Pellicle is defined as a thin transparent film stretched over an aluminum (Al) frame that is glued on one side of a photomask. As semiconductor devices are pursuing higher levels of integration and higher resolution patterns, the cleaning of the Al flame surface is becoming a critical step because the contaminants on the Al flame can cause lithography exposure defects on the wafers. In order to remove these contaminants from the Al frame, a highly concentrated nitric acid (HNO3) solution is used. However, it is difficult to fully remove them, which results in an increase in the Al surface roughness. In this paper, the pellicle frame cleaning is investigated using various cleaning solutions. When the mixture of sulfuric acid (H2SO4), hydrofluoric acid (HF), hydrogen peroxide (H2O2), and deionized water with ultrasonic is used, a high cleaning efficiency is achieved without HNO3. Thus, this cleaning process is suitable for Al frame cleaning and it can also reduce the use of chemicals.

군위지역의 돈사를 중심으로 농업시설의 비활용 공간인 지붕을 활용하기 위한 기초자료로써 지붕의 방위각, 경사각 및 농지의 분류에 따른 문제점을 분석하였다. 또한 비표준형 돈사의 구조해석과 보강방안에 대한 대책을 강구하였고, 태양에너지 생산을 위한 지붕의 방위각과 위도에 따른 일사량을 측정한 결과는 다음과 같다. 돈사의 농지의 분류에 따른 결과는 군위지역 돈사의 경우 농림지역을 제외한 곳은 47.8%이었으며 적정 방위각으로 분류하면 21.7%가 활용 가능하였다. 그러나 농지와 방위각을 모두 고려한 조사에서는 10.9%만이 가능하여 돈사의 약 90%는 지붕의 활용이 부적합 한 것으로 판단되었다. 돈사의 지붕 경사각에서는 총 46호 중 10°∼15°가 17호로 가장 많았으며, 20°미만인 경우가 전체의 63%에 해당되었다. 20°이상인 곳은 16호로 전체의 약 35%로 나타나 전반적으로 지붕각은 완만하게 건축되었다. 따라서 돈사의 경우 일사량을 고려하여 태양에너지 설비를 할 경우 최소한 약 4∼5° 정도 지붕경사각이 낮음을 알 수 있었다. 비표준형 돈사에 대한 구조해석을 통하여 보강방안에 대한 대책을 강구하였다. 지붕의 방위각과 위도에 따른 일사량의 측정에서는 정남방향을 기준으로 위도가 27.5°∼50°까지 비교적 높게 나타났으며, 위도를 0°로 고정하였을 경우, 방위각은 S22.5W와 S22.5E에서 높은 것을 알 수 있었다. 따라서 적정 지붕의 경사각은 25°이상으로 건축하는 것이 유리한 것으로 판단되었다.

우리나라의 경남지역에서 장류콩의 적정파종시기를 구명하고자 본 시험에서 중간생육,성숙기 생육, 수량성, 포장병발생정도 및 성분분석을 비교한 결과는 다음과 같다. 1. 잎의 전개 및 생식생장기의 주요생육단계의 도달에 소요되는 기간은 두 품종 모두 파종기가 빠를수록 길어졌다 2. R5 stage에 있어서 선유콩은 6월 10일 파종에서 단위면적당 건물중이 가장 많았고 전 파종시기에서 최적엽면적지수의 범위에 포함되었으며, 대원콩은 파종시기가 빠를수록 건물중이 많았고 엽면적지수가 6월 25일 파종을 제외하고는 최적엽면적지수 에 미쳤다. Sink/Source이 비율은 선유콩과 대원콩 모두 5월 25일 파종에서 가장 낮아 엽이 부담하는 협의 비율이 가장 적었다. 3. 선유콩의 수량성은 파종기간 유의한 차이는 없었지만 5월 25일 파종에서 가장 많았고, 대원콩의 수량성은 수량은 5월 25일 파종에서 유의하게 가장 많았고 나머지 파종기간에는 비슷하였는데 6월 10일 파종에서 ‘09년 검은 뿌리썩음병의 많은 발생과 3년간 불마름의 발생이 수량성 저하에 크게 영향을 미쳤다. 4. 파종시기 간에 단백질과 지방의 함량은 차이가 없었고, 지방산에 있어서 Oleic acid는 파종기가 빠를수록 그리고 Linolenic acid 는 파종기를 늦을수록 미세하게 증가하였으며 아이소플라본 함량은 파종시기가 늦어질수록 높았다. 5. 경남지역에서 이상의 건물중 및 엽면적지수, 100립중, 도복종실 열피립의 피해 감소 및 수량성을 고려 할 때 장류콩(선유콩 및 대원콩)의 적정파종시기는 5월 하순 ~ 6월 상순이었다.

