The purpose of this study is to optimize the composition of the medium for turmeric fermentation and to select competent turmeric fermentation strains using bacterial isolates from kimchi. Initially, 30 isolates from kimchi were cultured in 5% (w/v) yeast extract and 1% (w/v) maltodextrin to determine viability. As a result, eight strains showed a tendency to maintain viability until the fifth day of fermentation. Subsequently, the eight isolates were fermented in an optimum medium for turmeric fermentation, 5% (w/v) yeast extract, 1% (w/v) maltodextrin, and 5% (w/v) turmeric for seven days to determine the viable cell count and antioxidant capacity. The antioxidant capacities of turmeric fermented by the eight isolates were similar or higher than turmeric fermented by Lactococcus lactis KCTC 2013, while maintaining high viable cell counts of both the eight isolates and L. lactis KCTC 2013 until the seventh day of fermentation. The antioxidant capacities of the selected five strains during fermentation might increase possibly due to the biological conversion of active compounds in turmeric by fermentation. Consequently, a total of five strains of the isolates showing higher antioxidant capacity (4.81±0.19-5.81±0.04 VCE/mL) than fermentation day 0 were selected for fermentation of turmeric.

울금(Curcuma longa L.)의 생리활성 및 지질과산화 저해능에 미치는 영향을 확인하고 기능성 식품 소재로 활용 가치를 검토하기 위하여 연구를 수행하였다. 총 카로티노이드(total carotenoid) 함량은 1.581±0.005 mg β-carotene equivalents (BCE)/g dry weight으로 관찰되었다. 70% 메탄올, chloroform:methanol (CM, 2:1, v/v), 에틸 아세테이트(ethyl acetate, EA) 3가지 용매를 사용한 추출 수율은 70% 메탄올(16.54%), CM (5.64%), EA (4.14%) 순으로 나타났다. 총 페놀 함량은 EA, CM 및 70% 메탄올에서 각각 106.287, 90.614 및 18.527 mg gallic acid equivalents (GAE)/g의 함량으로 나타났으며, 추출 용매 별 항산화능은 0.2, 0.4, 0.6, 0.8 mg/mL의 농도로 측정하였고, 양성대조구로 사용된 BHA (butylated hydroxyanisole) 및 trolox 보다는 낮은 항산화능을 보였다. Nitric oxide (NO) 라디칼 소거능은 70% 메탄올에서 28.65~48.43%, CM 18.86~55.10%, EA에서 15.68~56.25%로 관찰 되었다. Nitrite (NO2) 소거능은 70% 메탄올, CM 및 EA 순으로 나타나 EA 추출물에서 유의적인 차이를 보이며 강한 NO2 소거능을 나타내었다(p<0.05). β-carotene 탈색 저해능은 70% 메탄올, CM 및 EA에서 각각 1.64~23.79%, 6.99~41.16% 및 10.20~48.52%로 관찰되었다. 한편, 지질과산화 저해능은 70% 메탄올, CM 및 EA 추출물에서 높게 측정되었다. 이상의 결과로 미루어 볼 때, 울금은 기능성 식품 소재로서 활용 가치가 있을 것으로 판단된다.

Streptozotocin (STZ, 45 mg/kg body weight)으로 유도된 Sprague Dawley (SD)계 수컷 흰쥐에 5% 울금을 섭취시켰을 때, 혈청 크레아티닌(creatinine) 및 blood urea nitrogen (BUN)의 농도와 alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aminotransferase (AST, ALT), 카탈라아제(catalase), 아밀라아제(amylase) 및 리파아제(lipase) 활성에 미치는 영향을 알아보기 위해 본 실험을 수행한 결과, 기본식이를 급여시킨 대조군(control)인 정상군은 BD군, 기본식이 실험군에 5% 울금 급여군(BT군), 당뇨 유발 실험군(BS군)인 질환 모델 대조군(control-diabetic)과 질환 실험군(diabetic, BS군) 에 5% 울금을 급여시킨 실험군(ST군)으로 나누었다. 흰쥐의 혈청 크레아티닌 및 BUN 농도와 ALP, AST 및 ALT 활성은 유사한 경향으로 5% 울금의 급여가 감소시킨 것으로 확인되었다. SD계 수컷 흰쥐의 혈청 LDH 활성은 BS군에서 유의적인 차이를 나타내며 높은 활성을 보였으나(p<0.05), 5% 울금의 급여가 감소 효과를 나타내었다(p<0.05). 카탈라아제 활성은 BS군에서 유의적으로 낮은 활성을 나타내었고, ST군에서 활성을 증가시켰다(p<0.05). 혈청 아밀라아제 및 리파아제의 활성은 5%　울금의 급여가 감소시킨 것으로 확인되었다. 본 연구 결과, SD계 수컷 흰쥐를 통한 in vivo 실험에서 5% 울금의 섭취가 당뇨, 내인성 항산화 효소 및 간 기능 개선에 효과가 있는 것으로 나타났다.

