High-income mushroom crops require complex farming. Therefore, we conducted a test to identify the optimum temperature for the production of antler-shaped Ganoderma lucidum using the King Oyster mushroom cultivation facility. T-N showed 0.28% of oak sawdust and 2.2% of nutritional source. The pH of oak sawdust was 6.0, indicating weak acidity, and that of rice steel was 6.6, indicating neutrality in nutrition source. Study on the quality characteristics of mushrooms showed that the number of days at 25°C were 5~6 and those at 30°C were 3~5; the representative length at 25°C was 57.5 mm and that at 30°C was 92.2 mm; the biological weight at 25 °C was 43 g, which was greater than that at 30°C.

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.

The purpose of this study was to breed a variety of stable productivity and high storage characteristics of white Hypsizygus marmoreus, which has high demand at domestic and global market due to a low bitter taste and the preference for white species. Accordingly, ‘Baekmaru’ was bred by hybridizing brown and white cap color species and backcrossing with white species. The ‘Baekmaru’ variety was bred by crossbreeding the brown and white species and backcrossing the white species. Through repeated cultivation of ‘Baekmaru’, a variety with a low contamination rate was selected when culturing the spawn for stable cultivation. As a results of demonstration test, the yield of ‘Baekmaru’ was 14% higher than that of the commercial variety. In addition, among the fruiting body characteristics of ‘Baekmaru’, diameter and thickness of the pileus were 16.43±15.27mm and 6.46±0.58mm, which were slightly higher than the commercial variety, and the hardness was 2.69±0.89N for the pileus and 3.09±0.89N for the stipe. The shelf life showed less change in thickness and color of pileus compared to commercial variety. The hardness of pileus of 'Baekmaru' was maintained in the range of 3.5 to 4.0N in the 4oC and 4oC and 20oC mixed treatment until 30 days of storage, and was higher than that of the commercial variety. Therefore, it was suggested to be excellent in storability.

As the importance of public health increases with the spread of infectious diseases, functionality has become a factor affecting consumers’ purchase of mushrooms. Therefore, the bioactive components of button mushrooms (Agaricus bisporus), which are generally known to promote button mushroom consumption, were analyzed. White and brown button mushrooms were compared and white beech mushroom (Hypsizygus marmoreus) were used as a control. White button mushrooms had higher sugar and inorganic potassium concentrations than brown button mushrooms, whereas sodium, magnesium, and vitamin C concentrations were not significantly different between the different button mushrooms. Moreover, there was approximately twice as much ergosterol in white button mushrooms than brown button mushrooms. Brown button mushrooms had higher concentrations of β-glucan and oxalic acid than white button mushrooms, but there was no significant difference in total organic acid content between the two mushroom types. High concentrations of the essential amino acids, ergothioneine, isoleucine, and leucine and the non-essential amino acids, glycine and alanine, were observed. Concentrations of the vitamin B group and total polyphenols were also high.

기후변화 시나리오는 기후변화로 인한 미래 영향을 평가하여 피해를 선제적으로 최소화하기 위한 기후변화 대응 및 적응정책 수립을 위한 과학적인 근거로 활용 되어 왔다. IPCC 6차 평가보고서(AR6)에 사용된 SSPs(Shared Socioeconomic Pathways, 공통사회경제경로) 시나리오는 기존 RCP(Representative Concentration Pathways, 대표농도경로) 시나리오에 사용된 복사강제력 개념과 함께 미래의 완화 와 적응 노력에 따른 5개의 사회경제 시나리오를 추가로 고려하였다. 가나는 국가 발전용량의 54%를 수력발전에 의존하고 있어 기후변화에 따른 강수량의 감소로 전력 부족을 경험하고 있다. 또한 강우특성의 변화로 인해 주요 작물인 카사바, 옥수수, 코코아의 생산량이 감소할 것으로 예측된다. 한편, IPCC 6차 보고서의 기 준 시나리오로 채택된 SSPs 시나리오는 5차 보고서에서 채택된 RCPs 시나리오에 비해 대기 중 CO2 농도 전망을 비관적으로 평가하고 있다. Business as usual(BAU) 시나리오(RCP8.5, SSP5-8.5)에 따르면 2050년대 CO2 농도는 RCPs 시나 리오의 경우 541 ppm, SSPs 시나리오는 565 ppm으로 SSPs 시나리오가 RCPs 시나 리오에 비해 대기중 CO2 농도 증가 속도가 빠른 것으로 전망하고 있다. 따라서 본 연구에서는 기후변화 시나리오의 통계적 상세화 방법인 Simple Quantile Mapping(SQM)을 사용하여 Coupled Model Intercomparison Project phase 6(CMIP6) 18개 General Circulation Model(GCM)을 활용하여 가나지역의 미래기후 변동과 불 확실성을 평가하였다.

