간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
International Symposium on Climate Change and Insect Pest (2009년 10월) 185건
Poster Presentation
101.
2009.10
구독 인증기관·개인회원 무료
We investigated the distribution and habitat characterization of Korean Osmia spp. in apple orchard of 32 localities during 2005 to 2008. A total of 6,737 Osmia spp. were collected, consisting of six species, which was Osmia cornifrons, O. pedicornis, O. taurus, O. benefica, O. satoi and O. jacoti. The rate of bee nested was 11.7%. Among collected 32 localities, Choongju showed the most collecting rate among other localities, which was 22.4% and 17.5% for Yeongju and 14.9% for Geochang. In case of collected six Osmia spp, O. cornifrons was 60.2%, which was the dominant species and O. pedicornis was 20.4% and O. taurus was 10.8%, respectively. In case of sex ratio, O. cornifrons was 1 : 2.7 and 1 : 5.6 for O. pedicornis and 1 : 2.3 for O. taurus. Osmia spp. was more collected at habitat conditions within apple orchard with plentiful pollens place and abundant water.
102.
2009.10
구독 인증기관·개인회원 무료
The small brown planthopper (SBPH), Laodelphax striatelleus (Fallén), is a insect vector of Rice stripe virus (RSV) in temperate countries such as Korea, Japan, China, and Taiwan. As SBPH is able to overwinter successfully in these areas, RSV disease in subsequent rice fields has been believed to be endemic. In Korea, however, the RSV disease outbreaks have been observed mainly but not continuously at some western regions since 2001, caused a severe damage to the rice production. Although many efforts are underway to explain the outbreak phenomenon, the exact related factors are not known yet. In the meantime of the study on SBPH population dynamics in 2009, we catched unusually large numbers of SBPH adults by aerial net traps, maximally over 900, in early June at western coastal counties such as Taean, Seocheon, Buan, Sinan, and Jindo in Korea. Age distribution changes of SBPH in winter and post-winter seasons at some selected fields shows that the adults might be not related to overwintering population. The adults of overwintering population emerged from early April. Newly hatched nymphs of first generation were found from mid-May. In late May, just before the unusual catch of adults, the developmental stages of SBPH were mostly below 5th instars. This means that the big adult populations would be results of mass migration of SBPH abroad. We present also spacial distribution and host relationship of overwintering population as well as viruliferous rate changes of immigratory population.
103.
2009.10
구독 인증기관·개인회원 무료
광릉긴나무좀은 신갈나무에 참나무시들음병을 매개하는 것으로 알려져 있어 산림생태계 건강에 위험요인이 되고 있다. 본 연구는 수간 위치별 천공수를 기준으로 광릉긴나무좀 밀도를 추정하여 광릉긴나무좀 밀도가 신갈나무 피해도에 미치는 영향을 조사하기 위해 수행되었다. 신갈나무 군락지인 우암산(경기도 파주시)과 계방산(강원도 평창군) 피해지에서 신갈나무 300그루를 무작위로 선정한 후 개별목의 피해도, 흉고직경, 수간내 위치별 A5 148X210(mm)당 천공 밀도를 조사하였다. 피해도는 건전, 천공피해, 고사 3단계로 구분되었고 천공밀도는 내리막과 오르막 방향 수간 상부(지표에서 1m), 하부(지표면)에서 각각 조사되어졌다. 신갈나무 피해도가 증가할수록 각 위치별로 천공수도 증가하였고 광릉긴나무좀은 내리막 방향 수간하부를 선호하였다. 또한 고사목들의 평균 흉고직경이 건전목 흉고직경에 비해 커서 광릉긴나무좀이 상대적으로 노령목을 선호함을 보여주었다. 신갈나무의 피해도는 내리막 방향 수간 상, 하부의 천공수가 증가할수록 신갈나무 피해도가 증가하여 이 부분의 광릉긴나무좀 밀도가 신갈나무 피해를 설명하는 주요 요인으로 판별되었다.
104.