울금(Curcuma longa L.)이 이상지질혈증 유발 흰쥐의 지질성분과 단백질 및 전해질 농도에 미치는 영향을 확인하기 위하여 기본 식이를 섭취시킨 ND군, 정상 실험군에 5% 울금 급여군(NT군), 이상지질혈증 유발 실험군(DD군)과 DD군에 5% 울금을 섭취시킨 실험군(DT군)으로 나누어 실험을 행하였다. 혈청 지질성분(총 콜레스테롤, LDL-콜레스테롤, 중성지질, 인지질, 유리 콜레스테롤, 콜레스테롤 에스테르), 혈당 및 유리지방산(non esterified fatty acid, NEFA)의 농도와 동맥경화지수 (atherosclerotic index, AI), 심혈관 위험지수(cardiac risk factor, CRF)는 이상지질혈증군에서 여타 실험군과 비교하였을 때 유의적인 증가를 나타내었으며(p<0.05), 5% 울금 급여군에서 감소시키는 것으로 나타났다. DD군에 비해 DT군에서 고밀도 지단백 콜레스테롤(high density lipoprotein-cholesterol, HDL-콜레스테롤) 및 총 콜레스테롤에 대한 HDL-콜레스테롤 농도 비는 유의적인 증가를 보였다 (p<0.05). 혈청 단백질의 농도에 있어서, DT군은 DD군에 비하여 흰쥐의 혈청 알부민(albumin) 농도와 알부민/글로불린 비(albumin/globulin ratio, A/G 비)의 증가 및 글로불린(globulin) 농도는 감소되는 것으로 관찰되었다. 나트륨(Na) 및 염소(Cl)는 DT군에서 DD군 보다 농도가 감소된 것으로 확인하였다. 이상의 결과, 5% 울금은 흰쥐의 혈청 지질성분 개선과 단백질 및 전해질 농도 조절에 도움이 되는 것으로 사료된다.

울금(Curcuma longa L.)의 산화억제 및 질소산화물 소거활성 등 기능성 소재로서 활용 가능성을 검토한 결과, 프로안토시아니딘(proanthocyanidin) 함량은 69.000±2.737 mg catechin equivalents (CE)/g dry weight으로 확인되었으며, 증류수(distilled water, DW), 70% 에탄올 및 노르말 부탄올(n-butanol)의 3가지 용매를 사용한 추출 수율은 DW (17.11%), 70% 에탄올(15.26%), 노르말 부탄올(4.12%) 순으로 관찰되었다. 총 플라보노이드(total flavonoid) 함량은 DW, 70% 에탄올 및 노르말 부탄올에서 각각 0.032, 0.512 및 2.221 mg quercetin equivalents (QE)/g의 함량으로 나타났고, 노르말 부탄올에서는 유의적인 차이를 보이며 높게 관찰되었다(p<0.05). Nitric oxide (NO) 라디칼 소거 활성은 농도 별(0.2~0.8 mg/mL) DW에서 15.64~26.20%, 70% 에탄올 10.52~20.76%, 노르말 부탄올 에서 13.39~69.92%로 확인되었다. Nitrite (NO2) 소거활성은 DW 및 70% 에탄올과 비교하였을 때 노르말 부탄올에서 강한 NO2 소거활성을 보였다. β-carotene 탈색 저해활성은 DW에서 8.81~25.93%, 70% 에탄올 1.20~20.20%, 노르말 부탄올 12.08~43.93%로 나타났다. 지질과산화 저해활성은 DW, 70% 에탄올 및 노르말 부탄올에서 각각 5.60~27.54%, 37.78~50.79% 및 41.79~46.39%로 동정되었다. 이에, 천연 항산화제 등 기능성 소재로서의 활용 가능성이 있을 것으로 사료된다.

