 확인하였다. 향후 본 연구는 L. monocytogenes 검출률을 높여 국내 식품의 식품 안전에 기여 할 수 있으며 국내 식품 관리 규격 개정 시 기초가 되는 참고 자료로 활용 할 수 있을 것으로 생각된다.

Pumpkin (Cucurbita moschata Duch.) seed is rich in protein and sulfur-containing amino acids. Tofu is a protein gel made from soybean, which is rich in lysine but lacking in sulfur-containing amino acids. This study was conducted to investigate the use of pumpkin seeds in tofu manufacture and to determine its quality and texture characteristics. Soybean was substituted with pumpkin seed to obtain pumpkin seed tofu at the following ratios: 10%, 30%, 50%, and 70% (P10, P30, P50 and P70). Tofu manufactured only with soybean was used as a control (Con). The higher rate of pumpkin seed substitution significantly decreased the moisture content and yield rate (p<0.05). In contrast, pH value and turbidity were significantly increased with the increase in the amount of pumpkin seed (p<0.05). The L-value (81.74~79.04), a-value (－0.19~－3.89) and b-value (12.40~9.84) of samples significantly decreased with the amount of pumpkin seed (p<0.05). No significant difference in syneresis was found among the samples (p<0.05). The hardness tended to decrease with the increase in the amount of pumpkin seed. The microstructure analysis revealed that the pore size of pumpkin seed tofu was smaller than that of Con. These results suggest that the pumpkin seed protein is a useful ingredient in the manufacture of tofu. Increasing the pumpkin seed substitution levels improves the texture of tofu.

This study was conducted to propose the conditions of controlled atmosphere (CA) storage suitable for the storage of winter Chinese cabbage and to investigate the maximum storage period of Chinese cabbage based on the results of the quality analysis for CA storage. The weight loss rate of Chinese cabbage stored at 194 days in low temperature storage was about 2 times higher than that in CA storage. The trimming loss rate of Chinese cabbage stored at low temperature was 1.5 times higher than that of CA storage after 83 days of storage. Bolting of Chinese cabbage was observed in only low temperature storage. The Chinese cabbage firmness of CA storage was not different in the range of 5.7-6.2 N, while low temperature storage gradually increased after 83 days of storage and was 7.5 N at 194 days. The incidence of mold occurred after 83 days in low temperature storage, while in CA storage it occurred after 153 days. In the quality evaluation, CA storage for Chinese cabbage was rated 1.5-1.9 points higher than the low temperature storage. The maximum storage period of Chinese cabbage at low temperature storage was 83 days and that at CA storage 153 days.

본 연구는 소의 가식부위(근육, 신장, 간장, 지방) 중에 서 세팔렉신을 효과적으로 정량분석하기 위한 LC-MS/MS 법을 확립하고 이를 검증하기 위해 수행되었다. 확립된 LC-MS/MS에 대해 특이성, 검출한계, 정량한계, 정확도 및 정밀도에 대한 검증을 통하여 유효성을 확인하였다. 표준 용액을 이용하여 검량성을 작성한 결과, r2 > 0.999 이상의 직선성을 나타내었으며, 세팔렉신에 대한 검출한계와 정량한계는 각각 2~10과 6~30 μg/kg으로 나타났다. 또한, 회 수율은 83.9~106.8%로 나타났으며, 상대표준편차는 2.3~ 14.8%로 나타나 정확성이 우수하였다. 이는 식품의약품안 전처의 잔류동물용의약품 분석법에서 제시한 기준에 모두 적합한 수준이었다. 따라서 본 연구를 통해 개발된 LCMS/ MS법은 향후 소의 가식부위 중 세팔렉신을 분석하는데 효과적으로 활용될 수 있을 것으로 사료된다.

The purpose of this study is to examine the feeding and nutritional status of enteral tube-fed elderly patients. Subjects included 77 elderly hospitalized patients who had received enteral nutrition more than one week before admission. Medical records on admission and actual feeding volume were used to assess anthropometric, biochemical, and nutritional status. Most patients manifested disorders of the nervous system (93.5%) and the average duration of tube feeding was 13.9 months. The average feeding volume of formula was 1,107 mL per day and the mean ratios of calorie and protein (supplied vs. required) were 81.7% and 80.9%, respectively. At admission, 57.4% of the patients were malnourished according to the institutional criteria. Patients receiving less than 80% of the required calories were in worse nutritional status compared with those receiving more than 80% of the required calories. Body mass index, percent ideal body weight, serum albumin level and blood lipid levels (total cholesterol, HDL-cholesterol, triglyceride) were significantly lower in patients receiving less than 80% of the required calories. These results indicate the high prevalence of malnutrition and the need for increased attention and nutritional care of elderly patients undergoing long-term enteral nutrition.

