This study investigated the volatile flavor components of the essential oil from Chrysanthemum coronarium var. spatiosum Bailey. The essential oil obtained from the aerial parts of the plant by the hydrodistillation extraction method was analyzed by gas chromatography and gas chromatography-mass spectrometry. One hundred and one (99.11%) volatile flavor components were identified in the essential oil from the Chrysanthemum coronarium var. spatiosum Bailey. The major compounds were hexanedioic acid, bis(2-ethylhexyl) ester (12.45%), 6.10.14-trimethyl-2-pentadecanone (7.94%), 1-(phenylethynyl)-1-cyclohexanol (6.34%), α-farnesene (5.55%), phytol (4.99%), and α-caryophyllene (4.39%). When the volatile flavor components of Chrysanthemum coronarium var. spatiosum Bailey were classified by functional group, the content was high in the order of hydrocarbons, alcohols, esters, ketones, aldehydes, and phthalides. Sesquiterpene hydrocarbons were the most common hydrocarbons, mainly due to α-farnesene and α-caryophyllene. Among the alcohols, the content of aliphatic alcohols was significantly higher, mainly due to 1-(phenylethnyl)-1-cyclohexanol (6.34%) and phytol (4.99%). The analysis of the volatile flavor components of Chrysanthemum coronarium var. spatiosum Bailey in this study will provide useful information to consumers when purchasing food and to industries using fragrance ingredients.

Eco-friendly materials, such as alternative vegan materials using various fungal resources, are being actively researched to reduce environmental pollution and facilitate a healthy lifestyle. The fungal mycelium-based mushroom mycelium mat is one such emerging material. In this study, the commonly used mushroom mycelium culture method was modified to reduce the time required to produce the mycelium mat, lower the possibility of contamination, and improve the properties and quality of the mat. Shortening the period required for the previously used primary bag culture and secondary mat production culture. A culture method in which the bag culture was omitted was attempted using a mycelium mutated by gamma irradiation to the mycelium of Trametes orientalis. In addition, various nutrients were added to the fungal solution to observe the change in physical properties of the fungal mat. High-quality mycelium mats were produced in the experimental group containing 1.5% CaCO3 in sawdust medium, and the period was also reduced by more than 10 days compared to the existing production method. In the future, for mass producing mycelium mats, additional selection of medium components and optimization of culture conditions are essential.

The objectives of this study are to increase the contents of functional compounds and antioxidant activity obtained from ginger (Zingiber officinale Roscoe) extracts using fermentation and enzyme. The optimal conditions (1%, Aspergillus luchuensis) for extraction methods were determined through fermentation treatment of ginger (concentrations of 0.5 and 1%; Aspergillus oryzae and Aspergillus luchuensis). The quality characteristics of the ginger extract for each treatment method (HW, hot water treated; EZ, enzyme; AL, fermentation; ALEZ, fermentation and enzyme) were observed using the optimal conditions. The water-soluble index (WSI) and total sugar content of the ALEZ increased by 2.6 times and 6.5 times, respectively, compared with that of the HW. However, the sum of gingerol and shogaol contents of ALEZ were 73.9% in ratio compared with lower than that of the HW. Antioxidant contents were generally higher in ALEZ, similar to the WSI trend. Therefore, ALEZ was more effective in enhancing antioxidant activity than EZ and AL. The fermentation and enzymatic approach described in this study would be beneficial to food industries for developing ginger functional products and materials.

Radioactively contaminated metal components from a nuclear power plant must be decontaminated to reduce the risk of radiation exposure to workers, which can be cleaned using a foam decontamination used to reduce the amount of wastewater significantly. Metal components with a fixed radioactive contamination can be effectively decontaminated using a foam consist of 0.5wt% nonionic surfactant, 0.5 M H2SO4, and 0.2 M Ce(SO4)2. However, strongly acidic wastewater is generated from the decontamination method, which contains a high concentration of the nonionic surfactant and ionic materials with radioactive nuclides. This wastewater must be treated as a stable form. In this study, an integrated process of precipitation and low pressure distillation was evaluated for the treatment of wastewater. It was confirmed that the surfactant and ionic materials were effectively removed from the wastewater through the integrated process.

