This study investigated the antioxidant activities of wine made with aronia (Aronia melanocarpa). The ethanol concentration of the aronia wine was increased up to 7.8±0.1% on the 8th day of fermentation. Compared to other types of wine, the total amount of organic acids was highest in raspberry wine, followed by grape wine, arona wine, and aronia juice. Because, acetic, oxalic, and succinic acids were not detected in the aronia juice, but were detected in the aronia wine, it was determined that they were produced during alcohol fermentation. The polyphenol content in the aronia juice and wine was higher than in the grape wine and raspberry wine and was twice as much in the aroni wine than in the aronia juice. The flavonoid content in aronia juice and wine was higher than in commercial grape wine and raspberry wine. The DPPH radical scavenging ability was higher than 50% in the aronia wine and juice samples. ABTS radical scavenging activity was higher in aronia juice and wine than in raspberry wine and grape wine. The results of this study suggest that the development of wine with high antioxidant activity is possible if wine is made with aronia.

This study is to establish optimizing condition of alcohol fermentation in vinegar production with aronia, to confirm whether this can be industrially used, and to compare and analyze a change in anti-oxidative activity and quality characteristic according to alcohol fermentation of aronia. The optimized conditions for alcohol fermentation were as follows: Saccharomyces cerevisiae 5645 of yeast strain, a 5% inoculum size, aronia juice with a brix value of 14, and a glucose content of 7%. As a result to conduct scale up with optimizing conditions of alcohol fermentation of aronia, 8 days (192 hrs) of total alcohol fermentation time and 7.4% of the final alcohol content. The harvest volume accounted for approximately 90.2% with a loss of about 2.8%. As a result of antioxidant test, anti-oxidative activity of alcohol fermented liquor is lower than anti-oxidative activity of aronia extract, because of the decrease of antioxidant by oxidation of the fermentation process. However, the decrease of tannin by the fermentation process reduces acerbity of aronia, so increases overall preference

After making kimchi by adding 0, 0.5, 1.0, 1.5% aronia (Aronia melanocarpa), microbiological and sensory characteristics were measured while fermenting it at 10oC for 28 d. The numbers of total cell and lactic acid bacteria were decreased after the maximum levels of microbial counts; that is, day 3 for control and 0.5% treatment and day 14 for 1.0, 1.5, and 2.0% treatments. Coliforms were not detected after 7 d in control and 0.5% treatments; however, they were detected after 14 d i-n 1.5 and 2.0% treatments throughout the fermentation period. Sensory properties were evaluated in the aspects of both acceptability and intensity characteristics. In the preference characteristics, overall acceptability was highest in the 1.0% group after preparation and after 14 d of fermentation. As for the aronia-added group, the intensity of the hot taste of Nabak Kimchi decreased while that of its sweet taste and sour taste increased according to the rise of both the amount of the added aronia to the kimchi and fermentation period. In conclusion, the addition of 1.0% aronia can be used to produce high-quality Nabak Kimchi while extending its storage period.

This study examined the effects of drying methods of Aronia melanocarpa on contents of total polyphenols and flavonoids, antioxidant activity and anti-inflammatory activity. As a result, freeze-dried aronia extract (FDAE) significantly exhibited higher contents of total polyphenols and flavonoids (155.76 mg GAE/g and 105.70 mg QE/g) than hot-air dried aronia extract (HDAE) (134.93 mg GAE/g and 82.29 mg QE/g). Also, FDAE showed greater antioxidant activity than HDAE in both DPPH and ABTS. For anti-inflammatory activity, NO production from lipopolysaccharide activated RAW264.7 cell reduced at a dose-dependent manner in both FDAE and HDAE. However, reduction rate of NO production is higher in FDAE (62.7%) than in HDAE (33.5%). These results suggest that comparatively, freezedrying is a better method for preserving the antioxidant and anti-inflammatory activities and of aronia.

