To investigate the change of vaginal epithelial cell in estrus-induced Shih-tzu bitch, estrus was induced by PMSG (50 IU/kg, for 10 days) and hCG (1,000 IU, on Day 10) in 12 anestrus Shih-tzu bitches. Day-changes of vaginal epithelial cells during the whole period of hormone treatment were investigated in each experimental bitches. The first day of vulvar bleeding and the first day of male acceptant was on days (), and days after the first PMSG treatment. The duration of proestrus and estrus was days, and days. Characteristic features of vaginal cytology during the estrous cycle were the high proportion of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell and anuclear cell in estrus, and parabasal cell, small and large intermediate cell, and leukocyte in diestrus, respectively. When it was timed from the first day of PMSG administration (Day 0), the cornification index (CI) was the high proportion in proestrus and estrus. The CI peak was maintained above 80% between Day 11~14 (4 days) and CI showed a peak at Day 12. These results indicated that the 12 Shih-tzu bitches showed positive estrus induction by vaginal smear test and observation of clinical estrus sign.

The quality of baked Yackwa with rice bran oil varied with dipping temperature (80 and 25℃) and dipping time (5, 10, and 15 minutes at 80℃, and 60, 90, and 120 minutes at 25℃). The moisture content and hardness of baked Yackwa increased and decreased, respectively, with dipping time. The expansion ratio increased with time when baked Yackwa was dipped at 80℃, but decreased with time when dipped at 25℃. Flavor and color were optimal for product dipped at 80℃ for 15 minutes followed those dipped at 25℃ for 120 minutes; the dipping conditions produced no significant difference in the degree of expension, aroma, texture, and overall quality. Baked Yackwa showed better results than fried Yackwa in sensory evaluation.

본 연구는 주요 냉장식품(두부, 묵, 어육가공품(어묵), 생면, 김초밥, 샐러드)의 온도 관리실태를 조사하여 문제점을 파악하고 국내 유통업소의 냉장·냉동진열 판매 제품의 관리온도 개선 및 관리방안을 제시하고자 하였다. 주요 냉장고 내 위치별 온도는 보편적으로 아래(7.5 ℃)가 높고 윗부분(6.4 ℃)이 낮았으며, 앞쪽(8.9 ℃)이 높고 뒤쪽(7.5 ℃)이 낮은 것으로 조사되었다. 모든 냉장식품의 평균 냉장고 보관 온도는 7 ℃(최대 22.9 ℃, 최저 -2 ℃)였으며, 대형할인매장이 6.5 ℃, 백화점이 7.5 ℃, 편의점이 7.6 ℃ 였다. 모든 냉장식품의 평균 표면온도는 8.2 ℃(최대 19 ℃, 최저 -0.4 ℃)였으며, 대형할인매장이 8.1 ℃, 백화점이 8.5 ℃, 편의점이 7.6 ℃으로 조사되었다. 식품표면과 중심온도는 식품별 차이가 있으며 최대 2.3 ℃ 차이로 나타났다. 두부(표면온도 6.1~10.6 ℃, 중심온도 4~11.1 ℃), 어묵(6~12.3 ℃, 6.5~13.6 ℃), 김밥(2.3~18.2 ℃, 2~17.3 ℃), 샐러드류(4.6~12.2 ℃, 4.1~10.4 ℃), 생면류(3.4~12.6 ℃, 3.8~10.3 ℃), 묵류(7.1~19 ℃, 8.3~17.1 ℃)의 다섯 가지 식품별로 차이를 보였다. 냉장고 표시온도와 식품표면 온도 항목간의 유의적 차이가 있는 것으로 나타났다(P<0.01). 냉장고 표시 온도는 김밥 1곳(100%)에서 규정 온도인 10 ℃를 초과했지만, 식품표면 온도에서는 두부 9곳(17%), 어묵 6곳(11.3%), 묵 10곳(18.9%), 생면 11곳(20.8%), 김밥 13곳(24.5%), 샐러드 4곳(7.5%)이 초과한 것으로 나타났다. 결론적으로 우리나라 냉장보관 식품 중 표면온도와 10 ℃를 초과하는 경우가 많아 냉장보관식품의 온도관리가 철저히 이루어져야 할 것으로 판단된다.

