Understanding changes in fermentation characteristics and microbial populations of forage silage during ensiling is of interest for improving the nutrient value of the feed for ruminants. This study was conducted to investigate the changes in fermentation characteristics and bacterial communities of whole crop rice (WCR) silage during the ensiling period. The chemical compositions, pH, organic acids and bacterial communities were evaluated at 0, 3, 6, and 12 months after ensiling. The bacterial communities were classified at both the genus and species levels. The dry matter content of WCR silage decreased with the length of storage (p<0.05), but there was no significant difference in crude protein and NDF contents. Following fermentation, the pH level of WCR silage was lower than the initial level. The lactic acid content remained at high levels for 3 to 6 months after ensiling, followed by a sharp decline at 12 months (p<0.05). Before fermentation, the WCR was dominated by Weissella (30.8%) and Pantoea (20.2%). Growth of Lactiplantibacillus plantarum (31.4%) was observed at 3 months after ensiling. At 6 months, there was a decrease in Lactiplantibacillus plantarum (10.2%) and an increase in Levilactobacillus brevis (12.8%), resulting in increased bacteria diversity until that period. The WCR silage was dominated by Lentilactobacillus buchneri (71.2%) and Lacticaseibacillus casei (27.0%) with a sharp reduction in diversity at 12 months. Overall, the WCR silage maintained satisfactory fermentation quality over a 12-month ensiling period. Furthermore, the fermentation characteristics of silage were found to be correlated to bacterial microbiome.

Gastrodia elata has been used in traditional Chinese medicine for treating headaches, dizziness, and convulsive illness for centuries. G. elata has traditionally been processed by steaming or blanching to increase the content and quality of its main ingredients. This study aimed to identify changes in physicochemical properties and active ingredients of G. elata depending on the steaming time. Data of this study could be used to develop traditional medicine and health foods. No steaming was used as a control. Steaming time was 5, 10, 20, 30, 60, or 120 min. The drying yield according to the steaming time ranged from 20.2% to 22.9%, with the lowest drying yield at 120 min. As the steaming time increased, gastrodin content increased more than that in fresh G. elata due to inhibition of β-glucosidase enzyme activity, 4-hydroxybenzyl alcohol condensation, and parishin decomposition. Steamed G. elata did not show higher total polyphenols, total flavonoids, or ABTS radical scavenging activities than fresh G. elata even with an increase of steaming time. The steaming time to improve the quality of G. elata may varied depending on the size of G. elata. Thus, it is important to set the steaming time taking these characteristics into consideration.

The heat transfer characteristics of double-pipe spiral heat exchanger were investigated by various curvature sizes, experimentally. The three different sizes of heat exchanger were made and tested with water as a working fluid to analyze the heat transfer characteristics. The heat transfer rates, overall heat transfer coefficient and pressure drop were analyzed with various heat exchanger sizes (i.e., curvature ratios). As result, the heat transfer rate increased with increasing the size of the heat exchanger as the flow rate increased due to increasing the area size of heat transfer. However, the overall heat transfer coefficient and pressure drop increased with decreasing the heat exchanger size (i.e., increased curvature ratio) due to the enhanced centrifugal force and inertia.

In this study, we examined the effect of orifice diameter on atomization performance such as SMD(Sauter Mean Diameter), MMD(Mass median diameter), particle size distribution, spray distance, and spray angle when water was sprayed through a siphon nozzle. In addition, the behavior characteristics of spray were analyzed using the CFD(Computational Fluid Dynamics) commercial program. In the downstream direction of the flow, the dispersion and diffusion power of the droplets increased, greatly improving atomization performance. The spray spread in the radial direction when the jet velocity of water increased. As a result, atomization performance improved as the jet velocity increased.

To improve usability of super sweet corn, extracts were prepared with hydrolytic enzyme and changes in physicochemical and antioxidant properties were analyzed. Soluble solids and reducing sugars contents were higher in all enzyme treatment groups than in the control. When enzyme treatment time increased, contents of soluble solids and reducing sugars were also increased. There was no significant difference in lightness between treatment groups, with redness showing the highest value in the control and yellowness showing the highest value in the invertase treatment group. Free sugar content in the control was the lowest. However free sugar content in the enzyme combination treatment group was increased by more than four times compared to that in the control. Contents of ascorbic acid, flavonoids and polyphenols were higher in the enzyme treatment group than in the control. In particular, the enzyme combination treatment group showed the highest content. DPPH and ABTS radical scavenging abilities were significantly higher in all enzyme treatment groups than in the control. Radical scavenging abilities of cellulase treatment group and enzyme combination treatment group showed high activity. The activity increased when enzyme treatment time increased. The combined enzyme treatment method for super sweet corn was suitable for food processing.

