암, 당뇨, 심장질환 등의 성인병 예방에 좋은 효과를 제공하기 때문에 선진국에서는 통곡의 섭취를 권장하고 있으나, 곡물이 겨층을 포함하고 있어 식미가 거친 단점이 있다. 초록통쌀은 호숙기에 수확하여 조직이 경화되지 않고 각종 미네랄, 비타민, 식이섬유 등 영양성분이 풍부한 것으로 알려져 있다. 본 연구는 고령자의 영양보충 및 식감 개선을 위한 초록통쌀 현미의 영양성분 및 효소처리에 따른 이화학적 특성변화를 분석하였다. 국내 다 소비품종인 삼광, 조평, 추청 3종의 초록통쌀의 영양성분을 비교 분석하였으며, 4종의 효소 처리(0.1 ~ 3%)에 따른 신속호화점도계, 전자현미경, 아밀로펙틴 함량 등의 이화학적 특성 변화를 측정하였다. 삼광, 조평, 추청의 주요 영양성분의 함량은 100g 당 비타민 B1이 144, 100, 105ug, 식이섬유가 21, 13, 12g, 폴리페놀이 63, 52, 56mg으로 삼광이 가장 높은 것으로 확인되었으며, 비타민 B2, 나이아신, 칼륨, 인, 마그네슘, 총 엽록소도 높은 것으로 나타났다. 효소농도가 증가함에 따라 경도가 감소하였으나, 0.3% 이상에서는 유의적 차이가 없는 것으로 나타났다. 대조군 및 각 효소처리에 따른 식미적 합성을 나타내는 신속호화점도계 peak 값은 760, 724, 708, 754, 761 cP이었고, 시간변화에 따른 품질특성을 나타내는 Breakdown 값은 157, 166, 151, 100, 99 cP로 두 항목 모두 셀룰로오스계열 효소 D 처리 시 식미가 가장 우수한 것으로 확인되었다. 전자현미경 확인결과 효소처리에 따른 물성변화로 입자간 간극이 커짐을 확인하였다. 전분의 분자량 분포특성인 아밀로펙틴 측쇄사슬 길이는 모든 처리군 평균값이 15.71~15.99 DP로 효소처리에 따른 아밀로펙틴 함량 변화는 없는 것으로 나타났다. 이러한 결과는 셀룰로오스 계열 효소처리를 통하여 물성조절이 가능하고 식미도 우수한 것으로 나타나, 초록통쌀은 저작능력이 약한 고령자가 쉽게 섭취할 수 있고, 영양개선을 위해 활용할 수 있음을 제시하고 있다.

Ceramic membranes can be applied under extreme operating conditions such as low pH, high pressure and high temperature. In particular SiC has excellent mechanical properties and also has excellent properties related to membrane performance. However, high processing temperature increases cost of SiC products and thus limit’s its use. In this study oxidation bonding technique was used to fabricate cost-effective SiC microfiltration membrane at low temperature. The oxidation behavior at different thermal treatments was related with pore morphology and ultimately the membrane permeability. We have found that the membrane made by coating of oxidation bonded SiC layer over clay-bonded SiC support, sintered at 1000-1100°C could make a defect-free microfiltration membrane with pure water permeability above 700 LMH per bar. The membrane has narrow pore size distribution with average pore size about 0.1 μm.

Oxidation of erythromycin, sulfamethazine and sulfathiazole by ozone was experimentally investigated to see the effects of background water quality such as ultrapure water, humic acid and biologically treated wastewater and water temperature on the removal rate, consequently to provide design information when the ozone treatment process is adopted. Initial concentration of the antibiotics was spiked to 10 μg/l and ozone dose was 1, 2, 3, 5, 8 mg/l. While the removal rate of erythromycin under ultrapure water background by ozone oxidation was over 99%, that under humic acid and biologically treated wastewater background was markedly reduced to the range of 59.8%~99% and 17.0%~99%, respectively. When water temperature is decreased from 20℃ to 4℃, the removal rate is reduced from the range of 17.0%~99% to the range of 9.4%~97.4% under biologically treated wastewater background. The effects of background and temperature on the removal rate of sulfamethazine and sulfathiazole were similar to erythromycin, but the degree was different. Therefore, it is concluded that the background of water to be treated as well as water temperature should be taken into consideration when the design factor such as ozone dose is determined to meet the treatment objective in the ozone treatment process.

