아열대 지역 적응 자포니카 중간모본 밀양375의 주요 농업 적 특성 및 품질관련 특성을 분석한 결과는 아래와 같다. 1. 아열대지역인 국제미작연구소에서 밀양375는 파종 후 85 일에 출수하였다. MS11보다 11일정도 늦었고, 아열대지역의 표준품종인 IR64호와 비슷하였다. 2. 주요 농업적 특성 및 수량성을 분석한 결과는 밀양375의 간장은 74.9 cm로 자포니카 품종인 MS11보다 약 5.2 cm 정도 큰 편이였고, 쌀수량은 346 kg/10 a로 MS11의 수량 262 kg/10 a보다 32% 증수되었고, IR64의 수량 337 kg /10 a와 비숫하였다. 3. 주요 품질관련 특성을 분석한 결과, 밀양375는 장폭비 1.78정도의 단원립이면서, 단백질 함량이 낮고, 아밀로스 함량 이 낮아 찰기가 양호하며, 밥의 윤기가 높은 계통이였다. 4. 주요 농업적 특성의 상관분석 결과 쌀수량과 출수기 (0.899). 수당립수(0.851), 간장 (0.832) 및 수장(0.685) 순으로 정의 상관관계를 나타내었고, 수수와 천립중은 부의 상관관계를 나타내었다. 5. phylogenetic tree를 분석한 결과는 밀양375는 국내 육성 자포니카 중만생종과 같은 그룹으로 분류되었으며, 아열대 지역 적응 자포니카 품종인 MS11과 다른 그룹으로 분류되었다. 따라서, 아열대 지역 자포니카 품종 육성을 위한 새로운 중간 모본으로 활용성이 높은 것으로 판단된다.

식물체 내에서 HPPD(4-hydroxy phenylpyruvate dioxyge-nase)를 저해하여 카로티노이드 생합성을 억제하는 제초제benzobicyclon 및mesotrione+pretilachlor의 처리 약량에 따른통일형 벼의 약해 발생 정도와 약해발생에 따른 수량영향을조사한 결과는 다음과 같다. 1. Benzobicyclon과 mesotrione+pretilachlor(MP) 처리 후6~12일에 통일형 벼인 한아름벼, 한아름2호, 다산벼는 모두잎의 백화약해 증상이 나타났고, 기준량 처리시 약해는 각각34~64%, 63~75%이었으며, 배량 처리에서는 약해발생이 더심해 각각 76~83%, 83~89% 이었다. 또한 벼식물체는 기준량 처리시 고사되지 않았으나 배량 처리시 식물체 고사율은benzobicyclon은 6.5~11.5%로 MP 2.1~3.3% 보다 고사율이 높았다.2. 출수기는 benzobicyclon 및 mesotrione+pretilachlor처리에 따른 약해로 대조인 butachlor 처리보다 1~2일 지연되었다.3. Benzobicyclon과 mesotrione+pretilachlor 처리시 통일형 벼 생육은 butachlor 처리에 비해 한아름벼, 한아름2호 및 다산벼 모두 기준량 처리시 m2 당 수수가 14~67개가 적고 등숙비율이 2.7~8.6% 낮아져으며 현미천립중이 0.0~1.2g 가벼웠다.4. Benzobicyclon과 mesotrione+pretilachlor 처리시 쌀 수량은 대조인 butachlor처리의 644~725kg/10a에 비해 한아름벼는 5~9%, 한아름2호와 다산벼는 5~8% 감소되었고, 2배량 처리시 수량감소는 더욱 증가하여 한아름벼는 16~28%,한아름2호는 20~24%, 다산벼는 14~23% 감소하였다.

Rice production is largely affected by various environmental conditions such as cold, heat and flooding. Here, to identify cold tolerant QTLs at seedling stage in rice, we generated RIL population derived from a cross between Hanareum 2 and Unkwang which are a highly cold sensitive and cold tolerant, respectively. We observed cold phenotype of this population in the growth chamber conditions and natural field conditions. For observation of cold tolerant phenotype of RIL population in the growth chamber, we treated cold stress (5~13℃) for 14 days and recovery for 4 days. When we examined the phenotype of RIL in the field conditions, temperature range in the field conditions was about 6 to 25℃ in 2015~2016. We named QTLs as Seedling Cold Tolerant (SCT) in growth chamber and Cold induced Yellowing Tolerant (CYT) in the field, respectively. Three QTLs for SCT and 5 QTLs for CYT were detected on chromosome 1, 6, 7, 8, 10, 11 and 12. Among these QTLs, qSCT12 on chromosome 12 showed 26.3 LOD score with 25.5% of phenotypic variation. When qSCT11.1 and qSCT12 were combined, cold tolerant was most strongest in our experimental conditions. qCYT10 on chromosome 10 was identified in field experiment on both 2015 and 2016. These results may provide useful information for a marker-assisted breeding program to improve cold tolerance in rice.

