매실은 식이섬유, 비타민, 칼륨, 각종 유기산을 풍부하게 함유하고 있어 음료, 절임 등 각종 가공식품으로 개발되어 있으며 그 수요가 점차 증가하고 있다. 그러나 매실에 함유되어있는 시안배당체의 일종인 amygdalin은 체내 존재하는 β-glucosidase 효소에 의해 시안화물 대사체를 생성할 수 있으며, 이는 호흡 저하 등의 독성을 유발할 수 있는 것으로 알려져 있다. 따라서 소비자의 매실가공제품에 대한 안전성 우려가 높아지고 있으나, 독성에 관한 체계적 연구는 미진한 편이다. 따라서 본 연구에서는 amygdalin의 세포독성을 세포생존율, 산화스트레스 및 콜로니 형성능에 미치는 영향을 확인하여 연구하였으며, 약동학적 연구를 통하여 amygdalin 자체의 체내 흡수율을 확인하였다. 아울러 매실가공제품인 매실청의 약동학적 연구 및 반복경구독성 연구를 수행하여 독성영향을 비교하였다. 세포 독성 결과, amygdalin 자체의 독성은 나타나지 않았으나, β-glucosidase 효소와 동시처리 시 대사체 생성에 의한 독성이 증가하였으며, 약동학적 연구 결과 체내 흡수율은 매우 낮은 것으로 확인되었다. 또한 매실청의 반복투여에 의한 독성영향도 관찰되지 않았다. 본 연구 결과는 amygdalin, 대사체 및 매실가공제품의 안전성에 관한 중요한 정보를 제공함으로써 매실산업 발전에 기여할 수 있을 것이다.

육계(Cinnamomum cassia Presl)의 물 추출물이 알코올대사 효소인 alcohol dehydrogenase (ADH)와 aldehyde dehydrogenase(ALDH)의 활성에 미치는 영향을 확인하였다. 육계 추출물은 100 μg/ml의 농도에서 ADH의 활성을 98.8% 저해하였고, ALDH의 활성은 32.5% 저해함으로써 ALDH에 비해 ADH에 대한 저해효과가 탁월하였다. 육계를 80℃의 물로 2시간 이상 추출하였을 때 ADH의 저해 효과가 가장 높았다. 육계 추출물은 37℃, pH 2의 조건에서 4시간 이상 방치하여도 ADH의 저해 효과가 거의 100% 유지되어 ADH 활성 저해 물질은 산에 매우 강한 것으로 나타났다. 또한 100℃의 물에서 2시간 끓여도 ADH 저해활성이 92.8% 유지되어 육계 추출물의 ADH 활성 저해 물질은 열에도 매우 강한 것으로 나타났다. 육계 추출물을 열풍건조 및 감압농축 과정을 거치고 나면 ADH 저해활성이 사라지는 것으로 보아 육계 추출물의 ADH 활성 저해 물질은 쉽게 증발하는 휘발성 향기성분으로 판단된다. 향후 육계 추출물의 ADH 활성 저해물질의 구조와 활성저해 메커니즘을 규명함으로써 육계 추출물의 숙취해소 천연소재로의 활용을 기대할 수 있다.

This study, analyzed the general and minerals composition of kamut and investigated its effect on blood components in mice fed a high fat diet. The content of each general component of kamut was as follows: 11.02±0.75% water, 13.16 ±1.28% crude protein, 1.85±0.19% crude fat, and 1.97±0.13% ash. The leptin level was the highest in the HF group(30.00± 0.00 ng/mL) when compared to the control group. There was a significant decrease of 23.65±5.54 ng/mL in the HFK group when compared to the HF group (p<0.05). The blood LDL-cholesterol concentration was the lowest in the control group at 10.00±2.00 mg/dL. The level was highest in the HF group at 28.00±0.00 mg/dL when compared with the other groups (p<0.05). The aspartate transaminase (AST) level was significantly higher in the HFK group (179.33±173.88 U/L) than in the control (61.00±12.73 U/L) and HF groups (132.00±0.00 U/L). According to the results of this study, the consumption of kamut lowers the blood LDL-cholesterol level more than the consumption of wheat flour. Additionally, kamut contains antioxidant substances such as selenium and zinc, which are thought to contribute to vascular health and thus aid in maintaining good health. Therefore, it is necessary to develop a variety of health foods using kamut; it should be used as a functional food for the maintenance of good health.

