The purpose of this study was to investigate antimicrobial, antioxidant, DPPH radical scavenging and SOD-like activity effect of Jubak(AFC) extract using hot water and ethanol solvent. All extracts from Jubak(AFC) had antimicrobial activities in the Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Antioxidant activities of the Jubak(AFC) using soybean oil emulsion was confirmed to be higher in the extract using ethanol and hot water than control and the antioxidant was practically effective when concentration of the Jubak was 200 ppm. Scavenging effect of DPPH radical of Jubak extracts showed inhibition effect was above 80%. SOD-like activity were higher in hot water extracts than in ethanol extracts. If keeping up Jubak`s study, it could have potential as a cosmetic raw material. Additionally, Jubak could be expected as functional material in food by different extract method.

The present study was evaluated the antibacterial effect of the combination of Coptidis rhizoma,Lonicerae Flos, and Paeonia japonica (1:1:1) extracts (CLP1000). Also, the effectiveness of CLP1000, dioctahedral smectite (DHS), and the combination of CLP1000 and DHS (CLPS1000) against E. coli O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 10% and 20% CLP1000 were inhibited the growth of E. coli O157:H7 by 30% and 47%, respectively. For 7 days after single challenge with E. coli O157:H7, forty female ICR mice were divided into four experimental groups which were orally administered with saline, 10% CLP1000, 10% DHS, and 10% CLPS1000, respectively. On the 3rd day, the number of E. coli O157:H7 in mouse feces was significantly decreased by administration of CLP1000 (p < 0.05), DHS (p < 0.05) and CLPS1000 (p < 0.001). On the 7th day, CLP1000 (p < 0.05) and CLPS1000 (p < 0.001) administration significantly decreased the number of E. coli O157:H7. According to the results of the present study, administration of CLPS1000 to mice can reduce the severity of E. coli O157:H7 infection. Also, it is suggested that CLPS100 represents a good candidate for the treatment of enteric infections in domestic animals.

The objective of this study was to evaluate the antifungal properties of xanthorrhizol (extract from curcuma xanthorrhiza) against Candida albicans. Some of the commercial products were evaluated for their ability to inhibit growth of C. albicans. OTC product containing povidone iodide and cosmetic grade product with surfactants were tested. Antifungal activity was shown in 1.56% of OTC product, however, there was no antifungal effect in cosmetic product. For the comparison, we tested several materials. Povidone iodide, lemon tea tree oil and xanthorrhizol showed antifungal activities against C albicans in 0.25, 0.062 and 0.007%, respectively. We also tested the antifungal effects of povidone iodide and xanthorrhizol in surfactant base. Test results revealed that 2.5% of povidone iodide and 0.156% of xanthorrhizol showed similar antifungal effects. These findings support the application of xanthorrhizol for vaginal cleanser or personal cares using antifungal effect.

The objection of this research is to conform of practicable possibility and recycling of producing junk after citrus fruits is processed. In the middle of practicable possibility, with verification of antibiosis that tactiling sensibility in a microorganism. With extracting d-limonene oil that have 70~90% a component of oil out of junk citrus peel, making certain the about 12000ppm(1.2%) concentration of it. By means of antibiosis property over the a pathogenic bacterium as well as a residence bacterium, considerating the limit of application against daily living supplies needs to antibiosis. Antibiosis effect of a stationing bacterium in the body permanently and by means of antibiosis verification of special bacteria Propionibacterium Acnes that causative skin trouble is selected, in based the antibiotic sencitivity test check up result of minimal inhibitory concentration(MIC).

C. obtusa, an essential oil produced in Jeollanamdo Gangjin, is the most effective plant oil for easing Sick House Syndrome, having an effect similar to a therapeutic walk in the woods and acting as a sterilizer. In light of its medicinal importance, its composition, antibacterial activity and ability to mitigate an offensive odor have been analyzed. Through GC-MS, I confirmed that it contains approximately 50 components, 80.30% of which are various monoterpenes. It was effective at alleviating malodors from formaldehyde, ammonia, trimetylamine and methylmercaptan as determined in colorimetric gas detection tube tests in comparison with lemongrass and clove leaf essential oil. I obtained from Minimal Inhibition Concentration(MIC) of C. obtusa, higher concentrations will lead to increased antibacterial activity. Therefore it is estimated that utility value of it will be grow more and more.

