The invasive red swamp crayfish, Procambarus clarkii, is native to southcentral United States and northeastern Mexico. Recently, it has been being spreading in the wild in South Korea. However, its primary sources, introduction routes, establishment, and expansion in South Korea remain unclear. Here, we analyzed genetic diversity and population genetic structures of its domestic natural populations during early invasion, commercial stock from local aquaria (a suspected introduction source), and original United States population using mitochondrial COI gene sequences for 267 individuals and eight microsatellite markers for 158 individuals. Natural and commercial populations of P. clarkii showed reduced genetic diversity (e.g., haplotype diversity and allelic richness). The highest genetic diversity was observed in one original source population based on both genetic markers. Despite a large number of individuals in commercial aquaria, we detected remarkably low genetic diversity and only three haplotypes among 226 individuals, suggesting an inbred population likely originating from a small founder group. Additionally, the low genetic diversity in the natural population indicates a small effective population size during early establishment of P. clarkii in South Korea. Interestingly, genetic differentiation between natural populations and the United States population was lower than that between natural populations and aquarium populations. This suggests that various genetic types from the United States likely have entered different domestic aquariums, leading to distinct natural populations through separate pathways. Results of our study will provide an insight on the level of genetic divergence and population differentiation during the initial stage of invasion of non-indigenous species into new environments.

본 연구에서는 장미 시설재배 중에 나타나는 꽃목굽음현상(BPP, bent peduncle phenomenon)의 발생 양상을 이해 하고 증상 완화 방법을 알아보고자 하였다. 실험 계획은 BPP 를 인위적으로 유도하는 고온 처리와 BPP 증상을 완화하기 위한 비정상적인 꽃받침 제거 두 가지로 설계되었다. ‘Beast’ 대상 BPP 유도실험에서 화아분화 초기 BBS2단계(bud break stage 2, 신초길이 2±0.5cm)에 30℃ 이상 고온에서 쉽게 BPP가 유도되어 고온과 BPP의 연관성을 나타냈다. 또한 6품 종(‘Propose’, ‘Beast’, ‘Revue’, ‘Ocean Song’, ‘Iguana’) 을 대상으로 엽상화 꽃받침조각을 제거한 결과 BPP 완화에 미치는 효과는 품종 특이적이었으며, 품종에 따라 상이한 결 과를 보였다. 꽃받침조각 제거 처리로 BPP가 ‘Legato’와 ‘Revue’에서 유의하게 개선되었고, ‘Beast’와 ‘Iguana’에서 는 오히려 악화되었으며, ‘Propose’와 ‘Ocean Song’에서는 증상이 정지하여 효과적이었다.

On pig farms, the highest mortality rate is observed among nursing piglets. To reduce this mortality rate, farmers need to carefully observe the piglets to prevent accidents such as being crushed and to maintain a proper body temperature. However, observing a large number of pigs individually can be challenging for farmers. Therefore, our aim was to detect the behavior of piglets and sows in real-time using deep learning models, such as YOLOv4-CSP and YOLOv7-E6E, that allow for real-time object detection. YOLOv4-CSP reduces computational cost by partitioning feature maps and utilizing Cross-stage Hierarchy to remove redundant gradient calculation. YOLOv7-E6E analyzes and controls gradient paths such that the weights of each layer learn diverse features. We detected standing, sitting, and lying behaviors in sows and lactating and starving behaviors in piglets, which indicate nursing behavior and movement to colder areas away from the group. We optimized the model parameters for the best object detection and improved reliability by acquiring data through experts. We conducted object detection for the five different behaviors. The YOLOv4-CSP model achieved an accuracy of 0.63 and mAP of 0.662, whereas the YOLOv7-E6E model showed an accuracy of 0.65 and mAP of 0.637. Therefore, based on mAP, which includes both class and localization performance, YOLOv4-CSP showed the superior performance. Such research is anticipated to be effectively utilized for the behavioral analysis of fattening pigs and in preventing piglet crushing in the future.

This study was conducted to provide basic data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS) production of a mixture of Brassica juncea extract (BJE) and fermented black rice fraction (BRF). We investigated the total phenol content, total flavonoid content, antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay) and anti-obesity activity of the mixture in 3T3-L1 cells. Our results showed that the total phenol and flavonoid content increased with increasing BRF mixture ratio. The antioxidant activity increased as the BRF mixture ratio increased. In addition, BJE and BRF mixtures did not show any cytotoxicity during the 3T3-L1 differentiation period. During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS production compared to the control group. These results warrant further experiments to develop an anti-obesity functional food using a mixture of BJE and BRF.