This study addresses the development of a soy hamburger patty containing enzyme-treated textured soy protein (TSP) as a meat analogue. In order to reduce the beany flavor and enhance the texture, TSP was treated with 0.3% Flavourzyme for 5, 10, 20, or 30 min. The degree of hydrolysis and the water holding capacity of the TSP increased with increasing hydrolysis time. The oil binding capacity of the TSP also increased with increasing hydrolysis time, approaching the maximal value, 175.82%, at 30 min, whereas that of pork scored with the lowest value of 128.67%. The volume of pork was reduced to 81.5% as the result of heat treatment, whereas that of the TSP increased to 140.57%. The values of 'L', 'b', and 'δE' differed significantly (p〈0.001) with heat treatment, but the 'a' values did not differ significantly. With regard to texture, the hardness values were highest in the pork hamburger patty (PHP), and were lowest in the soy hamburger patty (SHP) containing untreated TSP. The hardness of the SHP containing TSP treated for 20 min did not differ significantly from that of the PHP. The cohesiveness and gumminess of the SHP treated for 20 min were highest, whereas those treated for 10 min were the lowest. The gumminess of the SHP treated for 20 min did not differ significantly from that of pork. The chewiness of the PHP was the highest, whereas that of the SHP treated for 5 min was the lowest. In our sensory evaluation, PHP evidenced the highest scores, followed by the SHP treated for 30 min, as color, texture, beany flavor, and overall quality all improved as the consequence of increasing enzyme treatment duration. In conclusion, it is believed that SHP has great potential as a substitute for meat, in that the flavor, texture, and beany flavor of SHP did not differ significantly from those of PHP.

The development of soy cutlets containing textured soy protein (TSP) as a meat analog was studied. In order to decrease the beany flavor and to increase the texture, TSP was treated with 0.3% Flavourzyme or 0.1% Protamex for 10 or 20 min, respectively. The degree of hydrolysis for TSP treated with Protamex was higher than that treated with Flavourzyme. Hydrolysis was observed to increase as the reaction time was increased for both Flavourzyme and Protamex. The water holding capacity of TSP treated with Protamex for 10 min was the highest, and that treated with Flavourzyme for 20 min was similar to that of Protamex treatment for 20 min. The oil binding capacity of TSP treated with Protamex for 20 min was the highest. The hardness of the soy cutlets using TSP treated with Flavourzyme for 10 min was higher than that treated for 20 min, while that of Protamex treated for 20 min was higher than that treated for 10 min. The cohesiveness of the soy cutlets using TSP treated with Flavourzyme or Protamex for 10 min was higher than those treated for 20 min. The chewiness of the soy cutlets treated with Flavourzyme for 10 min was higher than for those treated for 20 min, while those treated with Protamex for 20 min was higher than those treated for 10 min. The springiness of TSP treated with Flavourzyme for 20 min was higher than those treated for 10 min, and higher than those treated with Protamex for 10 or 20 min. For sensory evaluation, the beany flavor of the soy cutlets treated with Protamex for 20 min was the weakest. The flavor and chewiness of both a pork cutlet and a soy cutlet treated with Protamex for 20 min were the best. In the overall quality, soy cutlets treated with Protamex for 20 min was the most desirable. In conclusion, soy cutlets treated with 0.1% Protamex for 20 min could be a reasonable substitute of pork cutlets.

Serial ultrasonographic examinations were daily performed from 15 days after ovulation until parturition to determine the time of first detection and ultrasonographic appearance of the fetal and extra-fetal structures in pregnant 10 Maltese, 10 Yorkshire Terrier, 15 Shih-tzu, and 10 Miniature Schnauzer bitches, respectively. Gestational age was timed from the day of ovulation (day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0ng/ml. The gestational length was (range: ) days and the geatational length was no statistically significant difference among bitches (p>0.05). The initial detection of the extra-fetal structures were; gestational sac at days , zonary placenta at days , yolk sac membrane at days , yolk sac tubular shape at days , and amniotic membrane at days , respectively. The time of the first detection of the extra-fetal structures were no statistically significant difference among bitches (p>0.05). The initial detection of the fetal structures were; embryo initial detection at days , heartbeat at days , embryo bipolar shape , fetal movement at days , limb buds at days , stomach at days , urinary bladder at days , skeleton at days , and kidney at days , respectively. The the time of the first detection of the fetal structures were no statistically significant difference among bitches (p>0.05). These results indicate the evaluation of the time of first detection and ultrasonographic characteristics of the gestational structures might be useful for pregnancy diagnosis, estimating fetal age, embryonic resorption, fetal monster, abnormal fetal growth and fetal viability, respectively.