This study was conducted to evaluate the effects of adding gradually increasing concentrations of turmeric extract (0, 0.1, 0.2, and 0.4%) to smoked duck meat on its chromaticity, antibiosis, and antiseptic degree against food poisoning bacteria, number of bacteria, lipid rancidity, sensory evaluation, and preference. The brightness, red color intensity and yellow color intensity changed significantly when 0.2% turmeric extract was added and the sodium nitrate concentration was reduced. Additionally, no antibiosis or antiseptic activities against food poisoning bacteria were observed in any turmeric treated samples, whereas the number of bacteria was increased in control samples compared to turmeric treated samples after 10 days of preservation. The TBARS value decreased during storage when turmeric extract concentration increased, resulting in positive sensory evaluation of its color, succulence, taste and hardness. In the preference test, the surface and meat color increased as the concentration of turmeric extracts increased. Hardness was highest at 0.2%, whereas taste was highest between 0.2 and 0.4%. The overall preference test was highest for the 0.2% extract samples. Overall, the results indicated that addition of 0.2% turmeric to smoked duck meat will lead to better nutrition, function, and overall preference.

울금(Curcuma longa L.)의 사포닌 함량과, 증류수(distilled water, DW), 70% 에탄올 및 노르말 부탄올의 3가지 용매를 사용하여 산화억제 효과를 통한 기능성 식품 소재로서의 가치를 확인한 결과, 총 사포닌 함량은 7.506±0.349 mg SE/g dry weight으로 확인되었다. 추출 수율은 DW (17.11%), 70% 에탄올(15.26%), 노르말 부탄올(4.12%)로 DW에서 가장 높게 나타났으며, 노르말 부탄올 추출물에서 낮은 수율을 보였다. 항산화 활성은 농도가 높아질수록 활성이 증가되는 것으로 관찰되었다. 총 페놀 함량은 DW, 70% 에탄올, 노르말 부탄올 순으로 각각 8.57, 41.01, 119.11 mg GAE/g의 함량으로 나타났으며, 노르말 부탄올 추출물에서 유의적인 차이를 보이며 높게 관찰되었다(p<0.05). DPPH 라디칼 소거활성은 DW, 70% 에탄올, 노르말 부탄올 순으로 확인되었다. ABTS 라디칼 소거활성, 환원력 및 ferric reducing antioxidant power (FRAP) 또한 DPPH 라디칼 소거활성과 비슷한 양상으로 나타났지만, ferrous ion 킬레이트 능은 노르말 부탄올(15.87~21.17%), 70% 에탄올(26.10~50.76%), DW(44.47~84.24%) 순으로 측정되었다. 이에, 울금은 산화억제 및 생리활성을 가지는 것으로 규명되었으며 기능성 식품 소재로서의 활용 가능성이 높은 것으로 사료된다.

Nosema disease caused by the microsporidia Nosema apis and Nosema ceranae are a honey bee pathogen parasitizing. Nosema disease symptoms include digestive and absorption disorders because the spores damage epithelial tissue and potentially causing colony death. Recently, N. ceranae has been reported as an important threat to honey bee health. Turmeric (Curcuma longa L.) Curcuma tonga L. belongs to the family Zingiberaceae and is a perennial, tropical herb. Turmeric, the powdered rhizome, is used for medicinal purposes. The aim of this study was to evaluate the potential of Turmeric (Curcuma longa L.) for the control of N. ceranae in honeybees. For the study, we infected with N. ceranae spore through dosed and fed with the turmeric extraction at difference concentration. The data show that the turmeric extraction was not toxic for bee at least at 1% and the bees fed with 0.5 % turmeric extraction had significantly lower infection rates. This data suggest that turmeric could be useful in alternative strategies for the control of N. ceranae.

The purpose of this study was to improve the nutrition and the permeability of functional plants by using cryogenic grinding technology. Barley sprouts, Curcuma longa L., Dendropanax morbifera LEV., Phellinus linteus were dried, ground and extracted in different temperature conditions. Powder size of barley sprouts and Curcuma longa L. were about 50 μm and Dendropanax morbifera LEV. and Phellinus linteus were about 20 μm. Cryogenic ground of Barley sprouts preserved 18.27-124.65% of nutrients such as protein, ash, carbohydrate, beta carotene, minerals, vitamins. Cryogenic grinding powder of Curcuma longa L. show high nutrients retention rate of lipid and carbohydrate. Permeability was measured by Parallel Artificial Membrane Permeability Assay (PAMPA) to predict passive gastrointestinal absorption. Permeability of saponarin, which is marker compound of Barley sprouts, is 9.88 times higher in cryogenic grinding powder than ambient grinding powder. Curcumin permability is 3.1 times higher than ambient grinded powder. As a result, particle size, nutrition, protein digestion degree and permeability demonstrated a positive relationship with the decreasing grinding temperature for the powders. These results confirm that the cryogenic grinding method had good suitability to increase functionality of plants, since it could minimize the heat generated while processing and effectively reduce the particle size.

The antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from turmeric (Curcuma longa L.) was investigated in this study. The antioxidant potential of various extracts of turmeric was evaluated by using different antioxidant tests, namely DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ABTS [2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, ferric reducing antioxidant power and β-carotene bleaching assays as well as total phenol content and total flavonoid content. All extracts exhibited abundant total phenol content, total flavonoid content and strong antioxidant activity. Particularly, higher activity was exhibited by CM extract with 46.32±0.35 mg CAE/g and 15.58±0.39 mg QE/g of total phenol content and total flavonoid content. Generally, antioxidant activity of various extracts from turmeric increased in the following order: 70% methanol extract, 70% ethanol extract and CM extract, respectively. Results suggested that turmeric could be widely used as a source of antioxidant and a kind of functional material in food.

The repellent activities of 33 plant extracts against Aedes albopictus were examined using a patch test for adult. The six plant extracts (Magnolia denudate, Dryopteris crassirhizoma, Cnidium officinale, Lindera obtusiloba, Magnolia kobus, and Houttuynia cordata) showed over 70% repellency activities to Ae. albopictus. The insecticidal activity of Curcuma longa against Ae. albopictus was also determined. C. longa hexane extraction showed 100% larvicidal activity at 1,000 ppm after treated 24 h. Purification of the biologically active constituents from the hexane extraction with larvicidal activity was done using silica gel column chromatography. H1 fraction gave 100% mortality to Ae. albopictus at 100 ppm. H12 fraction was determined 87.8% larvicidal activity to Ae. albopictus at 50 ppm. Active constituent was analysed as the sesquiterpene, ar-turmerone (C15H20O) by GC and GC-MS. C. longa extract gave highly protection against mosquito bites. The hexane extraction showed complete protection at 10 mg over 6 h. In this study, the sesquiterpene from C. longa induced a protection time, repellency, landing time, and biting time against Ae. albopictus. These results suggest that the some plant extracts and C. longa have the potential to be used an eco-friendly materials for the control of mosquitoes.

The insecticidal activities of materials derived from the rhizomes of turmeric, Curcuma longa L., against third instar larva of Culex pipiens and Aedes albopictus were evaluated using a direct contact mortality bioassay. Curcuma longa L. hexane extraction showed 100% larvicidal activity both two mosquitoes species at 1,000 ppm after treated 24 h. Purification of the biologically active constituents from the hexane extraction with larvicidal activity was done using silica gel column chromatography. H1 fraction gave 100% mortality to C. pipiens and A. albopictus at 100 ppm. H12 fraction was determined 100% and 87.8% larvicidal activity to C. pipiens and A. albopictus at 50 ppm, respectively. H12 fraction was analyzed as the sesquiterpene, ar-turmerone (C15H20O) and turmerone(C15H22O) by GC and GC-MS.

The purpose of this study was to determine the optimal mixing conditions for two different amounts of turmeric (Curcuma longa L.) powder and olive oil for the processing of chicken sausage. The experiment was designed according to the central composite design of response surface methodology, with ten experimental points, including two replicates for turmeric powder and olive oil. The physicochemical and mechanical analysis of each sample, including water holding capacity, moisture content, lightness, redness, yellowness, hardness, chewiness, gumminess, and cohesiveness, showed significant differences. The results from sensory evaluations also showed very significant differences in color, flavor, tenderness, chewiness, and overall quality. The optimal formulation, calculated by numerical and graphical methods, was 1.89 g of turmeric powder and 9.77 g of olive oil. Under these conditions, the model predicted pH-6.01, salinity-0.20, WHC-94.88, L* value-61.13, b* value-37.45, hardness-36.66×102 (N), springiness-8.70 (mm), chewiness-26.88×103 (N×mm).

This study was conducted to investigate rheological and sensory characteristics of fish paste prepared with Curcuma longa L. powder (0, 1, 3, 5, 7%). The moisture content of the fish paste ranged from 71.35 to 72.97%. Increasing the amount of Curcuma longa L. powder in the fish paste tended to decrease the pH value and the folding test good score in all samples. Increasing the amount of Curcuma longa L. powder in the fish paste also tended to decrease the lightness (L) in the hunter color value, but to increase the redness (a) and yellowness (b). For the textural characteristics, the addition of Curcuma longa L. powder increased the strength and decreased the springiness. In a sensory evaluation, the addition of 3% Curcuma longa L. powder had the best score in taste and overall preference. Therefore, this results suggest that adding 3% Curcuma longa L. powder would be feasible for making Curcuma longa L. fish paste.

