To stabilize the supply of kimchi by extending the storage period of spring kimchi cabbage, this study manufactured kimchi from spring kimchi cabbage under varying storage conditions and periods, and analyzed their quality and sensory characteristics following the maturing period. Trimming loss was lowest in the group of plasma+reverse direction+predrying+ HDPE film processing. The salting yield of spring kimchi cabbage stored for 12 weeks was lower than that of spring kimchi cabbage stored for 6 weeks, and the kimchi yield was low in the pre-treatment group of spring kimchi cabbage stored for 12 weeks. The firmness was slightly different according to the storage period from one month of maturation. From the perspective of pH and acidity, the maturation in the reverse direction+pre-drying+HDPE film processing group was slower than that in the normal group (<0.05). In the sensory evaluation, the preference was increased in the low temperature storage processing group as the maturation period was increased (<0.05).

This study aimed to evaluate the fermentation characteristics of mixed Makgeolli with barley and wheats under, fermentation at different temperatures (20, 25, and 30℃) and time (3, 6, and 9 days). The pH and sugar of mixed Makgeolli were 3.68~4.26 and 12.30~17.31 ∘Brix, respectively. The total acidity showed proportionate increase with fermentation temperature and time; whereas, reducing sugar contents decreased with increasing fermentation temperature and time. The alcohol contents of mixed Makgeolli varied significantly by fermentation temperature and time (p<0.05). In terms of color values, the L value decreased with increasing fermentation temperatures, and a value increased significantly with increasing fermentation time. The number of microorganisms (yeast, total bacteria and lactic acid bacteria) in mixed Makgeolli decreased progressively with increasing fermentation temperature and time. Among the organic acids (citric, lactic, malic, and pyruvic acids) in mixed Makgeolli, citric and lactic acid was present at the highest concentrations. From these results, the optimum fermentation conditions of mixed Makgeolli with barley and wheat were 20~25℃ for 6 days.

This experiment was conducted to obtain information of feed value and fermentative quality of wheat cultivar, ‘Taejoong’ to confirm availability as a whole crop silage. As a result, the heading date of ‘Teajoong’ is April 27, and plant height is longer than whole crop barley, ‘Youngyang’ or whole crop wheat, ‘Cheongwoo’, spike length are also large, fresh and dry matter yield are also high. In case of feed value, ‘Taejoong’ had higher crude protein content than whole crop barley, ‘Youngyang’ or whole crop wheat, ‘Cheongwoo’, lower NDF and ADF contents, and significantly higher digestible nutrient contents(p<0.05). In case of fermentation qulity, pH of ‘Taejoong’ was 4.2, and lactic acid content was lower than ‘Cheongwoo’ silage and significantly higher than ‘Youngyang’ silage(p<0.05). Acetic acid content was significantly lower than ‘Youngyang’ and ‘Cheongwoo’ silage(p<0.01), butyric acid content was significantly lower than that of ‘Youngyang’ silage (p<0.05). The final Flieg's score showed that ‘Taejoong’ silage was the best. Also income of ‘Taejoong’ improved than ‘Youngyang’ or ‘Cheongwoo’. Therefore, ‘Taejoong’ is considered sufficiently available as whole crop for forage.

This study investigated the quality characteristics and biological activities of rice germ fermented by Bacillus spp. During the milling process, the contents of rice germ in the rice bran (Control) were adjusted to 30% (RG30) and 70% (RG70). The approximate composition, pH, total acidity, total soluble solid, total sugar, polyphenol and flavonoid contents were measured. DPPH radical scavenging activity, xanthine oxidase (XO) and angiotensin converting enzyme (ACE) activities were also determined. We observed that the moisture content decreased after fermentation, while the crude protein was significantly increased. Fermentation remarkably lowered the pH from 5.83~6.26 to 4.77~4.93, thereby elevating the total acidity. Fermentation also increased the total solid contents, from 0.40~0.87 °Bx to 1.63~2.20 °Bx. The total sugar decreased to 136.81~151.53 mg/mL from 377.56~450.64 mg/mL. Polyphenol contents were the highest in control (0.59 and 0.73 mg/mL before and after fermentation, respectively). Fermentation significantly affected the increase of the polyphenols in both rice germ 30% and 70% samples, from 0.26 and 0.28 mg GAE/g before fermentation, to 0.52 and 0.70 mg GAE/g after fermentation, respectively. There was a slight increase in the flavonoid contents after fermentation. The IC50 value of the electron donating ability, as evaluated by the DPPH method, was the lowest in control (3.77 and 3.36 mg/mL before and after fermentation, respectively). Fermentation increased the XO inhibition activity up to 63.69% in control, 49.81% in rice germ 30%, and 59.32% in rice germ 70%. The ACE inhibition activities were also increased in the fermented control, rice germ 30% and 70%, to 40.51%, 22.69% and 33.91%, respectively.

