Button mushroom(Agaricus bisporus) are cultivated on wheat straw or rice straw based compost. In this study different compost formular, sawdust as carbon source and chicken manure as nitrogen source were used for button mushroom cultivation. Two steps of sufficient composting process was required to be successful in the cultivation. 1) the substrates are needed to be mixed periodically under the aerobic condition in the phase I process. 2) the temperature is need to be maintained between 55 and 65? for more than three days in the phase II process. Results showed that button mushroom was possible to grow normally with sawdust based compost. However the mushroom yield was less than that of the conventional compost formular(rice straw or wheat straw). The new compost formular developed from this study may be useful but further research may need to improve button mushroom cultivation more efficiently.

FRP composites bridge deck has advantages of structural characteristics and rapid construction in the replacements of the deteriorated bridge deck. Although FRP composites have many advantages, the application in the bridge design has been retarded so far due to the lack of design guidelines. In this paper, the design example for the FRP decked concrete composite girder bridges is presented to verify the proposed design method. The design of connection in the design example is the flexible hybrid shear connection included steel reinforcements and FRP tubes. Finally, this paper may be design guideline for FRP decked concrete composite girder bridges required the composite action.

환경과 개발이 조화된 한반도 국토 종합개발 계획 수립을 위해서는 환경계획에 활용할 수 있는 환경공간정보가 필요하다. 하지만 기 구축되어 있는 북한지역의 환경공간정보는 매우 한정적이다. 기존 환경부의 대분류 토지피복지도가 있으나, 대분류 토지피복지도는 국토의 토지피복분류를 7개 항목으로만 분류체계가 구분되어 있고, 공간 해상도 또한 떨어져 정책적으로 활용할 수 있는 분야가 제한적이다. 따라서 본 연구에서는 접근불능지역을 대상으로 원격탐사 기술을 적용하여 신규 구축이 가능한 토지피복지도가 북한 지역의 환경공간정보로 타당하 다고 판단하였다. 그리고 기존 대분류 토지피복지도보다 공간 및 속성 해상도가 높아 다양한 분야에서 활용할 수 있는 중분류 토지피복지도를 구축하고자 하였다. 이를 위해 환경, 도시, 산림, 농업 분야의 토지피복지도 관련 구축 현황을 살펴보았으며, 각각의 분야에서 이용하고 있는 분류체계를 기반으로 중분류 토지피복지도의 구축 가능성과 활용성을 고려하여, 기존 환경부 중분류 토지피복지도의 22개 분류체계에서 신규 16개 분류체계로 변경하여 각각의 분류체계의 구축방안을 제시하였다. 그리고 개성지역을 시범지역으로 선정하고, 각각의 분류체계별 구축방안을 시범 구축하였다. 이러한 북한지역의 토지피복지도의 시범구축 결과 분류체계의 항목별 전체 분류정확도는 95.35%로 산정되어, 북한지역의 중분류 토지피복지도 구축에 적용가능하다 판단하였다.

Building hardware joints are welded in most cases, which have risks of fire and explosion. Besides, the secondary damage of the destruction of the welded parts can be caused by the horizontal displacement of the structure due to earthquake or wind load. This paper compared the horizontal displacement following abilities of welded building hardware and non-welded building hardware. To do this, We conducted actual formation shake table test, and checked on the horizontal displacement following ability of structure by comparing their responses to earthquake load. We made the 2m-high framework to examine the responses of the actually constructed building hardwares, and analyzed the displacement responses of the welded-typed, non-welded-typed, and cruciform bracket building hardwares. We conducted the test by increasing acceleration rate until displacement reached 40mm corresponding to allowable relative story displacement II. The result of the test showed that the building hardware using welding work made cracking and breakage on welded connections of welded building hardware, but non-welded building hardware with no use of welding work and cruciform bracket building hardware make no problem, and that non-welded building hardware is superior to that of the welded building hardware in the horizontal displacement following ability due to earthquake or wind load.