The permanent shut-down of Kori unit 1, the first nuclear power plant (NPP) in Korea, generates various radioactive waste. They are dry active waste (DAW), spent resin, concentrated waste, activated metals, etc. During normal operation of NPP, activated metals are rarely generated. The decommissioning of NPP, however, generates massive amount of metallic waste including activated metals and contaminated metals, while normal operation generates small amount of metallic waste. The reactor vessel and internals are relatively highly activated components in NPP. Since they are exposed to the high concentration of neutrons during the operation, their contains relatively high radioactive nuclides. They activation analysis is usually performed to understand the radiological inventory of the activated reactor vessel and internal. The results offer various important information including, radiological inventory, waste classification, etc. The impurities in the carbon steel and stainless steel have a great impact on the radiological inventory of the activated metals. The cobalt, nickel, niobium are primary elements that affects the activation analysis and waste classification. Especially, the cobalt, which transforms to 60Co, plays an important role. The 60Co, strong gamma emitter, affects the waste classification, safety analysis of decommissioning workers, and determination of segmentation and package plans. In this paper, effects of impurity concentration on activation analysis is studied. The expected impurity from various sources, including NUREG/CR-3474, commercial NPP data, etc, and effects will be demonstrated. Also, the comparison between results and international experiences will be followed.

It can take hundreds of thousands of years for decreasing radiological effects of high-level radioactive wastes to those of natural background radiation. Therefore, long-term time scale should be considered in order to demonstrate performance and safety of deep geological disposal of the radioactive wastes. The changes of surface environment for these long-term time scale can have influence on safety analysis by changing transport path of radionuclides from the radioactive wastes. Changes in climate is considered as one of main factors causing the long-term changes of the surface environment. The own effects and interactions of climate with other components of the geological disposal system are organized in features, events, and processes (FEPs). In this study, some natural processes occurred by changes of climate were suggested and the connectivity between each process is proposed based on the relation of the FEPs concerned with the changes of climate and surface environment. The processes were classified into global and regional/local scales and was analyzed, respectively. Then, the influences of the processes on shallow groundwater and surface water body environment, which might be transport path of radioactive nuclides in local/site scales, were expected. As the proposed connection demonstrate the order or hierarchical relations of the natural processes, it can shows that some output by a certain process may be input of other process connected the former process in numerical simulations for interpreting the processes. If the connection may be considered to be suitable to represent longterm changes of the surface environment, it can be evaluated that the expected scenarios based on the connection is also proper. In addition, it can be helpful in selecting factors to be studied more detailed in terms of climate change for expecting long-term changes in the surface environment by analysis on the input and output data. The results of this study can be used as basic approaches to represent the long-term changes in the surface environment caused by specific natural processes from changes of climate. It will be also helpful for formulating scenarios related to long-term evolution in the surface environment required for performance and safety assessments of the deep geological disposal.

Recently, an unprecedented emerging infectious disease has rapidly spread, causing a global shortage of wards. Although various temporary beds have appeared, the supply of wards specializing in infectious diseases is required. Negative pressure isolation wards should maintain their function even after an earthquake. However, the current seismic design standards do not guarantee the negative pressure isolation wards’ operational (OP) performance level. For this reason, some are not included in the design target even though they are non-structural elements that require seismic design. Also, the details of non-structural elements are usually determined during the construction phase. It is often necessary to complete the stability review and reinforcement design for non-structural elements within a short period. Against this background, enhanced performance objectives were set to guarantee the OP non-structural performance level, and a computerized tool was developed to quickly perform the seismic design of non-structural elements in the negative pressure isolation wards. This study created a spreadsheet-based computer tool that reflects the components, installation spacing, and design procedures of non-structural elements. Seismic performance review and design of the example non-structural elements were conducted using the computerized tool. The strength of some components was not sufficient, and it was reinforced. As a result, the time and effort required for strength evaluation, displacement evaluation, and reinforcement design were reduced through computerized tools.

Seismic demand on nonstructural components (NSCs) is highly dependent on the coupled behavior of a combined supporting structure- NSC system. Because of the inherent complexities of the problem, many of the affecting factors are inevitably neglected or simplified based on engineering judgments in current seismic design codes. However, a systematic analysis of the key affecting factors should establish reasonable seismic design provisions for NSCs. In this study, an idealized 2-DOF model simulating the coupled structure-NSC system was constructed to analyze the parameters that affect the response of NSCs comprehensively. The analyses were conducted to evaluate the effects of structure-NSC mass ratio, structure, and NSC nonlinearities on the peak component acceleration. Also, the appropriateness of component ductility factor (R p) given by current codes was discussed based on the required ductility capacity of NSCs. It was observed that the responses of NSCs on the coupled system were significantly affected by the mass ratio, resulting in lower accelerations than the floor spectrum-based response, which neglected the interaction effects. Also, the component amplification factor (a p) in current provisions tended to underestimate the dynamic amplification of NSCs with a mass ratio of less than 15%. The nonlinearity of NSCs decreased the component responses. In some cases, the code-specified R p caused nonlinear deformation far beyond the ductility capacity of NSCs, and a practically unacceptable level of ductility was required for short-period NSCs to achieve the assigned amount of response reduction.

