본 연구는 산란후기 사료에 뽕잎의 수준별 첨가 급여가 산란계의 생산성, 계란품질 및 혈액성상에 미치는 영향을 구명하고자 시행하였다. 74주령 하이라인 브라운 산란계 360수를 4개처리 5반복 반복당 18수씩 배치하여 12주간 사양실험을 실시하였다. 처리구는 ME 2750kcal/kg, CP 15%인 기초사료에 뽕 잎분말을 0, 0.2, 0.4 및 0.8% 수준으로 첨가 급여하였다. 산란율과 난중은 매일, 사료섭취량과 계란품 질은 4주 단위로 조사하였고, 혈액성상과 난황지방산은 사양실험종료 후 채취하여 측정하였다. 전체 사양실험기간에서 산란율과 1일 산란량은 뽕잎 첨가구에서 증가하는 경향을 보였지만 처리구간에 통계적 차이는 없었고, 난중, 사료섭취량 및 1일산란량도 유의적 차이는 없었다. 사료요구율은 실험 전기간 뽕 잎첨가구에서 개선되는 경향이 있었으며, 특히 82~85주령에서는 통계적 차이를 확인하였다(p<0.05). 난백높이, 호유닛, 난각강도 및 두께는 처리구간 차이가 없었다. 불포화지방산은 뽕잎의 첨가수준에 따 라 현저하게 증가하였지만(p<0.05), 포화지방산은 감소되었다(p<0.05). 혈청 HDL 콜레스테롤은 0.4% 첨가수준에서 현저하게 증가하였고(p<0.05), 중성지방은 뽕잎 급여수준에 따라 매우 감소하는 경향을 보였다. 그러므로 본 연구결과는 뽕잎의 첨가급여가 산란후기 산란계의 생산성, 계란품질 및 강건성을 개선하는데 효과적인 것을 시사한다.

This study was conducted to evaluate the the efficacy of mulberry cake mixed diet on larval growth of Protaetia brevitarsis. As a result of adding 3, 5, and 10% mulberry cake to the fermented mulberry sawdust, the development period of the Protaetia brevitarsis larvae was 44.9days, 44.6days, and 41.8days, and the highest weight of larvae was 2.76g, 2.80g, and 2.89g, respectively. As a result of adding 3, 5, and 10% mulberry cake to the fermented oak sawdust, the development period of the Protaetia brevitarsis larvae was 46.7days, 41.6days, and 41.7days, and the highest weight of larvae was 2.65g, 2.65g, and 2.56g, respectively.

The mulberry for production of mulberry fruit, found the damage and quantity according to the level of damage by the A. mori, and through regression analysis, we set up a economic injury level. The level of damage by A. mori was to be artificially adjusted, for 2 percent, 5%, 8 percent, and 11 percent of leaf damage. Investigate, From May 31 to June 14, they compared the damage to each damage level and the growth and development and quantity. The survey found that the more damage level, the more severe the damage. The ratio of damage fruit was 5.1 percent, 11 percent, 13.2 percent, 23.9 percent, respectively. And the quantity was reduced by about 33% from 11 percent to 2,387 g/tree compared to the 2 percent to 3,557 g/tree. There's no difference in the damage caused by the sclerotium disease, and the growth and development, such as the length of the tree, the length of the branches, and the thickness of branches. The relationship between the damage level and the ratio of damage fruit was able to obtain relations with the linear regression, Y = 2.129552 X +0.569663(R2 = 0.98). It's the result of an analysis, which analyzed the damage level's relationship with the ratio of damage fruit have a high correlation rate. Therefore, It’s about the ratio of the quantity losses is 5 percent, the ratio of damage leaf is 2.08 percent. If the ratio of damage leaf is more than 2.08 percent, the damage will be required the control. It is judged.