2009.10
구독 인증기관·개인회원 무료
An ambrosia beetle, Platypus koryoensis, is known as a major vector of Korean oak wilt disease (KOW) to Mongolian oak and it is considered as the one of the major threat to forest ecosystem health. This study was conducted to assess damage on Mongolian oak inflicted by the ambrosia beetle on the basis of entry hole density and location within the trunk. Two hundred forty Momgolian oak trees from Mt. Uam (Gyonggi-Do, Korea) and 60 from Mt. Gyebang (Gangwon-Do, Korea) were randomly selected. Initially, the damage degree on an individual tree and the diameter at breast height (DBH) were measured, and followed by the measurement of entry hole density per 148 X 210 mm2 on lower trunk (above the surface of the earth) and upper (100 cm above the surface) on each upslope and downslope. The damage degrees were divided into three classes: Ⅰ indicating healthy tree, Ⅱ tree damaged by the ambrosia beetles and Ⅲ dead tree by the KOW. As the damage class of the Mongolian oaks increased, the number of the entry holes increased regardless of locations of entry holes. The density of entry hole lower trunk was higher than that in upper trunk, showing that the ambrosia beetle preferred to attack the lower trunk on downslope. In addition, the results showed a tendency that the average DBH of the oak trees increased as the damage class increased from Ⅰ to Ⅲ. This suggests that the ambrosia beetle prefers to attack older trees. The entry hole densities on each lower and upper trunk on downslope were principal components to assess the damage degrees of the Mongolian oak.
105.
2009.10
구독 인증기관·개인회원 무료
As part of an on-going malaria surveillance effort conducted by the Korea Center for Disease Concectration & Prevention (KCDC), a total of anopheline mosquitoes was tested for the presence of Plasmodium vavax 18s protein. Mosquitoes were collected (using black light traps) from 2 quarantine stations (Kimpo branch office of Incheon Airport National quarantine station, Paju city and Sokcho branch office of Donghae National quarantine station, Goseong county), identified to species, and tested by PCR for the presence of P. vivax 18s protein. The quarantine stations is located near de-militarized zone in Republic of Korea . A total of 140 Anopheles bellene, 3 An. lesteri, 153 An. pullus, 238 An. kleini, and 1,523 An. sinensis was tested. Two Anopheles bellene, Seven An. kleini, and Nineteen An. sinensis were positive for P. vivax 18s protein. Anopheles sinensis is the predominent Anopheline mosquito encountered in the northern part of the ROK and is a known malaria vector. Other potential vectors include An. lesteri Baisas & Hu and An. yatsushiroensis Miyazaki. Anopheles bellene haven't been reported as vector of malaria until now. However, in this study, positive results were detected from An. bellene collected at Paju city and Goseong county, 27 May and 22 Jun, respectively. Anopheles bellene as potential vector of P. vivax might be further studied.
106.
2009.10
구독 인증기관·개인회원 무료
과수에 발생하는 주요 해충상을 파악하여 방제 기초자료로 이용하고자 국내 중부지역 사과, 배, 포도, 복숭아 재배지역을 중심으로 1999년부터 2008년까지 부정기적으로 해충발생을 조사하였으며, 수원에서는 사과 및 배 해충의 발생을 정기적으로 조사하여 다음과 같은 결과를 얻었다. 사과해충 중 전 조사지역에서 해마다 연속적으로 발생하고 있는 종류는 점박이응애, 조팝나무진딧물, 사과굴나방, 은무늬굴나방 등이었으며, 과실을 직접 가해하는 복숭아심심나방과 복숭아순나방의 발생은 낮았지만 꾸준히 발생하였다. 또한 은무늬굴나방은 해마다 발생이 증가하는 경향을 보였다. 배에서는 조팝나무진딧물, 꼬마배나무이, 점박이응애 등이 주로 발견되었으며, 과실을 직접 가해하여 경제적 피해가 큰 가루깍지벌레의 발생도 해마다 관찰되었다. 수원지역에서 배 해충의 연간 발생정도를 비교한 결과 점박이응애와 조팝나무진딧물은 사과와 유사한 발생양상을 보였다. 포도에서는 박쥐나방, 볼록총채벌레 등이 주로 발생하였으나 포도 재배시 큰 문제는 되지 않았다. 복숭아에서는 점박이응애, 복숭아혹진딧물, 복숭아유리나방 등이 해마다 발생되었으며 심식충류에 의한 과실피해는 높지 않았지만 꾸준하였다.
107.