This study aimed to predict the shelf life of black soybean Sunsik to develop a functional labeling system for the product. The Arrhenius equation was used to calculate the shelf life by examining alterations in the dietary fiber and calcium levels of black soybean Sunsik stored at 25, 35, and 50°C for 0, 6, and 12 months. Dietary fiber and calcium analyses were performed according to the experimental methods specified in the Food Code of the Ministry of Food and Drug Safety. Both black soybean Sunsik (BS) and black soybean Sunsik containing nondigestible maltodextrin and calcium lactate (BSN) exhibited an upward trend in dietary fiber content after 12 months of storage, compared to their initial levels. During storage, the phytate in Sunsik degraded, releasing cations that facilitated the formation of new cross-links between pectic acid and middle lamella, which ultimately increased dietary fiber content. Conversely, the calcium contents of both BS and BSN decreased with prolonged storage. Based on these findings, the expected shelf life of BS and BSN was calculated as 15.65 and 28.34 months, respectively.

본 연구는 간호대학생의 그릿, 긍정적 정서, 팀워크 및 임상실습 만족도의 정도를 파악하고, 임 상실습 만족도에 영향을 주는 요인을 확인하기 위해 시행된 서술적 조사연구이다. 대상자는 U시와 G시 소 재 2개 대학 간호학과 4학년으로 하였다. 자료는 SPSS 21.0 프로그램으로 기술통계, t-test, ANOVA, Pearson 상관계수, multiple regression 분석을 하였다. 연구결과, 임상실습 만족도는 그릿, 긍정적 정서, 팀 워크와 양의 상관관계가 있었다. 팀워크는 그릿, 긍정적 정서와 양의 상관관계를 보였고, 긍정적 정서는 그 릿과 양의 상관관계를 나타냈다. 임상실습 만족도에 영향을 미치는 요인은 팀워크와 긍정적 정서였으며 설 명력은 43.6%였다. 따라서 간호대학생의 임상실습 만족도를 향상시키기 위한 전략으로 팀워크와 긍정적 정서를 향상시킬 수 있는 교육 프로그램 개발과 방안 모색이 필요하다.

본 연구는 간호대학생의 대면 및 비대면 임상실습스트레스와 스트레스 대처가 임상수행능력에 미치는 영향을 알아보고 각 요인 간 영향을 파악함으로써 간호대학생이 임상실습스트레스에 효율적으로 대처하고 임상수행능력을 증진할 수 있는 임상실습교육 전략의 근거를 제시하고자 시행되었다. 연구대상자 는 서울과 충청권 소재 간호대학생으로, 2021년 6월 10일부터 7월 10일까지 총 201부의 자료를 수집하였 다. 통계프로그램 SPSS 21.0을 이용하여 Pearson’s correlation coefficients로 대면 및 비대면 임상실습스트 레스, 스트레스 대처, 임상수행능력 간의 상관관계를, 위계적 다중회귀분석을 사용하여 임상수행능력에 미 치는 요인을 분석하였다. 연구결과, 1단계에서 간호대학생의 성적, 전공만족도, 대면실습만족도가 임상수행 능력의 19.4%를 설명하였고, 2단계에서는 스트레스 대처를 추가하여 19.6%의 설명력이 증가되어 총 39.0%의 변량을 설명할 수 있었다. 따라서 간호대학생의 임상수행능력 향상을 위해서는 임상실습스트레스 에 대한 원만한 스트레스 대처가 중요하며, 본 연구는 간호대학생의 스트레스 대처를 위한 상담 및 교육프 로그램을 개발하는 기초자료를 제공할 것이다.

Purpose: This study aimed to development and test the effects of patient safety/infection control simulation program based on a brain-based learning framework for nursing students. Methods: This pilot study used a one group pre-post test design. The study was conducted in one university in Korea. Participants were recruited using a convenience sample. Fifteen nursing students participated in this study. Results: The levels of perception of importance of patient safety management (Z=3.41, p=.001), confidence on patient safety (Z=3.30, p=.001), attitude toward personal protective equipment (Z=3.10, p=.002), and efficacy of personal protective equipment (Z=3.35, p=.001) were significantly increased. Conclusion: The application of brain-based learning framework in nursing simulation could be an effective education for nursing students.

