본 연구는 우리나라 해안에서 널리 서식 중인 해양 자원 중 하나인 전복(Haliotis discus hannai) 의 차세대염기서열분석 데이터 기반으로 선별한 신규 펩타이드의 항암 활성을 평가한 연구이 다. 펩타이드의 항암 활성은 교모세포종 세포주인 SNU-489에서 농도 의존적으로 처리 시간에 비례하여 증가하였으며, 200 μM로 48시간 처리하였을 때 암 세포 사멸율이 67%로 가장 높게 나타났다. 반면 정상 세포인 HaCaT에서 가장 높은 세포 사멸율은 18%로 농도 의존적이었으나 처리 시간과는 무관하였다. 또한 신규 펩타이드의 항암 메커니즘 과정을 밝히기 위해 세포자 멸괴사(Necroptosis) 관련 유전자의 발현 변화를 qRT-PCR 방법을 통해 검증하였다. RIPK3는 신 규 펩타이드 처리군에서 200 μM 처리 시 9배 이상 발현 증가, MLKL는 100 μM 처리군에서 대조군 대비 2배 이상 유의미하게 발현이 증가되었다. 이러한 결과로 미루어 볼 때, 전복 유래 신규 펩타이드는 암 세포 특이적으로 세포 독성을 가지며, 세포자멸괴사 메커니즘을 통해 암 세포 사멸을 일으키는 것으로 추측되므로 신규 펩타이드가 추후 교모세포종 치료제의 후보 물질로 활용될 수 있을 것으로 사료된다.

The concern on the greenhouse gas emissions is increasing globally. Especially, the greenhouse gas emission from fisheries is an important issue from the Paris Climate Change Accord in 2015. Furthermore, the Korean government has a plan to reduce the GHG emissions as 4.8% compared to the BAU in fisheries until 2020. However, the investigation on the GHG emissions from Korean fisheries rarely carried out consistently. Therefore, the quantitative analysis of GHG emissions from Korean fishery industry is necessary as a first step to find a relevant way to reduce GHG emissions from fisheries. The purpose of this research is to investigate which degree of GHG emitted from the major offshore fisheries such as offshore gillnet fishery, offshore longline fishery, offshore jigging fishery and anchovy drag net fishery. Here, we calculated the GHG emissions from the fisheries using the Life Cycle Assessment method. The system boundary and input parameters for each process level are defined for the LCA analysis. The fuel use coefficients of the fisheries are also calculated according to the fuel type. The GHG emissions from sea activities by the fisheries will be dealt with. Furthermore, the GHG emissions for the unit weight of fishes are calculated with consideration to the different consuming areas as well. The results will be helpful to understand the circumstances of GHG emissions from Korean fisheries

The oocyte undergoes various events during In vitro maturation (IVM) and subsequence development. One of the events is production of reactive oxygen species (ROS) that is a normal process of cell metabolism. But imbalances between ROS production and antioxidant systems induce oxidative stress that negatively affect to mammalian reproductive process. In vitro environments, In vitro matured oocytes have many problems, such as excessive production of ROS and imperfect cytoplasmic maturation. Therefore, In vitro matured oocytes still have lower maturation rates and developmental competence than in vivo matured oocytes. In order to improve the IVM and In vitro culture (IVC) system, antioxidants, vitamins were added to the IVM, IVC medium. Antioxidant supplementation was effective in controlling the production of ROS and it continues to be explored as a potential strategy to overcome mammalian reproductive disorders. Based on these studies, we expect that the use of antioxidants in porcine oocytes could improved maturation and development rates.

The concern on the greenhouse gas emissions is increasing globally. Especially, the greenhouse gas emission from fisheries is an important issue due to Cancun Agreements Mexico in 1992 and the Kyoto protocol in 2005. Furthermore, the Korean government has a plan to reduce the GHG emissions as 5.2% compared to the BAU in fisheries until 2020. However, the investigation on the GHG emissions from Korean fisheries has not been executed much. Therefore, the quantitative analysis of GHG emissions from Korean fishery industry is needed as the first step to find a relevant way to reduce GHG emissions from fisheries. The purpose of this research is to investigate which degree of GHG emitted from the major coastal fisheries such as coastal gillnet fishery, coastal dual purpose fishery, coastal pots fishery and coastal small scale stow net fishery. Here, we calculated the GHG emission from the fisheries using the LCA (Life Cycle Assessment) method. The system boundary and input parameters for each process level are defined for LCA analysis. The fuel use coefficients of the fisheries are also calculated according to the fuel type. The GHG emissions from sea activities by the fisheries will be dealt with. Furthermore, the GHG emissions for the unit weight of fishes are also calculated with consideration to the different consuming areas. The results will be helpful to understand the circumstances of GHG emissions from Korean fisheries.