2009.10
구독 인증기관·개인회원 무료
나도진주거저리(Platydema recticorne Lewis)는 한국과 일본에만 분포하는 균식성 종으로 일본에서 유충의 형태와 유충먹이로서 구멍장이버섯류(Polyporus spp.)라고만 밝혀져 있었다. 이에 현장에서 한국산 나도진주거저리 집단을 관찰하고 그의 일부를 실내 사육하여 생활사와 숙주선호성을 밝혔다. 야외관찰의 결과, 번식장소와 먹이로서 목질화 되고 수명이 긴 민주름버섯류를 선호하였다. 그 가운데서도 특정 종에 대한 숙주 특이성이 있는 것으로 관찰되었는데, 특히 활엽수림에 나는 버섯으로 도장버섯속(Daedaleopsis)에 속하는 도장버섯(Daedaleopsis congragosa)과 삼색도장버섯(Daedaleopsis tricolor)을 가장 선호하였다. 그 외에도 구름버섯속(Coriolus), 줄버섯(Bjerkandera adusta)과 아교버섯(Merulius tremellosus) 등을 차선으로 선호하였다. 선호도가 가장 높은 도장버섯속(Daedaleopsis)에서 2006년 월별로 나도진주거저리의 출현율을 조사한 결과, 5월이 가장 높았고, 버섯의 부패단계별로 구분해서 관찰해 보았을 때, 4단계인 도장버섯속에서 출현율이 가장 높게 나타났다. 또한 형질이 다른 버섯들을 대상으로 나도진주거저리의 숙주 선호실험을 실시한 결과, 나도진주거저리의 숙주선택에 영향을 주는 요인으로 자실체의 부패정도, 두께와 수명 정도인 것으로 나타났다. 목질화된 버섯 속에서 유충 생활을 하여 관찰이 어려움에도 불구하고 실시된 실내사육(25.5～26.1℃; 63. 5～64.5% RH)의 결과, 알에서부터 성충까지 약 53일이 소요되었고, 월동은 성충과 유충이 혼재된 상태로 숙주버섯의 자실체 속이나 숙주버섯이 붙어있는 나무껍질 밑에서 진행되었다.
108.
2009.10
구독 인증기관·개인회원 무료
국가장기생태모니터링 지역 중 삼척시 산불피해지역 2개 지점과 미피해지역 1개 지점에서 나비목 곤충상을 2006년 5월부터 2009년 6월까지 총 14회에 걸쳐 조사하였다. 조사지점은 2000년 4월 7일부터 4월 15일까지 발생하여 23,794ha를 전소시킨 동해안산불 피해지점에 해당된다. T1지점은 북위 37° 13′ 49.7″, 동경 129° 18′ 34.0″에 위치하며 최대 산불 피해지로 식생은 가장 단순하고 초본류가 우점하고 있다. T3지점 또한 산불 피해지로 북위 37° 14′ 13.9″, 동경 129° 18′ 16.2″에 속하며 T1지역에 비해 피해 정도는 적은 지역이다. 미피해지역은 북위 37° 19′ 40. 0″, 동경 129° 12′ 10.9″으로 식생이 다양하며, 작은 하천 및 10여가구의 민가를 접하고 있다. 나비류와 나비목 유충은 주간 정성채집을 통해 조사하였고, 나방류는 버켓트랩을 이용하여 일몰 후 3시간동안 정량채집하였다. 지점별 나비목 종수 변화를 보면, T1지점은 2006년부터 2009년까지 순서대로 181종, 123종, 148종, 65종(6월까지)으로 나타났으며, T3지점은 112종, 151종, 172종, 118종(6월까지)으로 변화되었다. 연도별 우점하는 과는 2006년 밤나방과 111종, 명나방과 78종, 2007년 밤나방과 74종, 명나방과 73종, 2008년 밤나방과 76종, 자나방과 61종으로 동일하게 나타났다. 미피해군, T1, T3지역의 나비목 군집특성에 관해 고찰하였다.
109.
