Background : Acanthopanax divaricatus var. is a Korean woody natural plant which can grow in the field placed in low altitude. Cutting is more efficient for propagation than seedling since it takes 3-years to harvest seeds and get seedling. This study was carried out to find out efficient cutting propagation method. Methods and Results : Hardwood cutting (April 10) and semihardwood cutting (September 7) was performed to accelerate cutting propagation rate on 5 type nursery box soil composed of Sand, vermiculite, peat moss, perlite, and perlite + peat moss. In order to verify growth regulators on cutting propagation, rooton, IBA 2,000 ppm, IBA 3,000 ppm, NAA 500 ppm, NAA 1,000 ppm were treated and cutting performed in sand nursery box soil on March 30 and September 7 with 3 replications of 50 plants. In terms of rooting traits of cutting in April 10 according to nursery box soil, rooting rate was higher as 67% in sand and lower as 11% in peat moss. Root length, root number, and root weight was also higher in sand. In terms of rooting traits of cutting in September 7, rooting rate was higher as 60% in sand and root number was higher in sand and perlite. Root weight was higher in perlite. The result of growth regulator effect on cuttings was as follows. Rooton increased rooting rate higher as 68.7% in cutting on March 30 and IBA 3,000 ppm also increased higher root length, root number, and root weight as 5.4 ㎝, 12.3 ea/plant, 3.13 g/10plant respectively. rooton increased rooting rate higher as 67.3% in cutting on September 7 as same as cutting on March 30 with no significant difference and root length, root number, and root weight were all higher in rooton treatment too. Conclusion : The result of cutting treatment in 5 type nursery box soil on April 10 and September 7 to accelerate cutting propagation rate of A. divaricatus var. shows that sand was most efficient with higher root length, root weight, and rooting rate. Most efficient growth regulator for rooting was rooton with higher rooting rate and better rooting traits altogether same in cuttings on March 30 and September 7.

Background: Planting vigorous cuttings that quickly develop shoots and roots is essential to the biological and economic success of producing medicinal flowers. The present study aimed to evaluate the effect of storage temperature and duration on seedling capacity in the propagation of Chrysanthemum indicum L. and to investigate the effect of rooting media on the growth of C. indicum L. after cutting.Methods and Results:Returning cuttings to supplemental cold storage (2.0 ± 1.0°C) may extend duration of cutting viability 6 weeks, returning cuttings to supplemental warm storage (25.0 ± 1.0°C) is not recommended. The treatment of the growing media experiments, which were conducted in the 2014 planting seasons, included sawdust, river sand, topsoil + sawdust, topsoil + poultry manure, sawdust + river sand, river sand + poultry manure, topsoil + river sand + poultry manure, topsoil + poultry manure + river sand + sawdust. Result indicated that the topsoil + poultry manure media performed best and supported the highest number of branches (3.47), branch length (26.39), and number of leaves (88.63).Conclusions:The results of the present study suggest that cold storage and the topsoil + poultry manure growth media was superior in supporting the early establishment of C. indicum cutting, this result will have a tremendous influence on propagation of this species.

Background : This study was carried out to survey the effect of IBA (indole-3-butric acid) concentration on root development characteristics in Viburnum dilatatum cuttings. Methods and Results : The cuttings were collected on July 28, 2016 from plants growing in Korea National Arboretum. Cuttings were treated with different IBA concentrations [0 (control), 10, 100, 1000, 2000 ㎎⋅L-¹] for 10 seconds. Rooting parameters (root number, the longest root length, root fresh weight, root dry weight, rooting percentage, and survival rate) were observed at 8 weeks after cuttings. Rooting percentage was 43, 33, 17, 47, and 40 % at 0, 10, 100, 1000, and 2000 ㎎⋅L-¹ of IBA, respectively. Root number (13.4) was highest under 1000 ㎎⋅L-¹ IBA. Root length (9.4 ㎝) and Root weight (fresh and dry) were highest under 1000 ㎎⋅L-¹ IBA. Conclusion : According to the results, 1000 ㎎⋅L-¹ IBA was the best treatment for increasing percentage rooting percentage and other root development characteristics of V. dilatatum cuttings.