본 연구는 대사증후군을 가진 65세 이상 노인여성을 대상으로 마루운동을 실시하여 노인활동체력, 혈중지질 및 동맥경직도에 미치는 영향을 조사하였다. 대사증후군을 가진 여성노인 41명을 무작위로 운동군(23명), 통제군(18명)으로 배정하였다. 운동군은 총 12주간 주 3회로 실시하였으며, 운동강도는 30%-60%의 HRR로 점진적으로 증가시켰다. 본 연구결과, 운동군이 노인활동체력(하지근력, 상지근력, 유연성, 심폐지구력 및 신체조성(체지방률, 제지방량, 허리둘레)), 혈중지질(총 콜레스테롤, 중성지방, HDL 콜레스테롤 및 LDL 콜레스테롤) 및 동맥경 직도에서 실험 전·후 긍정적인 변화를 보였고, 통제군에 비해 유의한 차이를 나타냈다. 결과적으로 대사증후군을 가진 여성노인들은 마루운동 프로그램 참여에 따라 노인활동체력이 향상되고, 혈중지질과 동맥경직도에 긍정적인 변화가 일어났다. 따라서, 마루운동프로그램은 대사증후군 개선에 효과적인 프로그램임을 시사한다.

본 연구는 차세대 랫드에서 오메가 6(n-6)와 오메가 3(n-3) 지방산 비율이 서로 다른 식이 (0, 1.2:1, 8:1, 19:1)의 섭취가 간에서 새롭게 합성․분비되는 glycerolipid의 대사적 분할에 관한 메카니즘을 구명하였다. 그 방법으로써 경정맥 캐뉼라를 부착한 랫드에서 생체모니터링 기법을 이용하였다. glycerolipid 대사에 의한 산화로써 나타난 14CO2 배출율 범위는 8:1 이하 그룹에서 7.05-20.17%로 나 타났으며 대조구에서 가장 낮았다(p<0.05). 총 glycerolipid는 대조구가 가장 높았으며 19:1, 8;1, 1.2:1 그룹의 순서로 높았다(p<0.05). 중성지방 분비량은 대조구와 비교할 때 1.2:1, 8:1, 19:1 그룹의 순서로 각각 36.35, 20.93, 13.72% 감소하였다(p<0.05). 총 glycerolipid에 대한 인지질의 분할 비율은 대조구와 비교할 때 1.2:1, 8:1, 19:1 그룹의 순서로 각각 1.38, 1.29, 1.17배 높았다(p<0.05). 총 glycerolipid에 대한 14CO2 생성비율은 대조구와 비교할 때 1.2:1, 8:1, 19:1 그룹의 순서로 각각 1.61, 1.52, 1.29배 높았다(p<0.05).

임신 전 젖소의 육성기 후반에 있어서 적정한 에너지와 단백질 급여 관리는 개체의 성장과 분만 후 유생산에 영향을 미친다. 본 연구는 육성기 후반의 적정 TDN:CP 비율을 결정하기 위하여 생후 15개월 령에 5.0:1(T1), 4.5:1(T2) 및 4.0:1(T3)의 비율로 급여하여 체중, 사료섭취량 및 혈액조성에 대하여 조 사하였다. 건물섭취량은 평균 8.07kg/day로 TDN: CP 비율에 관계없이 유사하였다. 단백질 섭취량은 TDN:CP 비율이 낮아짐에 따라 각각 1.45, 1.29 및 1.17kg/day로 감소하였으나 유의적 차이를 보이지 않았다. 증체량은 4.0:1 비율구가 유의적으로 가장 많이 증가하였으며, 평균 일당증체량도 0.74kg/day 로 가장 높게 나타났다(p<0.05). 60일 이후 모든 처리구의 일당증체량이 권장 수준을 충족하였으나, 4.0:1 비율구(1.28kg/day)에서 과잉 체지방 축적이 될 수 있는 수준이기 때문에 임신 및 번식과 관련하 여 주의해야 한다. 육성우 15~18개월령에서 상기의 모든 TDN:CP 비율은 혈액 조성에 큰 영향을 미치 지 않았다. 체중과 출산 및 비유 등을 고려했을 경우 4.5:1에서 5.0:1의 비율이 적합하고, 출산 시기가 가까울수록 에너지 단백질 비율을 5.0:1까지 높여주는 것이 바람직할 것으로 판단되었다.

