Since 1982, female agricultural workers are populous than male in Korea. Previous study was suggested that female workers are more sensitive to the agricultural medicine poisoning than male. This study was conducted with the aim of evaluating the impact on plasma components and nutrient intakes in female agricultural workers by the use of pesticides. Data were obtained from 44 females residing in Wonjoo, Kangwon-do area. Analysis for the general characteristics, nutrient intakes, and plasma components of the subjects were performed by physical examination, 24-hour recall method and venous blood sampling. They were divided into 3 groups according to the degree of the pesticide use: 8 in none(Non-expose group), 14 in low(〈4/yr)(Low-expose group), and 22 in high(≥4/yr)(High-expose group). The results are summarized as follows. Serum glucose, SGOT, SGPT, triglyceride, HDL-cholesterol did not correlated with pesticide use and were not different among the groups. But serum total cholesterol was higher in pesticide use group. With increasing the number of pesticide use times, leukocyte count was tended to increased. Eighty two percent of the subjects were hemoglobin less than or equal to 12g/dl. Hematocrit, MCV, MCH, MCHC, serum Fe, Serum ferritin belonged to normal range and did not correlated with pesticide use. Intake of energy, Fe, vitamin B2 were lower than RDA. Fe, K, and vitamin A intake were higher in don't use group than the other groups. There are not many differences according to using the pesticide in anthropometric measurements, nutrient intakes, and blood components, except for serum total cholesterol level and some nutrients intake. But these results suggest the need the systemic researches about the effects the pesticide using on nutritional status in Korean agricultural workers.

Microwave-assisted extraction (MAE) is known as a more environmental-friendly process with economic advantages in terms of less time, less solvent, less energy and less waste than the current reflux method that is time-consuming. MAE was applied to extract three pesticides (quintozene, tolclofos-methyl and procymidone) from ginseng in a pesticide residue analysis and eliminate pesticide contaminants from ginseng prior to production of the ginseng extracts. The optimal conditions for pesticide extraction from ginseng were 50% in power (150 watts), 2 minutes, 20 mL hexane and 2.0 g sample. The results of optimal conditions were compared with those of Soxhlet method (7 hours, 150 mL hexane). The recoveries between two methods were almost same while time and amount of solvent used in MAE were significantly decreased in comparing with those in the Soxhlet methods. When the 45% ethyl alcohol as a extraction solvent was applied to eliminate pesticides from ginseng prior to the production of the ginseng extracts, it was found that 100% quintozene, 69% tolclofos-methyl and 83% procymidone were eliminated from ginseng. The data showed that MAE may be used to eliminate pesticide contaminants in ginseng prior to making the ginseng extracts.

Limited information is available on the acceptability of Korean MRLs(maximum residue limits) and the health risk based on the pesticide exposure by food intake. The aim of this study was to evaluate TMDI(theoretical maximum daily intake) and EDI(estimated daily intake) for Korean by using MRLs, food intake, residue data, and correction factors, and compare with ADI(acceptable daily intake) in order to estimate the health risk based on the pesticide exposure. The study was performed in three steps. In the first step, the residual pesticides in each category of food were investigated using the pesticide residue analytical data(1995-96) from officially approved organizations and the analytical data for poultry was adopted from Korean food code method. In the second step, TMDI was estimated from MRLs and food factors, and was compared with ADI. In the third step, the effectiveness of each culinary treatment (washing, peeling, steaming, boiling, and salting) was evaluated and EDI was calculated using pesticide residue data, food factor, and correction factor by treatment. TMDI obtained from MRLs and food intake, and food intake was summed as 1,100.99 g, which was 79.1% of total consumption. The percent ratio of TMDI to ADI for 156 pesticides was mostly below 80% and only 30 pesticides exceeded the ADI. In particular, non-treated EDI from pesticide residue data and food intake was summed up to about 43 ug/day/capita, and the rank was procymidone(8.6 ug) $gt; malefic hydrazide(8.2 ug) $gt; EPN(3.7 ug) $gt; deltamethrin(3.5 ug) $gt; cypermethrin(3.0 ug). The treated EDI calculated from pesticide residue data, food intake, and correction factor by culinary treatment was summed up to 13.7ug/day/capita. The percentage of ADI was TMDI(79.74%) $gt; non-treated EDI (0.17%) $gt; treated EDI(0.04%), and the exposure level of Korean population to whole pesticides was below the level to produce health risk. Oncogenic risk of five pesticides used in Korea whose oncogenic potency(Q*) was known were assessed from TMDI and treated EDI. Dietary oncogenic risk for Korean was estimated to be 2.0 × 10^(-3) on the basis of TMDI, 8.3 × 10^(-7) on the basis of treated EDI. The oncogenic risk from TMDI exceeded the risk level(1 × 10^(-6)) of EPA, whereas the oncogenic risk from treated EDI and real exposure level was lower than that of EPA.