To fit the market demands for functional rice such as black and red color rice, ‘Sugary’a high free sugar content line was backcrossed to the black rice ‘Milyang152. The pedigree method was adapted in selection and generation advance. A high polyphenol content line, YR19646-3-2-1-1-2-2 showing red pericarp was selected and designed as ‘Milyang234’ followed by variety name ‘Geonganghongmi’ in 2010. Heading date of ‘Geonganghongmi’ was Aug. 19, medium-late maturing cultivar. ‘Geonganghongmi’ showed resistance to leaf blast and rice stripe virus while susceptible to major diseases and insects. The total polyphenol content of ‘Geonganghongmi’ was high as much as 24.2mg/g compare to that of 4.9mg/g of ‘Nampyeongbyeo’ and 17.2mg/g of a red pericarp rice ‘Jeogjinju’ at 70% ethanol extraction conditions. In local adaptability tests, yield of ‘Geonganghongmi’ was 4.5MT/ha about 86% of ‘Nampyeongbyeo’ and 102% of ‘Jeogjinju’, respectively. ‘Geonganghongmi’ is suitable for southern and middle plain area of Korea.

Blast resistance of 29 rice cultivars confirmed as a durable resistance in the evaluation of sequential planting from 2004 to 2006 was evaluated to nursery screening in 14 test sites during 11 years in Korea. The average disease severity (ADS) of 29 rice varieties against rice blast showed 3.5 degree; however, the difference of disease severity among the varieties was from 1.9 to 4.8. The 29 varieties were grouped into resistance less than 3.0 ADS degree including 12 varieties and moderate resistance over 3.1 ADS degree including 17 varieties. Among the 12 rice cultivars presented low ADS, 4 rice cultivars, Ungwang, Pungmi 1, Sinunbong 1, and Dasan 1 were constantly appeared high resistant reaction during 11 years in all test sites and the others were showed various diseases severity across the test years and the test sites. Twenty-one rice cultivars including Gopum were more variable among the test sites while the others were higher variable among the test years. These results indicated that durable resistance test against rice blast using sequential planting is a very efficient screening method to predict durability and nursery test for long periods and also useful method to predict indirectly durable resistance of rice cultivars.

Jungmo1019 early maturing rice variety was developed by the rice breeding team of the Department of Southern Crop, NICS, RDA in 2011. This cultivar was derived from the cross Hwayeongbyeo//Hitomebore/Nampyeongbyeo made in 2005 and the production of doubled haploid rice plant from anther culture in F1 generation. Testing in the replicated yield trial in 2008, a promising line YR25312Acp62 was selected and it was designated as the Milyang246. The early traus plauting local adaptability test of Milyang246 was carried out at three location in 2011 and it was named as Jungmo1019 showing a high head rice ratio and early–maturity eco-type. Number of spikelet/panicle and 1,000 grain weight of brown rice was slightly lower than those of Jopyeongbyeo. This variety is resistant to bacterial blight, rice stripe virus disease but susceptible to major insect and pests. The milled rice yield of Jungmo1019 is 4.52MT per ha at the early transplanting of the local adaptability test. This line should serve as a useful source for improving head rice ratio at early transplanting cultivation in southern plain area.

The amylose contents of rice determine eating quality which is one of the major traits in rice breeding program. To identify the low-amylose gene of the japonica rice cultivar Baegjinju, genetic analysis was conducted using 200 F2 population derived from a cross between the japonica cultivars, Saeilmi and Baegjinju. Individual F2 plants were classified as wild type (translucent grain) and mutant type (dull grain) based on the grain appearance of brown rice. Two hundred F2 plants were segregated into 155 wild type plants and 45 mutant type plants, which fit the 3:1 ratio (x2 = 0.667, df = 1, p = 0.414) and this result indicated the low-amylose gene of Baegjinju is a single recessive gene which controls the amylose contents. Linkage analysis was conducted to localize the low-amylose gene of Baegjinju and fine mapped within an 800-kb interval between 17.5 to 18.8Mb on short arm of chromosome 10. Co-segregated SSR marker, RM25648 was developed and it could be useful for marker-assisted selection and determination of the genetic resource related with amylose contents in rice breeding.

