This study was aimed at evaluating the antifungal potentials of twelve plant extracts (Terminariae Fructus, Glycyrrhizae Radix, Cinnamomi Cortex, Taraxacum Platycarpum, Rubi Fructus, Caesalpiniae Lignum, Myristicae Semen, Caryophylli Flos, Sanguisorbae Radix, Alpiniae Katsumadaii Semen, Scutellariae Radix, Astragalus membranaceus) in preventing storage rot symptoms in ginger (Zingiber officinale). Samples were collected from ginger that had been stored for 120 days at 10-12 . A total of ten fungi were isolated from stored non-spoiled and spoiled ginger and identified using internal transcribed spacer gene sequencing as Hypocrea virescentiflava, Fusarium oxysporum, Acremonium strictum, Fusarium solani, and Cadophora fastigiata. The causal fungus of storage rot was identified as F. oxysporum by pathogenicity test. Twelve candidate plant extracts were screened for antifungal activity against F. oxysporum isolated from spoiled ginger by using the disk diffusion method. In minimal inhibitory concentration (MICs) test, all ethanolic extracts of Cinnamomi Cortex, Taraxacum platycarpum, and Caryophylli Flos exhibited higher antifungal activity against F. oxysporum than other plant extracts. The MIC value of Cinnamomi Cortex and Caryophylli Flos were determined to be 10 ìg/disc. The MIC of T. platycarpum, 2.5 ìg/disc, was the lowest. Based on the results, it was concluded that Cinnamomi Cortex, T. platycarpum, and Caryophylli Flos have potential to serve as effective treatments to prevent storage rot of ginger.

Propionibacterium acnes (P. acnes)는 여드름의 주요 병원성균으로 염증성 질환에 관련된 미생물 중 에 하나이다. 본 연구에서는 여드름 주요 유발 균주인 P. acnes에 대하여 70% Ethanol로 추출한 제주 자생 식물 추출물의 항균활성을 disc diffusion test 실험을 이용하여 측정하였다. 실험 결과 61개의 제주 자생 식물 추출물 중에서 45개 추출물의 항균 활성이 검출되었으며, 16개의 추출물은 항균 활성이 검출되지 않았다. 그중 에서 여우 구슬(Phyllanthus urinaria L.) - 줄기⋅잎 추출물이 18.96 ± 0.69 mm로 가장 높은 항균 활성을 나타내었고, 참당귀(Angelica gigas Nakai) - 뿌리, 돌외(Gynostemma pentaphyllum (Thunb.) Makino), 참당귀(Angelica gigas Nakai) - 잎⋅줄기, 산박하(Isodon inflexus (Thunb.) Kudo), 차나무(Camellia sinensis L.) - 꽃, 그리고 로즈마리(Rosmarinus officinalis L.) - 줄기 추출물 순으로 높은 항균 활성을 나타내었다. 이러한 결과는 제주 자생 식물 추출물이 여드름 치료⋅예방의 항균제 및 기능성 화장품 천연 원료로써의 이용 가능성을 제안한다.

Background: Allergic diseases like such as atopic dermatitis, asthma, and rhinitis have recently increased both domestically and globally. The present study was undertaken to select candidates with anti-allergic activity from plant resources. Methods and Results: Fifty-six plant extracts at 20㎍/㎖ were screened against β-hexosaminidase production and interleukin (IL)- 4 release in degranulated rat basophilic leukemia (RBL)-2H3 cells. The anti-allergy activity of three plant extracts selected from the preliminary screening experiment, Polygonatum sibiricum F. Delaroche (root), Pyrus pytifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud (root) were measured at concentrations of 2 - 250㎍/㎖ in three cell lines as RBL- 2H3, HaCaT and Jurkcat T cells. The assay showed the root extract of R. glutinosa to have an inhibitory activity of 4.2% - 28.6% on β-hexosaminidase production from IgE-sensitized RBL-2H3 cells. Each extract of P. sibiricum and R. glutinosa reduced IL-4 release in IgE-sensitized RBL-2H3 cells, respectively. The leaf extract of P. pyrifolia var. culta showed a significantly potent suppressive effect of 10.2% - 74.7% on the mRNA expression of tumor necrosis factor (TNF)-α in HaCaT cells sensitized with TNF-a and INF-g, and showed inhibitory effect of –8.6% - 90.9% on the mRNA expression of IL-2 in Jurkat T cells sensitized with PMA and A23187. Conclusions: The results showed that the root of R. glutinosa and leaf of P. pyrifolia var. culta could be useful candidates as antiallergy materials.