Effect of salt concentration (10, 20 and 30%, respectively) and fermentation temperature (10 and 20oC, respectively) on changes in quality index (VBN, Histamine, Amino nitrogen, Total viable cell counts, Coliform bacteria and E. coli counts) of culinary salted and fermented anchovy during fermentation were investigated to suggestion of fundamental documents for industrial objectives. Our results show that the effect of salt concentration on changes in quality index was not high compared with fermentation temperature in salted and fermented anchovy with below 20% of salt concentration however effect of salt concentration and fermentation temperature on quality index was not significant with 30% salt concentration. And all most whole changes of quality index were rapidly increased or decreased for 30 days of fermentation.

This study aimed to evaluate the quality characteristics of Gastrodia elata Blume fermented by lactic acid bacteria after saccharifying by 3 methods including enzyme, malt, and rice-nuruk. The lactic acid bacteria (LAB), Pediococcus inopinatus BK-3, isolated from kimchi could reduce the unpleasant taste and odor of Gastrodia elata Blume. The total acidity value of Gastrodia elata fermented by LAB on the malt and rice-nuruk extract solution for 3 days was 2.23% and 2.33%, respectively. After saccharification by malt and rice-nuruk extract solution for 3 days, the viable cell number of fermented Gastrodia elata was 9.14 log cfu/mL and 9.27 log cfu/mL, respectively. The total acidity values were increased above 3.35% by malt and rice-nuruk extract solution for 8 days. Thus, the viable cell number was the highest by malt and rice-nuruk extract solution fermentation for 3 days. The amino acid content of Gastrodia elata fermented by LAB after saccharification by malt extract solution was higher than that of other saccharifying methods. The free sugar content and p-hydroxybenzyl derivatives induced by the enzyme method were higher than those of other saccharifying methods. The overall acceptability was the highest at 4.2 point in Gastrodia elata fermented by malt extract solution.

Makgeolli is rich in nutrients such as beneficial microbial species, essential amino acids, oligosaccharides, and organic acids. The lactic acid bacteria contained in Makgeolli provides a healthy function as probiotics for customers. The purpose of this study was to enhance the nutritional properties, lactic acid bacteria propagation and alcohol contents of makgeolli by controlling redox potential balance. An automatic control system was created using ORP sensor, Labview control kit, and air supplier, in which air concentration was controlled by on & off mode. Makgeolli was fermented at three different redox potentials -50 mV, -100 mV, -150 mV. Regulating of aeration according to the redox potentials could give prominence to nutritional benefits of Makgeolli. The profiles of redox potential appeared bathtub curves, and related to lactic acid bacteria growth curve and byproducts. We could find the optimum redox potential balance that affects the factors such as LAB’s, alcohol contents and byproducts. In conclusion it was essential to control redox potential balance in order to produce nutrients rich Makgeolli.

The present study was carried out to explore the potential effects of novel lactic acid bacteria Pediococcus penticeous KCC-23 (KCC-23) and Lactobacillus plantarum KCC-24 (KCC-24) on rye haylage fermentation at National Institute of Animal Science, Cheonan province in Korea. The experiment contains three different groups such as control without lactic acid bacteria, rye haylage with KCC-23 and rye haylage KCC-24. After experimental periods, the content of crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF), the activity of total digestible nutrient (TDN) and In-vitro digestibility (IVD) was similar in control and LAB treated haylage (p<0.05). The pH was reduced in KCC-23 and KCC-24 treated rye haylage as compared to control (p<0.05). The lactic acid concentration in haylages was increased by L. plantarum KCC-23, and KCC-24 supplement. Whereas, the less amount of acetic acid and butyric acid was noted in KCC-23 and KCC-24 treated haylage as compared with control (p<0.05). The KCC-23 and KCC-24 were dominantly grown in experimental haylage as compared with control. It indicates, the addition of KCC-23, and KCC-24 enhances fermentation quality of haylages as compared control. The present study suggests that KCC-23 and KCC-24 are potent strains that were improving the fermentation process in rye haylage