2009.10
구독 인증기관·개인회원 무료
In organic agriculture, various cover-crops have been used to control weeds. In this study, we have investigated the effect of Vicia tetrasperma (L.) Schred. (Eolchigiwandu) which is native to on major insect pests of pepper. Redpepper seedlings at 8 leaves stage were transplanted in 20th May 2009 into experimental field located in the farm of the department of agricultural biology, Suwon, Korea. A cover-crop cultivation plot was compared with a control plot mulched by black plastic-film. Density of aphids and damaged fruits by oriental tobacco budworm, Helicoverpa assulta, were surveyed ten times from 21 days after transplanting (DAT) to 82 DAT. Fifty and forty pepper plants were sampled to count the density of aphids and damaged fruits by oriental tobacco budworm per a plant, respectively. In current study, three aphid species namely, cotton aphid, Aphis gossypii, green peach aphid, Myzus persicae, potato aphid, Macrosiphum euphorbiae were collected. Overally, the density of aphids on pepper in the control plot was higher than the cover-crop plot. Especially, aphid density increased up to 67.44±26.8 in early stage of control plot, whereas aphid was not found in cover-crop plot. The rate of fruit damage by oriental tobacco budworm was significantly higher in the cover-crop plot than the control plot in early stage of pepper, however damaged fruit rate of pepper in the control plot was significantly higher since the middle of July.
110.
2009.10
구독 인증기관·개인회원 무료
Since pine wilt disease was first reported in Busan in 1988, this disease has become a serious threat to pine forests in Korea. A general survey for Bursaphelenchus xylophilus group (B. xylophilus, B. mucronatus) nematodes were carried out to determine their geographic range, host tree species and insect vectors, Monochamus species. Based on the survey results, B. xylophilus were found over 57 cities or district and a similar nematode, B. mucronatus also has been isolated over 30 cities or district including Jeju and Pocheon. The Asian type of B. mucronatus was founded from Pinus thunbergii and P. densiflora, generally associated with M. alternatus as its insect vector. The European type of B. mucronatus which was limited distribution to the central areas, such as Yeongwol, Bonghwa, Yeongdeok, was also found on P. koraiensis and P. desiflora associated with M. saltuarius. Both Monochamus species transmitted both Bursaphelenchus species and co-existed in Muju and Yeongdeok predicted as their range margin. Their range margins shifted northwards compared to 2006, seemingly as a result of climate change. Recently, the European type of B. mucronatus has been identified in other several areas since it was first reported in Pocheon in 2008. Several reports indicate the potential pathogenicity of European type of B. mucronatus and other species, B. sexdentati and B. lenoni in Europe. Therefore, sustainable survey and the study for the newly invading Bursaphelenchus species as well as the member of B. xylophilus group should be performed to protect Korean forests.
111.
2009.10
구독 인증기관·개인회원 무료
A survey was conducted in the northern conifer forests of Korea from February to May in 2009 for the Monochamus species vectored Bursaphelenchus species. Dead pine trees infested with bark beetle larvaes were collected from Pinus densiflora and P. koraiensis located in the following target areas: Pocheon, Yeongi, Jincheon, Chuncheon, and Bonghwa. A cerambycid beetle, M. saltuarius was only found from sampled log piles in target areas. Bursaphelenchus species carried by M. saltuarius was only isolated in Pocheon, Gyeonggi province. The nematodes recovered from 17 (71%) of 24 beetles from P. koraiensis and 12 (75%) of 16 beetles from P. densiflora. The average number of nematode per adult was 1,974 and 11,823 from P. koraiensis and P. densiflora, respectively. The nematode was also recovered from the inner bark of both Pinus species infested with M. saltuarius larvae. Bursaphelenchus species identification was performed by morphological characters and molecular analysis with ITS-RFLP patterns and sequences of ITS and D2D3 region. Both morphological and molecular characters match well with the original description of the European type of B. mucronatus. This is the first report of M. saltuarius species as a vector of the European type of B. mucronatus on both P. densiflora and P. koraiensis in Korea.
112.
2009.10
구독 인증기관·개인회원 무료
Three acetylcholinesterase (ace) genes were identified from Bursaphelenchus xylophilus and named as Bxace1, Bxace2 and Bxace3. Open reading fragments of Bxace1, Bxace2 and Bxace3 were composed of 622, 625 and 588 amino acids, respectively. Sequencing comparison with Torpedo ace gene identified cholinebinding site, catalytic triad functional site, three internal disulfide bonds and aromatic residues for the catalytic gorge. Transcriptional profiling by quantitative real-time PCR revealed that Bxace3 is more actively transcribed than Bxace1 (2-3 times) and Bxace2 (9-18 times) in both propagative and dispersal stages. The three ace genes were functionally expressed using baculovirus system. Kinetic analysis using three choline substrates demonstrated that BxAce2 has higher maximum velocity than BxAce1 (ca. 2 times) and BxAce3 (ca. 100 times) whereas BxAce3 shows lower Michaelis constant than BxAce1 (12.8 times) and BxAce2 (13.6 times). In inhibition assay using five organophosphates (OPs) and three carbamates (CBs), all the three BxAces were highly inhibited by paraoxon, DDVP and chlorpyrifos-oxon but not inhibited well by fenamiphos and fosthiazate. Interestingly, inhibition assay revealed that BxAce3 is less sensitive to all insecticides tested than other two BxAces. The inhibition kinetic data obtained in this study should provide essential information for the development of OP- and CB-based nematicidal agents againt B. xylophilus.