Aronia is difficult to eat because it has a bitter taste, bitter taste, and sour taste. Because processing is essential for eating, quality information suitable for processing is needed. This experiment was carried out to confirm the step suitable for processing. Four grades of ripening were collected from 3 varieties (Viking, Nero, McKenzie) based on color, and compared with samples stored for 2 weeks after harvesting. Physicochemical properties such as color, hardness, sugar content, pH and acidity, functional components such as total polyphenols and flavonoids, and antioxidative activities such as DPPH and ABTS were investigated. When compared according to varieties, the hardness of the first degree was highest in other degrees, Depending on harvest time, first degree was higher than other degrees and it decreased with harvest time. Varieties was not significant different lightness, redness and yellowness value. compared according to harvest time, redness was the highest in second degree and lightness, yellowness decreased with harvest time. sugar content was highest in Nero and Mckenzie, birx value increased significantly of ripening and increased with harvesting time. pH was found to be highest in Nero, acidity value was the highest in viking. DPPH, ABTS radical scavening were Nero varieties had the highest radial scavening and increased significantly as the ripening degree increased. Total phenol and flavonoid contents were found to be highest in Nero, the highest content was showed at the first degree of harvesting. The most suitable stage for the processing was considered to be the best after post harvest treatments considering brix, hardness and acidity.

Aronia makgeolli was manufactured using Asp. kawachi (AK) and Asp. awamori (AA) with tannase activity, and physicochemical properties were examined during the fermentation period. The pH was decreased during the fermentation period after the first day, while the acidity increased. Reducing sugars increased highly on the first day of fermentation, and then they gradually decreased as the fermentation period elapsed. On the 7th day of fermentation, it was in the range of 0.38~0.61%. The alcohol content gradually increased during the fermentation period and it ranged from 13.4~14.2 v/v% by the 7th day of fermentation, and the alcohol content of makgeolli added with Aronia was somewhat lower than that of makgeolli prepared without aronia. The L value increased as the fermentation period elapsed, and the L value of makgeolli added with aronia increased rapidly. The a value gradually decreased, while the b value gradually increased as the fermentation period elapsed. The content of total polyphenols increased during the fermentation period of AK makgeolli. However, the AA makgeolli was not significantly increased, as compared to the initial stage of fermentation, and it was lower than that of the AK makgeolli. The radical scavenging activity of the DPPH was higher in the makgeolli added with aronia, and the antioxidant activity of AA makgeolli was higher than that of AK makgeolli. In the sensory evaluation, in the AK makgeolli, the palatability deteriorated due to the bitter taste and the astringent taste derived from the aronia. However, in the AA makgeolli, astringent taste was very weak and the sensory quality was good.

We examined the quality characteristics of pound cake with prepared Aronia melanocarpa powder, which included the physical characteristics, antioxidant activity and sensory evaluation in this study. Pound cakes were prepared with various ratios of Aronia powder (0, 5, 10, 15, 20%). No significant difference in loss rate or weight was found from increased Aronia powder concentration. The color of pound cake was darker and reddish with the addition of Aronia powder. Lightness (L) and yellowness (b) significantly decreased with the addition of Aronia powder, whereas redness (a) increased (p<0.05). the qualities of hardness, cohesiveness, gumminess and chewiness of the pound cake prepared with the addition of Aronia powder were significantly increased when compared to control(p<0.05), with exception of 20% addtion. Total phenolic contents and DPPH radical scavenging activity was significantly elevated by the addition of Aronia powder(p<0.05). The pound cake with 10% Aronia powder showed the strongest positive sensory properties with regard to taste and overall palatibility. These results suggest that the addition of 10 % Aronia to pound cake batter could well serve to improve the overall quality, texture and taste of the final product.

Fludarabine, a chain terminating anti-cancer drug, is a purine analogue that causes DNA strand breaks in normal cells. In this study, we determined if A. melanocarpa and Korean red ginseng extract mixture reduce cytotoxicity of fludarabine. Treatment of HaCaT cells with 10 μM of fludarabine for 24 hours decreased cell viability and increased DNA strand breaks. Treatment of A. melanocarpa and Korean red ginseng extract mixture for 24 hours increased cell viability as compared with single extract treatment. The protective effect of these extracts on cell activity increased in a concentration-dependent manner. DNA strand breaks induced by fludarabine decreased as concentration of extract mixture increased. p-H2AX level, a marker of DNA strand breakage, decreased depending on the concentration of extract mixture. The effect of mixed extract of A. melanocarpa and Korean red ginseng on DNA damage is due to the anti-oxidative effect of A. melanocarpa and signal transmission through glucocorticoid receptor upon binding of saponin of Korean red ginseng.

