This study was conducted to secure basic data for developing technologies to reduce the generation of odor substances by investigating the effects of environmental temperature on growth performance and the generation of odor substances from feces in growing pigs. A total of 16 pigs (Landrace × Yorkshire × Duroc, average body weight 56.49±0.47kg) were randomly assigned to two treatments: thermal-neutral (TN) and heat stress (HS) conditions. The experiments were conducted for two weeks, with average temperature-humidity indices of 68.91±0.09 for TN and 85.98±0.08 for HS. The results showed that HS significantly decreased average daily feed intake (ADFI, 33.3%) and average daily gain (ADG, 25.8%) compared with TN (p<0.05). Non-esterified fatty acid in serum was lower (36.2%) in HS compared with TN (p<0.05). However, protein, blood urea nitrogen, cholesterol, triglyceride, glucose, and IgG in serum showed no difference between HS and TN. Phenol (350.0%) and skatole (416.3%) were significantly higher in HS than in TN (p<0.05). The decrease in growth performance is attributed to reduction in ADFI. The increase in phenol and skatole in HS is presumed to be due to the effect of HS on the metabolism of intestinal microbial composition. Digestion rate, intestinal microbial composition, and urine emissions are known to affect odor substances. Further research on the content of odor substances in urine, nutrient digestion rate, and intestinal microbial composition is considered necessary to determine the exact associations.

γ-aminobutyric acid (GABA) is a natural stress-reducing substance and is actively used as a human health supplement. However, minimal research has been conducted on its use in cattle. This study was conducted to evaluate the effects of GABA on the growth performance, complete blood count, blood metabolites, and carcass characteristics of fattening Hanwoo steers. Twenty-one fattening steers were randomly assigned to one of three treatments: control (basal diet: concentrate feed + rice straw), T1 (basal diet + GABA supplement at 0.8% of feed/head/day), and T2 (basal diet + GABA supplement at 1.6% of feed/head/day). Feed intake and conversion ratio did not differ among the treatments. However, the average daily gain during the early fattening period increased linearly to 0.80 kg, 0.86 kg, and 0.92 kg in the control, T1, and T2 groups (p<0.05). Plasma γ-glutamyl transferase (37.5, 58.2, 42.8 U/ℓ) and creatinine (1.32, 1.34, 1.49 ㎎/㎗) levels in the GABA supplement group were increased compared to those in the control group. The carcass weight (422.7, 427.1, 454.1 kg), rib eye area (86.67, 92.57, 91.14 cm2), and marbling score (3.67, 4.29, 4.14) were numerically higher in the GABA supplement group than in the control group. Thus, GABA supplementation positively affected the average daily gain and carcass characteristics of Hanwoo steers. However, the effect of the GABA supplement level of GABA was small, and further research using rumen-protected coating technology on GABA is necessary.

돼지의 체세포 핵이식(Somatic cell nuclear transfer,SCNT)은 인간에게 약리적 효과가 있는 단백질, 이종 간 장기이식(xenotransplantation)에 사용되는 장기, 질병 연 구 목적의 모델 동물을 제공한다. 특히 형질전환 돼지를 활용한 심장 이식이 세계 최초로 성공한 후 형질전환 돼 지 생산의 안정화는 다음 연구를 위한 중요한 점으로 대 두되고 있으나, 미니돼지의 체세포 핵이식 배아의 생산 효율은 아직 낮은 실정이다. 형질전환의 성공은 양질의 SCNT 배아 생산에서 시작되어야 한다. 이러한 SCNT 배 아의 생산 효율을 향상할 수 있는 요인 중에는 공여 세포 의 형태가 있으며, 성공적인 공여 세포의 생산을 위해서 는 종축에 따른 세포의 특성을 파악하여야 하고, 혈액형 의 차이에서 발생하는 문제점 해결을 위해 OO 타입의 선 별이 필요하다. 본 연구에서는 지속적인 계대 배양을 통 하여 공여 세포로 사용되는 미니돼지의 태아섬유아세포의 계대 배양 조건을 확립하고자 한다. 또한 미니돼지의 혈 액형을 PCR 기반으로 분석하여 분류하고 OO 타입의 선 별을 통하여 이종 간 이식에 용이하게 공여 세포의 조건 을 확립하였다. 이후 sgRNA(single guide RNA)를 사용하 여 CRISPR-Cpf1로 GGTA1(α-1,3 galactosyl-transferase) 유전자를 knock-out 한 미니돼지의 생산으로, 급성면역반 응을 유발하는 Gal(1,3)Gal epitope이 제거된 미니돼지의 세포 주를 구축 및 체세포 핵이식을 통해 GGTA1 knock-out 미니돼지를 생산하였으며, 이러한 연구는 이후 체세포 핵이식 및 이종 간 장기이식에 중요한 기초자료로 사용될 것이라고 생각된다.

