The purpose of this study is to elucidate the impact of authenticity on experience value and subjective well-being among visitors who have participated in direct experiential activities in experiential fishing villages. The research method used literature research methods and empirical research methods using questionnaires, and this questionnaire was composed by determining three major variables and seven constituent factors for each variable through factor analysis and conducting prior research and preliminary surveys. The survey was conducted from February 5, 2023 to April 5, 2023 among experimental fishing villages with excellent ratings for scenery (environment), service, experience, accommodation, and food, and four villages that can experience tidal flats and manage customers online. The survey was conducted from February 5, 2023 to April 5, 2023, among experimental fishing villages with excellent ratings for scenery (environment), service, experience, accommodation, and food, and four villages that can experience tidal flats and manage customers online. The results of this study are as follows. First, the factors of authenticity in experiential fishing villages include three sub-factors: objective authenticity, constructive authenticity, and existential authenticity. The factors of experience value include two sub-factors: emotional values and functional values. Subjective well-being is derived from positive emotion and life satisfaction. Second, upon examining the importance of authenticity in experiential fishing villages, it was found that existential authenticity and objective authenticity, in that order, have a significant impact on emotional values. However, constructive authenticity did not have a significant impact on emotional values. Third, in terms of functional values, constructive authenticity, existential authenticity, and objective authenticity, in that order, had a significant impact. Fourth, experience value, in the order of emotional values and functional values, had a significant impact on positive emotion and life satisfaction of subjective well-being. Therefore, it was confirmed that the authenticity of experiential fishing villages is important as a strategy to enhance experience value and subjective well-being. Especially, considering that the majority of visitors to experiential fishing villages are family-centered (86.5%), applying marketing management strategies to develop programs that enhance existential authenticity and improve emotional values could elevate the subjective well-being of experiential visitors.

Cho, Sungwon. (2023). “Speech Adjustment in Adverb According to the Gender of the Recipient”. The Sociolinguistic Journal of Korea, 31(4), 279-305. This article aims to examine the use of adverbs by Korean men and women in conversations with each other, depending on the gender of the interlocutor. It has long been argued in sociolinguistics that language use of one gender to the other can clearly adjusted, even if they belong to the same social group. Gender of the receiver, in this sense, can be considered as a factor in context which fosters certain speech-adjusting behavior. If men and women use different languages, typical gender specific speech style may be present in the speaker recognition, and as a result, intentional or unintentional accommodation may occur. However, there has been no research on how the speech of one gender is accommodated by the other gender in Korean. The frequency difference between male and female speakers were significantly smaller in single-gender dyads compared to mixed gender dyads, which leads to conclusion that speakers mimic more of receiver’s speech style when conversating with opposite gender.

This study aimed to address the increasing demand for technologies preventing mushroom fly damage. By monitoring the annual occurrence patterns of pests over several years and accumulating data, we conducted an analysis to evaluate the efficacy of preventive measures applied before the surge in mushroom fly infestation, typically observed in May. For preemptive control, physical measures involved installing air curtains at cultivation facility entrances and placing sticky traps and insect traps around entry points to block external entry and reduce internal insect density. Additionally, we applied an organic agricultural material, Dalmatian chrysanthemum extract, weekly alongside chemical control measures. To assess the reduction in mushroom fly populations, yellow sticky traps (15×25 cm) were placed at three locations within the mushroom cultivation facility, and the occurrence patterns before and after implementing preventive measures were compared. Compared to conventional practices, the application of preventive techniques resulted in a significant reduction, with a 60% decrease from 15 levels of mushroom flies/m² to 6 levels of mushroom flies/m² in May and a 40% decrease from 10 levels of mushroom flies/m² to 6 levels of mushroom flies/m² in June. While achieving over 50% efficacy during the peak mushroom fly season with preventive measures, we identified complementary actions such as blocking external sources (gaps in cultivation facility doors) and maintaining cleanliness around cultivation facilities (proper disposal of spent substrate) for further improvement. Comprehensive analysis and safety studies, including correlation analysis with contaminants and pathogens, are recommended to ensure the widespread adoption of mushroom fly preventive techniques for safe and stable mushroom production in the agricultural sector.

