We investigate the changes of fatty acids in blood for an evaluation of the effects of soft and enteric coated capsules containing omega 3 fatty acids. Fish oil, which contained 62.87 g/100 g of sum of EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid), was used as nutracueticals for omega 3 fatty acids. Lipid releasing amount in soft capsule was 70% in stomach condition. However, there was 10% of releasing amount of lipid observed in enteric coated capsule in stomach condition. In intestinal condition, 50% of lipid releasing amount in enteric coated capsule showed until 6 hr, but soft capsule until 90 min. EPA and DHA contents in soft capsule administration showed higher level than those in enteric coated capsule until 8 hr. However, the administration of enteric coated capsule showed higher level of EPA and DHA in blood after 8 hr. After 24 hr, mono-, poly-unsaturated and saturated fatty acids contents with enteric coated capsule showed higher level than those with soft capsule. The enteric coated capsule containing omega 3 fatty acids was expected to sustain omega 3 fatty acids.

This study investigated the effects of the diet with Cucurbita moschata (CM), adlay seed (AS), and Cudrania tricuspidata leaf (CTL) mixed-powder on the visceral fat, fecal amount, and serum lipid profiles in rats fed a high-fat diet (HFD). The mixed ratio of CM, AS, and CTL as a functional food ingredient was 1:1:1 on a weight basis. Male Sprague-Dawley rats were fed a high-fat diet (D12492; 45 kcal fat) for 5 weeks during the first phase. In the second phase, which lasted 5 weeks, the rats were divided into six experimental groups. The groups were: a high-fat diet group as a control during the 10 weeks of experimental period; and a high-fat diet with 5, 10, 15, 20, and 30% mixed powder supplement group. The adipose tissue (RFP, EFP) weights were significantly decreased in the 20% and 30% mixed powder supplement groups than in the HFD group (p<0.05). The fecal weight produced by the 15%, 20%, and 30% mixed powder supplement groups was significantly increased during the second phase compared to the first phase (p<0.05). In terms of serum lipids, TG (triglyceride) content was decreased in the 10% and 15% mixed-powder supplement groups. The blood glucose levels were significantly decreased in all experimental diet supplement groups than in the HFD group (p<0.05). The findings show that the high supplement groups could improve the visceral fat accumulation, fecal amount, and blood glucose content compared to the HFD group.

Resveratrol (RVT) and epigallocatechin gallate (EGCG) individually inhibit adipogenesis in 3T3-L1 adipocytes. The objective was to examine the possibility of interaction between RVT and EGCG, resulting in enhanced inhibition of adipogenesis in 3T3-L1 adipocytes. Preadipocytes were treated with RVT and EGCG individually at 6.25 or 25μM (RVT6.25 or RVT25) and 12.5 or 50μM (EGCG12.5 or EGCG50) and in combination (RVT6.25 + EGCG12.5 and RVT25 + EGCG50). RVT25 as an individual compound decreased lipid accumulation in 3T3-L1 adipocytes by 24%, and RVT25 + EGCG50 further decreased lipid accumulation by 77%. In addition, exposure of 3T3-L1 adipocytes to RVT6.25 + EGCG12.5 and RVT25 + EGCG50 combinations resulted in an enhanced increase of adiponectin release and inhibition of leptin release. Quantitative analysis revealed that the combination of tested materials (RVT6.25 + EGCG12.5 and RVT25 + EGCG50) decreased the expression levels of C/EBPα, PPARγ2, and aP2. These results indicate that the combined treatments with RVT and EGCG produce synergistic effects on inhibiting adipogenesis in 3T3-L1 adipocytes. The overall results suggested that the combining RVT and EGCG might be more capable of exerting antiobesity effects than each individual compound by itself.

The purpose of this study was to investigate the effects of resistance exercise training on % body fat and insulin resistance in obese middle school male students. Subjects were composed of 20 students(exercise training group: 10, control group: 10, body

본 연구는 Amaryllidoideae속 수선과에 속하는 수선 품종 중 우리나라에서 중부지역의 화단용으로 지속적인 이용이 가능한 품종을 선발하기 위해서 실험을 실시하 였다. 피복의 유무에 따라 생육상태나 자구의 형성이 받 는 영향을 확인하기 위하여 수원소재 국립원예특작과학 원 노지 포장에 2010년 10월과 2011년 11월, 짚피복처 리구에는 생짚을 20cm 두께로 덮어주어 피복을 해주었 고, 무피복처리구에는 피복을 하지 않아 차이를 두었다. Tender III에 속하는 ‘tazetta. Shiro Busa’ 품종은 무피 복처리포장에서 모두 고사하였고 Hardy I에 속하는 ‘Cum Laude’, ‘Texas’ 품종과 Hardy II에 속하는 ‘Apotheas’ 등 14품종은 90-100%의 생존율을 나타내는 등 품종간 생존율에 차이를 보였다. 생존율에서는 차이를 보였지만, 생육상에 차이는 미비하였으며 화색과 부관의 색, 향기의 차이는 없었다. 그러나, Hardy I에 속하는 ‘Cum Laude’ 품종과 Hardy II에 속하는 ‘Apothease’, ‘Ice Follies’, ‘Orange Ice Follies’, ‘Replete’, ‘White Lion’는 초장, 부관의 폭, 화폭 등에서 평균간 유의성을 나타냈다. 자구의 수는 품종간 차이를 보이며, 연간 0-2 개 증가하였음을 확인하였다. 도입 수선품종에는 일반적 으로 사용되던 황색 수선 이외에도 백색, 오렌지색 의 화색을 지니고, 향기가 풍부한 수선도 있었다. 연구 의 결과, 도입수선 26품종 중 수원을 중심으로 한 중부 지방의 무피복상태에서도 화단용으로 지속적인 이용이 가능한 수선 품종은 RHS 분류체계 Hardy I에 속하는 ‘Cum Laude’ 및 ‘Texas’ 와 Hardy II에 속하는 ‘Apothease’, ‘Barrett Browning’, ‘Changing Colors’, ‘Dutch Master’, ‘Eastern Dawn’, ‘Garden Giant’, ‘Ice Follies’, ‘Obdam’, ‘Standard Value’으로 총 11품종이었다.

