This study was carried out to investigate milling's effect on the pasting properties and storage stability of dry-milled rice flour. Rice flour's moisture content was increased from 9.48% to 9.80% after going through a rice polisher, and the crude fat content of rice flour was decreased from 0.91% to 0.62% after going through a rice polisher. In the color index of rice flour, the rice polisher was only affected by yellowness. The pasting properties were verified through RVA, and it was confirmed that the use of a rice polisher had no significant effect on the pasting properties. As a result of observing the changes in fatty acid value, it was ascertained that the storage period could be increased using the rice polisher. These results suggest that the rice polisher can increase the storage period without changing the pasting properties.

Glial cells, including astrocytes and microglia, interact closely with neurons and modulate pain transmission, particularly under pathological conditions. In this study, we examined the excitability of substantia gelatinosa (SG) neurons of the spinal dorsal horn using a patch clamp recording to investigate the roles of microglial activation in the nociceptive processes of rats. We used xanthine/xanthine oxidase (X/XO), a generator of superoxide anion (O2∙–), to induce a pathological pain condition. X/XO treatment induced an inward current and membrane depolarization. The inward current was significantly inhibited by minocycline, a microglial inhibitor, and fluorocitrate, an astrocyte inhibitor. To examine whether toll-like receptor 4 (TLR4) in microglia was involved in the inward current, we used lipopolysaccharide (LPS), a highly specific TLR4 agonist. The LPS induced inward current, which was decreased by pretreatment with Tak-242, a TLR4-specific inhibitor, and phenyl N-t-butylnitrone, a reactive oxygen species scavenger. The X/XO-induced inward current was also inhibited by pretreatment with Tak-242. These results indicate that the X/XO-induced inward current of SG neurons occurs through activation of TLR4 in microglial cells, suggesting that neuroglial cells modulate the nociceptive process through central sensitization.

The purpose of this study was to derive the conditions for manufacturing rice porridge with optimum properties after reheating. The characteristics of rice porridge according to the soaking time, water addition rate, heating temperature, heating time, and cooling conditions were compared using the ‘Samkwang’ cultivar. In Step Ⅰ, as the heating temperature increased, the weight change decreased and the viscosity increased, and the temperature known as the main factor of the gelatinization also appeared to affect the viscosity increase. In Step Ⅱ, the viscosity and the texture properties was not significantly different as the soaking time was reduced, and 10 minutes was suitable because of due to the shortening effect of the total process time. In Step Ⅲ, the residual heat was lowered by cooling after the rice porridge production, so the viscosity could be greatly reduced. Also, it was confirmed that the water addition rate of 900% and the heating temperature of 15 minutes were optimal manufacturing conditions. The next study will investigate the porridge processability of rice cultivars using these results.

When producing rice products, it is very important to select suitable raw materials. Therefore, in this study, the quality characteristics of 16 rice cultivars were quantified to determine the criteria for evaluating the machinability of raw rice. The L value, which can affect the color of porridge prepared from rice, was the greatest for Hwaseonchalbyeo (84.17). The water-binding capacity, related to water interaction, was high in Hyangcheola (113.2%), and water solubility was high in Shingil (22.3%). Dodamssal (42.3%, 70.7 RVU) and Hwaseonchalbyeo (4.7%, 27.8 RVU) showed lower final viscosity compared to the cultivars in which the amylose content was medium groups (16.4~21.2%, 173.6~277.2 RVU). Specifically, cultivars with high or low amylose content had a low viscosity. The characteristics of the distribution of raw rice quality data were confirmed through 11 histograms. Furthermore, amylose content vs. water solubility, water solubility vs. peak viscosity, and peak viscosity vs. final viscosity showed high correlations (r=0.542, －0.569, and 0.836 respectively, p<0.01), and clear cultivar discrimination by the standard error of the mean (0.765~10.811). In conclusion, amylose content, water solubility, and peak viscosity were considered the most suitable characteristics for the quality evaluation of raw rice.