본 연구에서는 뽕잎(Morus alba)을 노루궁뎅이 버섯균사체(Hericium erinaceum)로 발효시킨 열수 추출물(MA-HE)의 항산화 활성을 확인하고자 하였다. MA-HE의 항산화 활성을 DPPH radical, ABTS radical 소거활성 측정을 통해 알아보았다. 그 결과 DPPH radical 소거활성은 500 μg/mL 농도에서 61.73%, ABTS radical 소거활성은 250 μg/mL 농도에서 97.39%로 나타났다. MA-HE은 DNA의 산화적 손상을 효과적으로 억제하였다. 또한 생체고분자물질인 사람의 혈청단백질의 산화적 손상을 억제하였다. 세포에 H2O2를 처리하였을 때 세포생존율에 비하여 발효물을 100 μg/mL 농도로 전 처리한 세포생존율은 8% 증가했으며, 발효물을 50 μg/mL 농도로 처리했을 경우 세포 내 활성산소의 축적이 유의적으로 감소되었다. 본 연구 결과는 MA-HE이 항산화 활성을 가지고 있으며 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용도 뛰어난 것으로 사료되었다.

This study examined the quality characteristics of cookies prepared with mulberry leaf powder (0, 1, 3, 5, and 7%) substituted for flour. The pH of the cookie dough decreased significantly in response to the addition of all levels of mulberry leaf powder. The spread factor of the cookies also increased significantly with increasing mulberry leaf powder content added to the cookies recipe. In addition, the Hunter’s color L and a values decreased significantly with increasing mulberry leaf powder content, whereas the b value increased. According to hardness measurements, the substitution of 1~7% mulberry leaf powder resulted in increased hardness compared to the control. Moreover, the DPPH free radical scavenging activity of the cookies was increased significantly (p<0.05) with increasing levels of mulberry leaf powder. The sensory evaluation revealed cookies containing 3% to have the highest scores.

2015년부터 2016년까지 전북지역 오디 생산용 뽕나무에서 점박이응애(Tetranychus urticae Koch)와 명주달팽이(Acusta despecta Sowerby) 2종을 포함하여 총 33종의 해충이 조사되었다. 그 가운데 뽕나무총채벌레(Pseudodendrothrips mori Niwa)를 포함한 8종이 주요 해충 으로 분류되었다. 수확 전에는 총 25종이 조사되었는데, 그 가운데 뽕나무총채벌레(P. mori), 뽕나무이(Anomoneura mori Schwarz), 뽕나무깍지 벌레(Pseudaulacaspis pentagona Targioni Tozzetti), 뽕나무애바구미(Baris deplanata Roelofs), 뽕나무가지나방(Menophra atrilineata Butler), 점박이응애(Tetranychus urticae Koch) 6종이 주요 해충으로 분류되었다. 수확 후에는 총 25종이 조사되었는데, 그 가운데 뽕나무총채벌레(P. mori), 뽕나무깍지벌레(P. pentagona), 뽕나무명나방(Glyphodes pyloalis Walker), 미국흰불나방(Hyphantria cunea Drury), 점박이응애(T. urticae) 5종이 주요 해충으로 분류되었다. 오디용 뽕나무에서 발생하는 해충은 조사지역에 따라 달랐으나, 뽕나무총채벌레 등 주요 해충 8종과 베짱이(Hexcacentrus unicolor Serville), 뽕나무노린재(Malcus Japonicus Ishihara et Hasegawa), 썩덩나무노린재(Halyomorpha halys stal), 오 리나무좀(Xylosandrus germanus Blandford), 나방류 4종(Bombyx mandarina Moore, Euproctis subflava Bremer, Acronicta major Bremer, Agrotis trifurca Butler) 및 명주달팽이(Acusta despecta Sowerby)는 모든 조사지역에서 발생되었다.

