This study was performed to investigate the effects of garlic on uncoupling protein 2 (UCP2) transcriptional regulation of UCP2- luciferase transgenic mice fed on a high fat diet to induce obesity. To examine the transcriptional regulation of UCP2, we generated transgenic mice with a UCP2 promoter (-1,830/+30 bp) containing luciferase as a reporter gene. UCP2-luciferase transgenic mice were fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (TG-CON), or high-fat diets supplemented with 2% (TG-GL2) or 5% (TG-GL5) garlic for a further 8 weeks. Dietary garlic reduced body weight and energy efficiency ratio in the TG-GL5 group, compared to the TG-CON group. Furthermore, garlic supplementation significantly decreased white adipose tissue fat mass and plasma levels of triglycerides, total cholesterol, and leptin in the TG-GL2 and TG-GL5 groups, compared to the TG-CON group. Specifically, UCP2 promoter activity in metabolic tissues such as liver, white adipose tissue, brown adipose tissue, and skeletal muscle was increased by garlic supplementation. These results suggest that dietary garlic was partially associated with an increase of UCP2 transcriptional activity in metabolic tissues for decreasing obesity.

King oyster mushroom (Pleurotus eryngii), an improved species of oyster mushroom, is a popular ingredient in Asian cuisine. Spleen cells were treated with various concentrations (0, 5, 10, 50, 100, 250, 500, and 1,000 μg/mL) of king oyster water extracts (KOWE); then, the proliferation of the cells was measured 24, 48, and 72 h after each treatment. Also, type 1 T helper cytokine productions (TNF-α, IFN-γ, and IL-2) were measured in activated macrophage by KOWE in seven concentrations. Under the condition of its 50, 100, 250, and 1,000 μg/mL for 48 h, the proliferation of cells was increased. However, there was no significant fluctuation in the spleen cells proliferation for 24 and 72 h-long KOWE exposure. To determine cytokine (TNF-α, IFN-γ, IL-2) productions of type 1 T helper cells, macrophage was stimulated by KOWE for 48 h. Treatment of KOWE gave a rise to the levels of TNF-α and IFN-γ, but not in that of IL-2 productions. These results suggest that king oyster mushroom water extracts may be beneficial for enhancing immune functions in its high concentration.

An investigation was conducted to evaluate the hygienic status of 53 high school foodservice systems in Gyeonggi province by using hygiene management guide checklist, ATP bioluminescence assay of food utensils were conducted during process. The 5 hygiene management guide checklist groups about personal hygiene, cooking facilities control, cross contamination control, cook and storage control, management control were checked by experts and had good grades but there were some inadequate behaviors on observation. Total cleaning levels were inadequate, including hand, rubber gloves, aprons, knives, food tray, machine and instruments. The possibility of cross contamination is also noted in handles for refrigerators, ovens, food dryers, hand washing. It was also noted that there were too much work on the nutritionist and cook, additional personnel need to be added. lack of space, deterioration of facilities were identified in some high school foodservice systems. ATP bioluminescence assay was conducted on surface of food facilities, ATP ranged 1,393±5,041.2 RLU on yellow gloves, 244±258.7 RLU on pink gloves, 3,780±11,418.6 RLU on apron, 49,056±62,831.4 RLU on refrigerator grip, 41,422±61,259.8 RLU in oven, 31,407±41,344.9 RLU on hand cleaning board.

Plantago asiatica L., observed frequently in East Asia, is a known herb used in traditional medical remedies several studies report that P. asiatica L has anti-inflammatory and antioxidant effects. To determine the production of cytokines (IL-2, IFN-γ, and TNF-α) induced by lipopolysaccharide (LPS) and non-LPS-stimulated macrophages, an ELISA assay was conducted using cytokine kits. Mice splenocytes were cultured for 48 h with various concentrations of P. asiatica L. (5, 10, 50, 100, 250, 500, and 1,000 μg/mL) or with mitogens (ConA or LPS). P. asiatica L. increased the proliferation of mice splenocytes, especially under the condition of its concentration ranging from 250 to 1,000 μg/mL. In addition, Plantago asiatica L. notably induced cytokine production of (IL-2, IFN-γ, and TNF-α) at its concentration of 250~500 μg/mL. These results suggest that supplementation with P. asiatica L. water extracts may play a potential role in enhancing immune function by mediating splenocyte proliferation and cytokine production through its anti-inflammatory activit.