This study was conducted to evaluate natural plant extracts for methane gas reduction in ruminants. Rumen fluid was collected from cannulated Hanwoo cow (450±30 kg) consuming 400 g/kg concentrate and 600 g/kg timothy. The 15 ml of mixture comparing McDougall’s buffer and rumen fluid in the ratio 2 to 1, was dispensed anaerobically into 50 ml serum bottles. Rumen fluid contents were collected and in vitro fermentation prepared control (timothy, 300 mg), ginseng, balloon flower, yucca plant, camellia, tea plant and ogapi extracts were added at the level of 5% against 300 mg of timothy as a substrate (v/w) and incubated for 3, 6, 9, 12, 24, 48, and 72 h. In vitro pH values range 6.55~7.41, this range include rumen titration. The dry matter digestibility was not differ between all treatments and control. Total gas emission was significantly higher (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Carbon dioxide emission was not differ all treatments on 9 h than in control and significantly higher (p<0.05) yucca plant, camellia and tea plant treatments on 12 h than control. Methane emission was not differ all treatments on 6 h than in control. The rumen microbial growth rate was significantly higher (p<0.05) in ginseng, balloon flower on 12 h and significantly higher (p<0.05) in ginseng, yucca plant, tea plant and ogapi treatments on 24 h than in control. Total VFA was significantly higher (p<0.05) in tea plant and ogapi treatments on 12 h than in control and significantly higher (p<0.05) in ginseng, balloon flower treatments on 48 h than in control. Acetic acid was significantly lower (p<0.05) in ginseng and balloon flower treatments on 24 h than in control. Propionic acid was significantly higher (p<0.05) in ginseng and balloon flower treatments on 48 h than in control. As a results, sixth natural plant extracts had no significant effect dry matter digestibility and negative on rumen fermentation, but not effect methane reduction.

Plant extracts were screened for antifungal activity against major plant pathogens, Botrytis sp., Collectotrichum sp., Alternaria sp. and Cylindrocarpon sp. using 96-well microdilution method. Among the 662 methanol extracts from 401 plant species, 36 extracts showed complete inhibition of spore germination against at least one of four pathogenic fungi. Extracts of Morus alba twig and Sophora flavescens root showed minimum inhibition concentration (MIC) at 1,250 ㎍/㎖ against Botrytis sp.. Extracts of Chloranthus japonicus root showed MIC at 1,250 ㎍/㎖ against Collectotrichum sp.. Extracts of Glycyrrhiza uralensis aerial part, Inula helenium root and Menispermum dauricum root showed MIC between 625 and 1,250 ㎍/㎖ against Alternaria sp.. G. uralensis aerial part and I. helenium root showed MIC at 1,250 ㎍/㎖ against Cylindrocarpon sp.. Specifically, the extracts of Agrimonia pilosa root, Angelica tenuissima root, Asarum sieboldii root, Campsis grandifolia leaf and twig, Cnidium officinale root, Dictamnus dasycarpus root, G. uralensis aerial part, I. helenium root and M. alba twig completely inhibited spore germination at lower than 5,000 ㎍/㎖ against all of four pathogenic fungi. Two methanol extracts from G. uralensis aerial part and M. alba twig may used as a candidate to develop into effective disease management materials in plant cultivation.