This study was conducted to investigate the effect of shading degree and rooting media on the growth of Caragana sinica and Sedum middendorffianum after cutting. In C. sinica, the highest rooting rate was obtained in cuttings planted in horticultural soil (Sunshine Mix #1) and peat moss mixture (peat moss : perlite = 1 : 1, v/v) under one layer of 35% shading and in cuttings planted in kanumatsuchi soil mixture (kanumatsuchi soil : decomposition of granite = 1 : 1, v/v) under non-shading. Whereas, regardless of shading degree, most cuttings of S. middendorffianum rooted in both horticultural soil and peat moss mixture. Cuttings of C. sinica showed the highest root length, 10.4㎝ in kanumatsuchi soil mixture under one layer of 35% shading but the highest fresh and dry weight of roots in kanumatsuchi soil mixture under non-shading. In S. middendorffianum, the highest root length, fresh and dry weight of root were obtained in cuttings planted in horticultural soil under non-shading. With these results, we recommended that cuttings of C. sinica should be propagated in kanumatsuchi soil mixture under non-shading and cuttings of S. middendorffianum in horticultural soil under non-shading.

본 연구는 제주특산식물인 좀향유의 현지 내･외 보존을 위한 기초 연구로서 증식체계를 마련하고 전처리제 처리에 따른 발 근 특성과 시비에 따른 유묘의 생장 효과를 등을 알아보았다. 온 실과 밀폐상의 온습도 조사결과 평균 온도는 비슷하였으나 공 중 습도는 온실보다 밀폐상이 높은 습도를 유지하였으며, 좀향 유 삽목 후 온실은 10일, 밀폐상은 7일만에 최초 발근을 확인하 여 밀폐상의 빠른 발근율을 보였으며 온실과 밀폐상에서의 생 존율, 발근율, 뿌리수 등은 근소하게 밀폐상이 높게 조사되어 온실보다 밀폐상이 효과적이었다. 발근억제물질 제거를 위한 전 처리제 처리는 AgNO3 77.5%를 제외한 다른 처리구에서 95% 이 상의 높은 발근율로 조사되어 좀향유의 경우 발근억제 물질은 적은 것으로 판단된다. 유묘의 용기별 질소 시비량 및 Osmocote 시비시험 결과 시비량과 용기의 조건에 따라 생존율과 생장 등 에 차이를 보였다. 배양판에서 완효성 비료인 Osmocote를 시비 한 결과 생존율, 지상부, 지하부 생장 등이 가장 양호하여 좀향 유의 유묘 증식을 위해서는 Osmocote 시비가 바람직 할 것으로 판단된다.

This experiment was carried out to establish the optimum cutting time, plant growth regulator, and bed-soilfor rooting by greenwood cutting of Schisandra chinensis. Approximately 7㎝ long-shoots of greenwood cuttings weretransplanted by various cutting times, plant growth regulators, and bed-soils in the plastic-film house. The rooting rate ofgreenwood cutting was 12.5% in the April 5th, 73.5% in the May 8th, and 75.5% in the August 5th. The number and lengthof primary root in greenwood cutting were more in the early May than those in the early August. For mass propagation ofSchisandra chinensis. using greenwood cutting, shoots were treated with plant growth regulators on May to increase rootingrate. Rooting rate was 100% with IAA 50㎎/ℓ,92.9% with NAA 100㎎/ℓ,and NAA 1,000㎎/ℓ,for 60 min. To selecteffective media for rooting, various medias for bed-soil were treated by single and mixture form for 100 days after cutting.Rooting rate was 91.8% in the single treatment of peat moss or decomposition of granite soil, and this result was better thanthose in other treatment. The treatment by 1:1 mixture of peat moss and horticulture bed soil was rooting with 94.0% bestrooting rate.