Bakanae (foolish seedling) disease caused by Gibberella fujikuroi creates serious problems in the foremost rice growing countries. This study was conducted to identify new resistance genetic sources to Bakanae disease. Bioassay showed that 11 varieties including Gwangmyeongbyeo, Hawn, Wonseadaesoo, Erguailai etc. were resistant to bakanae disease among 254 rice germplasm. Mismatch ratio between phenotype on bakanae disease bioassay and allele type of RM9, a SSR marker closely linked the bakanae disease resistant QTL, qBK1, were 38.3%. These results suggest that RM9 might be used for selecting qBK1, but it cannot be used for wide range of rice germplasm. Resistant germplasm in this study might be have resistant genes different from qBK1. The eleven varieties resistant to selected in this study will be used to identify new resistant alleles or genes to improve bakanae disease resistance in rice.

Drought caused by global climate change is one of serious problems for rice cultivation. However, it was little reported the impact of drought on rice cultivation in Korea. In here, to assess impact of drought on rice varieties in Korean climate condition, growth characteristics and yield components of rice were compared on irrigated and partially irrigated rice paddy field. First, we have chosen 11 rice varieties including ‘Saeilmi’ and ‘Shindongjin’ which are widely cultivated in Korea. For partially irrigated rice paddy treatment, we have withheld irrigation from 25 days after transplanting and water supply was totally dependent on rainfall for rice cultivation. When we examined early plant height and tiller number of these varieties on partially irrigated rice paddy were reduced 1.6% to 18.4% and 10.4% to 33.1%, respectively, and these reduction rate were highly correlated with yield loss in our experimental conditions. Among rice yield components, panicle number was decreased 10.5% to 30.1% according to rice varieties and reduced panicle number was highly correlated with yield loss. Grain number per panicle, grain filling rate and 1,000 seeds weight did not have correlation with yield loss of rice varieties. These result means that growth stage, especially the tillering stage, is seriously affected by drought on rice cultivation in Korea. And we suggest that ‘Saeilmi’, ‘Ilmi’ and ‘Ilpum’ are good for rice cultivation on drought prone rice field in Korea.

저글루테린 및 저아밀로스 품종인 ‘건양2호’는 ‘중국173 호’와 ‘주남벼’를 각각 모본과 부본으로 인공교배 후 집단육 종법을 통하여 육성한 기능성 품종이다. ‘건양2호’의 출수기 는 영호남 및 중부평야지 보통기 보비재배에서 8월 10일로 ‘남평벼’보다 5일 빠른 중생종이다. 간장은 65 cm로 내도복 성이며, 불시출수와 수발아는 안되는 편이고 내냉성은 ‘남평 벼’와 비슷한 편이다. 잎도열병 밭못자리검정에서 중정도 저항성을 보였으며, 줄무늬잎마름병과 흰잎마름병(K1, K2, K3) 에는 저항성이다. ‘건양2호’의 아밀로스함량은 11.5%로 메벼 와 찹쌀의 중간형태로 불투명한 저아밀로스 품종이며, 단백질 함량은 6.0%로 ‘남평벼’보다 약간 낮으며 소화성 전분인 글 루테린 비율이 63.1%로 ‘남평벼’의 73.8%보다 낮다. ‘건양2 호’의 쌀 수량성은 4.61 MT/ha로 ‘남평벼’보다 9% 감수되었 고, 적응지역은 영호남 및 중부 평야 1모작지이다(품종 등록 번호: 제5105호).

‘단미’는 국립식량과학원에서 1994년 당질미 유전자원인 ‘Sugary’를 모본으로 하고, 재배 안정성이 높은 ‘섬진벼’를 부본으로 인공교배하여 육성한 품종으로 현미의 유리당 함량 이 높으며 중부 및 영호남 내륙 평야 1모작지에 적응하는 중 만생 기능성 품종이다. ‘단미’의 간장은 86 cm로 ‘남평벼’보 다 12 cm 정도 큰 편이며, 이삭당 착립수가 적고 등숙비율 및 현미천립중은 각각 11.3% 및 16.1 g으로 매우 낮은 편이다. ‘단미’는 좌절중이 적고 3절 간장은 큰 편으로서 도복지수가 높으나 천립중이 가벼워 도복은 발생하지 않았으며 수발아는 약한 편이다. 내병충검정에서 도열병 및 흰잎마름병과 줄무늬 잎마름병, 오갈병 및 검은줄오갈병 등 바이러스 및 벼멸구와 애멸구 등 병해충에 대한 저항성은 없다. ‘단미’의 현미 유리당 함량은 Fructose, Glucose, Sucrose, Maltose, 및 Raffinose 가 각각 1.16, 7.87, 9.69, 0.52, 및 2.13%로 ‘남평벼’의 총 유 리당 함량 3.33%에 비해 6.5배 정도 높은 21.73%의 총 유리 당 함량을 보였다. 수량성은 2006년부터 2008년 3개년간 실 시한 지역적응시험 보통기 보비재배 6개소에서 현미 수량은 2.7 MT/ha로서 일반 메벼인 ‘남평벼’ 대비 약 52% 수준을 보였다(품종 등록번호: 01-0001-2010-6).

