As the consumption of corn increases, the production of by-products is also increasing. In this study, functional substances and antioxidant activities of corn by-products were investigated to determine their applicability as functional materials. Total polyphenol, flavonoid contents, and ABTS antioxidant activity were the highest in leaves. The total phenolic acid content was the highest in cobs; p-coumaric and ferulic acid were the main components. Phytosterol content in leaves and stems was high at 64 and 50 days after sowing, 261.4 and 274.7 mg/100 g, respectively. β-sitosterol of the leaves and stems was the highest, 149.2 and 138.7 mg/100 g. The total phytosterol content of grains and by-products harvested 106 days after sowing was in the order of: corn silk, husk, grain, leaf, stem, and cob. Among the phytosterols, β-sitosterol was the highest, followed by stigmasterol and campesterol, and the composition was different depending on part. The total phytosterol content was the highest in husk and corn silk, but the stems and leaves, which account for 50% of the total corn biomass, also contained high phytosterol content. Therefore, corn leaves and stems are expected to be used as functional raw materials for the development of functional materials in the future.

본 연구에서는 시엽의 기능성 화장품 소재로서의 활용 가능성을 평가하기 위해 항산화와 인 간섬유아세포인 CCD-986sk 세포에서의 항주름 효과를 알아보았다. 전자공여능 측정, ABTS+ radical 소거능 측정을 통해 항산화 활성을 확인한 결과, 시엽은 1,000 μg/ml 농도에서 대조군인 ascorbic acid 와 농도 의존적으로 유사한 항산화 활성을 보였다. Elastase 저해 활성 측정, Collagenase 저해 활성 측 정을 통해 항주름 효과를 확인한 결과, 시엽은 1,000 μg/ml 농도에서 대조군인 epigallocatechin gallate 와 농도 의존적으로 유사한 항주름 효과를 나타내었다. UVB로 유도된 CCD-986sk 세포 내 pro-collagen type I의 합성률과 MMP-1의 저해률을 측정한 결과 대조군인 EGCG는 20 μg/ml에서 90.2%, 시엽은 30 μg/ml에서 88.5%의 pro-collagen 합성률을 보였다. 또한 EGCG 20 μg/ml와 시엽 30 μg/ml에서 각각 33.0%, 40.8%의 MMP-1 저해율을 확인하였다. Western blot을 통하여 시엽의 pro-collagen type I과 MMP-1의 단백질 발현량을 측정한 결과 UVB 단독군 대비 시엽을 함께 처리했 을 때 pro-collagen type I의 단백질 발현량이 증가하고 MMP-1의 단백질 발현량이 감소하는 것을 확 인하였다. 위 실험결과에 따라 시엽이 UVB 자극에 의한 광노화를 예방하고 항산화, 항주름 효과가 있음 을 확인하여 화장품의 천연물 소재로서의 이용이 기대된다.

To enhance the efficacy of Abeliophyllum distichum leaves, extracts were prepared using different solvents for hydrolytic enzyme-treated Abeliophyllum distichum leaves. Physicochemical quality and antioxidant activity were measured. Soluble solids, reducing sugar, ascorbic acid, flavonoids, and polyphenols contents showed the lowest values in the control without enzyme treatment. However, they showed high contents in ethanol extract. In the case of enzyme treatment, their values were higher than those of the control. In particular, verbascoside content increased about 220 times more than that of the control group when treated with enzymes and extracted with 50% ethanol. pH was lowered upon enzymatic treatment. Regarding DPPH radical scavenging activity, for enzyme-free, 25% ethanol extract showed the highest activity among extracts with different solvents. For cellulase and pectinase-treated leaves, water extract showed the highest DPPH radical scavenging activity among extracts with different solvents. For leaves treated with enzyme combination, 50% ethanol extract showed the highest DPPH radical scavenging activity among extracts with different solvents. Regarding ABTS radical scavenging activity, it was generally higher in the 50% ethanol extract than in the water extract and 25% ethanol extract. In particular, verbascoside content was increased when the extract was prepared by co-treatment with enzymes and 50% ethanol.