산느타리버섯(Pleurotus pulmonarius (Fr.) Quelet)은 담자균문, 주름버섯목, 느타리과, 느타리속에 속하며 봄부 터 가을에 걸쳐 활엽수의 죽은 나무 또는 떨어진 가지에 군생하거나 단생하는 백색부후균이다. 맛과 향이 좋 고 씹는 질감이 뛰어나며, 항염증, 항콜레스테롤, 항고지혈증의 기능성을 가지고 있으며, 국내 육성품종으로 는 ‘호산’, ‘강산’, ‘향산’, ‘화산’, ‘자산’ 등 5종이 개발되어 있다. 본 시험에서 가격이 저렴한 맥주박과 건비 지를 이용하여 생육특성을 조사한 결과, 두 품종 모두 I 배지 수량이 가장 높았다. 맥주박과 건비지가 혼합된 II, III의 대부분의 배지에서 배양이 완료되기 전에 측면 발이 되고, 병뚜껑 틈새로 버섯이 발생되어 제거하여 주었는데 이 부분에서 수량이 감소되는 원인으로 보여진다.

산느타리버섯(Pleurotus pulmonarius (Fr.) Quelet)은 담자균문, 주름버섯목, 느타리과, 느타리속에 속하며 봄부 터 가을에 걸쳐 활엽수의 죽은 나무 또는 떨어진 가지에 군생하거나 단생하는 백색부후균이다. 맛과 향이 좋고 씹는 질감이 뛰어나며, 항염증, 항콜레스테롤, 항고지혈증의 기능성을 가지고 있으며, 국내 육성품종으로 는 호산, 강산, 향산, 화산, 자산 등 5종이 개발되어 있다. 현재 버섯의 가격하락과 인건비 상승 그리고 배지 의 가격이 높아짐에 따라 농가의 어려움이 더해가고 있어 기존 배지재료를 대체할 수 있는 염가재료가 시급 하다. 본 시험에서는 구하기 쉽고, 가격이 저렴한 맥주박과 왕겨를 이용하여 품종별, 배지혼합비율별에 따른 수량 등 생육특성을 검토하였다. 맥주박의 경우 배합비율은 대조구를 IV로하여 I, II, III에 면실피, 면실박을 대체 재료로 맥주박을 첨가하여 조사한 결과, 호산은 IV 배지가 115.5g, 자산의 경우 I 배지가 103.1g으로 수 량이 가장 높았다. 미루나무톱밥 대체 재료로 왕겨를 첨가하여 조사한 결과, 호산 품종의 경우 왕겨를 혼합 하지 않은 IV의 수량이 높은 반면 자산 품종은 왕겨가 15% 첨가된 I 배지의 수량이 가장 높은 것으로 나타 났다.

The biocidal activity of Cu(II) is greatly enhanced by the addition of H2O2 or hydroxylamine (HA), which accelerates the production of cytotoxic Cu(I) and generates a reactive oxidant (cupryl ion, Cu(III)) capable of exerting oxidative cell damages. The bimetallic Fe-Cu nanoparticles also exhibit excellent biocidal activity in the similar biocidal mechanism. These copper-based hybrid disinfection systems were assessed for their potential to inactivate surrogate bacteria and viruses. In addition, combinations of Cu(II) with HA and H2O2 (i.e., Cu(II)/HA, Cu(II)/H2O2, and Cu(II)/HA/H2O2 systems) were tested for the control of bacterial biofilms on reverse osmosis (RO) membranes. The detailed mechanisms for the cell inactivation by different copper-based disinfection systems were discussed.