As a language writing symbol inherited by the Chinese nation for nearly a thousand years, Chinese characters have obvious ideographic characteristics, and the components of Chinese characters play an important role at the ideographic level. Different components are combined with each other to jointly participate in the overall formation meaning of Chinese characters. Therefore, the interpretation of the shape and meaning of Chinese characters is naturally inseparable from the study of components. After the development and evolution of Chinese characters in the past dynasties, both the overall shape and the partial components of the characters have changed over time. Modern Chinese characters use common regular script. If we trace and explore its specific components in combination with past dynasties, it is not difficult to find that the meaning and source of the component itself may be more than one. In modern Chinese characters, there are a large number of Chinese characters with component “口”. In most cases, the component “口” plays the formation meaning function of “mouth”. However, if we trace the origin of the Chinese characters containing the component “口” and start from the early forms of the relevant Chinese characters, we can discover a variety of formation meaning functions of the component “口” except "mouth". Through research, this paper finds that the modern Chinese character component “口” not only means “mouth”, but also has other formation meaning functions such as “ware”, “symbol” and “range”. For the research on the origin of the formation meaning of the Chinese character component “口”, the related academic achievements still need to be improved. This paper has the significance value of exploring and improving the multiple functions of the specific components.

The aim of study to investigate the phytochemicals and biological activities the bark of Betula schmidtii. The studies consisted of the solvent extraction, followed by the isolation of phenolic components 1~3 from ethyl acetate-soluble fraction of Betula schmidtii Bark. Their chemical structures were identified as arbutin (1), ρ-coumaric acid (2) and ferulic acid (3) using Ultraviolet-Visible (UV-Vis) Spectrophotometer, Electrospray Ionization Mass Spectrometry (ESI-MS) (negative ion mode), 1H-Nuclear Magnetic Resonance (NMR), 13C-NMR, 1H-1H Correlation Spectroscopy (COSY) and 1H-13C Hetero Nuclear Multiple Quantum Correlation (HMQC) spectral data. Compounds 1~3 shows the anti-oxidant effect with IC50 values of 29.74±1.52, 21.32±1.07 and 34.41±1.24 in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, respectively. Also, compounds 1~3 exhibited mushroom tyrosinase inhibitory activity with IC50 values of 31.14±1.07, 42.54±1.46 and 69.22±1.43 μM, respectively.

Seismic fragility analysis of a structure is generally performed for the expected critical component of a structure. The seismic fragility analysis assumes that all the components behave independently in a structural system. A bridge system consists of many inter-connected components. Thus, for an accurate evaluation of the seismic fragility of a bridge, the seismic fragility analysis requires the composition of probabilities considering the correlation between structural components. This study presented a procedure to obtain the seismic fragility curve of a bridge system, considering the correlation between bridge components. Seismic fragility analysis was performed on a PSC bridge that is considered as the central infrastructure. The analysis results showed that the probability of the seismic fragility curve of the bridge system was higher than that of each bridge component.

The optimal determination of seeding rate is critical to minimizing uncertainties about the large variations observed in forage quality and productivity when alfalfa is cultivated under different geographical areas and growing conditions. The objective of this investigation was to provide information about the proper seeding rate according to harvest timing for alfalfa cultivation in the Northern regions of Korea. Alfalfa was sown in September 2018 at a seeding rate of 20, 30 or 40 kg/ha and harvested four times in 2019: May 3, July 2, September 11, and October 13. Regardless of seeding rate, alfalfa plant height was longest at the third harvest (113 cm) and the shortest in the last annual harvest (43.8 cm). However, seeding rate had no effect on alfalfa plant height at any harvest. Forage relative feed value was increased in the first cutting but decreased in the third cuttings as seeding rate increased. However, seeding rate had slight effect on alfalfa forage quality components at the second and fourth cuttings. Total annual DM and crude protein production (in 4 harvests) was greater at higher seeding rates. Plots seeded at a rate of 40 kg/ha produced on average 1,257 and 2,620 kg/ha more forage (DM basis) than those seeded at a rate of 30 or 20 kg/ha, respectively. Forage DM production at the first, second, third, and fourth harvests accounted for 36.1, 24.0, 27.1, and 12.8 % of total annual DM production, respectively. Overall, small differences were seen when alfalfa seeding rate was different but maximum forage DM production (in four harvests) was detected when seeding rate was 40 kg/ha. These data could be useful to the alfalfa growers by allowing them to make more accurate trade-offs between seed price and the expected magnitude of forage yield gains in order to select the best seeding rate.