The aim of this study was to investigate change of plasminogen activators (PAs) and their inhibitors (PAIs) mRNA and protein expression level by heat stress in porcine endometrial cells. The endometrial epithelial cells were isolated from endometrial epithelium in porcine uterus and cultured in different temperature conditions (38.5 and 41.5℃) for 24 h. Expression of urokinase-type PA (uPA), tissue-type PA (tPA), PA inhibitor-1 (PAI-1) and -2 (PAI-2) mRNA in epithelial cells were analyzed using reverse transcription-PCR and protein levels were measured by immunofluorescence. In result, mRNA expression of uPA, tPA, PAI-1 and PAI-2 were decreased in 41.5℃ than 38.5℃ culture condition, however, significant differences were no detected. uPA, tPA and PAI-2 protein were mainly expressed in nucleus, whereas PAI-1 was distributed in cytoplasm and nucleus. uPA and tPA protein levels were increased by heat stress treatment and significant difference was only detected in tPA level (p<0.05). In contrast, two types of PAIs protein level were decreased in 41.5℃ cultured group compared with 38.5℃ group. In present study, tPA protein expression was upregulated by heat stress in porcine endometrial cells. This result suggest that change of tPA by heat stress may be related to blood flow into uterus and intrauterine microenvironments, and could directly and indirectly influence to reproductive performance in pigs.

Noncontact direct-printed conductive silver patterns with an enhanced electrical resistivity are fabricated using a silver ink with a mixture of silver nanoparticles and nanoplates. The microstructure and electrical resistivity of the silver pattern are systematically investigated as a function of the mixing ratio of the nanoparticles and nanoplates. The pattern, which is fabricated using a mixture with a mixing ratio of 3(nanoparticles):7(nanoplates) and sintered at 200oC shows a highly dense and well-sintered microstructure and has a resistivity of 7.60 μΩ·cm. This originates a mutual synergistic effect through a combination of the sinterability of the nanoparticles and the packing ability of the nanoplates. This is a conductive material that can be used to fabricate noncontact direct-printed conductive patterns with excellent electrical conductivity for various flexible electronics applications, including solar cells, displays, RFIDs, and sensors.

The aim of this study was to evaluate effect of α-linolenic acid (ALA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender containing ALA (0, 3, 5, and 10 ng/mL) with 0.05% ethanol. The frozen-boar spermatozoa were thawed at 37.5°C for 45 sec in water-bath. The spermatozoa samples were evaluated the plasma membrane integrity, acrosome reaction, and mitochondrial integrity using flow cytometry. In results, population of live sperm with intact plasma membrane was significantly higher in control and 3 ng/mL ALA treatment group than ethanol group (p<0.05). In contract, dying sperms were higher in ethanol group than 3 ng/mL ALA treatment (p<0.05). Acrosomal membrane damage in all sperm population was reduced in 3 ng/mL ALA groups compared with ethanol treatment (p<0.05). However, acrosome damage in live sperm population was no significant difference among the all treatment groups. Mitochondrial integrity was not influenced by ALA treatments in both of live and all sperm population. In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce the membrane damages including plasma and acrosomal membrane, whereas ALA did not influence to mitochondria in boar spermatozoa. Therefore, these results suggest that ALA can protect against the membrane damage derived cryo-stress, and cryopreservation efficiency of boar semen would be improved by use of ALA.