This experiment was conducted to investigate the effects of feeding TMR(Total Mixed Ration) with Sasa quelpaertensis Nakai of Jeju cross-bred horses on the body weight and blood composition. Fourteen herds of Jeju cross-bred horses older than 36 months were selected as experimental animals. The experiment was conducted by dividing the herds into seven herds for feeding TMR with 20% Sasa quelpaertensis Nakai(treatment) and another seven herds for feeding TMR without Sasa quelpaertensis Nakai(control) and water were fed ad libitum. In the 12th week, the MPV(mean platelet volume) was statistically significantly higher with Sasa TMR than with control (p<0.05). However, both MPV levels are within a normal range and there were no health problems. With regard to the levels of cholesterol, there was a statistically significant difference between the 33.8±5.9 ㎎/㎗ with individual management and 25.4±8.2 ㎎/ ㎗ with control group (p<0.05). But It are also within a normal range and there were no health problems. In conclusion, feeding TMR with 20% Sasa quelpaertensis Nakai of Jeju cross-bred horses could be utilized as a feeding method for horse.

The present study was conducted to investigate the effect of environmental temperature and backfat thickness (BT) on the reproductive performance of lactating sows. Sixty crossbred sows were allotted to four groups in a 2×2 factorial arrangement by environmental temperature (high temperature [HT], 27.5±1.76℃; low temperature [LT], 23.3±0.89℃) and BT (<20 mm, average 17.70 mm; ≥20 mm, average 23.20 mm) from July to August 2019. Sows in the HT group experienced a greater change in BT and a lower feed intake. Losses in body weight and BT were lower in sows with <20 mm BT than in those with ≥20 mm BT. Sows with low BT had a lower weaning-to-estrus interval than sows with high BT (5.20, 4.93 d vs. 5.87, 5.60 d, respectively). Piglet survivability was lower in the HT group (90.31%) than in the LT group (94.87%). Piglet weaning weight and average daily weight gain were greater in sows with <20 mm BT (5.75 kg and 201.46 g, respectively) than in those with ≥20 mm BT (5.49 kg and 188.41 g, respectively). Sows in the HT group had higher cortisol concentrations than those in the LT group (post-farrowing: HT 7.86 μg/mL vs. LT 6.04 μg/mL; weanling: HT 5.48 μg/mL vs. LT 4.40 μg/mL). In conclusion, environmental temperature adversely influenced sow performance and cortisol levels. Moreover, sows with low BT had a greater weaning-to-estrus interval when subjected to heat stress.