Effect of storage temperature, washing, and cooking on postharvest-treated pesticide residues in polished rice was investigated. After being traeted with each 500 mg/kg of captan, carbaryl, phenthoate, fenthion, fenitrothion, chlorpyriphos-methyl, pirimiphos-methyl, the polished rice was stored for 8 weeks at 4 and 30℃, respectively. The penetation rate of carbaryl was the highest as 27.5% and the others, 13 to 18%. The half lives of pesticide residues were estimated as 30 to 230 weeks at 4℃, but 1 to 12 weeks at 30℃. The residues were reduced faster at 30℃ than at 4℃. The half lives of pesticide residues by water washing were estimated as 0.7 to 4.6 trials and the residues were removed with the washing trials. The residues of captan and carbaryl in cooked rice were removed 100 and 98%, respectively, comparing to initial residues concentration in treated rice but those of other 5 pesticides were removed 80%.

살충제와 방사선이 자주달개비 수술털 돌연변이에 미치는 복합적 영향을 평가하였다. 포트에서 생육된 Tradescantia 4430 식물체에 코발트 선원으로부터 0.3, 0.5, 1.0 및 2.0 Gy의 감마선을 조사하였다. 살충제는 감마선 조사 24시간 전에 농업용으로 사용되는 파라치온 유제를 살포하였다. CT 실험군과 Pa+γ실험군에 있어서의 돌연변이 빈도가 증가한 고조기간은 방사선 조사 후 7 ~ 11일로 돌연변이 빈도는 방사선량 증가

An attempt was made to determine the residual distribution of organochlorine and organophosphorus pesticides in the various kinds of Korean tea which were purchased from the market. The organochlorine pesticides investigated in this study were BHC, DDT and dicofol and the organophosphorus pesticides were diazinon, EPN, fenitrothion and parathion. The pesticide residues were determined by GC-ECD and NPD. Only BHC was detected in all the samples and it's level were ranged from 0.00064 ppm to 0.05995 ppm and it's average was 0.00682 ppm and DDT, dicofol and organophosphorus pesticides were not detected in all samples. The organophophorus pesticides were detected(0.0035-0.0983 ppm) in raw materials but were not in the manufactured material and it is considered that the largely components of the pesticides is removed by drying and high temperature while the tea was manufactured. The recovery tests of the pesticides gave satisfactory results showing an average yield of 97.6% with organochlorine pesticides and 92.5% with organophosphorus pesticides and the detection limits level were 0.00008 ppm to 0.0010 ppm.

This study was carried out to determine the pesticide residues in rice bran, crude rice bran oil and the oil of various stages of refining process. Each samples were analyzed for 41 pesticide residues by multiclass multiresidue methods with GC-ECD, NPD and identified by GC-MSD. Rice bran were detected cypermethrin, diazinon, dichlofluanid, and its level were ranged from 0.01-0.122 ppm. Crude rice bran oil were detected cypermethrin, diazinon, dichlofluanid, dimethoate, etrimfos, flucythrinate, and its level were ranged from 0.015-0.654 ppm. Crude rice bran oil has the higher level of pesticide residues and more varieties of pesticides than rice bran. But pesticide residues in the crude rice bran oil was found to be almost removed when pigment was decolorized by absorption using active carbon and clealy removed by thermolysis for deodorization.

Resistance evolution to organophosphate-based pesticides in apple and pear inhabiting arthropods of western North America extends to many classes of pest and some beneficial species. Resistance management programs to minimize resistance in pests while exploiting it in natural enemies have met with mixed success. Among beneficials, resistances have been exploited mostly among predators of pest mites. Evolution of resistant mites, leafminers, leafhopper, aphids, leafrollers and some internal fruit feeders have led to development of new monitoring methods and means to delay or avoid resistance. But it is resistance to azinphosmethyl in codling moth (Cydia pomonella) that is changing the pest control system and moving it from chemical to biologically-based means. Newly merging IPM system will depend more on use of biological, cultural, behavior and genetic controls. But more selective pesticides also will be needed to augment pheromones, resistant host plants and genetically altered organisms. These more biologically-based tactics will be prone to resistance evolution in pests as well, if used too unilaterally and/or too extensively.