본 연구는 남해청 소속 5개 해양경찰서별(부산해양경찰서, 통영해양경찰서, 여수해양경찰서, 제주해양경찰서, 서귀포해양경찰서) 적정 인력배분에 대하여 구성원들의 인식을 조사하고 합리적인 해양경찰서별 인력배분 방안을 살펴보고자 하였다. 연구결과 첫째, 남해청 소속 구성원들은 조직진단의 필요성에 대하여 56.7%가 찬성하는 것으로 응답하였다.

본 연구는 가축 체지방 감소를 위한 수동면역학적 기법의 기초자료 확보를 위해 흰쥐 지방부위별(복강 및 피하지방) 특이 다클론 항체를 개발하고자 실시되었다. 흰쥐 부위별 지방 조직에서 지방세포를 분리하고 각각 primary 배양시킨 후 개발된 복강지방(AAb) 및 피하지방 특이 다클론 항체(SAb)를 주입한 뒤 media 내 lactate dehydrogenase(LDH) 방출 수준을 조사하였다. 희석배율 1:1,000배를 기준으로 비면역혈청은 항원-항체 결합 반응이 거의 측정되지 않았고, AAb 및 SAb는 희석배율 1:128,000배까지 각각 항원-항체 반응이 감지되었다. 부위별 지방과 비교했을 때, AAb 및 SAb는 흰쥐 타 장기들과는 특이한 반응을 나타내지 않았다(p<0.001). 본 연구에서 개발한 두 항체들은 모두 항원으로 이용된 부위의 지방세포 PMP와 가장 높은 반응을 나타내었으며, 지방세포 배양을 통한 LDH 수준 검사에서는 AAb 및 SAb 모두 비면역혈청에 비해 유의적으로 높은 세포파괴가 일어났음을 확인할 수 있었다(p<0.01). 이상의 결과를 종합할 때 본 연구에서 개발된 AAb 및 SAb는 높은 역가, 타장기 안전성 및 in vitro 지방 감소 효과가 있었다고 생각된다.

The bovine fatty acid binding protein 4 and 5 (FABP4 and 5) is a major positional and physiological candidate gene for the bovine marbling and carcass weight. The aim of this study was to evaluate the association between economic traits of Korean cattle (Hanwoo) and genetic variation in fatty acid binding protein 4 and 5 (FABP4 and 5) genes within carcass/meat quality traits and the before/after of fatting in breed Hanwoo. Here, we characterized the nucleotide polymorphism of FABP4 and 5 in 86 cattle. We were detected the variability of three types (GG, AG, and AA) by PCR, and economic traits were analyzed by the mixed regression model implemented in the ASReml program. As the result of statistical and supersonic analysis, FABP4 gene was highly showed significant effect (p<0.006) on marbling score (MS), in contrast FABP5 gene was lowed (p<0.084) on MS before fatting. But, FABP4 gene was highly showed significant effect (p<0.0054) on MS, in contrast FABP5 gene lowest (p<0.0899) on MS in the after of fatting. Compare to supersonic result before fatting in FABP4 gene, it was detected type GG: (p<7.18), AG: (p<8.50), and AA: (p<10.50) (n=50), showed type GG: (p<4.88), AG: (p<2.33), and AA: (p<0.00) after weed out (n=20). Futhermore, it was detected type GG: (p<9.30), AG: (p<7.95), and AA: (p<7.40) (n=50) before fatting in the FABP5 gene. It was shown type GG: (p<2.67), AG: (p<3.50), and AA: (p<5.00) after weed out (n=50). Our results indicate that FABP4 and 5 gene transcription is regulated by the environment of feeding and management, and suggest that feeding and management could be potential key in determining FABP4 and 5 genes transcription for carcass/meat quality traits in breed Hanwoo.

