This study was carried out to investigate the effects of semen on reproductive ability in crossbred Korean native chicken (KNC, 58-wk old). The body weight, volume of semen and concentration of spermatozoa, were 2.96 g, 0.40 ml, 36.58×108/ml, respectively, in KNC. The fertility and hatchability were 94.8% and 78.8% respectively in crossbred KNC. KNC(Y) was high compared to other strains in fertility. The other strains were not significantly different among 6 strains. The results of this experiment indicated that hatchability of (G) was high compared to other strains. The result of this study could be available to genetic improvement of reproductive traits as a basic reference in KNC strains. To achieve the more effective improvement of reproductive traits, addition research such as genetic parameter evaluation should be performed.

본 연구의 목적은 20대 표준체형 여성을 대상으로 하여, 소재에 따른 플레어 스커트의 실제착의와 가상착의에 따른 시각적 이미지를 비교 분석하고, 시각적 이미지와 역학적 특성간의 상관관계를 분석하는 데에 있다. 본 연구는 드레이프 특성이 확연히 다른 5종류의 소재를 사용하였다. 실험에 사용되어진 플레어 스커트의 실제착의와 가상착의의 이미지는 사진으로 제공되었으며, 피설문자는 20대의 의류학 전공의 여성이었다. 자료의 분석은 SPSS Ver.12.0 프로그램을 사용하여 통계 처리하였으며, 연구 문제별로 요인분석, 일원변량분석(One way ANOVA), T 검정(t-test), 던컨테스트(Duncan test)를 실시하였다. 시각적 이미지에 대한 요인분석 결과 '드레이프성', '매력성', '신체 보정성', '부피감', '활동성' 의 총 5 가지 요인이 분석되었다. 시각적 이미지중 '부피감'의 경우 G, 무게, 두께와 같은 역학적 특성들과 밀접한 상관관계를 나타냈으며, 3차원 의복 시뮬레이션과 실제착의간의 시각적 이미지는 소재에 따라 유의한 차이점을 나타냈는데, 실크나 폴리에스터 소재와 면, 린넨, 양모소재간 이미지 차이는 소재의 무게와 두께에 따라 영향을 많이 받는 것으로 나타났다.

The aim of this study was to evaluate the effect of α-tocopherol on blastocysts development and subsequent cryosurvival of the vitrification. The α-tocopherol(0, 100, 200, 400 μM) was added in to culture medium for the bovine embryos. The blasocysts from the α-tocopherol and untreated control groups were then frozen-thawed, and their cryosurvival was assessed by in vitro culture for 48 h. There were no differences in the overall cleavage rate(56.14±4.66, 58.18±4.70, 62.97±6.86 and 51.17±7.28) among four treatment groups. However, in blastocyst development and total cell number were significantly higher in α-tocopherol 200 μM(38.60±7.12; 106.33±3.50) to culture medium than other treatment groups(29.30±5.24, 31.60±7.12 and 26.37±4.18; 101.36±5.12, 97.27±2.87, and 91.23±7.52 respectively). Before and after vitrification, the total cell number and blastocyst development of embryo were significantly higher in July to August than September to October. In conclusion, addition of α-tocopherol 200 μM to in vitro bovine embryo culture medium was beneficial for improving embryo quality by decreasing the embryo damage blsstocysts cell number and improving the tolerance of the embryos to cryopreservation.

In the present study, we identified differentially methylated region (DMR) upstream of Dnmt1o and Dnmt1s gene in early porcine embryos. Porcine Dnmt1o had at least one DMR which was located between —530 bp to —30 bp upstream from transcription start site of the Dnmt1o gene. DNA methylation analyses of Dnmt1o revealed the DMR to be hypomethylated in oocytes, whereas it was highly methylated in sperm. Moreover, the DMR upstream of Dnmt1o was gradually hypermethylated from oocytes to two cells and dramatically changed in the methylation pattern from four cells to BL stages in an in vivo. In an IVF, the methylation status in the DMR upstream of Dnmt1o was hypermethylated from one cell to eight cells, but demethylated at the Morula and BL stages, indicating that the DNA methylation pattern in the Dnmt1o upstream ultimately changed from stage to stage before the implantation. Next, to elucidate whether DNA methylation status of Dnmt1s upstream is stage-by-stage changed in during porcine early development, we analyzed the dynamics of the DNA methylation status of the Dnmt1s locus in germ cell, or one cell to BL cells. The Dnmt1s upstream was highly methylated in one and eight cells, while less methylated in two, four, morula, and BL cells. Taken together, our data demonstrated that DNA methylation and demethylation events in upstream of Dnmt1o/Dnmt1s during early porcine embryos dramatically occurred, and this change may contribute to the maintenance of genomewide DNA methylation in early embryonic development.