The characteristics and spore production of Gonji7ho, Bunhong, and Sunjung fruiting bodies were assessed at different growth stages. The shape of the Pleurotus species fruiting body starts out short and small, then takes on a typical mushroom shape as it grows. Gonji7ho has a long stalk, Bunhong has a short stalk and a wide cap, and Sunjung's cap and stalk dimensions are intermediate. Each variety displayed deep color at the beginning of growth but became steadily lighter with continued growth. The shape of the linkage between the mushroom stalk and cap changed from an initial central position to a lateral position after the growing stage. Gonji7ho cap diameter increased 7-fold from 15.5 mm (5 days of growth) to 37.9 mm (9 days of growth). Growth rates for each growth day measured using the growth percentage of the previous day were 285.5% (5 → 6th day), 182.2% (6 → 7th day), 129.4% (7 → 8th day), and 103.8% (8 → 9th day). This trend was also observed in Bunhong and Sunjung, but Bunhong’s growth rate was more rapid (4.9 fold on day 6, 2.7 fold on day 7) and continued to increase through day 9. Harvest yield, which is of greatest interest to farmers, displayed a similar trend spanning the growth period, as did cap diameter. Gonji7ho harvest yield increased rapidly until day 7 of growth (more than 177%), then growth slowed down beginning around day 8, and further decreased on day 9 (98%). Similar trends were observed in Bunhong and Sunjung. Bunhong showed characteristic rapid growth in harvest yield (4.9 fold compared to the previous day on day 6 and 2.7 fold on day 7), and the increase continued through day 9. A decrease in mushroom harvest yield commonly seen in the late growth stage is thought to be due to the death of some mushrooms and decomposition of cap tissue. Basidiospore content increased with number of growth days but decreased after day 8. Gonji7ho yielded the highest production on day 7 of growth, coinciding with harvest time, with 209,000,000 spores. This trend was also observed in Bunhong and Sunjung. These results will provide researchers with basal data and guide farmers in selecting the optimal harvest day.

This experiment investigated the effects of feed additives of Sasa quelpaertensis Nakai (SQN) extract on Landrace pigs on economic traits such as the quality, physiological characteristics, and productivity. Sixteen pigs with an average age of 154 days were selected as experimental subjects. The experiment was conducted by dividing the group into eight pigs for the supplementation group, feeding with SQN extract, and another eight for the control group feeding without SQN extract. Water was fed ad libitum. On the 30th day, there was no significant difference between meat quality and productivity. However, the glucose and thyroxine were statistically lower with the supplementation group than with the control group (p<0.05). Also, the levels of creatinine difference between 1.18 ± 0.12 ㎎/㎗ with the supplementation group and 0.70 ± 0.06 ㎎/㎗ with the control group (p<0.05). However, all serum biochemistry values were within a normal range, with no health problems. The present study will help solve the problem of reducing the diversity of plant species in Halla Mountain by increasing the availability of the SQN as a pig feed additive.

This study was performed to investigate immune changes by comparing the proportion and function of immune cells in the blood under high-temperature period and convalescence temperature period in Holstein dairy cows. The experiment was conducted using Holstein dairy cows of five animals per group (60 ± 20 months old, 175 ± 78 non-day) from the National Institute of Animal Science at high-temperature period (THI: 76 ± 1.2) and convalescence temperature period (THI: 66 ± 1.3). Complete blood count results showed no change in the number of immune cells between groups. In the analysis using Flow Cytometry of PBMCs, no significant differences were observed among B cells, Helper T cells, cytotoxic T cells, and γδ T cells between groups. However, there was an increase in Th17 cells producing IL-17a, while Th1 cells decreased during the convalescence temperature period. The results of gene expression analysis using qRT-PCR in PBMCs revealed an increase in IL-10 during the convalescence temperature period, while a decrease in HSP70 and HSP90 was observed. In conclusion, the increased expression of IL-10 and the decrease in HSP expression suggest the possibility of a weak recovery from heat stress. However, the lack of observed changes in B cells, T cells, and other immune cells indicates incomplete recovery from heat stress during the convalescence temperature period.

