마이크로웨이브 균일분산 시스템을 이용하여 자연생태계에 영향을 주지 않고 잡초종자발아를 제어함으로써 토양 및 수질 오염에 영향을 주지 않는 새로운 개념의 제초제 대안을 제시하고자 한다. 명아주(Chenopodium album var. centrorubrum), 쇠비름(Portulaca oleracea)과 한련초(Eclipta prostrata)의 식물에 대한 마이크로웨이브의 토양 함수량별, 깊이별 조사한 결과 균일 분사된 마이크로웨이브를 이용한 잡초 종자의 발아율 측정에서 마이크로웨이브는 토양 함수량에 따른 종자의 발아 조절에는 토양 함수량 20%, 30%에서 최고의 효율이 나타났으며, 토양의 깊이에는 별 영향을 받지 않는 것으로 나타났다. 마이크로웨이브 조사시간은 30초 이상 조사시 잡초 종자의 발아를 억제 할 수 있는 것으로 나타나 적절한 토양 함수량(20~30%) 및 이에 따르는 30초 이상 조사한 마이크로웨이브의 균일분산 시스템을 통해 제초제를 대신하여 잡초종자발아를 조절할 수 있을 것으로 생각된다.

Background : Ginseng seeds are harvested in immature stages and take 3 - 4 month to be germinated following cold stratification. Improvement of germination speed can be a valuable traits to be characterized for economically important medicinal plant. Modulation of cell wall compositions is also considered as another agricultural trait when the crops can be processed for foods and forages. Here we show the functional characterization of ginseng pPLAIIIβ focused on its possible values on germination and modulation of cell wall compositions. Methods and Results : Patatin-related phospholipase AIII family genes were identified from ginseng EST clones and further confirmed by PCR. Clone showing the highest homology with pPLAIIIβ was overexpressed in model plant Arabidopsis, and it displayed dwarf plants. qPCR analysis showed that pPLAIIIβ expressed in all organs of 2-years-old ginseng. Overexpression of ginseng pPLAIIIβ also displayed apparently enlarged seeds as well as altered germination speed in early stage compared to the control. Chemical stainings and direct quantification of lignin and cellulose were performed to understand the link of cell elongation patterns and cell wall compositions. Conclusion : Overexpression of ginseng pPLAIIIβ inhibited longitudinal cell elongation in all tested organs except seeds which is enlarged in both directions than the control. Shorter root length is related with auxin responsive genes and its dwarf morphological phenotype is resulted in altered cell wall compositions.

Background : This experiment was conducted to improve the farm income by decreasing the rate of corrosion and increasing the emergence rate by selecting the proper storage method of the medicinal crop seed root. Methods and Result : Ten kinds of medicinal crop seed roots of Glycyrrhiza uralensis Fischer, Salvia miltiorrhiza Bunge, Codonopsis lanceolata, Platycodon grandiflorum, Atractylodes japonica, Angelica acutiloba, Adenophora triphylla, Rehmannia glutinosa, Angelica gigas and Cnidium officinale were used as test materials. In experiment 1, storage experiments were carried out using four treatments: untreated (dry in advance), untreated, filler (dry in advance) and filler. Drying in advance was carried for 2 days in dark place and the filler was vermiculite. For storage, in styrofoam box, seed roots were laid down in 3 replicates of 10 sets, stored in – 1℃ warehouse. The storage temperature was set as long as each seed root was not frozen investigating freezing point, supercooling point. As a result, the rate of corrosion was significantly lower in the 4th treatments (filler), such as A. japonica (30%) and A. gigas (10%), compared with untreated one. Therefore, in experiment 2, six treatments were carried out using filler. There were 6 treatments: filler disinfectant (dry in advance), filler disinfectant, filler biochar (dry in advance), filler biochar, filler disinfectant biochar (dry in advance) and filler disinfectant biochar. The disinfectant was used by metalaxyl-M at a concentration of 0.4 g/ℓ, and a biochar was used at a volume ratio of 50% (v/v). As a result, the rate of corrosion was significantly lower in the 6th treatment (filler disinfectant biochar), such as S. miltiorrhiza (13.3%) and C. lanceolata (6.7%). Conclusion : In storing medicinal crop root, it is better to store using fillers. It was shown that a mixture of filler and disinfectant biochar reduced the rate of corrosion.

