잣나무림에서의 재선충병 발병양상을 분석하기 위하여 재선충병 피해지인 성남지역에 고정조사구를 선정(면적 1ha)하여 매월 주기적으로 감염목 발생상황을 조사하였다. 감염목의 시기별 고사율을 조사한 결과, 감염당해연도인 11월에 42.5%로 가장 높았으며, 7월에는 19.4%가 고사, 이듬해 5월에 2.4%로 가장 낮았다.
2013년 3월 중순, 재선충병 감염목의 고사시기에 따른 부위별 선충밀도 및 분포 상황을 분석한 결과, 감염 당해 연도인 7월부터 11월에 고사한 잣나무에서는 전부위에서 소나무재선충이 검출되었다. 그러나 이듬해인 1∼3월에 고사한 잣나무의 경우 수간하부에서는 거의 검출되지 않았으며 9m이상의 상부에서만 분포하였다.
고사시기별 매개충의 분포 양상을 조사한 결과, 9월 이전에 고사한 잣나무에서만 산란하였으며, 그 이후에 병징이 나타나는 감염목에는 산란하지 못한 것으로 조사되었다.
피해지 내 건전목의 하부고사가지에서의 매개충 산란 및 재선충 분포여부를 조사한 결과, 하부고사지 중 매개충이 산란 및 서식하는 가지는 전체 조사 가지 중 약 3.3%였다. 또한 매개충의 서식가지에서 소나무재선충을 분리한 결과 20%이상에서 소나무재선충이 검출되었다. 이 결과를 토대로 잣나무림에서의 소나무재선충병 방제 시 건전목의 하부 자연 고사지까지 완전히 제거하여야만 완벽한 방제효과를 얻을 수 있을 것으로 사료된다.
캄보디아는 동양구에 위치한 국가로 북동쪽으로 라오스, 동쪽과 남동쪽으로 베트남, 북쪽과 서쪽으로 태국과 접하고 있다. Samkos지역은 캄보디아 서쪽에 위치한 산림지역으로 1997년 유네스코 생물권 보호구역으로 지정된 바 있다. Samkos산은 1750m 높이로, 산림이 우거지며 야생동물이 다양하게 서식하는 지역이다. Samkos지역은 지속적인 화전과 산림훼손 등으로 인해 급격하게 환경이 파괴되고 있다. 본 연구는 2012년 7월 12일∼7월 23일, 2013년 2월 16일∼2월 18일까지 캄보디아 서부지역에 위치한 Samkos산에서 실시하였다. 연구방법은 Sweeping, Light trap, Bucket trap 등 다양한 방법을 이용하여 나비목의 다양성을 조사한 결과, 12년까지 7과 81속 98종이 채집되었으며, 13년도에 추가로 2과 50속 69종 채집되어, 현재까지 총 9과 131속 167종을 확인하였다.
Oak wilt disease caused by Raffaelea quercus-mongolicae was firstly reported at Sungnam city, Gyeonggi province, Korea, in 2004, and spread to several area of Korean peninsula. Raffaelea quercus-monglicae was transferred by ambrosia beetle, Platypus koryoensis, and caused oak wilt disease. Because oak wilt disease is mediated by ambrosia beetle, Platypus koryoensis, control of this disease concentrates on managing the ambrosia beetle population. Until now, several control methods such as felling and fumigation of dead oak trees with metham sodium, sticky-roll trap for catching emerged or attacking beetles, and oak tree logs for attraction of ambrosia beetle have been mainly used to control oak wilt disease in Korea. Although effective, current control methods have many side effects. Felling of oak tree is very difficult and dangerous to workers. To reduce the side effects of conventional control methods, new and safe types of control method for managing the ambrosia beetle populations are essential.
In this study, we investigated the number of caught beetles, effect of height and direction of collection bottles by using newly developed mass capturing device for P. koryoensis to evaluate the effectiveness of this device as practical use in field.
