A novel L. harbinensis VF was isolated from fermented vinegar and identified through biochemical characteristics and 16S rRNA sequencing analysis. Characteristics of probiotics were studied for acid and bile salt tolerances, hemolytic activity, antibiotic resistance, antimicrobial activity, cell surface hydrophobicity, and aggregation. The survival rates of the isolate were maintained at 68.9% and 95.6% after 3 h incubation at pH 2.0 and 2.5 and were over 74% at bile salt concentrations of 0.3% and 0.5%. The hemolytic activity was confirmed to be -hemolysis. The isolate showed broad antibiotics-resistance in over 12 antibiotics except for trimethoprim/sulfamethoxazole, compared to L. plantarum (KCTC 3108), used as control, and previously reported L. harbinensis. Antimicrobial activity was confirmed against pathogens, B. cereus, E. coli O157:H7, L. monocytogenes, and S. typhimurium, except S. aureus, and it was attributed to produced organic acids (low pH). The hydrophobicity for xylene and chloroform were 16.7% and 20.4%, respectively, lower than those of L. plantarum (41.2% & 49.9%). The auto-aggregation and coaggregation with pathogens were 83.8% and 49.2-87.6% at 24 h incubation, respectively, higher than those of L. plantarum. From this study, L. harbinensis VF is likely to be used as a probiotic strain and is a strong candidate for commercial application.

This study selected the optimal conditions for enzyme treatment that can promote the extraction of functional components from Salvia plebeia (SP) and confirmed solubilization characteristics, functional component contents, and applicability evaluation in pilot-scale, antioxidative, and anti-inflammatory activity. The optimal conditions of pectinase, 1%, and 2 h (P) were determined through the enzyme treatment of SP. The water-soluble index and total polyphenol content were the highest in P compared to 100oC reflux condition for 2 h (CON). Component analysis performed using liquid chromatography-mass spectrometry revealed that flavonoid contents were increased 6.6-9.5 times in the P compared to CON. There was no difference in component content when comparing the measurement lab-scale and pilot scale. CON and P indicated cytoprotective effect against H2O2-induced RAW 264.7 cells. CON and P pre-treatment also reduced the production of nitric oxide and proinflammatory cytokines in LPS-induced RAW 264.7 cells. Furthermore, P showed higher antioxidant and anti-inflammation activities than CON. These results suggested that P has a higher concentration of bioactive compounds through enzyme treatment than that obtained from CON. Thus, it can be used as a primary material for industrial utilization, such as developing functional food materials using SP.

오페라 작곡가 베르디의 『레퀴엠』은 대규모 관현악의 다양한 악기들과 8성부에 이르는 극적 인 종교적 합창곡이며 동시에 음악회를 위한 예술적 가치를 높이 평가 받고있는 작품이다. 다양 한 극적인 요소로써 금관악기를 비롯한 장엄한 음향과 가사처리, 모방적이고 반복적인 작곡기법 등이 있다. 본 논문은 종교적 합창곡에서 찾아볼 수 있는 대중적인 요소들을 베르디의 종교적 진혼곡『레퀴엠』을 통해 살펴보고자 한다. 우선 합창에서의 대중성은 가사를 편집하고 합창에 있어서 극적인 성부의 효과적으로 배치하고 있다. 가사 처리에 대한 작곡가의 의도는 장례 미사에 쓰여 지는 기능성을 가진 음악이라기보다는 예술적인 차원을 강조하여 대중성을 높인다고 할 수 있다. 두 번째, 연주회장에서 금관악기 등의 공간적 배치는 청중에게 현장감과 몰입감을 높인다. 소리의 공간감 과 입체감은 음악회에 대한 보다 만족스러운 결과를 가져다주며 실제 연주회장에서 작곡가에 의해 창출 된 음향적 의도가 효과적으로 실현될 수 있다. 마지막으로 합창음악에서의 대중성을 가지기 위해서는 선율이 단순하고 명확하며 합창 성부들을 수직적으로 화음의 구성 및 배치하며 또한 극단적인 셈여림 등으로 대중성을 높이고 있다.

3D printing is widely used in product development and prototype manufacturing, and is expected to become universal across various industries with the development of 3D printing-related technologies. However, parts made by Fused Deposition Modeling(FDM) 3D printing using the commonly used stacking manufacturing process, show low tensile strength and hardness. The decreased mechanical properties of these parts limit their use as structural elements. In this study, we aim to investigate the relationship between ultrasonic treatment of PLA parts produced by FDM 3D printing and their mechanical properties. Specifically, we analyze the effects of ultrasonic annealing on the mechanical properties of PLA parts using the tensile test specimen.