This study was to investigate effect of progesterone (P4) on prostaglandin (PG) synthases and plasminogen activators (PAs) system in bovine endometrium during estrous cycle. Endometrium tissues were collected from bovine uterus on follicular and luteal phase and were incubated with culture medium containing 0 (Control), 0.2, 2, 20 and 200 ng/ml P4 for 24 h. The PGF2α synthase (PGFS), PGE2 synthase (PGES), cyclooxygenase-2 (COX-2), urokinase PA (uPA), and PA inhibitors 1 (PAI-1) mRNA in bovine endometrium were analyzed using reverse transcription PCR and PA activity was measured using spectrophotometry. In results, COX-2 was higher at 2 ng/ml P4 group than control group in luteal phase (p<0.05), but, it did not change in follicular phase. Contrastively, PGES was significantly increased in 2 ng/ml P4 group compared to control group in follicular phase, but there were no significant differ among the treatments in luteal phase. uPA was no significant difference between P4 treatment groups and control group in both of different phase. PAI-1 was decreased in 20 ng/ml P4 group compared to control group in follicular phase (p<0.05). PA activity was decreased in 2 ng/ml P4 group compared to other groups in follicular and luteal phase (p<0.05). In conclusion, we suggest that P4 may influence to translation and post-translation process of PG production and PA activation in bovine endometrium.

The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed (37℃) thermos bottle by gloved-hand method and was separated by 65% Percoll with superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) before freezing. The frozen sperm was thawed at 38.5℃ for 45 sec in water-bath for sperm characteristic analysis. The sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction, Rhodamine123/PI double staining for mitochondrial integrity and were analyzed using flow cytometry. In results, sperm viability, acrosome reaction and mitochondrial integrity were improved in separated sperm groups compared with unseparated sperm by Percoll (UP) group. Especially, viability was significantly higher in sperm separated by Percoll containing 400 IU CAT group compared with other groups (P<0.05). And acrosome reaction was decreased in sperm separated by Percoll with 300 IU SOD, 400 IU CAT and 0.5 mM GSH groups compared with other groups, however, there were no significantly difference mitochondrial integrity among sperm separated by Percoll with antioxidant enzymes. In conclusion, we suggest that use of Percoll containing antioxidant enzymes for sperm separation will be beneficial for sperm cryopreservation in pigs.

Malaria is an infectious disease that cause chilling, fever and hepatosplenomegaly by protozoan in plasmodium invading red blood cell, carried by anopheles mosquitoes. Malaria is transmitting steadily in South Korea from 1993 when vivax malaria retransmission occurred to soldiers at the front line near North Korea. There are many attempts in academic fields to analyse the causes by carrying out correlation analysis considering weather elements and regional characteristics, also developing tools for analysis. However, existing analysis tools have many limitations relating multi-platform support, user associability, limitation of information and so on. This study is to improve these limitations by developing hybrid web app concepted malaria vulnerability map system. Thus, it is possible to check the real-time malaria risk information, even in mobile platforms, and maximize the support for ease of use by using the implements and game UI animation technology of mobile-specific functions. Further utilizing a frame animation technology is a game animation techniques visual representation, and visually provides a time zone of malaria risk information, it is attempted to increase the user access. The system is expected to utilized by experts in related fields and general users.

In order to achieve successful in vitro production of embryo, it is necessary to establish intrauterine environment during in vitro culture. Thus, this study was investigated to establish embryo culture system using co-incubated collagen matrix gel (CM) with endometrial epithelial cells (EC). Endometrial epithelial cells were isolated from porcine endometrium at follicular phase, the cells seeded in insert dish for co-incubation with CM-coated culture dish. Then, culture media treated with/without 2.0 IU/ml hCG or 10 ng/ml IL-1β. After incubation for 24 h, the co-incubated insert dishes were removed from CM-coated culture dish before embryo culture. Embryos at 48 h after in vitro fertilization (IVF) were cultured on the dish for 120 h with porcine zygote medium. We determined PTGS-2 expression in the ECs, VEGF protein in co-incubated CM with EC and observed cleavage rate and blastocyst development of embryos at 168 h after IVF. In result, expression of PTGS-2 was higher at co-incubated EC with hCG and IL-1β groups than EC without hCG and IL-1β. The VEGF protein was detected at co-incubated CM with EC, EC treated with hCG and IL-1β groups higher than CM group. Also, cleavage rate was no significantly difference among all group, however, blastocyst development was significantly higher in co-incubated CM with EC treated with hCG group than un-treated groups (p<0.05). Therefore, we suggest that novel embryo culture system using co-incubated collagen matrix gel with endometrial epithelial cells treated with IL-1β is beneficial and useful for enhancing the production of porcine blastocysts in vitro.

