폐양액 발생을 최소화할 수 있는 배액제로형 수경재배기술 개발을 위하여 토마토 수경재배 시 배액제로 센서를 이용하여 배액을 제로화 또는 최소화하였을 때 표준배액량 처리와 비교하여 근권환경 변화와 토마토의 생육, 수량, 품질 등에 미치는 영향을 구명하였다. 처리별 주당 1일 공급량은 표준배액률 처리가 1.4, 배액제로 1처리가 0.9, 배액제로 2처리가 0.8L이었고, 배액률은 표준배액률 처리가 23.8, 배액제로 1처리가 8.6, 배액제로 2 처리가 3.7%이었다. 표준배액률 처리, 배액제로 1과, 2처리의 함수율과 배지 내 EC는 각각 64.5~88.0%와 1.5~3.5dS·m-1, 40.3~76.0%와 2.5~4.0dS·m-1, 그리고 56.3~69.0%, 2.7~3.7dS·m-1이었다. 토마토 생육은 표준배액률 처리에 비해 배액제로 처리에서 엽장, 엽수, 경경은 차이가 없었으나, 초장과 엽폭이 작은 경향을 보였다. 토마토 수량은 표준배액률 처리에 비해 배액제로 처리에서 상품수량이 7.7~12% 적고 1과중이 작았으나, 당도는 반대로 높았고 상품과률은 차이가 없었다.

시설 내 PPFD는 오전에는 남북동이, 오후에는 동서동이 높았다. 일평균 PPFD는 동서동이 높았는데, 이는 태양광의 입사각이 작아질 때 동서동의 수광면적이 증가되기 때문인 것으로 조사되었다. 고랑위 60cm 높이의 PPFD는 전 고랑에서 남북동이 동서동보다 높았으나 수량과는 관련이 없었다. 평균 기온은 동서동이 높았으나 2월중순 이후로는 태양고도가 높아짐에 따라 차이가 없었다. 지온은 동서동이 다소 높았고 이랑 간에 차이가 없었다. 과실수량은 동서동이 8% 많았는데, 이는 남쪽이랑의 과실이 수확기가 빨라서 초기수량이 많았기 때문이다. 동계 단동형 비닐하우스를 이용한 토마토 촉성재배는 반촉성재배와는 달리, 동서동 시설을 이용하는 것이 유리하였다.

전남 서남부지역의 참다래 과수원에서 발생하는 과숙썩음병원균(fruit rot, Botryosphaeria dothidea)에 대한 항균작용이 우수한 세균성 균주를 선발하기 위하여 참다래 과수원 토양으로부터 단일균주를 분리하였으며, 과숙썩음병원균(Botryosphaeria dothidea)에 대한 생물적 제어 능력을 검정하고 균주 동정을 실시하였다. 참다래 과수원에서 분리한 총 250여종의 단일균주 중에서 참다래에서 발생하는 과숙척음병원균에 대하여 길항작용이 우수한 균주를 1차적으로 6종 선발하였고, 이 중에서 참다래 과숙썩음병원균에 대하여 길항 작용이 96.0% 정도로 우수한 Strain #120을 최종적으로 선발하였다. 길항균 #120의 포자배열은 rectiflexibles 하였고, 포자표면은 smooth형이었으며, 분리균 세포벽내 LL-type이 DAP를 갖는 wall chemotype I 이었다. 길항균 #120의 균주 형태학적 특성, 생리 생화학적 그리고 화학 분류학적 특성 등을 종합하여 볼 때 길항균 #120은 Streptomyces sp. #120으로 동정되었다.

