시차주사 열분석기를 이용하여 poly(ethylene oxide)(PEO)/poty(styrene-co-acrylic acid) (SAA) blonds에 대한 등온 결정화 속도를 blend의 조성, 사용한 SAA의 공중합조성 및 결정화 온도에 따라 조사하였다. 실험결과는 Avrami 방정식을 이용하여 분석하였다. Avrami지수는 결정화 온도에 관계없이 거의 모든 blend 시료에서 2의 값을 나타내었다. 결정화 속도는 blend시료의 SAA 함량 및 사용한 SAA 공중합체의 아크릴산함량이 증가함에 따라 급격히 느려졌다.

Inhibitory effect of colchicine on growth and gravitropic responses in Arabidopsis root was explored to find whether there was an involvement of ethylene production. It has been known that cytoskeleton components are implicated in sedimentation of statoliths to respond to gravitropism and growth. The root growth was inhibited by 25% and 40% over control for 8 hr treatment of colchicine at a concentration of 10-5 M and 10-7 M, respectively. The roots treated with colchicine at the concentration of 10-7 M showed the same pattern as control in 3 hr, however, gravitropic response was decreased in the next 5 hr. The colchicine treatment at the concentration of 10-5 M inhibited the gravitropic response resulting in 60° of curvature. In order to better understand the role of colchicine, the production of ethylene was measured with and without the treatment of colchicine. Colchicine increased the ethylene production by 20% when compared to control via the activation of ACC oxidase and ACC synthase activity. These results suggest that the inhibition of the growth and gravitropic responses of Arabidopsis roots by the treatment of colchicine could be attributed to the rearrangement of microtubule, and increase of ethylene production.

This study was conducted to investigate the effect of 1-methylcyclopropene (1-MCP) and ethylene-absorbent on the qualities of Prunus mume fruit. Prunus mume fruits were stored without film packaging (Cont), packed in LDPE film (Cont-P), and packed with ethylene-absorbent (Cont-PE). Fruits were treated with 1-MCP (1 ppm) for 24 hr at 1℃. After treatment, fruits were packed in LDPE film (MCP-P) and with ethylene-absorbent (MCP-PE) and then stored at 1℃ for 8 weeks. Total soluble solids increased during storage but decreased after 6 weeks while total acidity decreased during storage. Cont was almost completely decayed after 8 weeks of storage while Cont-P, Cont-PE, MCP-PE, and MCP-P were 46, 69, 83, and 5% decayed, respectively. L value decreased but a value increased during storage in all samples. Firmness of peel and flesh of samples decreased gradually for 8 weeks. Respiration rate did not show any significant difference among samples. Ethylene production of Cont showed 0.05 μL/kg/h but immediately after 1-MCP treatment, it showed 0.02 μL/kg/h. Oxalic and malic acids decreased while citric acid increased during storage; fructose and glucose substantially decreased after 8 weeks whereas sorbitol and sucrose increased upto 4 weeks and then decreased thereafter. Based on these results, packing the fruits treated with 1-MCP could extend the freshness of Prunus mume fruit.

본 연구는 에테폰과 부형제를 혼합하여 에틸렌 발생 타 블렛을 제조하였고, 에틸렌 가스 활성조건을 구명한 결과 는 다음과 같다. 온도조건별 타블렛의 에틸렌 발생량은 온 도가 높을수록 많은 발생을 보였다. Activator pH 조건별 에틸렌 발생량은 pH 7.0 및 9.0 조건에서는 발생량이 약 2 ppm으로 미미하였으나, pH 13.0에서 타블렛의 에틸렌 발생량은 Prosolv 부형제 94.05 ppm, HPMC 부형제 126.28 ppm, Crosscamellose 부형제 100.11 ppm으로 각각 나타났 다. 제조된 타블렛의 마손도는 Prosolv 부형제 0.1%, HPMC 부형제 0.3%, Crosscamellose 부형제 54.1%로 나타나 Crosscamellose 타블렛은 부적합하다고 판단되었다. 붕해 도의 경우 각각의 부형제에 따라 큰 차이를 보였으며, Prosolv 부형제 1분, HPMC 부형제 7분 이상, Crosscamellose 부형제 5분으로 각각 나타났다. 최종 선정된 부형제인 Prosolv 타블렛의 시간대별 에틸렌 발생 측정결과 가스포집 20시간 까지 에틸렌 발생량은 꾸준히 증가하였지만 20시 이후의 발생은 미미하였고 지속적인 가스 발생은 처리 후 20시간으로 나타났다.