The nutritional compositions and physicochemical properties of two aronia varieties (Viking and Nero) cultivated in Chungbuk were investigated. The proximate compositions of Nero and Viking powder were:13% moisture, 0.58% and 0.6% ash, 0.73% and 0.33% crude lipid, 5.47% and 6.51% crude protein, 15.65% and 16.15% crude dietary fiber, and 64.36 and 64.43% crude carbohydrate, respectively. The proximate contents of the aronia samples were not significantly different. Among the physicochemical properties, there was also no significant change in total anthocyanin and antioxidant activity. However, Viking had higher total polyphenol contents and α-glucosidase inhibition activity than Nero. The mineral contents of Al, Fe, Mn, Mg, Ca, Na, K, Cu and Zn in the two aronias were determined. Cu and Zn were not detected in both varieties. With regard to Al, Na and K, Nero was higher than Viking, while Ca was higher in Viking than Nero. From the results, Viking could be suggested to be beneficial for food processing.

본 연구는 막걸리 제조 시 아로니아의 첨가 방법을 달리하 여 제조한 후, 발효하면서 항산화 활성 및 이화학적 품질특성 을 분석하였다. 아로니아는 생과와 마쇄한 형태로 첨가하였 고, 쌀 함량의 10%, 30% 그리고 50%로 하였다. 막걸리의 pH 는 발효 초기에 생과를 첨가한 시료가 3.9~4.2로, 마쇄하여 첨 가한 시료(3.6~3.9)보다 높았고, 발효가 진행되면서 생과로 첨가한 경우는 pH가 낮아지다가 다시 증가하는 경향성을 보 였다. 총산의 경우는 초기 발효에는 생과보다 마쇄한 시료에 서 더 높게 나타났고, 발효가 진행될수록 총산의 함량이 증가 하였다. 환원당 함량은 발효가 진행되며 대부분의 시료에서 감소하였다. 에탄올 함량은 1단 담금 시 11.4%로 나타났고, 2단 담금 6일 후에는 10~15%였으며, 특히, 아로니아를 50% 첨가한 실험구는 1단 담금 시 보다 낮은 에탄올 함량을 보였 으며, 마쇄하여 첨가할수록 더 낮은 알코올함량을 보였다. 색 도 중 a값(적색도)을 비교했을 때 생과 사용 시 천천히 a값이 높아져 마지막 날에는 생과 처리구 간 유의적으로 차이가 났 지만, 마쇄한 경우는 처음부터 마지막 발효까지 높은 a값을 가져 생과를 처리한 경우보다 높게 나타났다. 총 폴리페놀함 량 측정과 DPPH radical 소거능 측정 결과는 아로니아 첨가량 이 증가할수록 함께 증가하였는데, 생과첨가군의 경우 초기 에는 낮은 값을 가지다가 발효가 진행하며 급속히 증가하였 고, 마쇄첨가군에서는 초기에서부터 높은 값을 가졌다. 또한 아로니아를 30% 첨가하여도 50%를 첨가한 것과 같이 뛰어난 항산화 활성을 나타내었다. 관능검사는 생과 30%를 첨가 한 시료가 색, 쓴맛, 전체적 기호도에서 높아 관능적 만족도 가 우수한 것으로 나타났다. 이상의 결과로 아로니아를 첨가 한 기능성 막걸리의 특성을 보았을 때 아로니아를 마쇄한 형 태보다 생과 형태로 30%를 첨가하는 것이 관능적 특성과 항 산화 활성을 고려하였을 때 가장 적합한 것으로 나타났다.