In previous studies, we performed joint animal studies and clinical trials between Yonsei University and Oryza Oil & Fat Chemical Co. Ltd. We have shown that coffee bean extract has potent anti-obesity and hypotriglyceridemic activities as well as beneficial effects on body fat reduction.In this study, the effects of coffee bean extract (100 mg/capsule) on body fat reduction were evaluated in overweight/obese women (body mass index of 25~30 kg/m2 or body fat 〉 30%) not diagnosed with any type of disease. Subjects were randomly assigned to a coffee bean extract group (n=10) or placebo group (n=10). We measured anthropometric parameters, abdominal fat distribution by computed tomography and blood components before and after the 8week intervention period. After supplementation, the coffee bean extract group showed body weight (p=0.08), body mass index (p=0.06), hip circumference (p〈0.05), and upper waist circumference (p〈 0.01). In addition, after 8 weeks, the coffee bean extract group showed a significant decrease in abdominal internal fat area compared to 0 weeks (0 weeks : 155.8cm2; 8 weeks : 145.9cm2, δ change : -9.9cm2, respectively). However, there were no significant differences in lipid profiles or serological measurements between the coffee bean extract group and placebo group. The results of our human study demonstrated that coffee bean extract supplementation for 8 weeks has beneficial effects on reducing abdominal internal fat area as well as hip and waist circumferences.

This study mainly inquired characteristics and changes of 'Chang-aelgool' through 38 cases(with 161 Ssang-chang) of annex and pavillion buildings in Yeong-nam region which are built during the Chosun dynasty. The method of inquiry included actual survey of windows along with bibliographical research, and the results are as below. First, through the discovery of the term 'Chang-aelgool' as an indication of the window-forming frame in 'YeongGeonUiGwe'(1680 A.D), it is apparent that the term 'Chang-aelgool' was widely used in Korea from the late 17th century. Second, the 'Chang-aelgool' of study objects are classified into 4 categories. Type Ⅰ and Ⅱ are comprised of mitre-joints which cover the 4 corners of 'Chang-aelgool' and mainly used in building annex and pavillion buildings during the early period of the Chosun dynasty. Type Ⅲ was widely used during the early and middle period of the Chosun dynasty and drastically dropped in number during the late period of the dynasty. Type Ⅳ is comprised of mitre-joint of the upper-half, tenon-jointing of the lower-half and widely used in annex and pavillion building during the late period of the Chosun dynasty. Third, the form of 'Chang-aelgool' has changed from rectangular form with longer width during the early period of Chosun dynasty to square form during the middle period and eventually ended up as a rectangular form with longer height during the late period of the dynasty. Fourth, it is considered that while mullion which is located in the center of 'Chang-aelgool' was mainly used around the main floored room during the early period of the Chosun dynasty, became commonly used in main floored room and 'ondol' rooms during the middle period and drastically dropped in number from then and ended up being not in use after the mid 18th century.

This study was carried out to investigate the effect of wormwood meal addition on constituent of blood, and liver fatty acid in broiler chickens. Two-day old 25 male commercial broiler chicks were distributed to 5 treatments (wormwood meal levels ; T1 (1%), T2 (3%), T3 (5%), T4 (10%), and control (C)) for 5 weeks. Free fatty acids of liver were a little different among the treatments and the palmitic, stearic, oleic, and linoleic acids were occupied 92.5% of total free fatty acids. Hemoglobin and hematocrite concentration of blood were significantly decreased (p<0.05) as the levels of wormwood meal increased, and serum albumin concentration of broilers was little different between 0 week and 5 week experiments. Also serum cholesterol, phospholipid, total cholesterol, and triglyceride concentration of liver in broilers were little different during the 5 weeks experiment. Serum triglyceride concentration was significantly increased (p<0.05) as levels of wormwood meal increased. These results indicate that dietary wormwood meal addition has a little positive effect on increasing unsaturated fatty acid and decreasing saturated fatty acid.

최근 자전거이용에 대한 관심이 증가하면서, 전국적으로 자전거도로 확충을 계획하고 자전거 이용 활성화를 위해 많은 노력이 이루어지고 있으나 대부분의 계획들은 도시부와 레저형 자전거도로에 치우쳐 있다. 따라서 본 연구에서는 지방부의 생활형 자전거도로 사업의 효율적인 투자를 위한 우선순위를 결정할 수 있는 평가기법을 개발하고자 한다. 먼저 평가지표 개발을 위해 지방부 생활형 자전거도로의 특성을 반영하고 정량화가 가능하여 객관적으로 평가할 수 있는 지표를 선정하였으며, 평가지표의 가중치를 산출하기 위하여 계층화분석법을 사용하였다. 또한, 평가항목의 결과만을 기준으로 사업의 우선순위를 평가할 경우 대도시 중심으로 우선순위가 분포되는 경우가 발생할 것으로 판단되어 지역적 형평성(지역균형발전효과)을 고려하기 위해 지역낙후도지수를 고려하였다. 지방부 생활형 자전거도로의 우선순위를 선정하기 위해 시범 적용된 일반국도 구간은 총 23개 구간 160km의 연장으로 구성되어 있다. 본 연구에서 제시된 지방부 생활형 자전거도로 우선순위 평가방법은 지금까지 없었던 생활형 자전거도로에 대한 구체적인 판단기준을 제시하고 정책적 의사결정에 도움을 줄 것으로 기대된다.