In this study, the physicochemical traits of soybean (100-seed weight, seed coat rate, protein content, composition, and amino acid content) and the quality of tofu (texture and sensory characteristics) were evaluated in order to determine the features of six varieties (Saedanbaek, Daechan, Daepung2, Seonpung, Miso, Saegeum) in terms of textural and sensory characteristics of tofu. Regardless of cultivar, the order of amino acid composition rate was as follows: Glutamic acid > Aspartic acid > Arginine > Leucine > Lysine. Approximately 50-60% of tofu (dry weight) consisted of protein, and among the cultivars, Tofu made from Saedanbaek showed the highest amount of protein at 59%. It was followed by tofu made from Miso at 54%. The rest contained about 50% protein. According to the result of correlation, crude lipid (-0.933**), crude protein (0.961**), and total phosphorus (0.924**) were strongly correlated with tofu hardness, and such factors could be utilized as an indicator of tofu quality.

This study attempts to analyze the physicochemical characteristics of rice flour developed as rice powder for exclusive use, steamed rice cake recipes developed as basic recipefor rice cakes. The study also aims to compare the quality characteristics of steamed rice cakes in an effort to establish the possibilities of developing various rice cake recipes. The moisture content and amylose content increased as the rice powder particles for exclusive use became finer. With regards to water uptake, in the case of the Hungara type, the water uptake increased as rice powder particles increased in size. In the case of the Shingil and commercial types, the water uptake decreased as rice powder particles increased in size, and solubility and swelling force decreased as rice powder particles increased in size. In the case of chromaticity, Sulgidduk made with rice powder for exclusive use the brightness (L value) increased as powder particles became finer and the yellow indexes (b value) decreased, and this was especially pronounced in the Shingil type. The measurements of changes in the mechanical textures of Sulgidduk showed that rice types with larger particles led to lower hardness, cohesiveness, springiness, and chewiness.

In this study, the product characteristics and physicochemical properties were investigated through collection of commercial porridge. The addition rate of grain raw materials was about 6.5~11.75%, glutinous rice was added at a rate of about 23~60% to improve the viscosity and various other food additives were used. The moisture content characteristics varied among the products. The rotational viscosity of CP (Commercial Porridge)4 was the highest at 39,054 cP, while the flow viscosity of CP3 was least at 4.80 cm/30 seconds. The starch content differed among the products in the range of total starch 6.96~8.08%, amylose 1.41~2.61%, total sugar 6.55~12.81% and reducing sugar 0.50~0.99%. Particularly, total sugar showed a very high correlation ( 0.920) while rotational viscosity and color value (b) showed significant correlation with most of the properties i.e. moisture, solids content etc. There was a rapid increase in the reactivity of starch degrading enzyme at the early stage of the reaction which gradually decreased with time. The physicochemical characteristics of commercial porridge presented in this study could be expected to increase the industrial use value of the related research because it considers the quality of the currently commercialized porridge for the future selection of suitable porridge raw materials.

Reactive oxygen species (ROS) and nitrogen species (RNS) are involved in cellular signaling processes as a cause of oxidative stress. According to recent studies, ROS and RNS are important signaling molecules involved in pain transmission through spinal mechanisms. In this study, a patch clamp recording was used in spinal slices of rats to investigate the action mechanisms of O2 ⦁- and NO on the excitability of substantia gelatinosa (SG) neuron. The application of xanthine and xanthine oxidase (X/XO) compound, a ROS donor, induced inward currents and increased the frequency of spontaneous excitatory postsynaptic currents (sEPSC) in slice preparation. The application of S-nitroso-N-acetyl-DLpenicillamine (SNAP), a RNS donor, also induced inward currents and increased the frequency of sEPSC. In a single cell preparation, X/XO and SNAP had no effect on the inward currents, revealing the involvement of presynaptic action. X/XO and SNAP induced a membrane depolarization in current clamp conditions which was significantly decreased by the addition of thapsigargin to an external calcium free solution for blocking synaptic transmission. Furthermore, X/XO and SNAP increased the frequency of action potentials evoked by depolarizing current pulses, suggesting the involvement of postsynaptic action. According to these results, it was estblished that elevated ROS and RNS in the spinal cord can sensitize the dorsal horn neurons via pre- and postsynaptic mechanisms. Therefore, ROS and RNS play similar roles in the regulation of the membrane excitability of SG neurons.

