본 연구는 국내에서 아직 식품색소로 지정되지 않은 zeaxanthin과 citranaxanthin에 고성능 액체 크로마토그래피 (HPLC)를 적용하여 최적화된 동시 분석법을 개발하고, 이 분석법을 검증하기 위해 수행되었다. 최적의 분석법을 확립하기 위해, 기존에 보고된 다양한 국외 문헌 시험법의 HPLC 분석법을 비교 검토하였다. 그리고 분석 조건 선정 시 선형성, 검출한계(LOD), 정량한계(LOQ), 정확성, 반복 성, 실험실 간 검증 등의 주요 검증 항목을 고려하여 다 양한 식품에 적용 가능한 최적 전처리법을 개발하였다. 개 발된 분석법은 우수한 선형성(R20.999)을 나타내었으며, 식품 매트릭스 내에서 zeaxanthin과 citranaxanthin에 대한 검 출한계(LOD)는 각각 0.11-0.32 mg/kg 및 0.14-0.20 mg/kg, 정량한계(LOQ)는 각각 0.33-0.96 mg/kg 및 0.42-0.60 mg/kg 으로 확인되었다. 기존 연구에 보고된 전처리 방법은 다 양한 식품군에 적용하는 데에 한계를 보였으나, 본 연구 에서 개발된 분석법은 카로티노이드 착색료가 사용되는 주요 식품군에서 모두 적용이 가능하였다. 특히 이 방법 은 적용성 평가에서 zeaxanthin과 citranaxanthin에 대해 모 든 식품군에서 90% 이상의 우수한 동시 회수율을 나타내 었다. 또한, 새로운 HPLC 분석법을 적용한 두 물질의 정 확도(회수율) 및 정밀도(%RSD)는 식품의약품안전처(MFDS) 가이드라인에서 제시한 기준 범위에 부합하였다. 따라서 본 연구에서 개발된 동시 분석법은 식품 중 zeaxanthin과 citranaxanthin을 신뢰성 있고 정확하게 분석할 수 있는 방 법으로 판단되었다.

This study examined the simultaneous application of absorption and adsorption by adding granular activated carbon to tap water (Absorbent-A) and sodium hypochlorite (Absorbent-B), with the aim of improving the removal performance of gaseous acetaldehyde applying hybrid process. Prior to the main experiment, preliminary tests were conducted to assess the effects of HOCl concentration (50~150 ppm) and reactor temperature (25~35oC). In the main experiment, the removal performance under activated carbon-added conditions was compared using the concentration ratio (Co/ Ci) and its rate of change over time. As a result, the addition of activated carbon led to a more gradual saturation and concentration change compared to the non-addition condition, with the addition showing the most stable trend. This study suggests a basic reference for improving the performance of conventional absorbents enhancing the durability and efficiency with the activated carbon as an auxiliary material for future field applications.

Lumpy Skin Disease (LSD) and Foot-and-Mouth Disease (FMD) cause substantial economic losses on the livestock industry. Therefore, vaccinations have been implemented as the control strategy in endemic countries. However, the potential adverse effects of administering vaccines for both diseases simultaneously have not been thoroughly evaluated. The aim of this study was to assess the impact of vaccinating dairy cows with either or both LSD and FMD vaccines on milk production and physiological parameters such as milk temperature, rumination time and body weight. The experimental groups were divided into four according to the injection materials: 1) saline, 2) LSD vaccine, 3) FMD vaccine, and 4) both vaccines. The impact of vaccination on milk yield and physiological parameters was evaluated daily until 12 days post-vaccination, and milk components were analyzed twice, once per week. Among the experimental groups as well as each vaccine group, no statistically significant differences (p < 0.05) were observed at milk yield, milk components, or milk temperature. This suggests that simultaneous vaccination of LSD and FMD can be administered without adverse effects.

딤프로피리다즈는 pyrazole carboxamide 계열 살충제로 활성형은 TRPV 상류 부위를 표적으로 하여 신호전달을 차단하며 진딧물 등의 해충 방제에 효과적이다. 사이클로 뷰트리플루람은 phenethyl pyridineamide 계열 살충제로 미 토콘드리아 전자 수송 사슬복합체 II의 호흡작용을 저해 한다. 딤프로피리다즈와 사이클로뷰트리플루람에 대한 국 내 잔류물의 정의는 모화합물이며, 각각 수박, 호박잎 등 29품목에 대하여 0.03-7.0 mg/kg, 배추 등 6품목에 대하여 0.03 mg/kg으로 잔류허용기준이 설정되어 있다. 본 연구 에서는 농산물 중 딤프로피리다즈와 사이클로뷰트리플루람 의 잔류량을 분석하기 위한 공정시험법을 마련하고자 하였 다. 딤프로피리다즈 및 사이클로뷰트리플루람을 분석하기 위하여 아세토니트릴을 추출용매로 사용하는 QuEChERS법 을 이용하여 추출 및 정제조건을 확립한 후, LC-MS/MS 를 분석기기로 선정하였다. 딤프로피리다즈 및 사이클로 뷰트리플루람의 결정계수(R2)는 모두 0.99 이상으로 우수 하였으며, 정량한계는 0.01 mg/kg으로 나타났다. 대표 농 산물 5종(현미, 감자, 대두, 감귤, 고추)에 대하여 정량한 계, 정량한계 10배, 정량한계 50배 수준으로 처리하여 회 수율 실험을 한 결과 딤프로피리다즈의 평균 회수율은 85.5-101.5%, 사이클로뷰트리플루람은 86.8-108.5%을 보였 으며, 변이계수(coefficient of variation, CV)는 각각 10.9%, 13.7% 이하로 나타났다. 본 연구의 결과는 국제식품규격 위원회 농약 분석법 가이드라인25)의 잔류농약 분석 기준 및 식품의약품안전평가원의 식품 등 시험법 마련 표준절 차에 관한 가이드라인24)에 적합한 수준이다.