목적: 본 연구는 안경수건에 함유된 은나노 입자의 항균효과를 연구하기 위해 수행되었다. 방법: 은나노 입자가 함유된 안경수건과 함유되지 않은 안경수건의 세균수를 비교하였다. 결과: 은나노입자가 함유된 안경수건에서 뚜렷한 세균수의 감소가 관찰되었으며, 이 결과는 안경렌즈에 존재하는 세균에 대해 은나노 입자가 높은 항균성을 가짐을 의미한다. 결론: 가까운 미래에 나노기술을 이용한 항균작용 안경수건의 개발이 기대된다.

Anti-microbiological effects of xanthorrhizol, ie., extract from curcuma xanthorrhiza and extract from houttuynia cordata Thunb. against influenza virus and E. coli O157 were tested. From the influenza experiments, the effects were shown above 93 % in case of houttuynia cordata Thunb. extract, however, the effects was not shown in case of xanthorrhizol. The effects were sustained in mixtures of houttuynia cordata Thunb. and curcuma xanthorrhiza extracts. We also tested the anti-microbiological effects of hand sanitizer containing houttuynia cordata Thunb. and xanthorrhizol. The effect of hand sanitizer containing 2,000 ppm of xanthorrhizol and 500 ppm of houttuynia cordata Thunb. extract was better than that of commercialized foreign product.

The purpose of this study was to investigate the antibacterial effect of films on shelf-life and microbiological Safety of pork meat. Effects of antimicrobial films against total aerobic bacteria, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus in pork meat were evaluated during storage of 14 days at 5℃, 10℃ and 15℃. Antimicrobial films were developed with addition of a natural substance, wasabi extracts (Wasabia japonica). At 5℃ storage, growth of total aerobic bacteria, E. coli O157:H7, L. monocytogenes were inhibited higher than at 10 and 15℃. Especially, the numbers of S. Typhimurium and S. aureus were increased gradually at 5℃ even in the control sample, and it takes more than 14 days to increase in every sample upto 6 log10 cfu/g. The higher antimicrobial effects of the films were observed at storage of 5℃ than at 10oC and 15℃. There was a limit of a single treatment of antimicrobial film to prolong shelf-life of pork meat. The synergistic effect of antimicrobial film were observed with addition of refrigeration at 5℃.

본 연구 결과 동결건조된 감귤과피 70% 메탄올 추출물의 총 폴리페놀 함량은 836.8 ㎎%였으며 감귤과피 추출물 0.01, 0.02와 0.1%의 EDA는 각각 89.6, 86, 81.3%로 높았으며 아질산염 소거능은 pH 1.2에서 34.4%, pH 7.0에서 19.5%를 나타내어 산성 영역에서 높은 소거작용을 보였다. 또한 이들 추출물의 항산화효과는 옥배유의 경우 TBHQ 〉 BHT 〉 TOC 〉 ChEx 〉 EaEx 〉 EtEx 〉 WaEx 〉 Control 순이었고, 채종유의 경우 TBHQ 〉 ChEx 〉 EaEx 〉 BHT 〉 EtEx 〉 WaEx 〉 TOC 〉 Control 순이었다. 두 유지모두에서 에틸아세이트추출물(EaEx)과 클로로포름추출물(ChEx)이 에탄올추출물(EtEx)과 물추출물(WaEx) 보다 항산화 효과가 높았으며 첨가농도가 증가함에 따라 항산화 효과도 증가하는 경향을 보였다. 그리고 감귤과피의 각 용매별 추출물의 공액이중산가도 에틸아세테이트추출물(EaEx)과 클로로포름추출물(ChEx)이 높았으며 이들의 항산화 효과는 TBHQ보다는 낮았으나 BHT, tocopherol과 비교하였을 때 거의 유사하거나 좀 더 높은 경향을 보였다. 한편 감귤과피 추출물은 우수한 항균력을 보여주었고 농도가 상승함에 따라 항균효과도 상승하였으며 이 효과는 그램 양성, 음성균주에서 고르게 나타나 천연 항균소재로서의 이용가능성을 확인하였다. 이상의 결과에서 감귤 과피는 총 폴리페놀 함량과 전자공여능 활성이 높아 항산화효과와 항균효과가 우수하게 나타나 감귤 과피의 기능성 이용도가 매우 높을 것으로 생각되며 감귤 가공 후 생기는 부산물들의 식품산업에서의 유용성도 높을 것으로 사료된다.