사시나무의 클론간 생장 특성(전체 건중량, 줄기 건중량, 잎의 전체 건중량)과 대사물질간의 관계를 구명하여 조기선발을 위한 생장 관련 주요 인자를 구명하고자 사시나무 삽목묘(유묘)를 대상으로 우량클론과 일반클론간의 생장특성을 조사하였고, 수체내의 대사물질을 분석하였다. 사시나무 유묘의 줄기 건중량과 잎 내의 glucose, sucrose, inositol 등 당 함량과 정의 상관관계(p<0.05)가 있었으며, 잎의 건중량과는 줄기 내의 glutamic acid, alanine, threonine등과 같은 아미노산류의 함량과 정의 상관관계가 있었다. 사시나무 잎 내의 대사물질은 우량클론에서 glucose, sucrose, inositol 등과 같은 당류가 일반클론보다 고도의 유의 차이(p<0.01)가 있게 높게(1.3배 이상) 함유한 것으로 나타났으며,rythronic acid, malic acid, pentanedioic acid 및 proline도높게 함유한 것으로 나타났다. 또한 우량클론의 줄기내의 대사물질은 glutamic acid, alanine, threonine등과 같은 아미노산류의 함량이 일반클론에 비해 유의적으로 높게(1.2배 이상)함유하고 있는 것으로 나타났고, 더불어 succinic acid, maleicacid의 함량도 고도의 유의차이(p<0.01)로 우량클론에서 높게 함유하고 있었다. 생장이 우수한 사시나무 클론은 유묘의잎에서는 당류가 줄기에서는 아미노산류가 높게 함유하여 이러한 복합적 인자가 사시나무 우량클론 선발을 위한 대사 표지자의 개발에 필요한 기초 자료로 이용될 수 있을 것이다.

This study was conducted to enhance the propagation of Acanthopanax divaricatus and Acanthopanax koreanum through different cutting practices. For propagation by cuttings, scions were taken on September 1. This already has hard skin and showed much higher values in terms of root length, root number, rooting ratio and root weight than those taken on June 30 and August 1. Regardless of cutting date, 50% shading resulted to the highest root length, root number, root ratio and root weight. Meanwhile, 95% shading significantly reduced these parameters compared with no shading. These results suggest that over-shading may inhibit root growth. Two growth regulators, IBA (concentration of 1,000, 2,000, 3,000 ppm) and Rootone-F (0.4%) were also tested of its effect to the cuttings. Rootone-F was found to be more effective than IBA. Cutting treated with Rootone-F had slightly higher root length and root number. A. koreanum which grows well in hot climatic condition showed better rooting ability than A. divaricatus.

눈향나무(Juniperus chinensis var. sargentii Henry)는 누워서 자라는 상록침엽관목으로 수고 60 cm까지 성장한다. 지리적으로는 동북아시아에 한정되어 분포하는 수종이며, 우리나라에서는 고산지대의 산정부근에서만 극히 일부 자생하여 멸종의 위기에 직면하고 있는 위기종(Endangered species)으로 지정되어 보호되고 있다. 본 연구는 희귀수종 눈향나무의 유전자원보존을 위한 삽목증식법을 개발하기 위하여 삽목시기 및 발근촉진제의 종류별 농도에 따른 발근특성을 조사하였다. 삽목 발근율은 삽목시기와 발근촉진제의 종류 및 농도에서 각각 고도의 유의성이 인정되었다. 삽목은 8월보다 5월이 적정한 시기로 판단되었으며, 발근율과 발근특성을 고려한 발근촉진제의 종류 및 농도는 IBA 1000 mgL-1 처리하는 것이 발근율 36.4%(무처리 30.4%)로 캘러스 형성율이 높고 발근이 촉진되는 것으로 나타났다. 삽수의 발근에 따른 뿌리의 수, 길이 및 굵기에 대한 특성에서는 모두 발근촉진제의 종류 및 농도에 따른 통계적인 유의성이 인정되지 않았다.