미세플라스틱 입자는 환경 내 어디에나 존재하며 크기, 모양 또는 유형의 표준화된 입자가 아 니다. 따라서 독성자료를 바탕으로 해수, 퇴적물 등 해양환경의 다차원을 정확하게 평가하고 관리하는 위험평가 프레임워크를 구축하는 것은 매우 어렵다. 본 연구에서는 해양환경 미세플 라스틱의 특징과 영향을 조사하였고, 해양환경 미세플라스틱의 분포와 생물영향에 따른 위해 도 평가 프레임워크(초안)를 제안하고 있다. 환경 미세플라스틱의 특성은 매우 다양하지만 대 부분의 독성 데이터는 독특한 형태와 유형에 집중되어 있으며, 실험실 노출 생물과 관리해야 하는 생물 종도 다르다. 실제적으로, 지금까지의 수집된 연구결과는 위해성평가에 활용하기에 독성 데이터 품질에 있어 불확실성이 높기도 하며, 전통적인 위해성평가 프레임워크를 적용 하는 데 있어 고려할 부분이 많이 존재한다. 그러나, 현재 미세플라스틱 관리에 대한 국제사회 의 움직임이 점차 강화되고 있고, 해양환경의 미세플라스틱 오염도가 높아지고 있는 점을 고 려하면, 해양환경의 미세 플라스틱 특성에 기초한 위해성평가 기법 구축에 대한 추가 연구가 제안되어야 할 것이다.

This study aimed to investigate the protective effect of enzymatically modified stevia (EMS) on C2C12 cell-based model of dexamethasone (DEX)-induced muscle atrophy to provide baseline data for utilizing EMS in functional health products. C2C12 cells with DEX-induced muscle atrophy were treated with EMS (10, 50, and 100 μg/mL) for 24 h. C2C12 cells were treated with EMS and DEX to test their effects on cell viability and myotube formation (myotube diameter and fusion index), and analyze the expression of muscle strengthening or degrading protein markers. Schisandra chinensis Extract, a common functional ingredient, was used as a positive control. EMS did not show any cytotoxic effect at all treatment concentrations. Moreover, it exerted protective effects on C2C12 cell-based model of DEX-induced muscle atrophy at all concentrations. In addition, the positive effect of EMS on myotube formation was confirmed based on the measurement and comparison of the fusion index and myotube diameter when compared with myotubes treated with DEX alone. EMS treatment reduced the expression of muscle cell degradation-related proteins Fbx32 and MuRF1, and increased the expression of muscle strengthening and synthesis related proteins SIRT1 and p- Akt/Akt. Thus, EMS is a potential ingredient for developing functional health foods and should be further evaluated in preclinical models.

목적 : 본 연구는 신체적 기능장애인을 대상으로 장애수용, 자아존중감, 우울, 인권이 삶의 만족도에 미치 는 영향을 알아보고자 하였다. 연구방법 : 한국장애인개발원의 장애인삶 2차 패널조사에 참여한 6,121명 중 신체기능 장애인 1,780명의 데이 터를 추출하여 일반적인 특성, 장애수용, 자아존중감, 우울, 인권 항목을 분석하였다. 자료는 기술 통계, 피어슨 상관분석, 다중회귀분석을 실시하여 분석하였다. 결과 : 신체기능 장애인의 장애수용, 자아존중감, 우울, 인권과 전반적인 삶의 만족도는 유의미한 상관관계 를 보였다. 우울감이 낮을수록, 장애수용과 자아존중감, 인권이 높을수록 삶의 만족도에 긍정적인 영향을 보였다. 장애수용, 자아존중감, 우울, 인권이 전반적인 삶의 만족도에 미치는 영향은 41%의 설명력을 보 였다. 결론 : 신체적 기능장애인의 긍정적인 삶의 만족도를 위하여 장애인 자신의 긍정적 장애수용, 지역사회의 심리정서적 지원과 함께 비장애인들의 장애인식에 따른 관점변화가 이루어질 필요가 있다.

The 2017 Pohang earthquake afflicted more significant economic losses than the 2016 Gyeongju earthquake, even if these earthquakes had a similar moment magnitude. This phenomenon could be due to local site conditions that amplify ground motions. Local site effects could be estimated from methods using the horizontal-to-vertical spectral ratio, standard spectral ratio, and the generalized inversion technique. Since the generalized inversion method could estimate the site effect effectively, this study modeled the site effects in the Korean peninsula using the generalized inversion technique and the Fourier amplitude spectrum of ground motions. To validate the method, the site effects estimated for seismic stations were tested using recorded ground motions, and a ground motion prediction equation was developed without considering site effects.