한우에게 급여하는 사료 내 에너지원의 종류에 따라 반추위 미생물의 아미노산 조성에 차이가 있는지를 조사하고자 본 연구를 수행하였다. 국내 한우 비육우 사육에 주로 이용되는 에너지원 사료인 옥수수(T1), 생미강(T2), 소맥(T3) 그리고 소맥피(T4)로 반추위 환경과 유사한 연속식 배양기를 이용하여 72시간까지 배양을 진행하였다. 배양이 진행되는 동안 6시간 간격으로 배양액의 발효 성상을 확인하였으며 pH, NH3-N이 일정하게 유지되는 것을 확인하였으며, 배양 종료 시점인 72시간대에 미생물체 단백질 합성량(MPS), 미생물의 아미노산 조성 및 미생물 균총 변화를 분석하였다. 배양액의 pH는 모든 처리구에서 배양 기간동안 5.5~7.0을 유지하였다. Total VFA 농도는 T1이 23.13 mM으로 가장 낮았고, T4가 29.93 mM으로 가장 높았다(p<0.05). A (acetate) :P (propionate) 비율은 T1이 1.48로 가장 낮았으며, T4가 2.81로 가장 높게 나타났다 (p<0.05). Butyrate는 T1이 2.37 mM으로 가장 낮았으며, T2, T3 그리고 T4는 4.37~4.58 mM으로 차이가 없었다(p<0.05). MPS는 T2 (332.5 mg/L)와 T3 (320.2 mg/L)가 높았고 T1 (137.5 mg/L)와 T4 (154.2 mg/L)로 낮게 나타났다(p<0.05). DGGE band 분석결과 모든 처리구는 57.5 % 이하의 유사도가 나타났다. 미생물의 총 아미노산 함량은 T1 (31.59 %)과 T3 (31.33 %)가 가장 높았으며, T2 (20.09 %)가 가장 낮았다 (p<0.05). 이는 급여된 사료 내 총 아미노산 함량과 반대되는 결과로 나타났다. 따라서, 본 연구에서 한우 급여 에너지원 사료에 따른 반추위 내 미생물 발효 특성과 미생물체 단백질 합성량이 미생물 군집에 영향을 미치며 이에 따라 미생물의 총 아미노산 함량에 영향을 미친다는 것을 확인하였다.

In the last five decades, there has been a consistent decline in the total catch of fisheries in the Korean jurisdiction since the peak in 1986. The decline in catch slowed and slightly rebounded in the 2000s, but changed back to a decline in the 2010s. As indicators that can identify changes in the marine ecosystem, trophic level (TL), biodiversity index (H'), and the ratio between pelagic fish and demersal fish (P/D) were analyzed by each local marine ecosystem. There were some different changes in each local marine ecosystem, but the mean TL and H' decreased and P/D increased in general in Korean waters. Demersal fish, which were dominant in the 1970s and 1980s, declined, and small pelagic fish and cephalopods have dominantly changed since the 1990s. However, these changes are not simple, and they are fluctuating in complex ways relating to each marine ecosystem and the timing. It is believed that changes in marine ecosystems in Korean waters are likely caused by a combination of fisheries and climate change. The ecosystem indicators reflected a change in the total catch, a sharp drop in catch of demersal fish, and increasing catch of pelagic fish since the mid-1980s.

The change in the performance of the range hood according to the pressure change was investigated through a comparative experiment, and the conclusion is as follows. KS C 9304: In 2020, static pressure was applied at 100 Pa according to the fan standard, and the test was conducted by varying the outlet diameter and power consumption. In the case of Motor power 55W, the air volume value was 184.6 when the outlet diameter was 125mm. As a result of the test, if the power consumption is 52.0W, the diameter of the outlet is 125mm, and the power consumption is 60.8W, and the diameter of the outlet is 100mm, it meets 160 or more and less than 200 (constant pressure 100Pa).

