목 적: 백내장 유병률 변화와 예측을 통해 백내장의 위험성과 관리 및 예방의 중요성을 알리고자 한다. 방 법: 2008년에서부터 2012년까지 최근 5년간 보건복지부 산하 질병관리본부가 시행하여 발표한 국민건강영양조사 자료를 통계 분석하였다. 결 과: 연령별 백내장의 유병률은 40-49세 11.1%, 50-59세 35.7%, 60-69세 71.8%, 그리고 70세 이상에서는 94.2%로 나이가 증가함에 따라 유병률이 증가하였으며(p=0.00), 특히 남성 50-59세 연령층의 경우 2008년 35.7%에서 2012년 48.2%로 5년 동안 13.0%의 백내장 유병률이 증가한 것으로 나타났다. 지역별 백내장 유병률은 동 지역에서는 39.5%와 읍 지역에서는 51.1%로(p=0.00), 소득별 백내장의 유병률은 최하위 소득군 43.1%, 중하위 42.9%, 중상위 41.6% 그리고 최상위 40.7%를 각각 나타내었다. 결 론: 65세를 넘어가면 남성과 여성간의 백내장 유병률의 유의한 차이가 있는 것으로 나타났다(p=0.02). 그러나 그 차이는 계속 줄어들고 있으며 그것은 특히 50대 남성 백내장 유병률의 가파른 증가와 연관이 있음을 알 수 있다.

Purpose: To examine the relationship between working in a very dry clean room (relative humidity less than 1%) and dry eye, and its progress by lapse of time. Methods: Targeting 352 employees working in a clean room of onesecondary cell production plant, it performed a dry eye examination in 2011, 2012, and 2013, totally three times. It performed the TFBUT, Schirmer’s test,slit lamp microscope examination, and Mac Morning survey. dry eye stages were classified based on the DEWS criteria. The annual examination results were analyzed through the Anova test and the post-mortem was conducted with Tukey, and in order to learn the effect of working time on dry eye, anunivariate logistic regression analysis was conducted having dry eye examination results as a dependent variable and working time as an independent variable. Results: According to TFBUT and Mac Morning survey, dry eye degenerated as time passed. There was no change in the Schirmer’s test. Considering that as a result of the univariate logistic regression analysis having dry eye examination results as a dependent variable and working time as an independent variable, in the Mac Morning survey, the cross ratio increased up to 1.127(95%CI 1.033~1.230)by lapse of time, it could be found that as working time is elongated, the complaining rate of dry eye increases. Conclusion: It is thought that a clean room with a relative humidity less than 1% affects causing dry eye. Working time limitation, job rotation, and health care such as prescription of artificial tear solution without preserved agent are necessary.

Listeria monocytogenes continues to be a important food safety concern. The aims of the present study were to investigate the prevalence and antimicrobial susceptibility of L. monocytogenes. A total of 1,042 samples was collected from 2010 to 2011 in Seoul and twelve L. monocytogenes were isolated. Isolation rate was Gimbap (0.8%), Beef (yukhoe) (2.6%), Pork (cooked) (5.9%), Fish(cooked) (6.3%), and Salmon (11.1%), respectively. In this study, most of the isolates were susceptible to antibiotics. The most common resistance was cefotetan on 11 isolates, followed by cefotaxime on 7 isolates, cepefime on 6 isolates and tetracyclin on 3 isolates.

Most Korean beekeepers have moved from south to north of Korea to collect nectar from black locust (Robinia pseudoacacia) flowers for 2 months. This provided a valuable opportunity to sample bees originating from diverse areas in one location. We initiated a survey of honeybee (Apis mellifera) colonies on the blooming period of Acacia to determine the prevalence of Nosema apis and black queen cell virus (BQCV) in 2013. Nosema causes significant losses in population size of honeybees. Sixteenth hives were sampled for this study. Bees were collected on the 4th and 13th of May, 2013. Nosema spore counts ranged from zero to 1,948,333 spores per bee. The average number of nosema spores per bee was calculated to be 450,000. Approximately 94% of the apiaries examined were infected with nosema, based on the presence of spores in the flowering period of Acacia. Also nosema is thought to be associated with black queen cell virus. RT-PCR analysis shows that BQCV infection rate was 100%. This indicates that nosema and BQCV is the predominant species affecting honeybee colonies.