The intracellular endosymbiont bacterium Wolbachia is currently considered the most abundant in arthropods and has also been isolated from nematodes, amphipods, isopods, mites and spiders. Recently, Wolbachia-based research was focused on the control of disease vector-population, such as several mosquitoes such as genus Aedes and Anopheles which cause dengue fever and malaria, respectively. For the analysis of regional difference between vector mosquito Aedes albopictus and Wolbachia, we selected different regions and collected Ae. albopictus which were distinguished with mountain chain and waterway. Whole genomic DNA were extracted from collected specimens with 9 regions. PCR analysis and sequencing were accomplished in each specimen for Wolbachia detection and identification using WSP gene. As a results, almost mosquitoes were infected with two strain of Wolbachia both wAlbA and wAlbB. However, regional separation of vector mosquitoes, wAlbA strain of Wolbachia were showed more than 98% sequence similarity. In this study, we first reported that Wolbachia infection and type of Wolbachia in Korea and endosymbiont Wolbachia was showed highly sequence homologies.
The oviparous female and male of Stomaphis betulidahuricae Zhang and Qio, 1999 (Aphidoidea: Lachninae) are described and illustrated in detail for the for the first time. Notes on distribution, biology and host plants are presented. Also, the morphology of the male genitalia are illustrated.
Caloptilia Hübner, 1825 is one of the largest group in Gracillariidae. 314 species have been known around the world. To date, 15 species were known from Korea. In the present study, four species of Caloptilia are newly recognized from Korean insect fauna: C. fidella (Reutti), C. hidakensis Kumata, C. illicii Kumata, and C. pulverea Kumata. All of 19 Korean species, including four newly recognized species, are redescribed with illustrations of adults and genitalic structures.
We report Conilepia nigricosta (Leech, 1988) in Korea for the first time. The female of C. nigricosta is nearly identical to the female of Lithosia quadra, and it has caused confusion in the generic classification. Therefore, we reexamined their morphology and also performed to analyze DNA barcode region compared with species of other related genera. As the results, the significant diagnoses between the two species were reconfirmed and provided in the adult appearances, wing venations, and genital structures in both sexes. The DNA barcoding results also showed a large genetic distance ranging from 6.21% to 7.16% between the two species. In phylogenetic relationship for these two species as well as 28 related species of Lithosiina, C. nigricosta was clustered as a sister to L. quadra and they formed a strong monophyletic group. It supports a possibility that these two genera may be a single genus, but more detailed molecular analysis using additional genetic markers and samples is needed to resolve reliable phylogenetic relationships between the taxa examined in this study.
Nutritional conditions experienced during early growth have important implications for the lifetime fitness of herbivores. We investigated how the early life effects of imbalanced nutrient intake can be overcome in a generalist caterpillar, Spodoptera litura (Lepidoptera: Noctuidae). Over the fifth larval instar, caterpillars were pretreated on one of three diets that varied in protein: carbohydrate balance (p35:c7, p21:c21 or p7:c35). After molting to the sixth instar, they were transferred to one of three no-choice diets (p35:c7 ,p21:c21 or p7:c35) or a food choice where they received two nutritionally complementary diets (p35:c7 versus p7:c35). Approximately 80% of caterpillars that had been protein-deprived (p7:c35) during the fifth instar molted to the seventh instar. The threshold body mass for pupal metamorphosis was 144 mg at the start of the sixth instar. When given a choice, caterpillars pretreated on the low-protein diet (p7:c35) selected significantly more protein than those from other diets (p35:c7,p21:c21). Our results suggest that caterpillars are not only capable of switching their developmental program to reduce the deleterious effects of a nearly deficiency of protein, but also flexible at adjusting nutrient preference store dress specific nutritional imbalances experienced early in life.
Nitidulid beetles of the genus Aethina Erichson, 1843 are a well-known group as harmful to agricultural and apicultural industry, especially including A. tumida, an American small hive beetle, which is a beekeeping pest.
The world-widespread genus Aethina, within subfamily Nitidulinae and family Nitidulidae, is presented 5 subgenera and 28 species in Palaearctic region. Until now, 2 subgenera, Aethina s. str. and Aethina (Circopes), and 3 species, A. (A.) flavicollis, A. (A.) inconspicua and A. (C.) suturallis, have been recorded in Korea, and we added one more species, A. (A.) maculicollis as a newly recorded species. The larvae of A. (A.) maculicollis have been reported as pests of mulberry fruits in Japan.
In this study, we report the taxonomic key to the genus Aethina of Korea, and morphological description of A. (A.) maculicollis.
