목적 : 본 연구는 거주시설에서 생활하는 지적장애인들을 대상으로 도전적 행동의 발생빈도와 손상정도를 알아보고자 하였다. 연구방법 : 강원지역 사회복지 거주시설 13개 기관에서 생활하는 지적장애인 419명을 대상으로 도전적 행 동을 조사하였다. 도전적 행동 측정은 한국형 문제행동 평가척도(Korean version of Behavior Problem Inventory; KBPI-01)의 평가도구를 사용하여 자해행동, 상동행동, 공격/파괴행동으로 구분하 여 각 행동별로 발생 빈도(frequency)와 손상정도(severity)를 측정하였다. 결과 : 전체 대상자 중에서 도전적 행동의 행동발생 빈도는 자해행동이 53.2%, 상동행동이 68.3%, 공격/파괴 행동이 54.7%로 최소 월 1회 이상 나타났다. 또한 도전적 행동의 손상정도는 자해행동이 53.2%, 상동행동 이 67.8%, 공격/파괴행동이 54.7%로 보고되어 행동빈도와 손상정도가 높은 상관관계를 가지는 것으로 나 타났다. 지적장애의 손상수준이 가장 심각한 최고도(profound) 지적장애 그룹이 자해행동과 상동행동의 발생빈도와 손상정도가 가장 높게 나타났고 공격/파괴행동은 명시되지 않는(unspecified) 지적장애 그룹은 공격/파괴행동에서 가장 높은 발생빈도와 손상정도가 나타났다. 연령이 낮은 그룹(3~20세)에서 자해행동 이 59.4%, 상동행동이 73.5%로 가장 높은 발생빈도를 보였고 공격/파괴행동은 51~74세 그룹에서 64.0% 로 가장 높게 보고되었다. 결론 : 본 연구는 장애인거주시설에 생활하는 지적장애인들의 도전적 행동을 자해행동, 상동행동, 공격/파괴 행동으로 구분하여 각 행동별 발생빈도와 손상정도를 조사하였다. 이를 통해 지적장애인들이 보이는 도 전적 행동의 심각성에 대해 인식하고 이를 중재할 수 있는 효과적인 치료 지원 서비스 도입이 필요할 것이다.

The purpose of this study was to compare hand washing awareness and microorganism contamination on the hands for evaluating the difference between awareness and conditions of hand hygiene. The average number of total aerobic bacteria and coliform was 2.7 log CFU/hand and 1.8 log CFU/hand, respectively. Coliform bacteria were detected in all of the students tested in this study. The questionnaire survey data obtained via a direct interview were collected from 100 university students with microorganism analysis. A majority (90%) of the students recognized that good hand hygiene is important in order to prevent food poisoning. In the hand washing survey data, the responses suggesting “always washed” from “before eating food”, “after handling money”, “after finishing study” and “after visiting patients” were observed in 18, 13, 15 and 33 out of the 100 students, respectively. The questionnaire data of hand washing frequency and time duration showed that 14 students and 6 students answered “more than 8 times of hand washing per day” and “more than 30 seconds of hand washing time”, respectively. These results indicated that improper hand washing practices and poor hand hygiene were identified in the majority of the students. This emphasizes the difference between awareness and conditions of hand hygiene. Repeated hand washing education about the frequency, time duration and methods is constantly required to improve hand hygiene. Installation of hand washing equipment in the space of a restaurant and cafeteria is suggested for enhancing hand hygiene before eating food.

병원성 대장균군은 전세계적으로 식중독을 발생하는 중요한 병원균으로 알려져 있다. 국내 유통중인 생닭과 조리된 닭가공품 356시료에서 대장균 80균주(22.5%)를 분리 하여 병원성 유전자 multiplex PCR를 이용하여 분석한 결 과 astA 유전자가 26균주(32.5%)에서 검출되었으며, escV 유전자는 17균주(21.3%) eaeA 유전자는 16균주(20.0%) 검 출되었다. 특히 식품에서 문제가 되는 장출혈성 대장균 유 전자 stx 1는 3균주(3.8%)와 stx 2, EHEC-hly는 각 1균주 (1.3%)에서 검출되었다. 병원성 유전자를 토대로 STEC, EPEC, EHEC, EIEC or EAEC의 병원성 대장균으로 분류 한 결과 45균주(56.3%)가 검출되었으며 typical EPEC, EIEC and ETEC의 병원성 대장균은 검출되지 않았다. STEC 병원성 대장균은 O152, O1, O116, O26, O25, O119, O153 혈청형이 분리되었다. 특히 생닭에서 병원성 대장균에 대한 검출률이 높으므로 생닭을 가공 조리 시 교차 오염이 발생하기 않도록 조리 도구, 조리 환경에 대한 철저 한 관리가 필요하다고 생각된다.