1. 주요 쌀 생산국 브랜드 쌀 및 MMA 쌀 40점의 품질을 조사한 결과, 수분함량은 11.6(인도)~14.2%(중국), 단백질 함량은 5.82(미국)~7.86%(중국), 아밀로스 함량은 16.2(태국)~24.4%(인도), 윤기치는 45.2(인도)~75.7(미국)의 범위를 나타내었다. 2. 국가별 수집된 브랜드 쌀의 형태적 특성을 살펴본 결과, 중국과 베트남의 브랜드 쌀은 주로 통통한 단립종이었고, 미국 브랜드 쌀은 중간 두께의 중립종이었으며, 태국 브랜드 쌀은 얇은 장립종의 형태를 나타내었다. 호주와 인도는 단립, 중립, 장립의 다양한 형태의 쌀을 확인 할 수 있었다. 3. 국가별 브랜드 쌀의 백미 품위를 살펴본 결과 완전미율은 72.4(인도)~95.2(태국)%의 범위였으며, 베트남 브랜드 쌀은 동할미 비율이 높았으며, 인도 브랜드 쌀은 분상질미 비율이 높은 특징을 나타내었다. 4. RVA를 이용한 호화특성을 분석 한 결과, 인도의 브랜드 쌀이 최고온도가 낮고 치반점도가 높으며 강하점도가 낮아 취반미의 찰기가 낮을 것으로 예측되었으며, 호화개시온도 또한 유의하게 높은 것을 확인할 수 있었다. 5. 국가별 브랜드 쌀의 식미 관능평가를 실시한 결과, 수집된 브랜드 쌀의 평균 종합기호도는 미국(-0.59), 중국(-0.76), 호주(-1.23), 베트남(-1.43), 태국(-2.09), 인도(-2.49) 순으로 나타났다.

Variations among 15 major waxy cultivars in terms of milling appearance and cooking characteristics at different moisture contents were investigated. Hardness of milled rice kernels increased and 1000-grain weights decreased with the reduction of moisture content. The milled rice kernels showed about two-fold hardness gap between 12 and 16% moisture contents. The 1000-grain weights revealed 6% reduction from 21.4 g to 20.2 g at 16% and 12% moisture contents, respectively. The whiteness of waxy rice which indicated varietal variation also increased with decrease of moisture contents. In most cultivars, the whiteness of waxy rice peaked and stabilized at around 14% moisture content. In milling properties, brown/rough rice ratios were not affected by moisture content, however, milled/brown rice ratios increased with the decrease of moisture contents between 14 - 16%. This implied that the proper moisture content for milling rate of waxy rice is lower than that of non-waxy rice. The chalkiness expression of milled waxy rice varied directly with moisture contents. At 16% moisture content, the rates of translucent and semi-translucent kernels like non-waxy rice were 49.0 - 84.4% while at 14% moisture content, the rates of chalky rice were 88.7 - 99.9%. In terms of cooking properties of milled waxy rice with different moisture contents, lower moisture contents (12 - 13%) were related with higher water absorption rates. The higher volume expansion of cooked milled rice and more soluble solid after cooking in most cultivars showed the possibility of low palatability of cooked waxy rice with lower moisture contents (below 13%).

Nitric Oxide (NO) is an important signaling molecule in the nociceptive process. Our previous study suggested that high concentrations of sodium nitroprusside (SNP), a NO donor, induce a membrane hyperpolarization and outward current through large conductances calcium-activated potassium (BKca) channels in substantia gelatinosa (SG) neurons. In this study, patch clamp recording in spinal slices was used to investigate the sources of Ca²+ that induces Ca²+-activated potassium currents. Application of SNP induced a membrane hyperpolarization, which was significantly inhibited by hemoglobin and 2-(4-carboxyphenyl) -4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide potassium salt (c-PTIO), NO scavengers. SNP-induced hyperpolarization was decreased in the presence of charybdotoxin, a selective BKCa channel blocker. In addition, SNP-induced response was significantly blocked by pretreatment of thapsigargin which can remove Ca²+ in endoplasmic reticulum, and decreased by pretreatment of dentrolene, a ryanodine receptors (RyR) blocker. These data suggested that NO induces a membrane hyperpolarization through BKca channels, which are activated by intracellular Ca²+ increase via activation of RyR of Ca²+ stores.

