포도 '캠벨얼리' 품종의 결과지당 적정 엽수를 구명하기 위해 만개 30일 후에 결과지당 2송이를 착과시키고, 첫 번째 송이는 70~75립, 두 번째 송이는 35~40립으로 조절하고 변색기 이후 과실의 품질변화를 조사하였다. 과실의 당도는 변색기 이후 28일 동안 급격히 증가하였으나, 그 이후에는 일정한 수준을 유지하였다. 결과지당 15엽 처리구는 착색초기부터 빠르게 증가되어 착색 28일 후에 16.0˚Bx 이상 되었으나, 결과 지당 10엽 처리구는 12.0˚Bx로 증가폭이 적었고, 결과 지당 5엽 및 0엽 처리구는 당도 축적이 거의 없었다. 산도는 모든 처리구에서 28일 동안 감소되었고, 결과 지당 엽수가 많을수록 감소폭도 컸다. 결과지당 15엽 처리구는 변색기 이후 7일 동안 빠르게 감소된 후 수확기까지 꾸준히 감소되어 0.5%로 되었으나, 결과지당 5엽 및 0엽 처리구는 감소세가 완만하여 수확기에도 산 함량이 1.0%로 높았다. 과피색은 결과지당 15엽에서 hunter L과 b값이 변색기 이후 7일 동안 빠르게 감소되었고, 그 이후에는 완만하게 감소되어 수확기에 가장 낮은 값을 나타냈고, hunter a값은 변색기 이후 7일 동안 빠르게 증가되었고, 그 이후에는 약간 감소되었다. 그러나 결과지당 5엽 및 0엽 처리구는 hunter L과 b값이 변색기부터 완만하게 감소되었고, hunter a 값은 착색초기에는 현저히 낮았으나, 수확기까지 꾸준히 증가되었다. 총 안토시아닌은 결과지당 15엽 처리구에서 변색기 7일 후부터 빠르게 증가된 후 변색기 28일 후부터는 일정한 수준을 유지하였고, 다른 처리구는 변색기 이후 큰 차이가 없었다.

This study was conducted to investigate the effects of donor cell treatments on the production of transgenic cloned piglets. Ear fibroblast cell obtained from NIH MHC Inbred minipig was used as control. The GalT knock-out/CD46 knock-in (GalT/CD46) transgenic cell lines were established and used as donor cells. The reconstructed GalT/CD46 embryos were surgically transferred into oviduct of naturally cycling surrogate sows (Landrace × Yorkshire) on the second day of standing estrus. Unlike control (1.2 kV/cm,, 75.4%), the fusion rate of the GalT/CD46 donor cells was significantly higher in 1.5 kV/cm, (84.5%) than that of 1.25 kV/cm, (20.2%) (p<0.01). When the number of the transferred embryos were more than 129, the pregnancy and delivery rates were increased to 13/20 (65%) and 5/20 (25%) compared to less than 100 group [1/6 (16.7%) and 0/6 (0%)], respectively. To analyze the effect of donor cell culture condition on pregnancy and delivery rates, the GalT/CD46 donor cells were cultured with DMEM or serum reduced medium. In serum reduced medium group, the pregnancy and delivery rates were improved to 8/12 (66.7%) and 5/12 (41.7%) compared to DMEM group [3/7 (42.9%) and 0/7 (0%)], respectively. In conclusion, it can be postulated that an appropriate fusion condition and culture system is essential factors to increase the efficiency of the production of transgenic cloned piglets.

경남농업기술원 화훼육종연구소에서 1997년부터 교배 조합 육성을 위하여 국내의 재배농가와 종묘업체로부터 유전자원을 수집한 후, 2000년부터 2006년에 걸쳐 교배 및 계통선발육종을 하였다. 2006년부터 2008년까지 3회 의 특성검정을 거쳐서 호접란 신품종 ‘옐로우 크림’을 개발하였다. ‘옐로우 크림’은 황색스트라이프 대륜계 꽃 을 가진 품종으로 설판은 적색을 띤다. 꽃 크기가 10.3 cm 이상이 될 정도로 꽃이 큰 편이며 대비품종에 비해 화형은 둥글고 립이 붉으며 꽃잎의 모양이 반원형 으로 아름다운 모양을 가지고 있다. 또한 꽃배열도 우수 하며 꽃수도 많은 편이다. 엽색은 연한 녹색이며 생육속 도도 빠르고 재배하기가 용이하다. 평균 꽃 수명 또한 55일 이상으로 길다. 소수의 품종만이 시장에 나와 있는 황색 스트라이프 계통의 신품종으로서 국내외 시장에서 기호도가 높을 것으로 판단된다.