In Korea, a serious amphibian disease caused by the fungus Batrachochytrium dendrobatidis (Bd) has been reported from historical samples collected in the 1900s. In this study, we continue to evaluate the current prevalence of chytridiomycosis in the Korean Peninsula and we include imported frogs from America to our analysis. Non-invasive skin swabs were taken from 275 apparently healthy frogs, and Bd was detected in five free living frogs by the nested PCR protocol consisting of two species: Bombina orientalis and Rana catesbeiana, from Gyeongnam and Cheonbuk provinces. These frogs comprised about 2% of the total number of free living samples. This study might be useful for understanding amphibian chytridiomycosis in Korea.

목 적: 본 연구는 제5기 국민건강영양조사(2012) 자료를 이용하여 한국 성인의 건성안 유병률과 위험요 인을 알아보고자 하였다. 방 법: 19세 이상 성인 중 안과의사에게 건성안 검진을 받은 5,698명(남자 2,368명, 여자 3,330명)을 대 상으로 인구사회학적 특성, 전신질환 특성, 건강관련행위 특성에 따른 건성안의 유병률과 위험요인을 분석 하였다. 결 과: 전체 연구대상자의 건성안 유병률은 13.5%이었다. 인구사회학적 특성별 건성안 유병률은 남성보 다 여성이, 50세 미만군보다 50세 이상군이, 농촌지역보다 도시지역에서 통계적으로 유의하게 높았으며, 전 신질환별 건성안 유병률은 고혈압, 이상지혈증, 골관절염, 류마티스 관절염, 우울증, 신부전증이 있는 경우 에 유의하게 높았다. 건강관련행위별 건성안 유병률은 비흡연자, 비음주자, 수면시간이 짧은 군이 높았다. 건성안 빈도는 여성에서(OR 2.29, CI 1.89-2.77)와 50세 이상군에서(OR 1.21, CI 1.01-1.46), 농촌지역에 서(OR 1.96, CI 1.56-2.47)에서 더 높았다. 유의한 모든 변수를 보정한 후에 건성안의 위험인자는 신부전 (OR 2.82, CI 1.48-5.69), 갑상선(OR 1.62, CI 1.16-2.26), 류마티스 관절염(OR 1.54, CI 1.01-2.33), 이상 지혈증(OR 1.42, CI 1.13-1.78), 우울증(OR 1.36, CI 1.11-1.66), 골관절염(OR 1.27, CI 1.02-1.57)이었다. 결 론: 우리나라 19세 이상 성인의 건성안 유병률은 13.5%으로 높은 수준이며, 50세 이상, 도시지역 거 주자, 여성과 신부전증, 갑상선, 류마티스 관절염, 이상지혈증, 우울증, 골관절염 환자에서 건성안의 위험이 높으므로 해당 위험요인을 가진 사람들을 대상으로 한 교육과 홍보를 통해 건성안증상을 조기 발견하여 적 절하게 대처하도록 할 필요가 있다

The purpose of this study was to investigate the microbiological contamination of water and drinking cups in springs and to estimate the toxin gene, enterotoxin production ability and antibiotic susceptibility of foodborne pathogens. Ten spring water and 34 drinking cups were tested. The average number of total aerobic bacteria and coliform bacteria in spring water were 1.8 log CFU/mL and 1.2 log CFU/mL, and in drinking cups were 4.7 log CFU/ 100 cm2 and 1.7 log CFU/100 cm2. Salmonella spp., Staphylococcus aureus, E. coli O157:H7, Listeria monocytogenes, Vibrio parahaemolyticus, Yersinia enterocolitica were not isolated from all of samples but Bacillus cereus was detected in 5 (14.7%) of 34 drinking cups. The nheA and entFM genes were major enterotoxin genes in B. cereus isolated from drinking cups. All of B. cereus tested in this study produce non-heamolytic enterotoxin but only 2 isolates possessed heamolysin BL enterotoxin producing ability. B. cereus was resistant to β-lactam antibiotics. These results revealed that the sanitary conditions of drinking cups in spring should be improved promptly. The substitution carrying a personal drinking cup for the public drinking cups equipped in springs is suggested to prevent food-borne illness.