This study investigated the quality characteristics and antioxidant activities of gluten-free cookies containing teff flour. By substituting 0% (control), 25% (TF25), 50% (TF50), 75% (TF75), and 100% (TF100) of wheat flour with teff flour, five samples were produced. Baking loss rate was the highest in TF25 at 13.76% and the lowest in TF75 at 4.03%. Spread factor was significantly higher in cookies made with teff flour (83.00~85.00) than in the control (81.33) (p<0.05). There was no significant difference in density among the samples at 1.17~1.25 g/mL (p<0.05); however, pH significantly decreased at 6.42~6.04 (p<0.05). While the L-value and b-value significantly decreased with the amount of teff flour, the a-value significantly increased (p<0.05). The ΔE value was the highest in the control at 31.31 and the lowest in TF100 at 58.69. Hardness was the highest in the control at 42.04 N than in cookies containing teff flour. The content of polyphenols was the highest in TF100 at 3.37 μg GAE/mg and the lowest in the control at 1.32 μg GAE/mg. The content of flavonoids was the highest in TF100 at 3.66 μg QE/mg and the lowest in controls at 0.45 μg QE/mg. The value of DPPH IC50 was the highest in the control at 3,723.00 μg/mL and the lowest in TF 100 at 405.27 μg/mL. The value of ABTS IC50 was the highest in the control at 1,822.32 μg/mL and the lowest in TF100 at 529.30 μg/mL. In sensory evaluation, while control, TF75, and TF100 had a higher score in appearance at 5.52~5.60, all samples had no significant differences in flavor, sweetness, savory taste, chewiness, and overall acceptability (p<0.05). These results showed that the gluten-free cookies containing teff flour can improve the quality characteristics and antioxidant activities of a cookie. We concluded that gluten-free cookies containing 100% teff flour are desirable.

Sweet potato-malt worts were prepared by using sweet potato paste of Shinyulmi and Shinjami as the main adjunct, enzymes, malt, hop, and water. We brewed low-malt beers of the lager- or ale-type by using these worts and inoculating bottom and top fermenting yeast, respectively. Moreover, the componential and functional characteristics of the resulting beers were evaluated. During saccharification of sweet potato, the addition of an enzyme agent containing α-amylase caused an improvement in filterability and an increase of total sugar. The sugar content of sweet potato-malt wort which was prepared by the addition of 0.1% enzyme agent containing α-amylase and a three-step infusion procedure was 13.5 °Brix adequate for beer brewing. The polyphenol and anthocyanin contents of Shinjami beer were increased with increasing content of the paste, and also increased DPPH and ABTS radical scavenging activities. But in Shinyulmi beer it were decreased. A strong correlation was obserbed between antioxidave activities and polyphenol and anthocyanin contents of sweet potato beers. In all lager- and ale-type low-malt beers using 41.6% of Shinjami pastes, sensory attributes very similar to those of 100% malt beer were obtained and they were very good as they had unique red color.