Background : Aronia melanocarpa ‘Viking’ known as black chokeberry, is a species of rosaceae family. Chokeberry is high in flavonoid/anti-oxidants as well as high in vitamins and minerals. Black chokeberry contains high amount of polyphenols, especially anthocyanins. Methods and Results : The aim of this study is to figure out the antioxidant and anti-inflammatory property of Aronia melanocarpa ‘Viking’ (AMV) for both water and ethanol extract. The extracts have been tested to assess the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2ʹ-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and nitrite scavenging activity. We have also evaluated the reducing power activity of AMV. AMV extracts showed a good radical scavenging activity on DPPH and ABTS free radical. The DPPH radical scavenging activity ranges from 20.59% - 80.12% and 26.12% - 95.59% for water and ethanol extract, respectively. The total phenolic and flavonoid contents have also been estimated in this study. Moreover, to appraise the anti-inflammation property we examined cell viability and inhibition of nitric oxide (NO) production by using the lipopolysaccharide-treated RAW 264.7 macrophage cell line. The extracts showed no cytotoxic effects on RAW 264.7 cells. Conclusion : According to the results, we suggest AMV contains noticeable antioxidant properties and could be source of natural antioxidant substances.

본 연구는 아로니아를 이용한 다양한 가공식품의 개발에 대한 일환으로 튀김어묵 제조에 아로니아의 기능성 성분을 활용하고자 아로니아 착즙액을 첨가하여 튀김어묵을 제조한 후 품질특성을 조사하여 기능성 식품 개발을 위한 기초 자료를 제공하고자 하였다. 아로니아 착즙액을 물 양 대비 0, 2, 7, 12%를 첨가한 어묵을 제조한 다음 일반성분, 산도, 당도, pH, 색도, 절곡검사, 조직감, 관능적 특성을 평가하였다. 첨가구의 일반성분은 대조구와 차이는 없었으며, 시판어묵과는 유의적인 차이가 있었다(p<0.05). 시판어묵, 대조구 및 첨가구의 산도는 0.10-0.11%, 당도는 2.77-3.10 °Brix로 첨가구의 경우 아로니아 착즙액의 첨가량이 증가함에 따라 높아지는 경향이었고, pH는 6.44-7.11으로 아로니아 착즙액의 첨가량 증가에 따라 감소하는 경향을 나타내었다. 첨가군의 L 값과 b 값은 첨가량이 증가함에 따라 낮아졌으며, a 값(redness)은 높아지는 경향을 나타내었다. 절곡검사 결과는 아로니아 착즙액의 첨가유무와 첨가량에 관계없이 모두 AA로 측정되었고, 어묵의 조직감은 강도(strength), 경도(hardness)와 씹음성(chewiness)의 경우 아로니아 착즙액의 첨가량이 증가함에 따라 높아졌으며, 시판어묵과 대조구는 유사하게 측정되었다. 응집성(cohesiveness)은 시판어묵, 대조구 및 첨가구간에 차이는 나타나지 않았다. 총 폴리페놀 함량 측정결과 시판어묵과 대조구는 비슷하게 측정되었고, 첨가군은 착즙액의 첨가량에 비례하여 증가하였다. DPPH radical 소거능 측정결과 대조구에 비해 첨가구의 IC50 값이 낮게 측정되어 아로니아 착즙액의 첨가량이 증가할수록 항산화활성도 증가하는 것으로 나타났으며, 시판어묵의 경우 12% 첨가구와 유사한 DPPH 소거능을 나타내었다. 관능평가는 색, 향, 조직감, 맛, 전반적인 기호도 평가에서 7% 첨가구와 시판어묵이 높은 점수를 받았다. 이상의 결과를 통하여 아로니아 착즙액을 첨가하여 튀김어묵을 제조할 때 첨가하는 아로니아 착즙액의 양은 7%가 가장 적합할 것으로 사료된다.