Regulation of fruit ripening may help extend fruit shelf life and prevent losses due to spoilage. Here, we investigated whether sound treatment could delay tomato fruit ripening. We treated harvested tomato fruits with low-frequency sound waves (1 kHz) for 6 h, and then monitored various characteristics of the fruits over 14-day period at 23±1°C. Seven days after the treatment, 85% of the treated fruits were green, versus fewer than 50% of the non-treated fruits. Most of the tomato fruits had switched to the red ripening stage by 14 days after treatment. Ethylene production and respiration rate were lower in the treated than non-treated tomatoes. Furthermore, changes in surface color and flesh firmness were delayed in the treated fruits. To investigate how sound wave treatment affects fruit ripening, we analyzed the expression of ethylene-related genes by quantitative real-time RT-PCR analysis. We found that the expression level of several ethylene biosynthetic and ethylene signaling pathway-related genes was influenced by sound wave treatment. These results demonstrate that sound wave treatment delays tomato fruit ripening by altering the expression of important genes in the ethylene biosynthesis and ethylene signaling pathways.

Regulation of fruit ripening may help extend fruit shelf life and prevent losses due to spoilage. Here, we investigated whether sound treatment could delay tomato fruit ripening. We treated harvested tomato fruits with low-frequency sound waves (1 kHz) for 6 h, and then monitored various characteristics of the fruits over 14-day period at 23±1°C. Seven days after the treatment, 85% of the treated fruits were green, versus fewer than 50% of the non-treated fruits. Most of the tomato fruits had switched to the red ripening stage by 14 days after treatment. Ethylene production and respiration rate were lower in the treated than non-treated tomatoes. Furthermore, changes in surface color and flesh firmness were delayed in the treated fruits. To investigate how sound wave treatment affects fruit ripening, we analyzed the expression of ethylene-related genes by quantitative real-time RT-PCR analysis. We found that the expression level of several ethylene biosynthetic and ethylene signaling pathway-related genes was influenced by sound wave treatment. These results demonstrate that sound wave treatment delays tomato fruit ripening by altering the expression of important genes in the ethylene biosynthesis and ethylene signaling pathways.

Currently, a technique to subdivide the process refining is introduced, can be divided into the steel slag. In this study, Free-CaO in the electric arc furnace slag by the ethylene glycol method for the quantitative analysis for the expansion to proceed with construction materials.

식물체에 대한 에틸렌의 다양한 반응에도 불구하고, 감자 가공 산업을 중심으로 에틸렌의 맹아억제제로서의 가능성에 관해서만 주로 검토되어 왔다. 그러나 본 연구에서는 씨감자에 에틸렌을 처 리하여 생리적 서령 조절제로서의 가능성을 확인코자 하였다. 국내 에서 가장 많이 재배되고 있는 ‘수미’ 감자를 대상으로 처리방법, 처리농도 등을 달리하여 저장 중 에틸렌 가스를 처리 한 후 휴면타파 및 맹아의 생장에 미치는 영향을 조사하였다. 에틸렌이 처리된 감자 괴경은 처리 후 1~2일에 급격히 호흡율이 상승해 감자 괴경의 에틸렌에 대한 민감한 반응을 확인할 수 있었다. 3일간의 단기간 에 틸렌 처리는 감자 괴경의 휴면을 약간 타파시키는 결과를 보였으 나, 2 μL/L 농도의 연속 처리구에서는 오히려 저장 40일까지 전혀 맹아가 되지 않아 뚜렷한 맹아억제 효과를 보였다. 에텔린 농도와 처리기간을 달리한 연속처리 시험에서는 2 또는 4μL/L 농도 처리 구 공히 뚜렷한 맹아억제 효과를 다시 확인할 수 있었고, 짧고 많은 수의 맹아가 발생되는 현상을 발견할 수 있었다. 이렇게 에틸렌이 처리된 씨감자를 온실 내 포트에 파종하여 재배한 결과 출현속도에 서는 큰 차이가 없었으나 에틸렌 처리에 의해 식물체당 줄기수와 복지수가 증가되었다. 수량성에서는 뚜렷한 경향 없이 처리 간 약 간의 차이를 보였으나 식물체당 괴경수는 에틸렌 처리에 의해 현저 히 증가되는 결과를 확인할 수 있었다. 이상의 결과를 종합해 볼 때, 저장중 씨감자를 대상으로 한 에틸렌 가스 처리, 특히 2~4μL/L 농 도범위에서 씨감자의 생리적 서령을 조절하는 유용한 수단으로 활 용될 수 있을 것으로 기대된다.