Supply chain for the economic development of East Asian economic development model, the study's purpose is to establish. Korea, Japan, China, Russia and Asia, including North Korea and get the status of economic development by focusing on key issues and proposed solutions, within a few years of the coming of a new East Asian economic integration of new supply chain would like to prepare for the era. Weight The weight of the center to the center of East Asia, looking for techniques to approach the supply chain scenario, the economic development strategies and measures were studied. East Asia's economic potential value of the supply chain, identify and recognize the importance and benefits should be Presents detailed country-specific development strategies and leading Asian economies will have to pay the supply chain. Republic of Korea's leading East Asian economies in order to supply the industry, industry development strategy as detailed in the running to move. North and South Korea for economic integration, the era of supply-chain economy, you need to prepare calmly.

Flow control of flow field is essential to design efficient elements relating to fluid machineries. In this study, flow characteristics of rectangular prism with center gap through-flow at different aspect ratio was investigated to flow control. It was used a FLUENT 6.3 version to study flow field. It was found that the through-flow disturbs the development of vertical velocity component and decreased the vortex size and critical value of a rapid change in pressure coefficient distribution.

In this study, gradient porous Al-Cu sintered body was fabricated by powder metallurgy processing. Al-Cu powder mixtures were prepared by low energy ball milling with various milling time. After ball milling for 3h, the shape of powder mixtures changed to spherical type with size of 100~500 . Subsequently, Al-Cu powder mixtures were classified (under 150, 150~300 and over 300 ) and compacted (20, 50 and 100 MPa). Then, they were sintered at for various holding time (10, 30, 60 and 120 min) in atmosphere. The sintered bodies had 32~45% of porosity. As a result, the optimum holding time was determined to be 60 min at and sintered bodies with various porosity were obtained by controlling the compacting pressure.

본 연구는 외부 관광객이 많은 인천광역시 월미공원을 대상으로 지역주민과 외부 관광객 사이의 공원 운영과 관리에 대한 인식 차이를 조사 분석함으로써 공원 이용 활성화와 효과적인 운영관리 방안을 모색하기 위해 수행되었다. 자료 수집은 2009년 5월 1일부터 4일간 총 222명의 공원 방문객을 대상으로 현지설문조사를 통해 이루어졌다. 조사결과, 방문횟수 및 목적, 보완 시설물 등에서 지역주민과 외부 관광객 간 차이를 보였으며, 두 집단 모두 인근 관광지에 대한 인식이 공원 이용만족도에 영향을 미치는 것으로 인식함에 따라 공원 구역 내뿐만 아니라 주변지역의 운영 관리에도 만전을 기해야 할 것이다.

This report describes a case of odontogenic cyst with keratinization and dysplastic change of lining epithelium, which showed the manifestation of inflammatory radicular cyst, clinically. A 28-year-old man complained of dull pain in the right mandibular molar region. Radiographically a well-defined oval cystic lesion with non-vital teeth, a common finding in radicular cyst, was observed. Microscopically, the lining epithelium of the cyst demonstrated both keratinization and severe epithelial dysplasia. Atypical findings such as hyperchromatic nuclei, increase of N/C ratio and drop shaped rege ridge were observed in the lining epithelium. However, definite invasion into fibrous connective tissue was not found. Immunohistochemically, the dysplastic lining epithelium was highly positive for proliferative marker, Ki-67. Based on the dysplastic changes of lining epithelium, this periapical lesion would be considered to be signs of malignant change. From this case, we conclude that definitive diagnosis by microscopical examination should be made, even if the periapical lesion would be clinically considered as inflammatory radicular cyst.