한국의 가양주는 지역별로 그 특징이 다르게 나타나며, 누룩균은 이러한 지역별 가양주의 특징을 결정 짓는데 가장 중요한 요소이다. 본 연구에서는 국내 지역별(충남, 서울, 제주) 가양주에서 분리한 누룩균 배양체의 대사체 분석을 통해, 균주별 특성을 비교 분석하였다. 총 7 지역의 가양주에서 분리된 20종의 누룩균 배양체의 대사체를 용매(Isopropyl alcohol: Methanol: Distilled water)추출 한 후, LC-MS/MS를 이용해 대사체 분석을 수행하였다. 이후 다변량 통계에 기반한 data mining을 통해 결과들을 분석하여 지역 및 균주별 대사체 변이를 규명하였다. Mass spectrometry에서 검출된 300여개의 전체 대사체들은 지역 및 균주별 특이성에 따라 분류 될 수 있었으며, Aspergillus niger 계열 균주들에서는 gluconic acid의 함량이 특이적으로 높게 나타났다. 이러한 지역 및 균주별 대사체의 변이 기여하는 유의적인 대사체들은 약 36개로 screening 되었고, 이들의 조성과 함량에 의해 지역별 가양주들의 특성이 발현되는 것을 확인할 수 있었다. 본 연구 결과는 향후 전통 가양주 유래 유용 미생물 자원의 발굴에 유용한 기초 자료로 활용될 수 있을 것으로 기대된다.

The purpose of this study was to compare nutrient intake, health-related factors, and risk factors for metabolic syndrome and to investigate the prevalence odds ratio according to fruit and vegetable intake among middle-aged Korean men. Subjects included 1,677 men aged 40–64 years. The average intake of fruits and vegetables was 578.48±5.90 g/day. Forty-nine percent of the subjects were categorized into the deficient group based on the intake of fruits and vegetables, and the other subjects were categorized into the adequate group. There was a significant difference in the nutrient density per 1,000 kcal of energy, minerals, vitamins, and dietary fiber between the fruit and vegetable intake groups (p<0.001). In terms of health-related factors, only the current smoking rate in the deficient group (57.9%) was higher than that in the adequate group (45.4%) (p<0.001). There was no significant difference in the risk factors for metabolic syndrome between the fruit and vegetable intake groups. Proportion of serum triglycerides (42.6%) was the highest at a rate that exceeded the criteria for risk factors for metabolic syndrome, but systolic blood pressure (21.7%) showed a very low rate. The fasting blood glucose, serum triglycerides, and diastolic blood pressure were significantly higher in the deficient group than in the adequate group (p<0.05). Prevalence of metabolic syndrome was 31.2%. OR for prevalence of metabolic syndrome related to fruit and vegetable intake was 1.270 times higher in the deficient group than in the adequate group (p<0.05). Also, when the daily fruit intake was increased by 100 g, the prevalence of metabolic syndrome was reduced by 0.948 times but it was not related to the vegetable intake. The results of this study can be used as the basic data to establish the guidelines for fruit and vegetable intake for prevention of metabolic syndrome in middle-aged men.