본 연구는 유기재배 벼 시비에 있어서 주로 이용되는 유기자재가 토양화학성과 벼의 수확량과 품질에 어떠한 영향을 미치는 지를 구명하기 위해서 수행되었다. 전남지역 벼 유기재배에서 양분공급원으로 많이 이용되는 금수강산골드를 compost I으로 하였다. 식물성 성분이 많이 함유된 자재(쌀겨팰렛®)를 compost II, 생선가공부산물을 별도로 추가 제조한 자재(참달콤®)를 compost III, 그리고 동물성과 식물성이 혼합된 자재(한가위®)를 compost IV로 나누어서, 벼 이앙 후에 표층시비로 토양에 골고루 처리하였다. 유기자재내의 탄소 :질소율이 상대적으로 낮았던 compost I과 III가 compost II와 IV에 비교하여 시기별로 유기자재내 질소의 무기태화가 빠르게 진행되었다. 유기자재에 따른 토양의 화학성은 별다른 영향을 받지 않았고 토양의 시기별 유기물 함량은 유기자재 투입량이 많았던 compost II와 IV가 높게 나타났고 이는 biomass C에도 영향을 주었다. 처리에 따른 벼 수확량은 시용 첫해에는 compost I에서 높았지만 시용 2년차와 3년차에는 처리에 따른 차이는 나타나지 않았다.

The overexpression of Phosphoprotein Enriched in Astrocytes (PEA15) gene is commonly found in human diabetic patients. The overexpression of this gene in skeletal muscle and fat tissues have been reported to cause insulin resistance, thereby impairing insulin stimulated glucose uptake. We introduced a gene of mouse PEA15 (mPEA15) and enhanced green fluorescent protein (EGFP) into fertilized one cell pig zygotes using microinjection, and produced a piglet that showed overexpression of mPEA15 in the muscle tissues and expression of EGFP in the ear tissues and hooves. RT-PCR RFLP, southern blot and FISH analysis showed that the tissues carried the transgene. Real-time RT-PCR and western blots demonstrated that PEA15 gene was overexpressed in the various tissues and muscle tissues, respectively. These facts suggest that expression vector system is normally expressed in the trnasgenic (TG) pigs. To use as animal diseases model for type 2 diabetes, further study is necessary to confirm whether diabetes occur in these TG pigs, especially insulin resistance.

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high performance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recoveries of DON were 88.76 and 95.40% at the levels of 200 and 1,000 μg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 μg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 μg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 μg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 μg/kg for them, individually.

This study aims to provide preliminary review for standardization of methods for evaluating reduction performance of pollution-reducing building materials and to select commercially available pollution-reducing building materials for assessing their current status. The ISO 16000-23 and -24 standards were used as references for standardizing the test methods. The sub test categories—stability of the supply air concentration, type of the supply air, and supply air concentration—which were not included in the standards were selected and conducted for the purpose of this study. Five (5) wall materials and 2 ceiling materials were tested for formaldehyde reduction performance and 3 wall materials were tested for toluene reduction performance. The study included experimental review of the essential test criteria such as stability of the supply air, internal stability of the chamber, and recovery rate. The samples tested exhibited some reduction performance for formaldehyde but little reduction performance for toluene.

포유동물 수정란의 동결보존기술은 최근 기후 변화에 따른 생물종 다양성을 보존하기 위해서 중요하게 여겨지는 연구 분야이다. 따라서 멸실 위험에 처한 동물의 개량과 증식, 보존과 복원 및 생명공학의 분야에 이르기까지 응용 기술은 다양하게 이용되어진다. 본 연구에서는 한우 수정란의 동결 후 생존성 향상을 위해서 동결 방법에 따른 체내 외수정한의 내동성을 조사하였다. 완만동결에 따른 체내 외수정란의 동결 융해 후 수정란의 재확장률은 89.6%와 81.5% 그리