In general, herbicides have been classified according to selectivity, mobility. time of application, methods of application, mode of action and chemical property and structure. However, there was no generally accepted classification system for practical use in the field. The primary processes affected by the majority of herbicides are the growth process through cell elongation and/or cell division, the photosynthetic process specifically the light reaction, the oxidative phosphorylation and the integrity of the membrane systems. The usual approach in the study of the mechanism by which herbicides kill or inhibit the growth of plants is to initially determine the morphological phototoxicity systems, The mechanism by which a herbicide kills a plant or suppresses its development is actually the resultant effect of primary and secondary(or side) effects. In most instances, the death of the plant is due to the secondary effects. To induce the desired response, a herbicide must be able to gain entry into the plants and once inside, to be transported within the plant to its site(s) of activity in concentrations great enough. Obstacles to the entry and movement of herbicides in plants are generally classified by leaf and soil obstacles, translocation obstacles and biochemical obstacles, and these obstacles are also strongly influenced by plant species and by environmental factors such as light, temperature, rainfall and relative humidity. And hence, in most instances, results obtained from laboratory or greenhous vary from those of field experiment. Author attempted to classify herbicides from the field experiment using the two-dimensional ordination analysis to obtain practical information for selecting effective herbicides or to choose effective herbicide combinations for increasing herbicidal efficacy or reducing the chemical cost. Based on this two-dimensional diagram, desired herbicides or combinations were selected and further investigated for the interaction effects whether these combinations are synergistic, additive or antagonistic. From the results, it was concluded that these new approach could possibly be give more comprehensive informations about effective use of herbicide than any other systems.

Under the limited arable land, the enhancement of agricultural productivity is indispensable to provide the food demand which is concomitant with the rapid increase in population. From this viewpoint, the upbringing and dissemination of high-yielding varieties has been promoted continuously and several modifications in cultural practices, including heavy fertilization, dense planting, and early transplanting, also have been gradually developed. However these changes in cultivation have led to the increased outbreak of insect pests and diseases. And this unexpected results have accelerated the number and complexity of pesticides employed as well as their consumption. Even though pesticides are essential materials contributing to the steady production of agricultural crops, large scale consumption of them has given rise to several adverse impacts, such as mammalian hazard and/or environmental contamination. In this respect, recent development of new pesticides has been concentrated on 'safe pesticide', as it were, that has the highly selective properties without unfavorable side influences on other ecosystem. According to literature cited up to now, feasibilities of safe pesticide development would be summarized as two categories. One of them is the development of chemical pesticides, which include the molecular structure modification of established pesticides for increased safety and synthesis of new safe chemicals which can attack the vulnerable point of physio-ecological characteristics in insect pests and diseases. The other is the biological pesticides which comprise natural enemies and microorganisms to act selectively on confined insect pests and diseases, In addition, improvement of physico-chemical properties of available pesticide formulations would be one of the desirable means for safe pesticide development in view of efficacy enhancement and minimization of hazardous properties or safe pesticide development, various approaches are feasible and needed to study, however, long period and much financial outlay are necessary to develop a new item. And under the present situation in Korea, there are many difficulties for performing research on all the possible routes. Therefore, combined pesticides by the reasonable combination of already registered resticides evaluated as the fairly safe pesticides and safe formulation based on their physico-chemical properties would be developed primarily. And many efforts would be given gradually for the development of new chemical and biological pesticides.

이 시험은 도입남양재 원목에 부착 또는 서식하는 유해병해충의 방제를 목적으로 실시하는 해수수침소독에 있어 농약의 소요량기준을 설정함을 목표로 실시한 것으로써 결과를 요약하면 다음과 같다. 1. 우리나라에 도입되는 남양재 원목의 평균 길이는 11.12m이었으며 직경은 69.55cm이었다. 2. 원목의 전재적에 대한 부상재적은 평균 이었다. 3. 원목의 전재적에 대한 부상표면적의 비의 값은 2.3356( 신뢰수준)이었다. 4. 원목의 전재종 대 부상표면적에 대한 표본추출오차는 1본선내 원목간의 오차(0.2087)가 10본선간의 오차(0.2952)보다 적었다. 5. 원목 당 농약의 적정소요량은 수동식살포기를 이용하는 경우 0.368cc, 그리고 동력분무기를 이용하는 경우는 0.295cc이었다. 6. 예를 들어 남양재 원목 을 도입하여 동력분무기로 해수수침소독을 실시하는 경우 농약소요량은 다음과 같이 계산된다. .