113.
2009.10
구독 인증기관·개인회원 무료
Dae-Weon Lee, Jong Bok Seo, Jae Soon Kang, A-YoungKim, Won Tae Kim, Yeon Ho Je, Kook Jin Lim, Si Hyeock Lee, Young Ho Koh
There are increasing interests in developing methods specifically detecting pathogenic Bursaphelenchus xylophilus. In order to develop a detecting method for B .xylophilus, at first we generated monoclonal antibodies (MAbs) specific to B. xylophilus, discriminating from other pine tree resident nematodes. Among 2304 hybridoma fusions screened. We finally selected a MAb clone, 9F10 and used for further study. To identify the antigenic target of MAb-9F10, we employed several biochemical methods such as SDS-PAGE, 2 dimensional electrophoresis, anion exchange chromatography, and immunoprecipitation to separate and isolate an antigenic target. Proteins from above methods were analyzed via nano-LC-ESI-Q-IT-MS. Peptides of GaLECtin were always detected from several proteomic analyses, suggesting that GaLECtin is the antigenic target of MAb-9F10.
114.
2009.10
구독 인증기관·개인회원 무료
Pine wood nematode, Bursaphelenchus xylophilus, is a causative organism to induce pine wilt disease in many varieties of pine trees. Until 2006, Monochamus alternatus had been known as the only insect vector of pine wood nematode in Korea which targeted on Pinus densiflora (Japanese red pine) and P. thunbergii (Japanese black pine). However, pine wilt disease was also reported from Korean pine tree (Pinus koraiensis) in 2006 and we found another insect vector, M. saltuarius, was involved to transmit pine wood nematode. Both Monochamus species were confirmed to transfer pine wood nematode to their hosts but, there is no detail information about other transmitted nematode. Especially Bursaphelenchus mucronatus is common species transmitted by Monochamus species which is morphologically closed to B. xylophilus. Moreover B. mucronatus have two genotypes; one is East Asian type and the other is European type. Both genotypes of B. mucronatus were found in Korea but, the host and vector information related to the genotypes of B. mucronatus was not clear. Monochamus saltuarius was collected from three different geographical locations and nematodes were extracted and identified. For the identification of the juveniles, nematode DNA was extracted and ITS-RFLP analysis was done by PCR and gel electrophoresis. The selected enzymes were Hinf I, Alu I, Msp I, Hae III, Rsa I. Most of Bursaphelenchus species carried by M. saltuarius, which collected from pine wilt disease-free area, was determined as European type of B. mucronatus. We will compare the nematode species and genotypes carried by M. alternatus and M. saltuarius. In addition the rate of nematode carrying insect and the average number of nematode per single insect will be counted and compared.
115.