본 연구에서는 효과적이고 안전한 화장품 성분 탐색을 위해 항산화 활성 가이드라인에 따라 다양한 식물 추출물을 대상으로 효능을 확인하였으며 이 중 가장 효능이 좋은 식물을 발굴하고 이를 최적의 비율로 혼합하여 Charmzone Extract (CZE)로 명명하였다. CZE는 하엽(Nelumbo nucifera leaves), 삼백초(Saururus chinensis) 및 와송(Orostachys japonica)으로 구성된 식물복합추출물로, 본 연구에서는 CZE의 항노화 화장품 원료로서의 개발 가능성을 확인해 보고자 하였다. CZE는 자유 라디칼 소거 활성을 가지고 있었으며, 인간 각질 형성세포주인 HaCaT 및 인간 섬유아세포 NHDF에서 활성산소종의 생성을 억제하고 총항산화능을 증가시켰다. 그리고 NHDF에서 제1형 프로콜라겐의 분비를 촉진하였고, B16F10 세포에서 멜라닌의 생성을 감소시켰으며, RAW264.7 세포에서 lipopolysaccharide 자극에 의한 산화질소의 생성을 농도 의존적으로 저해하였다. 또한, CZE는 HaCaT의 이동을 촉진하는 효능을 나타내었다. 결과를 종합하면, CZE는 항산화, 주름개선, 미백, 항염증 및 상처치유의 다효능을 나타내며, 이러한 효능을 통해 피부 기능을 향상시킬 수 있을 것이라고 기대된다. 그러 므로 식물복합추출물 CZE는 피부 항노화용 화장품 성분으로 응용 가능하다.

Background : This study was carried out to investigate the cytotoxicity in 9 extracts from 8 medicinal plants, such as leaf extract of Lonicera maackii (Llm), leaf extract of Platycarya strobilacea (Lps), flower extract of Fagopyrum dibortryis (Fdf), stem extract of Physostegia virginiana (Spv), root extract of Allium senescence (Ras), aerial part extract of Allium schoenoprasum (Aas), aerial part extract of Artemisia japonica var. manshurica (Aaj), stem extract of Caryopteris incana (Sci), and leaf extract of Caryopteris incana (Lci), on human cancer cell lines. Methods and Results : Dried plant extracts were granted from National Institute of Horticultural and Herbal Sciences. The extracts of each plant were dissolved in DMSO and stored in deep freeze at –20℃. The cell viabilities were examined by MTT assay. On SK-OV-3 cell line, Lps, Aas, Sci ans Lci showed dose-dependent cytotoxic effect. On A549 cell line, almost samples show dose-dependent cytotoxic effect, but especially Aaj showed relatively high cytotoxic effect. In case of HCT-15 cell line, Llm and Aas showed relatively high cytotoxic effect. Conclusion : These results suggested that Lonicera maackii, Platycarya strobilacea, Fagopyrum dibortryis, Physostegia virginiana, Allium senescence, Allium schoenoprasum, Artemisia japonica var. manshurica, and Caryopteris incana can be utilized as potential sources of anticancer agent due to their cytotoxicity.

The susceptibility of the predatory mite, Neoseiulus californicus to extracts of Melia azedarach, Piper nigrum, Syringa velutina and their mixtures was evaluated in laboratory bioassays. In treatments with plant extracts tested, 76-86% of adult female predators survived after 7 days after treatment. Adult female predators treated with plant extracts tested produced 68.3-81.1% as many eggs as did control females and eclosion of eggs deposited by treated predators was not affected. Moreover, treatment of plant extracts tested showed no toxic effect on N. californicus eggs and produced 100% hatchability. Survival of N. californicus nymphs was not seriously affected by exposure to the plant extracts tested. Immatures of N. californicus survived on the leaf disc treated with plant extracts tested and 78-90% of immature predators reached adulthood. These results suggest that extracts of Melia azedarach, Piper nigrum, Syringa velutina and their mixtures expected to be promising candidates for use in integrated mite management program with N. californicus.