This study compared the antioxidant and mineral properties of the leaves and seeds of fifteen Korean adzuki bean (Vigna angularis L.) breeding lines. This study was conducted in an attempt to expand the use of Korean adzuki bean leaves. The potassium, calcium, magnesium, and sodium contents of the leaves were significantly higher than the seeds, in particularly, the potassium content. The leaves had approximately 3.3 times higher potassium content than the seeds. For instance, the potassium content of YA1317 leaves was 21% higher than that of Arari. The total polyphenol content and ABTS activity of Adzuki bean leaves were significantly higher than the seeds, as opposed to the total flavonoid content and DPPH scavenging activity. Among the 15 breeding lines, YA1402 had 1.2~3.2 times higher antioxidant content and activity as compared to the Arari variety. It was concluded that adzuki bean leaves had higher mineral content, antioxidant component and activity as compared to the seeds. Therefore, adzuki bean leaves could be used an ingredient for dishes and as a medicine.

This study aimed to investigate the biological activities, including polyphenolic content, flavonoid content, and antioxidant activities, of various cultivars of Korean perilla leaves. The results indicated that among nine cultivars (Namcheon dlggae, Saedora, Nulbora, Donggel 1, Donggell 2, Soim, Sangyeop, Somirang, and Saebom) of perilla leaves, the total polyphenolic content (gallic acid equivalent mg/g, GAE) was the highest in “Nulbora,” while it was lowest in Namcheon dlggae. Moreover, flavonoid content in the extracts of nine cultivars leaves was in the range of 132.93~268.50 mg catechin equivalent/g sample. The antioxidant effects of the perilla leaves were determined using two different in vitro bioassays measuring DPPH and ABTS radical-scavenging activities. The results revealed that antioxidant activity was also higher in “Nulbora” compared with other cultivars. Xanthin-oxidase-inhibition activity ranged from 65.65% to 80.58%, with “Nulbora” exhibiting the highest activity, although the difference with other cultivars was not significant. “Nulbora” extracts reduced the expression of pro-inflammatory genes and several cytokines, including IL-6 activation induced by LPS in macrophages in the range of 100–50 μg/mL. These results suggest that extracts from perilla leaves can be used as bioactive and functional materials that could be important in industrial applications in the future.

본 연구는 RGB, 초분광 센서를 이용하여 시기별 사과 잎의 엽록소와 질소 함량을 예측하여 사과 나무 잎의 질소 영양을 진단하기 위해 수행되었다. 분광 데이터는 사과나무 ‘홍로 /M.9’ 2년생을 대상으로 고해상도 RGB와 초분광 센서로 촬 영 후 영상처리를 통해 취득하였다. 식물체 데이터는 촬영이 끝난 직후 엽록소와 잎 질소 함량을 측정하였다. 엽록소 측정 기의 SPAD meter, RGB 센서의 개별 파장, 컬러 식생지수 및 초분광 센서의 214개의 파장과 식물체 데이터를 이용하여 회 귀분석을 실시하였다. 엽록소와 잎 질소 함량 데이터는 시기 와 상관없이 질소 시비량에 따라 통계적으로 유의한 차이가 나타났다. 잎은 시기가 지나면서 잎에 있던 영양분이 과실로 전이되어 색이 옅어졌으며 RGB센서의 경우 Red파장에서 시 기와 상관없이 통계적으로 유의한 차이가 나타났다. 초분광 센서의 경우 두 시기 모두 질소 시비 수준에 따라 가시광 영역 보다 비가시광 영역에서 차이가 크게 나타났다. 반사값를 이 용하여 식물체 특성의 예측 모델 결과 엽록소, 잎 질소함량 모 두 초분광 데이터를 이용한 부분최소제곱 회귀분석을 이용하 였을 때 성능이 가장 높게 나타났다(chlorophyll: 81% / 63%, leaf nitrogen content: 81% / 67%). 이러한 원인은 RGB 센서 에 비해 초분광 센서는 좁은 FWHM과 400－1,000nm의 넓 은 파장 범위를 가지고 있어 질소 결핍에 의한 스트레스로 인 해 작물의 분광학적 해석이 가능했을 것으로 판단된다. 추후 분광학적 특성을 이용하여 전 생육 시기의 수체 생리, 생태 모 델 개발 및 검증 그리고 병해충 진단 등 연구를 통해 고품질, 안 정적인 과실 생산 기술 개발에 기여될 것으로 사료된다.