비정형 초고층건물을 위한 구조시스템 중에서 다이어그리드 구조시스템은 구조적인 효율성 및 조형성 때문에 널리 사용되고 있다. 근래에는 초고층건물을 위한 구조시스템으로 메가부재의 조합을 통하여 횡방향 강성을 효과적으로 발휘할 수 있는 메가프레임 시스템이 널리 사용되고 있다. 두 가지 구조시스템의 장점을 혼합한 다이어그리드 메가프레임 구조시스템은 미래형 초고층건물에 적용될 유망한 구조시스템으로 평가받고 있다. 그러나 이러한 다이어그리드 메가프레임 구조시스템을 적용한 건물의 거동을 예측하기 위해서는 매우 많은 수의 절점과 요소로 이루어진 유한요소 모델을 해석해야 하므로 상당한 양의 해석시간과 엔지니어의 노력이 필요하게 된다. 따라서 본 연구에서는 다이어그리드 메가프레임시스템을 적용한 초고층건물의 거동을 효율적으로 해석할 수 있는 기법을 제안하여 다이어그리드 메가프레임 초고층건물의 해석과 설계에 소요되는 시간과 노력을 줄이고자 한다. 이를 위하여 다이어그리드 메가프레임의 특정을 활용한 효율적인 모형화기법과 행렬응축기법을 사용하여 해석에 사용되는 자유도수를 최소화한 해석기법을 제안하였다. 예제구조물의 해석을 수행하여 본 연구에서 제안된 해석방법과 일반적인 해석방법에 의한 결과와 비교함으로써 제안된 방법의 효율성과 정확성을 검증하였다.

To address global climate change, various governments are investing in electric vehicle research and, especially in Korea, the application of electric vehicles to public transportation. The lithium batteries used in electric vehicles typically have an exp

The purpose of this study was to assess the agreement of manual muscle testing (MMT) and test-retest reliability of a hand held dynamometer for the posterior gluteus medius muscle, with and without lumbar stabilization, using a pressure biofeedback unit for patients with low back pain. The pressure biofeedback unit was used to minimize the substitute motion of the lumbopelvic region during hip abduction in patients lying on their side. Fifteen patients with low back pain participated in this study. A tester determined the MMT grades of the posterior gluteus medius with and without the pressure biofeedback unit. Active hip abduction range of motion with an inclinometer and the strength of their posterior gluteus medius using a hand held dynamometer were measured with and without the pressure biofeedback unit in the MMT position. The agreement of the grade of muscle strength in the MMT, and intra-rater reliability of both the active hip abduction range of motion and the strength of posterior gluteus medius were analyzed using the weighted kappa and intraclass correlation coefficient (ICC), respectively. The agreement of MMT with the pressure biofeedback unit (weighted kappa=.92) was higher than the MMT (weighted kappa=.34)(p<.05). The inclinometer with pressure biofeedback unit measurement of the active hip abduction range of motion had an excellent intra-rater reliability (ICC=.90). Also, the hand held dynamometer with pressure biofeedback unit measure of strength of the posterior gluteus medius had a good intra-rater reliability (ICC=.85). Therefore, the test for muscle strength with pressure biofeedback unit will be a reliable method for the determination of the MMT grades or amount of posterior gluteus medius muscle strength and the measurement of the range of motion for hip abduction in patients with low back pain.

Although there have been various studies related to the body's movement from a sitting to a standing position (sit-to-stand task), there is limited information on the kinematic changes on the frontal and transverse planes. The purpose of this study was to ascertain how pelvic tilt affects kinematic changes in the frontal and transverse planes in the hip and knee joints during a sit-to-stand task. For this study, 33 healthy participants (13 female) were recruited. Each participant rose from a sitting to a standing posture at his or her preferred speed for each of three different pelvic tilt trials (anterior, posterior, and neutral), and the measured angles were analyzed using a 3-D motion analysis system. A one-way repeated measure analysis of variance was performed with Bonferroni's post hoc test. In addition, an independent t-test was carried out to determine the sex differences in hip and knee joint kinematic changes during the sit-to-stand tasks. The results were as follows: 1) The hip and knee joint angle in the frontal and transverse planes showed a significant difference between the different pelvic tilt postures during sitting in the pre-buttock lift-off phase (pre-LO) (p<.05). Compared to the posterior pelvic tilt posture, the anterior pelvic tilt posture involved significantly greater hip joint adduction and internal rotation, knee joint adduction, and reduced internal rotation of the knee joint. 2) Sex differences were found with significant differences for males in the initial and maximal angles in the frontal plane of the hip and knee joint (p<.05). Females had a significantly smaller initial abduction angle of the hip joint and a significantly greater maximal angle of the hip adduction joint. These results suggest that selecting a sit-to-stand exercise for pelvic tilt posture should be considered to control abnormal movement in the lower extremities.

본 연구에서는 주간동안 온실 내에서 발생되는 잉여 태양에너지를 분석하고, 또한 잉여 태양에너지의 적정 축열 시스템 설계에 필요한 기초자료를 제공할 목적으로 수행하였다. 분석에 이용된 기상자료는 표준기상년 데이터로서 이용하여 국내 주요 지역을 대상으로 온실 형태별로 잉여 태양에너지를 분석하였을 뿐만 아니라 소요 난방에너지 등도 분석 및 검토하였다. 이상의 결과를 요약하면 다음과 같다. 9개 지역을 대상으로 지역별 잉여 태양에너지를 대해 분석한 결과, 난방에너지 대비 잉여 태양에너지 비율은 온실 형태별로 각각 약 212.0~228.0%로서 제주가 가장 높게 나타났다. 그 다음으로 부산, 광주, 진주, 대구, 대전, 전주, 수원, 및 대관령 순으로 나타났다. 그리고 온실 형태에 관계없이 몇 몇 지역을 제외 하면 잉여 태양에너지만으로 소요 난방에너지를 거의 대체할 수 있을 것으로 판단되었다.