2009.10
구독 인증기관·개인회원 무료
국내에서 분리된 Bacillus thuringiensis subsp. aizawai CAB109균주는 나비목 해충에 활성을 보이는 것을 확인 하였다. 특히 우리나라에서 난방제 해충으로 알려진 담배거세미나방과 파밤나방에 동시에 독성을 보이는 것으로 나타났다. B.t. CAB109 균주의 활성정도를 비교하기 위해 혈청형이 aizawai이면서 생물농약으로 시판중인 T제품과 S제품을 조사한 결과, B.t. CAB109, T제품, S제품은 담배거세미나방 2령충에 대한 반수치사농도(LD50)가 각각 1.3x105cfu/ml, 2.3x106cfu/ml, 5.2x105cfu/ml으로 나타났고 파밤나방 2령충에 대한 반수치사농도는 1.8x104cfu/ml, 1.3x106cfu/ml, 1.5x106cfu/ml으로 나타나 두 종 해충 모두에서 B.t. CAB109 균주가 독성이 더 높은 것을 볼 수 있었다. B.t. CAB109가 이미 알려져 있는 aizawai와 비교해 새로운 유전자를 소유하는지 확인하기 위해 Plasmid DNA를 추출하여 전기영동 한 결과 B.t. subsp. aizawai HD-133과 다른 패턴을 보이는 것을 확인 할 수 있었고 Cry1~Cry5의 primer를 사용하여 PCR을 진행한 결과 B.t. subsp. aizawai CAB109균주는 Cry1Aa, 1Ab, 1C, 1D를 B.t. subsp. aizawai HD-133은 Cry1Aa, 1Ab를 가지고 있음을 확인 할 수 있었다. Plasmid DNA의 여러 밴드에서 어떤 부분이 해충에 어떠한 영향을 미치는지 확인하기 위해 B.t.가 생장하기 어려운 조건을 만들어 배양한 결과 이전과 다른 B.t. 내독소단백질을 가지고 있지 않은 strain을 만들 수 있었다. 이두 CAB109 strain의 플라스미드 DNA를 확인한 결과 밴드의 차이를 확인할 수 있었다. 그리고 Cry- strain은 모 stain이 가지고 있는 Cry gene을 가지고 있지 않음을 확인 할 수 있었고 생물 활성결과 독성을 보이지 않음을 확인하였다. 이후 다른 B.t. CAB109가 다른 Cry gene을 가지고 있는지에 대한 PCR을 더 진행할 예정이며 새로운 Cry gene여부에 대한 연구도 더 진행해나갈 계획이다.
116.
2009.10
구독 인증기관·개인회원 무료
밀양지역 시설잎들깨 포장에서 발생되는 주요 나방류 해충은 들깨잎말이명나방, 담배거세미나방 및 파밤나방 이었다. 들깨잎말이명나방은 4월 하순부터 11월 중순까지 발생하여 수량에 큰 피해를 주는 해충이다. 이들 나방류 해충을 친환경적으로 방제하기 위해 친환경 농자재로 고시된 Bt제 및 아자디락틴을 이용하여 생물검정 하였다. 처리후 7일차 담배거세미나방 및 파밤나방 유충에 대한 Bt aizawai의 살충효과는 85%였다. Bt의 들깨잎말이명나방 유충에 대한 살충효과는 100%였으나 아자디락틴의 파밤나방 유충에 대한 살충효과는 75.6%였고, 담배거세미나방 및 들깨잎말이명나방에 대한 살충효과는 55%였다. 가을작형 시설잎들깨에서 들깨잎말이명나방에 대한 Bt aizawai 2종(NT0423 WP, GB413 SC)의 방제효과는 처리후 10일차에 95%~96%였으며, 아자디락틴의 방제효과는 83.5%였다. 담배거세미나방 유충에 대한 Bt제 2종 및 아자디락틴의 처리후 10일차 방제효과는 77~80%였다. Bt제와 아자디락틴의 혼용에 의한 들깨잎말이명나방 및 담배거세미나방 유충에 대한 처리효과는 단제 사용구보다 효과가 5%~10% 높았다.
117.
2009.10
구독 인증기관·개인회원 무료
The TSSM is one of the most destructive pests on various orchard trees and garden plants. Abamectin resistance mechanisms in two resistant TSSM strains (PTF, ca. 155-fold; AbaR, ca. 3700-fold) were investigated with an emphasis on target site insensitivity mechanism. Detoxification enzyme assays revealed that metabolic factors contributed by EST and/or P450are involved in abamectin resistance. Synergistic assays showed that enhanced detoxification enzyme activity is not enough to account for the high level of abamectin resistance, further suggestive of the involvement of additional resistance mechanism, most likely target site insensitivity. As a putative target site for abamectin, we cloned the cDNA fragments of glutamate-gated chloride channel (Tuglucl) and determined its full length nucleotide sequences. Amino acid sequence comparison among the four strains showed that the Gly323Asp mutation located in the third transmembrane domainis only found in the AbaR strain, suggesting it is likely associated with the target site insensitivity conferring a high level of abamectin resistance.
118.
