국립원예특작과학원에서는 밝은 화색과 안정적인 화형의 생 육이 우수한 빨간색 스탠다드 장미 품종을 육성하기 위해 진한 적색 스탠다드 장미 품종 ‘엔드리스러브(Endless Love)’를 모 본으로, 꽃잎수가 많고 안정적으로 가시가 적은 밝은 노란색 ‘페니레인(Penny Lane)’ 품종을 부본으로 인공교배하였다. 37 개의 교배실생을 양성해 1, 2, 3차에 걸친 특성검정 및 현장실증 을 통해 꽃이 크고 화형이 안정적이며, 재배안정성 및 생산성, 절화특성이 우수한 ‘원교 D1-390’을 최종 선발하였다. 2023년 ‘루비레드(Ruby Red)’로 명명하여 국립종자원에 품종보호출원·등록되었다. ‘루비레드’ 품종은 밝은 적색(R53C)을 가졌으 며, 꽃잎수가 32.8매, 화폭과 화고는 각각 10.9, 5.9cm로 대조 품종보다 크다. 절화장은 평균 71.7cm, 절화수명은 약 16.7일, 수량은 연간 168대/m2로 대조품종인 ‘레드스퀘어(Red Square)’ 대비 절화장이 길고 절화수명도 2배 이상 길며, 수확량도 1.4배 우수하다. 2023년 국내 육성 장미 품종 서울식물원 관람객 대상 공동평가회에서 스탠다드 장미 중 우수한 평가를 받았으며, 현 장 실증 결과 농가별로 균일하고 우수한 수량과 절화품질을 보 였다. 절화용 장미 ‘루비레드’ 품종은 밝은 적색과 우수한 화형 을 가지는 품종으로 해외 대체 품종으로 국내에서 많이 재배될 것으로 기대된다.

Climate change has led to increased insect pests and pest distribution changes. Traditionally, chemical control using synthetic pesticides has been the main method for pest management, but the emergence of pesticide-resistant pests has become a problem. There is a need to develop new pest control agents to overcome these issues. Entomopathogenic fungi used in pest management have minimal environmental side effects and possess a mechanism of action distinct from that of synthetic pesticides. However, there is a need for the development of technologies to maximize the insecticidal effects of fungi against pests, and expressing and releasing dsRNA within the fungi can preemptively knock out the activation of the insect’s defense system, thereby enhancing the insecticidal effect. Controlling insect defense genes and using entomopathogenic fungi as bio-carriers forms a new pest management strategy. This approach, described as a “microbial insecticide agents development strategy of cassette concept, ” can versatilely modify genes and microbes. It is expected to overcome the limitations of synthetic pesticides.

In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa), clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus), red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences, the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostoc mesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species including E. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activity toward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failed to suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significant in some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contribute to further LAB research and improved vegetable sprout production.

고령운전자의 인지 및 신체 기능 저하로 인한 교통사고 증가는 점점 심각한 사회적 문제가 되고 있으며, 이 로 인해 면허증을 자진하여 반납하는 제도가 운영되고 있으나 보다 객관적인 평가 방법이 필요하다. 본 연 구에서는 다양한 주행 상황에서 발생하는 정적 및 동적 시각 자극에 대하여 운전자의 시각적 행동을 평가 할 수 있는 운전 시뮬레이션 주행 환경과 시나리오를 구현하는 것이 목표이다. 이를 위해 고령운전자의 주 행 특성 분석에 활용된 운전 시뮬레이션 시나리오를 기존의 연구 문헌들로부터 수집하였고, 정적 및 동적으 로 구분된 표적 자극에 따른 텍스트 네트워크 분석을 통해 주행환경과 시나리오를 재분류하였다. 또한, 유 사한 유형의 시나리오들은 보다 발생 빈도가 높고 표적 자극이 다양한 주행환경으로 병합하였다. 연구 결과 로 신호교차로, 비신호 교차로, 왕복 2차로, 왕복 4차로 주행환경에서의 전체 12종의 시나리오로 구성된 운 전 시뮬레이션 콘텐츠가 구현되었다. 신호등, 정차된 차량, 표지판 등으로 구성된 정적 시각 자극과 주행 중 인 차량, 무단 횡단하는 보행자 등의 동적인 시각 자극이 제시되며, 이에 대한 시선 탐색(Visual Detection), 시지각(Visual Perception), 시각운동기능(Visuomotor Function)의 정량적 측정을 통해 운전자의 시각 행동 평가가 이루어진다. 본 연구에서 제안하는 시나리오 구성 방법은 운전 시뮬레이션 콘텐츠를 구현하기 위한 새로운 접근 방식으로써 시각 자극에 따른 운전 능력 검사 환경 구축에 관한 기초 자료로 활용될 수 있을 것으로 기대된다.