This study was carried out to analyze the pasting and antioxidant characteristics of cooked mixed grains consisting of germinated millet and rice with varying addition rates and cooking methods. In this study, we chose two foxtail millets: glutinous (‘Samdachal’) and non-glutinous (‘Samdame’), along with a glutinous proso millet (‘Ibaekchal’). Cooked mixed grain rice-added germinated millet was cooked by general and high pressure cooking methods, with and without fermented alcohol. The peak and trough viscosity of germinated millet were decreased significantly with increasing amounts of germinated millet. The phenolic compounds and radical scavenging activity of cooked rice-added germinated millet increased significantly with the addition of germinated millet (p<0.05). The total polyphenol contents of cooked rice-added germinated Samdachal, Samdame, and Ibaekchal were 127.74~194.11, 128.20~192.56, and 128.88~171.28 μg GAE/g, respectively. The total flavonoid contents were 13.79~30.23, 14.77~25.99, and 15.28~29.56 μg CE/g, respectively. DPPH and ABTS radical scavenging activities were 3.31~5.73 and 6.31~9.69 mg TE/100 g for cooked rice-added germinated Samdachal; 3.14~8.58 and 6.05~10.19 mg TE/100 g for Samdame; and 3.37~7.45 and 6.27~9.27 mg TE/100 g for Ibaekchal, respectively. In this study, phenolic compounds and radical scavenging activity of cooked mixed grain rice-added germinated millet with different addition rates and cooking methods can be used as basic data for manufacturing processed products.

The purpose of this study was to investigate the fad dieting status and needs of nutrition education on healthy dieting in male and female collegians according to the desired loss weight. The subjects were 611 students (287 males, 324 females) from universities in Gyeonggi and Gangwon provinces in Korea. The subjects were divided into the following four groups based on the difference between their current body weight and their desired body weight: no desired weight loss (WL0), desired weight loss less than 5 kg (WL5), desired weight loss 6 to 10 kg (WL10), and desired weight loss more than 11 kg (WL11). The fad dieting status was surveyed using a questionnaire about 11 selected trendy dieting methods. The higher desired weight loss group showed higher current weight and BMI in both males and females. Self weight overestimation was higher in the females than in the males, and in the WL10 group than in the other groups of males. As desired loss weight increased, weight loss trials and experienced trendy dieting types increased in both males and females. In the male subjects, the experience of the half meal diet and the chicken breast diet was significantly associated with the desired loss weight. In females, trials of more trendy diet types including the half meal diet, cereal diet, fasting, and Atkins diet were significantly associated with the higher desired loss weight. The need for nutrition education on healthy dieting was high among all the different desired weight loss groups, showing that a minimum of 60% of subjects in each group answered ‘necessary and strongly necessary’, with the highest in the WL11 group in both males and females. From these results, desired loss weight is associated with higher current weight status, unhealthy weight control practices, and needs for nutrition education in both male and female subjects. Therefore, in the future, nutrition education programs should give greater attention to providing healthy dieting methods for young collegians who desire more weight loss.

The qualities of rice cooked on heat plate, induction heat and heat plate with pressure, were investigated. The weight, volume, water soluble index (WSI), hydration by SEM (Scanning Electron Microscope), and gelatinization by DSC (Differential Scanning Calorimetry), as well as the consumer acceptability of cooked rice were analyzed. The weight, volume and WSI of rice cooked on heat plate with pressure were higher than those of rice cooked on heat plate and induction heat. The rice cooked on heat plate with pressure also showed higher degree of hydration and gelatinization, and lower degree of enthalpy of gelatinization than the rice cooked on heat plate and induction heat for 5~15 min. The consumer acceptability revealed that the odor, appearance, taste, texture and overall acceptance of rice cooked on induction heat were better than those of rice cooked on heat plate and heat plate with pressure. During storage in a cooker for 0~12 h, there was a decrease in the consumer acceptability of cooked rice. Overall results indicate that the qualities of rice cooked on induction heat and heat plate with pressure were higher than those of rice cooked on heat plate.