우리나라의 남부지역에서 콩의 비닐피복 재배시에 적정파종시기를 구명하고자 본 시험에서 주요 특성 및 수량성을 비교한 결과는 다음과 같다. 1. 비닐피복재배에서 파종기가 빠를수록 그리고 영양생장기 중 V6 stage 까지는 생육일수가 많이 단축되었으나 그 이후는 단축효과는 점차로 적었다. 2. 콩의 개화에 필요한 평균소요일수는 노지재배에서 컸고 성숙에 필요한 평균소요일수는 비닐피복 재배에서 컸다. 3. 콩의 비닐피복 재배시 R5 stage의 건물중은 노지재배보다 무겁고 엽면적지수도 최적엽면적지수인 5~7의 범위에 포함되었다. 4. 비닐피복 재배시 5월 25일 및 6월 10일 파종에서 콩의 립수가 많고 백립중이 무거워 수량이 높았다 5. 단백질함량은 비닐피복 재배에서 낮고 파종기간 차이가 없었으며 지방함량은 재배양식 및 파종기간 비슷하였다. 한편비닐피복 재배시 지방산 C18:1의 함량은 5월25일 및 6월 10일 파종에서 많았고 C18:3은 재배양식 및 파종기간 차이가 없었다. 6. 아이소플라본 함량은 비닐피복 재배에서 그리고 파종기가 늦을수록 많았다.

The mixing of rice and brown rice produced in different years is banned in Korea by the grain management act. However, there has been no reported method for discriminating the production year of rice. The objective of this study was to develop a method for discriminating the production year of rice and brown rice based on their phospholipids content. One hundred rice samples and 130 brown rice samples produced between 2012 and 2015 were collected. Twelve phosphatidylcholine components were analyzed by liquid chromatography-tandem mass spectrometry. Phosphatidylcholine was used as an internal standard to calculate the peak intensity of the samples. A statistical analysis of the results showed that the centroid distance between the stale and new rice was 4.16 and the classification ratio was 97%. To verify the calculated discriminant, 61 and 40 rice samples were collected. The accuracy of discrimination was 82% by primary verification and 80% by secondary verification. The statistical analysis of brown rice showed that the centroid distance between the stale and new brown rice was 3.14 and the classification ratio was 96%. To verify the calculated discriminant, 10 samples of new rice and 30 samples of stale rice were collected and the accuracy of discrimination was 93%. The accuracy of discrimination for rice stored at room temperature was 57.9 –92.1% and that for rice stored at a low temperature was 86.8–94.7%, depending on the storage period. For brown rice, the detection accuracy was 94.7–100% at room temperature and 92.1–100% at a low temperature, depending on the storage period. The accuracy of discrimination for rice was affected by the storage temperature and time, while that for brown rice was more than 92% regardless of the storage conditions. These results suggest that the developed discriminant analysis method could be utilized to determine the production year of rice and brown rice.