Visceral larva migrans (VLM) is a clinical syndrome caused by the migration of certain larval nematodes through the tissues of an aberrant host. VLM in humans is most frequently associated with Toxocara canis, the common roundworm of dogs. To demonstrate the prevalence of Toxocara spp. eggs in public playgrounds in Seoul city, soil samples collected from April to December in 2012 were examined. Eggs of parasite were found 4 of 1,174 in soil samples from public playgrounds and educational facilities in Seoul. Of the 1,174 soil samples examined, 0.34% of samples were found th be positive ova of Toxocara canis. Soil samples in educational facilities were more contaminated (0.57%) with the eggs than those in public playground area (0%). Most Toxocara canis eggs in sands were found in the autumn.

TM7 is an uncultivated organism which is present in ex- tremely diverse environments. Members of the Dialister genus are difficult to culture as a result of which many of these strains remain uncultivated. It has been suggested that TM7 and Dialister bacteria may belong to a group of suspected periodontal pathogens. In our current study, the presence of the sebacteria in Korean dental plaque samples was asses- sed using PCR detection methods with specific primers for 16S ribosomal RNA genes. The experimental group inclu- ded 84 volunteers (35 males and 49 females). Plaque sam- ples were collected from 4 non-adjacent proximal sites of the molar areas of the mandible in each subject and pooled. TM7 was detectable in 56% and the Dialister genus in 27.5% of the volunteers. Both TM7 and Dialister were present in 20.3% of volunteers. We found that 36.9% of the volunte e rs we re negative for both bacteria. Further s tudi e s to evaluate the prevalence of these putative pathogenic bac- te ria in the Korean population are warranted.

어린이집 유아들이 공용으로 사용하는 수건의 미생물 오염도와 분리된 식중독 미생물의 장독소 유전자 및 장독소 생산 특성을 분석하여 어린이집 유아 사용 수건의 위생 안전성을 확보하고자 어린이가 손 씻은 후 공용으로 사용 하는 수건 22개 (사용 전 7개, 사용 중 15개)를 연구대상으로 하였다. 일반세균수는 평균 6.2 log CFU/100 cm2로 검출되었고 진균수는 평균 4.1 log CFU/100 cm2로 검출되었 으며 대장균군은 사용 전 수건의 경우 7건 중 4건 (57.1%), 사용 중인 수건의 경우 15건 모두에서 (100%) 검출되었 다. 어린이 사용 수건의 미생물 오염도가 높게 나타나 수 건의 위생적인 살균 세탁 및 상대습도가 낮은 곳에 보관하는 등의 보관 방법 개선이 필요한 것으로 판단되었다. Salmonella spp.의 경우 실험에 사용된 모든 수건에서 검출되지 않았으나, Staph. aureus는 22건 중 5건 (22.7%), B. cereus는 11건 (50.0%)에서 검출되어 B. cereus 오염이 가장 심각한 것으로 나타났다. Staph. aureus 장독소 유전 자와 독소 단백질은 모두 불검출 되었으나 분리 동정된 11균주의 B. cereus 장독소 유전자 실험 결과 hblC, hblD, hblA 유전자가 각각 72.7, 72.7, 54.5% 검출되고 nheA, nheB, nheC 유전자가 각각 81.8, 72.7, 54.5% 검출되었으며 cytK, entFM 장독소 유전자는 각각 45.5, 90.0% 검출 되었다. B. cereus 장독소 실험결과 HBL 장독소는 11균주 중 8균주 (72.7%), NHE 장독소는 5균주 (45.5%) 검출되었고 HBL과 NHE 장독소 중 하나 이상을 생산하는 B. cereus 균주는 10균주 (90.9%)로 나타났다. 수건에 오염된 B. cereus 균주의 교차오염에 따른 식중독 위험성이 상존 하는 것으로 나타나 개인별 수건 또는 종이 타올 사용을 고려하여야 할 것으로 판단되었다.