Thermoelectric materials and devices are energy-harvesting devices that can effectively recycle waste heat into electricity. Thermoelectric power generation is widely used in factories, engines, and even in human bodies as they continuously generate heat. However, thermoelectric elements exhibit poor performance and low energy efficiency; research is being conducted to find new materials or improve the thermoelectric performance of existing materials, that is, by ensuring a high figure-of-merit (zT) value. For increasing zT, higher σ (electrical conductivity) and S (Seebeck coefficient) and a lower к (thermal conductivity) are required. Here, interface engineering by atomic layer deposition (ALD) is used to increase zT of n-type BiTeSe (BTS) thermoelectric powders. ALD of the BTS powders is performed in a rotary-type ALD reactor, and 40 to 100 ALD cycles of ZnO thin films are conducted at 100oC. The physical and chemical properties and thermoelectric performance of the ALD-coated BTS powders and pellets are characterized. It is revealed that electrical conductivity and thermal conductivity are decoupled, and thus, zT of ALD-coated BTS pellets is increased by more than 60% compared to that of the uncoated BTS pellets. This result can be utilized in a novel method for improving the thermoelectric efficiency in materials processing.

최근 낮은 경사도와 편리한 보행 환경 조성으로 많은 사람들이 쉽게 접할 수 있는 무장애숲길 이 서울 도심 내에 조성되면서 이용자가 늘고 있다. 그러나 무장애숲길은 비슷한 용어인 무장 애 나눔길, 무장애 산책로, 자락길 등 여러 명칭과 혼용하여 쓰이고 있고 무장애숲길의 이용자 의 만족도와 관련된 연구 또한 미흡한 실정이다. 이에 본 연구에서는 기존 선행연구들을 토대 로 도심에 조성된 다양한 형태의 길을 비롯한 무장애숲길에 대한 개념을 정립하였다. 또한, 가 장 최근에 조성되어 인프라 조성 및 향후 체계적 관리방안 마련의 필요성이 있는 대모산 자락 길(무장애 나눔길)을 대상으로 방문한 이용객들의 중요도 및 만족도 인식을 파악하였다. 연구 결과를 요약하면 첫째, 무장애숲길은 경사가 비교적 완만한 산의 초입 산자락에 평균 8% 이내 의 데크 산책로를 조성해 휠체어를 탄 장애인도 쉽게 산을 오를 수 있게 조성된 곳으로 정의 할 수 있다. 둘째, 대모산 자락길 이용자의 중요도 및 만족도에 영향을 주는 요소는 적은 교통량 및 주차시설, 교통 시설과 쾌적성 요인, 휴게시설, 근처 통신시설 순으로 나타났다. 셋째, 대모 산 자락길에 대한 중요요인의 긍정 및 부정적 요소들을 비교한 결과, 접근성, 관리가 잘 된 보 행자 통로, 안전 및 보안, 정보 센터의 가용성 등 18가지 긍정적인 요소가 있음을 알 수 있었다. 이와 같이 본 연구에서는 기존 선행연구들을 토대로 도심에 조성된 다양한 형태의 길을 비롯한 무장애숲길에 대한 용어를 정리하고 대모산 자락길의 이용자중요도 및 만족도를 분석하였다. 연구의 결과는 이용자 만족을 고려한 무장애숲길 조성계획 및 관리에 기초자료로 사용될 수 있 을 것으로 기대된다.

Following the social requirement to strengthen field supervision of the asbestos containing materials (ACM) abatement process with regard to asbestos school buildings, this study was conducted to understand the status and characteristics of airborne asbestos that may potentially occur after the ACM abatement process is completed. In the area where a series of asbestos abatement processes were finally completed, comprehensive area air sampling was performed. For sample analysis, Transmission Electron Microscopy (TEM) was used according to The Asbestos Hazard Emergency Response Act (AHERA) method and Phase Contrast Microscopy (PCM) analysis was also performed. Airborne asbestos was detected in 29.5% of the total samples, and the average concentration was 0.0039 ± 0.0123 s/cc (12.3 ± 38.9 s/mm2). 4.5% of the total samples exceeded the AHERA standard (70.0 s/mm2) and the average concentration was 0.0528 ± 0.0256 s/cc (167.2 ± 82.0 s/mm2). Airborne asbestos was no longer detected at the point when AHERA is exceeded after re-cleaning. Most of the detected asbestos was chrysotile (94.4%) and the structure types of asbestos were Matrix (41.4%), Fiber (39.9%), Bundle (10.8%), and Cluster (7.8%). Among the asbestos structures detected through transmission electron microscope analysis, the asbestos structures satisfying PCM-equivalent structures were found to be 6% of the detected asbestos, indicating that there is a limitation of the PCM analysis to check the airborne asbestos in that area. As a result of reviewing the status of airborne asbestos that may potentially occur and the type and dimensions of asbestos structure detected in the area, since the airborne asbestos exposure caused by poor field supervision for the ACM abatement process could not be ruled out, thorough management is necessary. In addition, the result of this study could be used as scientific evidence for establishing and strengthening policies related to ACM abatement, including cases of school buildings.