Blast furnace slag is recycled as high value-added material, while steel slag is difficult to recycle or is recycled as low value-added one relatively due to its expansion collapsability. It's property is caused by the high content of Free-CaO and unstable steel oxides. Currently, a technique to subdivide the process refining is introduced, can be divided into the electric furnace steel slag and reducing steel slag. In this study, Free-CaO in the electric arc furnace slag by the ethylene glycol method for the quantitative evaluation for the expansion to proceed with construction materials, electric arc furnace slag oxidation is to use.

This research evaluated the environmental impact of waste film used in the farming industry which is subject to voluntary agreement by Waste Charge System using Life Cycle Assessment (LCA). When analyzing the environmental scores applying weighted values, production of raw materials, production of goods and disposal had nearly 73%, 15% and 11% of contribution to the environmental impact and also, amongst impact categories, resource depletion and global warming had 63% and 32% of contribution respectively. For resource depletion which had the highest contribution, production of raw materials, production of goods and disposal occupied around 86%, 9% and 4% of it, and the impact category of resource depletion that belongs to production of raw materials, accounted for about 54% of the whole environmental score. Here, over 95% of it was caused by crude oil. When investigating the contribution of each phase to global warming that is the key issue of low carbon green growth, production of raw materials, production of goods and disposal had respectively, 45%, 28% and 27% of contribution. As the result, production of goods and disposal had higher contributions than resource depletion. The entire contribution of production of goods and disposal to global warming was about 17%, and it is predicted that it would bring a huge impact, considering the possibility of establishing improvement plans. In the production of goods, electricity consumed was the main cause of global warming, and electricity used in the recycling process and incineration occupied approximately 52% and 42% of the disposal stage. In conclusion, we should pay attention to energy used in the production of goods and recycling for improving an environmental competitiveness of goods, and reduction of energy would be able to satisfy economic feasibility and environmental efficiency of the whole process of agricultural PE film.

UV light irradiation is known to give beneficial effects on fresh produce preservation. A container system equipped with UV-LED was fabricated for storing cherry tomatoes under computer-controlled conditions of intermittent on-off cycles (1 hour on/1 hour off). Wavelength (365 and 405 nm) and physical location of the LED (2 and 5 cm above fruit) were studied as variables affecting the respiration, ethylene production and quality preservation of the fruits at 10 and 20℃. 365 nm wavelength gave much higher radiation intensity than 405 nm, and intensity on surface decreased in inverse proportion to square of distance from LED. When compared to non-irradiated control, UV-LED irradiation decreased the respiration by 5-10% at 10℃ while there was no obvious effect at 20℃. Ethylene production was reduced when the fruits were placed at 5 cm distance, while there was no significant difference from control at 2 cm location. The reduction of ethylene production at 5 cm was more pronounced at 20℃. UV-LED irradiation was shown to have delayed increase or lower concentration in carotenoids compared to control treatment. Any negative effect of UV-LED irradiation on ascorbic acid content and firmness was not observed.