본 연구는 grooming brush의 제공에 따른 한우 암소 육성우의 사양성적, 행동특성, 혈액 내 cortisol 및 혈액성상의 변화를 조사하였다. 6개월령의 암소 육성우(110.63±12.23kg) 32두를 32m2 우방에서 대조 구(non grooming brush), 처리구 1(Chevron angle type grooming brush, CHAT), 처리구 2(Straight type grooming brush, ST) 및 처리구 3(Chevron angle type and Straight type grooming brush, CHAST)으로 처리당 4두씩, 4처리 2반복으로 완전임의 배치하여, 2주간 예비시험 후 8개월간 본 시험 을 실시하였다. 시험결과 grooming brush 제공에 따른 사양성적으로 종료 시 체중과 일당증체량에서 는 대조구 대비 처리구에서 유의적(p<0.05)으로 증가하였고, 사료요구율에서는 대조구에서 가장 낮은 사양성적을 보였다(p<0.05). 행동특성 중 횡화 및 걷기시간은 대조구 대비 grooming brush를 제공한 모든 처리구에 유의적으로 높게 나타났다(p<0.05). 음수행동의 빈도는 처리구 3에서 유의적(p<0.05)으 로 증가하였다. 또한, 몸단장행동인 자기몸 핥기, brushing 행동과 투쟁행동은 대조구에서 다른 처리구 에 비해 유의적으로 증가하였다(p<0.05). 혈액성상에서 헤모글로빈수치는 처리구에서 유의적(p<0.05) 으로 높았고, 스트레스 호르몬인 cortisol 농도는 대조구에서 유의적(p<0.05)으로 높은 수치를 보였다. 따라서, grooming brush 제공에 따른 한우 육성 성적, 행동특성 그리고 혈액 내 cortisol의 변화는 집 단생활을 하는 소의 사회적인 행동에 긍정적인 영향을 미치는 것으로 볼 수 있으며, 스트레스 저감으로 인해 건강한 발육에 기여하는 것으로 나타났다.

본 연구는 국내 실정에 맞는 적정 TDN/CP 비율을 결정하기 위하여 4.6:1(T1)과 4.3:1(T2)의 비율로 생후 13개월령 육성우에게 급여하여 영양소 섭취량, 소화율, 반추 위액 특성 및 혈액조성에 대하여 조 사하였다. 건물 섭취량은 9.0kg/day로 동일하였으며, 조단백질 섭취량은 T1이 1.34kg/day, T2가 1.45kg/day로 T2가 조금 높았으나 유의적인 차이는 없었다. 섬유소 섭취량과 소화율은 T1이 조금 높은 경향을 보였으나 유의적인 차이는 없었다. 사료 섭취 후 4시간째 위액의 pH, ammonia-N, VFA 농도 를 비교한 결과 처리구간 유의적인 차이를 나타내지 않았다. 전반적인 VFA 수치는 TDN/CP 비율에 따 라 큰 영향이 없었으나 T1 처리구에서 iso-butyrate, iso-valerate 및 A/P ratio가 유의적으로 높은 결과를 보였다(p<0.05). 혈액 특성에는 처리구간 유의적인 차이를 나타내지 않았다. TDN/CP 비율이 4.3:1과 4.6:1 수준은 반추위 특성에 큰 영향을 미치지 않았지만, 소화율과 비교하였을 때 생후 13개월 령 육성우 시기에는 섬유소 섭취량과 소화율이 증가되는 4.6:1 수준이 보다 적합할 것으로 판단된다.