The recent increase in earthquake activities has highlighted the importance of seismic performance evaluation for civil infrastructures. In particular, the container crane essential to maintaining the national logistics system with port operation requires an exact evaluation of its seismic response. Thus, this study aims to assess the seismic vulnerability of container cranes considering their seismic characteristics. The seismic response of the container crane should account for the structural members’ yielding and buckling, as well as the crane wheel’s uplifting derailment in operation. The crane’s yielding and buckling limit states were defined using the stress of crane members based on the load and displacement curve obtained from nonlinear static analysis. The derailment limit state was based on the height of the rail, and nonlinear dynamic analysis was performed to obtain the seismic fragility curves considering defined limit states and seismic characteristics. The yield and derailment probabilities of the crane in the near-fault ground motion were approximately 1.5 to 4.7 and 2.8 to 6.8 times higher, respectively, than those in the far-fault ground motion.

본 연구는 미용 전공 대학생의 비대면 수업 경험이 학습몰입에 미치는 영향에 대해 알아보고 With Corona 시대에 맞는 미용 교육산업에 기초자료를 제공하기 위함에 목적이 있다. 미용 전공 대학생 300명을 대상으로 2022년 06월 07일부터 06월 21일까지 자가기입식 설문법을 실시하였으며 총 286부를 표본으로 실증 분석하였다. SPSS ver. 21.0 프로그램을 활용하여 빈도분석, 요인분석, 탐색적 요인분석, 기 술통계분석, 상관관계분석, 다중회귀분석법을 사용하여 연구하였다. 그 결과, 비대면 학습경험을 요인 분석 한 결과 수업만족 2개의 하위요인으로 분석되었으며 학습몰입을 요인 분석한 결과 학습 즐거움과 학습몰입 의 2개의 하위요인으로 분석되었다. 수업 활동이 학습몰입의 하위요인인 학습 즐거움(β=.279, p<.007)과 학습몰입(β=.221, p<.031)에 통계적으로 유의미한 정(+)의 영향을 미치는 것으로 나타났다(p<.05). 수업 만족이 학습몰입의 하위요인인 학습몰입(β=.223, p<.041)에 통계적으로 유의미한 정(+)의 영향을 미치는 것으로 나타났다(p<.05). 본 연구결과를 토대로 비대면 수업 경험이 학습몰입에 영향을 미친다는 사실을 알 수 있었으며 이를 통해 시대에 맞는 효율적 비대면 교육 방안이 활발히 모색되기를 기대하는 바이다.

본 논문에서는 구조물의 부분 변위값으로 전체 구조물의 변위 형상을 예측할 수 있는 인공지능 학습기법을 개발하였으며, 개발된 기술의 성능을 실험을 통해 평가하였다. 3차원 공간에서 변위 형상 및 노드 위치 좌표의 특성을 학습에 반영할 수 있는 Image-to-Image 변위 형상 학습과 위치 특징을 결합한 변위 상관 학습 방법을 제시하였다. 개발된 인공지능 학습방법의 성능을 평가하기 위해 목업 구 조 실험을 진행하였고, 3D 스캔으로 측정한 변위값과 인공지능으로 예측한 결과를 비교하였다. 비교 결과 인공지능 예측 결과는 3D 스캔 측정 결과에 비해 5.6~5.9%의 오차율을 보여 적정 성능을 보였다.