본 연구에서는 한국 멸종위기식물인 분홍장구채의 보전 및 인공재배를 위해 식물공장 내 인공적인 광 조건에서 일어나는 생리와 개화반응 특성을 알아보았다. 이를 위해 분홍장구채를 식물공장의 각 챔버에 적색광, 청색광, 백색광, 적색+원적색 혼합광, 적색+청색 혼합광 그리고 적색+청색+백색 혼합광 하에서 재배하여 광합성률, 증산률, 기공전도도, 수분이용효율, 엽록소함량, 최소엽록소형광, 최대엽록소형광, 광계II의 광화학적 효율 그리고 잎과 꽃 수를 측정하였다. 그 결과 적색 단일광과 적색+원적색 혼합광에서 분홍장구채는 가장 많이 개화하였으며, 이 광조건에서는 광합성률, 증산률 그리고 기공전도도는 상대적으로 다른 조건보다 높았으나 수분이용효율은 차이가 없었으며, 엽록소함량은 적었 다. 특히 최소 및 최대 엽록소형광과 광계II의 광화학적 효율은 적색 단일광 조건에서 다른 광 조건보다 낮았다. 이러한 결과는 분홍장구채의 꽃의 생산을 위해서는 적색 단일광이나 적색+원적색의 인공광 조건에서 재배하는 것이 효율적이 라는 것을 의미한다.

이 연구에서는 최근 관련 유적이 다수 조사된 춘천분지 일원 청동기~초기 철기시대 유적군의 획기와 편년 체계를 구축하였다. 춘천분지 청동기-초기 철기시대 I단계는 타원형 석상위석식노지 방형 주거지·이중구연거치문토기· 사장경호·무경식석촉·장방형어망추·장방형석도, II단계는 위석식노지 장방형 중심 주거지·각종 돌대 문토기·이중구연토기·구순각목토기·복합문토기·무경식석촉·장방형어망추·장방형석도, III단계는 (퇴화형 위석식노지+)수혈식노지 세장방형 중심 주거지·천발형토기·발형토기·호형토기·공열토기·적 색마연소호·무경식석촉·이단경식석촉·일단경식석촉·이단병식석검·타원형어망추·제형석도·주형석 도, IV단계는 수혈식노지 장방형 중심 주거지·기존 토기 외 다양한 적색마연토기(발형토기, 호, 대부소 호)·무경식석촉·일단경식석촉·타원형어망추·이(일)단병식석검·주상편인석부·주형석도·어형석도 등이 공반하는 단계이다. V단계는 전형적인 천전리식 주거지·무경식석촉·일단경식석촉·일체형석촉· 원통형어망추·일단병식석검·유경식석검·유구석부·주형석도·어형석도, VI단계는 소형 수혈식노지 방 형 중심 주거지·순수 무문토기 중심 토기·일체형석촉·일단병식석검·검병두식·유구석부·주형석도, VII단계는 소형 수혈식노지 말각방형 주거지·점토대토기·두형토기·흑색마연토기·조합식우각형파수· 우각형파수·봉상파수·삼각만입석촉·검병두식·유구석부·주형석도 등이 공반하는 단계이다. 각 단계 의 시간적 범위는 기원전 12~11세기(I단계), 기원전 11~10세기(II단계), 기원전 9~8세기(III단계), 기원전 8~7세기(IV단계), 기원전 6~5세기(V단계), 기원전 4~3세기(VI단계), 기원전 3세기 전반~1세기 중엽(VII단 계)이다.

The purpose of this study was to investigate the effects of strengthening and stretching exercises on forward head angle and static·dynamic balance ability. 21 adults with the forward head posture were participated in this study. Subjects were randomly assigned to strengthening exercise group I(n=11) and stretching exercise group II (n=10) respectively. Each group were underwent six intervention over the period of 2 weeks, each session lasting 30 minutes. During a preliminary examination, forward head angle and static∙dynamic balance were measured, and a post examination was conducted that involved same procedure as preliminary examination. There were significant differences in forward head angle after exercise in both groups. In the strengthening group, center of gravity total sway distances was significantly changed under all conditions. In the stretching group, center of gravity total sway distances was significantly changed when subjects had their eyes open while standing on an foam surface. There were significant changes in center of gravity sway velocities in the strengthening group under all conditions, and the same was true in the stretching group only when the subjects had their eyes open while standing on an foam surface. In the comparison between groups, eyes closed while standing on a firm surface was significantly different. There was a significant difference in dynamic balance of the stretching group when the subjects tilted their bodies forward, and there was a statistically significant difference between groups under the same condition. The results of this study suggest that forward head posture can be corrected through therapeutic exercise, and muscle strengthening exercise more effective in improving static balance of forward head posture more than stretching exercise.