조선은 文으로 빚고 禮로 다듬은 文治國家이자 禮治의 공동체였다. 朱子學을 尊信했던 양반 사대부들은 ‘文治’ 구현의 충실한 수행자를 자임했고, 국가의 기초 단위인 ‘家’의 실현 공간인 마을에 대한 애착은 강렬했다. 그들에게 마을은 住居와 生活을 넘어 學術·文化를 자급하는 知識共同體로 착상되었고, 그것의 보편적 구현을 朱子學의 朝鮮化로 받아들였다. 인문 景觀으로서의 마을은 形成되는 것이 아니고 특정한 이념과 가치를 지닌 인간이 造成하는 공간이다. 그 공간의 우열은 그것을 디자인하는 인간의 능력과 직결된다. 시대를 내다보는 안목과 식견을 지닌 인간이 디자인에 개입할 때 그 공간은 形質의 수월성, 즉 格調를 담보할 수 있다. 안계마을 河氏의 本源的 思惟는 朱子學과 南冥學이었다. 전자가 보편성을 지닌 종적 思惟라면 후자는 특수성에 바탕한 횡적 가치였다. 이들은 양자를 종횡으로 엮에 자신들의 문화를 만들어 갔고, 또 그것을 통해 자신들의 존재성을 부각시키며 사회·학문적 영역을 확대해 나갔다. 15세기에 안계에 정착한 하씨들이 이 공간에 대한 우월적 지배력을 확보하고 주자학·남명학적 개념과 구도 속에서 마을을 경영하기 시작한 것은 17세기 초반이었고, 그 정점에는 그 시대의 嶺南學界가 선호했던 河弘度라는 학자가 존재하고 있었다. 그와 그 자손들이 조성하고 경영했던 文巖[門巖]·敬勝齋·慕寒齋·宗川書院·直方軒·光影亭·尼谷書堂·士山書堂 등의 학술문화 인프라는 안계마을의 학술문화적 지향과 정치사회적 노선을 반영하는 것이었다. 안계마을을 무대로 펼쳐졌던 그들의 삶의 모습은 300년의 시간 속에서 역사의 지층으로 남았고, 우리는 이 지층을 통해 그들의 삶을 반추하려 한다. 마을은 이런 것이다. 글은 인간의 필요와 이해에 따라 고칠 수도 있고, 곱게 단장할 수도 있지만 시간의 경과 속에 견고하게 다져진 삶의 자취는 섣부른 개변을 용인하지 않는다. 이것이 마을의 문화사가 지니는 質實하면서도 혹독한 장면이다.

This study was conducted to examine the effects of irrigation interval and fertilizer level on changes in soil chemical properties and growth of cut flower in soil retarding culture of standard chrysanthemum (Dendranthema grandiflorum) ‘Iwanohakusen'. The compound fertilizer (Poly-Feed, N-P-K 19-19-19) diluted with 1 g･L -1 were treated by irrigation intervals of 1 time/1 day (1.5 L･m -2 ), 1 time/2 days (1.5 L･m -2 ), 2 times/3 days (3 L･m -2 ) and 2 times/5 days (3 L･m -2 ). As irrigation interval was long, the nutrient contents of soil decreased. In 1 time/1 day treatment, NO3-N, K, and P2O5 contents of soil decreased, but Ca and Mg contents of soil did not change than before planting. The growth of cut flower, such as stem length, stem diameter, fresh weight, and weight of flower was the best in 1 time/1 day treatment, and was the worst in 2 times/5 days treatment. To examine the proper fertilizer level, the compound fertilizers (Poly-Feed, N-P-K 19-19-19) of 0.2, 0.4, 0.8, and 1.6 kg per 1.5t water were treated 1 time/1 day in 1,000 m 2 field. In fertilizer level of 0.8 or 1.6 kg, EC and nutrient contents of soil were higher or similar than before planting, and inorganic salts in soil were accumulating continuously. The growth of cut flower, such as stem length, number of leaves, weight, and diameter of flower bud was more effective in fertilizer level of 0.4 kg, but it was the worst in excessive fertilizer level of 1.6 kg. Therefore, fertigation of 0.4 kg compound fertilizer with 1 time/1 day in 1,000 m 2 field was the most effective for reasonable soil management and cut flower production of high quality in retarding culture of standard chrysanthemum ‘Iwanohakusen’.

In this paper, we describe the development of a bioreactor for a cell-culture experiment on the International Space Station (ISS). The bioreactor is an experimental device for culturing mouse muscle cells in a microgravity environment. The purpose of the experiment was to assess the impact of microgravity on the muscles to address the possibility of longterm human residence in space. After investigation of previously developed bioreactors, and analysis of the requirements for microgravity cell culture experiments, a bioreactor design is herein proposed that is able to automatically culture 32 samples simultaneously. This reactor design is capable of automatic control of temperature, humidity, and culture-medium injection rate; and satisfies the interface requirements of the ISS. Since bioreactors are vulnerable to cell contamination, the medium-circulation modules were designed to be a completely replaceable, in order to reuse the bioreactor after each experiment. The bioreactor control system is designed to circulate culture media to 32 culture chambers at a maximum speed of 1 ml/min, to maintain the temperature of the reactor at 36±1°C, and to keep the relative humidity of the reactor above 70%. Because bubbles in the culture media negatively affect cell culture, a de-bubbler unit was provided to eliminate such bubbles. A working model of the reactor was built according to the new design, to verify its performance, and was used to perform a cell culture experiment that confirmed the feasibility of this device.