In assessing the seismic safety of nuclear power plants, it is essential to analyze the structures using the observed ground motion. In particular, spatial variation in which the characteristics of the ground motion record differ may occur if the location is different within the site and even if the same earthquake is experienced. This study analyzed the spatial variation characteristics of the ground motion observed at the structure and site using the earthquake records measured at the Hamaoka nuclear power plant. Even if they were located on the same floor within the same unit, there was a difference in response depending on the location. In addition, amplification was observed in Unit 5 compared to other units, which was due to the rock layer having a slower shear wave velocity than the surrounding bedrock. Significant differences were also found in the records of the structure’s foundation and the free-field surface. Based on these results, the necessity of considering spatial variation in the observed records was suggested.

This study was performed to develop a Kdiet-index based on the definitions and characteristics of traditional Korean food. The Korean Food Pattern Index (Kdiet-index) for diseases was applied using dietary data from the 2017 National Health and Nutrition Examination Survey (KNHANES) and the association between Kdiet-index and metabolic disease was confirmed. The study subjects were 1,971 Korean adults aged >40, and the components were classified according to 14 criteria used to establish the Kdiet-index. Main ingredients up to the top 3 were designated using dish names, ingredients, and contents using KNHANES dietary data. Kdiet-index was classified using scores of ≤3 points, 4-7 points, and ≥8 points and total Kdietindex were calculated by summing dietary scores. Correlations between Kdiet-index and obesity, hypertension, hyperlipidemia, and diabetes were analyzed. The odds ratio (OR) and 95% confidence interval (CI) for obesity were 0.531, 0.385~0.732 and for elevated cholesterol (≥240 mg/dL) indices were 0.471, 0.282~0.788, respectively, which showed a significant decreased in the risk for each disease for Kdiet-index of ≥8 points. This study confirms that metabolically related clinical results improved significantly as Kdiet-index increased and that higher Korean food pattern indices are associated with lower risks of metabolic disease

본 논문에서는 1차원 오일러 보 요소(Euler-Bernoulli Beam Element)를 이용한 회전익기 축계에 대한 중량 최적설계를 수행하였다. 회전 축계의 특성을 고려해 비틀림(Torsion)과 베어링과 같은 축지지 강성 및 플랜지(Flange) 질량을 모두 고려하였고, 동적 안전성 확 보를 위해 고유치 해석을 수행하여 임계속도(Critical Speed)와 기어박스로부터 오는 치 변형 가진을 회피할 수 있도록 하였다. 축의 길 이는 고정된 상태에서 두께와 반경을 조절하여 중량 최적화를 수행하였으며, 최적화 과정은 2단계로 나누어 진행하였다. 1단계에서 는 비틀림 강도를 제약조건으로 하여 중량을 최적화한 후 2단계에서는 축계 안정성 확보 기준(Headquarters, U.S. Army Material Command, 1974)에 따라 축의 비틀림 강도에 대한 제약조건을 만족시키며, 축의 1차 모드가 임계속도를 회피할 수 있도록 축 1차모드 와 임계속도의 차이가 최대가 되도록 최적화를 진행하였다. 주어진 1차원 보 요소를 이용하여 최적설계를 한 결과를 3차원 유한요소 모델과 실제 제작된 축게의 시험결과와 비교하여 제안된 방법을 검증하였다.

In general, a high tension on the anchor and chain is placed when a ship at anchor is subjected to heavy weather. Mariners have to pay attention to whether dragging anchor occurs to keep the safety of the ship at anchorage since it is difficult to maintain the stable motion of ship and it causes collisions with other ships nearby. In this paper, the ship motion against the external forces was shown to obtain the fundamental data about characteristic of holding power due to nature of seabed at anchor, so practical trials were carried out in rocky area and muddy area using a trial ship around coastal area of South Korea. In muddy seabed, holding power showed reasonable tension values depending on the distance from anchor position of continuing swing motions of a ship corresponding to wind force. Meanwhile in rocky seabed, tension values on the chain appeared very high occasionally regardless of the distance from the anchor position and seemed to exceed its holding power to be the breaking strain of the chain although weather was not in a severe condition. Therefore, some of the cables laid on the seabed were presumed to be caught in a crack on the rock. It is assumed that even a small amount of external force may cause the chain to break in a moment in rocky seabed. Additionally, wind and current forces had a somewhat contradictory effect on holding power of the ship between them.

본 논문은 성장기 청소년의 영양 공급과 국내 원유를 통한 백색시유 소비에 중요한 역할을 담당하고 있는 학교우유급식사업의 후생효과를 분석하였다. 이를 위해 균형대체모형 기반의 시뮬레이션 방법을 이용하여 코로나19가 발생하기 이전인 2019년을 기준으로 분석하였다. 분석결과, 학교우유급식사업으로 생산자잉여는 125.7~141.0 십억 원이 증가하나 122.4~137.3 십억 원의 소비자잉여가 감소하여 대부분 상쇄되는 것으로 나타났다. 그러나, 학교우유급식을 통한 급식시장의 소비자들인 학생들의 후생이 156.0~157.5 십억 원 증가하는 것으로 분석되어, 이를 종합하면 총 후생 증가분은 1,560~1,575억 원으로 계측되었다.