흑산 니질대에서 채취한 코어 퇴적물 HMB-103으로부터 규조를 분석하여 3개의 규조 군집대를 설정하였고, 산 출된 한랭수종과 온난수종, 염분도에 의해서 고환경을 해석하였다. 규조는 총 41속 76종이 감정되었으며 규조 개체수 농도는 0.1-15.4×104 g−1 의 범위에 해당된다. 연구 결과, 약 45,000 yr B.P.경까지의 규조 군집대 I에서는 한랭수종의 산 출이 많은 것으로 보아 한국연안류의 영향을 주로 받았으며, 14,000부터 11,646 yr B.P경까지의 규조 군집대 II는 규조 의 산출이 거의 없는 추운 시기(Younger Dryas)로 생각된다. 그러나, 11,646 yr B.P부터 홀로세까지의 규조 군집대 III 시기에는 해수면이 상승하면서 황해 난류의 영향을 더 많이 받았다.

A meningioma is the second most common primary intracranial tumor of the central nervous system. One critical obstacle in meningioma research and preclinical development of novel therapeutic agents is a relative lack of suitable preclinical in vitro and in vivo model systems. In the current study, we assessed the proliferative characteristics of patient derived five primary meningioma cancer cell lines (WHO grade I and II) from brain tumor lesions. All of the meningioma cell lines showed homogenous expression of meningioma marker, VIMENTIN. The GTG-banding analysis determined the existence of different patterns of chromosomal abnormalities in representative cancer cell lines. The almost of the meningioma cell lines showed homogeneously spindle shaped cells, except for M160425 which have two prominent cell morphologies, spindle and round. Population-doubling (PD) was measured for every passage. The M160425 cell line had significantly longer PD time (39.8 ± 0.9 h, P<0.05) than the other meningioma cell lines. Consistent with the PD time, we confirmed that mRNA expression of Ki67, the conventional proliferation marker, was significantly lower in M160425 cell line compared to the other cell lines. The correlation between the PD time and the abundance of Ki67 in the meningioma derived cell lines was negative, indicating higher Ki67 abundance and a shorter PD time. The patient derived meningioma cancer cell lines established in this study might contribute to understanding cancer biology and help the success in the clinic by explaining the slightly different clinical behavior among the patients.

Crocin is a carotenoid that may protect cells against oxidative stress by scavenging free radicals particularly superoxide anions. It has been reported that oocyte maturation is influenced by the free radicals generated during in vitro culture (IVC) process. The objective of study was to examine the effect of crocin in in vitro maturation (IVM) medium as an antioxidant on oocyte maturation and embryonic development after parthenogenesis (PA). Cumulus-oocyte complexes (COCs) were collected from ovaries of prepubertal gilts. The basic medium for IVM was medium-199 containing 10% pig follicular fluid, cysteine, pyruvate, epidermal growth factor, kanamycin, insulin, and hormones. Oocytes were treated for 44 hours with crocin at 0, 25, 50, and 100 μg/ml during IVM. Oocytes reached the metaphase II stage were induced for PA and cultured for 7 days in porcine zygote medium-3. Nuclear maturation of oocytes was not influenced by various concentrations of crocin (89.0, 87.3, 84.3, and 94.1% for control, 25, 50, and 100 μg/ml crocin, respectively). IVM oocytes treated with 50 μg/ml crocin showed a higher (P<0.05) intraoocyte glutathione (GSH) contents than untreated oocytes (1.00 vs. 1.29 pixels/oocyte). Blastocyst formation of PA embryos treated with 50 (42.9%) and 100 μg/ml crocin (43.8%) was significantly higher (P<0.05) than oocytes treated with 25 μg/ml crocin (30.5%) but not different from that (35.2%) of untreated oocytes. In summary, crocin increases cytoplasmic maturation in terms of intraoocyte GSH content which may be beneficial for later embryonic development by protecting from harmful effect of reactive oxygen species. Further studies are needed to determine whether the beneficial effect of crocin treatment during IVC would be shown in embryonic development after in vitro fertilization and somatic cell nuclear transfer.