The ripening behavior of three apple cultivars, ‘Tsugaru’, ‘Hongro’ and ‘Fuji’ was distinctive and the involvement of POLYGALACTURONASE(PG) in the fruit softening process was confirmed to be ethylene dependent. Fruit softening is genetically coordinated by the action of several cell wall enzymes, including PG which depolymerizes cell wall pectin. Also, loss of firmness is associated with increasing of the ripening hormone, ethylene. In this work, climacteric ripening of three apple cultivars, Tsugaru, Hongro and Fuji, producing different ethylene levels and ripening responses, was examined. Correspondingly in Fuji, a linear and basal ethylene level was observed over the entire period of measurements, and Tsugaru and Hongro displayed a typical climacteric rise in ethylene production. Transcript accumulation of genes involved in ethylene biosynthesis (MdACS3 and MdACO1) and MdPG1 was studied in Tsugaru, Hongro and Fuji cultivars. Expression of MdACO1 transcripts was shown in all three ripened apple fruits. However, the MdACS3 and MdPG1 were transcribed differently in these cultivars. Comparing the MdPG1 of ‘Tsugaru’, ‘Hongro’ and ‘Fuji’, structural difference was discovered by genomic Southern analysis. Overall results pointed out that MdACS3 and MdPG1 play an important role in regulation of fruit ripening in apple cultivar.

배의 저장성 향상 방법 구명 측면에서 수확한 화산 배에 16시간 동안 1-MCP 처리(0, 0.5, 1.5 ppm) 후 상온() 저장기간에 따른 품질 특성을 조사하였다. 저장전과 저장 8주 후의 과피 색도는 Hunter L, a 및 b값 모두 1-MCP 1.5 ppm 처리구에서 변화가 가장 적었다. 관능평가 값은 1-MCP 0 ppm 처리구는 4주째까지, 0.5 ppm 처리구는 6주째 까지, 1.5 ppm 처리구는 7주째까지 좋다 이상의 수준으로

We carried out the effect of Si levels (0.5, 1.0 and 2.0 mM) on ethylene and salicylic acid (SA) of hydroponically grown rice plants, after 6h, 12h and 24h of Si application. All samples were analyzed after applying wound stress for 30 minutes. In 6 h treatments, 0.5 and 1.0 mM of Si decreased ethylene production by 7% and 9.9% respectively, while elevated level of Si (2 mM) insignificantly affected ethylene production under same conditions. However 0.5 and 1 mM of Si enhanced endogenous SA contents by 2.6% and 17.3% respectively, while 2 mM of Si decreased SA contents by 3% as compared to control. Samples analyzed after 12h of 0.5, 1 mM and 2 mM Si application showed reduction of 11%, 4% and 1.7% respectively, while SA contents increased by 4% and 9.5% in 0.5 and 1 mM Si treatments. Samples analyzed after 24 h of Si treatment showed that 0.5 and 1.0 mM of Si inhibited ethylene production by 12.6% and 18.2% respectively, while 2 mM Si increased ethylene production by 11.9% as compared to control. It was observed that the SA content of rice was almost similar to control in 0.5 mM Si applied treatments, while 1 mM and 2 mM of Si increased SA contents by 6.5% and 6.9% as compared to control. It was concluded that Si alleviates the effect of wounding stress in rice by regulating ethylene and SA production.

The study was carried out to establish an improved protocol for shoot organogenesis and plant regeneration from leaf explant cultures of Scrophularia buergeriana M. with the treatment of ethylene inhibitors [silver nitrate (AgNO3), aminoethox-yvinylglycine (AVG), Cobalt chloride (CoCl2)]. The regenerated shoots obtained from leaf explant cultures on MS medium containing 2 mg/l BAP, The additions of AgNO3. AVG and CoC12 substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L, 7 mg/L, and 3 mg/L respectively, The regenerated shoots could be easily rooted with 0.1 mg/L IBA treatment. The noted plants were hardened and transferred to vermiculite with a 85% survival rate where they grew normally.