The negative factors of fishery in environmental aspect of view are Greenhouse gas emission problems by high usage of fossil fuel, destruction of underwater ecosystem by bottom trawls, reduction of resources by fishing and damage of ecosystem diversity. Especially, the Greenhouse gas emission from fisheries is an important issue due to Cancún meeting, Mexico in 1992 and Kyoto protocol in 2005. However, the investigation on the GHG emissions from Korean fisheries did not much carry out. Therefore, the quantitative analysis of GHG emissions from Korean fishery industry is needed as a first step to find a relevant way to reduce GHG emissions from fisheries. The purpose of this research is to investigate which degree of GHG emitted from fishery. Here, we calculated the GHG emission from Korean bottom pair trawl fishery using the LCA (Life Cycle Assessment) method. The system boundary and input parameters for each process level are defined for LCA analysis. The fuel use coefficient of the fishery is also calculated. The GHG emissions from the representative fishes caught by bottom pair trawl will be dealt with. Furthermore, the GHG emissions for the edible weight of fishes are calculated with consideration to the different consuming areas and slaughtering process also. The results will be helpful to understand the circumstances of GHG emissions from Korean fisheries.

This study is aimed to understand the vertical distribution of fish in the daytime and nighttime using an acoustic survey in Yondam reservoir of Jeollabuk-do, and an acoustical backscattering strength of dominant species, bass (Micropterus salmoides) and a bluegill (Lepomis macrochirus), which are classified as the ecosystem disturbing species. The results showed that the fish school was distributed in the shallow areas of less than 10 m depth during the period of Aug. and Oct. where it had a strong thermocline, otherwise, it was distributed over 10-times density under stable water temperatures as 13°C in Nov. There was no vertical patchiness difference between daytime and nighttime unlike the typical marine ecosystem. The dominant species were crucian carp, bluegill, bass. The hydroacoustic method can provide the spatial distribution and effective removal ways of the ecosystem disturbing species in inland fisheries.

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in 2.5x105cells/ml and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of IL-1β (0.1, 1, 10 and 100 ng/ml) were higher than without IL-1β, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p＜0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

현재 국내에서 채종용 양파의 수분을 위해 파리류가 사용되고 있으나, 개화적기 공급부족, 파리류 생산시 발생하는 악취 및 비위생성 등으로 많은 민원이 발생하고 있다. 이와같은 문제를 해결하기 위하여, 채종용 양파 소형망실(1.3m×2.3m×1.8m) 에서 기존에 사용 중인 파리류와 서양뒤영벌을 이용하여 화분매개효과를 조사한 결과, 서양뒤영벌이 70배 정도나 적었음에도 불구하고, 화분매개효과가 동등하거 나 다소 좋았다. 따라서 채종용 양파의 수분을 위해 뒤영벌이 파리류를 대체할 수 있을 것으로 판단된다. 소형망실에서 적정한 뒤영벌의 봉군은 일벌 50~80마리이 었다. 조생종 양파품종인 ‘샐러드-5’, ‘연신황’과 중만생품종인 ‘콜러서스’를 대상 으로 파리류와 뒤영벌의 화분매개효과를 조사한 결과, 서양뒤영벌이 파리류에 비 해 동등하거나 다소 좋은 결과를 보여주었다. 또한 화분매개효과에 있어서 뒤영벌 은 품종간의 차이가 없는 반면, 파리류는 품종 간에 차이를 보여주었다.