억새는 우리나라 등 동아시아가 원산인 C4작물로서 연간 바이오매스 수량이 30톤/㏊ 이상으로 많아 유망한 에너 지작물이지만 대량증식기술이 개발되지 않아 재배면적 확대가 어렵다. 본 연구는 억새의 줄기삽목에 의한 대량 증식기술을 개발하기 위해 수행하였는데 그 결과를 요약하면 다음과 같다. 삽수의 유형은 마디에 신선한 잎집이 남아 있는 것을 어린 삽수로, 잎집이 고사되었거나 소멸된 것을 성숙삽수로, 마디의 눈에서 새싹이 신장하였으나 새잎이 완전히 전개되지 않은 것을 신초신장 초기 삽수로, 마디의 눈에서 새싹이 신장하여 새잎 이 전개된 것을 신초신장 후기삽수로 구분하였다. 어린 삽수 및 성숙 삽수의 발근율은 각각 47%, 70%이었으나 신초신장 초기 및 후기 삽수는 각각 94%, 100%로 높았다. 억새 줄기 삽목시 발근율이 높고 묘 소질이 좋은 신초신장 삽수를 얻기 위해 줄기의 상단부를 절단하여 14일 후에 절단하지 않은 줄기와 신초신장 삽수 수를 비교한 결과 상단부를 절단하지 않은 줄기에서는 신초신장 삽수를 1개만 얻을 수 있었으나 절단한 줄기에서는 6개로 신초신장 삽수가 증가하였다. 억새 대량 증식을 위한 줄기 삽목방법의 다양한 억새 종에 대한 적용 가능 성을 구명하기 위해 일반 물억새(M. sacchariflorus), 3배체 억새(M. X. giganteous), 거대억새 1호(M. sacchariflorus)를 삽목하여 발근 및 지상부 생육양상을 비교한 결과 3배체 억새와 물억새 모두 발근율이 100% 인 점에서 거대억새 1호와 차이가 없었다. 따라서 억새 대량 증식을 위한 줄기 삽목방법은 거대억새 1호, 물억새 뿐만 아니라 바이오매스 수량은 많지만 종자번식이 불가능한 3배체 억새 증식에도 이용할 수 있을 것으로 판단된다.

This study was conducted to survey the habitat environment as draft data for restoration and massive propagation method of Forsythia saxatilis were classified as rare species and disappear in Seoul. The light, soil environment in habitat was surveyed and the cutting experiment as species, cutting periods, and rooting promoter was conducted to know physiological characteristic of Forsythia saxatilis The results, light was low to forest tree and soil in the native site indicated that pH 4.78～5.05, EC 0.04～0.05 dS․m-1, OM 1.23～3.27%, Ca 4.21 mg․kg-1, K 0.41mg․kg-1, Mg 0.69 mg․kg-1. 0.01 % N, 0.44mg․kg-1 P showed low and 12.87～23.56cmol+․kg-1 CEC. It was very poor soil environment to live plant as general forest soil. In Propagation, Rooting rate showed hardwood 90% and softwood 100% in summer and softwood 90% in summer and 79% in spring. Rooting rate was showed of each species, Forsythia viridissima 100%, Forsythia ovata Nakai. 80%, tetraploid 60%, and Forsythia saxatilis 90%. There is no effect of rooting promoter treatment. We gained 120 plants by cutting propagation and renaturated to habitat. It is the reason that Forsythia saxatilis disappear at habitat by have no seed and low adaptation of environmental change. To massive propagation by cutting, it is important to control cutting periods rather than rooting promoter treatment.

In this study, for mass cultivation and standardization of hydrangea, experimental result for types and concentration of growth regulator appropriate for cutting and rooting are as follow. During the semi-hardwood and hardwood cuttings of hydrangea, in 100mg·L­¹, 500mg·L­¹, 1,000 mg·L­¹ of each growth regulator NAA and IBA, when sudden soaking procedures were conducted the hardwood cutting rather than the semi-hardwood cutting resulted better growth as well as significantly better to initial rooting. The treatment of IBA was better then NAA in the rooting, especially IBA was the rooting percent increased at 500mg·L­¹and root growth was effective at 1,000 mg·L­¹also. It is effective to root growth in NAA 1,000mg·L­¹and percent of rooting increased under high 1,000 mg·L­¹ and it was more effective and higher concentration.

The cutting propagation of Illicium religiosum Sieb. et Zucc was effective such as hardwood cutting was done in May and softwood cutting was done on in July. The optimal soil composition for rooting was 1:1:1(vermiculite, peatmoss and perlite). The result was that the softwood cuttings done in July took roots in every bed; especially, cuttings in the bed processed with 1,000 mg/L IBA showed the best result: 90% of rooting rate, 13.5 in number of rooting, and about 8cm of root length. Hardwood cutting done in May showed that the bed processed with 1,000 mg/L IBA resulted in 90% of rooting rate; the bed processed with 1,000 mg/L IBA + 1,000 mg/L NAA showed 80% of rooting rate; the number of main roots and the length of roots were also satisfying. For the cuttings of I. religiosum "Variegata" Sieb. et Zucc, the result was less satisfying than that of I. religiosum Sieb. et Zucc, but it was proved that even the cuttings of “Variegata” took roots in the bed processed with 100 mg/L IBA and 1,000 mg/L/ IBA.