Seeds of colored rice generally have high anthocyanin and flavonoid content but some cultivars have low seed germination rate. This defect poses unstable seedling establishment in nursery bed. The seed gravity variations of 10 colored rice varieties and its effect on germination, seed soaking duration and their optimum seeding rates for seedling raising in machine transplanting were investigated in the experiment. Based on seed gravity distribution of the 7 black- and 3 red-colored rice varieties at three levels of seed gravity (1.0<, 1.0~1.06, 1.06>), the black colored seeds such as Josaengheugchal and Sinmyungheugchal had high amount of specific gravity of below 1.0 ranging from 86~ 96%, while they had only 3~13% of seeds in specific gravity above 1.06. Sintoheugmi, Heugjinju, Heugnam, Heugkwang had various mass of seeds, showing 29~44% for specific gravity of below 1.0, 24~39% for 1.0~1.06 and 25~45% for above 1.06. On the other hand, the red colored rice such as Hongjinju, Jeogjinju and Geonkanghongmi had high percentage of specific gravity of above 1.06 with 84~86% while they had only 9~12% of specific gravity below 1.0 similar to Ilmibyeo of noncolored rice. The black colored seed generally showed low germination percentage, slow germination speed and long mean germination time, and low water absorption rate as compared with seed of the red colored rice which was similar to those of Ilmibyeo used as a control cultivar. The black colored seeds took 2~4 days longer seed soaking duration than the red colored rice before germination. This was related to high seed amount of specific gravity below 1.0 in black colored rices. The high amount of seed gravity above 1.06 in the colored rice seeds was positively correlated with percentage of germination, germination speed and mean germination time, ripened grain ratio and water absorption. Seed gravity distribution effect were less pronounced between back- and red- colored seeds in seedling emergence. The black colored rice has slightly lower normal seedling emergence rate than the red colored rice and Ilmibyeo in the seedbed soil due to high percentage of ungerminated seed. Normal seedling emergence rate of the black colored rice in the seedbed was 75.2~82.2% for 10-day old seedling and 85.3~ 90.9% for 30-day old seedling which was lower by 4.5~ 8.0% and 0~3.3%, respectively, than the red colored rice. Based on the normal seedling number per tray of Ilmibyeo for seeding rate of 10-day and 30-day old seedlings, the recommended seeding rate of black colored rice is 200~ 220g seeds for the 10-day old seedling and 110~130g for the 30-day old seedling in transplanting rice while the seeding rate of the red colored rice was 220g seeds for the 10-day old seedling and 130g for the 30-day old seedling.

Grain color distinguishes between the pigmentation of the outer layer of the kernel. It is known that environmental factors affects the production of anthocyanins and abiotic stresses like high light intensity, low temperature, high salinity and/or drought stress, and others increase their amounts. After 7 days the germination rate between yellow and dark-purple seeds were almost the same with and without stress (100% yellow seeds under stress and without stress germinated, 93.3% under stress and 96.6% without stress of purple seeds germinated), even though at the final stage the germination was almost the same, we can conclude base on our observations that the germination takes place at a different rate. We think that this might be related to the seed color, since the germination of purple seeds under salt stress started earlier than the yellow ones, until both reached the same point. The antioxidant activity was higher in seedlings from dark-purple seeds than the yellow ones, and they were higher under salt stress than without it, supporting our hypothesis that the purple color in wheat seeds works as a protection under salt stress. Furthermore, the qRT-pCR showed that some genes related to the flavonoid pathway were expressed or had more expression in the seedlings from dark-purple seeds than yellow ones.

The objective of this study was comparison of seed yields according to different seeding rates, seeding time and application of chemical fertilizations in Yanji region of China. The experiment was conducted on commercial farmland at Yanji region in 2013. All the experimental fields were designed following randomized block design with 3 replicates. The plant spacing was applied as 65×1 cm distance. To check the effect of seeding rate, two different seeding rate, 0.2 kg/0.1ha and 0.5 kg/0.1ha were applied. The higher seed yield was observed in low seeding rate (0.2 kg/0.1ha). The application of fertilizer (mixture of N and P) showed different results following different experimental fields. However, there were little positive effects following fertilizer application into commercial farmland. When we compare seed yields between two different seeding dates, 4th May and 29th May, the earlier seeding date (4th May) showed higher seed yields. In considering these results, low seeding rate and early seeding time is important for getting high camelina seed yields.