본 시험은 열대지역적응 자포니카인 진미벼, IRKR 1 (IRRIRice and Korea Rice 1 약칭, 계통명은 IR 68333-R-R-B-22)및 인디카인 IR 64 품종에 대하여 필리핀인을 주요 대상으로쌀 외관 및 밥맛 선호도를 평가하여 개발된 품종의 농가 재배확대 및 새로운 품종개발의 기초자료로 이용하고자 수행하였다. 1. 쌀의 외관품질 선호도 평가에서 패널로 참여한 필리핀인은 투명도·외관 > 향·색깔 > 쌀의 길이·모양 순이었다. 한국인은 쌀의 길이·모양· 투명도 > 외관 > 색깔·향 순이었다. 따라서 필리핀인은 완전미율이 높고 향이 나는 쌀을, 한국인은 단원립이면서 완전미율이 높은 쌀을 선호하였다. 2. 식미관능평가 결과 필리핀인은 인디카 고아밀로스 품종인 IR 64보다 자포니카 중간아밀로스인 진미벼와 IRKR 1을선호하여 기존에 알려진 인디카를 선호한다는 선행 연구결과와는 상이하였다. 3. 따라서 패널로 참여한 필리핀인의 자포니카 쌀에 대한 선호도를 보다 정확하게 평가하기 위해 식미관능평가 5개 항목별 선호도를 분석한 결과, 밥의 모양, 냄새, 맛, 질감은 통계적차이가 없었다. 반면 찰기는 진미벼 보다는 IRKR 1을 선호하였다. 반면 한국인은 진미벼 보다 IRKR 1이 밥의 찰기가 적고 질감이 거칠면서 딱딱한 촉감으로 인해 진미벼를 선호한것으로 고찰된다. 4. 이상의 결과를 요약하면, 패널로 참여한 필리핀인이 선호하는 자포니카 쌀의 식미는 한국인이 선호하는 것 보다는 찰기가 적고, 질감은 좀 더 딱딱한 것으로 사료된다.

밥맛은 밥쌀용 쌀 품종의 품질을 평가하는 중요한 항목 중 하나로, 훈련된 패널을 활용하여 평가하고 있다. 본 연구는 밥맛 평가의 정밀도 향상을 위하여 전자혀의 일종인 taste sensing system을 이용하여 관능적 식미 특성이 다른 쌀 10 품종의 밥맛을 측정하여 분석하였다. 분석 결과, taste sensor를 이용한 밥맛 측정은 밥의 쓴맛, 감칠맛, 단맛 측정에 적합한 것으로 나타났고 감칠맛의 경우, 관능적 평가에서 밥맛이 좋게 평가되었던 삼광의 측정값이 가장 높았고, 관능적 평가에서 밥맛이 나쁘게 평가되었던 한아름은 가장 낮은 측정값을 보였으며 품종간의 값 차이는 유의성이 인정되었다. 이러한 결과는 taste sensor를 밥맛 측정에 활용할 수 있다는 가능성을 보여주며, taste sensing system을 본격적으로 밥맛 측정에 활용하기 위해서는 다양한 조합을 통한 상관 분석이 필요할 것으로 생각된다.

국내 벼 품종 중 아밀로스 함량과 아밀로펙틴 조성이 다양한 품종의 막걸리 특성을 분석하여 막걸리 품질 고급화 및 다양화를 위한 자료를 제공하고자 본 실험을 수행한 결과는 다음과 같았다. ‘고아미2호’, ‘고아미3호’, ‘고아미4호’는 품종별 전분 특성과 호화 특성 분석 결과, 낮은 아밀로펙틴 단쇄 비율과 높은 아밀로스 함량에 기인하여 점성이 낮게 나타남을 확인할 수 있었고, 높은 호화개시온도로 인해 가열흡수비와 팽창용적이 각각 2.09~2.13, 25.02~26.78 ㎤로 다른 품종에 비해 유의하게 낮아, 막걸리 발효에 적합한 전분의 호화가 이루어지지 않음을 확인할 수 있어, 막걸리 제조를 위해 호화를 증진시키기 위한 가공 방법 연구가 필요할 것으로 판단되었다. 품종별 막걸리의 품질 특성은 ‘설갱’, ‘백진주’, ‘일품’의 알코올 함량은 17.3~18.0% 범위로 통계적인 유의차는 없었으나, ‘고아미2호’와 ‘고아미3호’, ‘고아미4호’는 10.0~10.5%로 상대적으로 낮은 알코올 함량을 나타내었고, 당도 또한 ‘설갱’, ‘백진주’, ‘일품’에 비해 절반 가까이 낮았으나, pH와 산도는 품종 간 유의한 차이를 나타내지 않았다. 또한 원료곡에서 높은 난소화성 다당류 함량을 지닌 ‘고아미4호’ 등은 막걸리에서도 역시 난소화성 다당류 함량이 유의하게 높아, 향후 막걸리 제품으로 이용 시 건강 기능성에 기여할 가능성이 높을 것으로 기대되었다. 결과적으로 아밀로펙틴 조성과 난소화성 다당류 함량에서 차이를 보인 ‘고아미2호’, ‘고아미3호’, ‘고아미4호’ 막걸리의 높은 제조수율과 난소화성 다당류 함량 및 낮은 알코올 함량과 당도를 확인하였으며, 지속적인 발효 공정 연구를 통해 국내 기능성 품종을 이용한 고품질 막걸리 개발이 필요할 것으로 판단된다.