비모란 선인장 ‘Ahwang’ 품종이 2016년에 국립원예특작과 학원에서 육성되었다. 증식력이 우수한 밝은 황색 ‘Ahwang’ 품종 육성을 위해서, 황색 ‘Hwangun’품종을 모본으로, 황색 ‘0930001’ 계통을 부본으로 하여 2012년 6월 25일에 교배하였다. 어린 비모란 선인장을 2014년 이전에 삼각주에 2번 접목하여 계통을 양성하였으며 2014년부터 2016년까지 총 3회에 걸쳐 생육특성을 조사하였다. ‘Ahwang’ 품종의 모구는 편원형 모양에 황색 구색(Y9A)이다. 모구는 평균 8.6개의 능(rip)과 2.7mm의 짧은 직립형 회색 가시를 가지고 있고 혹(tubercle)이 돌출된 형태를 띠고 있다. 10개월 재배 후 ‘Ahwang’ 품종의 구직경은 44.5mm였으며 자구는 평균 26.9개가 생성되었다. ‘Ahwang’ 품종은 모구 능마다 황색의 자구가 3-4개가 착생되었다. 2016년 육성계통 평가회에서 ‘Ahwang’ 품종은 기호도 점수 3.9을 받았다. 이 품종은 2018년 5월 16일 국립종자원에 등록되었으며 식물신품종보호법에 의해 품종보호(등록번호 7193)를 받게 되었다.

The purpose of this study was to analyze the current status and contents of color-related education programs as lifelong education and to determine the necessity of color education and directions for future development. The findings of this study, first show that the general welfare center, which is striving to develop abilities and promote welfare, was opened mainly for professional education, rather than hobbyoriented education. Accordingly, pricing was set at a lower level than the three institutions and centers, but without a variety of programs or hobby-oriented courses. Second, as the purpose of the set-up is to be established, the general welfare center should be considered an area for hobbies along with expertise, and various courses should be opened. Although the department store’s cultural center focused on programs for cultural and leisure use and promotion, it is necessary to extend the program to attempt to approach it in a short-term process rather than a one-time class. The lifelong education center established as a subsidiary of the university’ was opened as an area for of painting such as oil painting and watercolor and drawing which have much in common school should learn and start basic theory and practice, so there was much difference. Third, if it is changed to a developed form of color-oriented education according to the purpose of each center and institution presented in the results of this research, a good response will be possible to further improve the quality of life and develop professionalism and creativity.

최근 봄철 기온이 높아짐에 따라 마늘 뿌리응애 피해가 늘어나고 있는 실정이다. 이러한 뿌리응애 피해를 최소하 하고자 발생양상과 22종의 약제에 대하여 살비효과를 조사하였다. 마늘 재배시기 중 뿌리응애의 발생은 충북 보은 등 5지역에서 2015년부터 2017년까지 3년간 3월부터 6월까지 뿌리응애 발생밀도를 조사하여 발생양상을 확인하였다. 뿌리응애는 2016년에 가장 많이 발생하였으며, 발생시기는 월동 후 3월 상순부터 발생하여 6월 중순경까지 지속적으로 밀도가 높아지는 양상을 나타내었다. 조사된 5지역 중 가장 높은 밀도로 조사된 보은지역은 6월 상순경 주당 13.3마리가 발생하였다. 뿌리응애에 대하여 우수한 살비효과를 나타낸 약제는 분무처리시 약제처리 10일 후에서 dimethoate, azocyclotin, abamectin, hexythiazox, cabosulfan, spirotetramat, benfuracarb가 90% 이상의 살비효과를 나타내었다