The Wnt/β-catenin signaling pathway is mandatory in adipogenesis. In this study, we investigated the applicability of functional materials for the treatment of obesity by examining Wnt/β-catenin pathway reporter activity related to adipocyte differentiation inhibiting with korean native plant extracts. The luciferase activity of HEK 293-TOP cells increased the reporter activity approximately 152% and 130% by treatment with Sanguisorba officinalis and Thuja orientalis, respectively. Ricinus communis were represented about 90% higher activity, two samples(Rosa rugosa and Sophorae Flos) showed 80% higher activity than the control. Three samples of plant extracts (Zanthoxylum piperitum, Pueraria thunbergiana, Solanum nigrum) were about 70% higher activity compared with the non-treated control. Cytotoxicity of plant extracts was not detected in the rat neural stem cells. These results suggest that the selected eight plant extracts are safe compounds. Our findings indicate that Wnt/β-catenin pathway reporter activity could be used for high throughput screening system. In addition, the plant extracts selected as candidates for adipocyte differentiation inhibiting may be potential therapeutic agents for obesity, it will be exploring the possibility of developing an anti-obesity materials through further experiments with selected plant extracts.

Background : Allergy is a common disease caused by type I hypersensitivity reaction of the immune system. Unfortunately, there is no proper treatment for allergy. Therefore, the discovery of therapeutic drugs for allergy is essential. In this study, the crude extracts of 56 plant parts were screened for anti-allergy effects in RBL-2H3 cells. Methods and Results : IgE-sensitized RBL-2H3 cells were individually treated with 56 extracts of medicinal herbs at the final concentration of 20 ㎍/㎖ and stimulated with the antigen DNP-BSA. β-Hexosaminidase release, interleukin-4 (IL-4) secretion, and cell viability in the sample treated cells were measured. Among the tested samples, extracts from the root of Polygonatum stenophyllum Maxim., aerial part of Acer triflorum Kom., and leaf of Pyrus pyrifolia var. culta (Makino) Nakai showed inhibitory effects on β-hexosaminidase release. The aerial part of Peucedanum japonicum Thunberg and seed of Panax ginseng C.A.Mey. showed suppressive activities on IL-4 secretion. All of the extracts were not cytotoxic at the tested concentration. Conclusion : From the result, six extracts including Polygonatum stenophyllum Maxim. (root) and Acer triflorum Kom. (aerial part) inhibited both β-hexosaminidase release and IL-4 secretion in IgE-sensitized RBL-2H3 cells. The use of these extracts for developing anti-allergy materials is suggested.

Background : As a part of ongoing research to elucidate and characterize anti-inflammatory nutraceuticals, six kinds of plant extracts (aerial part of Nepeta cataria, leaves of Lonicera maackii, leaves of Platycarya strobilacea, flower of Fagopyrum dibotrys, flowers and fruits of Solanum nigrum, stem of Physostegia virginiana) were tested for their ability to suppress inflammation. The anti-inflammatory has been studied in lipopolysaccharide (LPS)-stimulated RAW264.7 cells which cells synthesized nitric oxide (NO) from L-arginine by nitric oxide synthase (NOS). In this study, NO synthesis inhibitory activity of six kinds of plant extracts on LPS-stimulated RAW 264.7 mouse macrophages was evaluated. Methods and Results : Six kinds of plant extracts were parceled out from RDA (Rural Development Administration). RAW 264.7 cells (1.5×105 cells/well) were seeded onto 96-well plates with DMEM media containing 10% FBS and 1% antibiotics. The cells were pretreated with the extracts and LPS-stimulated cells for 24 h. Cellular NO production was stimulated by adding 1 μg/mL of LPS. After incubation, Griess reagent was used to determine NO production. Absorbance was measured at 520 nm by microplate reader. NO synthesis inhibitory activity potential of these extracts was evaluated by assessing NO production by LPS-stimulated RAW 264.7 cells in the presence. As a result, inhibition rate of NO production was about 40% of L. maackii, 33% of F. dibotrys, 23% of P. strobilacea and 17% of P. virginiana. Meanwhile, there was no significant results in aerial part of N. cataria and flowers and fruits of S. nigrum. Conclusion : From the above results, we be able to confirm that leaves of L. maackii and flower of F. dibotrys appeared dose-dependent NO synthesis inhibitory activity and leaves of P. strobilacea appeared NO synthesis inhibitory activity in low-concentration. As screening NO synthesis inhibition of six extracts, they may be a good candidate for delaying the progression of human inflammatory diseases and warrants further studies.