This study was conducted to investigate the microbial loads in mulberry fruits depending on cultivation environment and fruit ripeness. The population levels of total aerobic bacteria in mulberry fruits collected from open field orchards were higher than those from three plots protected within plastic green houses. In regards to fruit ripeness, the levels of total aerobic bacteria in ripe black fruits were higher than those in unripe green and red mulberry. From the farms into where livestock animals were allowed to enter, Escherichia coli was detected in soil at a level of 4.26~4.94 log CFU/g and in mulberry fruits at 5.03~6.07 log CFU/g, while no coliform and E. coli were detected from where the intrusion of livestock was prevented. We also examined the density change of inoculated E. coli in mulberry fruits as they were becoming mature. While E. coli did not increase in green fruits, two and four log CFU/g increases at 20oC and 37oC, respectively, were observed with red and fully mature black mulberries during 48 hours incubation. To ensure the food safety of mulberry, it is suggested that the introduction of E. coli into a farm through livestock should be prevented and more hygienic caution should be taken especially when the fruits are ripe.

In this study, we aimed to develop a mixed tea prepared with roasted mulberry leaf and fruit using response surface methodology (RSM). Roasting of mulberry leaf was by 6 stages, as shown in Fig. 1; and mulberry fruit was roasted in 4 stages, as shown in Fig. 2. Subsequently, physicochemical measurements such as total polyphenol content, nitric oxide production content, and α-glucosidase inhibitory effect were obtained for each sample. Central composite design was applied to prepare samples containing varying contents of roasted mulberry leaf (RoML) and roasted mulberry fruit (RoMF); subsequently, sensory evaluation was conducted. The total polyphenol content of roasted samples (RoML and RoMF) were significantly higher than that of raw samples (RaML and RaMF), respectively. The nitric oxide (NO) production of roasted samples were significantly lower than that of control (LPS induced RAW 264.7 cell). The α-glucosidase inhibitory effect of roasted samples was significantly higher than that of raw samples, respectively. Based on the RSM estimation for determination of optimum ratio by sensory evaluation (taste, color, and flavor) among 13 mixed samples, the optimum mixing ratio of RoML and RoMF for taste, color, and flavor were 1.64 g (RoML) and 0.88 g (RoMF), 1.35 g (RoML) and 0.92 g (RoMF), 1.65 g (RoML) 1.03 g (RoMF), respectively. Based on results of three sensory evaluations, mixing ratio comprising 1.54 g of RoML and 0.92 g of RoMF is desirable for delicious tea with functionality.

오디를 포함한 베리류의 건강 기능성이 알려지면서 생산량 증가 및 가공식품 개발이 활발해지고 있지만 베리류과실의 특성상 상처 입거나 무르기 쉬워 생과로 유통되기 보다는 다양한 형태의 가공품으로 유통되고 있다. 베리류를 원료로 식물발효액을 제조하기도 하는데 대부분 소규모 이루어져 원료의 안전성 및 제조과정에 대한 위생관리가 시급하다. 본 연구는 오디 발효액 제조에 사용되는 원물이 유해 세균에 오염되었을 경우와 발효액으로 소량 포장되는 단계에서 유해 세균에 오염되었을 경우에 오디 발효액 발효과정 중 그리고 오염 발효액 보관과정 중 오염세균의 세균수 변화를 조사하기 위해 수행되었다. 오디 발효액이 발효되는 동안 원물에 접종된 E. coli는 시간이 지나면서 감소하는 양상이었다. 특히 오디 원물의 무게 대비 100%로 설탕을 혼합한 처리구 보다는 80%로 혼합한 처리구에서 E. coli의 세균수 감소가 급격하였는데, 설탕을 원물 무게의 80%로 혼합하였을 때 발효과정 중 생기는 총산도가 100% 혼합처리구보다 높음으로써 결과적으로 발효액의 pH가 낮아지게 되어 발생한 결과인 것으로 판단된다. 발효가 완료되어 발효액으로 제품화되는 과정에서 식중독세균에 오염되었을 경우에는 접종 균주별로 조금씩 다른 세균수 감소 양상을 보였지만 대체적으로 보관시간이 지남에 따라 감소하는 양상이었고, 특히 30℃에서 보관하였을 때에는 실험균주 모두 4일 이내에 사멸하였다. 따라서 오디 발효액 제품화시 소량 포장 후 30℃의 온도에서 일주일 정도 보관하는 것이 오디 발효액의 미생물학적 안전성을 높이는데 도움이 될 것으로 판단된다.