돼지 난자의 체외성숙 배양액에서 다양한 농도의 육탄당 처리는 난자의 성숙률과 배 발 육능, 세포수 증가에 영향을 주는 것으로 보고되고 있다. 본 연구에서는 육탄당의 일종인 fructose가 돼 지 난자의 체외성숙에 미치는 영향을 조사하였다. 난자의 체외배양액으로는 cysteine, pyruvate, epidermal growth factor, kanamycin, insulin 및 호르몬이 첨가된 porcine zygote medium (PZM)-4 또는 10% 돼지 난포액이 첨가된 PZM-3을 이용하였고 실험설계에 따라 다양한 농도의 fructose 및 5.5 mM의 glucose를 첨가하여 44시간 동안 배 양함으로써 난자의 성숙을 유도하였다. 첫 번째 실험에 서는 1.5, 3.0 및 5.5 mM의 fructose 와 5.5 mM의 glucose가 첨가된 성숙배양액에서 배양된 난자의 성숙률 및 단위발생 후 배 발육능을 조사하였다. 실험 결과 1.5, 3.0 및 5.5 mM fructose를 첨가한 배양액에서 성숙된 난자는 5.5 mM glucose가 첨가된 배양액에서 성숙된 난자와 비교하였을 때 유 사한 핵 성숙 률(81.9-91.5% vs. 94.2%), 단위발생 후 분할률(89.4-92.4% vs. 90.1%), 배반포 발달률 (39.3-41.1% vs. 39.4%) 및 배반포 세포수(30.8-34.2% vs. 31.8%)를 보였다. 두 번째 실험에서, 10% 돼지 난포액이 첨가된 PZM-3 배양액에서 무처리 및 3.0 mM fructose, 5.5 mM glucose 및 3.0 mM fructose와 5.5 mM glucose 병행 첨가 성숙배양액에서 성숙된 난자의 성숙률 및 단위발생 후 배 발 육률을 조사하였다. 실험 결과 3.0 mM fructose (93.1%), 5.5 mM glucose(91.7%) 및 3.0 mM fructose 와 5.5 mM glucose 병행 처리군(93.5%)이 무처리군(74.4%)에 비해 유의적으로(P<0.05) 높은 핵 성 숙률을 보였다. 또한 5.5 mM glucose(90.1%) 및 병행 처리군(97.2%)은 대조군(67.6%)에 비해 유의 적으로(P<0.05) 높은 분할률을 보였다. 배반포 발달률은 3.0 mM fructose(52.6%) 및 병행 처리군 (58.8%) 에서 무처리군(44.4%) 및 5.5 mM glucose (51.0%)에 비해 유의적으로(P<0.05) 증가하였다. 본 실험 결과로 보아 체외성숙 합성배양액 내 fructose 첨가는 glucose와 유사한 수준의 배 발육률 을 보임으로써 glucose를 대체할 수 있는 에너지원으로 이용 가능함을 확인하였다.

목적 : 본 연구의 목적은 복지 사회가 요구하는 작업치료서비스의 질 관리와 역량 있는 작업치료사를 양성 하기 위하여 국내 실정에 맞는 한국작업치료인증기준 평가항목을 개발하는 것이다. 연구방법 : 국내 작업치료학과 교수를 대상으로 2016년 6월부터 8월까지 한국작업치료인증기준 평가항목 의 타당성을 조사하였다. 평가항목은 문헌고찰 및 자료조사를 통하여 작업치료교육인증기준에 대한 6개 영역으로 구성하였으며, 항목의 타당도는 4점 척도를 사용하여 측정하였다. 1차 조사를 통하여 회수된 84부의 타당도 분석결과를 바탕으로 포커스집단 회의를 통해 내용을 재검토하고 수정 보완하여, 작업치 료 핵심실무역량 평가요소 및 항목을 포함하여 최종 한국작업치료인증기준 평가항목을 구성하였다. 최종 평가항목은 작업치료교육전문가 10명을 대상으로 내용타당도를 조사하였다. 결과 : 한국작업치료인증기준 평가항목은 총 6개 영역, 15개 부문, 31개 하위항목으로 구성하였으며, 항목 별 타당도 평균은 3.3~4.0점까지의 범위로 최종 내용타당도 지수(Content Validity Index; CVI)는 0.8~1.0까지로 나타났다. 작업치료 핵심실무역량 평가항목은 상담/평가 및 중재/기타 2개 영역, 21개 하위항목으로 분류하였으며, 타당도 평균은 3.0~3.6점까지의 범위로 CVI값은 1.0이었다. 핵심실무역량 평가 방법으로는 실제 테스트가 상담/평가에서는 40.6%, 중재/기타에서는 37.5%로 가장 높게 나타났다. 결론 : 한국작업치료인증기준을 확립하고 적용하여 역량 있는 작업치료사를 양성할 수 있는 교육시스템을 권장하고, 국내 모든 대학의 작업치료 교육의 질을 향상시킴으로써 작업치료서비스의 국제적 호환성 및 사회적 책무성을 달성할 수 있어야 할 것이다.