The susceptibility of the twospotted spider mite, Tetranychus urticae and the predatory mite, Phytoseiulus persimilis to extracts of Melia azedarach, Piper nigrum, Syringa velutina and their mixtures was evaluated in laboratory bioassays. Treatments of mixture 1 and 2 were effective against adult females of T. urticae and yielded 73.3 and 70.7% adulticidal activity at 7 days after treatment, respectively. Treatment of mixture 3 revealed 62.7% adulticidal activity. However, M. azedarach, P. nigrum and S. velutina had lower adulticidal activity than the other treatments. Adult females of T. urticae treated with mixture 1 and 2 produced only 11.1-16.7% as many eggs as control females did. All the plant extracts tested were ineffective to against the eggs of T. urticae. Plant extracts tested had little effect on the survival of P. persimilis adult females. Moreover, reproduction of P. persimilis adult females and eclosion of eggs deposited by treated predators were not seriously affected. Treatment of plant extracts tested showed no toxic effect on P. persimilis eggs and produced 100% hatchability. These results suggest that mixture 1 and 2 might be used for the control of T. urticae, and expected to be promising candidates for use in integrated mite management program with P. persimilis.

A total of 708 methanol extracts from 599 species in 126 families(e.g. Aceraceae) of plant resources were screened for inhibition of cosmetics contaminating microbe, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans using 96-well microplate bioassay. Four plant extracts including Celastrus orbiculatus inhibited the growth of Staphylococcus aureus over 90%, 38 extracts including Acer palmatum var. nakaii inhibited the growth of Pseudomonas aeruginosa over 60% and 10 extracts including Ilex crenata var. microphylla inhibited growth of Candida albicans over 60%. Plant extracts showing growth inhibition activity against S. aureus, P. aeruginosa and C. albicans could be used as a reference guide for the future development of natural preservatives for cosmetics.

이 연구의 목적은 전통적으로 사용되고 있는 천연염색의 원료인 천연염료, 즉 천연색소의 열수추출물과 플로랄워터 의 항산화 활성을 확인하는 것이다. 항산화 활성은 총 페놀 함량 분석과 DPPH 라디칼 소거능, ABTS 라디칼 소거능, 단일항산소 억제 효과로 확인하였다. 열수추출물에서 DPPH 라디칼 소거 활성을 나타내는 IC50 값은 0.0085～3.5 mg/mL를 나타내어 일부 색소 추출물의 DPPH 소거 활성이 우수하다는 것을 알 수 있었다. 특히 오배자, 소목 추출물이 8.5 μg/mL, 19.1 μg/mL로 높은 DPPH 라디칼 소거 활성을 나타내었는데, 비교 물질로 주로 사용되는 ascorbic acid의 10.5 μg/mL 만큼 높은 우수한 항산화력을 가지는 것을 확인 하였다. 단일항산소 억제 효과 역시 오배자, 소목 추출물에 서 각각 0.21, 0.12 mg/mL을 나타내어 높은 소거 활성을 띄는 것으로 강한 빛에 의해 야기되는 활성산소의 피해로부 터 생물체를 보호해주는 것을 알 수 있었다. 총 페놀 함량도 열수추출물에서 높은 수준을 나타내었다. 반면, 플로라워 터의 항산화 활성은 열수추출물 대비 미비한 효과를 보였으 나 황련, 황벽천초근의 경우 열수추출물 보다 우수한 결과 를 나타내었다. 플로라워터의 경우 액상시료를 첨가하여 수행되는 실험 방법에 의해 정량적 수치를 명확히 나타내기 어렵기 때문에 연구방법적 측면에서 개선이 필요할 것으로 판단된다. 결론적으로 일부 천연색소 추출물은 생물체의 산화적 스트레스로부터 야기되는 활성산소를 억제하는 중 요한 역할을 하며, 보다 심도 깊은 연구를 진행한다면 새로 운 생물 소재로 활용성이 높을 것으로 사료된다.