Oocytes from small antral follicles (< 3 mm in diameter; SAFO) show lower developmental competence compared to those from medium antral follicles (3-8 mm in diameter; MAFO) in pigs. This study was designed to evaluate the effect of various macromolecules such as fetal bovine serum (FBS), porcine follicular fluid (PFF), bovine serum albumin (BSA) and polyvinyl alcohol (PVA) in in vitro growth (IVG) medium on oocyte growth, maturation, and embryonic development after parthenogenesis (PA). The base medium for IVG was α-MEM supplemented with dibutyryl cyclic AMP, pyruvate, kanamycin, hormone. This medium was further supplemented with 10% FBS, 10% PFF, 0.4% BSA, or 0.1% PVA. The in vitro maturation (IVM) medium was medium-199 supplemented with 10% PFF, cysteine, pyruvate, epidermal growth factor, kanamycin, insulin, and hormones. SAFO were cultured for 2 days for IVG and then cultured for 44 h for IVM. After IVG, the mean diameter of SAFO treated with FBS, PVA, and no IVG-MAFO (114.1, 113.0, and 114.8 μm, respectively) was significantly larger (P<0.01) than that of no IVG-SAF (111.8 μm). Oocyte diameter after IVM was greater (P<0.01) in SAFO treated with FBS, BSA and PVA (112.8, 112.9 and 112.6 μm, respectively) than other groups (110.4, 109.6, and 109.8 μm for no IVG-MAFO, no IVG-SAFO and PFF, respectively). Intraoocyte GSH content was not influenced by the macromolecules in IVG medium (0.92, 0.93, 1.05, and 1.12 pixels/oocyte for FBS, PFF, BSA and PVA, respectively). The proportion of oocytes reached the metaphase II stage was higher in PFF (73.6%) than in BSA (43.5%) and PVA (53.7%) but not different from that of FBS treatment (61.5%). The cumulus expansion score of oocytes after IVG was significantly influenced (P<0.01) by the macromolecules (2.94, 2.24, 1.84, and 1.38 for PFF, FBS, PVA, and BSA treatments, respectively). Blastocyst formation of PA oocytes that were treated with FBS (51.8%), PFF (50.4%), and PVA (45.2%) during IVG was higher (P<0.05) than that of BSA-treated oocytes (20.6%) but was not significantly different from that (54.8%) of no IVG-MAFO oocytes. Our results demonstrated that growth, maturation, and embryonic development of SAFO are greatly influenced by macromolecules in IVG medium and that PFF or FBS can be replaced with a chemically defined synthetic macromolecule PVA.

In mature oocytes, maturation promoting factor (MPF) activity is playing important roles in arrest at M-phase and its continuous phenomenon, oocyte aging. In most mammals, metaphase II oocytes show high MPF activity and have been used as ooplasts in somatic cell nuclear transfer (SCNT). Caffeine has been found to regulate MPF activity in mammalian oocytes. Caffeine inhibits p34cdc2 phosphorylation and increases MPF activity. The present study investigated the effects of caffeine treatment during last 4 hours of in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenesis (PA) and SCNT. The IVM medium was medium-199, 10% (v/v) PFF, cysteine, pyruvate, epidermal growth factor, kanamycin, insulin, and hormones. Immature oocytes were matured in IVM medium without or with 2.5 mM caffeine during the last 4 hours of IVM. The in vitro culture medium for embryonic development was porcine zygote medium-3 containing 0.3% (w/v) bovine serum albumin. Nuclear maturation (83.6–87.2%) and intraoocyte glutathione contents (0.9–1.0 pixels/oocyte) of oocytes were not influenced by the caffeine treatment. The membrane fusion of cell-cytoplast couplets (75.5–76.5%) and cleavage (85.4–86.2%) were also not altered by the caffeine treatment. However, caffeine-treated oocytes showed higher (P<0.05) blastocyst formation after SCNT (47.5 vs. 34.3%) than untreated oocytes. Our results demonstrate that caffeine treatment during last 4 hour of IVM improves the developmental competence of SCNT embryos probably by influencing MPF activity.

