In today’s world, intellectual property (IP) rights have become a significant part of overall corporate value as well as a “driver of important market transactions.” Nevertheless, patent commercialization and claims for damages due to patent infringement are often obstructed by parties’ differing positions on the patent value. To improve Vietnam’s legal and practical environment for patent valuation, this paper constructs a comparison of the prevailing frameworks of the United States and the People’s Republic of China with that of Vietnam to make recommendations meeting the stated purpose. An analysis of relevant academic literature suggests that parties should be allowed to choose their preferred valuation technique even when having engaged valuation professionals. Also, valuation service providers should be able to demonstrate the underlying rationale for selecting a particular valuation method. Other valuation techniques, such as forward citation counting, should also be studied to provide private parties, patent valuators and relevant state agencies in Vietnam with more options when facing the need to value a patent.

With the development of information technology, online food delivery services have significantly changed people’s food consumption patterns. The outbreak of COVID-19 has further facilitated the development due to restaurant restrictions and social distancing measures. Due to the emergence of online food delivery services, scholars have paid increasing attention to this popular topic, and a large number of studies have examined which factors are related to consumers’ intention to use online food delivery services. However, previous studies showed inconsistent findings, and relationships between variables lack a comprehensive understanding. Therefore, this study aimed to develop a research model and examine the factors influencing consumers’ intention to use online food delivery services. By reviewing and analyzing 52 studies with 53 independent samples (N = 22,937), the meta-analytic structural equation modeling approach was carried out to assess the research model. The findings indicate that two attributes of online food delivery services (i.e., convenience and price-saving orientation) play different roles in the research model. Specifically, convenience significantly impacted perceived ease of use but had no direct impact on perceived usefulness. Price-saving orientation significantly influenced both perceived ease of use and perceived usefulness. Moreover, relationships between perceived ease of use, perceived usefulness, and perceived trust were significant, and these factors ultimately significantly led to the intention to use food delivery services. The findings contribute to theoretical advancement in the extant literature on food delivery services and help firms develop to provide better food delivery services for consumers’ continuous usage.

While there is an increasing number of studies highlighting the power of videos in influencing audience attitudes and behavior, academic research in tourism is largely behind due to the methodological challenges of analyzing unstructured video data. This study adopts an automatic video analytics approach to examine the relationship between content features of pro-environmental videos and audience engagement in tourism. Artificial intelligence was used to extract video content features by detecting scenes and shots as well as labels (e.g., trees). Our findings suggest that there exists an inverted U-shape relationship between video informativeness and audience engagement. This study makes significant theoretical and methodological contributions to extant tourism literature by theoretically explaining and empirically testing how video content features influence audience engagement in pro-environmental video communications in tourism.

This study attempted to investigate how satellite fans’ nostalgia influences their attitude toward the European Professional Football Leagues (EPFL) sponsors and purchase intention toward EFL sponsors’ products. Based on the sample drawn from EPFL fans in Singapore, a conceptual model was developed. This study found that satellite fans’ nostalgia significantly affected attitude toward EPFL sponsors, which positively influenced their intention to purchase EPFL sponsors’ products. The findings of this study emphasize the critical role of sport nostalgia in affecting attitude and purchase intention and offer practical implications for marketers.

Seaports play a huge role in Vietnam's economy, being a border gate for economic and cultural exchanges with outsiders, especially the role of goods circulation. Container cargoes are one of the types of goods with large and increasing volume through Vietnam seaports. However, the heterogeneity between the seaport and the connected infrastructure greatly affects the capacity and efficiency of port investment. This is also one of the main causes leading to a shortage of goods, excess ports in some port areas. The root cause is that the planning has not kept up with the growth of the amount of goods arriving at the port, because the issue of forecasting the volume of goods through the port is not accurate. Therefore, it is necessary to develop models of forecasting container cargo through the ports with general, scientific, and high accuracy to serve the strategy, planning and development of seaport system; the work of planning and investment in the development of seaports, shipping fleets and other auxiliary transport infrastructure works. The purpose of this study is to build suitable forecasting models with high accuracy and reliability on the total volume of container cargo throughput of the Vietnamese seaport system. Based on the methods of a statistical survey, synthesis, regression analysis, and correlation to evaluate the influence of factors on container cargo volume through Vietnam's seaports in the period of 2004-2019. By incorporating more economic factors into the regression model, the paper focuses on forecasting container cargo through the Vietnamese seaport systems, going into cargo-based forecasting in tons and TEUs. The results of this study contribute to complete the rationale for forecasting, especially forecasts related to the shipping industry and the forecast for container cargo throughput of the seaport. Finally, selecting models for forecasting container cargo volume throughput of seaports by Vietnamese conditions.