항산화 및 항균활성을 가진 천연물을 활용해 합성보존료를 대체하고자 하는 연구가 활발한 가운데 국내 자생 식물 중 135종을 대상으로 DPPH radical 소거활성을 측정하였다. 이중 항산화 활성이 10%대로 낮게 나타난 식물 8종에 대해 항균효과를 조사하였다. 식물 추출물의 DPPH radical 소거활성은 0.41~94.84%로 다양하였으며, 가장 효과가 좋은 식물은 꽃향유와 우엉으로 각각 94.84, 80.55%를 나타내었다. 우엉은 잎의 경우 26.87, 뿌리 19.29%의 활성을 나타내 동일 식물이라도 추출부위에 따라 활성의 차이가 있을 것으로 보인다. 선이질풀과 비쭈기나무는 각각 그람양성균인 B. subtilis, 그람음성균인 E. coli 및 Candida속인 C. albicans에 대해 2.2~10.8 mm 수준의 항균활성을 나타내었다. 각 식물에 대한 다양한 추출법을 적용하여 기능성을 확인하고 이들 항산화성 물질에 대한 추가 연구가 진행되어야 할 것으로 사료된다.

국내 식물자원 및 약용식물들의 식물 생장촉진 효과를 조사하기 위하여 64종 65점의 추출물 시료를 조제하여 벼(Oryza sativa) 유묘 생장에 미치는 영향을 평가하였다. 그 결과, 11종 12점의 쑥(Artemisia princeps) 경엽, 엉겅퀴(Cirsium japonicum var. maackii) 전초, 진달래(Rhododendron mucronulatum) 가지와 잎, 갈대(Phragmites communis) 줄기, 율무(Coix lacrymajobi var. mayuen) 전초, 싸리(Lespedeza bicolor) 가지와 잎, 산수국(Hydrangea serrata f. acuminata) 전초, 속단(Phlomis umbrosa) 전초, 감초(Glycyrrhiza uralensis) 근경, 감초(G. uralensis) 경엽, 당귀(Angelica gigas) 뿌리 및 천궁(Cnidium officinale) 근경 추출물은 벼 유묘 생장을 촉진시키는 것으로 나타났다. 본 결과에 따라 상기 식물 종의 부위들은 식물 생장 촉진용 농가 자가제조 액비 개발을 위한 소재로써 활용가능성이 있는 것으로 판명되었다.