이 논문은 다사다난하고 혼탁한 시대에 아름다운 이야기를 하고 싶어 시작하였다. 조선후기 탄생한 󰡔창선감의록󰡕은 마음의 근원에서 진실되게 용서하는 것을 보여주는 소설이다. 󰡔창선감의록󰡕에서 용서는 서로서로 화해할 마음을 가질 때 가능한 것임을 깨우쳐주었다. 후에 악인 화춘과 심씨는 진심으로 죄를 자백하고 화진과의 관계를 회복하였다. 화진은 악인들의 잘못을 스스로 바로잡고자 애를 쓰지 않았다. 그는 악인들이 질 책임은 하늘에 맡겼다. 그는 서로가 회복될 때까지 오직 그들의 악행을 견디고 참았다. 단, 화진 자신은 일희일비하지 않고 하루하루 인간애와 충효를 다하였다. 작가는 작품 말미에 ‘이것이 인간의 본성이다’라고 명시하였다. 따라서 이 작품은 우리의 본성을 살 때 참된 용서와 회복이 일어난다는 것을 가르쳐주었다.

This study was to identify the effects of education on Korean foods preferred by elementary students and their sustainability. A survey was conducted on 5th-grade elementary school students. Korean food preferences were classified by exploratory factor analysis as creative, healthy, or considerate, and the effects of education on Korean dietary life were classified as cognitive or emotional. Dietary life sustainability was evaluated separately. Results showed that creative and considerate preferences had significant impacts on cognitive and emotional education effects and that a healthy preference type significantly impacted the emotional effect of education. Analysis showed that creative and considerate food preference types significantly influenced dietary life sustainability and that cognitive and emotional education effects mediated these relationships. Sex was not found to have a significant moderating effect. The study shows that Korean dietary life sustainability is influenced by education on topics that promote the value and excellence of Korean food and suggests that experiential education combining practice and theory should be used to increase interest in Korean food among elementary students. Additional studies are required to determine Korean food preferences to facilitate the development of a dietary life education program that enables students to understand and maintain healthy dietary practices.

오대산국립공원의 고등균류 다양성 및 분포 특성을 시기별, 고도별, 임상별로 조사하여 비교하였다. 오대산국립공원 내 계방산, 소금강, 월정사지 구 일대에 임상별 6개씩 총 18개의 고정조사구에서 고등균류를 채집한 결과, 542점의 표본을 채집하였으며, 동정한 결과 81속, 256종으로 확인되었 고 최신분류체계로 분류한 결과 2문, 6강, 19목, 56과로 확인되었다. 부생성 균류가 62.9 %로 가장 높은 비율을 보였으며, 다음으로는 공생성 균류 36.2 % 기생성 균류 0.9 % 순으로 조사되었고, 우점종은 맑은애주름버섯(Mycena pura)과 굽은꽃애기버섯(Gymnopus dryophilus)으로 확인되었다. 해발고도에 따른 고등균류의 분포는 200∼677 m 조사구에서 109종, 700∼999 m에서 109종, 1000∼1400 m에서 118종이 조사되었 으며, 채집 시기별 고등균류의 분포는 9월에 132종으로 가장 많이 조사되었고, 6월 33종, 7월 75종, 8월 99종으로 조사되었다. 임상별로는 혼효림에서 124종, 활엽수림 121종, 침엽수림에서 가장 낮은 105종이 조사되었다. 고도별, 시기별, 임상별 모두 부생성 균류가 가장 높은 비율로 분포하였다. 종다양도는 9월에 2.00, 혼효림에서 2.04로 가장 높았고, 고도별로는 유사한 종다양도를 나타냈다(1.93∼1.99). 종 균등도는 고도별, 시기별, 임상별로 비슷한 수준을 나타냈다(0.94∼0.97). 공통 발생 종은 모든 시기에 2종, 전체 고도에서 7종, 침엽수, 활엽수, 혼효림에서 14종이 확인되었다.

