본 시험은 동계작물인 쌀보리의 알곡에 대한 사료로서의 활용도를 제고하고자 미생물 분포를 분석하고, 발효과정 중 이화학적 및 미생물학적 특성의 변화 분석을 통하여 효 과적인 발효사료 제조방법을 제시하고자 수행하였다. 즉, 수확된 쌀보리 종실에 시판 생균제를 기준에 맞게 접종한 후 공기와 접촉하지 않도록 밀봉하여 37℃에서 7일간 발 효를 통해 쌀보리 종실의 저장성과 안전성을 증진시킬 수 있다. 쌀보리 종실 자체에 Staphylococcus속과 Bacillus속의 미 생물들이 다수 존재하여 자연발효의 가능성이 있음을 보여 주었다. 또한 생균제를 접종하지 않은 쌀보리 종실도 기간 이 경과됨에 따라 산도가 저하되고 유산균 수가 증가하여 발효가 이루어지고 있는 것으로 나타났다. 그러나 쌀보리 종실에 생균제를 접종하였을 경우, 발효 7일차에 pH가 4.33±0.02로 발효가 더 빨리 진행되었으며, 유산균의 수도 전체 발효기간 동안 미 접종 대조구에 비해 높게 유지되었 으나, 효모의 수에 있어서는 차이가 나타나지 않았다. 발효 에 의해 쌀보리 종실의 산도가 저하됨에 따라 초기 107 cfu/g 이상 검출되었던 대장균이 발효 7일차 이후부터는 전 혀 검출되지 않아 발효가 쌀보리 종실의 안전성을 유지시 키는 데 있어 효과적인 저장방법으로 나타났다.
본 연구는 흑쌀보리 육성을 위해 Otello, 흰쌀보리 및 늘쌀보리 등 3품종으로 교배하고 계대 육성하여 F7세대의 각 형질을 분석한 결과는 다음과 같다.
F7세대에 대한 상관의 정도는 단백질 함량과 β-glucan 함량 간에서, β-glucan 함량과 까락 길이 간에서, β-glucan 함량과 1000립중 간에서, 이삭 길이와 1000립중 간에서 각각 유의하였다. 광의 유전력은 모든 형질에서 0.9292 이상으로 높았다. 경로계수 분석에서 1000립중에 관여하는 직접효과는 단백질 함량, β-glucan 함량, 이삭 길이가 정의 효과를 보였으며, 간접효과도 단백질 함량, β-glucan 함량, 이삭 길이의 관여 조합이 정의 효과를 보였다. 선발지수는 단일형질에서 까락 길이와 단백질 함량이 정으로 높았다. 3개 형질 및 4개 형질의 조합에서는 단백질 함량과 까락 길이의 관여가 모두 정으로 높았다.
본 연구는 우수한 흑쌀보리 육성을 위하여 동아대학교 종합농장 수도포장에서 실시하였다. 시험에 사용된 교배친은 무조성인 흑피맥 Otello, 늘쌀보리 및 흰쌀보리 등 3품종으로 2000년 4월에 교배하여 6월에 채종하였다. 그리고 2001년 F1세대를 거쳐 2002년 F2세대에서 2007년 F7세대까지 12계통을 분리계대육성하여 homo화 시켜 흑쌀보리의 육성의 교배친과 이들 F7세대의 교배조합에 대한 유전력과 상관관계에 대한 연구 결과는 다음과 같다.교배친에서는 유전분산이 환경분산에 비하여 높았으며 유전분산은 천립중이 15.2658로 가장 높았던 반면, 망장이 0.0833으로 가장 낮았고 모든 형질에서 고도의 유의성을 나타내었다. 유전력은 β-glucan이 99.99%로 가장 높았으며 망장을 제외한 각 조사형질에서 95%이상의 높은 수치를 보였다. F7세대의 교배조합에서도 모든 형질에서 고도의 유의성을 나타내었으며, 유전분산에서 천립중이 6.9148로 가장 높았다. 교배친에서 β-glucan 함량과 천립중간에, β-glucan 함량과 망장간에서는 유의한 정의 상관이었고, 수장과 β-glucan 함량간에는 부의 상관을 보였다. F7 세대의 교배조합에서는 천립중과 β-glucan 함량간에서 정의 상관을 보였으나 그 수치는 다소 낮았다.