In this study, an simultaneous LC-MS/MS multi-residue analytical method was developed and validated for the residues of six neonicotinoid insecticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam) in honey. Sample preparation included a combination of QuEChERS extraction kit and liquid-liquid extraction method to effectively extract pesticide components from the honey matrix and optimized analytical conditions to achieve high sensitivity and selectivity. The limits of detection (LOD) and the limits of quantitation (LOQ) were set in the range of 6-15 ng/mL and 19-44 ng/mL, respectively and the correlation coefficient (R²) was greater than 0.99, confirming good linearity. In addition, the intra-day recoveries for each pesticide were 75-104%, and the coefficient of variation (CV) was less than 20%, which met the guideline recommended by the Ministry of Food and Drug Safety. The LC-MS/MS method developed in this study is expected to be used as a multi-residue analysis method for 6 neonicotinoid pesticides in honey.

본 연구에서는 2023년 전라남도 내 유통 수산물 중 동 물용의약품 모니터링을 하였다. 수산물 124건에 대하여 동 물용의약품 136종을 식품공전 수산물 중 동물용의약품 동 시 다성분 시험법으로 모니터링 하였다. 검사결과 잔류허 용기준 이하로 검출된 시료는 16건(13%), 검출되지 않은 시료는 108건(87%)으로 나타났다. 동물용의약품이 검출된 수산물은 광어, 조피볼락, 도미, 미꾸라지 4종으로, 검출된 잔류동물용의약품은 엔로플록사신, 옥시테르라사이클린, 트리메토프림, 아목시실린이 각각 검출되었다. 동물용의약 품 잔류허용기준이 초과한 수산물은 없었으나 동물용의약품이 잔류한 수산물이 꾸준히 검출되고 있어 안전한 수산 물 유통을 위해서는 모니터링을 강화하여야 할 것으로 생 각된다.

Gold nanoparticles (Au NPs) decorated carbon nanofibers (CNFs) have been prepared by an electrospinning approach and then carbonized. The prepared Au-CNFs were employed to modifying a screen printed electrode (SPE) for simultaneous determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Au NPs are uniformly dispersed on carbon nanofibers were confirmed by the structure and morphological studies. The modified electrodes were tested in cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry (CA) to characterize their electrochemical responses. Compared to bare SPE, the Au-CNFs/SPE had a better sensing response to AA, DA, and UA. The electrochemical oxidation signal of AA, DA and UA are well separated into three distinct peaks with peak potential separation of 280 mV, 159 mV and 439 mV between AA-DA, DA-UA and AA-UA respectively in CV studies and the corresponding peak potential separation in DPV studies are 290 mV, 166 mV and 456 mV. The Au-CNFs/SPE has a wide linear response of AA, DA and UA in DPV analysis over the range of 5–40 μM ( R2 = 0.9984), 2–16 μM ( R2 = 0.9962) and 2–16 μM ( R2 = 0.9983) with corresponding detection limits of 0.9 μM, 0.4 μM and 0.3 μM at S/N = 3, respectively. The developed modified SPE based sensor exhibits excellent reproducibility, stability, and repeatability. The excellent sensing response of Au-CNFs could reveal to a promising approach in electrochemical sensor.

In this study, hybrid devices were developed to simultaneously remove odor and particulate matter (PM) emitted during meat grilling, and their performance was evaluated. A ceramic filter system and surfactant microbubble plasma system were used to reduce particulate matter. For odor reduction, an electro-oxidation system, an ozone-active catalytic oxidation system, and a multi-adsorption filter system were used. By combining the above particulate matter reduction and odor reduction devices, the reduction efficiency of odor and particulate matter generated during meat grilling was analyzed. As a result, most of the six combined device conditions showed a reduction efficiency of more than 90% for particulate matter. The combined odor also showed a high reduction efficiency of less than 200 times the emission concentration standard. This study also evaluated 22 types of odorous substances, of which ammonia (NH3) and hydrogen sulfide (H2S) showed removal efficiencies of more than 99%. Therefore, it is expected that the combination of these technologies can be used and applied directly to the sites where meat grilling restaurants are located to effectively contribute to the simultaneous reduction of particulate matter and odor.