Ganoderma lucidum has been traditionally used as a medicine for treatment of bronchitis, arthritis, and high blood pressure, and it has been reported to display many biological activities including anticancer and immune activities. Since mushroom mycelium is known to have excellent biological activities together with mushroom fruiting body, studies on biological activities of mushroom mycelium have been actively conducted. Thus, the present study compared the biological activities before and after the cultivation of Ganoderma lucidum mycelium on Atractylodes rhizoma. When the radical scavenging activity was assessed by the DPPH assay, ARGL (ethanol extract of Atractylodes rhizoma mycelium fermented with Ganoderma lucidum) showed radical scavenging activity of 5.58~82.56% at concentrations of 10~500 μg/assay, while AR (ethanol extract of Atractylodes rhizoma) showed radical scavenging activity of 5.27~72.08% at the same concentrations. When measured by using the ABTS assay, ARGL showed higher radical scavenging activity than AR, which was consistent with the result obtained by the DPPH assay. In the MTT assay, the cytotoxicity of ARGL against all cell lines was higher than that of AR. In particular, the cytotoxicities of AR and ARGL against Hep3B at a concentration of 400 μg/assay were 71.81% and 86.40%, respectively. In addition, the result obtained by the SRB assay was consistent with the result obtained by the MTT assay. According to the results mentioned above, there is a high probability that medicinal herb cultures using mycelium can be used as sources of functional foods since the cytotoxicities against cancer cells and antioxidant activities increased when the mycelium was fermented with Atractylodes rhizoma.

In this study, we conducted an empirical analysis using structural equation modeling (SEM) by distributing questionnaires to 208 Chinese tourists who constitute the largest proportion of foreign tourists visiting South Korea. The survey was conducted in a face to face (FTF) manner with the aim to contribute to globalization of Korean cuisine through comprehensive analysis of the effects of preference characteristics of Korean wave dramas on the relationship among images, attitudes, and purchase intentions for Korean cuisine. The main actor characteristics among the preference characteristics of Korean wave dramas had a significant effect on the attitudes and purchase intentions for Korean cuisine. However, the thematic characteristics among the preference characteristics of Korean wave dramas did not have a significant effect on the purchase intentions for Korean cuisine; in addition, the production characteristics did not have a significant effect on the attitudes and purchase intentions for Korean cuisine. The eco-friendly and health images of Korean cuisine had a significant effect on the purchase intentions for Korean cuisine, and the attitudes toward Korean cuisine significantly affected the purchase intentions for Korean cuisine. Based on the results of this study, it is considered necessary to continuously publicize Korean cuisine through Korean wave dramas to build positive attitudes toward Korean cuisine through enhanced images of Korean cuisine.

This study investigates the quality characteristics of sweet persimmon jam added with aronia juice. Sweet persimmon jam was prepared with sweet persimmon, sugar, pectin and various amounts (20, 40, 60, 80%) of aronia juice, and it was assessed for pH, total acidity, color, sensory evaluation and hydrogen donating ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging effects and content of the total polyphenols, respectively. Increasing the amount of aronia juice decreased the pH, whereas the total acidity gradually increased. In addition, with an increased amount of aronia juice, the lightness (L) and yellowness (b) significantly decreased, while redness (a) gradually increased. By increasing the amount of aronia juice, the polyphenol contents, DPPH and ABTS radical scavenging activities were increased. The results of the sensory evaluation showed that the sweet persimmon jam added with 80% aronia juice had the highest overall-acceptability scores. The results suggest enhancing the quality, palatability and acceptability of sweet persimmon jam by adding 60~80% of aronia juice.

The objective of this study to investigate the satisfaction of elementary and middle school students in Chungnam province. We surveyed related to school food service, concerns about food ingredients and dietary life, perceptions of free school food service and satisfaction the quality and management of the school food service. Furthermore, this survey with the previous. Compared to year, frequency of education related to school food service and concerns about food ingredients and dietary life. Perception on free school food service increased 1.2%, not statistically significant. Satisfaction for the quality of the school food service statistically significant increase compared to 2015. ore individuals educated the foods’ countyof- origin and a higher concern the food materials and dietary life, subsequently higher satisfaction for the quality and management of school food service. The results of this study suggest that concern and concentration on software of school food service such as education and guide are important.