The purpose of this study was to investigate the effects of sterilization, storage period and washing yuzu, according to the washing method, during the storage period. The results showed that the fungus size increased as the storage period increased, and no mold occurred in the yuzu washed with ozone water until 20 days. After 30 days of storage, a mold of 124.1±13.9 mm2 was observed. The no-treatment sample had a fungus of 814.5±72.8 mm2 in size on day 0 and the fungus the largest fungus was 6,362±636.7 mm2 on day 30. In the case of water treatment, the fungus was 286.4±31.5~4,836.4±484.6 mm2 in size. The results of the study confirmed that washing yuzu with ozone water has a sterilizing effect.

Although the Ti–6Al–4V alloy has been used in the aircraft industry owing to its excellent mechanical properties and low density, the low formability of the alloy hinders broadening its applications. Recently, laser-powder bed fusion (L-PBF) has become a novel process for overcoming the limitations of the alloy (i.e., low formability), owing to the high degree of design freedom for the geometry of products having outstanding performance used in hightech applications. In this study, to investigate the effect of bulk shape on the microstructure and mechanical properties of L-PBFed Ti-6Al-4V alloys, two types of samples are fabricated using L-PBF: thick and thin samples. The thick sample exhibits lower strength and higher ductility than the thin sample owing to the larger grain size and lower residual dislocation density of the thick sample because of the heat input during the L-PBF process.

Because magnets fabricated using Nd-Fe-B exhibit excellent magnetic properties, this novel material is used in various high-tech industries. However, because of the brittleness and low formability of Nd-Fe-B magnets, the design freedom of shapes for improving the performance is limited based on conventional tooling and postprocessing. Laserpowder bed fusion (L-PBF), the most famous additive manufacturing (AM) technique, has recently emerged as a novel process for producing geometrically complex shapes of Nd-Fe-B parts owing to its high precision and good spatial resolution. However, because of the repeated thermal shock applied to the materials during L-PBF, it is difficult to fabricate a dense Nd-Fe-B magnet. In this study, a high-density (>96%) Nd-Fe-B magnet is successfully fabricated by minimizing the thermal residual stress caused by substrate heating during L-PBF.

세계 5대 갯벌해역으로 유명한 한국 서해 경기만의 기후변화 영향을 파악하고, 기후변화 영향을 최소화하기 위한 적응방안 연 구하였다. 경기만의 기후변화 영향으로 2100년에 수온은 1.2 ℃ 증가하고, 염분이 1.1 PSU 감소하며, 해수면은 35.2 cm 상승하는 것으로 예 측되었다. 또한 해수면 상승의 효과로 150.5 km2의 해안지역이 침수될 것으로 예상되었다. 기후변화로 인한 경기만 생태환경 영향을 최소 화하기 위한 적응대책으로는 1) 경기만 자체 자연환경의 적응능력 유지를 위한 지원, 2) 생물 서식지 확보를 위한 인간 활동 조정 등 두가 지 방안이 제시되었다.

PURPOSES : For most local governments, including that of Gangwon-do, the establishment of an organized pavement management system is insufficient, resulting in problems such as inefficient distribution and use of maintenance budgets for deteriorated road pavements. In this study, we aimed to contribute to the establishment of a more reasonable road maintenance strategy by developing a model for predicting the annual international roughness index (IRI) change for national highway asphalt pavements in Gangwon-do based on big data analysis. METHODS : Data on independent and dependent variables used for model development were collected. The collected data were subjected to exploratory data analysis (EDA) and data preprocessing. Independent variable candidates were selected to reduce multicollinearity through correlation analysis and specific conditions. A final model was selected, and sensitivity analysis was performed. RESULTS : The final model that predicts annual IRI change uses independent variables such as annual temperature range, minimum temperature, freeze-thaw days, IRI, surface distress (SD), and freezing days. The sensitivity analysis confirmed that the annual IRI change was affected in the order of annual temperature range, minimum temperature, freeze-thaw days, IRI, SD, and freezing days. CONCLUSIONS : Road maintenance can be performed rationally by predicting future pavement conditions using the model developed in this study. The accuracy of the prediction model can be improved if additional data, such as material properties and pavement thickness, are obtained in future studies.