목적: 본 연구는 우리나라 성인의 건강형평성 주요 척도에 따른 백내장을 분석함으로써 눈 건강 형평성을 평가하고자 하였다. 방법: 국민건강영양조사(2009) 안검진 조사 데이터를 이용하여 무수정체안을 제외한 만 20세 이상의성인7,412명을 대상으로 소득수준, 교육수준, 직업, 지역에 따른 백내장의 유병률 및 오즈비를 분석하였다. 백내장은 안과전문의가 slit-lamp를 이용하여 검사하였다. 건강형평성 주요 척도에 따른 유병률은 t-검정 및 x² 검정하였고, 오즈비(odds ratio)는 로지스틱 회귀분석을 이용하였으며, 유의수준은 0.05로 PASW18.0을 사용하여 분석하였다. 결과: 백내장의 표준화 유병률은 26.4%이었다, 유병률은 가구소득‘하’에서41.2%, ‘초졸 이하’59.9%.‘무직’53.9%,‘도시지역’62.8%이었다. 건강형평성 주요 척도에 따른 백내장의 오즈비는 50세 이상군에서 가구소득이 ‘하’일 때 2.60(CI, 196-344), ‘무직군’일 때 2.33(CI, 1.49-3.34), 농촌지역에서 1.24(CI, 0.99-1.56)로 높게 나타났다. 20-49세군은 ‘초졸 이하’에서 5.48(CI, 2.71-11.06)이었고, ‘농림어업 숙련 종사지군’에서 .33(CI, 1.37-8.10)으로 높았다. 농촌지역에서 가구소득이 ‘하’일 때 오즈비는 2.33(CI, 1.35-3.69), ‘초졸 이하’일 때 2.03(CI, 0.97-4.26), ‘단순노무 종사지군’에서 1.28(CI, 0.52-3.14)로 도시지역에 비해 높았다. 여자의 경우 가구소득이‘하’에서 1.60(CI, 1.12-2.30), ‘초졸 이하’에서 1.79(CI, 1.09-2.92)로 남자에 비해 높게 나타났다. 결론: 낮은 사회경제적 상태에서 백내장의 유병률과 오즈비가 증가하여 우리나라 성인의 눈 건강 불평등이 존재하였으며, 이를 위해 저소득층을 위한 종합적인 일차안보건의료정책이 필요하다. 또한 외국의 안보건정책과의 비교를 통해 기존의 치료 위주의 보건정책보다는 예방과 교육 중심의 보건정책의 개발이 요구된다.

본 연구는 김포시에서 모기성충의 계절적 발생소장과 얼룩날개모기류 유충의 발생을 파악하기 위해서 실시되었다. 성충모기는 6지점에서 유문등을 이용하여, 2008년 5월부터 9월까지 조사되었다. 조사결과 8속 16종 48,919마리가 채집되었다. 금빛숲모기(Aedes vexans nipponii)가 43%로 가장 많았고, 중국얼룩날개모기(Anophels sinensis)가 34%, 작은빨간집모기(Culex tritaeniorhynchus)가 14%로 이 3 우점종이 전체의 91%를 차지했다. 얼룩날개모기류의 계절적 발생소장은 작은빨간집모기와 비교하여 다른 피크를 보였다. 얼룩날개모기류 유충은 203지점 중 138지점(63%)에서 확인되었다. 지점당 정량조사 결과에서 평균유충밀도는 연꽃 재배지가 6.9개체로 가장 높았으며, 다음으로 수로가 4.5개체, 미나리꽝이 3.4개체, 유수지가 3.2개체, 휴경논이 3.1개체, 논이 1.8개체, 습지가 1.4개체, 하천이 0.2개체 순이었다. 유충밀도와 용존산소량, 산도, 염분도, 흡밀원(축사, 돈사, 계사) 사이에 유의한 관계성은 없었다. 최근린평균거리분석(ANNA)의 결과에서, 유충 조사지점간 분포패턴은 전체 조사지점간과 4.0개체 이상 확인된 지점간에서 각각 밀집분포와 불규칙분포였다. 하성면에서는 유충이 3.0 개체 이상 확인된 지점간 분산분포를 나타냈다.