A survey of consumer awareness and attitudes was conducted about genetically modified (GM) foods and the labeling regulations. The questionnaires were distributed to 4,620 consumers who lived in a variety of areas in Korea, and 4,076 people responded. The consumers were asked about knowledge, labeling information, and the source of obtaining information about GM foods. More than 11.5% of the consumers had never heard about GM foods and 86.9% of consumers had less than a normal level of knowledge about GM foods. No statistically significant relationship was found between genders, but the teachers group had moderate knowledge (p<0.001). In total, 28.4% of consumers did not know the GMO labeling regulations. They answered that the reason to buy GM food was do not know>nothing wrong>create benefit>think as safe>inexpensive. The answers to the question of what was the first benefit were: solve food shortage>functional and nutritious food>cultivate in bad condition>nothing>various cultivars. They answered that the worst factor was the next generation effect>environmental disruption. Regarding the development of GM food in Korea, males answered do not know>stronglyrecommend>defer>strongly suppress. Female answered: don't know>defer>strongly recommend>strongly suppress. More than half of the respondents did not have much information about GM foods; 88.3% of respondents answered they did not have educational experience about GM food.

Optimization study of the hot water extraction for enhancing antioxidative activity from Auricularia auricula was performed by Taguchi approach using orthogonal matrix L9(34) method. The correlation between DPPH radical scavenging activity and the components of samples extracted from different extraction conditions were also analyzed. The correlation coefficient between DPPH radical scavenging activity and melanin content of A. auricula was 0.93, indicating 'good correlation'. The optimum extraction conditions were obtained at the extraction time of 1 hr. temperature of 85oC, solid: water ratio of 1: 40(w/v) and frequency of 2 times. Under these conditions, values of maximum DPPH free radical scavenging activity and melanin contents of A. auricula were 67.21±2.17 and 52.94±2.10 mg/g, respectively. Melanin content of 1.6 times and DPPH free radical scavenging effect of 130% were enhanced by optimization.

Physiological characteristics of brown rice and black rice were investigated to provide the fundamental information of physiological property of rice and to show the potential of rice as a functional ingredient. Bioactive compounds were extracted from brown and black rices with aqueous solvents like 80% ethanol. Total phenolics, flavonoids and antioxidative capacity of brown and black rices’ extracts were determined. Brown rice showed much higher amount of total phenolics and flavonoid contents as well as antioxidative capacity than those of milled rice indicating that most of bioactive compounds are located in the bran layer. Black rice showed higher total phenolics and flavonoid contents and antioxidative capacity than those of brown rices. The highest antioxidative capacity was obtained from Heugjinju followed by Heugseol, Sinnongheug-chal, Hopum and Samkwang. This result indicated that antioxidative capacity is affected by total phenolics and flavonoid contents. Both brown and black rices contained higher amount of ferulic acid than that of p-coumaric acid.

The purpose of this paper is to research Gunnar Asplund's Woodland Chapel (1918-20) in terms of the revernacularization of classicism and to investigate into the matter of the constructional logic. The term 'revernacularization of classicism' was used by Alan Colquhoun to explain the process to return to the pure sources of classical architecture, and the case of a successful fusion of classicism and vernacular traditions was suggested by Demetri Porphyrios through Scandinavian Doricist sensibility in the early 20th century. Porphyrios's classicism, not as style but as sensibility, is premised on a constructional logic of vernacular, and is to achieve an aesthetic quality by its mythical elaboration. Woodland Chapel, a representative of the Scandinavian Doricism according to him, illustrates characteristics of the revernacularized classicism as in the fact that it thickly displays vernacular images at the same time as relying on classicism; in the return to primitive simplicity; and in the mythopoeic power. However, the constructional logic of this building was obscured in the capital of the portico columns, the interior dome, and the whole structure of the roof. Confronting this paradox, we have to remember that although Porphyrios emphasized the constructional logic he opened an aesthetic exit of the mythical elaboration, which is in accord with the concept of the tectonic as the poetics of construction. Woodland Chapel assumes atectonic features but is never anti-tectonic. Asplund intensified a poetic effect by setting the myth over construction in the chapel, and so it can be seen as a key example of the revernacularized classicism with the Doricist sensibility.