1. 생산지에 따라 벼 종자의 수발아립률 차이가 컸던 호 품벼의 출아율과 발아율을 조사한 결과, 수발아율 8% 인 시료는 수발아율이 1%인 시료에 비해 발아율과 출 아율, 종자활력이 30% 낮았다. 2. 수발아율이 높은 시료는 수발아립의 변색 정도가 심 하고 미발아율과 부패율이 높았다. 수발아립은 배가 돌출하고, 변색된 배유와 곰팡이 감염이 관찰되었으며 배유의 1/2이상이 변색된 종자는 발아율이 매우 낮았다. 3. 종자의 발아 진행정도에 따라 현미의 변색이 심해졌 는데, 싹이 3 mm이상일 경우 배유의 1/2정도가 변색 되었다. 4. 발아 종자는 싹이 3 mm이상일 때부터 재발아율이 크 게 떨어졌으며 퇴화촉진처리 후 발아율의 감소가 더 컸다. 특히 퇴화촉진처리한 발아 종자는 싹이 2 mm부 터 종자활력이 크게 감소하였다. 결론적으로 수발아 발생이 심할수록 파종기 발아율과 출 아율의 감소가 크고, 수발아가 3 mm이상 발생하면 발아율 이 크게 저하될 것으로 생각된다. 또한 수발아는 동일 품종 과 동일 지역에서도 포장 간 차이가 크게 발생할 수 있으므 로 재배관리에 주의해야 한다.

자연상태에서 발아율이 낮은 나도생강은 멸종위기에 처해 1997년 산림청에서 보호종으로 지정되었다. 나도생강의 종자 번식률 향상을 위해 먼저 광, 온도에 따른 발아특성을 알아보았다. 나도생강 종자는 광조건이 암조건보다 발아율이 높고 생육 상태가 양호하였다. 온도별 발아율을 조사한 결과는 20℃에서 발아시키는 것이 다른 온도조건보다 발아율이 높았다. 발아율 증진을 위해 종자 전처리(priming)와 저장방법을 달리하여 실험을 수행하였다. 종자 전처리제로는 1%농도의 KOH와 NaOH용액 30분침지가 효율이 높았고, 1% NaOCl 와 100mg/L GA3용액은 대조구 보다 낮은 발아율을 보였다. 종자저장처리는 건조저장와 습윤저장를 하였으며 저장온도는 두 처리 모두 4℃에서 실시하였다. 습윤저장이 건조저온저장보다 탁월한 결과를 보였으며, 90일간 습윤저장 한 경우 발아율이 95.3%로 건조저장 보다 55.3% 높았다.

콩나물의 생산과정에서 야기되고 있는 부패를 방지하기 위한 다양한 방법이 제시되고 있다. 본 연구는 물에 침종하는 수침(水浸)을 이용한 종자정선과 0.1% acetic acid에 2분간 실시한 종자소독이 콩나물의 발아와 생장, 형태 및 생산수율에 미치는 영향을 조사하고자 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 물에 뜨는 부유 종자의 비율은 5분간 물에 침종시킨 종자를 건져 40분간 aeration 시켜 다시 물에 침종시킬 경우 가장 높았다. 2. 상품으로 판매가 가능한 하배축 길이가 4cm이상의 비율은 은하콩에서는 水浸(水浸)과 acetic acid 처리간 차이가 없었던 반면, 풍산 나물콩과 준저리에서는 acetic acid 처리에 비하여 가결 수침 처리(水浸 處理)에서 높았다. 3. 세근형성 비율과 개체당 세근수는 은하콩과 준저리에서는 수침 처리(水浸 處理)에서, 풍산나물콩은 acetic acid 처리에서 상대적으로 높고 많았다. 4. 개체당 전체생계중은 은하콩과 풍산나물콩에서는 수침 처리(水浸 處理) 보다는 acetic acid 처리에서 많았던 반면. 준저리는 이와 반대의 결과를 보였다. 그러나 준저리를 이용한 대량생산 체계에서의 생산 수율은 수침(水浸)을 이용한 종자정선과 acetic acid를 이용한 종자소독간에 차이가 없었다.