This study aimed to investigate growth rate and nutrient consumption of Chlorella vulgaris according to different light irradiation interval. Applied light irradiation intervals were 12 hr, 4 hr, 1 hr, and 1 min. The light source was flexible LED(Blue:Red=1:1), light intensity was 200 PPFD and Light/Dark cycle was 1:1. As a result, growth rate and nutrient removal efficiencies showed no significant differences depending on the light irradiation interval. Considering the reproduction characteristics of applied microalgae cultures of this study, this is thought to be one of the possible reasons of above results. Because Chlorella vulgaris performs an asexual reproduction and it is known that there is no significant relationship between light irradiation interval and growth rate, including nutrient consumption in case of asexual reproduction.

본 연구는 열 스트레스 하에서 오리사료 내 대사에너지(ME) 수준이 오리의 간, 십이지장 융 모, 미생물, 유전자 조절에 미치는 영향을 조사하였다. 총 240마리의 육용 오리 채리밸리(Anas platyrhynchos)를 4처리구로 완전임의배치 한 후 42일 동안 사육하였다. 처리구는 ME 2900 kcal/kg, ME 3000 kcal/kg, ME 3100 kcal/kg 및 ME 3200 kcal/kg로 구분하였다. 간 조직은 처리구 사이의 차이가 없었고, 십이지장 융모 및 창자샘 길이는 ME 3000과 비교할 때 2900은 10.58% 감소하였으나 3100, 3200과의 사이에 차이는 없었다. 맹장 Latobacillus는 ME 3000과 비교할 때 2900은 9.47% 감 소하였으나 3100, 3200은 각각 2.52, 3.24% 증가하였다. Total aerobic bacteria, E. coli, Coliform bacteria는 ME 3000과 비교할 때 2900은 증가하였으나 3100, 3200은 차이가 나타나지 않았다. 간에서 HSP (heat shock proteins)-mRNA 중 HSP 90-α는 ME 3000과 비교할 때 2900은 48.60% 감소하였 으며 3100, 3200은 차이가 없거나 증가하였다.

The purpose of this study was to investigate the effect of rope skipping and band exercise on GH, IGF-1 and metabolic syndrome risk factors in female obese middle school students. Twenty female obese middle school students(%BF > 30%) were randomly assigned to a exercise group(EX, n=10) or control group(CON, n=10). The exercise group has performed rope skipping and band exercise for 12 weeks, 3time per week. Exercise intensity was increased gradually, from 40% to 70% of Heart Rate Reserve(HRR) and Rating of Perceived Exertion(RPE) 11-15. GH, IGF-1 and metabolic syndrome rist factors were GH, IGF-1 and metabolic syndrome risk factors were measured pre- and post-exercise program including rope skipping and band exercise. Levels of GH and IGF-1 were significantly increased (p<.01 and p<0.001, respectively) in exercise group after 12 weeks of exercise training. WC and TG were significantly decreased(p<.01) in exercise group after 12 weeks of training. HDL-C was significantly increased(p<.05) in exercise group after 12 weeks of training. In conclusion, rope skipping and band exercise were effective exercise program in improving GH, IGF-1 and metabolic syndrome risk factors in female obese middle school students.

This study was conducted to certify the effect of aqueous extracts from fifty medicinal plants on the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in vitro. Each aqueous extract was prepared by combining one-part medicinal plants with twenty-parts distilled water at 80°C for 8 h. Among the fifty medicinal plants, Allium sativum L. and Cinnamomum cassia Presl were regarded as an effective anti-hangover substance. Allium sativum L. extract increased ALDH activity more than 2 times compared with ADH activity, enhancing the acetaldehyde degradation. Cinnamomum cassia Presl extract dramatically inhibited ADH activity compared with ALDH activity, thus potently decreasing the acetaldehyde formation. ADH and ALDH activities were proportionally inhibited according to the increased concentration of Cinnamomum cassia Presl extract. The aqueous extract of Cinnamomum cassia Presl at a concentration of 45.33 μg/mL inhibited ADH activity by 52.8% and ALDH activity by 11.0%.