Bacterial cellulose (BC) has played important role as new functional material for food industry and industrial products based on its unique properties. The interest in BC from static cultures has increased steadily in recent years because of its potential for use in medicine and cosmetics. In this study, we investigated culture condition for BC production by Acetobacter sp. F15 in static culture. The strain F15, which was isolated from decayed fruit, was selected on the basis of BC thickness. The optimal medium compositions for BC production were glucose 7%, soytone 12%, K2HPO4 0.2%, NaH2PO4ㆍ2H2O 0.2%, lactic acid 0.05% and ethanol 0.3%, respectively. The strain F15 was able to produce BC at 26℃-36℃ with a maximum at 32 ℃. BC production occurred at pH 4.5-8 with a maximum at pH 6.5. Under these conditions, a maximum BC thickness of 12.15 mm was achieved after 9 days of cultivation; this value was about 2.3-fold higher than the thickness in basic medium. Scanning electron micrographs showed that BC from the optimal medium was more compact than plant cellulose and was reticulated structure consisting of ultrafine cellulose fibrils. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose.

In general, since partial pollen is derived to sporophyte, anther culture efficiency is low, and practical application that is introduced in actuality breeding technology is not many. This study was carried out to improve regeneration of green plants through some culture environments contol in anther culture of naked brley. Among the factors related with plant regeneration, medium was effective in component containing L-glutamine 256 mg/L, L-proline 250 mg/L, IAA 1 mg/L and BAP 2 mg/L controlled in addition sucrose 30 g/L or maltose and sucrose mixed each 30 g/L to increase both plant regeneration and green plant regeneration rate. Also, adequate content of CuSO4 was the best at 1.25 mg/L (fifty-fold), it was tendency to decrease albino production rate. Starvation was effective at 30℃, 7 days in case of Saessalbori for plant regeneration and Dooweonchapssalbori at 30℃, 10 days with increasing green plant regeneration against albino. After plant regeneration, under acclimation by hydroponics, roots and shoots were well developed at 20℃, light control as 2,000～5,000 Lux and photoperiodic reaction by 14/10 as dark/light in the early growth stage. In acclimation method, plants acclimated in the modified Yoshida solution filled-vermiculite in GP pot is superior (100%), in which was controlled by temperature 20℃, pH 6.0 and relative humid 90% over, especially, after transplanting in pot growth of root, sheath and leaf are more active in 20℃ and 5,000 Lux control. For Vernalization, plants derived from anther culture of F1 naked barley was different from their parents with normal heading plant even about 50% in the F1 hybrids whose vernalization was strong, whereas the rest of plants derived from anther culture formed rosette, showing that normal growth were impossible.

To obtain the safety evaluation of the ginseng, residues of heavy metals in culture environment of ginseng on Punggi and Sangju, Kyeongbuk are surveyed. The concentration for component of ginseng on Punggi and Sangju were 14.12 mg/kg and 15.74 mg/kg, respectively. The concentration for general component such as crude fiber, ash, crude lipid, crude protein, carbohydrate, of ginseng were coincided between Punggi and Sangju. The concentration for As, Pb, Cd, and Hg in soil on Punggi were 14.24 ppb, 43.13 ppb, 8.73 ppb and 0.82 ppb, respectively. The concentration for As, Pb, Cd, and Hg in soil on Sangju were 19.20 ppb, 54.82 ppb, 15.90 ppb and 1.04, respectively. Residual heavy metals are not polluted in the soil with culture ginseng on Punggi and Sangju. The concentration for As, Pb, Cd, and Hg with ginseng on Punggi were 29.30ppb, 21.78 ppb, 1.32 ppb and 2.72 ppb, respectively. The concentration for As, Pb, Cd, and Hg with ginseng on Sangju were 3.22 ppb, 24.43 ppb, 1.44 ppb and 4.74 ppb, respectively. Also the detection concentration for As, Pb, Cd, and Hg in ginseng were also lower than the Korea Food & Drug Administration advisory level for heavy metal in herbal medicines. Residual heavy metals are not polluted in the ginseng on Punggi and Sangju at Kyeongbuk, Korea.

A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH4NO3, 0.3% KH2PO4, 0.3% K2HPO4, 0.02% MgSO4ㆍ7H2O, 0.0025% CaCl2ㆍ6H2O, 0.0015% FeSO4ㆍ7H2O in 1ℓ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 ㎖ of the same medium in 250 ㎖ flask. They were cultivated at 30 ℃ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.