Salmonella는 전세계적으로 식중독을 유발하는 주요 원 인 균으로서, 식중독을 유발하는 Salmonella를 신속하게 검출하는 방법은 식품 안전을 위한 중요한 도구이다. Realtime PCR은 식중독균을 검출하기 위한 신속검사법으로 널 리 사용되어 왔다. 최근에는 NBS LabChip real-time PCR 이라는 새로운 시스템이 칩타입으로 조작이 간편하며 초 고속의 real-time PCR 시스템이라는 보고가 있었다. 본 연 구에서는 살모넬라의 신속한 검출을 위하여 NBS LabChip real-time PCR에 기반하여 real-time PCR 반응 시간이 20 분 이내인 검출법을 확인하고자 하였다. 프라이머와 프로 브 설계를 위해 두 개의 타겟 유전자(invA, stn)가 선택되 었으며, 특이도와 민감도(검출한계)를 평가함으로 개발된 검출법을 검증하고자 하였다. 본 연구에서는 특이도 검증을 위해 Salmonella 균주 42주와 Non-Salmonella 균주 21 주를 포함하였으며, 본 방법으로 Salmonella 42주에 대해 서만 정확하게 검출이 가능하였다. 검출한계는 살모넬라 genome DNA 기준으로 101 copies/μL 였으며, 소시지에서 는 4시간 증균 이후 접종균수로서 101CFU/g 에서 102 CFU/ g까지 검출이 가능하였다. 본 연구에서 개발된 검출법은 신속한 식중독 원인조사에 활용될 수 있을 것으로 기대된다.

This study aims to investigate the physicochemical properties of extruded Biji. As the extrusion process variables, the barrel temperature and the moisture content were adjusted at barrel temperatures of 120, 140, and 160°C and 35, 45% respectively. L-value (lightness) increased as the moisture content and barrel temperature increased from 35% to 45% and 120°C to 160°C. In contrast, decreasing a-value and b-value resulted in increasing moisture content and barrel temperature. Total sugar, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity, and total phenolic compound were increased by the extrusion process. The water solubility index (WSI) and dietary fiber of the extruded biji decreased to 3.9% and 592.6 mg/g compared to the non-extruded biji of 12.3% and 592.6 mg/g. According to the result of this study, the extruded biji could be used in various kinds of food processing, and future study is needed to find the optimum condition of the extrusion.

피카오프레토 등 복합물의 개별인정 시험법인 총 폴리 페놀 시험법에서 Folin-phenol 시약 선정, 발색시약인 35% 탄산나트륨의 반응시간 및 온도, 시료량 등을 비교 실험 하여 총 폴리페놀에 대한 표준화된 시험법을 확립하였다. 총 폴리페놀의 표준화된 시험법의 밸리데이션을 수행한 결과 회수율은 102.3~112.4%, 정밀도의 표준편차는 0.81~ 3.18%였으며 검량선의 직선성은 결정계수(r2)가 0.999이상 을 나타냈고 검출한계는 5 μg/mL, 정량한계는 15 μg/mL 이었다. 유통제품에 대해 표준화된 총 폴리페놀 시험법의 적용성 검토를 위해 1건의 검체를 수거하여 분석한 결과, 총 폴리페놀 표시 함량 대비 105.6%로 적합하였다.

This study was conducted to investigate the quality of pumpkin paste added with different starches during storage at 4℃ for 8 days. Pumpkin paste was evaluated for pH, carotenoid, syneresis, color values, texture, and sensory characteristics. The properties of the pumpkin paste were studied on three different starches (CON: control, MCP: cow pea starch, MMB: mung bean starch, and MSP: sweet potato starch). The initial pH of the pumpkin paste with starches were 6.13~6.16. The pH of pumpkin paste increased as the starches added increased. The carotenoid content of CON was higher in the pumpkin paste processing. The change of syneresis significantly increased with the storage period, and the amount of change of MCP and MMB added with starch was smaller than CON without the added starch. The Hunter's L-values of the pumpkin paste increased, whereas the a- and b-value decreased as the amount of starches added increased. The results of the textural analysis showed that the hardness of MCP and MMB was different from that of other pumpkin paste, showing a lower value such as 38.26 g, 38.93 g, while CON and MSP was 40.43 g and 42.49 g, respectively. A sensory evaluation indicated that starches could enhance the overall texture characteristics of pumpkin paste. In terms of the overall acceptance of the pumpkin paste, the experimental group with MCP scored the best.