Background : The objective of this study was to investigate antioxidant activities, inhibitory activities against heme induced colonic epithelial cell proliferations, anti-inflammatory activities and anthocyanin profiles in the anthocyanin rich fraction (ARFAM) from fruits of Aronia melanocarpa, where these are considered functional substances and available food coloring agents in Korea. Methods and Results : Anthocyanins were identified by reversed-phase C18 column chromatography and HPLC-DAD-ESI/MS analysis. To compare the antioxidative and anti-inflammatory capacity of Aronia melanocarpa berries, recognized for their high content of anthocyanins, isolation method was developed to obtain high-purity anthocyanins in the extract. Anthocyanin-rich fractions (ARFAM) enriched in anthocyanins were found to be potent strong inhibitory activity towards heme induced colonic epithelial cell proliferations are associated with an increased risk of colon cancer than acidic ethanol extract (AME). The immunomodulation properties were assessed in growth of both human B and T cells, its cytokines secretion such as IL-6 (interleukin-6) and TNF-α (tumor necrosis factor-alpha). AME enhanced interleukin-6 and reduced tumor necrosis factor-a production, whereas ARFAM only had a effect in increasing of IL-6 expression. Conclusion : These results demonstrated that there was no major relationship between the antioxidative and immunomodulation capacities of AME and ARFAM.

Background: This study represents the first report that the anti-obesity activity of ethanol extracts of Aronia melanocarpa can be enhanced through ultrasonification at a frequency of 120 ㎑ at 60℃ (UE). Methods and Results: The amounts of cyanidin-3-O-galactose (cya-gal), a major anthocyanin in A. melanocarpa were higher by up to 402.4 ㎎/100 g, as compared with 221.4 ㎎/100 g and 322.1 ㎎/100 g, for hot water at 100℃ and 70% ethanol at 80℃ respectively. This result should cause the higher antioxidant activities of the UE than extract of hot water and ethanol in DPPH free radical scavenging. It was confirmed that the high antioxidant activity of UE could play an important role in inhibiting the production of proteins related to adipocyte differentiation, such as peroxisome proliferator activated receptor-γ (PPAR-γ) and sterol regulatory element binding protein 1 (SREBP1). Conclusions: Ultrasonification at a frequency of 120 ㎑ at 60℃ should result in better anti-obesity activity than that observed using other processes. It was also observed for the first time that the anti-obesity activity of A. melanocarpa was associated with its antioxidant activity, possibly due to the higher elution of intact cya-gal, owing to efficient low temperature ultrasonification extraction. These results could also be applied to improve other biological activities of medicinal herbs that contain many types of heat-labile bioactive substances.

The purpose of this study was to measure the antioxidant activity and bioactivity of black chokeberry (Aronia melanocarpa). The black chokeberry was known to contain many physiologically active substance, such as ascorbic acid, anthocyanin, quercetin. We extracted black chokeberry powder from four solvents such as chloroform:methanol(CM, 2:1, v/v), distilled water (DW), 70% ethanol, 70% methanol. After that, we determined anthocyanin, total phenol, flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, ferric reducing antioxidant power, reducing power and Nitrite scavenging activity of black chokeberry extracts and as results of comparing each extract. respectively. From the above results shows that antioxidant activity and bioactivity of black chokeberry extracts was the highest in 70% methanol (p<0.05). The results suggest that black chokeberry can be used as nutraceutical foods and natural antioxidant.

아로니아의 활용성 제고를 위하여 건조방법에 따른 품질 특성을 비교한 결과, 건조과정 중 중량변화율은 진공동결 건조가 81.6%로 가장 많은 중량감소를 나타내었고, 수분함 량은 진공동결건조에서 가장 많은 수분이 감소하였으며, 건조 후 최종 수분함량은 진공동결건조에서 0.99%로 가장 낮은 함량을 나타내었다. 색도 측정결과, L*값은 건조방법 에 따른 큰 차이를 나타내지 않았으며, a*값과 b*값은 진공 동결건조와 냉풍건조에서 증가하였고, 열풍건조에서는 감 소하였다. 경도는 열풍건조에서는 건조 36시간 이후 급격 하게 증가하였고, 냉풍건조는 서서히 증가하다가 132시간 이후 급격하게 증가하는 모습을 보였다. 건조방법에 따른 품질특성으로 건조수율은 냉풍건조가 24.20%로 가장 높았 으며, 열풍건조 20.40%, 진공동결건조 19.41% 순으로 나타 났다. 일반성분은 생과에서 수분이 61.76%로 가장 높게 나타났으며, 조회분은 열풍건조에서 가장 높게 나타났고, 가용성무질소물, 조단백, 조지방은 진공동결건조에서 가장 높은 함량을 나타내었다. 건조방법에 따른 아로니아의 pH 는 3.84~3.92로 큰 차이가 없었으며, 당도는 동결건조 (6.57°Brix) > 열풍건조(5.37°Brix) > 냉풍건조(4.40°Brix) > 생과(1.07°Brix) 순으로 나타났다. 무기질 함량은 진공동 결건조에서 가장 높았으며, 주요 무기질은 K, Ca, Mg, Na인 것으로 나타났다. 아로니아에 함유된 당은 fructose, glucose 두 종류인 것으로 나타났으며, 진공동결건조가 20.59 g/100g으로 가장 높은 함량을 가진 것으로 나타났다.

