This study was conducted to investigate the effects of sowing density, number of seeds sown per hole, andthinning treatment on growth characteristics and disease occurrence in Panax ginseng under direct sowing cultivation in ablue plastic greenhouse. Seedling were grown from 2 or 3 seeds sown, and the healthiest was only retained, while the restwere thinned out at the foliation stage. NO3-N, P2O5, and organic matter content differed significantly between growthconditions in the plastic greenhouse and in conventional shade in the soil. Disease also tended to be higher in the conven-tional shade than in the plastic greenhouse. Plant height and stem length showed an increasing trend with increasing sowingdensity and number of seeds sown per hole. However, these measures noticeably decreased when thinning treatment wasconducted. Growth of the subterranean part of ginseng was not markedly influenced by sowing density, the number of seedssown per hole, or thinning treatment. Root weight, which is an important factor in yield, was significantly affected by thenumber of seeds sown and thinning treatment. Interestingly, root weight tended to be higher in the thinning treatment plotthan the untreated control plot. Damping-off and root rot increased noticeably as the number of seeds sown increased. Dis-ease also tended to be substantially higher in the thinning treatment plot than the untreated control. However, physiologicaldisorder of the plants did not vary with sowing density, the number of seeds sown, or thinning treatment.

The influence of seed maturity, stratification, and seed washing method on seed germination were investigated for establishment of seed propagation system in dropwort (Oenanthe stolonifera DC). Seed germination rate was increased as period of the seed ripening increased, especially the seed over 40 days after flowering showed the highest germination rate. The optimum period of cold stratification treatment to promote seed germination was about 8 weeks. The seed treated by a cold wet stratification with sand exhibited higher germination rate compared to the seed treated by a cold wet stratification without sand. The stratified seeds that were stored in the ground having fluctuating temperature were improved up to 19%, while stratification stored under constant temperature(4℃) did not improve germination rate. In addition, 8 weeks of stratification period under fluctuating temperature in the ground was most effective. Sodium hypochlorite, vital oxide, and distilled water were used as chemicals to remove the germination inhibitor of seed coat. Water washing of seed coat after soaking in 0.5% sodium hypochlorite and 0.0005% vital oxide for three minutes and 60 minutes respectively could effectively remove the germination inhibitors in the seed coat.

Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

The objective of this study was comparison of seed yields according to different seeding rates, seeding time and application of chemical fertilizations in Yanji region of China. The experiment was conducted on commercial farmland at Yanji region in 2013. All the experimental fields were designed following randomized block design with 3 replicates. The plant spacing was applied as 65×1 cm distance. To check the effect of seeding rate, two different seeding rate, 0.2 kg/0.1ha and 0.5 kg/0.1ha were applied. The higher seed yield was observed in low seeding rate (0.2 kg/0.1ha). The application of fertilizer (mixture of N and P) showed different results following different experimental fields. However, there were little positive effects following fertilizer application into commercial farmland. When we compare seed yields between two different seeding dates, 4th May and 29th May, the earlier seeding date (4th May) showed higher seed yields. In considering these results, low seeding rate and early seeding time is important for getting high camelina seed yields.

This study was conducted to improve the postharvest storage techniques of managing and storing seeds, totest qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthesjaponica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperaturesand durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germinationrates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than thosein Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12㎜ than those in Je-Cheon about 4.90㎜ andseed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areaswere higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing tempera-tures and durations, they were not clearly different in between 4℃ and 25℃ and they also were gradually decreased withgetting longer storing durations. The germination rates treated by plant growth regulators were higher with GA3 than thosewith Kinetin. The highest seed germination rate was appeared at 50 ppm of GA3. Comparing its rates with different seedpriming, they were relatively higher with KNO3 than those with PEG6000. In protein expression patterns between before thegerminating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination com-pared to those before the germination of seeds, especially 10~20kDa. These results showing more and clear bands weremore clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulatorsand seed primings, GA3 was better expression than those with Kinetin and KNO3 was better than those with PEG6000. Moreand clear bands were closely related to the germination rates of seeds and more detailed studies would be required.

Characteristic of seed for Sageretia thea fruits collected from the habitat of harvest season in Jeju and theirgermination ratio in different temperatures were considered. The average weight was 0.2g, average diameter was 7.2㎜,average length was 6.5㎜, and size distribution range was 5.1~10.0mm. The number of seeds per fruit was 1.8, and 1000grain weight was 7.77g with diameter of 3.7㎜ and thickness of 1.7㎜ in size. The fruit maturation was investigated to befrom April 27 to June 1, when the best maturation period was about a week, May 11 to May 18. Also, the fruits grown onMay 4 to May 11 were seen to have the bigger in transverse size and weight with a tendency of the earlier maturation datehas the greater the number of seeds. S. thea seeds pretreated for 24 hours at 50℃ were not germinated, while most of thosepretreated for 24 hours at 4℃ were successfully germinated at any degree of temperature (except at a temperature of 10℃).Particularly, the highest germination rate of 55% was made at 15℃, and plumule and radicle were best grown within thetemperature range of 25℃.