미리찌 증후군은 담낭절제술을 받은 환자들 중 2% 이하를 차지하는 드문 질환이다. 담낭관 변이와 동반된 미리찌 증후 군의 경우는 수술 중 변이의 발견이 어렵고, 수술 후 합병증이 높아 수술적 치료에 어려움이 있다. 본 증례는 과거 복강경 담 낭절제술을 받은 상태에서, 담관염 소견으로 방문하여 저위 합류 담낭관의 잔여 담낭관에 담석이 감돈되어 발생한 미리 찌 증후군을 진단하고, 내시경으로 성공적으로 치료한 사례 이다. 저위합류 담낭관과 같은 해부학적 변이를 동반한 미리 찌 증후군의 경우 수술적 치료가 어렵거나, 불완전할 수 있다. 이에 내시경 역행담췌관조영술을 통한 인공배액관 삽입술 및 UDCA 약물요법 이후 풍선확장술을 이용한 담석 제거술이 수술적 치료의 대안 치료로 고려될 수 있다.

The purpose of the study was to simplify standard HACCP manual for improving the on-site applicability and increasing the implementation rate of HACCP program for small- and medium-sized dairy farms (SMDF). A survey was carried out in 32 SMDFs using a structured questionnaire. The results indicated that the number of workers had a positive correlation with HACCP certifications (p < 0.05). Most of HACCP non-certified farms (66.7%) were run by two workers with 40-60 cows and milking yields of 1,000-1,500 L per day. Major drawbacks for dairy farmers to implement current HACCP system were the difficulties in daily recording and facility management (40%). On the basis of the survey results, it was suggested that the development of on-site standard HACCP manual and user-friendly record chart with O/X checklist were the most urgent factors to increase HACCP implementation rate. In addition, the alternative third party service for microbial and chemical tests could also be accompanied for small-sized dairy farms.

Magnesium alloy is becoming known for the lightest material in the metallic materials. Recently the automotive industry has a variety application to the light weight parts replacement. This study focuses on the mechanical property improving through a tiny amount’s CNT addition into the magnesium alloy as AM60. The CNT material is an arduous combination of the metallic materials. Therefore this study is concentrating on the contact force growth for the CNT material. Consequently, the made CNT is produced by the CVD process using the magnesium catalyst. The CNT material has dispersive with mechanical process into the molten AM60 alloy. The mechanical experiment result that hardness is 18% increasing and tensile strength is 13% increasing, better than the raw AM60 alloy on this investigation.