This study was conducted to investigate if the supplement formula may improve alcohol metabolism in healthy adult men.In a double-blinded, randomized, crossover study, subjects were administrated yeast extract mixtures (YEM, n=15), Hovenia dulcis mixtures (HDM, n=15), placebo (PLA, n=15), and control (CON, n=15) in an oral dose followed by one week washout periods. At each visit (0, 1, 2, 3, 4 week), subjects drank 450 mL, 20.1 percent alcohol after administered mixtures. Blood was drawn periodically (0, 0.25, 0.5, 1, 2, 4, 6, 15 hours). Fifteen subjects completed the protocol and were included in the analysis. Plasma ethanol concentration was lower in YEM (10 percent) and the HDM (5 percent) groups. The area under the curves (AUC) and Cmax for plasma ethanol were significantly decreased only in the YEM group, when compared with the CON group. The AUC and Cmax for plasma acetaldehyde concentration were significantly decreased in the YEM (45 and 54 percent) and the HDM (35 and 53 percent) groups respectively, when compared with PLA (p<0.01). Together, these findings validate that YEM or HDM improved alcohol metabolism and hangover syndromes, leading to reduce alcohol concentration and acetaldehyde concentration without adverse effects.

Although Semisulcospira libertina is generally regarded as a supplement for the alleviation of alcohol hangover, little is known about its effects on cell metabolism. Therefore, this study was conducted to analyze the constituents of the extracts prepared using different extraction methods and to compare their biochemical properties. The amino acid contents were found to be much higher in acidic and enzymatic hydrolysates than hot water extracts from S. libertina. DPPH radical scavenging activities in acidic and enzymatic hydrolysates were higher than those of hot water extracts. Three types of S. libertina hydrolysate was added to HepG2 cells damaged by acetaminophen (AAP), after which the survival rate of HepG2 cell were measured. In addition, lactate dehydrogenase (LDH) activities in the culture media were evaluated. The survival rates of HepG2 cells were 77.0±4.3% and 81.5±1.3% at 3 h and 5h enzymatic hydrolysates, respectively. These cell survival rates were higher compared to those of the negative control group (67.8±4.3%) treated only with acetaminophen. Cellular toxicities induced by treatment with AAP were also significantly alleviated in response to treatment with the extracts of S. libertina. In addition, the activities of 2 key enzymes that metabolize ethanol, alcohol dehydrogenase and aldehyde dehydrogenase, were upregulated by 4.7- and 2.7-fold respectively in response to treatment with a 3 h enzymatic hydrolysate of S. libertina. Taken together, these results provide biochemical evidence of the method by which S. libertina exerts its biological functions, including the alleviation of alcohol hangover and the protection of liver cells against toxic insults.

Although Semisulcospira libertina is generally regarded as a supplement for the alleviation of alcohol hangover, little is known about its effects on cell metabolism. Therefore, this study was conducted to analyze the constituents of the extracts prepared using different extraction methods and to compare their biochemical properties. The amino acid contents were found to be much higher in acidic and enzymatic hydrolysates than hot water extracts from S. libertina. DPPH radical scavenging activities in acidic and enzymatic hydrolysates were higher than those of hot water extracts. Three types of S. libertina hydrolysate was added to HepG2 cells damaged by acetaminophen (AAP), after which the survival rate of HepG2 cell were measured. In addition, lactate dehydrogenase (LDH) activities in the culture media were evaluated. The survival rates of HepG2 cells were 77.0±4.3% and 81.5±1.3% at 3 h and 5h enzymatic hydrolysates, respectively. These cell survival rates were higher compared to those of the negative control group (67.8±4.3%) treated only with acetaminophen. Cellular toxicities induced by treatment with AAP were also significantly alleviated in response to treatment with the extracts of S. libertina. In addition, the activities of 2 key enzymes that metabolize ethanol, alcohol dehydrogenase and aldehyde dehydrogenase, were upregulated by 4.7- and 2.7-fold respectively in response to treatment with a 3 h enzymatic hydrolysate of S. libertina. Taken together, these results provide biochemical evidence of the method by which S. libertina exerts its biological functions, including the alleviation of alcohol hangover and the protection of liver cells against toxic insults.