This study compared the antioxidant and mineral properties of the leaves and seeds of fifteen Korean adzuki bean (Vigna angularis L.) breeding lines. This study was conducted in an attempt to expand the use of Korean adzuki bean leaves. The potassium, calcium, magnesium, and sodium contents of the leaves were significantly higher than the seeds, in particularly, the potassium content. The leaves had approximately 3.3 times higher potassium content than the seeds. For instance, the potassium content of YA1317 leaves was 21% higher than that of Arari. The total polyphenol content and ABTS activity of Adzuki bean leaves were significantly higher than the seeds, as opposed to the total flavonoid content and DPPH scavenging activity. Among the 15 breeding lines, YA1402 had 1.2~3.2 times higher antioxidant content and activity as compared to the Arari variety. It was concluded that adzuki bean leaves had higher mineral content, antioxidant component and activity as compared to the seeds. Therefore, adzuki bean leaves could be used an ingredient for dishes and as a medicine.

In vitro digestibility and protein digestibility corrected amino acid scores (PDCAAS) were investigated to verify the availability of protein in various Rhizopus oligosporus fermented products of domestic soybean (Glycine max L.) cultivars. Danbaegkong (DBK), Daepung (DP), Daewonkong (DWK), Saedanbaek (SDB), Seonyu (SY), and Cheongja4ho (CJ4) were used as raw samples, which were fermented using commercially available Rhizopus oligosporus for 48 h. All cultivars showed increased crude protein content after fermentation. The crude protein content of DBK and SDB was significantly higher than that of the other samples (55.12% in DBK and 54.22% in SDB) (p<0.001). CJ4 had the highest alanine content of 28.88 mg/g (p<0.001), and no significant difference in cysteine content was detected among the cultivars. In most of the fermented samples, the in vitro digestibility was 0.9 or higher, indicating high protein in the fermented samples. However, it is considered that restrictions on digestion are low. In DWK, the amino acid content and PDCAAS, which together indicate protein quality, were 0.917 and 0.855, respectively, confirming that it was the best cultivar to provide the raw material for fermentation. In conclusion Rhizopus oligosporus fermented soybean products can be considered a prospective source of protein with high utility value.

Barnea manilensis is a bivalve which bores soft rocks, such as, limestone or mudstone in the low intertidal zone. They make burrows which have narrow entrances and wide interiors and live in these burrows for a lifetime. In this study, the morphology and the microstructure of the valve of rock-boring clam B. manilensis were observed using a stereoscopic microscope and FE-SEM, respectively. The chemical composition of specific part of the valve was assessed by energy dispersive X-ray spectroscopy (EDS) analysis. 3D modeling and structural dynamic analysis were used to simulate the boring behavior of B. manilensis. Microscopy results showed that the valve was asymmetric with plow-like spikes which were located on the anterior surface of the valve and were distributed in a specific direction. The anterior parts of the valve were thicker than the posterior parts. EDS results indicated that the valve mainly consisted of calcium carbonate, while metal elements, such as, Al, Si, Mn, Fe, and Mg were detected on the outer surface of the anterior spikes. It was assumed that the metal elements increased the strength of the valve, thus helping the B. manilensis to bore sediment. The simulation showed that spikes located on the anterior part of the valve received a load at all angles. It was suggested that the anterior part of the shell received the load while drilling rocks. The boring mechanism using the amorphous valve of B. manilensis is expected to be used as basic data to devise an efficient drilling mechanism.

Bolboschoenus planiculmis has been acknowledged as a key species in whooper swans (Cygnus cygnus) habitat by providing food for this migratory waterfowl. B. planiculmis wetlands are being degraded by water shortages and salinization caused by anthropogenic activities and climate changes. In 2004, the distribution of B. planiculmis in the tidal flats of the Nakdong Estuary was 2,475,568 m2, and in 2021, the distribution area was 798,731 m2, which decreased by 32.3%. In order to restore the degraded B. planiculmis wetlands, shoot transplantation and seed sowing were tentatively used in three places with different salinity and water levels. The average density per unit area in September at the optimal growth period after planting were A (fresh water level 50 cm) 58±15.65 m-2, B (brackish water level 0~5 cm) 188±63.83 m-2, C (brackish water level 0 cm or less) 188±45.13 m-2. The tubers were observed as A 0 g dw m-2, B 25.32±2.94 g dw m-2, and C 13.39±0.91 g dw m-2. Tubers were distributed in the soil, with only 3.0% at the 10~20 cm depth but 97.0% at the 0~10 cm depth. In contrast, the germination rate of B. planiculmis seeds was observed to be 0%. Results of this study provide technical support for the restoration of B. planiculmis wetland and the improvement in the quality of whooper swans habitat.