Shiga toxin-producing Escherichia coli (STEC) is a major foodborne bacterial pathogen that causes many zoonotic diseases such as mild diarrhea, hemorrhagic colitis, and a life-threatening hemolytic uremic syndrome. STEC produces one or more Shiga toxins (Stxs) with or without other virulence factors including the locus of enterocyte and effacement pathogenicity island and the 60-MDa plasmid. Because cattle are the principal reservoirs of STEC, the consumption of undercooked meat and dairy products contaminated with bovine feces is the most common transmission route of STEC in human infections. To understand the epidemiology of STEC infection in South Korea, the prevalence of STEC among bovine feces, meat products, and human patients was reviewed in this study. We found that (i) the prevalence of STEC O157:H7 (hereafter referred to as O157 STEC) isolates was generally decreased, whereas non-O157 STEC isolates be increased among bovine feces and meat products from 2000 to 2012 and (ii) no severe human outbreaks occurred. Instead, 50 to 100 sporadic cases of STEC infection per year have been reported in asymptomatic human patients or patients with mild diarrhea. Key words: Shiga toxin-producing, Escherichia coli, STEC, prevalence, South Korea

Population density of Culex tritaeniorhynchus was annually monitored to predict the possibility of a Japanese encephalitis (JE) outbreak at 10 collection sites throughout Republic of Korea (ROK) during mosquito season from 2011 to 2015. Prevalence of Cx. tritaeniorhynchus in ROK was spatially and timely very variable and was significantly highest at Busan city during August. Monthly average population density of Cx. tritaeniorhychus showed high correlation to the monthly average daily average temperature and monthly average precipitation. Two models for the estimation of occurrence of Cx. tritaeniorhynchus based on annual monthly daily average temperature and monthly precipitation are shown with linear regression equations of exp(0.413×temperature-0.949) and exp(0.01258×precipitation+3.777). JE vector surveillance and vector control is warranted as part of an effective JE management program at ROK.

According to the regulations of the Korean Animal and Plant Quarantine Agency (QIA), offals include a greater diversity of internal organs than do those of other nations. The present study was conducted to investigate the microbiological quality of diverse edible offal products from pig and cattle slaughterhouses in Korea by performing aerobic plate counts (APCs) and E. coli counts. There were significant differences in E. coli values between red offal and white offal in both pigs and cattle. Significant differences in APCs were also observed between partial red offal and white offal of pigs (P < 0.05). However, no significant differences in APCs between red offal and white offal of cattle. The most frequently detected foodborne pathogen was Staphylococcus aureus (5.6% prevalence in pig offal and 12.5% % prevalence in cattle offal) followed by Clostridium perfringens (11.1 and 7.1%, respectively) and Salmonella (5.6% and 7.1%, respectively). None of the offal samples tested positive for E. coli O157:H7.

목 적: 성장기 아동 및 청소년기에 있어 난시유병률과 굴절이상도 분석을 통해 안보건 및 눈 관리의 필 요성을 인식시키고자 한다. 방 법: 보건복지부 산하 질병관리본부에서 시행한 2010 국민건강영양조사 자료를 통해 만 5세에서 만 18 세의 성장기 아동 및 청소년 총 280명을 대상으로 난시 유병률, 난시굴절력, 난시축, 구면굴절력을 분석하 고 난시교정굴절력이 교정시력에 미치는 상관성을 SPSS 18.0을 이용하여 유의 수준 p<0.05로 처리하였다. 결 과: 연령별 난시 유병률은 5-6세 50.0%, 7-9세 33.8%, 10-12세 48.8%, 13-15세 67.7%, 16-18세 63.4%를 나타내었고, 연령대가 증가할수록 난시 유병률은 증가하는 경향을 보였으며, C-1.00D 이하일 때 구면굴절력이 큰 경우 교정시력은 0.93, 난시굴절력이 큰 경우 교정시력은 0.83으로 유의한 결과를 나타내 었으며 교정시력에는 난시굴절이상도가 더 많은 영향을 주었다. 결 론: 난시는 교정시력에 영향을 주어 약시를 유발할 수 있기에 난시유병률 증가에 대한 중요성을 인식 하여 국민의 안보건과 시력저하 방지를 위한 제도적 장치와 사회적 관심이 더욱 필요할 것으로 판단된다.