A mungbean cultivar ‘Sanpo’ (Vigna radiata (L.) Wilczek) was derived from the cross between SM9710-3B-7 and Jangannogdu at the Jeollanamdo Agricultural Research and Extension Services (JARES) in 2012. ‘Sanpo’ has erect growth habit, green hypocotyls, heart leaflet, dull green seed surface, and black and straight pod when matured. The stem length of ‘Sanpo’ were 71㎝, which was 5㎝ longer than check cultivar ‘Owool’. The pods number per plant, the seeds number per pod, and the seed weight of ‘Sanpo’ are similar to check cultivar. It has field resistance to cercospora leaf spot, mungbean mottle virus, and powdery mildew. In addition, ‘Sanpo’ has high lodging resistance. The hard seed rate of ‘Sanpo’ was 2.4%, which was 0.8%point lower than check cultivar. The sprout yield ratio of ‘Sanpo’ was 8.26 times, which was 10% (77%point) more than check cultivar. The average yield of ‘Sanpo’ was 1.62ton/㏊, which was 14% more than the check cultivar ‘Owool’. (Cultivar registration number: 5250)

A new soybean variety, ‘Joongmo 3009’ (Milyang 222) was developed at the National Institute of Crop Science (NICS) in 2012. ‘Joongmo 3009’ was released by pedigree selection from the cross between ‘Cheongja 2(Milyang 121)’ and ‘Daemangkong’. It has determinate growth habit, white flower, brown pubescence, brown pod color, green seed coat, green cotyledon, spherical seed shape, oval leaf shape and large seed size (29.3 grams per 100 seeds). It was late 16 days in maturing date than the check cultivar ‘Cheongjakong’. The average yield of ‘Joongmo 3009’ was 2.91 ton per hectare, which was higher 36 percentage than the check variety, in the regional yield trials carried out in three adaptable locations of Korea from 2010 to 2012. The number of breeder’s right is ‘5474’

Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and 25°C). However, whole length, hypocotyl length, and sprout yield were highly increased at 23°C compared to 20 and 25°C. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at 23°C than at 20 and 25°C, while antioxidant properties of PI343278 and PI343283 were decreased in a temperaturedependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts.

본 연구는 장류용 콩 품종의 논 재배 시 만파에 따른 생 육 및 수량성 변이를 검토하기 위하여 경남 밀양에서 2012 년부터 2013년에 걸쳐 수행하였다. 이용된 품종은 대원콩, 남풍, 대풍, 새단백, 선유, 우람, 천상, 태광 등의 중만생종 품종과 참올, 황금올의 조생종 품종이었으며 6월 20일, 7월 5일, 7월 20일 세 차례에 걸쳐 파종하였다. 연구결과를 요 약하면 다음과 같다. 1. 경장은 중만생종에서 만파할수록 대체로 감소하였으며 조생종에서는 유의한 변화가 관찰되지 않았다. 7월 20 일 파종 시 분지수는 태광, 대풍, 선유, 참올 등에서 많 이 발생하였고 마디수는 우람, 천상에서 가장 많았다. 2. 대부분 품종에서 6월 20일 파종 시에 가장 많은 협수 를 기록하였으나 대풍, 우람, 천상은 7월 5일 파종 시 에 오히려 협수가 많았다. 3. 만파 시 백립중은 적기 파종과 비교하였을 때 감소하 였으며 수량은 적기 파종과 만파 시 모두 대풍, 태광, 우람 순으로 가장 높았다. 4. 만파에 따른 수량감소율은 남풍이 9.6%로 가장 낮아 만파적응성이 가장 높은 품종인 것으로 보인다. 5. 파종기에서 개화기까지의 기간은 파종기가 늦춰지더 라도 비교적 일정하였으나 개화기에서 성숙기까지는 만파할수록 크게 단축되었다. 중만생종 중 선유, 조생 종 중 황금올은 등숙기간의 단축이 가장 적어 만파에 따른 영향을 가장 적게 받는 것으로 나타났다.

‘조양1호’는 1999년 내병 다수성인 ‘풍산나물콩’을 모본으로, ‘수원187호’를 부본으로 하여 교배하여 계통육종법으로 육성한 품종이다. 유한신육형이며 꽃은 자색이고 잎은 피침형이며 모용은 회색이다. 종자의 종피와 배꼽은 황색이며 성숙한 꼬투리는 잘 터지지 않는다. 개화와 성숙이 빠르며 평균 성숙기는 9월 27일로 ‘풍산나물콩’보다 10일 빠르다. 경장은 51 cm로 다소 작으며 도복에 강하고, 불마름병과 뿌리썩음병, 성숙기 탈립에 강하다. 바이러스 접종시 괴저가 발생하나 포장에서는 강하며 종자병해는 ‘풍산나물콩’과 비슷하다. 종실 100립중은 11.6 g이며 발아율이 높고 경실종자가 거의 발생하지 않아 ‘풍산나물콩’에 비해 콩나물 수율이 11% 높다. 종실 수량성은 전국 4개소에서 3년간 시험한 결과, 3.03MT/ha로 ‘풍산나물콩’보다 3% 증수하였다.