본 연구에서는 호밀을 이용하여 사일리지 제조시 Lactobacillus plantarum KCC-10 및 KCC-19를 접종하여 사일리지의 사 료가치, 품질 및 미생물상의 변화를 조사하였다. 호밀 사일 리지에서 젖산균 접종구와 무접종구에서 사료가치는 크게 변화되지 않았다. 그리고 유산 함량은 젖산균 접종구가 현 저하게 높은 경향을 보였으나 초산과 낙산은 젖산균 접종 구가 약간 낮아지는 경향을 보였다. 젖산균 분포는 젖산균 접종구에서 현저하게 높았으며 효모와 곰팡이는 104CFU/g 에서 관찰되지 않았다. 호밀 사일리지 제조시 KCC-10 및 KCC-19 접종구의 조단백질 및 TDN 함량은 무접종구 보 다 약간 증가하였으나 섬유소 및 in vitro 건물소화율에서 는 비슷한 수준을 보였다. 이상의 결과를 요약해 보면 호 밀 사일리지 제조시 KCC-10 및 KCC-19 접종에 의해 사일 리지의 품질이 향상되었다.

An investigation was carried out to evaluate the potential role of Lactobacillus Plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of Italian ryegrass (IRG) silages. The physiochemical properties of IRG silage such as crude protein content, neutral detergent fiber, acid detergent fiber, total digestible nutrient and in vitro dry matter digestibility were not affected by KCC-10 and KCC-19. The pH of IRG silage in KCC-10 and KCC-19 treatments decreased compared to the control (p<0.05), while the lactic acid content in KCC-10 and KCC-19 treatments increased compared to the control (p<0.05). In addition, the number of lactic acid bacteria (LAB) in the KCC-10 treatment increased compared to the control (p<0.05). The number of lactic acid bacteria in KCC-19 increased, but there was no significant difference in all treatments. Therefore, we recommend L. plantarum KCC-10 and KCC-19 as potential additive candidates in IRG silage with lots of advantages.

본 연구는 충남 천안 국립축산과학원 식용벼 재배지에서 2012년부터 2013년까지 정상적으로 벼 재배가 이루어지고 수확이 완료된 볏짚을 이용하여 수행하였다. 젖산균 접종 유무에 따라 볏짚사일리지의 사료가치 변화는 나타나지 않았으나 클로렐라 처리구는 대조구에 비해 현저하게 조단백질 함량이 증가하였다(p<0.05). 그리고 클로렐라 농도의 증가에 따라 조단백질 함량이 증가하는 경향을 보였다(p<0.05). 모든 처리구에서 섬유소와 TDN 함량 및 in vitro 소화율은 차이를 나타내지 않았다. 그리고 젖산균 접종 유무에 따라 볏짚사일리지의 pH 및 유기산 함량 변화는 나타나지 않았으며 클로렐라 처리구도 무처리구에 비해 pH 변화는 나타나지 않았다. 그러나 클로렐라 처리구에서 젖산함량은 무처리구에 비해 유의적으로 증가하였으며 클로렐라 농도의 증가에 따라 젖산함량이 증가하는 경향을 보였다(p<0.05). 젖산균 단독접종구의 젖산균 수는 무처리구에 비해 현저하게 증가하였으며(p<0.05), 클로렐라 농도의 증가에 따라 증가하는 경향을 보였다(p<0.05). 그러나 효모와 곰팡이 수는 비슷한 수준을 나타냈다. 그러므로 볏짚사일리지의 사료가치와 품질은 클로렐라 첨가에 의해서 향상될 수 있다.