어린이집 유아들이 사용하는 칫솔 및 양치 컵의 미생물오염도와 분리된 식중독 미생물의 독소 유전자 및 독소생산능력을 분석하여 칫솔과 양치 컵에 의해 발생할 수 있는 식중독을 예방하고자 칫솔 75개, 칫솔걸이 29개, 양치 컵 65개를 실험하였다. 일반세균수는 평균 5.3 log CFU 검출되었고 칫솔은 평균 6.7 log CFU 검출되어 가장 높은 오염도를 나타내었다. 대장균군은 칫솔 75건 중 41건 (54.7%), 칫솔걸이 29건 중 13건 (44.8%), 양치 컵 65건 중29건 (44.6%)에서 검출되었으며 진균수는 평균 3.2 log CFU 검출되었고 칫솔이 평균 4.6 log CFU 검출되어 칫솔의 대장균군, 진균 오염도가 가장 높게 나타났다. Salmonella spp.는 모든 시료에서 검출되지 않았으나 B. cereus는 칫솔 75건 중 1건 (1.3%), 양치 컵 65건 중 2건 (3.1%)에서 검출되었고 S. aureus는 양치 컵 65건 중 1건 (1.5%)에서 검출되었다. 칫솔에서 분리된 B. cereus의 경우 nheA, nheB, nheC, hblC, hblD, hblA, entFM 등 7종의 설사독소 유전자가 검출되었고 양치 컵에서 분리된 B. cereus 2균주는 nheA, nheB, nheC, entFM 설사독소 유전자만 검출되었다. 칫솔에서 분리된 B. cereus의 경우 HBL, NHE 설사독소를 생산하였고 양치 컵에서 분리된 B. cereus 2균주는 모두 NHE 설사독소만을 생산하였다. B. cereus는 β-lactam계 항생제에 내성을 나타내었고 S. aureus는 ampicillin과 penicillin에만 내성을 나타내었다. 이러한 결과는 대부분의 칫솔과 양치 컵이 양치 후 젖은 상태로 화장실에 보관되거나 젖은 상태로 자외선 살균고에서 살균되는 등 부적절한 보관가 살균방법이 주요 원인으로 분석되었다. 따라서 면역력이 취약한 어린이집 유아가 사용하는 칫솔 및 양치컵을 건조한 후 자외선 살균고 등을 이용 살균하여 상대습도가 낮은 건조한 곳에 보관하여야 할 것으로 판단되었다.

This study was conducted to elucidate the occurrence area and the density of mosquito larvae in Eunpyeong Newtown, Seoul, Korea. Sampling of mosquito larvae was carried out 2 times using a dipping method at 130 points in august, 2014. A total of 1977.6 individuals were collected. Aedini larvae such as Stegomyia albopicta (Skuse) (=Aedes albopictus) or Hulecoeteomyia koreica (Edwards) (=Oclerotatus koreicus) were the most frequently collected than Culex spp. and the Anopheles spp. Mosquito larvae were surveyed in the street inlet (a rain drip box). The positive correlation of the larval density was found with the street inlet point with leaf mold and the depth of water. In the result of spatial statistics analysis, all points larvae collected were clustered and the tendency of spatial autocorrelation was apparent, but points over 20 individuals were dispersed. This result can be assumed that the mosquito larvae are able to occur to stagnant water in all places of this area. To control to mosquito occurrence, we think integrated methods are needed such as biological, ecological, chemical, physical control methods in various aspects.