Soybean [Glycine max. (L.) Merr] is one of the most important legumes in the world. However, soybean varieties are sensitive to flooding stress and their seed yields are substantially reduced in response to the flooding stress. 192 soybean germplasm collection was screened to identify flooding tolerant germplasm at an early vegetative growth stage (V1). Soybean plants at V1 stage were waterlogged for 4 to 10 days. To evaluate flooding tolerance, survival rate were investigated as a time dependent manner. Jangbaegkong, Danbaegkong, Sowonkong, Socheong2 and Suwon269 showed flooding tolerance, while Shillog, T201, T181, NTS1116 and HP-963 showed flooding sensitivity. We also investigated effects of flooding stress on soybean morphology. The adventitious root development was greatly increased in flooding tolerant plants compared to it in flooding sensitive plants. In addition, root length and root number were analyzed. The significant reduction of root length and root number was observed in flooding sensitive plants. Thus, these results indicate that the morphological changes in roots are important for acclimation to flooding stress. Taken together, the relationship between the morphological changes in the roots and flooding tolerance may be useful in selecting a flooding tolerant soybean germplasm.

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and lethal necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Opmtimal reaction condition was at 58℃ for 60min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

Generally, the virus was detected by the ELISA using the serological method and RT-PCR based on the genetic information. Recently, NGS (next-generation nucleotide sequencing) has been used in genome analysis and diseases diagnostics. To identify distribution aspects of viruses, we collected diseased samples twice in soybean breeding field. After extraction of total RNA from the collected bulk samples, RNA was sequenced by the NGS method. The NGS data were analyzed using the bioinformatics software. With newly produced NGS data, the identification of distribution aspects of organisms in field was estimated in this study. Sequence based identification method should be more accurate diagnostic tools of the target diseases and be able to predict occurrence of potential and new pathogens. NGS method will also provide the basic data by identifying the distribution of using bacteria. In this study, we analyzed the extracted RNA from the collection of approximately 3000 samples. Consequently, we confirmed the following types: the 7,089 kinds of bacteria including Burkholderiaceae, the 13,397 kinds of Eukaryota, the 952 kinds of viruses from the first bulk samples, the 4,160 kinds of bacteria including Burkholderiaceae, the 10,475 kinds of Eukaryota, and the 576 kinds of viruses from the second bulk samples

In southern region of Korea, double cropping system including soybean as a second crop is widely adopted because prolonged high temperature in October enables soybean maturation even in late autumn. Global warming during decades derives transition of soybean planting dates as late as late-July. Delayed planting beyond the critical time moves reproductive stage to less favorable weather conditions like low temperature and short photoperiod, which can affect soybean yield and seed composition. Therefore selection of appropriate cultivar for changed climate and cultural practice is required. Effects of different eco-type and planting date on soybean yield was investigated for three sowing dates of 20 June, 5 July and 20 July with ten cultivars. Strong interaction between cultivar and sowing date was detected in number of branch, node and pod. No significant interaction was observed in 100-grain weight and yield, and this might be resulted from similar yield reduction pattern. Daepung, Taekwang and Uram recorded the highest yield when planted late, proving that these three cultivars are the most adaptable to delayed planting. Delayed planting results in higher sugar content, especially stachyose and raffinose which have detrimental effect on human health by causing flatulence and diarrhea. Protein content was decreased and oil content was increased in July 20 planting. Decrease in protein content was the largest in Hwangeumol and maintained high in Saedanbaek. This information will provide a guideline when select appropriate soybean cultivar in delayed planting to maintain yield and seed quality.