This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response.

In this study, graphene oxide(GO) was used as drug carriers to amorphize poorly watersoluble drugs via a co-spray drying process. Two poorly water-soluble drugs, fenofibrate and ibuprofen, were investigated. It was found that the drug molecules could be in the graphene nanosheets in amorphous or nano crystalline forms and thus have a significantly enhanced dissolution rate compared with the counterpart crystalline form. In addition, the dissolution of the amorphous drug enwrapped with the graphene oxide was higher than that of the amorphous drug in activated carbon (AC) even though the AC possessed a larger specific surface area than that of the graphene oxide. The amorphous formulations also remained stable under accelerated storage conditions (40°C and 75% relative humidity) for a study period of 14 months. Therefore, graphene oxide could be a potential drug carrier and amorphization agent for poorly water-soluble drugs to enhance their bioavailability.

We investigated the effects of two Brucella proteins expressed in a pMAL expression system, RocF and EF-Ts, as subunit vaccines on immune modulation and protective efficacy using a mouse model. Mice vaccinated with MBP-RocF and MBP-EF-Ts displayed increased production of TNF, IFN-, MCP-1, IL-10 and IL-6, and TNF and MCP-1, respectively. Furthermore, mice vaccinated with MBP-EF-Ts showed decreased induction of IFN- and Th2-related cytokines, IL-10 and IL-6. Higher proportions of CD4+ and CD8+ T cells were observed in the blood of mice vaccinated with MBP-RocF than in the PBS-vaccinated group, although the increases were not significant. Furthermore, significantly reduced Brucella proliferation in the spleens of the MBP-RocF and MBP-EF-Ts groups were observed, but inflammation of these organs was not attenuated. Overall, these results indicate that RocF and EF-Ts could be potential subunit vaccine candidates against animal brucellosis.

Hydraulic and drainage properties play an important role in the serviceability of a permeable block pavement system. A serious impediment to its performance is the accumulation of sediments over time. The deposition of these particles in block pavement joints reduces infiltration rate and drainage capacity, which, in the long run, decreases the system’s life span. In this study, laboratory experiments were conducted in order to investigate the influence of particle deposition on the hydraulic conductivity of a type of block pavement system, permeable stone paver, without maintenance or cleaning. Coefficients of permeability before and after addition of fine particles were evaluated using a developed permeability equipment with constant head frame. Three gradations of silica sand were used to simulate road clogging particles. An equivalent of eight years of sediment loading was applied to three identical permeable pavement samples. Laboratory test results showed an average of 77% reduction on the permeability due to particle accumulation.

With interconnecting voids, porous asphalt provides drainage of rainwater in vertical and lateral direction during rainfall. In addtition, it also offers remarkable advantages compare to traditional asphalt: reduce vehicle splash and spray behind, reduce night time surface glare in wet season and increase tire-pavement friction...On the other hand, the following aspects are recognized as disavantages: reduced performance, winter maintenance issues and high construction cost. For flexible pavement, dynamic modulus master curve is an important parameter in the mechanistic-empirical pavement design guide. In this study, the results of experiment of dynamic modulus test of porous asphalt are discussed for understanding well about the viscoelastic characteristics of porous asphalt.