The limited use of barley for food is mostly due to undesirable color and unfamiliar flavor of barley based food products. So, preventing the browning is very important in order to increase the consumption of barley. ‘Yeongbaekchal’is a new naked waxy barley cultivar which is not get discolored. It was developed by the National Institute of Crop Science, RDA in 2013. It was derived from the cross between‘Radiant’which is a proanthocyanidin free and ‘Saechalssal’ which has characteristics of winter hardness, lodging tolerance, waxy endosperm and good cooking. The initial cross was done in 2006 and the selected line, ‘Iksan116’(HB16953-B-B-7), had no proanthocyanidin and showed good quality characteristics under yield trial test from 2012 to 2013. Heading and maturing date of ‘Yeongbaekchal’ were 3days late than those of ‘Saechalssal’. It had 90 cm of culm length and 5.0 cm of spike length. It showed 840 spikes per m2, 63 grains per spike, 25.1 g of 1,000-grain weight, and 831 g of test weight. ‘Yeongbaekchal' showed better resistance to BaYMV (Barley yellow mosaic virus) and similar water absorption rate and expansion rate with the check cultivar. Its average yield of the pearled grain in the regional yield trial was 3.42 MT/ha in paddy field, which were 5% lower than that of the check cultivar. It showed a little discoloration during the storage after cooked.
Current consumers have interest in nutrition and health. So, the interest in barley for other food and industrial applications is increasing. Noodles, which have been consumed largely in korea, are usually made from wheat flour, salt and appropriate amount of water. A New Six-rowed Naked Barley Cultivar “Dahan” was developed in 2012, have high winter hardness, lodging tolerance and good noodle property. It was crossed in 2001, and an elit line was selected in 2010 and designated as ‘Iksan 106’. It showed good agronomic performance in the regional yield trials (RYT) from 2010 to 2012. The average heading and maturing dates of “Dahan” were April 29 and June 3 in paddy field, which were two day earlier than those of the check cultivar ‘Saessalbori’ respectively. The new cultivar, “Dahan”, had 92cm of culm length and 3.9cm of spike length. It showed 625 spikes per m2, 54 grains per spike, 31.3 g of 1,000-grain weight, and 815 g of test weight. “Dahan” showed better resistance to BaYMV (Barley yellow mosaic virus) than those of the check cultivar. Its average yield of the pearled grain in the regional yield trial was 4.32 MT/ha in paddy field, which were 16% higher than those of the check cultivar. It showed higher breakdown and setback than those of the check cultivar. Texture properties of noodle was better in hardness, adhesiveness, chewiness. This cultivar would be suitable for the industrial application as noodle and bread.
A New six-rowed Naked Barley Cultivar “Nurichal” with good coking quality was developed by cross with HB88154-B-25-1 line and Hinchalssalbory by the Winter Cereal and Forage Crop Research Div. NICS, RDA in 2010. HB15305-B-B-31-2 was selected in 2006 and designated as ‘Iksan 95’. It showed good agronomic performance in the regional yield trials (RYT) from 2008 to 2010 and was released with the name of ‘Nurichal’ possessing good water absorption and expansion. The average heading and maturing dates of ‘Nurichal’ were April 24 and May 30 in paddy field, which were two day and one day later than those of the check cultivar ‘Saechalssalbori’ respectively. The new cultivar, ‘Nurichal’, had 82cm of culm length that was 5cm shorter than that of ‘Saechalssalbori’, and 4.0cm of spike length. It showed 774 spikes per m2, 63 grains per spike, 25.0 g of 1,000-grain weight, and 793 g of test weight. ‘Nurichal’ showed a little weaker winter hardiness than those of the check cultivar, ‘Saechalssalbori’ and showed similar lodging resistance with check cultivar. It sowed stronger resistance to barley yellow mosaic virus (BaYMV) compare to check cultivar. Grain shape was small and round. Protein content was 10.3% and β-glucan content was 6.8%. Its average yield of the pearled grain in the regional yield trial was 3.84 MT/ha in paddy field, which were higher 2% than those of the check cultivar. Its water absorption was similar and expansion was higher than ‘Saechalssalbori’. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.
A New Six-rowed Naked Barley Cultivar “Ganghocheong” with High anthocyanin content and green aleurone the cross between Iri26 and Sagukgwa67 by the National Institute of Crop Science, RDA in 2009. An elite line HB14190-B-61-3-2-3-3-5-1 was selected in 2004 and designated as ‘Iksan 92’. It showed good agronomic performance in the regional yield trials (RYT) from 2007 to 2009 and was released with the name of “Ganghocheong” possessing high anthocyanin content and green lemma non-waxy endosperm. The average heading and maturing dates of “Ganghocheong” were April 20 and May 29 in paddy field, which were two days earlier and same than those of the check cultivar ‘Saessalbori’ respectively. The new cultivar, “Ganghocheong”, had 81cm of culm length and 6.0cm of spike length. It showed 702 spikes per m2, 60 grains per spike, 30.0 g of 1,000-grain weight, and 794 g of test weight. “Ganghocheong” showed better resistance to BaYMV (Barley yellow mosaic virus) than those of the check cultivar, ‘Saessalbori’. Its average yield of the pearled grain in the regional yield trial was 4.02 MT/ha in paddy field, which were 1% lower than that of the check cultivar, respectively. Anthocyanin content was 122.1㎍/g that was higher 2 times than that of zasoojeongchalssalbori. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.