An increasing trend embracing the 2030 generation mindset through the new word ‘newtro’, which means reinterpreting the past and selling it in the present, has been gaining traction. The 2030 generation who seek new experiences and desserts is growing. The unique dessert market is expected to continue to grow in the future. Thus, this study devised a pine needle madeleine. Madeleines were made by varying the proportions of pine needle powder added to 0%, 1%, 3%, 5%, and 7%, and specific gravity, moisture, color, physical properties, and antioxidant properties were measured. The L-value decreased significantly (p<0.001) as the amount of pine needle powder added increased, and the a-value increased (p<0.001). The total polyphenol content, DPPH, and ABTS gradually increased significantly (p<0.001). Through this study, it was possible to confirm the quality and characteristics of madeleines using pine needle powder, which has excellent antioxidant properties, and it will become basic data for the development of various desserts using pine needle powder.

This work reports utilization of apple leaves as a source of activated carbon. Activated carbon from apple leaves is prepared by two different methods, thermal activation where AC1 is obtained and chemical activation using H3PO4 and ZnCl2 where AC2 and AC3 are obtained, respectively. XRD analysis revealed that all types of prepared ACs have a semi-crystalline nature with a mean crystallite size of 13, 21.02, and 39.47 nm for AC1, AC2, and AC3, respectively. To identify the most suitable desorption temperature, the exothermic behavior was discovered for the three types of ACs by DSC. The exothermic onset temperatures are 340 °C, 200 °C, 400 °C, or AC1, AC2, and AC3, respectively. The point of zero charge for the three types of ACs is 8.6, 7.3, and 2.5 for AC1, AC2, and AC3, respectively. The BET surface area analysis data demonstrated that mesoporous structure was developed in AC1 and AC2, while a microporous structure was developed in AC3. Quantum chemical calculations for ACs is carried out using Density Functional Theory (DFT). Application of the prepared ACs in adsorption of basic dye C.I. base blue 47 is studied. The maximum removal efficiency was 65.1%, 96% and 99% for AC1, AC2, and AC3, respectively under the influence of different operating aspects. Adsorption data are modeled by Langmuir, Freundlich, and Temkin isotherms. The data revealed that adsorption of basic dye C.I. base blue 47 on AC1 follows Langmuir isotherm and adsorption on AC2 and AC3 follows Freundlich isotherm.

The purpose of this study was to investigate the volatile flavor compounds of Ligularia stenocephala and Ligularia fischeri, edible wild plants. The volatile flavor compounds were isolated by the simultaneous distillation extraction method and analyzed by GC-MSD (gas chromatography-mass selective detector). Forty-eight volatile flavor compounds were identified in the extracts from L. stenocephala and the major compounds were sabinene, cis-ocimene, trans-caryophyllene, and β-elemene. Fifty-one volatile flavor compounds were identified in the extracts from L. fischeri and the major compounds were α-pinene, germacrene-D, transcaryophyllene, endo-1-bourbonanol and 1-limonene. The common volatile flavor compounds between two plants were transcaryophyllene, sabinene, β-elemene and β-cubebene etc. However, α-phellendrene and myrcene were identified in L. stenocephala, but were not identified in L. fischeri. However α-pinene, germacrene-D and limonene were identified in L. fischeri but were not identified in L. stenocephala.

The purpose of this study was to remove lead and arsenic ions from aqueous solutions using the activated carbon prepared from Citrus limon tree leaves. Characteristics of the prepared adsorbent were studied thoroughly using BET, SEM, EDS and mapping, XRD, and RAMAN analyses. The results of experiments showed that the highest adsorption efficiencies were 97.67% and 95.89% for Pb (II) and As (III), respectively. Additionally, the adsorbent was successfully regenerated four times and therefore it was able to perform the adsorption and desorption processes well. Moreover, the results of adsorption kinetics showed that the pseudo second-order kinetic model was more effective for the description of adsorption mechanism of both metals. Furthermore, the equilibrium studies indicated that Langmuir and Freundlich isotherm models were desirable for lead and arsenic ions, respectively.

W. B. 예이츠의 시는 1920년대 이후 중국에 소개되었는데, 그는 아일랜 드의 대표적 시인이다. 그의 시는 중국의 시에 큰 영향을 끼치는데 특히 나인 리브스파 시인들 중 무 단과 연 게이자에 대한 영향이 크다. 비교연구를 통해서, 본 논문은 무 단은 자신의 시 스타일 형성에 예이츠에게 영향을 받았으며, 연 게이자는 자신의 시이론을 형성하는데 많은 자양분을 흡수했다는 사실을 증명한다.