농장동물복지형 한우 사양관리를 통한 고품질의 쇠고기 생산을 목표로 세계적인 트랜드에 부합하는 축산선진국으로 나아가기 위하여 본 시험으로 우방내 사육공간의 넓이차이에 따른 한우의 사양성적, 행 동특성 및 혈액성상에 미치는 영향을 비교·분석해 보았다. 16개월령의 한우 54두(암소 미경산공태우 및 거세우 각각 27두)를 공시하여 성별에 따라 사육공간의 넓이차이를 주기 위해 4두(8m2), 3두 (10.67m2) 및 2두(16m2)로 처리하여 9개월간 각각 3반복으로 완전 임의배치 후 실시하였으며, 한우 사 양관리 프로그램에 따라 사료급여를 하였다. 데이터 수집방법으로 증체량, 사료요구율 및 혈액분석은 2 개월 간격으로 반복하여 측정하였으며, 행동패턴 수집은 복수개체 추적방법 및 선택적 행동관찰법으로 기상청에서 제공한 맑은 날을 선정하여 15일간 녹화 후 분석에 이용하였다. 본 시험의 결과로, 사양성 적(체중, 일당증체량 및 사료요구율)의 경우 미경산공태우와 거세우 각각 20개월령과 18개월령 이후부 터 2두와 3두 처리구에서 모두 유의적(p<0.05)으로 향상되었다. 행동특성으로는 미경산공태우의 2두 처 리구에서 행동시간으로 기립행동은 유의적(p<0.05)으로 낮고, 횡와 및 걷기행동은 유의적(p<0.05)으로 높았다. 행동빈도에서는 음수, 자기몸핥기, 긁기, 몸비비기, 투쟁행동에서 유의적(p<0.05)으로 적게 나 타났다. 거세우의 2두 및 3두 처리구에서는 미경산공태우와 유사한 경향을 나타냈으나 유의적(p<0.05) 으로 섭식시간은 증가하고, 다른소핥기 및 몸기대기의 빈도는 유의적(p<0.05)으로 적게 나타났다. 혈액 성상으로 미경산공태우와 거세우의 4두 처리구에서 적혈구가 유의적(p<0.05)으로 낮았고, 콜티졸은 유 의적(p<0.05)으로 높게 나타났다. 또한, 미경산공태우의 헤모글로빈과 거세우의 백혈구 수치에서는 모 두 2두 처리구에서 유의적(p<0.05)으로 각각 높고 낮은 것으로 분석되었다. 이상의 결과를 종합하면 사 양성적, 행동특성 및 혈액성상 등의 전체적인 내용을 토대로 우사의 여유 및 표준우사의 우방크기를 고 려할 때 한우의 사육공간에서 관행사육인 4두 처리구(두당 8m2) 보다 미경산공태우는 2두 처리구(두당 16m2), 거세우는 3두 처리구(두당 10.67m2)로 사육하는 것이 적절하다고 판단되며, 본 시험결과는 추후 복지형 한우의 사양관리를 위한 기초자료로 활용할 수 있을 것으로 판단된다.

The aim of this study was to investigate the effects of different rearing spaces on growth performances, behavioral traits, and blood parameters in Hanwoo (Korean Native Cattle) heifers and steers. The average daily gain and feed conversion ratio were measured to assess the growth performance. A total of 54 Hanwoo heifers and steers at 18 months old, were housed separately in groups of 2, 3 or 4 heads in a pen (32 m2) for nine months. In the case of the heifers, the growth performances were significantly (p<0.05) improved by increasing the 2 head group size with the exception of the 18 month old heifers. However, among the steers the growth performances were significantly enhanced (p<0.05) after 18 months of age, by increasing the 3 heads group size. In addition, the heifers in groups of 2 heads spent less time standing and more time lying down or walking, and also had a significantly (p<0.05) lower frequency of self-grooming, scratching, and fighting. These findings are similar to the results obtained for steers housed in 2 or 3 heads per group. The steers housed in 2, 3 heads groups spent more time feeding and consequently had a significantly (p<0.05) lower frequency of pair-wise grooming and Leaning. The heifers and steers housed in groups of 4 heads showed lowest red blood cell counts, but highest cortisol content with statistical significance at p<0.05. The heifers in groups of 2 heads had the highest hemoglobin concentrations (p<0.05). On the other hand, white blood cell count was found to be the lowest in groups of 2 steers with statistical significance at p<0.05. We conclude that a large rearing space improved growth performances, behavioral characteristics, and blood parameters in Hanwoo heifers and steers. In the future, this data could be used to improve the welfare of rearing Hanwoo cattle.