The fact that flip-flops, one of many different types of unstable shoes, are light and relatively easy to put on, accounts for their popularity among people. But because flip-flops rely heavily on the support of a single thong between your first and second toes, they impose a huge amount of pressure onto lower leg. Thus in the following experiment we tried to examine the different effects of flip-flops and running shoes in terms of their effect on muscle activity and fatigue of tibialis anterior and gastrocnemius during walking. In order to measure an electromyogram we used Free EMG system. 10 men and 10 women in running shoes ran on treadmills for 15 minutes at 4.8km/h, 2 days later the same experiment was carried out, but this time, in flip-flops. p value turned out to be greater than .05 and thus there was no considerable difference between the effects of flip-flops and running shoes on muscle activity and fatigue during walking. Therefore we conclude that despite the fact that flip-flops are considered unstable, their effects on muscle activity and fatigue of tibialis anterior and gastrocnemius are negligible.

The purpose of this study was to examine the degree of knowledge of care workers working at long term care hospitals and nursing homes on pressure ulcer. A total of 81 care workers including 34 at long term care hospitals and 47 at nursing homes were surveyed. 24 questions were used to evaluate their degree of knowledge on pressure ulcer. Their knowledge on pressure ulcer scored 12.84 out of the total score of 24 points(SD=3.40), which was equal to 53.50 (SD=24.23) out of 100 points. Their knowledge on the prevention of pressure ulcer was highest among the subareas of evaluation. Their knowledge on pressure ulcer statistically significantly differed according to education on pressure ulcer(P<.05). A pressure ulcer is a skin disorder that may be prevented and cured. At this point when long term care facilities are rapidly increasing, care workers highlevel knowledge on and good management of pressure ulcer is very important. Practically educating them on pressure ulcer including the provision of recent, updated relevant knowledge will be necessary.

본 연구는 환경스트레스 저항성이 증진된 페튜니아를 개발하기 위하여 NDPK2유전자 도입 형질전환 계통 NDPK2-7-1와 SOD2 유전자 도입 형질전환 계통 SOD2- 2-1-1-35간의 교잡에 의해 획득된 후대들의 비생물적 스트레스 저항성을 조사하기 위해 수행되었다. 비 생물적 스트레스 유발원인 메틸바이올로젠(methyl viologen, MV) 100 μM과 200 μM 처리에서 교잡후대들은 그들의 교배 모본 SOD2 유전자나 NDPK2 유전자가 단독으로 도 입된 형질전환 계통이나 비형질전환체 보다 메틸바이 올로젠에 의한 피해를 적게 받았다. 이는 SOD2 유전 자나 NDPK2 유전자가 단독으로 도입된 형질전환 계 통간 교잡에 의해 획득된 후대들이 그들의 교배모본 (SOD2 유전자나 NDPK2 유전자가 단독으로 도입된 형질전환 계통)이나 비형질전환체 보다 산화적 스트레 스에 대한 저항성이 증진되었음을 증명해 준다고 할 수 있다. 이들 교잡후대들은 초장 등 11종류의 양적형질의 특성이 비형질전환체에 비해 약간 길거나 짧긴 하였지 만 비형질전환체와 거의 유사하였으며, 꽃 색갈이나 모양 또한 그들의 교배모본 (SOD2 유전자나 NDPK2 유전 자가 단독으로 도입된 형질전환 계통)이나 비형질전환 체와 차이가 없었다.