본 연구는 국내에서 헤일리지의 생산 및 이용 확대를 위해 수분함량에 따른 헤일리지의 사료가치 및 발효품질의 변화를 구명하고 양질의 헤일리지 생산 확대를 위한 기초 자료를 확보하기위하여 2011년 9월부터 2012년 12월까지 충남 천안시 소재 국립축산과학원 초지사료과 시험포장에서 수행되었다. 수분함량이 다양한 헤일리지의 조제를 위해 이탈리안 라이그라스의 ‘코윈어리’ 품종을 이용하여 수분함량 60~30%까지 5% 차이로 총 7처리로 헤일리지를 조제하였다. 수분함량이 다른 이탈리안 라이그라스 헤일리지의 사료가치는 수분함량에 따라 유의적인 차이를 나타내지 않았다. 수분함량에 따른 발효품질의 변화는 수분함량의 차이에 따라 유의적인 차이를 보였으며 상대적으로 수분함량이 높은 조건(>40%)에서 발효품질이 우수하였으며 수분함량이 낮은 조건에서는 발효품질도 낮은 것으로 나타났다. 수분함량별 헤일리지의 유기산 구성비율은 수분함량이 상대적으로 높은 조건(>40%)에서 젖산비율이 높게 나타났으며 수분함량이 낮은 조건(<40%)에서는 젖산비율이 감소하고 초산과 낙산비율이 높아지는 것으로 나타났다. 이상의 결과를 종합해 보면 안정적인 헤일리지 생산을 위해 발효품질을 고려해보면 수분함량이 40%가 헤일리지 조제의 한계점으로 사료되며 추후 헤일리지의 장기 저장성 검토 및 저 수분 조건에서 발효품질 개선을 위한 추가적인 연구가 진행되어야 할 것으로 생각된다.

이 연구는 비닐겹수 및 저장기간 따른 청보리 사일리지의 사료가치 및 사일리지 품질특성을 구명하고자 수행하였다. 조단백질 함량은 저장기간 모두 사일리지 제조 전에 비해 높아지는 경향을 보였고, 저장기간이 길어질수록 약간 높은 값을 보였지만 통계적인 유의성은 없었다. 비닐겹수에 따라서는 6겹 처리한 처리구가 4겹보다 높은 경향이었다. NDF와 ADF 함량은 저장기간 모두 사일리지 제조 전보다 높은 경향을 보였고(p<0.05), 저장기간에 따라서는 비슷한 값을 나타냈다. 비닐 겹수에 따라서는 6겹 처리한 처리구가 4겹보다 더 높은 값을 나타냈다. TDN 함량은 저장기간 모두 사일리지 제조 전보다 낮은 경향을 보였고, 저장기간에 따라서는 비슷한 값을 보였다. 비닐겹수에 따라서는 6겹에서 다소 낮은 값을 보였다. pH는 저장기간이 길어짐에 따라 낮아지는 경향을 보였고, 비닐겹수에 따라서는 6겹에서 4겹보다 낮은 값을 보였다. 유기산 함량에서는 저장기간이 길어짐에 따라 젖산함량은 약간 증가하는 경향을 보였고, 초산함량은 낮아지는 경향을 보였으며 낙산함량은 유의적으로 증가하는 경향을 보였다(p<0.05). 비닐겹수에 따라서는 6겹에서 4겹보다 높은 젖산함량과 낮은 낙산함량을 보였다(p<0.05). 따라서 청보리 사일리지는 장기저장을 할 경우에는 6겹으로 하는 것이 유리하고, 4겹으로 처리했을 경우 6개월 안에 사용하는 것이 유리하다고 판단된다.