독도는 울릉도에서 216.8Km 떨어져 있는 우리나라 최동단에 위치한 섬으로, 2개의 큰 섬인 동도와 서도, 그리고 주변의 89개 부속도서로 구성되어 있는 총면적 187,554m2로 섬 일대는 천연보호구역(천연기념물 제336호)으로 지정되어 있다. 독도는 화산 폭발로 형성된 화산섬으로 대부분은 가파른 절벽으로 이루어져 있어 생물이 살기 어려운 환경을 하고 있다.
이러한 지형적 특성으로 상륙이 어려워 과거에는 독도 생물에 대한 조사가 많이 이루어지지 않았으나, 2000년대부터 많은 조사가 이루어 지고 있다. 곤충의 경우 Jolivet(1974)가 독도잎벌레를 신종보고한 것이 최초이며, 이후 자연보존협회, 해양수산부, 환경부, 대학, 연구소 등에서 조사를 실시하여 독도의 곤충을 보고하였다. 가장 최근에는 오 등(2012), 강 등(2013), 유 등(2013)이 보고하여 지금까지 140종의 곤충이 보고되었다.
본 연구는 국립수목원과 국립중앙과학관 등 생물다양성기관연합 주관으로 실시한 생물상 조사결과로, 2012년부터 2013년까지 총 5회의 독도 조사를 실시한 결과 딱정벌레목, 파리목, 나비목 등 총 7목 32과 51종의 곤충이 확인되었다. 그 중 나비목에 속하는 세미창날개뿔나방 (Labdia semicoccinea (Stainton)), 순무좀나방(Leuroperna sera (Meyrick)) 등 5종의 분포가 새롭게 확인되었기에 보고한다.
The effects of thinning on community diversity of wood-boring beetles were examined in Japanese Larch (Larix kaempferi) forest in In-je, South Korea in 2013. Three study sites were selected and thinning in the plots was conducted in 2010, spring 2012, and autumn 2012, respectively. Wood-boring beetles were collected every week using the 12-unit Lindgren multi-funnel traps with pheromone lure (Ipsdienol +50/-50 40mg, Ipsenol +50/-50 40mg) from mid-April to early November. Total 5 families (Cerambycidae, Curculionidae, Dryophthoridae, Scolytidae, Platypodidae), 62 species, and 26,638 individuals were collected. Diversity indices were 2.08, 2.02 and 0.76, and evenness indices were 0.55, 0.49 and 0.19 in 2010, spring 2012 and autumn 2012 thinning area, respectively. Among wood-boring beetles, the number of species of bark and ambrosia beetles were accounted for 44.4, 41.9 and 46.3% in 2010, spring 2012 and autumn 2012 thinning area, respectively. The number of individuals of bark and ambrosia beetles were accounted for 86.9, 82.2 and 98.4% in 2010, spring 2012 and autumn 2012 thinning area, respectively. Our results showed that the density of wood-boring beetles was the highest in the most recent thinning area, suggesting that thinning timing can influenced on the abundance of bark and ambrosia beetles. Among them, Ips subelongatus, I. acuminatus, and I. sexdentatus are species that mainly attack Japanese Larch. Ips subelongatus emerged faster than others and was the most dominant species.
Generally, Cerambycidae inhabites in tropical and subtropical zones, and they are large family among Coleoptera and distributed over 20,000 species worldwide. They typically characterized by extremely long antennae, which are often as long as or longer than the beetle's body. Order Cerambycidae are divided into 11 subfamilies in the world, among them, there are 304 species of the 159 genera in 7 subfamilies from Korea up to date. The genus Anoplophora is belonging to the tribe Agniini in the subfamily Lamiinae. Most species of Anoplophora are showed their beautiful colors on the elytra, pronotum, antennae and ventral parts etc. These colors superficially seems like mere patterns of the integument. Usually, colored pubescent patches of the elytra in Anoplophora are arranged in round spots, in others they are arranged in transverse bands. And also, the species of this genus have very long antennae (about 1.3-1.6 times the body length in males, 1.0-1.5 times the body length in females of most species) then others and mostly body size are large, some species is over 50mm. In this paper Anoplophora freyi is newly recorded in Korea. Consequently, a total of three species of the genus Anoplophora are recorded in Korea.