본 실험에서 미세플라스틱의 노출은 넙치의 혈액학적 성상인 hemoglobin 및 hematocrit 수치의 유의적인 감소를 유발하였다. 또한 미세플라스틱의 노출은 넙치 혈장 무기 성분인 calcium, 유기성분인 glucose 및 cholesterol, 효소성분인 AST의 유의적인 변화를 나타냈다. 본 연구에서 바이오플락 환경에서 미세플라스틱의 노출이 어류의 혈액생리에 영향을 주며 독성으로 작용하는 것을 확인할 수 있었다. 향후, 일반 해수와 바이오플락 환경으로 각각 양성한 넙치를 이용하여 사육환경의 변화에 의한 미세플라스틱 노출 영향의 차이에 대해서도 추가적인 연구가 이루어져야 할 것이다. 본 연구는 다양한 어종에서 종별 미세플라스틱의 농도 연구에서 미세플라스틱 노출에 따른 종별 독성영향 비교평가를 위한 기본 자료로 활용될 수 있을 것이다. 하지만, 바이오플락 환경에서 미세플라스틱의 응집은 미세플라스틱의 독성에 영향을 미치는 중요한 요소이기 때문에 (Choi et al. 2020), 향후 연구에서 철저한 모니터링과 함께 다양한 미세플라스틱 독성에 미치는 요소에 대한 추가연구가 필요할 것이다.

본 연구에서는 기존에 인체에 유익한 효과가 있고 부작 용 없이 장기간 안전하게 사용할 수 있으며 간보호 및 간 기능 개선에 탁월한 효과가 있는 표고를 추출 및 농축하여 원유에 농도별로 첨가하여, 3종의 유산균을 이용하여 발효하였다. 표고의 농도를 달리하여 유산균을 배양하였을 때 면역관련 물질인 ergothioneine 및 β-glucan의 함량 을 측정한 결과 ergothioneine함량은 30% 표고추출농축액에 L. plantarum이 첨가되어 배양된 발효물이 40.48 mg/100 g로 가장 높은 함량을 나타냈다. β-Glucan은 30 % 표고추출농축액에 L. brevis가 첨가된 발효물이 13.94 %로 가장 높은 함량이 나타났다. 두 면역관련 물질 모두 표고추출농축액 첨가량이 증가할수록 증가하는 경향을 나타냈다. SD rat을 간을 적출하여 일차 간세포로 분리해 발효물의 간보호 효과를 측정하였다. 표고추출농축액과 유산균 발효물을 10, 50, 100, 500 μg/mL을 일차 간세포에 처리 하여 MTT assay 방법으로 세포독성을 측정한 결과, 모든 농도에서 독성을 보이지 않았다. 또한 10 mM 아세트아미 노펜을 처리하여 독성을 유발한 일차 간세포에 시료를 처리하여 AST, ALT, LDH를 측정한 결과, 효소의 농도가 감소하여 간보호 기능에 효과가 있음을 확인하였다. 이중 L. brevis를 첨가하여 배양한 발효물 처리구에서 효소의 농도가 가장 감소한 것을 보았고, 가장 간보호 효과가 뛰어난 것을 확인하였다. 이상의 결과를 토대로 표고추출농축액에 유산균을 첨가하여 발효하였을 때, 기존의 표고보다 면역관련 물질의 함량이 증가하고, 간기능 보호에 더욱 효과가 있음을 입증하였다. 따라서 본 연구결과는 표고 유산균 발효물이 건강기능 소재로서 연구를 위한 기초 연구자료로 활용될 수 있 것으로 생각된다.