산지별로 채취된 쌀, 보리, 밀에 함유된 잔류농약(유기수은제 및 유기감소제)을 Dithizone법과 GLC법으로 비교분석한 바 이에서 얻은 결과를 요약하면 다음과 같다. 1. Recovery는 유기수은에 있어서 TLC법.에 비하여 GLC법이 양호했고, 유기감소제는 GLC법으로 평균 약 였다. 2. 시료중 유기수은의 잔류수준은 Dithizone법으로 쌀에 있어서 ND-0.053, .보리는 ND-0.051, 밀은 ND-0.033pm이었고, GLC법으로는 쌀은 trace(less than 0.002ppm)-0.036, 보리는 trace-0.030, 밀은 trace-0.025ppm으로 GLC법이 다소 낮은 수치였다. 3. 유기감소제의 잔류수준은 GLC법으로, 쌀에 있어서 BHC(trace-0.0090ppm), DDT(ND-0.0001ppm), Aldrin(trace-0.0032ppm), Heptachlor(race-0.0031ppm), Dieldrin(ND-0.0092ppm), Endrin(ND-0.0057ppm)이었는데, 수은화합물과 모두 공인최대허용량에 월등히 미달되는 안전수준이었다. 4. Column packing은 유기수은분리에는 -DEGS/chromosorb-W,A,W., 유기감소제에는 -DC-200/chromosorb-W,A.W.,가 적당하였는데, 유기수은제의 injection sample의 조제에 있어서 ?를 겸한 정제와 reduced glutatione에 의한 유출처리는 ghost peak의 소거와 성분 peak의 완전분리에 효과가 컸으며 검출감도도 높았다.

Korea represents high suicide rate by poisoning. Especially, since a lot of people died by high concentrated paraquat which has extremely high fatality rate, paraquat sales were prohibited in 2012. We investigated to find out how the prohibition of sales affected occurrence of pesticide poisoned patients and what kinds of influences did it have for severity and death rate. By targeting patients who visited emergency room by pesticide poisoning from 2010 to 2014, we researched insecticides poisoned patients and herbicidal poisoned patients grouped into 9 categories. Before and after 2012, We compared A term (2010, 2011) and B term (2013, 2014)’s pesticide occurrence, especially distribution of herbicide occurrence and compared hospitalization rate, hospitalization period, length of stay in ICU (Intensive Care Unit), severity, and death rate. The number of insecticide patients was 219 (37.4%) and the number of herbicide patients was 367 (62.6%) who visited emergency room in five years and the rate was similar every year. In case of paraquat, there was 70.5% decrease by 105 people for A term and 31 people for B term and increase of double for glyphosate, glufosinate, and other herbicides. The death rate was decreased but hospitalization was increased. There weren’t any difference of rate in ICU admission, intubation and ventilator therapy. By prohibition of using paraquat, the death rate by pesticide poisoning was decreased. However, patients who poisoned by other herbicides were increased, so hospitalization rate increased and there weren’t any difference at patient’s severity rates.

The objective of this study is to investigate the effect of pesticide-related accident on prices and sales of eggs and the perception of food safety accidents among consumers. For this, we analyzed the impact of the pesticide incident on consumers’ purchases by separating large discount stores and eco-friendly specialty stores with econometrics methods. In addition, the value changes for each egg certification were analyzed after the accident. Perception of food safety accidents was conducted through a survey to investigate the awareness of the pesticiderelated accidents, changes in purchases, and the causes of the pesticide accidents. Furthermore, the risk analysis was conducted. This results show the importance of trust and communication in food safety accidents between distributors, consumers and concerned authorities. Also, after the accident, consumers’ interest and premium exist in the breeding process such as animal welfare, not only in the final product. Therefore in order to actively respond to food safety accidents such as pesticidesrelated accident, response and improvement are necessary considering various aspects such as risk assessment, risk management, and risk communication.