본 연구는 그린커피빈추출물이 「건강기능식품의 기준 및 규격」에 추가로 등재될 경우를 대비하여 표준화된 클 로로겐산 시험법을 설정하고, 카페인이 동시 분석되도록 최적화하는 연구를 진행하였다. 최적화된 시험법을 마련 하기 위해 기기분석 및 전처리 조건을 비교·분석하여 클 로로겐산과 카페인을 30% 메탄올 추출하여 인산용액과 인산 함유 아세토니트릴으로 액체크로마토그래프를 통해 330 nm, 280 nm에서 분석하도록 시험법을 설정하였다. 시 험법 밸리데이션 결과, 직선성 정량범위 내에서 상관계수 (R2) 0.999 이상의 유의수준을 보였고, 클로로겐산과 카페 인 검출한계는 0.5와 0.2 μg/mL, 정량한계는 1.4와 0.4 μg/ mL로 나타났다. 정밀도와 정확도 결과는 AOAC 밸리데 이션 가이드라인를 통해 적합함을 확인하였고, 클로로겐 산 및 카페인 동시분석법을 최종적으로 마련하였다. 또한, 시제품과 유통제품을 통해 제형별 적용성 검토하여 클 로로겐산과 카페인을 동시에 정량 가능한 시험법임을 재확인하였다. 최적화된 시험법은 클로로겐산을 함유한 건강기능식품 품질관리에 대한 신뢰성을 더 높일 것으 로 본다.

The recent increase in the occurrence of common bed bug and tropical bed bug in shared areas highlights the need for rapid species identification at infestation sites, which is crucial for implementing targeted control measures due to differences in genetic and physiological traits. In this study, molecular diagnostic methods were developed using species-specific ITS2 sequences. Both multiplex PCR and loop-mediated isothermal amplification (LAMP) protocols with a DNA release method successfully distinguished between the two bed bug species regardless of developmental stages in 0.5~2.5 hours, even with dead specimens. Especially, LAMP's simplicity and speed make it applicable for rapid and accurate bed bug diagnosis at infestation sites.

Achieving cost-effective and defect-free graphene sheets is highly desirable for sensor devices. Aiming this, few-layer graphene (~ 3) sheets are prepared by an electrochemical exfoliation with [NMP] [ HSO4] electrolyte (i.e., Bronsted acidic ionic liquid). A novel approach for the effective exfoliation of graphene sheets is demonstrated by (i) simultaneously applying a constant potential through an electrochemical cell (with different electrolyte concentrations) and (ii) together with sonication. The exfoliated graphene sheets are characterized through state-of-the-art techniques and sprayed on a glass substrate at optimum conditions. Thus, the transparent conducting sensor device is fabricated with a suitable contact electrode and used for ammonia vapor sensing and the sensor performances are highly dependent on the concentration of the ionic liquid used during the electrochemical exfoliation. The sensing response and limit of detection for the exfoliated graphene-based film were calculated as 3.56% and 432 ppb, respectively. Further studies indicated that the fabricated sensors are more selective towards ammonia molecules with quick response and recovery times.

A glassy carbon electrode modified with a composite consisting of electrodeposited chitosan and carboxylated multi-walled carbon nanotubes (e-CS/MWCNTs/GCE) was used as a working electrode for simultaneous determination of dopamine (DA), serotonin (5-HT) and melatonin (MT), which were related to circadian rhythms. The electrochemical characterizations of the working electrode were carried out via electrochemical impedance spectroscopy and chronocoulometry. It was found that electrochemical modification method, that was cyclic voltammetry, may can cause continuous CS polymerization on MWCNTs surface to form a dense membrane with more active sites on the electrode, and the electrochemically active surface area of e-CS/MWCNTs/GCE obtained was about 7 times that of GCE. The electrochemical behaviour of DA, 5-HT and MT on working electrode were carried out via differential pulse voltammetry and cyclic voltammetry. The results showed that e-CS/MWCNTs/GCE solved the problem that the bare electrode could not detect three substances simultaneously, and can catalyze oxidation potential difference as low as 0.17 V of two substances reaction at the same time, indicating very good electrocatalytic activity. By optimizing the detection conditions, the sensor showed a good linear response to DA, 5-HT and MT in the range of 20-1000 μmol/L, 9-1000 μmol/L and 20-1000 μmol/L, and the detection limits were 12 μmol/L, 10 μmol/L and 22 μmol/L (S/N = 3), respectively. In addition, the proposed sensor was successfully applied to the simultaneous detection of DA, 5-HT and MT in human saliva samples.