Human pituitary tumor transforming gene 1 (PTTG1), which is a newly identified proto-oncogene, is highly expressed in normal pituitary tissues containing proliferating cells and in several cancers. Also, PTTG1 has been known as a securin to involve in the regulation of c ell-cycle and in t he p rogression o f tumor. B u t the effect o f PTTG1 in o ral squamous cell carcinoma (oral SCC) h as not b een studied yet. The objective of this study is to analyze the expression of PTTG1 in oral SCC cell lines (YD-10B and YD-15) and to evaluate the effect of PTTG1 on oral SCC cell lines for the migration effect by PTTG1 siRNA treatment. Western blot, migration assay, and zymography were performed to evaluate the effects of PTTG1 on the expression of MMP-2/-9 and migration activity after PTTG1 siRNA treatment. PTTG1 was expressed in oral SCC cells lines, otherwise, significantly decreased after PTTG1 siRNA treatment. There is no difference in expression of MMP-2 regardless of PTTG1 siRNA treatment. However, the enzyme activity of MMP-9 was significantly decreased. In addition, the migration activities of oral SCC cells were significantly decreased after PTTG1 siRNA treatment (p<0.001). These results suggested that the down-regulated PTTG1 could inhibit the migration of human oral SCC cells through the low MMP-9 expressions. Therefore, these findings provide a useful guideline for the migration mechanism of oral SCC depend on PTTG1 expression.

목적: RGP 렌즈의 형상에 따른 파면수차를 분석하였다. 방법: 본 연구의 취지에 동의하고 안질환이 없고 굴절교정수술을 시행하지 않은 대학생 38명(남 21명, 여 17명, 24.71±0.76세)을 대상으로 나안시력(0.36±0.34), 교정시력(1.06±0.13), 각막이심률(0.51±0.11) 과 RGP 렌즈 착용 전 후 안구수차를 측정하였다. 결과: 고위수차에서 코마수차는 유의한 변화가 없었으며, 세조각수차와 구면수차는 4가지 디자인 모두 유의하게 감소하였다(p< 0.05). 동공크기와 고위수차의 상관성은 A 렌즈에서 코마수차(r=0.406, p=0.000) 및 세조각수차(r=0.232, p=0.044)와 유의성이 있었다. 각막이심률과 고위수차의 상관성은 없었다. 등가구 면굴절력과 고위수차의 상관성은 C 렌즈에서만 코마수차와 유의성이 있었다(r=0.324, p=0.004). 각막굴절 력과 고위수차의 상관성은 코마수차는 B 렌즈(r=0.310, p=0.006)에서, 세조각수차는 D 렌즈(r=0.255, p =0.026)에서 유의성이 있었다. 구면수차는 A 렌즈(r=0.358, p=0.002), B 렌즈(r=0. 501, p=0.000) 및 C 렌즈 (r=0.479, p=0.000)에서 상관성이 있었다. 결론: 4가지 RGP 렌즈에서 가장 변이가 큰 수차는 구면수차이며, 렌즈의 전면에 경계가 없고 일정한 면을 가지고 있는 비구면 디자인에서 고위수차가 효과적으로 감소하는 것으로 나타났다. 구면수차를 줄이는 렌즈의 디자인이 개발된다면 시력의 질을 개선시킬 수 있을 것으로 사료된다.

Melamine has raised international concerns for its catastrophic health effects from tainted infant formula. This report concerns the developmental validation of a sensitive HPLC/MS/MS and GC/MS methods about melamine and cyanuric acid in human urine and serum. Analytical detection ranges of LC/MS was from 0.2 to 5.0 ng/mL and 2.0 to 60.0 ng/mL about melamine and cyanuric acid, respectively. The limits of quantification and confirmation are 0.2 ng/mL for both analytes in human urine and serum by LC/MS/MS. The range of recovery was 91.6%, and 107.6% for cyanuric acid and melamine in urine, respectively. The range of precision coefficient variation was from 2.0%, to 11.8% for cyanuric acid and melamine in urine. The range of recovery was from 94.9%, to 119.0% about cyanuric acid and melamine in serum, respectively. The range of precision coefficient variation from was 3.7%, and 13.5% about cyanuric acid and melamine in serum. Analytical detection ranges of GC/MS were 5.0 to 100.0 ng/mL about melamine and cyanuric acid, respectively. The limits of quantification and confirmation are 5.0 ng/mL for both analytes in human urine and serum by GC/MS. The range of recovery was from 83.7%, to 114.5% for cyanuric acid and melamine in urine, respectively. The range of precision coefficient variation was 3.5%, and 10.7% for cyanuric acid and melamine in urine. The range of recovery was 94.4%, and 110.7% for cyanuric acid and melamine in serum,respectively. The range of precision coefficient variation from was 3.9%, and 13.8% for cyanuric acid and melamine in serum. Several changes were taken to optimize performance by this method.