Testes‐derived unipotent male germ‐line stem (GS) cells can acquire multipotency under appropriate culture conditions to become mGS cells which can contribute to all three germ‐layers. This study was designed to investigate the epigenetic characteristics of mGS cells derived from adult mouse testes (maGS cells). The GS cells were isolated from 4 6 week DBA mouse and were cultured in Dulbecco’s modified Eagle Medium supplemented with 15% (v/v) fetal bovine serum, 1,000 U/ml LIF, 4 ng/ml GDNF at 37℃ in an humidified atmosphere of 5% CO2 in air to derive the maGS cells. The multipotency of maGS cells were verified by morphological and gene expression analyses, teratoma formation upon transplantation into nude mouse and in vitro differentiation ability. Bisulfite genomic sequencing revealed that GS cells had androgenetic DNA methylation pattern at the Igf2‐H19, Gnas‐Nespas , and Dlk1‐Dio3 imprinted gene clusters which changed to hemi‐zygotic embryonic stem (ES)‐cell like pattern in the maGS cells. Western blot analysis, using modification‐ and residue‐specific antibodies, revealed that both maGS and ES cells had similar level of histone di‐methylation at 4th and 27th lysine residue of histone 3 (H3K4me2 and H3K27me2) which represent “bivalent domain” for regulating self‐renewal and differentiation of mouse ES cells. Both maGS and ES cells also shared similar hisone modification for H3K9me2, H3K79me2, H3K9ac and H3K18ac. However, maGS cells had higher level of H3K- 36me2 and H3S10p. These data suggest that maGS and ES cells share several epigenetic characteristics but they also have their own unique epigenetic marks that may be useful as a molecular marker for their identification.

To find an alternative for synthetic pesticides, methanol extract from plant samples were tested for their insecticidal activity against insect. The extract of Asiasarum sieboldii had strongly insecticidal activity against Plutella xylostella. Roots of A. sieboldii were extracted with methanol, and the concentrated extract was partitioned with n-hexane, ethylacetate, n-buthanol and H2O. The highest activity was shown in the hexane fraction. Activity-guided fractionation led to the isolation of two amides from hexane fration through the repeated silica gel column chromatographic separations. From the interpretation of spectropic data including NMR, MS, IR, the chemical structures of compounds were determined as dodeca-2E,4E,8Z,10Z-tetraenoic acid isobutylamide and dodeca-2E,4E,8Z, 10E-tetraenoic acid isobutylamide. These compounds showed insecticidal activity on P. xylostella by 96.7% at 100ppm. The liquid formulation controlled on cabbage effectively. The extract and compounds from A. sieboldii showed insecticidal activity against Nilaparvata lugens. As a naturally occurring pesticide, A. sieboldii could be useful as a new botanic insecticide.

The purpose of this study was to analyze and compare the effect of resistance exercise and balance exercise on proprioception and WOMAC index of patients with degenerative knee osteoarthritis. A total of 40 subjects participated in this study. The subjects were diagnosed with degenerative knee osteoarthritis and all were more than 60 years old. They were divided into three groups. Group Ⅰ(n=8) was trained with resistance exercise, Group Ⅱ(n=6) was trained with balance exercise and GroupⅢ(n=6) was trained with range of motion as a control. The results of this study were as follows. It was significantly indicated that the resistance exercise group and balance exercise group elicited error-reduction on proprioception goal-angle (p<.05). There was a statistically significant difference on proprioception between resistance exercise group and control(range of motion) group. There was a statistically significant reduction on WOMAC index between resistance exercise group and balance exercise group (p<.05) and on the WOMAC index between resistance exercise group and range of motion group(p<.05). In conclusion, resistance exercise and balance exercise are effective on degenerative knee osteoarthritis and resistance exercise is the most effective for improving proprioception and WOMAC index. More research on the intervention according to the degree of degenerative knee osteoarthritis is needed.