In Volvariella volvacea, a species of edible mushroom, cryogenic autolysis is a typical part of abnormal metabolism. Previous functional annotation cluster analyses of cold-induced gene expression profiles have shown that the ubiquitin-conjugating enzyme E2 (UBE2) and the cyclin-like F-box domain (FBDC) form the functional clusters. Among them, a specific FBDC gene has been found to be in response to cold stress and correlated with the cryogenic autolysis of V. volvacea. In this study, analysis of gene expression profiling showed that only one type of UBE2 in V. volvacea (UBEV2) was significantly up-regulated. Further quantitative real-time PCR analysis confirmed that the expression of UBEV2 was significantly up-regulated (P < 0.05) after cold-treatment lasting 4, 6, and 8 h. Western-bolt analyses confirmed that the protein expression of UBEV2 was in response to cold stress. This provided evidence that UBEV2 was a cold stress-responsive gene and closely correlated with cryogenic autolysis. The specific distribution of UBEV2 in recently diverged herb decay fungi indicated that UBEV2 was not evolutionarily correlated with early diverging fungi. Phylogenetic analysis indicated that UBEV2 was generated by horizontal gene transfer (HGT) from the ancestry of Selaginella moellendorffii UBE2. Further relative time estimation and detection of natural selection showed that there has been recent positive selection after HGT in UBEV2. Molecular modeling and logo analysis showed that the cysteine-cysteine motif is the characteristic of the UBEV2 family. These observations indicate that UBEV2 and FBDV1 are the newly discovered cold stress-responsive genes correlated with the cryogenic autolysis of V. volvacea.

This study evaluated the antimicrobial efficacy of different concentrations of ozonated water with organic matter, fetal bovine serum, at different concentrations and incubation times with bacteria. In the absence of organic matter, total eradication of up to 5 log of Escherichia (E.) coli was achieved, however, interference by organic matter led to inefficiency of ozonated water as a disinfecting agent. In addition, diminishing antimicrobial effects at higher temperatures, even in the absence of organic matter, were also demonstrated. These findings indicate that ozonated water will be a safe and effective disinfectant agent that could be useful in meat processing, especially an intestine processing, in Korean slaughter houses.

The objective of this study was to determine the efficacy of ozone in sanitizing water experimentally inoculated with the gram-positive food-poisoning bacterium Staphylococcus aureus. The bactericidal effect was measured after experimentally inoculated solutions were exposed to 0, 0.5, and 1.0 ppm ozone at several time points and different temperatures, in the presence of varying concentrations of different organic matter, namely, fetal bovine serum (FBS) or cattle liver. Results revealed inhibition of the bactericidal effect in the presence of the lowest percentage of FBS, but a lower extent of the inhibition occurred when liver was used as the organic matter. It was also apparent that a higher temperature and shorter ozone exposure time had led to a more reduced bactericidal efficacy than that under a lower temperature and longer ozone exposure. This study provides insight into the potential use of ozonated water as an effective and safe disinfectant in an abattoir setting.

Treatment of dextran sodium sulfate(DSS) on HeLa cells results to an enhanced susceptibility to Brucella(B.) abortus infection. An increase in the adherence, invasion and intracellular replication of B. abortus was observed in DSS-treated cells. Furthermore, a marked elevation in the intensity of F-actin fluorescence was also observed in DSS-treated cells compared with untreated B. abortus-infected cells. An upregulation of phagocytic signaling proteins by Western blot analysis demonstrated an apparent activation of ERK, p38α and JNK phosphorylation levels in B. abortus-infected DSS-treated cells compared with the control. Colocalization with LAMP-1 proteins was attenuated in DSS-treated cells upon intracellular trafficking of the pathogen compared with control cells. The results of this study demonstrated consistency with other pathogens. The uptake and intracellular replication of B. abortus is hypothesized to be stimulated by various dextran receptors such as C-type lectins that are involved in phagocytosis which can either be direct phagocytic receptors, modulators of the expression of other receptors or as opsonins leading to an enhanced internalization of B. abortus. The complexity of these interactions thus would warrant further investigation into the role of DSS in the pathogenesis of brucellosis. In summary, we conclude that DSS enhanced adhesion, phagocytosis and intracellular replication of B. abortus in epithelial cells which could lead to suppression of the innate immune system in chronic Brucella infection.