Understanding the metapopulation structure and movement of a species are required for conserving the species. In this paper, migration patterns and connectivity of patches of a threatened butterfly, Pamassius bremeri Bremer, were postulated using the mark-release-recapture (MRR) technique in a habitat located in the mid-southern region of the Korean peninsula. A total of 194 individuals were captured (137 males and 57 females) and, of them, 93 individuals (73 males and 20 females) were recaptured during the MRR experiment. The migration analysis showed 23-150% immigration and 28-53% emigration. There were high correlations between the migrating individuals and the distance between patches, but there was no correlation between migrating individuals and patch size or between migrating individuals and the number of host plants. Consequently, the migration of butterflies occurred frequently between closer patches, while patch size and quantity of the food plant had minor effects on migration behavior. Additionally, males migrated more frequently than females. Analysis of the migration patterns of P. bremeri showed that the central patch played an important role on linking patch groups and more frequent migrations were monitored between nearby patches than between the remote patches. This study suggested that active migrations take place between the neighboring multiple patches and these are accelerated if there is a stepping-stone patch between them.

In general, a valve body of the automatic transmission(AT) is controlled by the clutch, the brake and lubricating oil flow in a hydraulic system and lubricant flow for each valve can be adjusted independently. To increase the lifetime of AT, the lubrication flow rate in a valve body for a 6 speed AT based parallel hybrid electric vehicle must be provided with proper oil distribution and control. In this study, we carried out several experiments without the inner parts of AT and with a AT assembly. The variation of the flow rate on oil temperature and pressure between an oil supply port and the outlets of the lubrication port was evaluated and analyzed. In the case of AT without the inner parts, it was evident that as the oil required for an operation of the clutch and brake was discharged from the outlet port, the flow rate from each lubrication port is decreased. However, the flow rate of the AT assembly was slightly increased. In addition, the lubrication flow rate was increased with increasing the oil temperature, and also it was reduced with increasing the oil pressure. Details of the resulting data are discussed.

목적 : 본 연구는 자유로운 탐색으로서의 다감각 환경에서 치매노인들에게 제시된 스노즐렌 도구들에 대한 감각별 선호도를 조사하고자 하였다. 연구방법 : 본 연구의 대상자는 강원도 W시에 위치한 노인전문요양원에서 치매 진단을 받은 노인 17명(남 3명, 여 14명)이며, 연구진행 방법은 스노즐렌 도구 사용에 익숙한 작업치료사 1인이 시각적 자극(6가지), 청각적 자극(1 가지), 촉각적 자극(6가지), 후각적 자극(1가지)이 주어지는 총 14가지 스노즐렌 도구를 일정한 순서로 1주일 간 격으로 1명당 총 2 회기(40분/1회기) 동안 탐색하도록 제시하였다. 각 도구별 선호도 측정을 위하여 2명의 작업치 료사는 7점 리커트 척도를 사용하였다. 측정자간 점수 일치도는 평균 82.5%(1 회기: 80.6%, 2 회기: 84.4%) 이었 다. 결과 : 연구 결과 둥근돌로 만든 촉각판, 곡식상자, 물기둥, 프로젝터, 밍크털 등의 익숙한 촉각 자극에 대한 선호도 는 높은 반면, 싸이키 조명, 아로마 향, 거친 촉각판, 광섬유와 같이 익숙하지 않고 자극적인 도구들에는 부정적인 반응을 보였다. 또한 클래식의 이완음악, 진동물침대, 밀러볼 등에는 반응을 보이지 않았다. 결과를 종합적으로 보 았을 때 치매 노인들은 후각, 청각적 자극보다 촉각 및 시각자극에 긍정적인 반응을 보였다. 결론 : 본 연구결과를 토대로 스노즐렌 치료실을 구성 및 배치시 우선순위에 촉각, 시각적인 자극을 필수적으로 고 려하고 추후 환자별로 선호하는 도구에 따른 감각과 도구를 배치한다면 치매환자에게 보다 효과적인 프로그램을 운영하는데 도움이 될 것이다.