이 연구는 전 지구적 환경 변화에 대응하려는 인류 공동의 노력에 발맞추어 과학교육 측면에서 기후 변화를 설 명하는 효과적 개념 체계로서 극지 소양 교육의 필요성을 제안한다. 이를 위해 현재 고등학생들의 극지 소양 현황을 양적 검사와 질적 면담을 통해 조사하고 시사점을 논의했다. 연구에 참여한 대상은 2개 고등학교 2학년 학생 329명으 로 극지 소양 원리를 참조해 개발한 진위형 25문항으로 이루어진 검사에 참여했고, 이들 중 13명이 면담에 참여했다.연구 결과, 극지 소양 원리의 일부 영역에서 다소 미흡한 이해와 함께 개념적 공백이 나타났다. 극지의 지리적 특징에 대한 지식이 견고하지 못하고, 빙권의 구성 요소와 주요 특성을 거의 알지 못했다. 이러한 개념의 결손으로 극지 변화 와 전 지구적 기후 변화의 작동 기작을 잘 연결하지 못했다. 학교 교육과정에서 충족되지 못한 개념을 학교 밖 매체로 부터 비판 없이 수용하면서 기후 변화의 기작을 다소 단조롭거나 왜곡되게 인식했다. 한편, 극지와 기후 변화에 대한 모호한 이해와 함께 이를 보완하기 위한 인지 전략으로 언어 정보, 비유, 시각적 관찰 등을 활용했다. 이상의 연구 결 과를 바탕으로 학교 과학 교육과정에 기후 변화를 체계적, 종합적으로 이해할 수 있는 새로운 지식 체계로서 극지 소 양 교육을 도입할 것을 주장했다. 이밖에도 타교과의 극지 소양 관련 지식의 일관성 검토, 기후 변화를 다루는 학교 밖 매체 정보에 대한 비판적 기준 제공, 학생들의 오개념 점검 및 사고 전략 파악 등을 시사점으로 제시했다.

This study investigates the interfacial reaction between powder-metallurgy high-entropy alloys (HEAs) and cast aluminum. HEA pellets are produced by the spark plasma sintering of Al0.5CoCrCu0.5FeNi HEA powder. These sintered pellets are then placed in molten Al, and the phases formed at the interface between the HEA pellets and cast Al are analyzed. First, Kirkendall voids are observed due to the difference in the diffusion rates between the liquid Al and solid HEA phases. In addition, although Co, Fe, and Ni atoms, which have low mixing enthalpies with Al, diffuse toward Al, Cu atoms, which have a high mixing enthalpy with Al, tend to form Al–Cu intermetallic compounds. These results provide guidelines for designing Al matrix composites containing high-entropy phases.

The purpose of this study was to investigate the biological activity of fucoidan, a sulfur-containing polysaccharide, on cytotoxicity and apoptosis in the human HT-29 colorectal cancer cell line using cell viability, Flow cytometry, Western blot, and RT-PCR analyses. Fucoidan inhibited the proliferation of HT-29 cells by 39.6% at a concentration of 100 μg/mL for 72 h. The inhibition was dose-dependent and accompanied by apoptosis. Flow cytometric analysis showed that fucoidan increased early apoptosis and late apoptosis by 65.84% and 72.09% at concentrations of 25 and 100 μg/mL, respectively. Analysis of the mechanism of these events indicated that fucoidan-treated cells exhibited increases in the activation of caspase-3, caspase-8, and PARP in a dose-dependent manner. These results suggest that fucoidan may inhibit the growth of human colorectal cancer cells by various apoptosis-promoting effects, as well as by apoptosis itself.

Paddy fields are important habitats for maintaining the biodiversity of agricultural ecosystems. Recently, as the need for safe food and biodiversity conservation has increased, the rice cultivation methods have undergone changes. This study was conducted to investigate the fish fauna in Environmentally Friendly Paddy fields (EFP) and Conventional Paddy fields (CP), and compare the characteristics of Misgurnus anguillicaudatus and Aphyocypris chinensis populations in EFP and CP. A total of 2,703 fish were collected and classified into six families and 13 species. Our results showed that there was a difference in the proportion of fish populations in the two paddy fields, and the number of individuals in EFP was higher than in CP. The weight values of M. anguillicaudatus were higher in EFP than in CP, and the total length, body length, and weight values of A. chinensis were higher in EFP than in CP. Conditional factor (K) values also showed similar trends in EFP and CP. Our results indicate that the farming practices in paddy fields have a significant effect on the characteristics of M. anguillicaudatus and A. chinensis populations, and the results of this study can be used as basic data for securing biodiversity in future agricultural ecosystems.

Various eco-friendly seed disinfection technologies have been developed due to the increase in the global demand for organic food. In order to produce sustainable and eco-friendly agricultural products in Korea, seed disinfection technologies for the production of wholesome seedlings have been researched in diverse ways compared to how they were examined in advanced countries. Both domestically and internationally, the eco-friendly seed disinfection technologies to secure the horticultural crops have been treated by biological or physical methods, such as hot water treatment with ultrasonic disinfection technology, or applying organic agricultural materials like organic acids in plant extracts. However, from a practical perspective, various applied technologies can be implemented in farm fields to disinfect significant agricultural crops, such as lettuce, Chinese cabbage, radish, watermelon, cucumber, paprika, red pepper, ginger, ginseng, and sesame seeds.