This study investigated the volatile flavor composition of essential oils extracted from Synurus deltoides (Arr.) Nakai. The essential oils extracted from the aerial parts of plants by the hydrodistillation extraction method were analyzed by gas chromatography (GC) and GC-mass spectrometry. Ninety-six (98.76%) volatile flavor compounds were identified in the essential oil extracted from fresh leaves of S. deltoides (Arr.) Nakai. The major compounds were 6,10,14-trimethyl-2-pentadecanone (19.91%) and phytol (12.38%). Ninety-seven (97.81%) volatile flavor compounds were identified in the essential oil extracted from shade-dried leaves of S. deltoides (Arr.) Nakai. The major compounds were phytol (51.71%), di(6-methylhept-2-yl) phthalate (7.66%), and 6,10,14-trimethyl-2- pentadecanone (4.23%). Quantitative variations of 6,10,14-trimethyl-2-pentadecanone, phytol and di(6-methylhept-2-yl) phthalate according to different state of S. deltoides (Arr.) Nakai can serve as a quality index of essential oils used in the food industry.

점박이응애는 광식성 초식자로서, 사과의 주요 해충 중 하나이다. 사과 품종에 따라 점박이응애의 밀도 형성이나 피해 규모가 다르게 나타난다. 세 가지 품종, 쓰가루, 홍로, 후지의 잎에 대한 점박이응애의 이동 선호성, 발육속도, 그리고 산란능력이 다른지를 시험하였다. 잎 절편을 이용한 실험 결과, 이동 선호성은 쓰가루나 홍로보다 후지 잎에서 높았다. 알에서 성충까지 발육기간은 후지 잎에서 가장 짧았고 홍로에서 가장 길었다. 암컷 성충 수명은 후지에서 가장 짧았다. 암컷의 총산란수는 사과 품종에 따라 다르지 않았지만, 일 평균 산란수는 후지에서 높았다. 생명표 분석 결과 홍로 잎에서 점박이응애의 적합도가 가장 낮게 나타났다. 이상의 결과는 사과원에서 품종에 따른 점박이응애 발생 양상의 차이를 설명 해주고 있으며, 향후 다양한 사과 품종에 대한 평가를 통해, 품종별 응애류 관리 전략 개발에 기초 자료가 될 것이다.

In this research, Pb (II), Co (II), and Ni (II) toxic heavy metal ions adsorption from synthetic aqueous system have been studied using the activated carbon prepared from Citrus limetta leaves. Therefore, the relationship between the adsorption parameters (solution pH, dosage of adsorbent, temperature, initial concentration of the ions, and adsorption time) and the removal percentage of the prepared adsorbent have been investigated. Additionally, the adsorbent was analyzed through BET, SEM, EDX, FTIR, and XRD analyses. According to the results, the maximal adsorption efficiencies for heavy metal ions were achieved in pH = 6, the adsorbent dosage of 1 g/L, temperature = 25 °C, the ion initial concentration of 5 mg/L, and contact time of 60 min, which were 99.53%, 98.63%, and 97.54% for Pb, Co, and Ni ions, respectively. Based on Kinetic studies, the performance of pseudo-second-order kinetic model was better than pseudo-first-order model for the description of time-dependent behavior of the process. Additionally, the equilibrium data were fitted by Langmuir and Freundlich isotherms, while the former performed better than the latter. The maximum adsorption capacity values for Pb, Co, and Ni ions were achieved equal to 69.82, 60.60, 58.139 mg/g, respectively. Considering the thermodynamic data, the studied processes were exothermic and spontaneous.

This study examined the bioactive compound content and the antioxidant activities of bitter melon (Momordica charantia Linn.) leaves. The content of vitamin C, beta-carotene, and total carotenoids was 69.77, 45.68, and 65.08 mg/100 g, respectively. To investigate the antioxidant capacity, bitter melon leaves were extracted using various concentrations of ethanol (60, 80, or 100%). Highest content of total polyphenols (18.07 mg gallic acid equivalent/g) and flavonoids (4.53 mg cathechin equivalent/g) was found in the 100% ethanolic extract of the leaves (E100). Also, the E100 extract showed the highest levels of 2,2'azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities. Reducing power was also the highest (39.21 mg Trolox equivalent/g) in E100 extract. The E100 extract effectively inhibited lipid peroxidation by 91.45% compared to the control group. Also, the E100 extract showed a cytoprotective effect against oxidative stress in HepG2 cells and decreased the generation of intracellular reactive oxygen species. These results suggest that bitter melon leaves could be regarded as a potential source of natural antioxidants.