This study was conducted to investigate the quality characteristics and antioxidant properties of cookies supplemented with 1-4% (w/w) mulberry leaf powder (MLP). The pH and density of cookie dough were not significantly influenced by the increasing levels of MLP (p>0.05), while the moisture content appeared to decrease significantly (p<0.05). The spread ratio of cookies tended to decrease with increasing levels of MLP (p<0.05). Lightness (L*), redness (a*), and yellowness (b*) decreased significantly with a higher amount of MLP (p<0.05). The use of MLP significantly increased the hardness of cookies while 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzthiazoline- 6-sulphonic acid (ABTS) radical scavenging activities were significantly elevated (p<0.05). The consumer acceptance test indicated that the addition of 1% MLP had a favorable effect on the consumer preferences in all the attributes. Based on the overall observations, cookies with 1% MLP can take advantage of the functional properties of MLP without sacrificing consumer acceptability.

Recently, the incidence of cardiovascular diseases including high blood pressure and heart disease has increased with increased meat consumption in Korea. This study was performed to determine the optimal mixing ratio among ratios of 0, 1:0, 0:1, 1:1, 2:1, and 1:2 of mulberry leaf powder to mulberry fruit powder based on the anti-inflammatory effects. Then, the quality characteristics of the sauce for meat prepared with different mixing ratios (0, 1:1, 1:2, 1:3, 1:4, and 1:5) of mulberry leaf powder to mulberry fruit powder were assessed. The inhibitory effects of ML2MF1 (2:1 ratio of mulberry leaf powder to mulberry fruit powder) on NO and TNF-α production were superior to those of other mixing ratios. With respect to color values of the sauce for meat prepared with different mixing ratio of mulberry leaf powder to mulberry fruit powder, as the mulberry fruit powder mixing ratios was increased, redness was increased but lightness and yellowness were decreased. The sensory evaluation of ML1MF5S (1:5 ratio of mulberry leaf powder to mulberry fruit powder) sauce for meat showed the highest score with respect to color, smell, taste, harmony with meat and the overall preference. In conclusion, as the mulberry leaf powder mixing ratio was increased, the anti-inflammatory activities were increased. But sensory evaluation scores were increased as the mulberry fruit powder mixing ratio was increased. Therefore, by performing further study, the method to increase mulberry leaf powder in the mixing ratio for improving the sensory evaluation should be provided.

In this study, we determined the effect of the mulberry leaf powder on blood lipid metabolism and liver function improvement of mice fed a high fat diet. The mulberry leaf showed a moisture content of 10.74±0.56%, ash 9.67±0.56%, crude protein 25.24±1.02%, and crude fat 2.66±0.08%. In the group with high fat diet supplemented with mulberry leaf powder, serum low-density lipoprotein (LDL-cholesterol) and total cholesterol levels were significantly lower (p<0.05); also, serum phospholipid and aspartate amino transferase (AST) levels were significantly lower (p<0.05). Serum insulin and leptin levels were high in mice fed a high fat diet; however, addition of mulberry leaf powder 10% in the diet had no significant effect on blood insulin and leptin control. The results of the study suggested that various plant sterols in mulberry leaf improve lipid metabolism and liver function in ICR-mice.

Mulberry (oddi) is one of the most popular functional foods with many physiological components. This study investigated and compared the nutritional compositions and physiological activities of four mulberry fruits from Morus albo L. including Cheongilppong, Iksuppong, Suwonppong and Cheongsuppong (a new cultivar from Chungcheongbuk-do). To analyze the nutrient contents, mulberry fruits were freeze-dried. The results showed that the proximate compositions of the four mulberry cultivars ranged from 9.61~14.11% for moisture, 8.28~11.90% for crude protein, 3.70~4.86% for crude ash, 4.28~5.54% for crude lipid and 7.46~10.78% for crude fiber. The above proximate contents of mulberry cultivars were not significantly different. However, Cheongsuppong had the highest content of reducing sugar, 74.7%. Chungsuppong and Iksuppong showed higher contents of total polyphenol and anthocyanin than other mulberry cultivars, while all mulberry cultivars showed high antioxidant activities. Hypoglycemic effect had a slightly higher level in Suwonppong and Cheongsuppong than in the other samples. Taken together, the new cultivar “Cheongsu” oddi can be suggested as a potential source of functional food.