Epigeic spiders (Araneae) were surveyed in apple orchards under different farming practices (organic and conventional) in Muju-gun, Jeollabuk-do, Korea. The study was conducted from April to October in 2013 with 2 weeks interval using pitfall traps. Collected spiders were identified as 20 species of 6 families from 819 individuals in organic orchardⅠ, 19 species of 10 families from 759 individuals in orchardⅡ and 16 species of 8 families from 209 individuals in conventional orchard for from 1,787 captured spiders. Biodiversity was analyzed with species richness, abundance and Shannon diversity index (H΄). Species richness was statistically higher in organic orchard than in conventional orchard. Organic orchardⅡ comprised the greatest abundance followed by organic orchard and conventional. But species diversity was the highest in organic orchardⅡ, intermediate in conventional orchard and the lowest in orchardⅠ. Lycosidae was the most dominant family in all orchards regardless farming practices. However, Linyphiidae dominated in organic orchards only. The most dominant species were Erigone koshiensis (Linyphiidae) in organicⅠ, Pardosa laura (Lycosidae) in organicⅡ and Arctosa pungcheunensis (Lycosidae) in conventional orchard.
The aim of the study is to report Radopholus similis detected from Agathis dammara in Thailand as new host plant. Existing host of R. similis was known as coffee, pepper, sugarcane and banana etc. This nematode in this study was observed morphologic character using Carl Zeiss Axioimager M2 and Axiovision Rel. 4.8 program. The result shows that a and b of the female was distorted toward maximum value of original description of species, and b′, c and stylet length was distorted toward minimum value of original description of species. This nematode was morphologically distinguished from originally detected R. similis, but measured value was similar in range of original description of species. For more information, molecular assay was also confirmed the R. similis with 98% homology with the sequence of the internal transcribed spacer region (ITS) of ribosomal DNA. We provided PCR-amplified ITS nucleotide sequence.
성덕댐 건설 과정 중에 육상곤충의 군집 변동을 파악하기 위하여 성덕댐 건설공사 구간의 5개 지점에서 2010년부터 2013년까지 연 4회씩 총 16회에 걸쳐 육상 곤충상 조사를 실시하였다.
조사결과, 총 16목 111과 453종 8,360개체가 채집되었으며, 한국 고유종 25종, 국외반출승인대상종 31종이 출현하였다. 분류군별 종수 비율은 딱정벌레목(175종, 38.63%), 노린재목(101종, 22.30%), 매미목(41종, 9.05%)의 순으로 나타났으며, 개체수는 매미목(2,615개체, 31.28%), 딱정벌레목(2,393개체, 28.62%), 노린재목(1,881개체, 22.50%)의 순으로 높은 비율을 차지하였다. 군집분석 결과, 평균우점도(DI)는 0.439, 다양도(H')는 3.277, 균등도(E')는 0.758, 풍부도(R')는 4.958로 나타났다. 주요 우점종은 돼지풀잎벌레(Ophraella communa), 일본멸구(Stenocranus matsumurai), 애긴노린재(Nysius plebejus) 등으로 조사되었다.
지점별 군집분석 결과, St.3, St. 4지점에서 연차에 따라 우점도가 증가하고 균등도, 다양도, 풍부도가 감소하여 군집의 안정성이 떨어지는 경향을 보였는데, 이는 지속적인 공사로 인한 환경교란이 가장 큰 요인으로 분석된다.
The nutritional quality of host plant is critically important for insect herbivores to maximize their fitness, but it is relatively unexplored whether the ingestion of a specific host plant will have the same effects on insects under different thermal conditions. We have used a multi-factorial experimental design to investigate how the nutritional quality of host plant and temperature interact to affect life-history traits in a generalist caterpillar Hyphantria cunea (Lepidoptera: Arctiidae) feeding on five different host plants. Caterpillars raised on Platanus occidentalis, Sophora japonica and Prunus x yedoensis exhibited substantially higher survival, faster growth and heavier mass at pupation than those on Cornus kousa and Betula platyphylla. Caterpillars developed more quickly and attained a smaller final body mass at higher temperatures, but the way that these traits responded to temperature differed by host plant. Caterpillars on P.occidentalis displayed a monotonic decrease in development time with increasing temperature, but the development time of those on P. x yedoensis declined with temperature in a biphasic manner. Furthermore, the rate at which pupal mass increased with decreasing temperature was much greater for caterpillars on P.occidentalis than those on P. x yedoensis.