Antioxidant activity is important for reducing oxidative stress that causes various metabolic disorders. Metabolic disorders are highly related to loss of productivity in livestock. Therefore, development of effective antioxidant compounds originating from plants is important for organic agriculture. Phenolic compounds in edible plants are regarded as major components relevant to antioxidant activity. The present study investigated the changes in antioxidant activity and phenolic compound profiles of Aronia (Aronia meloncarpa) by fermentation using different strains of Leuconostoc mesenteroides. A total of 5 strains of L. mesenteroides were used as starter cultures and their β-glucosidase activities were measured. A total of 6 experiment runs were prepared, one for control (uninoculated) and the others (inoculated) for treatments. For biological activity, antioxidant and antibacterial activities were measured. For phenolic compound profiling, TLC and HPLC analysis were performed. The strains of KACC12313 and KACC12315 showed greater enzyme activity than others. Treatment with KCCM35046 showed strong and broad antibacterial activity against to Listeria monocytogenes. Treatments with KCCM35046 and KACC12315 showed the highest total polyphenol content. The highest antioxidant activity was found in KACC12315 treatment. No remarkable alteration was found in thin layer chromatography (TLC) analysis. In phenolic compound profiling analysis, KCCM35046 showed notable alteration in compound area ratio compared to others and also showed the highest caffeic acid content. In chlorogenic acid, treatments with KCCM35046 and KACC12315 showed great content than others. Treatment with KACC12315 showed the greatest content of trans-ferulic acid. As a result of relative performance indexing analysis, L. mesenteroides KCCM35046 and KACC12315 were selected as the best strain for the fermentation of Aronia.

This study was indicated to enhance the anti-inflammation activities by the fermentation of the fruits of Aronia melanocarpa (Michx.) Elliott. The extracts by 70% ethanol (EE) showed better biological activities than those by hot water (WE) from campared result of the effect of extraction solvents. Then, the extract from 70% ethanol extraction was further fermented by lactic acid, denoted as FEE. For antioxidant activities, the FEE had showed the highest value as 0.832 of reducing powder, in comparison with those of EE and WE. Cytotoxicity of the water extraction (WE) was measured for 12.06% in addition of 1.0mg/mℓ of FEE. For anti-inflammation activities, NO production from the macrophage, RAW 264.7 was observed as 7.24μM and 8.52μM from FEE and EE, respectively. Prostaglandin E2 (PGE2) production from human fibroblast cell, CCD-986sk, was also estimated for 152pg/mℓ in addition of 1.0mg/mℓ of the FEE. The lowest production of both IL-6 and TNF-α were 3.5pg/mℓ and 865.5pg/mℓ, respectively in addition of 1.0mg/mℓ of the FEE, whereas 74.5pg/mℓ and 982.4pg/mℓ in treated with same concenrations of the EE. It was also found that the FEE was higher amounts than other extracts through HPLC analysis of the anthocyanins. These results strongly indicate that fermentation process of the lactic acid could enhance anti-inflammation activities of extracts by increasing the amounts of the anthocyanins, especially cyanidin-galactoside. Our results suggest that the application of the fermentation process for other medicinal herbs can be improved their biological activities.