Most of cosmetics are emulsion products that contain the source of nutrition vegetable oil, mineral oil, natural extract and carbohydrate etc. There are many possibilities to be contaminated by microbials. We investigated the effect of antimicrobial and minimum inhibitory concentration(MIC) with thiamine dilauryl sulfate(TDS), which was prepared to use cosmetic lotion formulation. Staphylococcus aureus(S. aureus) and Escherichia col(E. coli) were used as test organism. MIC value of TDS was determined aganist microorganism for the growth inhibition by concentration of TDS. From the MIC results, antimicrobial effect of TDS was generally more effective to gram positive than gram negative. Antimicrobial effect with pH value against some microorganism appeared in the following order : pH 5 > pH 6 > pH 7. It showed strong antimicrobial activities against S. aureus, and weak antimicrobial activities against E. coli. If it was possible to determine the formulations with TDS, it would be effective to reduce the artificial preservatives.

본 연구는 시판되는 증자 녹차의 에탄올 추출물과 에탄올 추출물로부터 분획한 분획물로부터 최근 병원성 식중독균으로서 문제시되고 있는 E. coli O157:H7균에 대한 항균성을 검색하였다. 에탄올 추출물로부터 그 유효성분을 조사하기 위하여 핵산, 에틸아세테이트, 클로로포름 및 물로서 분획한 후 MIC를 검색한 결과 에틸아세테이트 분획물이 500μg/disk 농도로 가장 강한 활성을 나타내었으며, 헥산 분획물은 활성이 없었다. 가장 강한 항균력을 나타낸 에틸아세테이트 분획물의 농도별 항균력 및 pH에 대한 영향을 조사한 결과 균종에 따라 차이는 있지만 3균주 모두 250-2000μg/disk 농도에서 새육이 억제되었다. 또한 E. coli O157:H7균은 산성조건에서는 생육이 가능하지만 pH 10 이상의 강한 알칼리 조건에서는 생육이 크게 억제되었다. 35℃에서 E. coli O157:H7 933과 932균에 대한 에틸아세테이트 분획물의 농도별 생육저해 효과를 검색한 결과 두 균주 모두 250μg/ml , 500μg/ml의 농도에서는 12시간 이후부터 생육하기 시작하였으나 1000μg/ml의 농도에서는 생육이 크게 저해되었다.

Extraction of Natural Artemisia by using super critical carbon dioxide is operated under 40-50℃ and 200-250 atm, thus, conversion rate is very low as 7wt% while high energy is being wasted. When Natural Artemisia Extract is applied in the control of microbe, concentration and reaction time greatly affected to the growth of microbe. Especially, when refined oil concentration from Natural Artemisia added more than 1,000ppm, staphylococcus aureus and fungi are terminated completely. Thus, it is proven that Natural Artemisia Extract has antimicrobial effect.

1. 무좀균에 대한 각 시료들이 항균력으로는 terbinafine > 100%-bamboo oil > 50%-bamboo oil 순으로 나타내었다. 무좀균에 대한 Bamboo oil에 대한 영향으로는 T. M > E.F > T.R의 순서대로 항균력을 나타내었으며, 특히 T.M에 대하여 가장 우수하게 나타내었다. 2. 비듬균에 대한 각 시료들이 항균력으로는 terbinafine > 100%-bamboo oil > 50%-bamboo oil 순으로 나타내었다. 비듬균에 대한 Bamboo oil에 대한 영향으로는 P.O-75 > P.O-77의 순서대로 항균력을 나타내었으며, 특히 P.O-75에 대하여 가장 우수 하게 나타내었다. 3. 50%-bamboo oil은 무좀균과 비듬균에 대하여 항균력이 있는 것으로 나타내었으며, 100%-Bamboo oil의 항균력과 비슷하게 나타내었으며, 경비 절감 측면에서 기대를 걸 수 있을 것으로 사료된다.