The objective of this study was to monitor health conditions of genetically identical somatic cells cloned Korean white cattle, endangered indigenous cattle (EIC) and indigenous cattle (IC) by analysis of hematologic characteristics. Naturally ovulated oocytes and donor cells were used for somatic cell nuclear transfer (SCNT). Donor cells and enucleated oocytes were followed by electric fusion, chemical activation and surgical embryo transfer into the oviducts of surrogate females. Two recipients became pregnant; two maintained pregnancy to term, and one live cattle were delivered by caesarean section. The cloned Korean white cattle were genetically identical to the nuclear donor cattle. As a result, the mean values of RBC and platelet of cloned cattle and white cattle were significantly decreased by age (P<0.05). The mean values of RBC, HCT, MCV and MCHC between cloned cattle and IC of the same age (1∼2 years) showed the statistical significance (P<0.05). Also, in the WBC of Korean white cattle, the estimated values were decreased according to the age from 12.0×103/μl under 1 year to 11.0×103/μl over 1 years respectively. Although clone-cattle had lower numbers of RBC than reference range, the most of RBC and WBC related heamatologic results of cloned cattle were not different when compared to reference range. This study suggests that cloned Korean white cattle derived from SCNT did not have remarkable health problems, at least in the growth pattern and hematological parameters. In addition, this study provides a valuable resource for further investigations of the preservation of rare genetic stocks underlying traits of interest in cattle.

본 연구에서는 백혈구 친화 흡착 및 분리 가능한 기능성 필터를 개발하기 위해, 직경이 3μm이하를 가지는 부직포 표면에 전기 방사법을 이용하여 평막형태의 혈액분리용 필터를 제조하였다. 전기방사는 고분자 농도, 유량, 인가전압, 에어갭 등의 변수를 조절하여 나노/마이크로 구조를 최적화하였다. 분리막의 단면과 표면 모폴로지는 전계방출형주사현미경(FE-SEM)을 이용하여 관찰하였다. 화학적 표면처리를 통해 친수성을 증가시켰으며, 혈액적합성 평가결과 우수한 것을 확인 할 수 있었다.

The importance of genetic resource preservation has been highlighted in the literature as a means of maintaining genetic diversity. Investigations for hematologic values and the differential count of white blood cell count (WBC) for Korean indigenous cattle (KIC) and endangered indigenous cattle (EIC) are rarely performed. Therefore, the objective of this study was to investigate the hematologic values of total 40 EIC (White, Black, Mini cattle) and 35 KIC as control by analysis of hematologic characteristics. As a result, the mean values of RBC and platelet of EIC were significantly decreased by age (p<0.05). The mean values of RBC, HCT, MCV and MCHC between EIC and KIC of the same age (2 ～3 years) showed the statistical significance (p<0.05). Also, in the WBC of EIC, the mean values were decreased according to the age from 13.9×103/μL～12.7×103/μL under 1 year to 9.1×103/μL～11.5×103/μL over 2 years respectively. In the differential count of WBC of EIC (White, Black, Mini cattle), it showed generally the rates of 40.2%, 52.2%, 49.0% lymphocyte and 27.2%, 33.9%, 32.0% segmented neutrophil from 2～3 years respectively. Result of this study will be used for establishing reference range for blood analysis in EIC such as white, black and mini cattle. This study reported hematological values which could serve as baseline information for comparison in conditions of nutrient deficiency, physiological and health status of endangered Korean native cattle. In addition, this study provides a valuable resource for further investigations of the preservation of rare genetic stocks underlying traits of interest in cattle.