표고버섯은 톱밥배지 재배기술의 발달로 세계적으로 가장 많이 생산되고 식용되는 버섯이며, 톱밥배지를 이용하여 재배된 표고버섯의 생산량이 늘고 있다. 본 연구에서는 톱밥재배 표고버섯의 품종별 식품학적 차이를 비교하고자 동일 조건에서 재배된 산조701호, 산조707호, 산조715호, 참아람, L808 총 5품종 표고버섯의 일반성분, 영양성분 및 항산화 활성을 분석하였다. 동결건조된 표고버섯의 일반성분 함량은 회분 4.06~5.92 g/100 g, 조지방 0.75~1.02 g/100 g으로 품종별 차이가 크지 않았으나 조단백질은 21.24~29.15 g/100 g으로 품종간의 유의적 차이를 보였다. Trehalose는 산조701호에서 9.60±0.08 g/100 g 으로 가장 높은 함량으로 확인되었다. 총 아미노산 조성은 glutamic acid>aspartic acid>leucine 순으로 높은 분포를 보였으며, 유의적 차이를 보인 아미노산은 glycine, alanine, valine, isoleucine, leucine, phenylalanine이었으며 산조707 호, 산조715호, 산조701호, 참아람, L808 순으로 높은 함량을 보였다. 물 추출물의 총 폴리페놀 함량과 총 플라보노이드 함량은 산조715호가 3.49±0.04 mg GAE/g, 1.33±0.03 mg QE/g으로 가장 높게 나타났으며 다른 품종 들과 유의적 차이를 나타내었다. 같은 톱밥배지와 환경에 서 재배된 표고버섯 5 품종간의 유의적 차이가 확인된 성분은 조단백질, trehalose, 6종의 아미노산이었다. 하지만 한 곳의 임가에서 재배된 표고버섯 품종간의 비교 결과로, 품종별 시료 수를 확대한 추가실험을 통하여 품종 간의 구별 가능성을 더욱 세부적으로 검토할 필요가 있다.

This study investigated on the nutritional components and physiological activity of four wild vegetables namely Salvia plebeia R. Br., Angelica acutiloba, Gynura procumbens and Saururus chinensis Baill for the development of representative wild vegetables in Chungbuk. Salvia plebeia possessed the highest radical scavenging activity and beta-carotene, but exhibited the lowest α-glucosidase inhibitory activity compared to the other three vegetables. Angelica acutiloba showed high crude protein content and α-glucosidase inhibitory activity, but contained low total polyphenol content, radical scavenging ability and beta-carotene compared to the other three vegetables. Gynura procumbens showed high mineral content, beta-carotene, vitamin K1 content and α-glucosidase inhibitory activity, but showed the lowest total polyphenol content and radical scavenging ability compared to the other three vegetables. Saururus chinensis showed high crude fiber content and total polyphenol content, but contained the lowest mineral and vitamin K1 content. To conclude, it is suggested to use Salvia plebeia or Saururus chinensis as antioxidant food materials and Angelica acutiloba and Gynura procumbens as food materials and sources of α-glucosidase inhibitors. In particular, it is believed that Saururus chinensis, which possessed high content of crude fiber, is suitable for low-calorie food materials such as diet products.

특허발명의 일부 구성요소만을 실시하는 경우 에도 간접침해가 인정될 수 있다. 그런데 특허발 명의 일부 부품을 수출하여 완성품이 해외에서 조립되는 경우에도 간접침해가 성립할 수 있는지 문제된다. 미국은 Deepsouth Packing 사건 이후 법적 허점이 드러났다는 비판을 감안하여 미국 특허법 제271조(f)를 입법함으로써, 간접침해가 성립하도록 하였다. 반면, 독일 및 일본에서는 미 국과 같은 규정이 없으며, 이 두 나라 모두 부품 을 국내에서 생산하고 외국에서 완성되는 경우, 간접침해를 인정하지 않는다. 독일의 경우 독일 특허법 제10조에 특허발명의 실시 장소를 독일이 라고 규정하는 반면, 일본의 경우 법원의 해석에 따라 완성품의 실시 장소를 일본 국내라고 보았 다. 우리나라의 경우 대법원 2014다42110 판례 에 따라 완성품의 생산 장소를 국내로 보았다. 따 라서 국내에서 생산되는 수출용 부품에 대해 간 접침해로 규제할 수 없게 되었다. 이러한 판례는 현행 우리 특허법 규정, 그리고 독일 및 일본과 같은 다른 나라의 제도를 고려할 때 타당한 결론 이라고 보인다. 다만 특허권을 손쉽게 회피하는 문제를 방지하면서도, 특허권의 과도한 확장은 방 지하도록 적절한 균형점을 찾는 것이 중요하다. 이러한 관점에서 특허법 개정이 필요해 보인다