본 연구는 귀화식물인 Solidago altissima, Amaranthus retroflexus, Sida spinosa 등을 이용하여 친환경 농자재로 개발하기 위한 기초자료를 제공하기 위해 수용성 추출액의 농도에 따른 수용체 식물의 발아 및 유식물 생장과 실험 병원균의 생장을 조사하였다. 공여체식물에 따른 수용성 추출액 농도가 증가됨에 따라 대부분 검정식물의 상대발아율은 감소하는 경향을 보이는데 S. spinosa(r = -0.540, p＜0.01), Physalis wrightii(r = -0.693, p＜ 0.01), A. retroflexus(r = -0.724, p＜0.01), S. altissima(r = -0.728, p＜0.01), Eclipta prostrata(r = -0.779, p＜0.01) 순으로 감소하는 경향이 큰 것으로 조사되었고 평균발아일수도 처리구 농도가 증가함에 따라 발아하는데 소요되는 시간이 증가 되었으며(r=0.769, p＜0.01) 공여체식물과 검정식물에 따라 약간의 정도 차이를 보였다. 또한 공여체식물의 수용성 추출액 농도가 증가함에 따라 유식물의 지상부의 길이(r = -0.587, p＜0.01), 지하부의 길이(r = -0.741, p＜0.01), 생체량(r = -0.574, p＜0.01)과 뿌리털의 발생도 감소하였다. 한편 공여체식물의 수용성추출액 농도 증가에 따른 검정 병원균의 생장은 Botrytis cinerea(r = -0.266, p＜0.05), Diaporthe citri(r = -0.323 p＜0.01), Colletotrichum gloeosporioides(r = -0.512, p＜0.01), Pythium ultimum(r = -0.581, p＜0.01), Rhizoctonia solani(r = -0.806, p＜0.01) 순으로 생장이 억제되었다. 제초 및 항균활성을 보이는 수용체식물의 총 페놀 함량은 S. altissima 17.3±0.5mg/g, A. retroflexus 13.1±0.3mg/g, P. wrightii 12.0±0.4mg/g, S. spinosa 9.5±0.1mg/g, E. prostrata 4.1±0.1mg/g 순으로 분석되었다. 이들 결과를 종합하면 귀화식물인 수용체식물들은 자생식물과의 경쟁을 함에 있어 알레로패시 효과를 나타내는 페놀 화합물 등이 수관 내 토양으로 방출하여 하부식생에 대한 발아 및 생장과 토양미생물 생장 등에 영향을 주기 때문에 경쟁적 우위를 점하고 있으며, 천연제초제 · 살균제로서의 활용 가능성을 가지고 있는 것으로 판단된다.

본 연구는 고삼, 정향 및 neem 추출물에 대한 항균 · 항충력을 규명하여 벼 친환경 재배포장에서 병해충 방제제로의 이용가능성을 검토하고자 수행하였다. 식물추출물에 대한 잿빛곰팡이균에 대한 항균력 실험결과 정향추출물이 가장 높은 활성을 나타내었으며, 벼멸구에 대한 살충력은 고삼추출물이 가장 높은 효과를 보였는데, 고삼추출물의 300배 희석용액에서도 100%의 살충 방제가를 나타내었다. 정향과 고삼추출물에 각각 목초액(10%)과 유화전착제(10%)를 혼합하여 친환경 벼 재배포장에 살포하고 병해충 발생률을 조사한 결과, 정향추출물 처리구의 병해 방제가는 무처리구(100%) 대비 50.8%, 해충 발생은 70.6%를 나타내었고, 고삼추출물 처리구는 무처리(100%) 대비 병해 방제가는 43.1%, 해충 방제가는 79.1%로 나타났다. 결론적으로, 고삼 및 정향 추출물은 벼 친환경 재배 시 병해는 약 50%, 충해는 약 70%를 감소시킬 수 있어서 친환경농자재로의 활용 가능성을 확인하였다.

This study was conducted to evaluate the allelopathic effect of aqueous extract of Ganghwa domestic mugwort (Artemisia spp.) on vegetables and its related allelochemicals. When the receptor vegetables, such as Chinese cabbage, lettuce, and red radish, were treated with aqueous extract obtained from Sajabalssuk (A. sp*I), Ssajuarissuk (A. sp*II) or Ssajarissuk (A. sp*III), their germination rate, leaf number, plant height, and root length were restricted with increasing concentration of aqueous extract. Allelopathic effect was the highest in radish, than lettuce and Chinese cabbage in order. The growth of topplant were more inhibited then root growth observing in restriction of plant height, root length, and chlorophyll contents. The plant height, the root length of red radish were 53.3 and 61.2% and their fresh weights were 19.8 and 26.4% compared to those of controls, respectively. A. sp*III showed the highest allelopathic effect among the donor plants. In HPLC analysis, 7 phenol compounds were identified in A. sp*I and A. sp*II, and, in A. sp*III, and hydroxybenzoic acid and phenylacetic acid were further identified as allelochemicals. It is considered that their plant growths were variously inhibited by the amounts and types of allelochemicals in aqueous extracts. To increase the productivity of farm land after cultivation of mugwort, these results can be useful to select the following field crops.