In Korea, a serious amphibian disease caused by the fungus Batrachochytrium dendrobatidis (Bd) has been reported from historical samples collected in the 1900s. In this study, we continue to evaluate the current prevalence of chytridiomycosis in the Korean Peninsula and we include imported frogs from America to our analysis. Non-invasive skin swabs were taken from 275 apparently healthy frogs, and Bd was detected in five free living frogs by the nested PCR protocol consisting of two species: Bombina orientalis and Rana catesbeiana, from Gyeongnam and Cheonbuk provinces. These frogs comprised about 2% of the total number of free living samples. This study might be useful for understanding amphibian chytridiomycosis in Korea.

Fast, cheap and sufficient serodiagnostic tools needs to be developed for the early detectionof brucellosis. Currently the tools cannot differentiate an active infection from vaccinated, norcan it differentiate other bacterial infections with lipopolysaccharides, especially Yersiniainfections. In this study, we purified recombinant outer membrane protein 10 and 28(rOmp10,rOmp28), and a dipstick assay(indirect or sandwich) was constructed with single(rOmp10 orrOmp28) and combined rOmps(rOmp10 and rOmp28) from Brucella(B.) abortus 544 to evaluatebovine Brucella positive serum collected during the beginning of the Korean outbreak from2006 to 2015. In application with single rOmp, rOmp10(70%; indirect, 92.11%; sandwichdipstick) and rOmp28(72.5%; indirect, 86.84%; sandwich dipstick) had comparable results. Inaddition, results indicated that dipstick with combined rOmps(rOmps10 and rOmp28) weresuperior in detecting positive serum samples, at 85% indirect and 100% sandwich dipstick. Surprisingly, the results were the same in detecting negative results at 97.78% for both singleand combined indirect dipsticks. The dipstick tools with rOmp10 and rOmp28 would be usefulfor a rapid screen method for bovine brucellosis.

The objective of this study is to examine the effect of food scandals on trust towards the corporate brand and purchase intention in Japan. Drawing on Mayer, Davis and Schoorman’s Model of Organizational Trust (1995) this study explores the effects of perceived trustworthiness, trust, and perceived risk on a consumer’s intention to purchase. In Japan, consumers were outraged when some top hotels and department stores were found to have mislabeled foods, selling cheaper alternatives instead of the expensive foods offered on the menu and using expired products (Grace, 2007; Japan Today, 2013; Kageyama, 2007; Onishi, 2007; Spitzer, 2013). The various food scandals have led to product shunning, fear, distrust and suspicion among consumers (Garretson & Burton, 2000; Niewczas, 2014; Smith & Riethmuller, 1999; Yeung & Morris, 2001). Thus this research was conducted to identify the influence of food scandals involving well-established Japanese supermarkets on consumer trust and risk perception. The influence of culture in shaping purchase intention was also explored. Trust itself is a concept that is elusive with myriad definitions ranging from Luhmann’s (1979) sociological theory of trust that looks as trust being a function of high perceived risk and experience to Deutsch (1973) who defines it as the willingness to be dependent on others in the belief that the other party will not disappoint intentionally. Luhmann (1979) argues that in order for trust to be apparent there has to be high-perceived risk. Various studies have also focused on trust being operational when there is risk‐taking behavior (Anderson & Narus, 1990; Canning & Hammer‐Llyod, 2007; Doney & Canon, 1997; Morgan & Hunt, 1994). In the food sector, as consumers lose control over knowing about the food we eat due to the increasing complexity of the food system trust becomes an essential component. Consumers have to trust food producers ranging from farmers to food companies as well as the public authorities to ensure food safety, quality and adequate supply. Consumers display different and inconsistent reactions regarding food safety that affects the perception of quality and their willingness‐to‐pay (Berg, 2004; Brewer & Rojas, 2008).