Dentists are known to be highly exposed to the musculoskeletal disorders (MSD). The present study investigated the prevalence of MSD among Korean dentists and association between their MSD and physical workload. In addition, detailed causes related to the physical workloads were identified in dental operation, and needs for improvement of dental instrument and environment were collected from Korean dentists. The standardized Nordic questionnaire was used to survey body troubles and three types of questionnaires were made to investigate the physical workloads, causes of the physical workloads and improvement priority of dental equipments, respectively. A total of 104 Korean dentists were participated in the survey. Neck trouble (82%) was the most prevalent in Korean dentists, and shoulder (68%) and low back trouble (56%) followed, while low back trouble was reported to be most frequent in previous studies. The body troubles were related to the physical workload of the corresponding body parts, although they were not associated with personal characteristics. Most Korean dentists selected ‘to keep direct view inside patient’s mouth’ and ‘no support of the hand with dental instruments’ as the causes of awkward and strenuous work postures. They wanted design improvement for some equipment in their operating room such as operating light and arrangement of workplace.

목 적: 본 연구는 한국인의 백내장과 녹내장의 유병률에 대해 알아 보고자 하였다. 방 법: 백내장과 녹내장의 유병률은 2007년도 국민건강영양조사 자료에서 30세 이상의 2,643명(남성: 1,101명, 여성: 1,543명)을 대상으로 하였다. 본 연구는 서베이분석법인 PROC SURVEYFREQ를 사용하여 사회경제적 위험요인과의 관계에 따른 유병률의 변화를 보았다. 모든 통계분석은 SAS 9.2 통계패키지를 사용하였으며, 유의성 검정은 0.05 이하로 하였다. 결 과: 2007년도 한국인은 3.0%(남성 9.8%, 여성 4.6%)가 백내장을 가지고 있었고, 7.8%(남성 5.7%, 여성 9.8%)가 백내장을 앓은 경험이 있었다. 녹내장 유병률과 경험률은 각각 0.2% (남성 0.4%, 여성 0.2%)와 0.5% (남성: 0.5%, 여성 0.4%)이었다. 65세 이상 남성 노인에서, 백내장 경험률 및 유병률은 19.9%와 4.1%이었으며, 여성에서는 38.3%와 17.2% 이었다. 그러나 30-64세 사이의 젊은 사람들에게 백내장 경험과 유병률이 남성에서 3.4%와 0.8%이었으며, 여성에서 1.6%와 3.0%이었다. 65세 이상 노인에서, 녹내장 경험률과 유병률은 남성에서 1.6%와 1.1%이었으며, 여성에서는 1.6%와 0.8%이었다. 젊은 사람들에게서 녹내장의 경험률과 유병률은 남성에서 0.3%와 0.1%이었으며, 여성에서는 모두 0.1% 이었다. 또한, 교육수준이나 소득이 낮은 경우, 비경제인구이거나 실업자의 경우 백내장과 녹내장의 유병률과 경험률이 높았다. 결 론: 30세 이상의 한국인에서 백내장의 유병률은 3.0%, 녹내장의 유병률은 0.2%였다. 본 연구결과는 한국인에서 안보건과 안질환의 적절한 건강관리 정책을 만드는데 있어 중요한 결과라고 할 수 있다.