국내 경주마의 승용마로 전환에 있어서 가장 큰 문제 중 하나인 침착하고 온순한 성격의 말 을 조기에 판정이 필요하고, 특히 crib biting같은 행동을 보이는 사나운 성격의 말을 조기에 제 거하는 선발 기술이 필요하다. 본 연구에서는 성격적으로 민감하고 난폭한 그룹과 온순한 성격 의 말을 구분 지을 수 있는 유전적 특성을 파악하고자 연구를 수행하였다. 말의 성격적 특성을 구분 지을 수 있는 유전적 변이를 찾기 위해서 기존에 포유동물의 성격적 특징과 관련이 있는 것으로 알려진 유전자 후보군 71개를 선발하였다. 71개 유전자 중, 말 참조 유전체에 이미 존재 하는 16개 유전자와 인간 참조 유전체를 기반으로 말에서 찾은 orthologous gene 17개를 합하여 총 33개 성격관련 유전자를 선정하였다. 선정된 33개 유전자의 exon 부분에 probe를 제작하여 목표로 하는 유전자만 추출한 후 Illumina Hiseq2500을 이용하여 whole exome sequencing을 진 행하였다. Whole exome sequencing 결과로 얻어진 데이터를 이용하여 sanger validation기법을 통해 SNPs분석을 실시하였다. 그 결과 33개의 유전자 중 androgen recptor gene (AR)과 dopamine receptor D4 gene (DRD4)유전자에서 유전적으로 성격에 차이가 크게 나타난 결과를 확인할 수 있었다. 이러한 유 전자를 활용한다면 말 사육 농가의 조기 말 성격 검사를 통하여 사육 방향을 선택할 수 있는 경 제적 이득을 취할 수 있을 것으로 예측되며, 향후 추가적인 유전자 분석이 완료 된다면 현장에서 신속하게 사용할 수 있는 키트 개발등이 가능할 것으로 기대된다.

Identification of specific marker proteins in cells is useful for isolating cells and determining their cellular characteristics and functions. Based on our previous study showing that matrix metalloproteinase 9 (MMP-9) can be used as a marker for porcine spermatogonia, the expression pattern of MMP-9 was determined in both pre- (5-month old) and post-pubertal (11–month old) bovine testes. Histological analysis revealed that spermatogonia were located near the basement membrane in both testes, while spermatozoa were not detected in the 5-month old pre-pubertal bovine testes and epididymides. Mature spermatozoa were observed in the 11-month bovine testes and epididymides, and MMP-9 expression in 11-month old bovine testes was lower than 5-month old testes, according to reverse transcription-PCR and real-time-PCR data. To determine the specific expression sites of MMP-9 in the bovine testes, immunohistochemistry was performed. Expression of MMP-9 was observed in cells near the basement membrane of seminiferous tubules in both 5- and 11-month old testes. Furthermore, MMP-9 positive cells expressed protein gene product 9.5 (PGP9.5) and deleted in azoospermia (DAZL) that are already known as bovine spermatogonial stem cells markers. In the present study, MMP-9 expression was identified in both pre- and post-pubertal bovine spermatogonia expressing PGP9.5 and DAZL, and located near the basement membrane of seminiferous tubules. Thus, MMP-9 can be used as a marker for bovine spermatogonia, and may provide useful platforms for understanding the interaction between germ cells and extracellular matrix during spermatogenesis in the seminiferous tubules.

The characteristics of CNT-Polyamide composites were analyzed, that is, tensile strength, electrical resistivity, and thermal conductivity were measured according to the align length of CNT. There have been researches on the influence of aligned CNT to improve the mechanical and thermal characteristics in different areas including absorption and shielding of electromagnetic wave, thermal distribution or absorption, and high-strength of CNT. The aligned CNTs were synthesized by the ethylene gas with a CVD device preheated at 650℃. CNT-Polyamide composites were produced with the mixing of solution. CNT contents were controlled from 1phr to 50phr in the polyamide-ethanol solution, and blended with the 700W bar-type ultrasonic wave for 60 min.. And then CNT-polyamide were precipitated by CNT-polyamide-etnanol falling into the cold water. After dried 12 hours, CNT-polyamide composite were pressed at 150℃~180℃ with 400kgf to get the thickness of 1mm. As the conclusions, aligned CNT bundles were dispersed by cutting of CNT to the aligned direction because of polyamide properties. Tensile strength and electrical resistivity were improved to the increase of aligned length of CNT. Thermal conductivity was little affected by the align length of CNT.