2009.10
구독 인증기관·개인회원 무료
Han-Goo Ji, Ji-Hyeon Park, Bong-Ki Son, Hwa-Jeong Yeom, Sang-Rae Moon, Changmann Yoon, Chul-Sa Kim, Gil-Hah Kim
Using thirty-one essential oils with different manufactured T-tube olfactometers, attraction and repellent efficacy were proved against ggot-mae-mi, Lycorma delicatula. Among tested essential oils, spearmint was selected to have attraction efficacy (94.1%; P<0.001). Spearmint was also showed attraction efficacy in 10 and 2.5 ul, but did not showed in 1 ul. GC analyzed chemical constituents of spearmint were tested with different concentations (10, 5, 2.5, 1 ul). Carvone were showed activity in 10 and 5 ul, but limonene did not showed the activity. When mixing two compounds were showed activity in 10, 5, 2.5 ul as 78.8, 73.5, and 69.7%, respectively. Lavender was selected to have repellent efficacy (86.7%; P<0.001). Lavender was also showed repellency in 10 and 2.5 ul, but did not showed in 1ul. GC analyzed chemical constituents of lavender were tested with different concentrations, results have the activity linalool and terpinen-4-ol in 10 and 5 ul, but linalyl acetate and caryophyllene oxide did not have the activity. Spearmint and lavender showing attraction and repellency, respectively, were keep in lure during 24 hr for penetration with different concentration(30, 20, 10 ul), and attached to the center of Fly catcher, fly sticky trap. The trap was set in the field that founded frequently the L. delicatula. The number of L. delicatula caught in the traps were counted after 5, 10, and 15 days. In attraction efficacy of 20 ul concentration wㄷre high attracted the insect(P<0.001). 30 ul concentration were also have the high attraction (P<0.01). We are now tested the control efficacy in grapevine orchard using these materials.
119.
2009.10
구독 인증기관·개인회원 무료
The acetylcholinesterase (AChE) inhibition of 12 plant-derived insecticidal compounds (anethol, anisaldehyde, 3-carene, 1,8-cineol, ethyl-cinnamate, linalool, magnolol, ethyl-p-methoxycinnamate, p-methoxycinamic acid, safrol, terpinen-4-ol, and α-terpineol) towards adult mosquitoes were examined by using Ellman method. Result were compared with those of dichlorvos, a potent AChE inhibitor. 3-Carene strongly inhibited AChE (IC50, 5.78×10-4 M), although the inhibition of the compound was lower than dichlorvos (IC50, 1.45×10-4 M). These results indicate that 3-carene acts as an AChE inhibitor, although an involvement of other insecticidal mechanism(s) might not be ruled out.
120.
2009.10
구독 인증기관·개인회원 무료
병원성곰팡이 Beauveria bassiana의 실내 목화진딧물 살충율은 1X108 conidia/ml 농도에서 5일째에 leaf disc는 89.5%, 유묘검정 71.2%이었다. 오이하우스에 B. bassiana 포자는 살포 후부터 생존수가 낮아지기 시작하였고 5일 간격 3회 살포에서 생존수가 가장 높았다. 포장에서 B. bassiana 농도별 처리에 의한 목화진딧물 사충율은 농도가 높을수록 증가하였으며 5일째, 1X109 conidia/ml에서 76.8%로 가장 높았다. 또한 살포 간격에서는 3일 간격 3회에서 가장 높은 사충율을 보여 8일째부터 70% 이상의 살충율을 유지하였으며, 5일 간격에서도 후기에 까지 살충력을 유지하였으나 살충율은 70% 이하로 낮았다.
식물추출물을 백강균(B. bassiana)에 처리하여 발아율을 조사한 결과 울금, 계피 처리에서 발아가 이루어지지 않아 이들 2종은 혼합처리조합으로 부적합하였다. 식물추출물 처리에 의한 목화진딧물에 대한 살충력은 은행, 울금, 당귀, 계피, 편백, 담배잎 추출물에서 50% 이상이었다. B. bassiana 1X108 conidia/ml 농도에 식물추출물 500배액을 혼합하여 살포한 결과 담배잎과 혼합하였을때 7일째에 70%의 살충효과를 보였으며, 담배잎 추출물 100배와 백강균을 혼합한 경우 1X108에서 5일째에 75.6%의 방제가를 나타내었으며 50배에서는 1일째부터 71.1%를 보였다.