Antibiotic resistance in animal isolates of enterococci is a public health concern, because of the risk of transmission of antibiotic-resistant strains or resistance genes to humans through the food chain. This study investigated phenotypic and genotypic resistances profile of tetracycline in 245 Enterococcus isolates from bovine milk. A total of 245 enterococci were isolated from 950 milk samples. The predominant strain was E. faecalis (n = 199, 81.2%) and E. faecium (n = 25, 10.2%). E. avium (n = 7, 2.9%), E. durans (n = 6, 2.5%), E. gallinarum (n = 4, 1.6%), and E. raffinosus (n = 4, 1.6%) were also isolated. Of the 245 enterococcal isolates 76.3% (n = 187) displayed tetracycline resistance (≥ 16 μg/ml). Of the 187 tetracycline-resistant isolates, 83.4% (n = 156), 16.1% (n = 30), and 26.7% (n = 50) possessed the genes tet(M), tet(L), tet(S) respectively. While 3.2% (n = 6) of the tetracycline- resistant isolates possessed all three genes tet(M) + tet(L) + tet(S), 8.6% (n = 16), 16.0% (n = 30), and 2.7% (n = 5) of them possessed two genes tet(M) + tet(L), tet(M) + tet(S), and tet(L) + tet(S) respectively. The tetracycline resistance pattern investigated in this study was attributable mainly to the presence of tet(M).

This study was conducted to evaluate the microbiological hazard on nursery school children's hands and to investigate the reduction effect of hand washing education. A total of 59 nursery school children's hands were tested. The average number of total aerobic bacteria was 3.72 ± 0.38 log CFU/hand. Five children's hands(2 male and 3 female) were positive(14.3%) for the coliform bacteria. These results showed that hand washing education are required repetitively. Among the pathogenic bacteria tested in this study, Staphylococcus aureus and Bacillus cereus were detected in 9(25.7%) and 16(45.7%) out of 35 their hands, respectively. Twelve out of sixteen B. cereus isolates(70.0%) produced enterotoxin. The results indicate that the hand hygiene of nursery school children needs to be improved. Comparing before and after hand washing in educated and non-educated group, the reduction effect of total aerobic bacteria on their hands was 0.42 and 0.60 log CFU/hand, respectively. The educated group showed 0.18 log CFU/hand higher reduction effect than non-educated group but microorganism did not eliminate perfectly. From the results, using a hand sanitizer after washing with soap and the continuous hand washing education are required to control the contaminated bacteria on nursery school children's hands.

In this study, the contamination levels of total aerobic bacteria, E. coli, total coliform and S. aureus of seasoned dried fishes (SDF) in Korea were investigated. A total of 81 SDF samples were purchased randomly from 28 stores. Contamination range of total aerobic bacteria, total coliform and S. aureus were 150~1,700,000, 10~31,000and 10~220 CFU/g, respectively. E. coli was detected in only one samples in the qualitative test. We have analyzed quantitatively Staphylococcal enterotoxins (SE-type A, C and D) produced by S. aureus contaminated in SDF using a TECRA kit and standard curve. The curve equation was Y = 0.1499 * X + 0.1183 and maximum amount of SEs in SDF was 0.71 ng/ml. Reduction speed of S. aureus in SDF stored at 37 ℃ was the highest among the samples stored for 8 days at different temperature of 7, 18 and 37℃ . On the basis of the results, SDF in Korea can be contaminated by a variety of pathogenic bacteria. Therefore, precautionary measures are necessary for consumer protection, including the improvement of sanitary conditions in the processing plants in Korea.