As supply chains are globalized, multinational companies are trying to optimize distribution networks using a hub and spoke structure. In this hub and spoke network structure, multinational companies locate regional distribution centers at hub airports, which serve demands in their corresponding regions. Especially when customers put higher priority on the service lead-time, hinterlands of international hub airports become ideal candidate locations for the regional hub distribution centers. By utilizing excellent airport and logistics services from hub airports, regional distribution centers in the hub airports can match supply with demand efficiently. In addition, regional hub distribution centers may increase air cargo volume of each airport, which is helpful in the current extremely competitive airport industry. In this paper, we classified locational preferences into three primary categories including demand, service and risk and applied the analytic hierarchy process methodology to prioritize factors of locational preferences. Primary preference factors include secondary factors. Demand factor contains access to current and prospect markets. Service factor comprises airport and logistics perspectives. Service factor in terms of airport operations includes secondary factors such as airport service and connectivity. Service factor in terms of logistics operations contains infrastructure and logistics operations efficiency. Risk factor consists of country and business risks. We also evaluated competitiveness of Asian hub airports in terms of candidate location for regional hub distribution centers. The candidate hub airports include Singapore, Hong Kong, Shanghai, Narita and Incheon. Based on the analytic hierarchy process analysis, we derived strategic implications for hub airports to attract multinational companies’ regional hub distribution centers.

Ceramic membrane technology has been remarkably progressed for water treatment. The advantages were founded on the intrinsic properties of ceramics. Membrane fouling is regarded as a serious obstacles which deteriorate the stable purification process. The surface modification of ceramic membranes would be necessary to relieve the severe membrane fouling and to improve filtration efficiency. We aimed to develop a unique ceramic membrane with resistance to fouling. The ceramic membranes are subjected to chemical modification, and the surface charge effects were extensively investigated.

The effect of CNT diameters on properties of CNT-polyamide composites was investigated such as electrical conductivity, tensile strength and thermal conductivity. To get different diameter distributions of CNTs, several portions of Mo and Fe in Mo-Fe/MgO catalysts were synthesized by a combustion method at 600℃. And all CNTs growed at 900℃ with 3 SLM methane and 1 SLM hydrogen for 40min. Four kinds of CNTs with different diameter distributions, such as 1~3nm, 3~7nm, 7~13nm, and 10~30nm, were selected to make CNT-polyamide composites. Each composite was manufactured by a solution mixing using bar-type ultra-sonicator in the CNT portions from 1phr to 50phr. And electrical conductivity, tensile strength, and thermal conductivity were measured. Three properties of CNT-polyamide composite, manufactured with 10nm diameter, were more excellent compared to other composites, with electrical conductivity  Ω at 7phr, thermal conductivity 2.4.W/mK at 40phr, tensile strength 60MPa at 30phr. CNTs with a diameter of 10nm were superior to other diameters for the multi-functional composite such as CNT-polyamide composites.

This study was carried out to investigate the effect of malodor and VOCs reduction that could be achieved through the installation of a vapor recovery system (VRS) in a gas station. It was revealed that the reduction efficiencies of malodor by running VRS were about 93% around the oil feeder, 32% in the office and 45% in the site boundary. Specifically, it was remarkable that reduction efficiencies of BTEX over 90% were recorded through VRS operation. In addition, the results of continuous monitoring of THC around the oil feeder device provided good evidence of the inhibition of oil mist diffusion after running VRS.

The purpose of this study is to develop transgenic cell line expressing targeted human granulocyte colony stimulating factor (hGCSF) and green fluorescence protein (GFP) genes as well as production of Somatic Cell Nuclear Transfer (SCNT) embryos derived from co-expressed transgenic donor cells. Constructed pPiggy-mWAP-hGCSF-EF1-GFP vector was chemically transfected into bovine fetus cells and then, only GFP expressed cells were selected as donor cells for SCNT. Cleavage and blastocyst rates of parthenogenetic, SCNT embryos using non-TG cell and hGCSF-GFP dual expressed SCNT embryos were examined (cleavage rate: 78.0±2.8 vs. 73.1±3.2 vs. 70.4±4.3%, developmental rate: 27.2 ±3.2 vs. 21.9±3.1 vs. 17.0±2.9%). Result indicated that cleavage and blastocyst rates of TG embryos were significantly lower (P<0.05) than those of parthenogenetic and non-TG embryos, respectively. In this study, we successfully produced hGCSF-GFP dual expressed SCNT embryos and cryopreserved to produce transgenic cattle for bioreactor system purpose. Further process of our research will transfer of transgenic embryos to recipients and production of hGCSF secreting cattle.