The main factor of biodiversity decline in major biological populations around the world is invasion of alien species. To protect native species, it is necessary to manage alien species. Recently, to eradicate ecosystem disturbance caused by alien species in Korea, many efforts have been made to capture individuals using nets and purchase captured individuals. However, there is no standard for classifying species due to the form of nest site or external characteristics of eggs of freshwater turtles. Thus, Mauremys reevesii eggs might be discarded due to mistaking as eggs of alien turtles. Based on more data, this study aims to compare and analyze external differences among eggs of Trachemys scripta elegans, Pseudemys concinna, and M. reevesii and use them as reference materials in the process of eradicating alien turtles. This study measured characteristics of eggs of the three turtle species. As a result of comparison, all variables of external characteristics of alien turtles and M. reevesii eggs showed significant differences. The shape of egg was also different, with eggs of T. scripta elegans and P. concinna showing a bicone shape and those of M. reevesii showing an ellipsoid shape. In conclusion, based on results of previous studies and the present study, eggs of M. reevesii, T. scripta elegans, and P. concinna are different in shape and structure. Thus, it is possible to distinguish between M. reevesii and invasive alien turtle using their eggs.

돼지의 체세포 핵이식(Somatic cell nuclear transfer,SCNT)은 인간에게 약리적 효과가 있는 단백질, 이종 간 장기이식(xenotransplantation)에 사용되는 장기, 질병 연 구 목적의 모델 동물을 제공한다. 특히 형질전환 돼지를 활용한 심장 이식이 세계 최초로 성공한 후 형질전환 돼 지 생산의 안정화는 다음 연구를 위한 중요한 점으로 대 두되고 있으나, 미니돼지의 체세포 핵이식 배아의 생산 효율은 아직 낮은 실정이다. 형질전환의 성공은 양질의 SCNT 배아 생산에서 시작되어야 한다. 이러한 SCNT 배 아의 생산 효율을 향상할 수 있는 요인 중에는 공여 세포 의 형태가 있으며, 성공적인 공여 세포의 생산을 위해서 는 종축에 따른 세포의 특성을 파악하여야 하고, 혈액형 의 차이에서 발생하는 문제점 해결을 위해 OO 타입의 선 별이 필요하다. 본 연구에서는 지속적인 계대 배양을 통 하여 공여 세포로 사용되는 미니돼지의 태아섬유아세포의 계대 배양 조건을 확립하고자 한다. 또한 미니돼지의 혈 액형을 PCR 기반으로 분석하여 분류하고 OO 타입의 선 별을 통하여 이종 간 이식에 용이하게 공여 세포의 조건 을 확립하였다. 이후 sgRNA(single guide RNA)를 사용하 여 CRISPR-Cpf1로 GGTA1(α-1,3 galactosyl-transferase) 유전자를 knock-out 한 미니돼지의 생산으로, 급성면역반 응을 유발하는 Gal(1,3)Gal epitope이 제거된 미니돼지의 세포 주를 구축 및 체세포 핵이식을 통해 GGTA1 knock-out 미니돼지를 생산하였으며, 이러한 연구는 이후 체세포 핵이식 및 이종 간 장기이식에 중요한 기초자료로 사용될 것이라고 생각된다.

Microbial proteases are more economical than plant- and animal-derived proteases due to their ease of production and high activity. This study aimed to optimize the production of proteases from fermentative food-derived microorganisms. Five strains with proteolytic activity among 50 Bacillus sp. were first screened. Two strains with high protease activity were identified: Bacillus amyloliquefaciens SRCM 102139 and Bacillus subtilis SRCM 104999. SRCM 102139 strain and SRCM 104999 strain had the highest protease activity in 0.8% glucose and 0.3% yeast extract, and in 0.8% starch and 0.1% soy peptone, respectively. The production of protease for two strains was optimized by the Central Composite Design (CCD) under response surface methodology. The optimal conditions for protease production in SRCM 102139 were 0.5% and 0.347%, pH 6.0, for carbon (glucose) and nitrogen (yeast extract springer 0202) sources, respectively, with a predicted value of 0.929 U/mL. Additionally, the optimal conditions for protease production in SRCM 104999 were 0.5% and 0.5%, pH 6.7, for carbon (starch) and nitrogen (soy peptone HSP-349) sources, respectively, with a predicted value of 0.431 U/mL. The actual protease activities of SRCM 102139 and SRCM 104999 under the established conditions were 0.926 U/mL and 0.428 U/mL, respectively, closely matching the predicted values.