Starvation resistance is an important fitness trait that is controlled by both environmental and heritable factors. The main objective of this study is to explore the genotype-by-nutrient interactions for starvation resistance and its correlating physiological traits in Drosophila melanogaster. In this study, we conducted a split-family quantitative genetic experiment, in which female adults of Drosophila from 19 isofemale genetic lines were allowed to ingest one of two synthetic diets that differed in protein-to-carbohydrate ratio (P:C = 4:1 or 1:16 with the P+C concentration of 120 g L-1) before they were assayed for starvation time and lipid storage. In all genetic lines, Drosophila flies that had fed carbohydrate-rich diet (P:C=1:16) resisted starvation better and stored more lipids than did those that had fed protein-rich diet (4:1). Importantly, the extent to which both starvation resistance and lipid reserves were affected by dietary P:C ratio varied greatly among different genetic lines of Drosophila, as indicated by significant genotypeby-nutrient interactions for these two traits. When the patterns of the bivariate reaction norm for body lipid and starvation resistance were compared across the genotypes, we found strong evidence for genetic variations in the pattern of energy storage and usage associated with maintaining survival under starvation in Drosophila.
We report the complete mitochondrial genome (mitogenome) of Apodemia mormo, which belongs to the lepidopteran family Riodinidae. The 15,262-bp long complete genome is comprised of 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and one major non-coding A+T-rich region, with the arrangement typically found in majority of Lepidoptera. The genes of A. mormo are interleaved with a total of 168 bp, which are spread over 16 regions and overlap in a total of 58 bp at eight locations. All tRNAs of the A. mormo mitogenome formed typical cloverleaf structure, except for tRNASer(AGN), which formed the truncated dihydrouridine arm. COI gene started with CGA, instead of canonical ATN as seen in other Lepidoptera. The 349-bp long A+T-rich region harbored the conserved sequence blocks, such as ATAGA motif, poly-T stretch, the conserved ATTTA sequence, and microsatellite A/T repeat that are typically found in Lepidoptera, but absent for tRNA-like pseudogene.
We newly sequenced mitogenomes of five skippers belonging to Lepidoptera to obtain further insight into characteristics of butterfly mitogenomes and performed phylogenetic reconstruction using all available gene sequences (PCGs, rRNAs, and tRNAs) from 85 species in 19 families in eight superfamilies. The general genomic features found in the butterflies also were found in the five skippers: a high A/T composition (79.3% - 80.9%), dominant usage of TAA stop codon, similar skewness pattern in various levels, consistently long intergenic spacer sequence between tRNAGln - ND2 (64-87 bp), the ATACTAA motif betweent RNASer(UCN) and ND1, and characteristic features of the A+T-rich region (the motif ATAGA, varying length of poly-T stretch, and poly-A stretch). The start codon for COI was CGA in four skippers as typical, but Lobocla bifasciatus evidently possessed canonical ATG as start codon. Phylogenetic analyses mainly yielded the consensus superfamilial relationships ((((((Bombycoidea + Noctuoidea + Geometroidea) + Pyraloidea) + Papilionoidea) + Tortricoidea) + Yponomeutoidea) + Hepialoidea) with a high support for most nodes, confirming the validity of Macroheterocera and its sister relationship to Pyraloidea. Within Rhopalocera the familial relationships (Papilionidae + (Hesperiidae + (Pieridae + ((Lycaenidae + Riodinidae) + Nymphalidae))) were strongly supported, confirming invalidity of the superfamily Hesperioidea. On the other hand, superfamilial relationships among Noctuoidea, Geometroidea, and Bombycoidea and the familial relationships among Saturniidae, Sphingidae, and Bombycidae were dubious, requiring further representative taxon sampling.
The mulberry white caterpillar, Rondotia menciana, belongs to the lepidopteran family Bombycidae, in which the domestic silkworm, Bombyx mori is included. In this study, we describe the complete mitochondrial genome (mitogenome) sequences of the species in terms of general genomic features and characteristic features found in the A+T-rich region. The 15,364-bp long genome consisted of a typical set of genes (13 protein-coding genes, two rRNA genes and 22 tRNA genes) and one major non-coding A+T-rich region, with the typical arrangement found in Lepidoptera. Twelve of the 13 PCGs start with typical ATN codons, except for the COI, which begins with CGA. Twelve of the 13 PCGs have complete stop codon, except for COII, which ends up with a single T. The 360-bp long A+T-rich region harbored the conserved sequence blocks that are typically found in lepidopteran insects. Additionally, the A+T-rich region of R. menciana contained one tRNAMet-like structure, which has a proper anticodon and secondary structure.