Crocin is a carotenoid that may protect cells against oxidative stress by scavenging free radicals particularly superoxide anions. It has been reported that oocyte maturation is influenced by the free radicals generated during in vitro culture (IVC) process. The objective of study was to examine the effect of crocin in in vitro maturation (IVM) medium as an antioxidant on oocyte maturation and embryonic development after parthenogenesis (PA). Cumulus-oocyte complexes (COCs) were collected from ovaries of prepubertal gilts. The basic medium for IVM was medium-199 containing 10% pig follicular fluid, cysteine, pyruvate, epidermal growth factor, kanamycin, insulin, and hormones. Oocytes were treated for 44 hours with crocin at 0, 25, 50, and 100 μg/ml during IVM. Oocytes reached the metaphase II stage were induced for PA and cultured for 7 days in porcine zygote medium-3. Nuclear maturation of oocytes was not influenced by various concentrations of crocin (89.0, 87.3, 84.3, and 94.1% for control, 25, 50, and 100 μg/ml crocin, respectively). IVM oocytes treated with 50 μg/ml crocin showed a higher (P<0.05) intraoocyte glutathione (GSH) contents than untreated oocytes (1.00 vs. 1.29 pixels/oocyte). Blastocyst formation of PA embryos treated with 50 (42.9%) and 100 μg/ml crocin (43.8%) was significantly higher (P<0.05) than oocytes treated with 25 μg/ml crocin (30.5%) but not different from that (35.2%) of untreated oocytes. In summary, crocin increases cytoplasmic maturation in terms of intraoocyte GSH content which may be beneficial for later embryonic development by protecting from harmful effect of reactive oxygen species. Further studies are needed to determine whether the beneficial effect of crocin treatment during IVC would be shown in embryonic development after in vitro fertilization and somatic cell nuclear transfer.

Mitochondrial dysfunction is found in oocytes and transmitted to the offspring due to maternal obesity. This is curable by endoplasmic reticulum (ER) stress inhibitors such as salubrinal (SAL). Recently pigs are considered as a model animal for biomedical research due to its physiological similarity with human. Pig oocytes have shown ER stress mostly in metaphase II stage. ER stress is hindering the in vitro embryo production (IVP). This study investigated the effect of ER stress inhibition by using SAL during 44 h of in vitro maturation (IVM) of oocytes at 1, 10, 50 and 100 nM concentrations. Firstly, we defined the concentration of SAL during IVM of pig oocytes. SAL at 10 nM showed higher (44.2 to 55.6%, P<P0.05) development competence to the blastocyst state than control and other concentrations after parthenogenetic activation (PA). Secondly, we sorted out the time-dependent treatment at 10 nM of SAL for IVM of oocytes. It revealed that treatment with SAL during 22 to 44 h and 0 to 44 h of IVM improved PA embryonic development significantly (40.5, 51.7 and 60.2% for control, 22 to 44 h and 0 to 44 h of IVM, respectively, P<0.05). Glutathione (GSH) level is an indicator of cytoplasmic maturation of oocytes. Reactive oxygen species (ROS) have a harmful effect on development competence of oocytes. For this, we determined the intraoocyte levels of GSH and ROS after 44 h of IVM. It was found that SAL increased intraoocyte GSH level and also decrease ROS level (P<0.05). Finally, we performed somatic cell nuclear transfer (SCNT) after treating oocytes with 10 nM SAL during IVM. SAL treatment significantly improved blastocyst formation of SCNT embryos compared to control (24.7 vs. 39.6%, P<0.05). Our results indicate that treatment of pig oocytes with ER stress inhibitor SAL during IVM improves preimplantation development cloned pig embryos by influencing cytoplasmic maturation in terms of increased GSH content and decreased ROS level in IVM pig oocytes.