신품종 "진주찰" 쌀보리는 취반 후 백도가 좋고, 베타글루칸 함량이 높은 소립, 내재해, 양질, 다수성 쌀보리 신품종이다. 1996년에 호남농업연구소에서 도복과 내한성이 강하고 찰성으로 립백도와 정맥율이 높으며 취반특성이 우수한 특성을 지닌 "진미찹쌀보리"와 "수원333호"를 인공교배한 후 계통육종법으로 육성하였다. 2003년부터 2년간 생산력검정시험을 실시한 결과, 수량성이 높고 도복이 강한 우수 계통으로 판명되어 "익산79호"로 계통명을 부여, 20
‘Zasoojeongchal’ is a new colored naked barley cultivar developed from the cross between HB83072〔Tapgolbori / HB 78100-B-17-3-2〕, a disease resistant and good quality line and ‘Dokusima mochi hadaka’, colored naked cultivar with waxy endosperm by the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. An elite line CNB7-13-37-2-5-3, was selected in 2002 and designated as ‘Iksan 81’. It showed good agronomic performance in the regional yield trials (RYT) from 2005 to 2006 and was released with the name of ‘Zasoojeongchal’ possessing purple colored waxy endosperm. The average heading and maturing dates of ‘Zasoojeongchal’ were May 2 and Jun. 7, which were two and five days later than those of the check cultivar ‘Saechalssalbori’ respectively. The new cultivar, ‘Zasoojeongchal’, had 75cm of culm length that was 4cm shorter than that of ‘Saechalssalbori’, and 5.4 cm of spike length. It showed 607 spikes per m2, 63 grains per spike, 27.2 g of 1,000-grain weight, and 771 g of test weight. ‘Zasoojeongchal’ showed weaker winter hardiness, but better resistance to powdery mildew and BaYMV (Barley yellow mosaic virus) than those of the check cultivar, ‘Saechalssalbori’. It showed higher ß-glucan content, anthocyanin content, water absorption rate and expansion rate than those of the check cultivar, ‘Saechalssalbori’. Its average yield of the pearled grain in the regional yield trial was 2.67 MT/ha in upland, and 3.49 MT/ha in paddy field, which were 26% and 6% lower than those of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.
In general, since partial pollen is derived to sporophyte, anther culture efficiency is low, and practical application that is introduced in actuality breeding technology is not many. This study was carried out to improve regeneration of green plants through some culture environments contol in anther culture of naked brley. Among the factors related with plant regeneration, medium was effective in component containing L-glutamine 256 mg/L, L-proline 250 mg/L, IAA 1 mg/L and BAP 2 mg/L controlled in addition sucrose 30 g/L or maltose and sucrose mixed each 30 g/L to increase both plant regeneration and green plant regeneration rate. Also, adequate content of CuSO4 was the best at 1.25 mg/L (fifty-fold), it was tendency to decrease albino production rate. Starvation was effective at 30℃, 7 days in case of Saessalbori for plant regeneration and Dooweonchapssalbori at 30℃, 10 days with increasing green plant regeneration against albino. After plant regeneration, under acclimation by hydroponics, roots and shoots were well developed at 20℃, light control as 2,000~5,000 Lux and photoperiodic reaction by 14/10 as dark/light in the early growth stage. In acclimation method, plants acclimated in the modified Yoshida solution filled-vermiculite in GP pot is superior (100%), in which was controlled by temperature 20℃, pH 6.0 and relative humid 90% over, especially, after transplanting in pot growth of root, sheath and leaf are more active in 20℃ and 5,000 Lux control. For Vernalization, plants derived from anther culture of F1 naked barley was different from their parents with normal heading plant even about 50% in the F1 hybrids whose vernalization was strong, whereas the rest of plants derived from anther culture formed rosette, showing that normal growth were impossible.
A new six-rowed naked waxy barley variety, ‘Saehanchal’, was developed by the barley breeding team of the National Institute of Crop Science (NICS), R.D.A. This variety was derived from a cross between ‘SB7803G-BC6-B-B-47-2’ and ‘Suwon262’ in 1989. The fi
The six rowed and naked barley cultivar, “Cheongho” (Hordeum vulgare L.), showing earliness, good cooking quality, resistance to powdery mildew and barley yellow mosaic virus disease (BaYMV), winter hardiness, lodging tolerance, and high yield in paddy fi