To enhance the bioavailability and bioactivities of mixed herbal medicines (RW), they were fermented with lactic-acid bacteria isolated from kimchi into postbiotics (FRW). Then, from the results of the 16s rRNA sequencing analysis, lactic acid bacteria isolated from kimchi were identified to be of two species, namely Lactobacillus sakei and Leuconostoc mesenteroides. The FRW prepared from the RW were extracted using hot water (HW) and 70% EtOH (EtOH) for comparison of their macrophage-stimulating activities. Based on a comparison of the activities of the FRW extracts, nitric oxide (NO) production of HW was significantly higher than that in EtOH. An analysis of the chemical properties of the extracts showed that HW had higher contents of neutral sugar and uronic acid than EtOH as well as contained a large amount of glucose. In addition, crude polysaccharide (CP) was prepared to enhance the macrophage-stimulating activity. The FRW-CP not only secreted immunostimulatory mediators but also increased the expression of immunostimulatory genes (iNOS, TNF-α, MCP-1, and IL-6). The fractionated FRW-CP contained about 90% neutral sugars, and these sugars were mainly composed of glucose, galacturonic acid, and arabinose. Thus, FRW prepared by fermentation of RW with kimchi lactic acid bacteria were found to be immunostimulatory modulators.

한우 개량에 있어서 추정된 유전체육종가와 정확도는 선발에 중요한 지표로 사용되며, 최근 육종가 추정에 있어 정확도의 신뢰도를 높이기 위해 혈통과 유전체정보를 이용한 연구가 활발히 진행되고 있다. 따라서, 본 연구는 가계 내 유전체정보량에 따라 유전체정보가 미포함된 개체의 정확도 변화를 확인하고자 동일한 부모를 가진 한우 10두로 구성된 전형매 가계 3개를 수집하였으며, 각 가계 별로 유전체정보량을 10두, 8두, 6두, 4두, 2두씩 무작위로 선별하여 5가지의 검정집단으로 가정한 후 참조집단 14,225두를 이용하여 single step genomic best linear unbiased prediction (ssGBLUP)을 통해 genomic estimated breeding value (GEBV) 및 정확도를 추정하였다. 각 검정집단과 참조집단의 혈통 및 유전체정보 를 이용하여 H-matrix를 구축하였고, BLUPF90 program을 사용하여 도체중, 등심단면적, 등지방두께, 근내지방도의 GEBV 및 정확도를 추정하였 다. 첫 번째 가계를 대상으로 살펴보면, 검정집단에서 유전체정보를 보유하고 있는 10두의 GEBV 평균 정확도는 도체중 0.734, 등심단면적 0.717, 등지방두께 0.712, 근내지방도 0.745로 추정되었다. 이후 2두씩 무작위로 유전체정보를 제거하여 추정한 GEBV 정확도를 살펴보면, 유전체정보를 보유한 개체의 경우 정확도의 변화가 나타나지 않았지만, 유전체정보가 미포함된 개체의 정확도가 평균 0.114 ~ 0.168 낮게 추정되었다. 가계 내 유전체정보량에 따른 유전체정보가 미포함된 개체의 GEBV 평균 정확도는 도체중 0.604 ~ 0.576, 등심단면적 0.6 ~ 0.573, 등지방두께 0.599 ~ 0.572, 근내지방도 0.607 ~ 0.578로 평균 0.009씩 감소하는 것을 확인하였다. 이를 통해 가계 내 유전체정보량이 개체 별 유전체정보 유무와는 상관없이 GEBV의 정확도 추정에 큰 영향이 없었으며, 신뢰도가 높은 GEBV 추정을 위해서는 개체 별 유전체정보의 유무가 더 큰 영향을 미친다는 것을 확인하였다.

Purpose: This study aimed to explore nursing simulation training needs and the educational environment of instructors. Method: This study was conducted according to the inductive method of qualitative content analysis. An online focus group interview was conducted with six instructors with experience operating a nursing simulation. Data were collected in July 2021. Results: First, “nursing simulation practice operations” required simulation design elements, practical operations, and curriculum integration. Second, the “educational environment” showed a lack of human and physical resources, and there was a need for standard education reflecting the domestic nursing education environment. Third, for “instructor competencies”, the facilitator role, step-by-step education for strengthening instructor competencies, and cooperation with clinical experts were derived. Fourth, it was found that “learner competencies” should meet the nursing professionals’ needs; self-directed learning competency and unique human competency should also be strengthened. Conclusion: This study is meaningful because it seeks to understand the instructor’s needs for nursing simulation training and the educational environment. Moreover, it identifies considerations for instructor and learner competencies in the nursing simulation operator training programs.