본 연구는 다양한 수준의 셀레늄 강화 청보리 사일리지 와 무기셀레늄을 급여 시 비육기 거세한우의 생산성, 혈액 성상, 도체특성 및 조직 내 셀레늄 축적에 미치는 영향을 조사하였다. 시험은 거세비육우 20두를 공시하였고, 처리구 는 셀레늄 수준에 따라 0.1 ppm Se(대조구), 0.3 ppm Se (T1), 0.9 ppm Se (T2) 및 무기셀레늄 0.9 ppm Se (T3) 급 여구로 4처리를 나누어 처리구 구당 5두씩 완전임의 배치 하여 90일간 사양시험을 실시하였다. 각 시험사료는 셀레 늄 강화 및 셀레늄 무처리 관행 청보리 사일리지를 조합하 여 건물기준 30% 함유하도록 배합하였다. 실험사료 내에 셀레늄 강화수준은 사료섭취량과 증체에 영향을 미치지 않 았고, 혈중 총 지질과 중성지방농도는 대조구보다 T2구가 유의하게 낮았다(p<0.05). 혈중 면역글로불린은 셀레늄함유 량이 많을수록 높게 나타나 T2와 T3구가 대조구보다 유의 하게 높았다(p<0.05). 혈중 셀레늄농도는 유기태 셀레늄을 0.9 ppm 급여한 T2구가 가장 높았다(p<0.05). 도체율, 배최 장근, 등지방두께, 근내지방도, 육색 및 지방색은 셀레늄급 여로 인한 효과가 나타나지 않았다. 또한, 사료 내 셀레늄 수준 및 형태는 육질 및 육량등급 간에 관련성이 형성되지 않았다. 신장, 간장 및 후지 내 셀레늄 함량은 사료 내 셀 레늄 강화수준이 증가함에 따라 유의하게 직선적으로 증가 하였고(p<0.05), 무기셀레늄급여는 대조구와 유의한 차이가 나타나지 않아, 조직 내 셀레늄함량 증가에 도움이 되지 않았다(p>0.05). 이상의 결과에서 셀레늄 강화 청보리 사일 리지 내 존재하는 셀레늄은 조직 내 셀레늄함량을 증가시 키기에 유효한 셀레늄급원인 것으로 나타났고, 셀레늄 강 화 청보리 최고수준(0.9 ppm Se)으로 급여하여 생산된 한 우 후지육 100 g에 함유되어 있는 셀레늄은 성인기준 1일 최소 셀레늄 권장량(40 ㎍)의 약 69%를 충족시키는 것으로 조사되었다.

This study was conducted to investigate the effect of enzyme and yeast extract supplement on growth performance, carcass characteristics and blood parameters in Hanwoo steers. A total of forty-eight 23-month-old Hanwoo steers, with average initial body weight of 545.25±12.60 kg, were assigned to four treatments with three replicates in a completely randomized design. Hanwoo steers housed in four heads group per pen. The treatments comprised of four groups of control (no feed additives), treatment 1 (xylanase, cellulase, and gluco-amylase; 0.1%/concentrate), treatment 2 (β-glucan and mannan-oligosaccharide; 0.1%/concentrate), and treatment 3 (treatment 1+2; 0.1%/concentrate). During the fourth and eighth month, average daily gain and feed conversion rates improved in treatment 1, but in the control this was lower than the other treatments (p<0.05). Real-time ultrasound was used for measuring the live carcass traits (longissimus muscle area, back fat thickness, and marbling score) and the better results were in the following order: treatment 1 > treatment 3 > treatment 2 > control, and extended to carcass grade. White blood cell indices were lower in treatments 2 and 3 and the IgG indices were relatively higher than in other treatments. As a result, levels of xylanase, cellulase, and gluco-amylase were increased from the fourth month to improve growth performance, live carcass traits. Durin