This study investigated the antimicrobial activity of methanol extract of mulberry leaf against 16 strains of mutans streptococci and four species of periodontopathogens: Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extracts or silica gel chromatography fractions of methanol-extracted mulberry leaf were evaluated by determining minimal inhibitory concentrations using an established microdilution method. The cytotoxicity of the extracts of mulberry leaf on KB cells was tested by the methyl thiazolyl tetrazolium assay. Chromatography fraction 12 displayed the most potent antimicrobial activity against all 16 strains of mutans streptococci, P. gingivalis, and P. intermedia. No KB cell cytotoxicity was evident up to 128μg/ml of fraction 12. The methanol extract had no antimicrobial activity against F. nucleatum and A. actinomycetemcomitans. These results suggest chromatography fraction 12 methanol extract of mulberry leaf could be useful in the development of oral hygiene products, such as dentifrice and oral hygiene solution, for the prevention of dental caries.

This study aimed to investigate the changes in antioxidant activities, quality characteristics, and storage stability of mayonnaise prepared with perilla oil with the addition of mulberry powder ranging from 0 to 5%. The antioxidant activities of perilla oil mayonnaise (PM), as determined by DPPH and ABTS radical scavenging activity, as well as FRAP and total phenolics content were improved by the addition of mulberry powder compared with the control sample. Antioxidant activities were dependent on the amount of mulberry powder added. Lipid oxidation of PM decreased with a higher amount of mulberry powder, based on measurements of peroxide value. The emulsion stability of PM increased with decreases in the amount of mulberry powder level. The a-value in PM color increased with increasing amount of mulberry powder. Sensory evaluation showed that the highest score in overall acceptability of PM was achieved with 4% mulberry.

본 연구에서는 효과적인 오디 세척 기술을 확립하기 위해 버블 침치 세척 시 세척 시간과 천연세척보조제의 첨가가 오디의 미생물 저감화와 품질특성에 미치는 영향을 분석하였다. 세척시간에 따른 측정 결과에서는 일반세균의 경우 비세척 대조군은 4.9×104 CFU/g였으나, 1분 세척 시 3.1×104 CFU/g로 약 40% 가량 저감화되었다. 대장균군과 효모 및 곰팡이의 경우에도 유사한 경향을 보였다. pH, 당도, 흡광도의 경우, 1분 세척 시에는 비세척 대조군과 비교해 거의 차이를 보이지 않았다. 천연세척보조제로서 염수의 적용에 따른 측정 결과에서 일반세균의 경우 1% 농도에서 대조군에 비해 약 77% 가량 저감화되었고, 대장균군은 모든 조건에서 비검출되었다. 효모 및 곰팡이는 1%에서 1.6×103 CFU/g로 약 68% 가량 저감화되었다. 천연세척보조제로서 식초액의 적용에 따라 일반세균은 식초 농도의 증가에 따라 확연히 감소하는 경향을 보였고, 10% 이상에서 크게 감소하였다. 대장균군은 5% 이상에서 비검출되었고, 효모 및 곰팡이는 10% 이상의 농도에서 검출되지 않았다. 또한, 식초액 10%에서 대조군과 비교해 품질특성의 차이를 보이지 않았다. 1% 염수와 10% 식초액을 병용 처리한 경우, 대장균군과 효모 곰팡이는 검출되지 않았으며, 일반세균도 90% 이상 저감화되었다. 결과적으로 소금1%와 식초10%를 병용처리하는 것이 매우 효과적인 것으로 판단된다. 또한 제균 효과가 뛰어난 초음파 처리와 비교해서도 손색이 없는 세척 효과를 보이는 것을 알 수 있었다.