In here, we screened drought tolerant varieties with modified leaf water loss rate assay and visual drought tolerant phenotype in the greenhouse conditions with more than 800 varieties. Among these varieties, Samgang, Gumei4 and Apo showed the lowest of leaf water loss rate and strong drought tolerant phenotype. To identify drought QTLs with Samgang variety, we developed the doubled-haploid (DH) population consist of 101 lines derived from a cross the drought tolerant cultivar Samgang and the drought sensitive cultivar Nagdong. To score the drought phenotype degrees of this population, we withheld water for 6 weeks and treated the watering for 7 days. After watering, visual phenotype was observed 1 to 9 degree according to the standard evaluation system for rice, IRRI. Drought sensitive parent Nagdong was almost died and was scored as 9 degree, while tolerant parent Samgang showed slightly leaf tip drying phenotype and was scored as 3 degree in our experimental conditions. Three main QTLs were detected on chromosome 2, 6, and 11 with this visual phenotype. We also measured relative water contend of these population under drought stress conditions, and got one main QTL on chromosome 11. The QTL loci on chromosome 11 with flanking markers RM26755-RM287 has a function for visual phenotype and relative water content under drought conditions.

Rice is a staple food crop for more than half of the world population. Severe losses of rice production was caused by various environmental conditions such as cold, heat and flooding annually. Rice is a highly sensitive to low temperature below 15-20 ℃ because of originating from tropical or subtropical climates. Especially, seedling of rice is easily damaged to low temperature and result in seedling yellowing, growth retardation, reduced tillering and yield losses at last. We used a recombinant inbreeding lines (RIL) population of 384 individuals derived from a cross between Hanareum 2, a highly cold sensitive variety and Unkwang, a cold tolerant variety for molecular mapping of QTLs related to cold tolerance. Seedling discoloration of each lines and parents caused by cold response were investigated in field condition after transplanting. And leaf samples of RIL population were collected for evaluation of chlorophyll content using 80% acetone extraction. The seedling of each lines and parents was subjected to low temperate by 5~13 ℃ during 14 days. The cold recovery score (CRS) of RILs was recorded after 4 days recovery period according to standard evaluation system (SES, IRRI). Total of eight QTLs were detected on chromosome 1, 7, 8, 10, 11 and 12 using cold tolerance traits, chlorophyll content, seedling discoloration and cold recovery score in 384 RILs. The qCRS12, which detected on chromosome 12 between two flanking markers id12002113, id12002563 (1.1 Mbp) showed 25 LOD score with 26% of phenotypic variation of cold recovery score in RILs population. The positive allele contributing to cold tolerance came from the cold tolerant parent Unkwang. The result may provide useful information for a marker-assisted breeding program to improve cold tolerant in rice.

Recently, concerns have been voiced about the high eating quality rice in the market. To cope the high quality demands in rice market, a rice cultivar ‘Jinsumi’ which is showing high grain quality and multi-resistant to diseases was developed by the National Institute of Crop Science, RDA, Miryang, Korea. ‘Jinsumi’ was derived from a three-way cross of ‘Milyang165’and F1 plant of YR16195-B-B-B-21-1/Milyang169. The pedigree of ‘Jinsumi’ was YR21999-B-B-B-17-3-1 and designated as ‘Milyang 218’ in 2005. Heading date of ‘Jinsumi’ was August 15 as mid-late maturing ecotype. ‘Jinsumi’ showed multi resistant to rice stripe virus (RSV), bacterial blight races of K1, K2, K3 and moderately resistant to leaf blast disease. The grain appearance of ‘Jinsumi’ was translucent and showed 96.6% of head rice ratio. The milled rice yield of ‘Jinsumi’ was 5.74 MT/ha at ordinary transplanting of local adaptability test. ‘Jinsumi’ would be adaptable to the plain area of Yeongnam province in Korea.