본 연구에서는 몇 가지 벼 품종(삼광, 미광, 칠보, 청해진미 및 조운)의 도정 부산물인 미강에 대한 추출 온도에 따른 70% 에탄올 추출물의 항산화 성분(polyphenol, flavonoid)과 항산화 활성(DPPH radical 소거능, ABTS radical 소거능, 환원력)을 비교?분석하여 기능적 가치를 평가함으로써 이용 가능성을 살펴보고자 하였다. 추출 온도에 따른 미강의 항산화 성분을 측정한 결과, 총 polyphenol 함량에서는 추출 온도가 높을수록 다소 증가하는 경향을 나타낸 반면, 총 flavonoid 함량은 추출 온도에 따른 차이는 보이지 않았다. 항산화 활성측정 결과, DPPH radical 소거능을 제외한 총 항산화력(ABTS radical 소거능)과 환원력은 온도가 높아짐에 따라 유의적으로 증가하는 경향을 나타내었다. 또한 조사되어진 품종들의 미강 최적 추출조건은 청해진미로 25℃에서 추출하였을 경우에 가장 우수한 항산화 효과를 얻을 수가 있었다. 이에 본 연구결과를 토대로 최적의 기능성 성분을 활용할 수 있는 추출 온도를 제공함으로써 도정 부산물의 최적 추출조건 및 품종별 쌀의 기초 자료를 제공할 수 있을 것이라 사료된다.

Paddy rice is typically stored during postharvest until rice grain is processed into brown rice and milled rice by hulling and milling procedure, respectively. Recently, instead of storing paddy rice, storage of brown rice has been in the spotlight because it is more convenient and economically feasible. Different brown rice cultivars with varying amylose contents including waxy rice, medium-waxy rice, and non-glutinous rice were stored in room temperature storage for four months, and the changes in grain qualities of brown rice were evaluated. Amylose content significantly affected pasting properties in which rice cultivar with higher amylose content showed longer pasting time and higher peak viscosity. Storage also affected pasting viscosities, showing an increase in peak viscosity, but a decrease in breakdown viscosity. The changes in pasting viscosity during storage could be an important starch property for aged brown rice utilization. Waxy brown rice showed the weakest aging property in terms of free fatty acids (FFA) accumulation, whereas non-glutinous rice was more substantial grain quality against aging. The FFA values of two months storage were not significantly different from the initial FFA contents, suggesting that brown rice stored in room temperature for two months could be feasible for direct consumption of brown rice.

In this study, we analyzed seed storage proteins in order to investigate the main factors related to the eating quality of japonica and tongil-type rice varieties. Sensory evaluation was performed by a trained panel to assess the appearance (color and glossiness), flavor, taste, stickiness, texture, and overall score of nine japonica and three tongil-type rice cultivars. Moreover, the pattern of variation in rice storage proteins was examined by electrophoresis of protein extracts. The electrophoretic pattern of rice proteins showed 16.4 kDa albumin, 26.4 kDa globulin, 34-39 kDa and 21-22 kDa glutelin, and 14.3 kDa prolamin. In terms of storage protein, the varietal differences between japonica and tongil-type rice were found in albumin, globulin, and the α-1, and α-2 sub-units of acidic glutelin. Furthermore, the overall sensory evaluation score was observed to be positively correlated with albumin (0.495 ** ) and globulin (0.567 ** ), and negatively correlated with α-1 glutelin (-0.612 ** ). Therefore, the results indicated that albumin, globulin, and α-1 glutelin can affect the eating quality of japonica and tongil-type rice varieties, with the latter having lower eating quality than the former.