The objective of this study was to monitor health conditions of four genetically identical somatic cells cloned Labrador retriever puppies by estimation of body weight and analysis of hematologic and serologic characteristics. Naturally ovulated oocytes and donor cells were used for somatic cell nuclear transfer (SCNT). Donor cells and enucleated oocytes were followed by electric fusion, chemical activation and surgical embryo transfer into the oviducts of surrogate females. Two recipients became pregnant; two maintained pregnancy to term, and four live puppies were delivered by Caesarean section. The cloned Labrador retrievers were genetically identical to the nuclear donor dog. The body weight of clone-1, -2, -3, and -4 was increased from 0.66, 0.40, 0.39, and 0.37 kg at birth to 6.2, 6.6, 6.2, and 6.0 kg at 8 weeks of age, respectively. Although clone-4 had lower numbers of RBC than reference range, the most of RBC and WBC related heamatologic results of cloned puppies were not different when compared to reference range. In serological analysis, Glucose, ALP and inorganic phosphate level of four cloned puppies was significantly higher than the reference ranges. However, there was no significant difference among four cloned dogs. This study suggests that cloned puppies derived from SCNT did not have remarkable health problems, at least in the growth pattern and hematological and serological parameters.

본 연구는 돼지의 육성기 또는 비육기에 Bio 이온수 급여에 따른 성장, 혈액분석 및 육질 특성 평가를 위하여 실시하였다. 시험구는 Bio 이온수 무 급여구 (대조구), 육성기 급여구 및 비육기 급여구로 3시험 구를 두었으며, 각 시험구 당 33두의 3원교잡종 (Landrace☓Yorkshire☓Duroc) 돼지를 배치하였으며, 총 99두를 이용하여 사양시험을 수행하였다. Bio 이온수 급여는 육성돈과 비육돈의 성장과 사료효율에 영 향을 미치지 않았지만 (P>0.05), 비육기 급여구에서 일당증체량과 A등급 출현율이 높게 나타났다. 대조구 에 비해 Bio 이온수를 급여한 처리구에서 혈액성상 분석 결과 적혈구와 백혈구의 수치가 증가하였다 (p<0.05). 일반성분, 육색, pH, 육즙감량, 가열감량 및 전단력에서 유의적인 차이는 나타나지 않았다. 지방 산 분석 결과 육성기 급여구에서 포화지방산/불포화지방산 비율이 낮게 나타났고, 불포화지방산의 함량 비 율이 가장 높게 나타났다. 또한 가열육 관능검사에서 향과 전체적인 기호도에서 높은 점수를 획득하였다.

This study was performed to examine the effects of recombinant bovine somatotropin (rBST) and fermented alcoholic feedstuff(FAF) on blood metabolites, carcass characteristics and profitability. Sixteen Hanwoos(bulls; 8, steers; 8) with average 143㎏ of body weight were randomly assigned to bull control group(BC), steer control group(SC), bull group(BrA) treated with rBST and FAF, and steer group(SrA) treated with rBST and FAF. Sustained release rBST(0.03㎎/㎏/body weight) was administered biweekly and dose of rBST was adjusted monthly according to body weight increase. The blood glucose content was higher in the groups treated with rBST and FAF than control groups. The blood cholesterol contents tended to be lower in the treatment groups (BrA, 120.5 mg/dl; SrA, 104.0 mg/dl) than in the control groups (BC, 141.0 mg/dl; SC, 125.5 mg/dl). No significant difference between the groups of bulls and steers were found in the blood triglyceride concentrations as well as the albumin contents. The rib eye area of BC and BrA were 70 and 72 cm2, respectively, which are slightly larger than the area of SC and SrA. The treatment did not affect the backfat thickness both in bulls and steers. The marbling scores of BC and BrA appeared as No. 1 and No. 2, respectively, and No. 3 and No. 4 were obtained in SC and SrA. Lower non saturate fat concentrations were observed in the treatment groups (BrA, 64.30%; SrA, 59.59) than in control groups (BC, 58.98%; SC, 58.09%). Saturated fatty acids and amino acids contents were slightly more improved in the treatment than in the control. Average auction prices of BC and BrA were 7,900 and 8,089 wons per 1kg of carcass, respectively, whereas the prices of SC and SrA were 9407 and 9866 wons per 1kg of carcass, respectively. It is concluded that the treatment with rBST and FAF can improve the growth performance such as marbling scores and rib eye area and increase the auction prices.