The study subjects were 302 adult males and females aged more than 20 years living in the metropolitan area of South Korea. This study was conducted to obtain baseline data to establish proper development and marketing strategies by examining the effects of food-related lifestyles on the importance of diet, purchasing behavior towards diet lunch boxes, and their selected attributes such as menu, packaging, and services. With respect to food-related lifestyle, a cluster analysis was performed by using five factors such as convenience factor, health factor, safety factor, taste factor, and economy factor obtained from factor analysis to derive the economy type, the taste and economy type, the convenience type, the safety type, and the health type. As a result, the respondents regarded 'food hygiene (4.59)', 'freshness (4.47)', 'taste (4.28)', and 'nutrient balance (4.19)' as the selected attributes of diet lunch box menus. Moreover, the importance of diet lunch box menus (β=0.179) was increased with increasing safety orientation. 'Shelf life label (4.42)' was the most important selected attribute of diet lunch boxes, followed by 'ingredient label (4.19)', 'nutrition facts label (4.16)', and 'indication of origin (4.15)'. In particular, the importance of packaging for diet lunch boxes (β=0.203) was increased with increasing safety orientation. With respect to the selected attributes of services in purchasing diet lunch boxes, 'provision of personalized menus (4.07)' was the most important, and the importance of services for diet lunch box (β=0.160) was increased with increasing taste and economy orientation. Based on the above results, the respondents gave importance to the selected attributes related to food safety and health such as hygiene and, freshness. In addition, they also placed emphasis on hygiene and safe factors such as shelf life, ingredients, and nutrition facts labels. Therefore, it is considered necessary to develop diet lunch boxes by taking these factors into account. Furthermore, in services for diet lunch boxes, it is considered necessary to establish a service system capable of providing consumers with specialized menu or nutrition counseling according to the food-related lifestyle for their proper health management. Particularly, because consumers place emphasis on both food hygiene and safety, and health, it is considered necessary to thoroughly manage hygiene, safety, and nutrition in menu or packaging so that it is possible to enhance customer satisfaction by considering these selected attributes in greater detail.

치자(Gardenia jasminoides Ellis fructus, GJE) 씨에서의 70% 에탄올, CM (chloroform: methanol, 부피 비 2:1(CM)) 및 노르말 부탄올 추출 용매별 flavonoid 함량 및 항산화 능, 금속 킬레이 트 능력 측정을 통하여 치자 씨의 기능성 식품 소재로서의 가치를 검토한 결과, 치자 씨의 proanthocyanidin 함량은 건조 무게(g) 중 카테킨(catechin) 당량으로 계산하여 70.035±0.772 mg으로 나타났으며, 추출 수율은 CM (36.39%), 70% 에탄올(27.32%), 노르말 부탄올(26.23%) 순으로 나타났 다. 추출 용매별 항산화 능은 농도(0.2, 0.4, 0.6 mg/mL)가 증가할수록 유의적으로 증가하였으며 (p<0.05), 양성대조군으로 사용된 ascorbic acid, butylated hydroxyanisole (BHA), ethylenediamnetetraacetic acid (EDTA) 보다는 낮은 활성이 확인되었다. Flavonoid 함량(mg)은 시료 g 당 케르세틴(quercetin) 당량으로 계산하여 70% 에탄올(0.830), CM (0.752), 노르말 부탄올(0.105) 순 으로 확인되었으며, 항산화 능 분석 실험과 금속 킬레이트 능력 실험에서도 이와 유사한 양상으로 동정 되어 모든 분석에서 70% 에탄올과 CM 추출물이 강한 생리활성을 나타내었다. 따라서 치자 씨의 용매 별 flavonoid 함량에 따라 항산화 능 및 금속 킬레이트 능력이 증가하는 것으로 관찰되었다. 이에, 치자 씨는 proanthocyanidin과 flavonoid 화합물을 다량 함유하고 있으며, 높은 항산화 능 과 생리활성을 가 지고 있어 기능성 식품 및 천연 항산화제로서의 가치가 있을 것으로 추정된다.