The film coated snap bean (Phaseolus vulgaris) seeds with five different coating materials treated with 3% increase in seed weight were planted at sandy loam soil controlled moisture content of 18, 19, 20 and 21 %. The oxygen diffusion rate (ODR) was calculated from the different moisture content soil. The number of normal seedlings, seedling vigor, and seedling capability in field (seed vigor x dry matter weight) were observed at 9 days after planting and compared to the changes of ODR. The germination rate and ODR were sharply decreased simultaneously in the seeds planted at 21 % soil moisture content. Seedling emergence did not occur at all as the soil moisture content increased above 22 %. Hence this value should be considered as the threshold of soil moisture content for seedling emergence. An ODR value under 20% did not influence the percent emergence significantly. The certain difference observing in the emergence at the same ODR was not related clearly to the condition of soil. So it can be assumed that the limit of soil moisture content for the emergence of snap bean was approximately 20%. The value of 18% soil moisture content may be considered as the optimum for snap bean emergence. There was close relationship between the mean value of ODR in different soil moisture contents and the emergence. The germination rates of the seeds coated with the different materials were quite different when the seeds were planted at 21 % soil moisture. Dry weight of the seedlings from film coated seeds was decreased slightly, but the germination rates were not much different from the non-treated control under relatively higher soil moisture content (21 %). Major factor lowering emergence rate was oxygen stress while film coating act as a minor constraint for snap bean sown in excess soil moisture condition.

가시오갈피의 종자번식에서 가장 큰 문제점 중의 하나인 종자의 불량과 발아가 동시에 이루어지지 않는 문제를 해결하고 최대한 많은 묘목을 얻기 위해서는 파종시기의 선택이 중요하다. 파종 전 종자처리를 통하여 성숙정도가 다른 가시오갈피의 파종 후 유묘출현율과 최종생존율을 높이기 위한 기초자료를 얻기 위해 본 연구를 실시한 결과를 요약하면 다음과 같다. 1. 15℃에서 145일 충적 저장한 가시오갈피 성숙종자의 개갑율은 16.6%로 매우 저조하였다. 2. 개갑종자의 기내발아실험에서 GA3처리는 최고로 26.6%의 발아율을 보였으나 발아는 동시기에 이루어지지 않았다. 3. 후숙처리후 10일간 저온 처리한 개갑종자에서 파종 전 GA3처리는 유묘출현율을 높이는 효과가 있었으나 저온처리는 대체하지 못하였다. 4. 휴면타파를 위한 저온 충적저장전의 종자의 GA3처리는 파종 시 개갑종자의 유묘출현율에 영향을 주지 못하였다. 5. 저온저장 40일 경과한 후 파종 전 GA3처리는 비개갑종자에서는 유묘출현율과 생존율을 높였다. 개갑종자에서는 유묘출현율은 14.6% 증가하였지만 최종생존율은 오히려 9.4% 감소하였다.

호밀 적ㆍ만기 파종에서 종자성숙 및 파종량이 출아, 생육, 청예 건물수량 및 종실수량에 미치는 영향을 구명하고저 경북 경산군 영남대학교 농장에서 재래종(팔당호밀)을 공시하여 파종기 10월 28 일(적기) 및 11월 11일(만기)로 하고 파종기별로 출수후 45일 및 30일에 수확한 종자를 파종량을 달리하여 ( 1m2 당 300입, 450입, 600입, 750입, 900입) 시험을 실시하였다. 출아수 및 분얼수는 적기파종에서 출수후 30일과 45일 종자간에 차이가 없었고, 파종량을 증가할 때 출아수가 증가하고 분얼수는 감소하는 경향이 있었고, 만기파종에서는 출수후 45일 종자에서 30일 종자보다 출아수와 분얼수가 많았으며 파종량 증가에 따른 차이는 인정되지 않았다. 만파에서 출수후 30일 종자는 파종량 증가로서 적정주수 확보가 어렵다. 출수기는 적ㆍ만파에서 출수후 30일 종자가 2~3 일 늦었다. 청예수양은 만파에서 크게 감소하였고 적ㆍ만파모두 파종량을 높일 때 청예수량도 증대되어 적기파종에서 750립 / m2 ( 15kg/ 10a)의 파종량에서 최대가 되었고, 출수후 30일 종자는 만파에서 현저하게 낮았다. 수수 및 1000입중은 적기파종에서 만기파종보다, 그리고 만기파종에서는 출수후 45일 종자로서 파종량 750립/m2 ( 15kg/10a) 이상에서는 적기파종의 종실수량 수준까지 접근할 수 있었다. 출수후 30일 종자는 만기파종에서 파종량을 증가하여도 출아율이 낮고 청예 및 종실수량이 심하게 감소되어 종자로 이용하기는 불안정하다.