Oral squamous cell carcinoma (OSCC) is the most common type of head and neck cancer and is associated with high recurrence, poor treatment, and low survival rates. Hypoxia-inducible factor-1α (HIF-1α) is a transcription factor that regulates the response to hypoxia, a major factor in the tumor microenvironment that affects tumor development and progression in various cancer types. However, microRNA (miRNA) sequence analysis revealed that only a few miRNAs targeting HIF-1α had been discovered. In the present study, we investigated HIF-1α expression in OSCC and the effect of HIF-1α-targeting miRNAs on the progression and metastatic potential of OSCC. We analyzed public databases to explore which miRNAs target HIF-1α expression. In addition, the expression of proteins involved in the cell cycle, proliferation, and apoptosis in HSC-2 cells was analyzed after miRNA-126 mimic treatment. Furthermore, to investigate the effect of miRNA-126 on the proliferation and invasion ability of OSCC cells, 5-ethynyl-2′-deoxyuridine and Transwell assays were performed. The activities of MMP-2 and MMP-9 were evaluated via gelatin zymography. Our results showed that miRNA-126, which targets HIF-1α, enhances OSCC cell proliferation by regulating the cell cycle and reinforces the cell mobility of OSCC via HIF-1α expression. These findings suggest that miRNA-126 may be a novel marker for OSCC treatment and the development of new tools for patients with OSCC.

본 연구에서는 칡의 효율적인 제거 방법을 모색하기 위해 국내외의 사례를 비교하였다. 칡은 척박한 토지에서도 생장이 왕성한 특성을 가져 사면침식을 억제하는 식물로 이용되기도 하였다. 그러나 칡은 덩굴을 형성하여 조림목을 감고 올라가 생장하므로 심할 경우에는 조림목의 고사를 유발한다. 최근에는 생활권 수목에 대한 관심의 증가로 조경수에 대한 피해가 주목을 받고 있다. 칡은 물리적, 화학적 및 생물학적 방법으로 제거한다. 국내에서는 덩굴 및 주두부 제거 등 물리적 제거 방법과 제초제를 사용하는 화학적 방법을 주로 사용하고 국외에서는 화학적 방법에 주로 의존한다. 물리적 방법은 환경오염이 적은 이점이 있으나 상당한 노동력과 비용이 소요된다. 화학적 방법은 즉각적으로 제거 효과가 나타나지만 약제 사용에 의한 환경오염과 기상조건의 제약이 있다. 생물학적 방법은 화학적 방법의 환경오염을 해결하기 위해 개발되었으나 제거 효과가 느리게 나타나고 조림지에 적용 시 조림목과 하층식생에 미치는 영향에 대한 우려가 있다. 향후 고효율성을 담보하며 친환경적인 생력화 칡 제거 기술에 대한 연구와 적용이 필요하다.

This study was conducted to explore the change in the market issues on HMR (Home Meal Replacements) using local foods after the COVID-19 outbreak. Online text data were collected from internet news, social media posts, and web documents before (from January 2016 to December 2019) and after (from January 2020 to November 2022) the COVID- 19 outbreak. TF-IDF analysis showed that ‘Trend’, ‘Market’, ‘Consumption’, and ‘Food service industry’ were the major keywords before the COVID-19 outbreak, whereas ‘Wanju-gun’, ‘Distribution’, ‘Development’, and ‘Meal-kit’ were main keywords after the COVID-19 outbreak. The results of topic modeling analysis and categorization showed that after the COVID-19 outbreak, the ‘Market’ category included ‘Non-face-to-face market’ instead of ‘Event,’ and ‘Delivery’ instead of ‘Distribution’. In the ‘Product’ category, ‘Marketing’ was included instead of ‘Trend’. Additionally, in the ‘Support’ category, ‘Start-up’ and ‘School food service’ appeared as new topics after the COVID-19 outbreak. In conclusion, this study showed that meaningful change had occurred in market issues on HMR using local foods after the COVID-19 outbreak. Therefore, governments should take advantage of such market opportunity by implementing policy and programs to promote the development and marketing of HMR using local foods.