Probiotics may be defined as microbial agents that can benefit humans and have been used primarily by mankind in fermented foods. A representative of probiotics is lactic acid bacteria (LAB), which has received attention recently due to its perceived health benefits. We reviewed research papers about Bifidobacerium and Lactobacillus, which are two of the most known of the LAB. Bifidobacerium alleviates diarrhea from various causes by improving intestinal microflora, and it has been reported that it reduces Helicobacter pylori bacteria known as the cause of gastric ulcer and stomach cancer. Bifidobacerium has also reported to be effective on immunization and allergy, reduce blood cholesterol, cancer, lactose intolerance. Lactobacillus alleviates diarrhea but has not fully demonstrated its effectiveness relative to other health issues. Several lactic acid bacteria and yeast are expected to act as probiotics, so, in the future, more research on the health benefits of lactic acid bacteria is needed.

천연색소는 색을 발현할 수 있는 물질로서 식품, 화장품, 의약품 등에 사용된다. 천연색소는 동・식물에서 추출하여 만들기 때문에 크기가 균일하지 않으며 특히 적색색소의 경우, 다른 색의 색소 에 비해 친유성이 강해 수용액 상태에서 쉽게 응집이 되는 경향이 있다. 또한 응집에 의한 크기변화로 인해 색의 재현이 어려운 문제점이 있다. 적색색소로 기존에 사용했던 동물성 색소인 코치닐 추출 색 소는 알레르기를 유발하고 식용으로서의 거부감이 있는 등의 문제점으로 인하여 사용이 기피되는 추 세이다. 본 연구에서는 적색의 식물성 색소인 라이코펜 추출물과 치자황색소를 사용하였고 Ball-milling을 이용하여 색소의 크기를 균일하게 만든 후 Asymmetrical flow field-flow fractionation(AsFlFFF), Dynamic light scattering(DLS)를 이용하여 크기를 분석하고 색차계를 이용하 여 색을 확인하는 연구를 진행하였다. 실험결과 milling 전에는 색소 입자의 크기가 크고 크기분포가 넓었으나, milling 후에는 크기가 감소하고 크기분포가 좁아짐을 확인하였다. 색차계 측정 결과, milling 시간이 증가함에 따라 밝기, 적색도, 황색도가 높아져서 밝은 적색을 나타내었다.

식물 및 동물성 단백질 유래 펩타이드 형태의 단백질 가수분해물들은 항산화, 고혈압 완화, 면역조절, 진통완화 및 항균작용 등 생리활성이 있는 것으로 알려져 왔다. 본 연구는 건조 해삼으로부터 해삼육 슬러리를 제조하고 flavourzyme 프로티아제를 이용하여 단백질 가수분해 최적공정을 수행하였 다. 이어서 생산된 펩타이드의 항산화 특성을 연구하였다. 효소반응 최적공정은 표면반응 분석법을 이 용하여 수행을 하였고 공정의 범위는 반응온도 40-60 ℃, 반응 pH 6-8, 효소의 농도 0.5-1.5%(w/v) 이었다. 해삼의 단백질 최적 효소가수분해 공정조건은 효소 반응온도 48–50 ℃, 반응 pH 7.0–7.2, 효소 의 양은 1.0-1.1%(w/v)에서 결정 되었다. 이때 단백질 가수분해 수율은 43-45%에 도달하였다. 생산된 대부분 가수분해물의 분자량들은 전형적인 펩타이드인 분자량 500-3,500Da로 분포되었다. 펩타이드들 은 항산화 능력은 금속 킬레이션 능력(IC50, 1.25 mg/mL), DPPH 소거능(IC50, 3.40 mg/mL), 슈퍼옥사 이드 라디칼 소거능(IC50, 10.3 mg/mL), 하이드록시 라디칼 소거능(IC50, 22.11 mg/mL) 순서로 저해농 도가 낮음을 보여 주었다. 따라서 해삼 단백질 가수분해물은 건강 기능 식품소재로서 활용할 가치가 높 을 것으로 기대를 한다.