이 연구는 비닐겹수 및 저장기간 따른 청보리 사일리지 의 사료가치 및 사일리지 품질특성을 구명하고자 수행하였 다. 조단백질 함량은 저장기간 모두 사일리지 제조 전에 비해 높아지는 경향을 보였고, 저장기간이 길어질수록 약 간 높은 값을 보였지만 통계적인 유의성은 없었다. 비닐겹 수에 따라서는 6겹 처리한 처리구가 4겹보다 높은 경향이 었다. NDF와 ADF 함량은 저장기간 모두 사일리지 제조 전보다 높은 경향을 보였고(p<0.05), 저장기간에 따라서는 비슷한 값을 나타냈다. 비닐 겹수에 따라서는 6겹 처리한 처리구가 4겹보다 더 높은 값을 나타냈다. TDN 함량은 저 장기간 모두 사일리지 제조 전보다 낮은 경향을 보였고, 저장기간에 따라서는 비슷한 값을 보였다. 비닐겹수에 따 라서는 6겹에서 다소 낮은 값을 보였다. pH는 저장기간이 길어짐에 따라 낮아지는 경향을 보였고, 비닐겹수에 따라 서는 6겹에서 4겹보다 낮은 값을 보였다. 유기산 함량에서 는 저장기간이 길어짐에 따라 젖산함량은 약간 증가하는 경향을 보였고, 초산함량은 낮아지는 경향을 보였으며 낙 산함량은 유의적으로 증가하는 경향을 보였다(p<0.05). 비 닐겹수에 따라서는 6겹에서 4겹보다 높은 젖산함량과 낮은 낙산함량을 보였다(p<0.05). 따라서 청보리 사일리지는 장 기저장을 할 경우에는 6겹으로 하는 것이 유리하고, 4겹으 로 처리했을 경우 6개월 안에 사용하는 것이 유리하다고 판단된다.

This study was conducted to investigate the effects of bacterial inoculation (Lactobacillus plantarum) and cutting height on the chemical composition, fermentation characteristics and in vitro dry matter digestibility (IVDMD) in whole crop barley silage. Barley forage (Youngyang hybrid) was harvested at about 27% of dry matter (DM) level at two different cutting height (5 vs. 15 cm). And it was chopped to 5 cm length and treated with or without L. plantarum. Four replicates of each treatment were ensiled into 10 L mini silo (3 kg) for 100 days. After 100 days, bacterial inoculation decreased (p=0.001) DM content, while increased cutting height increased (p=0.002) DM in uninoculated silage. Crude protein (CP) concentration was decreased by increasing height in uninoculated silage (8.84 vs. 8.16) but increased in inoculated silage (8.19 vs. 8.99). Both neutral detergent fiber (NDF) (p<0.011) and acid detergent fiber (ADF) (p<0.004) were decreased by increasing cutting height of forage at harvest. The IVDMD and ammonia-N was increased (p=0.001) by increasing cutting height and inoculation, respectively. Lactic acid bacteria (LAB) was increased (p=0.002) in inoculated silage, but yeast count was decreased (p=0.026) in uninoculated silages. It is concluded that increased cutting height of forage at harvest could be useful to make a fibrous portion with increase of dry matter digestibility of silages.

본 연구는 들기름을 농도별로 배추김치에 첨가하여 6주의 발효기간 동안 pH, 산도, 젖산균수, 산가, 과산화물가, 색도 등의 품질특성과 관능특성을 조사하였다. 또한, 들깨로부터 볶지 않고 냉압착한 들기름과 볶은 후 가열 압착한 들기름을 같은 농도별로 배추김치에 첨가하여 분석하였다. 모든 실험군에서 발효가 진행됨에 따라 pH는 감소하였으며 6주 후에는 최적 pH인 4.48~5.38 범위였고, 산도도 점차 증가하여 1.20~2.09% 범위였다. 젖산균 수는 발효 0~1주 사이에 급격하게 증가하여 발효 6주에는 4.48~6.04 log CFU/g에 도달했으며, 냉압착 및 가열압착 들기름을 첨가하였을 때 젖산균이 더 증가하였다. 산가와 과산화물가는 발효기간에 따라 점차 증가하였으며, 들기름 첨가비율이 높아짐에 따라 증가하는 경향을 보였다. 가열압착 들기름 첨가군에 비해 모든 실험군에서 발효 기간 경과와 더불어 색도 중 L 값과 a 값은 점차 증가하였으며 b 값은 1주까지는 증가하다 점차 감소하는 경향이었다. 들기름을 첨가하여 제조한 김치의 관능평가를 한 결과 5% 냉압착 들기름을 첨가하였을 때 관능성이 가장 우수하였다. 이상의 결과들을 종합하면 배추김치 제조 시 냉압착한 들기름의 첨가는 발효 시 유용한 젖산균을 증가시키고 관능특성을 향상하는 것으로 판단된다.