The endometrium undergoes a cyclic growth and tissue remodeling as changes of epithelial cells, and plasminogen activators (PAs) are related to endometrium tissue remodeling. This study was to evulate expression of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in porcine uterine epithelial cells. In results, the uPA and tPA were expressed in uterine tissue, epithelium and secretory glands in porcine endometrial cell. In addition, the uPA and tPA were expressed in cultured epithelial cells, and it were mainly expressed in cytoplasm. In porcine uterine tissue and epithelial cells, uPA activity was higher than activity in tPA. In PAs mRNA expression levels, uPA mRNA level was significantly higher than tPA mRNA level (P<0.05). The fluorescence intensity of uPA protein was also higher than fluorescence intensity of tPA protein, and uPA protein expression was significantly higher than in tPA protein expression (P<0.05). Therefore, we suggest that a physiological function in porcine uterine epithelial cells should be more influenced by uPA than in tPA during pre-ovulatory phase. The endometrium undergoes a cyclic growth and tissue remodeling as changes of epithelial cells, and plasminogen activators (PAs) are related to endometrium tissue remodeling. This study was to evulate expression of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in porcine uterine epithelial cells. In results, the uPA and tPA were expressed in uterine tissue, epithelium and secretory glands in porcine endometrial cell. In addition, the uPA and tPA were expressed in cultured epithelial cells, and it were mainly expressed in cytoplasm. In porcine uterine tissue and epithelial cells, uPA activity was higher than activity in tPA. In PAs mRNA expression levels, uPA mRNA level was significantly higher than tPA mRNA level (P<0.05). The fluorescence intensity of uPA protein was also higher than fluorescence intensity of tPA protein, and uPA protein expression was significantly higher than in tPA protein expression (P<0.05). Therefore, we suggest that a physiological function in porcine uterine epithelial cells should be more influenced by uPA than in tPA during pre-ovulatory phase.

최근 유전자원 유출문제, 해외채종지 인건비 상승 등으로 채종용 양파의 국내 채 종비율이 증가하고 있다. 채종용 양파의 수분을 위해 파리류를 사용하고 있으나, 개화시 적기공급, 하우스내 고온환경에서 수분률 저하, 악취 및 위생곤충 생산 등 의 문제가 발생하고 있다. 따라서, 파리류를 대체할 양파종자 생산에 적합한 수분 곤충을 선발하기 위해, 양파의 교배순화용상자(Ø=0.6m, h=0.8m)내에서 연두금파 리를 비롯한 서양뒤영벌, 머리뿔가위벌, 꿀벌 등 4종의 곤충에 대하여 화분매개효 과를 조사하였다. 화구수정률은 꿀벌과 서양뒤영벌이 연두금파리와 머리뿔가위 벌에 비해 10%이상 높은 결과를 보였다. 소화수정률은 연두금파리보다 꿀벌이 22%, 서양뒤영벌이 13% 더 높았고, 화구당 종자수는 연두금파리 대비 꿀벌 35%, 뒤영벌 30% 더 많았다. 이러한 결과로 볼 때, 교배순화를 위한 채종용 양파의 수분 을 위하여 꿀벌과 서양뒤영벌을 이용하는 것이 파리류를 대체할 수 있다고 판단된 다. 또한, 채종용 양파의 교배순화를 위해 꿀벌과 서양뒤영벌의 적정한 마리수는 꿀벌은 15마리 이상, 서양뒤영벌은 10~15마리였다.

본 연구는 농산부산물을 이용한 발효사료의 급여효과를 알아보기 위해 비육 흑염소의 생산성 및 영양소 이용률을 조사하였다. 증체조사를 위한 사양시험은 거세흑염소 24두를 이용하여 처리구당 6두씩 30일 수행하였고, 소화율시험은 거세흑염소 12두를 공시하여 처리구당3두씩 라틴방각법으로 실시하여, 처리구는 대조구(배합사료 및 볏짚 급여구)와 3개의 시험구(발효사료와 볏짚 급여구)로 각각 배치하였다. 일당증체량은 대조구가 가장 높았고, T3구가 가장 낮았다(p<0.05). 1일 두당 건물섭취량 과 유기물섭취량은 시판사료를 급여한 대조구가 각각 718.8과 680.9 g, 농산부산물 첨가구가각각 634.2~699.2와 602.8~660.4 g으로 나타나농산부산물 중 미강 첨가구가 높을수록 섭취량이 낮아지는 경향으로 나타났다. 건물과 유기물 소화율은 대조구가 농산부산물 첨가구보다유의하게 높았고(p<0.05). 시험구간에는 미강을30% 첨가한 T3구가 가장 낮았다(p<0.05). 질소축적률은 대조구와 T1구가 유의하게 높았다.따라서, 본 연구의 결과 농산부산물을 이용한발효사료(T1과 T2구)는 시판사료를 급여한 대조구와 비교 시 증체는 86~90%, 건물소화율은91.7~93.1%의 수준을 보였으며, 질소이용성은대등한 수준을 보였다. 그러나 미강의 첨가 비율이 높아질수록 사료효율 저하가 우려되므로조지방 함량을 5.0% 이하로 배합하는 것이 필요하다 판단된다.