This study investigated the effects of blanching time (1-5 min), types of blanching solution (1% NaCl vs 1-3% sucrose), addition of packaging with blanching solution, and thawing methods (thawing in packaged versus depackaged units) on the qualities of frozen Aster scaber. As quality parameters, drip loss, moisture content, shear force, color, and sensory test were conducted. Aster scaber was blanched in 1% sucrose for 4 min and packaged with the same solution prior to freezing whereas frozen Aster scaber exhibited palatable quality when it was removed from the package and thawed in running water. Consequently, this study demonstrated that the optimized processing condition provided qualities like non-frozen control.

본 연구에서는 농법별 주요 엽채류의 생산단계에서 부터 수확된 농산물의 모의유통과 세척단계까지 동일 개체를 대상으로 미생물상의 변화를 비교하여 단계별 미생물적 안전성 관리방안을 수립하고 개선방안을 도출하고자 수행하였다. 생산 및 유통중 위생지표균 및 식중독 균의 검출량을 조사한 결과 호기성 세균을 제외한 조사대상 미 생물은 발병량 이하로 안전한 수준이었다. 생산단계에서 검출되지 않거나 낮은 수준이었던 위생지표균 및 식중독 균의 검출량은 엽채류의 종류, 재배방법에 관계없이 생산 단계보다 모의유통 중 증가하였으나, 세척단계에서 다시 감소하는 경향을 나타내었다. 생산과정에서 검출되지 않았던 미생물이 모의유통중 검출된 것은 수확후 처리과정 중 교차오염으로 예상되며, 생산단계에서 낮은 수준으로 검출되었던 미생물의 증가는 유통과정 중 증식한 결과로 판단된다. 따라서 유통단계에서는 저온유통시스템의 확대 적용과 운반도구 등에 대한 위생관리 수준을 향상시키기 위한 노력이 필요하다.

A study on the decolorization method of salt-fermented anchovy sauce using activated carbon was carried out. The anchovy sauce filtered with a diatomaceous membrane after heating at 85℃ for 20 min was reacted with 3.0% (w/w) activated carbon with pH 4.5 at 55℃ for 2 hr. The color difference value and turbidity related to the decolorizing effect showed excellent improvement results with a difference of 23% and 88%, respectively. The overall taste and color preference of decolorized anchovy sauce were significantly increased in shrimp sauce by 0.4-0.5 points (p<0.05). In order to minimize the precipitation of amino acid during storage, 1% silicon dioxide or gelatin was mixed and filtered after the activated carbon reaction. Turbidity, as index of sedimentation, was improved by 15% at 30℃ for 2 weeks. The recycle system with activated carbon coated membrane filter reduced the processing time and cost on decolorization of anchovy sauce. When the concentrated anchovy sauce was recirculated, the amount of total protein as an indicator of taste compounds was increased by 125%, which is 1.8% compared to the conventional 0.8%, indicating that it is highly useful as a liquid seasoning.

The aim of this study was to examine the association between egg consumption and the risk of chronic disease in Korean adult females using the 2013 Korea National Health and Nutrition Examination Survey. A total of 1,230 subjects aged 40~64 were classified into the 4 groups according to the number of egg consumed per week: <1, 1~2.9, 3~5, ≥5.1 As egg consumption increased, the intake of energy, protein, fiber, cholesterol, calcium, potassium, riboflavin, and vitamin C increased. The percentage of the subjects with lower intake of energy, protein, calcium, iron, vitamin A, riboflavin, niacin, and vitamin C than the estimated average requirement in the <1 group were the highest among the groups. The blood lipid profile including total cholesterol and LDL-cholesterol was not significantly different among the 4 egg groups. The higher egg consumption was inversely related to a lower odds ratio of metabolic syndrome, hypertriglycemia, hyperglycemia, hypoHDL-cholesterolemia, and hypertension. This result indicates that egg consumption does not elevate the plasma cholesterol level and has a beneficial effect of decreasing the risk of chronic disease. (175)

본 연구는 시설엽채류에서 재배농법별 미생물학적 안전성을 평가하기 위해서 깻잎과 상추를 대상으로 수행하였다. 유기농 및 관행 농가로부터 생산 및 수확 단계에서 식물체, 수확장갑, 수확비구니, 토양피복재 등으로부터 총 2,304개의 시료를 채취하여 Total aerobic bacteria, Coliforms, E.coli, Environmental Listeria, Yeast & mold 등의 위생지 표세균과 Staphylococcus aureus, Bacillus cereus, Salmonella spp., Clostridium spp., L. monocytogenes등의 병원성미생물을 분석하였다. 시설엽채류의 생산과정에서는 재배농법에 상관없이 위행지표세균은 검출되지 않거나 3.4 Log CFU/ 100 cm2 이하로 검출되었다. 유기농법으로 생산되는 깻잎 과 상추에서 B. cereus와 S. aureus가 0.22~1.55 Log CFU/ g로 조사되었고, 관행농법에서는 S. aureus는 검출되지 않았으며 B. cereus는 0.42~2.19 Log CFU/g으로 조사되었으나 통계적 유의차는 없었다. 수확도구 및 멀칭필름에서도 재배농법과는 관계없이 위생지표세균과 유해균의 오염도는 낮았으며 차이는 없었다. 그러나 수확도구에서의 미생물 오염도가 높아질수록 식물체 표면의 미생물 오염도도 높아지는 정의 상관관계(R2 = 0.4526)가 있었다. 또한 유기농 시설엽채류 생산시 토양 피복시 위생지표세균과 병원 성미생물이 검출되지 않거나 피복을 하지 않은 경우에 비하여 낮은 경향을 나타내었다. 본 연구결과, 시설엽채류 생산시 재배농법의 차이보다는 토양피복 및 수확과정의 미생물적 위생관리가 더욱 필요함을 알 수 있었다.