We investigated the prevalence of the Listeria monocytogenes from livestock processed products in processing plants and retail markets of Korea from 2010 to 2011. A total of 1,380 samples were collected; Meat processed products such as cooked ham and sausage, jerked meat, and meat extract products. Milk processed products such as milk, butter, cheese, and ice cream. Egg processed products such as whole egg liquid and pidan. L. monocytogenes were isolated from samples using listeria enrichment broth, fraser broth and Oxford agar, and counted in Oxford agar. The three of L. monocytogenes strains (1.16%) were isolated from sausages, two (0.73%) from mixed pressed ham and one (0.51%) from jerked meat, respectively. The colony forming unit (CFU) of L. monocytogenes from all samples were below 10 CFU/g. The four isolates (66.6%) were 1/2b except two isolates (1/2a) in the serotypes. Further studies are needed to understand the transmission route of L. monocytogenes, including a survey of food handlers, environments of retail markets, and all potential risk factors in cooked sausages, mixed pressed hams, and jerked meat processing.

목적: 우리나라 성인에서 흡연과 사회경제적 상태에 따른 백내장의 대응위험도를 평가하여 백내장의 유병인자를 알아보고자 하였다. 방법: 국민건강영양조사(2009년) 안검진 데이터를 이용하여 만 40세 이상의 성인 4,976명을 대상으로 흡연과 사회경제적 상태에 따른 백내장의 유병률과 대응위험도(odds ratios)를 평가하였다. 백내장 유병률의 차이는 t-검정 및 χ2 검정을 실시하였고, 백내장 유병과 사회경제적 요인간의 관계는 로지스틱 회귀분석을 이용하여 성별, 연령 및 체질량지수(BMI), 고혈압, 당뇨, 고지혈증을 보정한 대응위험도(OR)와 95% 신뢰구간(CIs)의 산출하였다. 결과: 백내장 유병률은 40대는 10.8%이었고, 60대는 73.8%로 급격히 증가하였으며, 80세 이상에서는 97.5%로 연령의 증가와 함께 높아졌다. 백내장의 대응위험도는 성별, 연령, 체질량지수, 고혈압, 당뇨, 고지 혈증을 보정하였을 때 교육수준이 초졸 이하인 군에서는 1.28(p=0.008 CI, 1.07-1.53), 100만원 이하의 저소득군에서는 1.31(p=0.004 CI, 1.09-1.57), 흡연군에서는 1.38(p=0.013 CI, 1.07-1.78)로 통계적으로 유의하였다. 결론: 우리나라 성인에서 교육수준과 소득수준이 낮고 흡연을 하는 집단에서 백내장 유병률과 대응위험도가 높았다.

Although several enteric viral pathogens including the porcine groups A, B and C rotaviruses (PGARV, PGBRV, and PGCRV), sapovirus (PSaV), and torovirus (PToV) are known to cause endemic diarrheas in weaning and post-weaning piglets, their precise prevalence in Korea is not clear. Therefore, we examined 1,222 diarrhea stools obtained from 627 farms during 2004～2009 by RT-PCR and/or nested PCR for evaluating their precise prevalence in Korea. PGARV was the predominant pathogen during 2004～2007 but its prevalence was markedly reduced during 2008～2009. PGBRV infections caused endemic diarrhea during 2004～2007, but was hardly detected during 2008-2009. PGCRV was detected at 27.0%, 14.5%, 42.4%, 28.8%, 7.3%, and 54.2% during each year of 2004～2009, respectively, indicating its high prevalence in Korea throughout the years. PSaV induced with high prevalence (32.4-39.2%) during 2004～2005 but its detection rate was markedly decreased during 2006～2009. PToV caused sporadic infections only during 2006 (1.0%) and 2007 (6.9%). These enteric viruses were detected in diarrhea specimens in piglets usually in combination with each other and/or together with bacterial pathogens including the Escherichia coli, Salmonella spp., Brachispira hyodysenteriae, and Lawsonia intracellularis. Infections with PGARV, PGCRV, PSaV, and PToV were more prevalent in fecal samples collected in cold seasons. These results provide important epidemiological data for the control and establishment of a surveillance system for the prevailing enteric viruses in Korea.