This study was conducted to investigate the quality characteristics of the natural dishwashing liquid added with the mixture extracts of Curcuma Longa L. (CL), Morus alba (MA) and Ecklonia cava (EC) at various concentrations. The pH and lightness of the natural dishwashing liquid were reduced by adding the mixture extracts, while the turbidity was increased. The natural dish washing liquid added with mixture extracts was shown to display strong antimicrobial activities against L. monocytogenes compared to that of control. Also, it revealed that antioxidant activity was increased depending on concentrations. However, natural dishwashing liquid added with mixture extracts showed the low detergency efficiency. In sensory evaluation, the natural dishwashing liquid containing CL 0.5%, MA 0.25% and EC 0.25% was preferred than the control and it showed negative result in skin patch test. These results suggest that the addition of CL 0.5%, MA 0.25% and EC 0.25% positively improved the qualities characteristics in the natural dish washing liquid.

Curcumin, the major yellow-colored pigment in turmeric(Curcuma longa L.), was extracted by using supercritical carbon dioxide. Optimum extraction conditions were determined. Overall experiments were planned by central composite design and results were analyzed by response surface methodology to find effects of three independent variables, temperature(X1), co-solvent flow rate(X2) and pressure(X3) on the yield of curcumin extract(Y). Regression model optimized by response surface analysis was as follows Y = -8.581270 + 0.220770X1 + 1.176731X2 + 0.036873X3 + -0.0026816X12 - 0.013010X2X1 - 0.103353X22 + 0.000198X3X1 - 0.0000825X3X2 - 0.000096554X32. Optimum temperature, pressure and co-solvent flow rate for extracting curcumin from turmeric were 40.31oC, 3.07 ml/min and 231.59bar, respectively, and statistical maximum yield of curcumin was 1.922%.

The purpose of this study was to investigate the improvement effect of 5% turmeric (Curcuma longa L.) on the blood glucose and lipid metabolism function of streptozotocin (STZ, 45 mg/kg body weight)-induced diabetic rats. Seven-week-old male rats were divided into four groups (n=6), and fed experimental diets containing turmeric meal [basal diet+5% turmeric (BT), basal diet+STZ+5% turmeric (ST)], and control (BD), BS groups (basal diet+STZ). The results of this study, turmeric diet groups (BT, ST) in lipid component as evidenced from the significantly reduction of serum total cholesterol, low density lipoprotein-cholesterol (LDL-cholesterol), atherosclerotic index (AI), cardiac risk factor (CRF), triglyceride (TG), phospholipid (PL), free cholesterol, cholesteryl ester, uric acid, blood glucose, non esterified fatty acid (NEFA), and elevation of high density lipoprotein-cholesterol (HDL-cholesterol). The serum albumin and globulin contents were increased in turmeric supplementation diet than STZ-induced diabetic rats (p<0.05). Concentrations of sodium (Na) and chlorine (Cl) in sera were lower in the turmeric diet than diabetic group. Total calcium (T-Ca), phosphorus (Pi) and potassium (K) concentrations in sera were higher in the BT, ST and BD groups than BS group. In vivo experiments with Sprague-Dawley rats showed that ingestion of turmeric (Curcuma longa L.) were effective in the blood glucose and lipid metabolism functional improvement.

The purpose this study was to investigate the influences of 5% turmeric (Curcuma longa L.) supplementation on enzyme activities such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), amylase, lipase and catalase in serum of dyslipidemic rats. Sprague-Dawley (SD) rats (24 male) were divided into four groups, namely the ND group (normal-nondyslipidemic diet), NT group (normal-nondyslipidemic diet+5% turmeric), DD group (control-dyslipidemic diet), and DT groups (dyslipidemic diet+5% turmeric). Serum concentrations of blood urea nitrogen (BUN), creatinine and uric acid were significantly decreased (p<0.05) by turmeric supplementation diet. The activities of AST, ALT, ALP, LDH, amylase and lipase in sera of turmeric diet group were significantly decreased (p<0.05). The catalase activity in serum of turmeric supplementation group was significantly increased than dyslipidemic diet (p<0.05). In vivo experiment with dyslipidemic rats showed that ingestion of turmeric were effective in kidney and hepatic functional enzyme activities. Which suggests that turmeric material could be used for further studies as a potential source for nutraceutical foods.