치자 껍질의 70% methanol, ethyl acetate (EA) 및 distilled water (DW)의 용매 별 추출물 의 total phenol 함량 및 질소 산화물 소거능, 환원력, β-carotene 탈색을 이용한 항산화력 및 지질과 산화 저해능 측정을 통하여 치자의 기능성 식품 소재로서의 가치를 검토한 결과, anthocyanin 함량은 3.519±0.635 mg/100 g DW로 나타났으며, 용매 별 추출 수율은 DW (39.87%), 70% methanol (36.26%), EA (2.88%) 로 관찰되었다. 추출 용매 별 항산화 활성은 농도(0.2, 0.4, 0.6 mg/mL)가 증가 할수록 유의적으로 증가하였으며 control로 사용된 ascorbic acid, BHA, trolox 보다는 낮은 활성이 확 인되었다. 치자 껍질의 total phenol 함량은 EA, 70% methanol, DW 추출물 순으로 EA 추출물에서 26.59±0.20 CAE (caffeic acid equivalents) mg/g으로 가장 높았으며, Nitric oxide (NO) radical 소거 능에서는 70% methanol (70.32~76.15%), DW (52.66~59.31%), EA (34.65~46.98%) 추출물 순으로 나타났다. Nitrite (NO2) 소거능은 70% methanol (34.57~39.33%), DW (32.53~38.47%), EA (27.59~32.62%) 순으로 관찰되었다. β-carotene 탈색 저해능은 DW (41.55~50.97%), 70% methanol (23.37~44.80%), EA (13.37~25.24%) 순으로 동정되었다. Reducing power (optical density)는 70% methanol (0.044~0.127), DW (0.033~0.099), EA (0.026~0.097) 순으로 확인되었다. 지질과산화 저해 능은 껍질 추출물 중 70% methanol (56.51~76.21%), EA (54.59~63.34%), DW (47.92~61.11%) 순 으로 관찰되었다(p<0.05). 이에, 치자 껍질은 기능성 식품 및 천연 항산화제로서의 가치가 매우 높을 것 으로 판단된다.

현재 식품공전에 식품원료로 사용이 가능한 것으로 등 록된 국내 산림지역 자생 식물을 식품산업에 활용하고자 국내 자생식물 45종을 선별하였고, 본 연구팀의 선행연구 에서 45종의 항산화 활성을 평가한 결과, 다른 종들과 비 교하여 우수한 항산화 활성을 보인 밤나무 잎을 본 연구 에서 사용하였다. 본 연구에서는 hydrogen peroxide로 산화적 스트레스를 유도한 상태에서 밤나무 잎 추출물의 세포 보호효과, 세포내 항산화 효과 및 항노화 효과를 관찰 하였다. 세포 보호효과를 측정한 결과 밤나무 잎 추출물 의 농도 유의적으로 세포를 보호하는 효과를 확인하였고, 특히 100 μg/mL에서는 양성대조군으로 사용한 항산화 물질인 ascorbic acid 수준까지 세포를 보호하는 효과를 나타내었다. H2-DCFDA 염색을 통한 세포내 항산화 효과를 확인한 결과, 형광현미경과 형광흡광도 측정에서 모두 밤나무 잎 추출물이 농도 유의적으로 세포내 ROS를 저감을 확인하였고, 세포 보호효과와 마찬가지로 농도 100 μg/mL 에서는 ascorbic acid와 비슷한 수준을 보였다. SA-β- galactosidase 염색을 통하여 관찰한 세포노화 억제 효과는 ROS 생성 억제 효과와 유사한 경향으로 밤나무 잎 추출 물의 농도 유의적으로 세포노화 억제를 관찰되었다. 이상 의 결과를 종합하여 볼 때 밤나무 잎 추출물은 산화적 스트레스로 인한 세포노화를 억제하는 효능을 보였으며, 이는 천연물에서 유래한 항산화 및 항노화 활성 첨가제로 활용도가 매우 넓을 것으로 판단된다.