중수는 경수와 다른 물리화학적 특징으로 다양한 생물화학적 변화를 유도할 수 있다. 기존 분리공정의 단점인 에 너지소비량을 줄이고자 전기방사 폴리아마이드 분리막을 이용하여 정삼투공정을 이용하였다. 유도용액으로 NaCl과 인산을 사용하였다. 중수농도를 정량화하기 위해 FT-IR 분광법을 활용하였다. 인산과 수소/중수소의 특별한 상호작용력을 분광학적 으로 확인하였으며, 정삼투공정으로 농축이 가능할 수 있다는 것을 관찰하였다.

월출산국립공원 내에 위치한 남생이(Mauremys reevesii) 대체서식지에서 사육 중인 남생이 15개체(암컷 6, 수컷 9)를 대상으로 개체 간 구애와 공격 행동을 관찰하여 사회적 상호작용을 알아보고자 하였다. 이를 위하여 2020년 5월 18일부터 2020년 10월 19일까지 연구지역에 CCTV를 설치하여 매주 3일(24시간/일), 총 64일 동안 녹화하였고 시간 당 10분 간격으로 분석하였다. 남생이의 구애행동은 총 884회 관찰되었고 수컷은 체중이 가장 가벼운 암컷(3회)보 다 가장 무거운 암컷(357회)에게 더 많은 빈도의 구애행동을 하였다. 남생이의 공격행동은 총 259회 관찰되었으며 암컷-암컷 간 30회, 수컷-수컷은 81회, 암컷-수컷은 148회 관찰되었다. 이를 바탕으로 파악한 우열관계는 전반적으로 암컷이 수컷보다 더 높게 나타났으며, 몸 크기가 클수록 순위가 높게 나타났다. 본 연구는 국내 최초로 남생이의 구애행동 빈도를 통해 번식가능성이 높은 개체를 확인하고 공격 행동에 따른 우열관계를 파악하였다. 연구결과는 멸종위기에 처한 남생이의 관리 및 보전을 위한 행동학적 기초 자료로 활용될 수 있으며, 남생이의 사회구조를 이해하는 데 도움이 될 것이다.

This study was conducted to evaluate the filtration performance according to the feed temperature composed of NaCl and the operating pressure of the brackish water reverse osmosis (BWRO) process. The temperature is known that decides the filtration performance of reverse osmosis (RO). It is noted that temperature increase activates the permeate of salts due to augment of diffusivity and mass transfer. Filtration of the lab-scale RO system was performed with constant pressure and the constant flow was simulated. The salt rejection measured by the concentration of the feed and permeate was compared with water permeability and salt permeability in the conditions containing various temperatures (5, 10, 15, 20, 25, and 30℃) and pressures (10, 12, 15, and 18 bar). An increase in feed temperature from 5 °C to 30 °C caused a 4.65% decrease in salt rejection in CSM, due to an increase in salt permeability (4.06 times) rather than an increase in water permeability (2.62 times). Specific energy consumption (SEC) was calculated by using an electricity meter set in the RO system. It was expected that the SEC by the increases in temperature and pressure decreased due to the viscosity decline of the feed and the permeate flux augment, respectively. The SEC decreased by 63.4% in CSM and by 54.3% in Nittodenko when the feed temperature increased from 5 °C to 30 °C. It discussed how to operate the optimal RO process through the effect of temperature and operating pressure and the comparison of SEC.

In the automobile manufacturing industry, lightweight design is one of the essential challenges to be solved fundamentally. The vehicle wheels are classified as safety related components as the main substructure of the vehicle. In this study, we illustrate a technique for selecting the appropriate number of spokes. Based on the basic model of the selected number of spokes, we propose a method to maintain stiffness and design lightweight using topology optimization software. Based on the basic model of the selected number of spokes, it was redesigned to be lightweight while maintaining stiffness by utilizing topology optimization software. By comparing and reviewing the structural analysis results of the basic model and the redesigned model, a design technique that can maintain structural safety and reduce wheel mass was proposed.