In this study, the quality characteristics of cheonggukjang with addition of different quinoa were investigated. We evaluated the quality and sensory characteristics of cheonggukjang, including the pH, amino nitrogen, slime contents, color value, and total aerobic bacteria. Moreover, The anti-oxidant activities were measured as total polyphenol content, 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and superoxide dismutase (SOD) activity. The pH and amino nitrogen content were noted to significantly increase during the fermentation period in all the samples. The slime content of cheonggukjang added with quinoa increased with increased fermentation time, but cheonggukjang with addition of 20% of quinoa was decreased. The L value and b value decreased significantly with increased fermentation time, but the a value increased significantly. The microbial tests of cheonggukjang with addition of quinoa showed that the aerobic micro-organisms count was 7.45~9.10 Log cfu/g. Total polyphenol contents increased in all groups during the fermentation period, and activity increased with an increased percentage of added quinoa. The DPPH radical scavenging activity and SOD-like activity of cheonggukjang (with addition of quinoa) were also significantly higher than those of the control. The sensory quality of quinoa 10% cheonggukjang was stronger in flavor, and taste, and demonstrated a higher level of overall acceptability, when compared to the other groups.

본 논문에서는 유통 농산물 잔류농약 부적합 현황 분석을 위하여 네트워크 분석 기법을 적용하였다. 분석용 데이터는 『2017년도 식품안전관리지침』내 “유통농산물별 잔류농약 부적합 현황”을 참고하였으며, 주요 분석 기법 으로는 중심성 분석(연결정도 중심성, 근접 중심성, 매개 중심성)을 적용하였다. 연결정도 중심성 분석결과 chlorpyrifos와 diazinon에서 “연결된 노드”가 가장 많은 것으로 확인되었다. 근접 중심성에서는 19종을 제외한 농약성분들 사이에서 유사한 정도를 보여주고 있었다. 매개 중심 성 분석 결과에서는 fluioxonil과 chlorpyrifos가 가장 높은 경향을 보여주어 이들이 농약성분 네트워크에서 “가교” 역 할을 수행 하는 것으로 파악되었다. 네트워크 분석 결과, 기존의 통계분석을 통해서는 분석 할 수 없었던 농약성분 들 사이의 “관계” 데이터를 확인 할 수 있었다. 향후 이러한 분석기법은 최적화/정교화 과정을 통해서 보다 정밀하게 농약성분 부적합 현황 분석을 위한 도구로서 적용되리라 기대한다.

The objective of this study was to compare physicochemical, microbial, and antioxidant properties of domestic and imported wheat kernels for bread making. Two domestic (JK1, 2) and three imported (ND, DNS, and CWRS) kernels were compared. Domestic kernels had higher moisture contents, and lower ash and protein contents (p<0.05). In grain characteristics, JK1 had 13.62% of damaged kernels, which was the highest among the samples (p<0.05). JK2 was similar to imported kernels in the ratio of sound kernels, foreign materials, and damaged kernels. Kernel size of JK1, 2 was larger than the imported kernels; therefore, kernels area and perimeter were higher by the image analyzer. Domestic kernels hid lower total aerobic counts the imported kernels (p<0.05). Domestic kernels and DNS had no yeast, while NS and CWRS had yeast in kernels. DNS (3.08 mg gallic acid equivalent (GAE)/g) had the highest total polyphenol content (TPC), followed by JK1 (2.81 mg GAE/g). JK2 had the lowest amount of TPC as 2.26 mg GAE/g. Total flavonoid content (TFC) was the highest in DNS as 0.44 mg catechin equivalent (CE)/g and JK2 was the lowest as 0.12 mg CE/g. Domestic wheat kernels had lower protein content and lightness than the imported wheat kernels so that flour from domestic wheat kernels may have lower quality for baking.