Purpose: To asses the prevalence of refractive error in school-aged children. Methods: A total of 735 school-aged children in Jenman province were included. In all participants, visual acuity, auto Ref-Keratometer, and subjective refraction were determined. A spherical equivalent of -0.5 diopter(D) or worse was defined as myopia, +0.5D or more was defined as hyperopia, and a cylinder refraction greater than 0.75D was defined as astigmatism. Myopia was categorized as either low myopia -0.75∼ -2.00D, medium myopia -2.00 to -6.00 D or high myopia -6.00D or greater. Hyperopia was categorized as low hyperopia +0.75D to 2.00D, and medium hyperopia +2.00D or more. Results: The prevalence of presenting, spectacle corrected visual acuity of 0.5 worse eye was 28%, 48.2%. The prevalence rate of myopia, hyperopia, astigmatism were 43.7, 7.6%, 12.1%. The prevalence of myopia was the highest and followed by the prevalence of astigmatism. The prevalence of hyperopia decreased with age. Conclusion: Compared with other reported rates, the prevalence of myopia in the school-aged children of Jennam is similar to that in most places excluding the other countries, and that hyperopia is in the mid range.

Salmonellosis is a significant public health concern and a major cause of bacterial foodborne poisoning in Korea. Approximately 95% of salmonellosis cases in humans are related to the intake of contaminated food products, which mostly originate from livestock such as poultry, pigs, and cattle. To successfully develop strategies to reduce the spread of Salmonella, it is important to investigate the prevalence of Salmonella spp. isolated from food animals and meat. In Korea, the prevalence of Salmonella spp. in cattle was 1.2 2.0%, whereas in pigs it was 1.5 21%. Of all livestock and meat products analyzed, the Salmonella isolation rate was the highest in chickens (18.8 30.3%) and their meat. Antibiotic resistance is an important social issue. The frequent use of antibiotics in the livestock industry has led to increased antibiotic resistance in Salmonella isolates that are extracted from livestock, which raises serious public health concerns. To prevent the spread of antibiotic resistance, it is essential to collect data related to antibiotic-resistant Salmonella strains isolated from food animals and meat. Antibiotic resistance tests revealed that Salmonella isolated from cattle, pigs, and poultry were highly resistant to tetracycline and streptomycin. Recent studies show that the overuse of antibiotics in food animals can also affect antibiotic resistance in humans. Therefore, it is important to establish an appropriate management strategy to successfully prevent misuse of antibiotics within the Korean livestock industry.

Canine atopic dermatitis (CAD) is an allergic skin disease with characteristic clinical features associated with immunoglobulin E (IgE) antibodies. Identification of the causative allergens is the diagnostic goal, which is essential to treat and manage CAD patients. CAD is commonly associated with environmental allergens surrounding the patients. For this reason, it is important for diagnostic tests to select allergens that are related to the environment of each country and each province. There are two main allergen-specific tests, serological IgE test (SAT) and intradermal skin test (IDT). SAT did not show direct cutaneous reaction but did show serological reaction against allergens. However, SAT is simpler and more convenient than IDT in small animal practice. In this study, we selected domestically prevalent allergens for SAT, including 60 food allergens and 60 inhalant allergens, and tested eight dogs tentatively diagnosed with CAD based on Favrot’s criteria. Furthermore, IDT was performed on four dogs from the SAT group for comparison of SAT and IDT, and the results were very similar. In SAT, four types of mites (Bloomia tropicalis, Glycophagus domesticus, Euroglyphus maynei, and mite mixture 1 Korea; house dust mites), four types of molds (Botrytis cinerea, Alternaria alternata, mold fungi mixture 11, mold fungi mixture), and one type of pollen (tree pollen mix 3 Korea) induced a reaction in more than half of dogs tested. In IDT, all four dogs reacted positively to Dermatophagoides farinae, and three reacted positively to Dermatophagoides pteronyssinus and house dust. The mean agreement rate between SAT and IDT in this study was 76.3%. This is the first trial to apply local allergens for SAT in Korean veterinary medicine, and it might play an important role for diagnoses and management of animal allergic diseases.