눈큰흑찰은 최근 국민의 건강에 대한 관심에 대한 요구에 부응하기 위해 2012년 국립식량과학원 기능성작물부에서 육 성한 품종이다. 눈큰흑찰은 씨눈에 함유된 기능성 물질 함량 이 높은 벼 개발을 목적으로 get를 모본으로 하고 흑찰벼인 조생흑찰 부본으로 교배하여 계통육종법으로 육성되었다. 선 발된 YR25277-B-B-314-2 계통을 밀양263호로 계통명을 부 여하고 2010～2012년 3년간 지역적응시험 실시하여 2012년 12월 농촌진흥청 농작물 직무육성 신품종선정위원회에서 품 종으로 선정되었으며 눈큰흑찰로 명명하였다. 눈큰흑찰의 출 수기는 전국 평균 7월 30일로 조생흑찰과 비슷한 조생종이다. 벼 키는 65 cm로 조생흑찰과 비슷하며 포기당 이삭수와 이삭 당 벼알수도 각각 13개와 73개로 조생흑찰과 비슷하다. 천랍 중이 17.8 g으로 조생흑찰보다 1.7 g 정도 가벼운 편이다. 도 복 및 도열병에는 강하나 흰잎마름병, 바이러스병 및 해충에 약한 편이다. 쌀수량은 3.54 Mt/ha로 조생흑찰의 4.12 MT/ha 보다 낮은 편이다. 현미모양은 장폭비 1.93으로 조생흑찰과 비슷한 중장립형이며 종피에 안토시아닌이 함유된 흑색찰벼 이다. 눈큰흑찰은 거대배 품종으로 씨눈무게가 1.46 mg으로 조생흑찰 보다 3배 정도 무거우며, 이에 따라 씨눈에 많이 포 함된 GABA 함량이 높다. 눈큰흑찰의 적응지역은 중부 및 남 부 평야지이다.

새로 개발된 유색미 품종인 중모1020의 왕겨를 이용한 색소의 조성과 함량을 분석하고 생리활성을 검정한 결과, 중모1020의 왕겨로부터 최초로 페튜니딘 색소를 다량함유 하고 있음을 Mass와 UPLC등의 기기분석을 통해 동정하였 다. 중모1020의 색소 조성 중 C-3-G 함량은 29.6%인데 비해 Pt-3-G의 함량이 68.3%를 차지하여 C-3-G 함량이 82.3% 인 기존의 벼 색소의 조성과는 완전히 다름을 보였다. 추출 용매 조건에 따른 분석 결과, 조추출물 수율은 100% 메탄 올에 1.0% HCl을 이용하였을 경우 수율이 11.4%로서 가장 높음을 보였고, 다음이 80% 메탄올에 1.0% HCl용매로 추 출한 경우였다. 안토시아닌 색소 추출량 역시 1.0% HCl을 함유한 100% 메탄올과 80% 메탄올에서 각각 275.0±4.1, 258.0±1.8 mg/100 g으로 가장 많았다. 항산화 활성 비교에 서는 전체 안토시아닌 색소함량이 많고 폴리페놀 추출율이 높은 1.0% HCl을 함유한 80% 메탄올 용매 추출물에서 가 장 항산화 활성이 높음을 보였다.

The global rice reduction due to drought averages 18 Mt, especially, 23 Mha of rice fields in Asia are drought-prone. However, rice breeding programs focusing on drought resistance have made little progress to date. Because proper screening approaches with large scale were not developed to evaluate the drought tolerant degree. In here, we have developed of leaf water loss rate with plastic ware in dark conditions for large screening. Through this bioassay system, we examined drought phenotype degrees of 650 rice varieties. To validate whether this optimized bioassay system is corelated with drought phenotype, we chose 14 varieties having the lowest or highest of the water loss rate. We observed the visual drought phenotype and agricultural traits in green house and field conditions. Apo and Samgang having the lowest of leaf water loss rate showed drought tolerance phenotype, whereas Yeolbaeg and Milyang254 having the highest of leaf water loss rate showed drought sensitive phenotype. Apo displayed proper root length trait and Samgang showed good root dry trait in the greenhouse conditions. These results suggest that a simple screening procedure with water lose rate of leaves is effective to perform large scale screening for drought phenotype in rice.