조숙 내병 고품질 찰벼를 육성할 목적으로 2000년 하계에 조숙 내도열병 내도복성인 SR20261-5-4-10 계통을 모본으로 조생찰벼인 진부찰을 인공교배하여 SR26688-1-2-2 계통을 선발하여 철원77호로 계통명을 부여하였다. 2008～2010년 3 개년 간 지역적응시험 실시결과 그 우수성이 인정되어 2010 년 12월 직무육성 신품종 선정위원회에서 국가목록등재 품종 으로 선정됨과 동시에 ‘청백찰’로 명명되었다. ‘청백찰’의 출 수기는 보통기 재배에서 평균 출수기가 7월27일로 ‘오대’보 다 4일 빠른 극조생종이다. 벼키는 68 cm로 ‘오대’의 69 cm 와 비슷하며, 이삭길이와 주당 이삭수도 19 cm와 14개로 ‘오 대’와 비슷하나 이삭당 입수는 80개로 ‘오대’보다 많으며, 등 숙율은 78.5%로 ‘오대’보다 약간 낮고, 현미 천립중이 22.3 g으로 중립종이다. ‘청백찰’은 도열병저항성은 ‘오대’보다 강 하나, 흰잎마름병, 줄무늬잎마름병 및 벼멸구에 대한 저항성 이 없다. ‘청백찰’은 불시출수 현상은 ‘오대’와 비슷하였고 위 조현상은 없고, 내랭성검정 결과 내랭성이 ‘오대’와 비슷한 정도를 보였으며, 도복에 강한 편이다. ‘청백찰’의 제현율은 80.8%로 ‘오대’(82.5%) 보다 약간 낮고, 현미 장폭비가 1.86 으로 중단원립이다. 백미외관은 찹쌀이므로 뽀얗게 보이고, 알칼리 붕괴도는 ‘오대’의 6.7에 비해 5.5로 낮으며 단백질함 량은 7.1%로 ‘오대’의 6.7보다 약간 높다. 북부평야, 중산간 지 및 남부 고랭지 5개소, 동북부 해안 1개소, 중북부 고랭지 1개소에서 보통기 보비재배 평균 쌀수량은 5.35 MT/ha로 대 비품종 ‘오대’의 5.39 MT/ha과 비슷한 수량성을 보였다. ‘청 백찰’의 재배적지는 북부평야, 중산간지, 남부 및 중북부 고랭 지 및 동북부 해안지이다.

The rice bran, a by-product of rice milling process, is well known for various functional components, such as tocopherol, tocotrienol, γ-oryzanol, carrying antioxidant activities. This study was conducted to investigate the antioxidant components and antioxidant activities in rice bran of different Korean rice cultivars. The 8 isomers of vitamin E, γ-oryzanol, flavonoids, and polyphenolics in rice bran from 16 Korean premium and high quality rice cultivars were quantified. DPPH and ABTS radical scavenging activities and reducing power of the ethanol extracts of rice bran were measured. ‘Hopum’ showed the highest total vitamin E content, 221.47 μg/g among the cultivars, and ‘Hanseol’ showed the lowest content. The rice bran showed different compositions of α-, β-, γ-, δ- tocopherol and tocotrienol among rice cultivars. The antioxidant contents were also different by cultivar; the γ-oryzanol contents ranged from 1.99 mg/g (Unkwang) to 4.30 mg/g (Chilbo), the polyphenol contents ranged from 427.22 mg gallic acid eq./100 g (Odaebyeo) to 775.80 mg gallic acid eq./100 g (Hopum). ‘Hopum’ also had the highest DPPH and ABTS free radical scavenging activities, 9.82% and 187.5 AEAC mg/100 g, respectively. In vitro, the rice bran extracts from ‘Hopum’ had significantly higher antioxidant activities than that of other cultivars.