목 적: 황반변성의 유병률 변화예측과 사회적 지표에 따른 비교를 통해서 황반변성을 일으킬 수 있는 요 인을 알리고자 한다. 방 법: 2012년에 보건복지부 산하 질병관리본부가 시행하여 발표된 국민건강영양조사 자료를 통해 2008 년에서 2012년까지의 자료를 분석하였다. 대상자는 18007명이며 SPSS(Ver12.0)를 사용하였다. 결 과: 지난 5년간 연령별 황반변성의 유병률은 40~49세 1.3%, 50~59세 4.5%, 60~69세 10.1%, 그리 고 70세 이상에서는 16.3%로 나이가 증가함에 따라 유병률이 증가하였다(p=0.00). 남성은 50~59세와 60~69세 연령층에서 5.0%의 가장 큰 유병률 차이를, 여성은 60~69세와 70세 이상의 연령층에서 7.7%의 가장 큰 유병률 차이를 나타내었다. 2012년 지역별 황반변성 유병률은 동 지역 4.2%(n=171)와 읍 지역 6.3%(n=63)(p=0.00), 교육수준별 황반변성 유병률은 초등학교 9.8%(n=113), 중학교 6.0%(n=31), 고등학교 3.0%(n=51), 그리고 대학교 1.8%(n=18)를 각각 나타내었다. 결 론: 60~69세 이후부터는 성별간의 황반변성 유병률 차이가 큰 것으로 나타났다. 이와 함께 안질환과 보건교육에 대한 기초지식이 낮은 저학력층과 농어촌에 거주하는 세대의 황반변성에 대한 적극적인 관리가 필요할 것이다.

본 연구에서는 식품에서의 L. monocytogenes에 대한 기 초자료를 제공하고자 2010년부터 2011년까지 서울지역에서 수거된 식품 총 1042건에서 L. monocytogenes를 분리 하여 항생제 감수성 검사를 하였다. 김밥 4건(0.8%), 육회 4건(2.6%), 연어제품 2건(11.1%), 돈육식품과 조리된 생선에서 각 1건씩(각5.9%, 6.3%) 총 12균주가 분리 동정되었다. 분리 균주를 20종의 항생제에 검사한 결과 대다수 항생제(16종)에 감수성을 나타내었지만 cefotetan (11균주), cefotaxime (7균주), cefepime (6균주)에서 내성을 나타냈으며 3균주에서 임상치료에 자주 사용되는 tetracycline에 대해서 내성을 나타내었다.

The objective of this study was to identify the nutritional risk factors by blood analysis, in 1,083 preschool children of age 3 to 6 years. The frequency of anemic children was 7.3% with Hb<11.1 g/dL, 29.9% with ferritin<20 ng/mL, and 16.7% with transferrin Fe saturation(%)<15%. The prevalence of anemia in these children were 12.8% for those with MCV<79 fL, and it was 71% for those with TIBC>400 μg/dL. Serum ferritin concentration was 20 ng/mL in the normal children. Thirty two percent of the children had anemia with Hb<12 g/dL, which is below the normal range of Hb. Exactly 15.4% of the children had serum Fe concentration of 60 μg/dL. The transferrin Fe saturation of the children (16.3%) was >15%. The serum ferritin concentration showed low correlations with Hb, Fe, transferrin Fe saturation, and MCV. The transferrin Fe saturation, higher Hb concentration, MCV, and Hct values were increased significantly. Consequently, iron-deficiency anaemia was thus defined as having Hb concentration <12 g/dL accompanied by ferritin concentration <20 ng/mL or Hct <33%.

The purpose of this study was to compare the validity of obesity indices among the body mass index (BMI), waist circumference (WC), and body fat percentage (BF%), and to determine which is the most useful index to predict the risk of chronic diseases of elderly people. This study was conducted as a cross-sectional study at welfare centers in Seoul. The total number of subjects was 261 (68 men and 193 women) with age ≥60 years. The distribution of obesity using 3 obesity indices in the subjects with hypertension, diabetes, or arthritis was BF%>WC>BMI in elderly men and WC>BF%>BMI in elderly women. In elderly women, odds ratios (ORs) for hypertension in BMI and WC quartiles were significantly increased in quartile 2 and 3 (p<0.05). The ORs for hypertension, hyperlipidemia, and arthritis in BF% quartiles were significantly increased in quartile 3 and 4 (p<0.05). The BF% was sensitive obesity index for predicting the occurrence of chronic disease in men, and the WC was sensitive index in women. Our results suggested maintaining BMI less than 23.5 kg/m2, WC less than 82 cm, and BF less than 35% in order to prevent chronic diseases in elderly women.